Eef2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Eef2
Entrez Gene ID	13629
Full Name	eukaryotic translation elongation factor 2
Synonyms	Ef-2
General protein information	Preferred Names eukaryotic translation elongation factor 2 Names elongation factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA Protein Name

NM_007907.2 NP_031933.1 elongation factor 2
Pathways from BioSystems

mRNA	Protein	Name
NM_007907.2	NP_031933.1	elongation factor 2

Homologies

Pan troglodytes (chimpanzee)	LOC100614367	XP_003316060.2
	EEF2	XP_001118006.2
Drosophila melanogaster (fruit fly)	Ef2b	NP_525105.2
Mus musculus (house mouse)	Eef2	NP_031933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009441	XP_307854.2
Danio rerio (zebrafish)	eef2b	NP_956752.2
Arabidopsis thaliana (thale cress)	LOS1	NP_849818.1
Oryza sativa (rice)	Os01g0742200	NP_001044211.1
Bos taurus (cattle)	EEF2	NP_001068589.1
Gallus gallus (chicken)	EEF2	NP_990699.1
Caenorhabditis elegans (roundworm)	eef-2	NP_001251010.1
Homo sapiens (human)	EEF2	NP_001952.1
Canis lupus familiaris (dog)	EEF2	XP_533949.3
Rattus norvegicus (Norway rat)	Eef2	NP_058941.1
Xenopus tropicalis (tropical clawed frog)	eef2.1	NP_989255.1

Gene Ontology
Bibliography

Related articles in PubMed

Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease.
Fakruddin M, Wei FY, Suzuki T, Asano K, Kaieda T, Omori A, Izumi R, Fujimura A, Kaitsuka T, Miyata K, Araki K, Oike Y, Scorrano L, Suzuki T, Tomizawa K
Cell reports22(2)482-496(2018 Jan)

Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis.
Deng Z, Luo P, Lai W, Song T, Peng J, Wei HK
Biochemical and biophysical research communications494(1-2)278-284(2017 12)

Solid tumor therapy by selectively targeting stromal endothelial cells.
Liu S, Liu J, Ma Q, Cao L, Fattah RJ, Yu Z, Bugge TH, Finkel T, Leppla SH
Proceedings of the National Academy of Sciences of the United States of America113(28)E4079-87(2016 07)

Temporally defined neocortical translation and polysome assembly are determined by the RNA-binding protein Hu antigen R.
Kraushar ML, Thompson K, Wijeratne HR, Viljetic B, Sakers K, Marson JW, Kontoyiannis DL, Buyske S, Hart RP, Rasin MR
Proceedings of the National Academy of Sciences of the United States of America111(36)E3815-24(2014 Sep)

IL-6 impairs myogenic differentiation by downmodulation of p90RSK/eEF2 and mTOR/p70S6K axes, without affecting AKT activity.
Pelosi M, De Rossi M, Barberi L, Musar? A
BioMed research international2014206026(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis.
Title: Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis.

IL-6 induces the activation of the Stat3 signaling and promotes the downmodulation of the p90RSK/eEF2 and mTOR/p70S6K axes, while it does not affect the activation of AKT.
Title: IL-6 impairs myogenic differentiation by downmodulation of p90RSK/eEF2 and mTOR/p70S6K axes, without affecting AKT activity.

AMPKalpha2 controls cardiac p70S6K under normoxia and regulates eEF-2 but not the mTOR-p70S6K pathway during ischemia.
Title: Differential regulation of eEF2 and p70S6K by AMPKalpha2 in heart.

The MKK3/6-p38gamma/delta pathway mediated an inhibitory phosphorylation of eukaryotic elongation factor 2 (eEF2) kinase, which in turn promoted eEF2 activation (dephosphorylation) and subsequent TNF-alpha elongation.
Title: Eukaryotic elongation factor 2 controls TNF-? translation in LPS-induced hepatitis.

Thiopental inhibits global protein synthesis by repression of eukaryotic elongation factor 2 and protects from hypoxic neuronal cell death.
Title: Thiopental inhibits global protein synthesis by repression of eukaryotic elongation factor 2 and protects from hypoxic neuronal cell death.

The following Eef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22573	NM_007907.2 Latest version!	Mus musculus eukaryotic translation elongation factor 2 (Eef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$503.30 $719.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eef2 ( NM_007907.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031933.1 Mus musculus eukaryotic translation elongation factor 2 (Eef2), mRNA.

