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Interleukin-22 (IL-22), mouse
| Full Name |
Interleukin-22 (IL-22), Mouse
|
Abbreviated Name-1 |
IL-22 (Mouse), rmIL-22; |
|
Description |
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse Interleukin-22 (IL-22) cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. Interleukin-22 (IL-22) has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. Interleukin-22 (IL-22) is produced by normal mouse T cells upon Con activation. Mouse Interleukin-22 (IL-22) expression is also induced in various organs upon lipopolysaccharide injection, suggesting that Interleukin-22 (IL-22) may be involved in inflammatory responses. The functional Interleukin-22 (IL-22) receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4), belonging to the class II cytokine receptor family.
Mouse Interleukin-22 (IL-22), produced in E. coli, is a single, non-glycosylated polypeptide chain containing 147 amino acids and having a molecular mass of 16,700 Da. |
Source |
E. coli |
M.W. |
16,700 Da |
Purity |
The purity of GenScript Recombinant Mouse IL-22 is greater than 97.0%, as determined by the following methods:
(a) RP-HPLC analysis
(b) Reducing and non-reducing SDS-PAGE silver stained gel analysis |
Endotoxin Level |
The endotoxin level of GenScript Recombinant Mouse IL-22 is below 0.1 ng/µg (1 IEU/µg), as determined by LAL test. |
Storage |
Lyophilized Mouse Interleukin-22 (IL-22) remains stable at room temperature for
three weeks, but it is best stored desiccated below -18°C.
Upon reconstitution Recombinant Mouse Interleukin-22 (IL-22) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles. |
Formulation |
The protein was lyophilized after extensive dialysis against 20mM PB, pH 6.5, 300mM NaCl buffer. |
Reconstitution |
It is recommended that reconstitute the lyophilized rmIL-22 be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Specificity |
GenScript Recombinant Mouse IL-22 is fully biologically active relative to standard. The ED50, as determined by its ability to induce IL-10 secretion in Colo205 cells, is less than 0.5 ng/ml, corresponding to a specific activity of 2.0×106 IU/mg. |
Sequence |
The sequence of the first five N-terminal amino acids has been found to be Met-Leu-Pro-Val-Asn. |
Note |
; |
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Document-MSDS: 15072_20111227042104.PDF (PDF)