Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase

Full Name

Abbreviated Name-1

M-MLV RT;

Documents
Document-MSDS: 7551_20081030012707.PDF (PDF)
Document-COA: 10491_20090819021843.PDF (PDF)
Figures
Reference

Description

GenScript Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand. The enzyme lacks 3â-5â exonuclease activity.

Source

E. coli

Purity

Purity is > 90% as determined by SDS-PAGE.

Specific Activity

One unit is defined as the amount of enzyme required to catalyze the transfer of 1 nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C.
The reaction conditions are as follows: 50 mM Tris-HCl (pH 8.3), 7 mM MgCl₂, 40 mM KCl, 10 mM DTT, 0.1 mg/ml BSA, 0.5 mM [³H]dTTP, 0.025 mM oligo (dT), 0.25 mM poly(A), and 0.01% NP-40.

Storage

Store the protein at -20°C. Avoid exposure to frequent temperature changes.

Concentration

200,000 units/ml in 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 1 mM DTT, 0.01% NP-40, 0.1 mM EDTA, 50% glycerol

Note

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