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COL4A4 collagen, type IV, alpha 4 [Bos taurus (cattle)]

Gene Symbol COL4A4
Entrez Gene ID 407107
Full Name collagen, type IV, alpha 4
Synonyms
General protein information
Names
collagen, type IV, alpha 4
alpha type IV collagen basement membrane
Gene Type protein-coding
Organism Bos taurus (cattle)
Genome

2

Summary lac of sum
Disorder MIM:

Disorder Html:





The following COL4A4 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A4 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
XM_002703716 PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X4, partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD Quote Price
OBa14837 XM_005200219 PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OBa45690 XM_010826602 PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $369
OBa45691 XM_010826603 PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $369
OBa02994 XM_005197528 PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $369
OBa45692 XM_010802516 PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID
Accession Version XM_002703716.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 30-DEC-2014
Organism Bos taurus (cattle)
Product collagen alpha-4(IV) chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001494692.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced gi:528932337. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_002703762.3
CDS<1..1283
Misc Feature(1)252..467(+)
Misc Feature(2)603..926(+)
Misc Feature(3)927..1274(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_002703716
Organism Bos taurus (cattle)
Definition PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X4, partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_002703716

ORF Insert Sequence:

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa14837
Accession Version XM_005200219.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 30-DEC-2014
Organism Bos taurus (cattle)
Product collagen alpha-4(IV) chain isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001503559.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced gi:528933779. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGTGGTTTT CATTCTTGAT TTCTCTTGGA GGCATGACGG GACCTCCTGG GCCACGAGGA 
TTTCCTGGTC ACCCAGGATT TCCAGGGGCA GCTGGGGTTC CTGGAAGAGC TGATTCTGCT
CCAGGCAAAC CAGGCAACCA GGGACCAACT GGGCTGCCTG GAGTGCCGGG ACTGCCGGGA
CCTCCGGGAT CAGATGCTGT ATATTGTACT GTTGGGCAAC CCGGACCACA AGGAACAAAA
GGCAAAGTGG GTCCTCCAGG AAGACGAGGC TCCAAAGGAG AAAAAGGGAG CTCAGGGCTC
TGTGCCTGCC AGCCTGGTCC CATGGGCCCA TCAGGCCCTC CAGGGCCTCC TGGGAGGCAA
GGGAGTAAGG GAGACTTGGG GCTCCCTGGG TGGCTTGGAG AAAAAGGTTC CCCAGGCCCT
CCTGGTGCCA AAGGATCTCC AGGGCCACCG GGAAAACCTG GTGCCTCAGG ACTACCTGGC
AGCAAAGGAG AAAAAGGTGA CATGGTTGTA TCAAGAGTGA AAGGACACAA AGGAGAAAGA
GGGCCTGATG GGCCCCCTGG ACTTCCAGGG CAGCAGGGAC AACACGGTCG AGATGGATAT
GCTGGAGAAA AGGGGGACAC AGGGCCGCCT GGTGATCACG AGGATGCAGC CCCAGGTGAT
AAAGGGCCTC CTGGACCGCC AGGGCCCCCT GGCAGAGCGG GACCTAGGGG GCCCCCAGGC
CTGGGATTTC CTGGCCCTCC AGGAGACAGA GGGCAACCAG GAGCTCCAGG CTACCCAGGC
AACAGGGGTC CTGATGGTCG GAAGGGTCGG AAAGGTGATA CAATTCCTTG TAACGTAACA
TACCCTGGGA GGCCAGGCCC TCCAGGTTTT GATGGTCCTC CAGGTCCAAA GGGACTTCCA
GGTCATCGGG GTGCTCCTGG GCTGAGGTGT TTGGATGGAC AGAAGGGTCA ACGTGGCAAA
CCAGGAATCT CAGAAAGACC TGGTCCACCT GGTTTTCGTG GTGATATAGG AGAACCAGGT
TTGGGAGGTG AAAAGGGGTC CTCGCCTGTT GGGCCCCCAG GCACTCCAGG TTCACCTGGA
GTGAATGGTC AGAAAGGACC CCCAGGAGAT CCTGCTATCG GCTACCCAGG ACTCCCGGGA
AAGAGCGGTC TTCCAGGAGC ACCAGGGTCC AAAGGACTCA GAGGCGATGC CGGGCGTCCA
GGGACCGCAG GGCCAGCTGG CATGCCCGGA TTTCCGGGTC TCCAAGGTCC CAAAGGCCGA
GAGGGAAATG CTGGGTTTCC AGGGATCCCA GGCCCACCTG GTCACTCCTG TGAAAGAGGT
GCTCCAGGGA TACCAGGGCA ACCGGGGCTC CCTGGGGCTC CAGGCAGTCC AGGTGCCCCA
GGTTGGAAAG GACAGCGAGG GGACATGGGA CCTCTGGGAC CTGCTGGAAT GAAGGGCCTT
CCTGGAGTCC CAGGATGGCC GGGGGCAGAT GGAACCCCAG GTCTTCCAGG GGTCCCAGGC
CCCACTGGAG ACGATGGGCT ACCTGGTCTT CCAGGCCCAA AGGGACCCCA GGGGCTGCCT
GGCTTCCTGG GTTTTCCAGG AGAGAGAGGA AAACCTGGTC AGGATGGACA CCCTGGCAGA
AAGGGAGAAC ATGGAGAGAA GGGTTGGCCT GGCTTCCTGG GAGACCGAGG ATTGAAAGGT
GTCAAAGGAG CAAGAGGACC CCCGGGATAT GAAGGAGAAA CGGATATTAT TTCCAAGAAG
GGGGAAACTG GAGAACCTGG ACCTTCTGGG GATGGTGGAT TCCCAGGAGA AAATGGTGAT
AAAGGTCATC CTGGGATGCA AGGAAGGAGA GGAGAGCCAG GAAGCCATGG ACCACCTGGA
TTTCACCCTG GGGAGCCCGG AAGAAATGGA CAGCCAGGGC TTCCTGGACC CCCAGGCCCT
CCAGGCTCAC CTGGGCTGAG AGGAAACATT GGTTTTCCAG GATTTCCGGG TGACCAGGGT
GAGCCAGGTT CTCCAGGGTG CCCGGGACCT TCAGGAATGG ATGGTACAAG AGGACCTAAA
GGAGACAAAG GGGAATCTGC AAGTCAGTTT GGCCTGCCTG GTCCAAAGGG TGAGCCGGGT
GGCCCTGGAT ATCCAGGACG TTTGGGAGCA TCTGGAGAGC AGGGCTTGCC CGGTGCTCAA
GGTCCTGGAG GACCACCCGG AAGGCCAGGA CCTCCTGGCT TGTCTGGACC AACAGGATGT
CCAGGTAGTC CAGGGGGGCC TGGGCTGCAG GGACCCCCAG GAGAAGTGGG GGATCCTGGA
CCAAGAGGCG TCCTGGGGGA CCCAGGAGCA CCAGGTCTTC CAGGAATAAA AGGTAAGTCA
GAAGGAACCC TGACCCCTCA GAAAGTTCTA GCCAATGATG CTCCCACAAG TTCAGAGTGC
CTCTCTTGCA CCCCAGGGAC ATGCTCTGGG GTCCCCCTTT TTTCCGGGAT GCCTGTGGAC
ACCTCCTTTT CTTTCTTACT CAGGCAGCAC TGGCCTGGTC TCCTGACCGG CTCTACCAAA
ATGATGCTCA TTTTTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005200276.1
CDS413..2950
Misc Feature(1)1586..1795(+)
Misc Feature(2)1838..2014(+)
Misc Feature(3)1928..2107(+)
Misc Feature(4)2099..2272(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_005200219
Organism Bos taurus (cattle)
Definition PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005200219

