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Pegylated Granulocyte Colony Stimulating Factor (PEG-G-CSF), human
| Full Name |
Pegylated Granulocyte Colony Stimulating Factor (PEG-G-CSF), Human
|
Abbreviated Name-1 |
rHuPEG-G-CSF; PEG-G-CSF |
| Documents |
|---|
 Document-MSDS: 15098_20111227202220.PDF (PDF) |
| Document-COA: 15494_20120206012807.PDF (PDF) |
| Figures |
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| Reference |
|---|
de Haan G, et al. Efficient mobilization of haematopoietic progenitors after a single injection of pegylated recombinant human granulocyte colony-stimulating factor in mouse strains with distinct marrow-cell pool sizes. Br. J. Haematol. Sep 2000; 110(3): 638-646.
Sainathan SK, et al. PEGylated murine Granulocyte-macrophage colony-stimulating factor: production, purification, and characterization. Protein Expr. Purif. Dec 2005; 44(2): 94-103.
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Description |
Human pegylated granulocyte colony stimulating factor (PEG-GCSF) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18,800 Da. pegylated granulocyte colony stimulating factor (PEG-GCSF) is manufactured by attaching a 20,000 Da methoxypolyethylene glycol propionaldehyde (mPEG-ALD) to the N-terminal amino acid of G-CSF having a total molecular mass of 38,800 Da. |
Source |
E. coli |
M.W. |
20,000 Da |
Purity |
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel. |
Endotoxin Level |
Less than 0.1 ng/µg (1 IEU/µg) of rHuPEG-G-CSF. |
Specific Activity |
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml, corresponding to a Specific Activity of 1×107 IU/mg. |
Storage |
Recombinant Human pegylated granulocyte colony stimulating factor (PEG-GCSF) although stable at 15°C for 1 week should be stored between 2°C-8°C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA). |
Formulation |
The protein was lyophilized after extensive dialysis against 10mM HAc-NaAc, pH4.0, 150mM NaCl, 0.004% Tween 80, 5% Mannitol buffer. |
Quantitation |
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction
coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE
computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of PEG-G-CSF as a Reference Standard.
|
Sequence analysis |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly. |
Note |
; |
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