| Product Name |
PCR DNA Ladder |
Full Name |
PCR DNA Ladder |
|
Description |
PCR DNA Ladder |
Concentration |
400 ng/5 μl in 10 mM Tris-HCl (pH7.5), 1 mM EDTA. For ready-to-useTM format, 1X loading buffer has been added to the marker, and the marker is ready for loading to the gel. |
Note |
⢠6X DNA loading buffer is available separately. ⢠Ready-to-useTM format is premixed with DNA loading buffer containing 5% glycerol, 0.02% bromophenol blue, and 0.02% xylene cyanol. |
Recommended loading volume |
5 μl (or 1 μl/mm width of gel well) |
Storage |
Store at -20°C |
Quality Control |
• Agarose gel analysis shows that the bands between 100 bp and 2 kb are accurate in size and readily distinguishable. The 500 bp band is at least three times more intense than any other band. • Incubation of 5 μl of the PCR DNA Ladder in restriction enzyme buffer overnight at 37°C shows absence of visible degradation. |