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Open Reading Frame (ORF) clones greatly facilitates biological research turn DNA sequences into expression-ready clones. GenScript ORF cloning services provide advanced Gene-on-Demand® technology platform and GenPoolTM ORF clones database with 2,428,863 ORF clones from 186 different species,and the number is growing.
- GenPoolTM ORF clones database:
Our ORF clones database includes over 2 million ORF clones from 186 different species, and provides various convenient search options.
Research Areas-- over 3,000 records
Protein Families-- over 1,000 records
Signaling Pathways-- over 2,000 records
Function Groups-- over 3,000 records
- Gene-on-Demand® gene synthesis technology platform:
Our proprietary platform is based on the following technologies: including GenPoolTM ORF clones database, OptimumGeneTM codon optimization software, vector collection and clone archive database.
- Expression-ready cloning:
ORFs can be cloned into our pDream vector,which is suitable for three different expression systems- bacteria, Sf9 cells, and mammalian cells. ORFs can also be cloned into other commercial or custom vectors.
- DYKDDDDK-tagged cloning:
ORFs cloned into pDream vector are fused with a DYKDDDDK tag, where expression can be detected by anti-DYKDDDDK antibody
(GenScript, A00187). This epitope tag is the most widely applied in structural and functional studies of proteins;
it can be easily removed by enterokinase (EK, GenScript, Z01003).
Pricing
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| Price per ORF cloning
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SC1200 |
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- Sequence Confirmation:
The sequences of your gene will be confirmed base-by-base against the complete ORF sequence. We will screen GenPoolTM ORF clones collection of full-length ORF clones database if no appropriate plasmid template is available.
- De Novo Synthesis or PCR Amplification:
GenScript now offers Gene-on-Demand® gene synthesis services for currently not included in our ORF clones database, by de novo gene synthesize or by PCR amplication.
- Cloning Vector:
The gene of your interest can be cloned into our pDream vector, or any commercial or non-commercial vector of your choice.
- QC by Sequencing:
Every clone will be fully sequenced before delivery to ensure 100% accuracy.
Delivery Specifications:
Our typical turnaround time is one week for ORFs cloned into pDream vector. If the ORF clone of your interest is not present in our database, or needs to be cloned into a vector other than pDream, the turnaround time is about 14 business days for genes up to 1 kb. Our typical delivery package includes the following:
- 10 μg lyophilized destination vector containing the gene of your interest
- Sequencing data of each clone (electronic version)
- Construct maps for the plasmid
- Quality assurance certificate
 Quotation and Ordering:
 For quotation requests and questions, you may contact us by phone, email, fax, or via our online quotation system.
Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) or credit card.
Please send your samples with full shipping information and PO number (if applicable) with the completed PCR Cloning Quotation Request Form to PCR Cloning/Subcloning Services, GenScript USA Inc., 860 Centennial Avenue., Piscataway, NJ 08854, U.S.
Our customer service representatives are available 24 hours a day, Monday through Friday;please contact us anytime for assistance.
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