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Plasmidic siRNA Expression Vectors

These vectors are designed for mammalian transfection. They each carry a neomycin, hygromycin, or zeomycin resistance gene as a selectable marker, which can be used for establishing stable cell lines. The pRNAT vectors also carry a GFP marker (coral GFP or cGFP) under CMV promoter* control, which can be used to track transfection efficiency. They use regular or enhanced U6, or H1, or CMV promoters to drive siRNA expression.

Cat. No.	Vector	Promoter	Resistance	Marker	Map	Price
SD1201	pRNA-U6.1/Neo	U6	Neomycin	-	Map	$225
SD1202	pRNA-U6.1/Hygro	U6	Hygromycin	-	Map	$225
SD1207	pRNA-U6.1/Zeo	U6	Zeomycin	-	Map	$225
SD1203	pRNA-H1.1/Neo	H1	Neomycin	-	Map	$225
SD1204	pRNA-H1.1/Hygro	H1	Hygromycin	-	Map	$225
SD1208	pRNA-H1.1/Zeo	H1	Zeomycin	-	Map	$225
SD1231	pRNA-CMV3.1/Neo	CMV	Neomycin	-	Map	$225
SD1232	pRNA-CMV3.1/Hygro	CMV	Hygromycin	-	Map	$225
SD1233	pRNA-CMV3.1/Puro	CMV	Puromycin	-	Map	$225
SD1211	pRNAT-U6.1/Neo	U6	Neomycin	cGFP	Map	$350
SD1212	pRNAT-U6.1/Hygro	U6	Hygromycin	cGFP	Map	$350
SD1213	pRNAT-H1.1/Neo	H1	Neomycin	cGFP	Map	$350
SD1214	pRNAT-H1.1/Hygro	H1	Hygromycin	cGFP	Map	$350
SD1261	pRNAT-CMV3.1/Neo	CMV	Neomycin	cGFP	Map	$350
SD1262	pRNAT-CMV3.1/Hygro	CMV	Hygromycin	cGFP	Map	$350
SD1263	pRNAT-CMV3.1/Puro	CMV	Puromycin	cGFP	Map	$350
SD1264	pRNAT-CMV3.2/Neo	CMV	Neomycin	cGFP	Map	$350
SD1265	pRNAT-CMV3.2/Hygro	CMV	Hygromycin	cGFP	Map	$350
SD1266	pRNAT-CMV3.2/Puro	CMV	Puromycin	cGFP	Map	$350
SD1550	pRNA-U6.3/Neo	U6 (enhanced)	Neomycin	-	Map	$225
SD1552	pRNA-H1.3/Neo	H1 (enhanced)	Neomycin	-	Map	$225
SD1561	pRNAT-U6.3/Hygro	U6 (enhanced)	Hygromycin	cGFP	Map	$350
SD1563	pRNAT-H1.3/Hygro	H1 (enhanced)	Hygromycin	cGFP	Map	$350

To optimize the experimental parameters of DNA vector-based siRNA constructs, we have designed positive controls, which can knock down firefly luciferase and Renilla luciferase. These can be used for initial test experiments.

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Inducible siRNA Expression Vectors

The H1.2 promoter is an engineered inducible promoter** containing a tetracycline operator (TetO1). The tetracycline operator itself has no effect on expression. When the tetracycline repressor (TetR) is present, it effectively binds the TetO1 and blocks the transcription. In the presence of tetracycline or doxycycline, the inducer binds TetR, causing the TetR protein to release the TetO1 site, derepressing transcription.

pRNATin-H1.2 is designed for mammalian transfection. Each vector carries a neomycin or hygromycin resistance gene, which can be used to establish stable cell lines, and a coral GFP marker under CMV promoter* control, which can be used to track transfection efficiency. Adenoviral, retroviral, and lentiviral expression vectors are also available for this inducible system.

