| Full Name |
Glucagon-Like Peptide-1 (GLP-1), Human
|
Abbreviated Name-1 |
Glucagon Like Peptide-1; rHuGLP-1 |
| Documents |
|---|
 Document-MSDS: 15104_20111229204518.PDF (PDF) |
| Figures |
|---|
| Reference |
|---|
Lee DH, et al. Proteome analysis of recombinant Escherichia coli producing human glucagon-like peptide-1. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. Oct 2006.
Pannacciulli N, et al. Higher fasting plasma concentrations of glucagon-like peptide 1 are associated with higher resting energy expenditure and fat oxidation rates in humans. Am. J. Clin. Nutr. Sep 2006; 84(3): 556-560.
Schirra J, et al. Endogenous glucagon-like peptide 1 controls endocrine pancreatic secretion and antro-pyloro-duodenal motility in humans. Gut. Feb 2006; 55(2): 243-251.
|
|
Description |
Glucagon-Like Peptide (GLP)-1, human produced in E. coli is a single, non-glycosylated, polypeptide chain containing 30 amino acids and having a molecular mass of 3,298.7 Da. GLP-1 is a incretion hormone, which has been shown to stimulate secretion of insulin in the presence of elevated blood glucose concentrations, but not during periods of low blood glucose concentrations (hypoglycemia). GLP-1 is an insulinotropic hormone that has the ability to render pancreatic beta cells glucose-competent. It has been used in the therapy of type-2 diabetes. GLP-1 has activities of cyokines, although it is not classified as such due its small size GLP-1 enhances cell differentiation, plays a role in tissue regeneration, and mediates cytoprotection. rHuGLP-1 is purified by proprietary chromatographic techniques. |
Source |
E. coli |
M.W. |
3,298.7 Da |
Purity |
Greater than 95.0% as determined by the following methods:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE |
Endotoxin Level |
Less than 0.1 ng/µg (IEU/µg) of rHuGLP-1 |
Specific Activity |
1. Regulates glucose levels rapidly
2. Reduces insulin resistance
3. Reduces glucagon
4. Reduces HbA1c
5. Stimulates beta cell growth, which in turn stimulates insulin production |
Storage |
Although lyophilized rHuGLP-1 can remain stable at room temperature for three weeks, it is best stored desiccated below -18°C. Upon reconstitution, rHuGLP-1 may be stored at 4°C for up to one week and below -18°C for longer periods.
For long-term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid freeze-thaw cycles. |
Formulation |
The protein (1 mg/ml) is lyophilized after extensive dialyses against 0.1 mg sodium phosphate monobasic and 1.6 mg sodium phosphate dibasic. |
Reconstitution |
It is recommended that the lyophilized rHuGLP-1 be reconstituted in sterile 18 MΩ-cm H2O of not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Quantitation |
Protein quantitation is carried out by two independent methods:
1. UV spectroscopy at 280 nm
2. RP-HPLC analysis, using a calibrated solution of GLP-1 as a reference standard |
Stability |
Lyophilized Glucagon Like Peptide-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GLP-1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Sequence analysis |
The sequence of the first five N-terminal amino acids has been found to be His-Ala-Glu-Gly-Thr. |
Note |
; |
