| Full Name |
Interleukin-15 (IL-15), Human
|
Abbreviated Name-1 |
rHuIL-15; rHuIL15; Interleukin-15; Interleukin15 |
| Documents |
|---|
 Document-COA: 13683_20120206012419.PDF (PDF) |
| Document-MSDS: 15086_20111227195831.PDF (PDF) |
| Figures |
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| Reference |
|---|
Suzuki A, et al. Interleukin-15 increases hepatic regenerative activity. J. Hepatol. Sep 2006; 45 (3): 410-418.
Lin SJ, et al. Effect of interleukin-15 on alloreactivity in umbilical cord blood. Transpl. Immunol. Aug 2006; 16 (2): 112-116.
Waldmann TA. The biology of interleukin-2 and interleukin-15: implications for cancer therapy and vaccine design. Nat. Rev. Immunol. Aug 2006; 6 (8): 595-601.
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Description |
Interleukin 15 (IL-15) is a cytokine that possesses a variety of biological functions. Interleukin 15 (IL-15) stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R. Recombinant Human Interleukin 15 (IL-15) produced in E. coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12,774 Da. |
Source |
E. coli |
M.W. |
12,774 Da |
Purity |
Greater than 98.0% as determined by:
(a)Analysis by RP-HPLC
(b)Anion-exchange FPLC
(c)Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
Endotoxin Level |
Less than 0.1 ng/µg (1 IEU/µg) of rHuIL-15 |
Specific Activity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be <0.5 ng/ml, corresponding to a Specific Activity of 2x106 IU/mg. |
Storage |
Lyophilized Recombinant Human Interleukin 15 (IL-15) remains stable at room temperature for
three weeks, but it is best stored desiccated below -18°C.
Upon reconstitution Recombinant Human Interleukin 15 (IL-15) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles. |
Formulation |
The protein was lyophilized after extensive dialysis against 20mM PB, pH7.5, 150mM NaCl buffer. |
Reconstitution |
It is recommended to reconstitute the lyophilized rHuIL-15 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Quantitation |
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard. |
Sequence |
MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISHESGDTDI HDTVENLIIL ANNILSSNGN ITESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS |
Sequence analysis |
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Trp-Val-Asn-Val.
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Note |
; |
