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Plasmidic siRNA Expression Vectors

These vectors are designed for mammalian transfection. They each carry a neomycin, hygromycin, or zeomycin resistance gene as a selectable marker, which can be used for establishing stable cell lines. The pRNAT vectors also carry a GFP marker (coral GFP or cGFP) under CMV promoter* control, which can be used to track transfection efficiency. They use regular or enhanced U6, or H1, or CMV promoters to drive siRNA expression.

Cat. No.
Vector
Promoter
Resistance
Marker
Map
Price
Selected
SD1201
pRNA-U6.1/Neo
U6
Neomycin
-
Map
$225
SD1202
pRNA-U6.1/Hygro
U6
Hygromycin
-
Map
$225
SD1207
pRNA-U6.1/Zeo
U6
Zeomycin
-
Map
$225
SD1203
pRNA-H1.1/Neo
H1
Neomycin
-
Map
$225
SD1204
pRNA-H1.1/Hygro
H1
Hygromycin
-
Map
$225
SD1208
pRNA-H1.1/Zeo
H1
Zeomycin
-
Map
$225
SD1231
pRNA-CMV3.1/Neo
CMV
Neomycin
-
Map
$225
SD1232
pRNA-CMV3.1/Hygro
CMV
Hygromycin
-
Map
$225
SD1233
pRNA-CMV3.1/Puro
CMV
Puromycin
-
Map
$225
SD1211
pRNAT-U6.1/Neo
U6
Neomycin
cGFP
Map
$350
SD1212
pRNAT-U6.1/Hygro
U6
Hygromycin
cGFP
Map
$350
SD1213
pRNAT-H1.1/Neo
H1
Neomycin
cGFP
Map
$350
SD1214
pRNAT-H1.1/Hygro
H1
Hygromycin
cGFP
Map
$350
SD1261
pRNAT-CMV3.1/Neo
CMV
Neomycin
cGFP
Map
$350
SD1262
pRNAT-CMV3.1/Hygro
CMV
Hygromycin
cGFP
Map
$350
SD1263
pRNAT-CMV3.1/Puro
CMV
Puromycin
cGFP
Map
$350
SD1264
pRNAT-CMV3.2/Neo
CMV
Neomycin
cGFP
Map
$350
SD1265
pRNAT-CMV3.2/Hygro
CMV
Hygromycin
cGFP
Map
$350
SD1266
pRNAT-CMV3.2/Puro
CMV
Puromycin
cGFP
Map
$350
SD1550
pRNA-U6.3/Neo
U6 (enhanced)
Neomycin
-
Map
$225
SD1552
pRNA-H1.3/Neo
H1 (enhanced)
Neomycin
-
Map
$225
SD1561
pRNAT-U6.3/Hygro
U6 (enhanced)
Hygromycin
cGFP
Map
$350
SD1563
pRNAT-H1.3/Hygro
H1 (enhanced)
Hygromycin
cGFP
Map
$350

To optimize the experimental parameters of DNA vector-based siRNA constructs, we have designed positive controls, which can knock down firefly luciferase and Renilla luciferase. These can be used for initial test experiments.

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Inducible siRNA Expression Vectors

The H1.2 promoter is an engineered inducible promoter** containing a tetracycline operator (TetO1). The tetracycline operator itself has no effect on expression. When the tetracycline repressor (TetR) is present, it effectively binds the TetO1 and blocks the transcription. In the presence of tetracycline or doxycycline, the inducer binds TetR, causing the TetR protein to release the TetO1 site, derepressing transcription.

pRNATin-H1.2 is designed for mammalian transfection. Each vector carries a neomycin or hygromycin resistance gene, which can be used to establish stable cell lines, and a coral GFP marker under CMV promoter* control, which can be used to track transfection efficiency. Adenoviral, retroviral, and lentiviral expression vectors are also available for this inducible system.

