You are here: Home - Fr » CRO Biologique pour la Découverte de Médicaments »    Services d’Anticorps » Services d'Anticorps Polyclonaux » Services d'Anticorps Polyclonaux (Lapin)
GenScript Share

Custom Polyclonal Antibody Services (Rabbit)

polyclonal antibody services quotation
Complete Peptide Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1015)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a., >85% purity, 10 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Two rabbits, pre-immune bleed, four immunizations/rabbit
  • Final bleed
  • ELISA test
  • Lyophilization
  • 2 ml of pre-immune serum
  • ELISA results
  • 100-120 ml of antisera (lyophilized) from two rabbits
  • 5mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization and ELISA: 10 weeks
  • Lyophilization and Shipping: 1-2 weeks
  • Total 14-16 weeks

Complete Protein A-Purified Peptide Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1045)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a., >85% purity, 10 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Two rabbits, pre-immune bleed, four immunizations/rabbit
  • Final bleed
  • Protein A purification
  • ELISA test
  • Lyophilization (optional)
  • 1-2 ml of pre-immune serum*
  • ELISA results
  • 200-500 mg protein A-purified antibodies (liquid recommended)
  • 5 mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 1 week
  • Lyophilization and Shipping: 1-2 weeks
  • Total 15-17 weeks

Complete Affinity-Purified Peptide Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1031)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a., >85% purity, 15 mg)
  • A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
  • Two rabbits, pre-immune bleed, four immunizations/rabbit
  • Final bleed
  • Peptide (5 mg) conjugated to affinity resin
  • Affinity purified antibodies
  • ELISA test
  • Lyophilization (optional)
  • 1-2 ml of pre-immune serum*
  • ELISA results
  • 3-50 mg affinity-purified antibodies (liquid recommended)
  • 5 mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ESLIA: 1 week
  • Lyophilization and Shipping: 1-2 weeks
  • Total 15-17 weeks

Phospho-Specific Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1044)

Includes:
Delivery:
Time Frame:
  • Synthesis of two peptides, one with a modified amino acid (<15 a.a, >90% purity, 15 mg) and the other without modified amino acid (<15 a.a, >90% purity, 11 mg)
  • A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
  • Two rabbits, pre-immune bleed, four immunizations/rabbit
  • Prepare two affinity columns
  • Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption.
  • ELISA test
  • Lyophilization (optional)
  • 1-2 ml of pre-immune serum*
  • ELISA results
  • 1-30 mg phospho-specific antibodies (liquid recommended) from two rabbits
  • 5 mg of unconjugated phospho-peptide and 5 mg of unconjugated phospho-peptide, MS and HPLC reports
  • Peptide Synthesis: 2-4 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 1 week
  • Lyophilization and Shipping: 1-2 weeks
  • Total 15-18 weeks
* For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided.

Standard Protocol (Two animals)

Steps
Time from
Project Initiation
Time from Immunization
Activity Description
Peptide synthesis
≈2 weeks
Day -21
-
Peptide conjugation to carrier
≈3 weeks
Day -7
-
Control sera collection
≈4 weeks
Day 0
Pre-bleed (≈2-3 ml serum)
Primary immunization
≈4 weeks
Day 1
0.5 mg per rabbit peptide-KLH conjugate, CFA, s.c.
1st boost
≈6 weeks
Day 14
0.5 mg per rabbit peptide-KLH conjugate, IFA, s.c.
Test bleed and ELISA
≈8 weeks
Day 24
100 µl serum per rabbit. ELISA and immune response evaluation.
2nd boost
≈9 weeks
Day 35
0.5 mg per rabbit peptide-KLH conjugate, IFA, s.c.
Production bleed
≈11 weeks
Day 45
10-20 ml serum per rabbit
3rd boost
≈12 weeks
Day 56
0.5 mg per rabbit peptide-KLH conjugate, IFA, s.c.
Production bleed
≈14 weeks
Day 70
40 ml serum per rabbit
Lyophilization
≈15weeks
Day 77
-
ELISA and shipping
≈16 weeks
Day 84
Extend or terminate

Note:

If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.

Quotations and Ordering:

molecuar biology services quotation

For quotation requests and questions, you may contact us by email, phone, fax, or via our secured online quotation system.

Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.

Email: antibody@genscript.com
Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Fax: 1-732-210-0262 1-732-885-5878