Price & Availability↑

CloneID	OMu22573
Clone ID Related Accession (Same CDS sequence)	NM_007907.2
Accession Version	NM_007907.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	elongation factor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ170931.1, AK168794.1 and CJ316208.1. On May 21, 2009 this sequence version replaced NM_007907.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC007152.1, AK159421.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAACT TCACAGTAGA TCAGATCCGT GCCATCATGG ACAAGAAAGC CAACATCCGG 
AACATGTCAG TCATCGCCCA TGTGGACCAC GGCAAGTCCA CGCTGACCGA CTCCCTTGTG 
TGCAAGGCTG GCATCATTGC CTCTGCCCGA GCTGGGGAGA CGCGCTTCAC TGACACTCGC 
AAGGATGAGC AGGAGCGCTG CATCACAATC AAATCCACCG CCATCTCCCT CTTCTACGAG 
CTCTCTGAGA ACGACCTGAA CTTCATTAAG CAGAGCAAGG ATGGCTCGGG CTTCCTCATC 
AACCTCATCG ACTCTCCAGG CCATGTGGAC TTCTCTTCAG AGGTGACAGC TGCCTTGCGT 
GTCACCGATG GAGCTCTGGT GGTGGTGGAC TGTGTGTCTG GCGTGTGTGT GCAGACAGAA 
ACTGTGCTGC GCCAGGCCAT CGCTGAACGC ATCAAGCCCG TCCTGATGAT GAACAAGATG 
GACCGGGCCC TGCTGGAGCT GCAGCTGGAG CCCGAGGAGC TCTACCAGAC CTTCCAGCGC 
ATCGTGGAGA ACGTCAACGT CATCATCTCT ACCTACGGCG AGGGCGAGAG TGGGCCCATG 
GGCAATATCA TGATTGACCC CGTCCTGGGC ACCGTAGGCT TTGGTTCTGG CCTGCATGGC 
TGGGCCTTCA CCCTGAAGCA GTTTGCGGAG ATGTATGTGG CCAAGTTTGC AGCCAAGGGT 
GAGGGCCAGC TGAGCGCAGC CGAGCGTGCC AAGAAAGTAG AGGACATGAT GAAGAAGCTG 
TGGGGAGACC GGTACTTTGA TCCGGCCAAT GGCAAGTTCA GTAAGTCAGC CAATAGCCCA 
GATGGGAAAA AACTTCCCCG CACCTTCTGC CAGCTCATCC TGGACCCCAT CTTCAAGGTG 
TTCGACGCCA TCATGAACTT CAGGAAGGAG GAGACAGCCA AGCTGATCGA GAAGCTGGAC 
ATCAAGCTGG ACAGCGAGGA CAAAGACAAG GAGGGCAAGC CATTGCTCAA GGCTGTGATG 
CGCCGCTGGC TGCCTGCAGG GGACGCCCTC CTGCAGATGA TCACCATCCA CTTACCATCC 
CCCGTCACTG CACAGAAGTA CCGTTGTGAG CTGCTATACG AGGGGCCTCC CGACGATGAG 
GCCGCCATGG GTATTAAGAG CTGCGACCCC AAAGGCCCAC TTATGATGTA CATTTCCAAG 
ATGGTGCCAA CCTCTGACAA AGGCCGCTTC TATGCCTTTG GTAGAGTGTT CTCTGGGGTG 
GTGTCCACGG GCCTGAAGGT CCGGATCATG GGCCCCAACT ACACGCCTGG GAAGAAAGAG 
GACCTATACC TGAAGCCTAT CCAGAGAACC ATTCTGATGA TGGGCCGCTA CGTGGAGCCG 
ATTGAGGACG TGCCATGTGG AAACATTGTG GGGCTGGTCG GAGTGGACCA GTTCCTTGTG 
AAGACGGGGA CCATCACTAC CTTTGAGCAC GCTCACAACA TGCGCGTGAT GAAGTTCAGC 
GTCAGCCCTG TCGTCCGTGT GGCAGTGGAG GCCAAGAACC CAGCTGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGGCT GGCTAAGTCT GACCCTATGG TGCAGTGCAT CATTGAGGAG 
TCTGGAGAAC ATATTATTGC TGGCGCCGGT GAGCTGCACC TGGAGATCTG CCTTAAGGAC 
CTGGAGGAGG ACCATGCCTG CATCCCCATC AAGAAATCTG ACCCTGTTGT GTCATATCGG 
GAGACAGTCA GTGAGGAATC CAACGTGCTG TGTCTGTCCA AGTCCCCCAA TAAGCACAAC 
CGGCTGTACA TGAAGGCCAG GCCCTTCCCT GATGGCCTGG CAGAGGACAT CGATAAGGGT 
GAGGTGTCTG CCCGCCAGGA GCTCAAGGCA CGTGCCCGCT ACCTGGCCGA AAAGTATGAG 
TGGGACGTTG CTGAAGCCCG CAAGATCTGG TGCTTTGGCC CTGATGGCAC TGGCCCCAAC 
ATTCTCACCG ACATCACCAA GGGTGTGCAG TACCTGAATG AGATCAAGGA CAGTGTGGTG 
GCTGGCTTCC AGTGGGCTAC TAAGGAGGGC GCTCTCTGTG AGGAAAACAT GCGTGGTGTG 
CGGTTTGATG TTCATGATGT GACCCTGCAT GCTGATGCCA TTCACCGGGG AGGTGGCCAG 
ATCATCCCCA CAGCACGCCG CTGCCTGTAT GCCAGTGTGC TGACCGCACA GCCCCGCCTC 
ATGGAGCCTA TCTATCTGGT GGAGATCCAG TGTCCTGAGC AAGTGGTGGG TGGCATCTAC 
GGTGTCCTGA ACAGGAAGCG TGGCCATGTG TTTGAGGAGT CCCAGGTGGC TGGTACCCCC 
ATGTTTGTGG TCAAGGCATA CCTGCCTGTC AATGAGTCCT TTGGCTTCAC CGCTGATCTG 
CGATCCAACA CCGGCGGCCA GGCCTTCCCC CAGTGCGTGT TTGACCACTG GCAGATCCTG 
CCTGGGGATC CTTTTGACAA CAGCAGCCGC CCCAGCCAAG TGGTAGCTGA GACGCGCAAG 
CGCAAGGGCC TGAAAGAGGG CATCCCAGCG CTGGACAACT TCCTGGACAA ACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031933.1
CDS	97..2673
Translation	MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLSAAERAKKVEDMMKKLWGDRYFDPANGKFSKSANSPDGKKLPRTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGVVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKARARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

