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CCL2 cDNA ORF clone, Bos taurus (cattle)

Gene Symbol CCL2
Entrez Gene ID 281043
Full Name chemokine (C-C motif) ligand 2
Synonyms MCP-1, MCP-1A, MCP1, MCP1A, SCYA2
General protein information
Gene Type protein-coding
Organism Bos taurus (cattle)
Genome

19

Summary lac of sum
Disorder MIM:

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_174006 NP_776431 C-C motif chemokine 2 precursor

KEGG
bta04060 Cytokine-cytokine receptor interaction
bta04621 NOD-like receptor signaling pathway
bta04062 Chemokine signaling pathway
bta05142 Chagas disease (American trypanosomiasis)
bta05144 Malaria
bta05323 Rheumatoid arthritis
bta05164 Influenza A
bta05168 Herpes simplex infection
bta04668 TNF signaling pathway


Homo sapiens (human) CCL2 NP_002973.1
Pan troglodytes (chimpanzee) CCL2 XP_001174551.1
Macaca mulatta (Rhesus monkey) CCL2 NP_001027993.1
Canis lupus familiaris (dog) CCL2 NP_001003297.1
Bos taurus (cattle) CCL2 NP_776431.1
Mus musculus (house mouse) Ccl12 NP_035461.2
Rattus norvegicus (Norway rat) Ccl12 NP_001099292.1

Component

ID Name Evidence
GO:0005576 extracellular region IEA
GO:0005615 extracellular space IEA

Function

ID Name Evidence
GO:0008009 chemokine activity IEA

Process

ID Name Evidence
GO:0002548 monocyte chemotaxis IEA
GO:0006916 anti-apoptosis IEA
GO:0006935 chemotaxis IEA
GO:0006955 immune response IEA
GO:0009617 response to bacterium IEA
GO:0019221 cytokine-mediated signaling pathway IEA
GO:0048246 macrophage chemotaxis IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CCL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OBa41647 NM_174006 Bos taurus chemokine (C-C motif) ligand 2 (CCL2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $149.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OBa41647
Accession Version NM_174006.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-JAN-2015
Organism Bos taurus (cattle)
Product C-C motif chemokine 2 precursor
Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M84602.1. On Jun 3, 2003 this sequence version replaced gi:27806710. ##Evidence-Data-START## Transcript exon combination :: M84602.1, BC142093.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145420 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
ATGAAGGTCT CCGCTGCCCT CCTGTGCCTG CTACTCACAG TAGCTGCCTT CAGCACCGAG 
GTGCTCGCTC AGCCAGATGC AATTAACTCC CAAGTCGCCT GCTGCTATAC ATTCAACAGT
AAGAAGATCT CCATGCAGAG GCTGATGAAC TACAGAAGAG TCACCAGCAG CAAGTGTCCT
AAAGAGGCTG TGATTTTCAA GACCATCCTG GGCAAGGAGT TATGTGCAGA CCCCAAGCAG
AAATGGGTCC AGGACTCCAT AAACTATCTT AACAAGAAAA ACCAAACTCC AAAGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_776431.1
CDS45..344
Misc Feature(1)114..116(+)
Misc Feature(2)144..317(+)
Misc Feature(3)144..317(+)
Misc Feature(4)150..317(+)
Misc Feature(5)150..269(+)
Misc Feature(6)150..266(+)
Misc Feature(7)150..185(+)
Misc Feature(8)156..317(+)
Misc Feature(9)162..260(+)
Misc Feature(10)201..308(+)
Misc Feature(11)216..221(+)
Misc Feature(12)246..254(+)
Translation
Position Chain Variation Link
249 249 c, t dbSNP:41255714
523 523 a, g dbSNP:134596426
595 595 c, t dbSNP:136795933
634 634 a, t dbSNP:379319935

Target ORF information:

RefSeq Version NM_174006
Organism Bos taurus (cattle)
Definition Bos taurus chemokine (C-C motif) ligand 2 (CCL2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_174006

ORF Insert Sequence:

1
61
121
181
241
ATGAAGGTCT CCGCTGCCCT CCTGTGCCTG CTACTCACAG TAGCTGCCTT CAGCACCGAG 
GTGCTCGCTC AGCCAGATGC AATTAACTCC CAAGTCGCCT GCTGCTATAC ATTCAACAGT
AAGAAGATCT CCATGCAGAG GCTGATGAAC TACAGAAGAG TCACCAGCAG CAAGTGTCCT
AAAGAGGCTG TGATTTTCAA GACCATCCTG GGCAAGGAGT TATGTGCAGA CCCCAAGCAG
AAATGGGTCC AGGACTCCAT AAACTATCTT AACAAGAAAA ACCAAACTCC AAAGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Allelic gene expression imbalance of bovine IGF2, LEP and CCL2 genes in liver, kidney and pituitary
Mol. Biol. Rep. 40 (2), 1189-1200 (2013)
Olbromski R, Siadkowska E, Zelazowska B and Zwierzchowski L.


book

A whole-genome assembly of the domestic cow, Bos taurus
Genome Biol. 10 (4), R42 (2009)
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marcais G, Roberts M, Subramanian P, Yorke JA and Salzberg SL.


book

Cooperative expression of monocyte chemoattractant protein 1 within the bovine corpus luteum: evidence of immune cell-endothelial cell interactions in a coculture system
Biol. Reprod. 72 (5), 1169-1176 (2005)
Liptak AR, Sullivan BT, Henkes LE, Wijayagunawardane MP, Miyamoto A, Davis JS, Rueda BR and Townson DH.


book

Characterization of the bovine monocyte chemoattractant protein-1 gene
Biochem. Biophys. Res. Commun. 202 (3), 1272-1279 (1994)
Wempe F, Kuhlmann JK and Scheit KH.


book

Characterization by cDNA cloning of the mRNA of a new growth factor from bovine seminal plasma: acidic seminal fluid protein
Biochem. Biophys. Res. Commun. 183 (1), 232-237 (1992)
Wempe F, Einspanier R and Scheit KH.


book

Gene expression and cDNA cloning identified a major basic protein constituent of bovine seminal plasma as bovine monocyte-chemoattractant protein-1 (MCP-1)
DNA Cell Biol. 10 (9), 671-679 (1991)
Wempe F, Henschen A and Scheit KH.


 
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