Case Study—Protein with Biological Activity
Production and purification of proteins with biological activity is essential in drug discovery. Multifunctional units at GenScript secure successful delivery of bioactive proteins based on our state-of-the-art equipment and methodologies. A case study below shows groups of protein production and assay development teaming up deliver
enzyme with qualified specific activity, higher than customer expected. After modifying the method of cell-breaking, we increase the final yield of protein of interest with purity of above 90%.
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Fig. A: SDS-PAGE analysis of the fractions upon the IEX chromatography
Lane 1-47: Gradient elution fractions
Lane M: Smart Broad-Range Protein Standard (GenScript, Cat. No. MM0906)
Lane S: The supernatant after homogenizer with high pressure
Lane ft: Flow through
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Fig. B: Enzyme assay monitoring the fractionation by DEAD Sepharose
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Fig. C: Fractions pooled based on activity analysis for further ADP Sepharose affinity purification
Fig. D: SDS-PAGE analysis of the purified protein
Lane M: Smart Broad-Range Protein Standard (GenScript, Cat. No. MM0906)
Lane S: The supernatant after homogenizer with high pressure
Lane ft: Flow through
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