Target ORF information:

RefSeq Version	NM_007907.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus eukaryotic translation elongation factor 2 (Eef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007907.2

ORF Insert Sequence:

ATGGTGAACT TCACAGTAGA TCAGATCCGT GCCATCATGG ACAAGAAAGC CAACATCCGG 
AACATGTCAG TCATCGCCCA TGTGGACCAC GGCAAGTCCA CGCTGACCGA CTCCCTTGTG 
TGCAAGGCTG GCATCATTGC CTCTGCCCGA GCTGGGGAGA CGCGCTTCAC TGACACTCGC 
AAGGATGAGC AGGAGCGCTG CATCACAATC AAATCCACCG CCATCTCCCT CTTCTACGAG 
CTCTCTGAGA ACGACCTGAA CTTCATTAAG CAGAGCAAGG ATGGCTCGGG CTTCCTCATC 
AACCTCATCG ACTCTCCAGG CCATGTGGAC TTCTCTTCAG AGGTGACAGC TGCCTTGCGT 
GTCACCGATG GAGCTCTGGT GGTGGTGGAC TGTGTGTCTG GCGTGTGTGT GCAGACAGAA 
ACTGTGCTGC GCCAGGCCAT CGCTGAACGC ATCAAGCCCG TCCTGATGAT GAACAAGATG 
GACCGGGCCC TGCTGGAGCT GCAGCTGGAG CCCGAGGAGC TCTACCAGAC CTTCCAGCGC 
ATCGTGGAGA ACGTCAACGT CATCATCTCT ACCTACGGCG AGGGCGAGAG TGGGCCCATG 
GGCAATATCA TGATTGACCC CGTCCTGGGC ACCGTAGGCT TTGGTTCTGG CCTGCATGGC 
TGGGCCTTCA CCCTGAAGCA GTTTGCGGAG ATGTATGTGG CCAAGTTTGC AGCCAAGGGT 
GAGGGCCAGC TGAGCGCAGC CGAGCGTGCC AAGAAAGTAG AGGACATGAT GAAGAAGCTG 
TGGGGAGACC GGTACTTTGA TCCGGCCAAT GGCAAGTTCA GTAAGTCAGC CAATAGCCCA 
GATGGGAAAA AACTTCCCCG CACCTTCTGC CAGCTCATCC TGGACCCCAT CTTCAAGGTG 
TTCGACGCCA TCATGAACTT CAGGAAGGAG GAGACAGCCA AGCTGATCGA GAAGCTGGAC 
ATCAAGCTGG ACAGCGAGGA CAAAGACAAG GAGGGCAAGC CATTGCTCAA GGCTGTGATG 
CGCCGCTGGC TGCCTGCAGG GGACGCCCTC CTGCAGATGA TCACCATCCA CTTACCATCC 
CCCGTCACTG CACAGAAGTA CCGTTGTGAG CTGCTATACG AGGGGCCTCC CGACGATGAG 
GCCGCCATGG GTATTAAGAG CTGCGACCCC AAAGGCCCAC TTATGATGTA CATTTCCAAG 
ATGGTGCCAA CCTCTGACAA AGGCCGCTTC TATGCCTTTG GTAGAGTGTT CTCTGGGGTG 
GTGTCCACGG GCCTGAAGGT CCGGATCATG GGCCCCAACT ACACGCCTGG GAAGAAAGAG 
GACCTATACC TGAAGCCTAT CCAGAGAACC ATTCTGATGA TGGGCCGCTA CGTGGAGCCG 
ATTGAGGACG TGCCATGTGG AAACATTGTG GGGCTGGTCG GAGTGGACCA GTTCCTTGTG 
AAGACGGGGA CCATCACTAC CTTTGAGCAC GCTCACAACA TGCGCGTGAT GAAGTTCAGC 
GTCAGCCCTG TCGTCCGTGT GGCAGTGGAG GCCAAGAACC CAGCTGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGGCT GGCTAAGTCT GACCCTATGG TGCAGTGCAT CATTGAGGAG 
TCTGGAGAAC ATATTATTGC TGGCGCCGGT GAGCTGCACC TGGAGATCTG CCTTAAGGAC 
CTGGAGGAGG ACCATGCCTG CATCCCCATC AAGAAATCTG ACCCTGTTGT GTCATATCGG 
GAGACAGTCA GTGAGGAATC CAACGTGCTG TGTCTGTCCA AGTCCCCCAA TAAGCACAAC 
