Custom Polyclonal Antibody Services (Rabbit) |
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1. SC1015: Complete Peptide Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a., >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- ELISA test
- Lyophilization
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- 2 ml of pre-immune serum
- ELISA results
- 100-120 ml of antisera (lyophilized) from two rabbits
- 5mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization and ELISA: 10 weeks
- Lyophilization and Shipping: 1-2 weeks
- Total 14-16 weeks
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2. SC1045: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a., >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune, four immunizations/rabbit
- Final bleed
- Protein A purification
- ELISA test
- Lyophilization
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- 1-2 ml of pre-immune serum*
- ELISA results
- 200-500 mg protein A-purified antibodies (lyophilized)
- 5 mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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3. SC1031: Complete Affinity-Purified Peptide Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Peptide synthesis (<15 a.a., >85% purity, 15 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- Peptide (5 mg) conjugated to affinity resin
- Affinity purified antibodies
- ELISA test
- Lyophilization
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- 1-2 ml of pre-immune serum*
- ELISA results
- 3-5 mg affinity-purified antibodies (lyophilized)
- 5 mg of unconjugated peptide, MS and HPLC reports
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- Peptide Synthesis: 2-3 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ESLIA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-17 weeks
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4. SC1044: Phospho-Specific Polyclonal Antibody Package (Rabbit)
Includes: |
Delivery: |
Time Frame: |
- Synthesis of two peptides, one is phospho-peptide (<15 a.a, >90% purity, 15 mg) and the other is non-phospho-peptide (<15 a.a, >90% purity, 10 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Prepare two affinity columns
- Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption.
- ELISA test
- Lyophilization
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- 1-2 ml of pre-immune serum*
- ELISA results
- 1-3 mg phospho-specific antibodies (lyophilized) from two rabbits
- 5 mg of phospho-peptide and 5 mg of non-phospho-peptide, MS and HPLC reports
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- Peptide Synthesis: 2-4 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-18 weeks
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5. SC1492: Modification-Specific Polyclonal Antibody Package (Rabbit)**
Includes: |
Delivery: |
Time Frame: |
- Synthesis of two peptides, one with a modified amino acid (<15 a.a, >90% purity, 15 mg) and the other without modified amino acid (<15 a.a, >90% purity, 10 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- Prepare two affinity columns
- Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption.
- ELISA test
- Lyophilization
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- 1-2 ml of pre-immune serum*
- ELISA results
- 1-3 mg modified-specific antibodies (lyophilized) from two rabbits
- 5 mg of modified peptide and 5 mg of unmodified-peptide, MS and HPLC reports
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- Peptide Synthesis: 2-4 weeks
- Conjugation: 1 week
- Immunization: 10 weeks
- Purification and ELISA: 1 week
- Lyophilization and Shipping: 1-2 weeks
- Total 15-18 weeks
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** These modifications include methylation, acetylation, nitration, etc.
* For collection and purification of antibodies from separated
animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2
animals) are provided. For antibody purification from
pooled serum, 1 ml of pre-immune serum is provided.
Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera or antibody products to prevent bacteria growth. Lyophilization extends the shelf life of your antibodies, eliminates leaking problems and loosens up the stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped.
Quotations and Ordering:
 For quotation requests and questions, you may contact us by email, phone, fax, or via our secured online quotation system.
Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.
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Email: antibody@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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