Custom Polyclonal Antibody Services (Mouse) |
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Complete Peptide Polyclonal Antibody Package (Mouse) (Cat. No.: SC1046)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a, >85% purity, 7.5 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Five Balb/c mice, pre-immune bleed, four immunizations/mouse
- ELISA test
- Lyophilization
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- 50-100 µl of pre-immune serum
- ELISA results
- 2.5 ml of antisera (lyophilized) from all mice
- 5 mg of unconjugated peptide, MS and HPLC report
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Lyophilization and Shipping: 1-2 weeks
- Total 14-16 weeks
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Complete Protein A-Purified Peptide Polyclonal Antibody Package (Mouse) (Cat. No.: SC1049)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a, >85% purity, 7.5 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Five Balb/C mice, pre-immune, four immunizations/mouse
- Antibody purification by protein A column
- ELISA test
- Lyophilization (optional)
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- 50-100 µl of pre-immune serum
- ELISA results
- 2.5-12.5 mg purified antibody (liquid recommended) from all antisera
- 5 mg of unconjugated peptide, MS and HPLC report
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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Standard Protocol (Five animals)
| Steps | Time from
Project Initiation | Time from Immunization | Activity Description | | Peptide synthesis | ≈2 weeks |
| - |
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Peptide conjugation to carrier
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≈3 weeks
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-
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Control serum collection |
≈4 weeks
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Day 0
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Pre-bleed (≈0.1 ml serum) |
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Primary immunization
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≈4 weeks
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Day 1
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0.1 mg per mouse peptide-KLH conjugate, CFA, i.p.
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| 1st boost |
≈6 weeks
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Day 14
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0.1 mg per mouse peptide-KLH conjugate, IFA, i.p.
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| Test bleed and ELISA |
≈8 weeks
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Day 24
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0.01-0.02 ml serum per mouse. ELISA and response evaluation.
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| 2nd boost |
≈9 weeks
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Day 35
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0.1 mg per mouse peptide-KLH conjugate, IFA, i.p.
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| Test bleed and ELISA |
≈11 weeks
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Day 45
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0.01-0.02 ml serum per mouse. ELISA and response evaluation.
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| 3rd boost |
≈12 weeks
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Day 56
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0.1 mg per mouse peptide-KLH conjugate, IFA, i.p.
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| Production bleed |
≈14 weeks |
Day 66
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0.5 ml serum per mouse
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| Lyophilization |
≈15 weeks |
Day 73
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-
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| ELISA and shipping |
≈16 weeks |
Day 80
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Extend or terminate
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Note:
If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.
Quotations and Ordering:
 For quotation requests and questions, you may contact us by email, phone, fax, or via our secured online quotation system.
Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.
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Email: antibody@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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