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Granulocyte Colony Stimulating Factor (G-CSF), human
| Full Name |
Granulocyte Colony Stimulating Factor (G-CSF), Human
|
Abbreviated Name-1 |
rHuG-CSF; G-CSF |
| Documents |
|---|
 Document-MSDS: 15088_20111227200143.PDF (PDF) |
| Document-COA: 15484_20120206004324.PDF (PDF) |
| Figures |
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| Reference |
|---|
Okamoto H, et al. A combination chemotherapy of carboplatin and irinotecan with granulocyte colony-stimulating factor (G-CSF) support in elderly patients with small cell lung cancer. Lung Cancer. Aug 2006; 53(2): 197-203.
Zhang Y, et al. Synergistic effects of granulocyte-colony stimulating factor and macrophage-colony stimulating factor on recovery of donor hematopoietic cells in allogeneic bone marrow transplantation. Oncol. Rep. Aug 2006; 16(2): 367-371.
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Description |
Human Granulocyte Colony Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.
Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18,800 Da.
|
Source |
E. coli |
M.W. |
18,800 Da |
UV Scan |
The maximal absorption wave is 278+/-3 nm. |
Purity |
Greater than 95% as determined by:
(a) Analysis by SEC-HPLC
(b)Analysis by reducing and non-reducing SDS-PAGE silver-stained gel |
Endotoxin Level |
Less than 0.03ng/µg (0.3 IEU/µg) determined by LAL test. |
Specific Activity |
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 6.0×107 IU/mg. |
Storage |
Lyophilized Human Granulocyte Colony Stimulating Factor (G-CSF) remains stable at room temperature for
three weeks, but it is best stored desiccated below -18°C.
Upon reconstitution Human Granulocyte Colony Stimulating Factor (G-CSF) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles. |
Formulation |
The protein was lyophilized after extensive dialysis against 10mM HAc-NaAc, pH4.0, 150mM NaCl, 0.004% Tween 80, 5% Mannitol buffer. |
Reconstitution |
It is recommended to reconstitute the lyophilized rHuG-CSF in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
Quantitation |
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of recombinant G-CSF as a Reference Standard.
|
Sequence analysis |
The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro-Gln-Ser-Phe-Leu-Leu.
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Note |
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