CGGCTGTACA TGAAGGCCAG GCCCTTCCCT GATGGCCTGG CAGAGGACAT CGATAAGGGT 
GAGGTGTCTG CCCGCCAGGA GCTCAAGGCA CGTGCCCGCT ACCTGGCCGA AAAGTATGAG 
TGGGACGTTG CTGAAGCCCG CAAGATCTGG TGCTTTGGCC CTGATGGCAC TGGCCCCAAC 
ATTCTCACCG ACATCACCAA GGGTGTGCAG TACCTGAATG AGATCAAGGA CAGTGTGGTG 
GCTGGCTTCC AGTGGGCTAC TAAGGAGGGC GCTCTCTGTG AGGAAAACAT GCGTGGTGTG 
CGGTTTGATG TTCATGATGT GACCCTGCAT GCTGATGCCA TTCACCGGGG AGGTGGCCAG 
ATCATCCCCA CAGCACGCCG CTGCCTGTAT GCCAGTGTGC TGACCGCACA GCCCCGCCTC 
ATGGAGCCTA TCTATCTGGT GGAGATCCAG TGTCCTGAGC AAGTGGTGGG TGGCATCTAC 
GGTGTCCTGA ACAGGAAGCG TGGCCATGTG TTTGAGGAGT CCCAGGTGGC TGGTACCCCC 
ATGTTTGTGG TCAAGGCATA CCTGCCTGTC AATGAGTCCT TTGGCTTCAC CGCTGATCTG 
CGATCCAACA CCGGCGGCCA GGCCTTCCCC CAGTGCGTGT TTGACCACTG GCAGATCCTG 
CCTGGGGATC CTTTTGACAA CAGCAGCCGC CCCAGCCAAG TGGTAGCTGA GACGCGCAAG 
CGCAAGGGCC TGAAAGAGGG CATCCCAGCG CTGGACAACT TCCTGGACAA ACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease.
Cell reports22(2)482-496(2018 Jan)
Fakruddin M,Wei FY,Suzuki T,Asano K,Kaieda T,Omori A,Izumi R,Fujimura A,Kaitsuka T,Miyata K,Araki K,Oike Y,Scorrano L,Suzuki T,Tomizawa K

Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis.
Biochemical and biophysical research communications494(1-2)278-284(2017 12)
Deng Z,Luo P,Lai W,Song T,Peng J,Wei HK

Solid tumor therapy by selectively targeting stromal endothelial cells.
Proceedings of the National Academy of Sciences of the United States of America113(28)E4079-87(2016 07)
Liu S,Liu J,Ma Q,Cao L,Fattah RJ,Yu Z,Bugge TH,Finkel T,Leppla SH

Temporally defined neocortical translation and polysome assembly are determined by the RNA-binding protein Hu antigen R.
Proceedings of the National Academy of Sciences of the United States of America111(36)E3815-24(2014 Sep)
Kraushar ML,Thompson K,Wijeratne HR,Viljetic B,Sakers K,Marson JW,Kontoyiannis DL,Buyske S,Hart RP,Rasin MR

IL-6 impairs myogenic differentiation by downmodulation of p90RSK/eEF2 and mTOR/p70S6K axes, without affecting AKT activity.
BioMed research international2014206026(2014)
Pelosi M,De Rossi M,Barberi L,Musar? A

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Eef2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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