Cat. No.
Vector
Promoter
Resistance
Marker
Map
Price
Selected
SD1223
H1
Neo
cGFP
Map
$350
SD1224
H1
Hygro
cGFP
Map
$350
SD1220
H1
Neo
-
Map
$350
SD1229
H1
Kanamycin
cGFP
Map
$350
SD1234
H1
Neomycin
-
Map
$350
SD1254
H1 (inducible)
Hygro
cGFP
Map
$350
SD1260
H1 (inducible)
Neo
cGFP
Map
$350

We have designed a positive control for this inducible expression vector, SD1523 (pRNATin-H1.2/Neo/siFLuc), that can knock down firefly luciferase.

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Viral siRNA Expression Vectors

Adenoviral siRNA Shuttle Vectors:

These adenoviral shuttle vectors are compatible with the Clontech Adeno-XTM Expression System. Each uses either a U6 or H1 promoter to drive siRNA expression.

Cat. No.
Vector
Promoter
Resistance
Marker
Map
Price
Selected
SD1205
U6
Neo
-
Map
$225
SD1206
H1
Neo
-
Map
$225
SD1216
H1
Neo
cGFP
Map
$350
SD1234
H1 (inducible)
Neo
-
Map
$350

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Adenoviral siRNA Vectors:

The following GenScript adenoviral vectors are compatible with the Stratagene AdEasyTM Adenoviral Vector System. Each uses either an H1 or inducible H1 promoter to drive siRNA expression. The pRNAT vectors carry a GFP marker (coral GFP or cGFP) under CMV promoter* control, which can be used to track transfection efficiency.

Cat. No.
Vector
Promoter
Resistance
Marker
Map
Price
Selected
SD1209
H1
Kanamycin
-
Map
$225
SD1219
H1
Kanamycin
cGFP
Map
$350
SD1229
H1 (inducible)
Kanamycin
cGFP
Map
$350

 

Lentiviral siRNA Vectors:

GenScript Lentiviral siRNA expression vectors are compatible with the Invitrogen ViraPowerTM Lentiviral Expression System. They are designed for siRNA expression in both dividing and nondividing cells. Each vector contains a neomycin resistance gene for establishing stable cell lines and a coral GFP gene for tracking transfection efficiency, respectively driven by an SV40 promoter and a CMV promoter. The vector uses a Rous sarcoma virus (RSV) enhancer/promoter fused to HIV 5'LTR (PRSV/5'LTR) for viral transcription and HIV 3'LTR for packaging.

Cat. No.
Vector
Promoter
Resistance
Marker
Map
Price
Selected
SD1259
U6
Neo
cGFP
Map
$350
SD1260
H1 (inducible)
Neo
cGFP
Map
$350

 

Retroviral siRNA Vectors:

GenScript Retroviral siRNA expression vectors are compatible with the Clontech Retro-XTM Expression System. The siRNA insert is cloned between the Mli and Xho sites. An H1 promoter drives siRNA expression. The vector contains a hygromycin resistance gene under the control of a CMV promoter* for establishing stable cell lines. The vector uses MSV 3'LTR for viral transcription and a CMV enhancer/promoter joined to MSV 5'LTR (CMV/MSV 5'LTR) for packaging.

Cat. No.
Vector
Promoter
Resistance
Marker
Map
Price
Selected
SD1241
H1
Hygro
-
Map
$225
SD1253
H1
Hygro
cGFP
Map
$350
SD1254
H1 (inducible)
Hygro
cGFP
Map
$350

References:

 

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* Limited Use Label License: The use of CMV promoter is covered under U. S. Patents Nos. 5,168,062 and 5,385,839, owned and licensed by the University of Iowa Research Foundation and is sold for research use only. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation (UIRF), 214 Technology Innovation Center, Iowa City, Iowa 52242. For further information, please contact the Associate Director of UIRF at 319-335-4546.

** Limited Use Label License: This H1.2 inducible promoter is covered by United States Patent Applications Nos. 60,505,677, owned by GenScript Corporation. The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) under these patent rights to perform the siRNA synthesis methods claimed in the patent application for research and development purposes solely in conjunction with this product.