| Primer Walking
Primer walk sequencing is the method of choice for sequencing DNA fragments of between 1.3 and 7 kb, and it is applicable to PCR amplicons, plasmid inserts, or even entire plasmids. GenScript's primer walk sequencing service is divided into single-strand primer walks and double-strand primer walks according to the customers' requirements. Our service package includes the following:
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Primer design and synthesis |
Sequencing reactions |
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Sequencing |
Dye terminator removal |
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Primer design and synthesis |
DNA sequence finishing and assembly |
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Sequencing data delivery/retrieval via secure web server |
FDA-Quality DNA Sequencing
GenScript performs all FDA submission services in a fully compliant laboratory environment that exceeds the FDA's standards for quality, reliability, and consistency. Our service guarantees the following:
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Detailed sequencing strategy
Minimum fourfold repeats |
Complete double-stranded coverage
Absolutely confidentiality |
Our final delivery package includes the original electropherograms, consensus sequence, and QC reports. Upon request, we can provide sequence discrepancy reports comparing observed vs expected sequences and any effects of insertions or deletions on amino acid sequence.
Large-Scale Sequencing
GenScript has many years of experience in large-scale sequencing. Our large-scale sequencing service is de novo, which does not require any pre-existing information about the sequence. Our large-scale DNA sequencing service package includes the following:
- High-throughput single- and double-stranded sequencing
- High-throughput DNA extraction/purification (96- or 384-well format)
- Data delivery via our secure web server
- Library sequencing (BAC, PAC, Cosmid, EST)
- DNA sequence finishing and assembly
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The final delivery package includes the original electropherograms, consensus sequence, and QC reports.
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Online Order
Online Quotation
