Custom Polyclonal Antibody Services (Rabbit) |
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Complete Peptide Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1015)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a., >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- ELISA test
- Lyophilization
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- 2 ml of pre-immune serum
- ELISA results
- 100-120 ml of antisera (lyophilized) from two rabbits
- 5mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Lyophilization and Shipping: 1-2 weeks
- Total 14-16 weeks
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Complete Protein A-Purified Peptide Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1045)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a., >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- Protein A purification
- ELISA test
- Lyophilization (optional)
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- 1-2 ml of pre-immune serum*
- ELISA results
- 200-500 mg protein A-purified antibodies (liquid recommended)
- 5 mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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Complete Affinity-Purified Peptide Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1031)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a., >85% purity, 15 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- Peptide (5 mg) conjugated to affinity resin
- Affinity purified antibodies
- ELISA test
- Lyophilization (optional)
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- 1-2 ml of pre-immune serum*
- ELISA results
- 3-50 mg affinity-purified antibodies (liquid recommended)
- 5 mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ESLIA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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Phospho-Specific Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1044)
Includes: |
Delivery: |
Time Frame: |
- Synthesis of two peptides, one with a modified amino acid (<15 a.a, >90% purity, 15 mg) and the other without modified amino acid (<15 a.a, >90% purity, 11 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Prepare two affinity columns
- Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption.
- ELISA test
- Lyophilization (optional)
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- 1-2 ml of pre-immune serum*
- ELISA results
- 1-30 mg phospho-specific antibodies (liquid recommended) from two rabbits
- 5 mg of unconjugated phospho-peptide and 5 mg of unconjugated phospho-peptide, MS and HPLC reports
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- Peptide Synthesis: 2-4 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-18 weeks
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* For collection and purification of antibodies from separated
animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2
animals) are provided. For antibody purification from
pooled serum, 1 ml of pre-immune serum is provided.Standard Protocol (Two animals)
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Steps
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Time from
Project Initiation
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Time from Immunization
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Activity Description
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Peptide synthesis
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≈2 weeks
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Day -21
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-
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Peptide conjugation to carrier
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≈3 weeks
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Day -7
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-
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Control sera collection
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≈4 weeks
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Day 0
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Pre-bleed (≈2-3 ml serum)
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Primary immunization
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≈4 weeks
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Day 1
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0.5 mg per rabbit peptide-KLH conjugate, CFA, s.c.
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| 1st boost |
≈6 weeks
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Day 14
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0.5 mg per rabbit peptide-KLH conjugate, IFA, s.c.
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| Test bleed and ELISA |
≈8 weeks
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Day 24
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100 µl serum per rabbit. ELISA and immune response evaluation.
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| 2nd boost |
≈9 weeks
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Day 35
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0.5 mg per rabbit peptide-KLH conjugate, IFA, s.c.
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| Production bleed |
≈11 weeks
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Day 45
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10-20 ml serum per rabbit
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| 3rd boost |
≈12 weeks
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Day 56
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0.5 mg per rabbit peptide-KLH conjugate, IFA, s.c.
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| Production bleed |
≈14 weeks
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Day 70
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40 ml serum per rabbit
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| Lyophilization |
≈15weeks
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Day 77
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-
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| ELISA and shipping |
≈16 weeks
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Day 84
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Extend or terminate
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Note:
If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.
Quotations and Ordering:
 For quotation requests and questions, you may contact us by email, phone, fax, or via our secured online quotation system.
Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.
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Email: antibody@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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