| Full Name |
Interleukin-6 (IL-6), Human
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Abbreviated Name-1 |
rHuIL-6; rHuIL6; Interleukin-6; Interleukin6 |
| Documents |
|---|
 Document-MSDS: 15084_20111227195422.PDF (PDF) |
| Document-COA: 15492_20120206011837.PDF (PDF) |
| Figures |
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| Reference |
|---|
Wernstedt I, et al. Reduced stress- and cold-induced increase in energy expenditure in IL-6 deficient mice. Am. J. Physiol. Regul. Integr. Comp. Physiol.Feb 2006
Itoh S, et al. A critical role for interleukin-6 family-mediated Stat3 activation in osteoblast differentiation and bone formation. Bone. Sep 2006; 39 (3): 505-512.
Pang Y, et al. Role of interleukin-6 in lipopolysaccharide-induced brain injury and behavioral dysfunction in neonatal rats. Neuroscience. Aug 2006; 141 (2): 745-755.
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Description |
Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-β-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1. Human Interleukin-6 (IL-6) produced in E. coli is a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21,000 Da. The Recombinant Human Interleukin-6 (IL-6) is purified by proprietary chromatographic techniques. |
Source |
E. coli |
M.W. |
21,000 Da |
Purity |
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Anion-exchange FPLC
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
Endotoxin Level |
Less than 0.1ng/µg (1 IEU/µg) determined by LAL test. |
Specific Activity |
The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5×107 IU/mg. |
Storage |
Lyophilized Recombinant Human Interleukin-6 (IL-6) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human Interleukin-6 (IL-6) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Formulation |
The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH5.0, 150mM NaAc buffer. |
Reconstitution |
It is recommended to reconstitute the lyophilized rHuIL-6 in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
Quantitation |
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard. |
Sequence |
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM |
Sequence analysis |
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Val-Pro-Pro. |
Note |
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