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Cat. No.
 Name
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M0100
 Multiple Tag Cell Lysate
200 ?g
$72
Product Name Multiple Tag Cell Lysate
Species
 E. coli
Documents
Document-PRODUCTINFO: 2382_20060922114657.PDF (PDF)
Figures
Reference
Description
 Multiple Tag Cell Lysate is whole E. coli cell lysate comprised of about 30% specially designed fusion protein, which act as a positive control for Western blot or Dot blot. The fusion protein is a 52 kD protein containing 16 commonly used tags, which are, in order, Trx, T7, HSV, C-Myc, SVS-g, Glu-Glu, V5, E tag, DYKDDDDK, S-tag, HA, KT3, E2, AU1, AU5, and His tag.
Concentration
 200 µg of whole E. coli cell lysate (lyophilized)
Recommended loading volume
 Add 200 µl of ddH2O to make the final concentration of 1 µg/µl in 1X SDS Loading Buffer. 0.02 – 0.5 µl of the lysate is recommended for each lane of a minigel. For accurate loading, dilute with 1X SDS Loading Buffer.
Storage
 Store at -20°C or below. After reconstitution, aliquot and store at -20°C or below.
Application
 Positive control for Western blot and Dot blot.