| Full Name |
Interleukin-17 (IL-17), Human
|
Abbreviated Name-1 |
Interleukin-17; Interleukin17; IL17; |
|
Description |
The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 132 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells. GenScript Interleukin (IL)-17, human is a 31.0 kDa disulfide-linked homodimer of two 136 amino acid polypeptide chains. |
Source |
E. coli |
M.W. |
15,000 Da |
Purity |
Greater than 95% by SDS-PAGE analyses |
Endotoxin Level |
Less than 1 EU/µg as determined by LAL method |
Specific Activity |
The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line was found to be approximately 2 ng/ml. |
Storage |
Lyophilized IL-17 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-17 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA oor BSA). |
Formulation |
Sterile filtered lyophilized (freeze-dried) powder, with 20 mM Citrate, 0.1 M NaCl, pH 4.5 |
Reconstitution |
It is recommended to reconstitute the lyophilized IL-17 in sterile 18 MΩ-cm H2O no less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Stability |
The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
Reconstituted human IL-17 should be stored in working aliquots at -20°C.
Avoid repeated freeze-thaw cycles.
|
Applications |
FOR RESEARCH USE ONLY. NOT FOR HUMAN USE. |
Sequence analysis |
The sequence of the first 5 N-terminal amino acids was determined and was found to be Met-Gly-Ile-Thr-Ile. |
Note |
; |
Document-MSDS: 15120_20111229222736.PDF (PDF)