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Cat. No.	Name	Size	Price
Z02522-5	AMPK (α2/β2/γ1), Active (Mammalian Expression System)	5 μg	$495
Z02522-50	AMPK (α2/β2/γ1), Active (Mammalian Expression System)	50 μg	$2970

AMPK (α2/β2/γ1), Active (Mammalian Expression System)

Full Name

Abbreviated Name-1

AMPK221, AMPK (A2/B2/G1);

Documents
Document-COA: 11197_20091108220043.PDF (PDF)
Document-MSDS: 11201_20091108221301.PDF (PDF)
Document-COA: 14502_20111114040053.PDF (PDF)
Figures
Reference

Description

Recombinant human trimeric protein complex composed of full length His-tagged PRKAA2, His-tagged PRKAB2 and Flag-tagged PRKAG1, co-expressed in HEK293. The complex was activated in vitro with histidine-tagged CAMKK1.

Source

Recombinant enzyme co-expressed in HEK293

Purity

>80% as estimated by a Coomassie blue-stained SDS-PAGE gel

Specific Activity

1664 nmole of phosphate transferred to SAMS peptide (HMRSAMSGLHLVKRR) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.107 μg/ml. Activated 2.3 times by 100 μM AMP.

Storage

Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws. If properly stored at -80°C, this product is guaranteed for 6 months from date of purchase.

Concentration

0.500 mg/ml, as determined by Bradford protein assay with BSA as a standard.

Applications

Kinase Assay, Western Blot

Note

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