Cat. No.	Vector	Promoter	Resistance	Marker	Map	Price
SD1223	pRNATin-H1.2/Neo	H1	Neo	cGFP	Map	$350
SD1224	pRNATin-H1.2/Hygro	H1	Hygro	cGFP	Map	$350
SD1220	pRNAin-H1.2/Neo	H1	Neo	-	Map	$350
SD1229	pRNATin-H1.2/Adeno	H1	Kanamycin	cGFP	Map	$350
SD1234	pRNAin-H1.2/Shuttle	H1	Neomycin	-	Map	$350
SD1254	pRNATin-H1.4/Retro	H1 (inducible)	Hygro	cGFP	Map	$350
SD1260	pRNATin-H1.4/Lenti	H1 (inducible)	Neo	cGFP	Map	$350

We have designed a positive control for this inducible expression vector, SD1523 (pRNATin-H1.2/Neo/siFLuc), that can knock down firefly luciferase.

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Viral siRNA Expression Vectors

Adenoviral siRNA Shuttle Vectors:

These adenoviral shuttle vectors are compatible with the Clontech Adeno-X^TM Expression System. Each uses either a U6 or H1 promoter to drive siRNA expression.

Cat. No.	Vector	Promoter	Resistance	Marker	Map	Price
SD1205	pRNA-U6.1/Shuttle	U6	Neo	-	Map	$225
SD1206	pRNA-H1.1/Shuttle	H1	Neo	-	Map	$225
SD1216	pRNAT-H1.1/Shuttle	H1	Neo	cGFP	Map	$350
SD1234	pRNA-H1.2/Shuttle	H1 (inducible)	Neo	-	Map	$350

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Adenoviral siRNA Vectors:

The following GenScript adenoviral vectors are compatible with the Stratagene AdEasy^TM Adenoviral Vector System. Each uses either an H1 or inducible H1 promoter to drive siRNA expression. The pRNAT vectors carry a GFP marker (coral GFP or cGFP) under CMV promoter* control, which can be used to track transfection efficiency.

Cat. No.	Vector	Promoter	Resistance	Marker	Map	Price
SD1209	pRNA-H1.1/Adeno	H1	Kanamycin	-	Map	$225
SD1219	pRNAT-H1.1/Adeno	H1	Kanamycin	cGFP	Map	$350
SD1229	pRNATin-H1.2/Adeno	H1 (inducible)	Kanamycin	cGFP	Map	$350

Lentiviral siRNA Vectors:

GenScript Lentiviral siRNA expression vectors are compatible with the Invitrogen ViraPower^TM Lentiviral Expression System. They are designed for siRNA expression in both dividing and nondividing cells. Each vector contains a neomycin resistance gene for establishing stable cell lines and a coral GFP gene for tracking transfection efficiency, respectively driven by an SV40 promoter and a CMV promoter. The vector uses a Rous sarcoma virus (RSV) enhancer/promoter fused to HIV 5'LTR (PRSV/5'LTR) for viral transcription and HIV 3'LTR for packaging.

Cat. No.	Vector	Promoter	Resistance	Marker	Map	Price	Selected
SD1259	pRNAT-U6.2/Lenti	U6	Neo	cGFP	Map	$350
SD1260	pRNATin-H1.4/Lenti	H1 (inducible)	Neo	cGFP	Map	$350

Retroviral siRNA Vectors:

GenScript Retroviral siRNA expression vectors are compatible with the Clontech Retro-X^TM Expression System. The siRNA insert is cloned between the Mli and Xho sites. An H1 promoter drives siRNA expression. The vector contains a hygromycin resistance gene under the control of a CMV promoter* for establishing stable cell lines. The vector uses MSV 3'LTR for viral transcription and a CMV enhancer/promoter joined to MSV 5'LTR (CMV/MSV 5'LTR) for packaging.

Cat. No.	Vector	Promoter	Resistance	Marker	Map	Price
SD1241	pRNA-H1.1/Retro	H1	Hygro	-	Map	$225
SD1253	pRNAT-H1.4/Retro	H1	Hygro	cGFP	Map	$350
SD1254	pRNATin-H1.4/Retro	H1 (inducible)	Hygro	cGFP	Map	$350

References:

Here is a list of references, articles, and other projects that cite the use of GenScript siRNA vectors.