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGTGGTTTT CATTCTTGAT TTCTCTTGGA GGCATGACGG GACCTCCTGG GCCACGAGGA 
TTTCCTGGTC ACCCAGGATT TCCAGGGGCA GCTGGGGTTC CTGGAAGAGC TGATTCTGCT
CCAGGCAAAC CAGGCAACCA GGGACCAACT GGGCTGCCTG GAGTGCCGGG ACTGCCGGGA
CCTCCGGGAT CAGATGCTGT ATATTGTACT GTTGGGCAAC CCGGACCACA AGGAACAAAA
GGCAAAGTGG GTCCTCCAGG AAGACGAGGC TCCAAAGGAG AAAAAGGGAG CTCAGGGCTC
TGTGCCTGCC AGCCTGGTCC CATGGGCCCA TCAGGCCCTC CAGGGCCTCC TGGGAGGCAA
GGGAGTAAGG GAGACTTGGG GCTCCCTGGG TGGCTTGGAG AAAAAGGTTC CCCAGGCCCT
CCTGGTGCCA AAGGATCTCC AGGGCCACCG GGAAAACCTG GTGCCTCAGG ACTACCTGGC
AGCAAAGGAG AAAAAGGTGA CATGGTTGTA TCAAGAGTGA AAGGACACAA AGGAGAAAGA
GGGCCTGATG GGCCCCCTGG ACTTCCAGGG CAGCAGGGAC AACACGGTCG AGATGGATAT
GCTGGAGAAA AGGGGGACAC AGGGCCGCCT GGTGATCACG AGGATGCAGC CCCAGGTGAT
AAAGGGCCTC CTGGACCGCC AGGGCCCCCT GGCAGAGCGG GACCTAGGGG GCCCCCAGGC
CTGGGATTTC CTGGCCCTCC AGGAGACAGA GGGCAACCAG GAGCTCCAGG CTACCCAGGC
AACAGGGGTC CTGATGGTCG GAAGGGTCGG AAAGGTGATA CAATTCCTTG TAACGTAACA
TACCCTGGGA GGCCAGGCCC TCCAGGTTTT GATGGTCCTC CAGGTCCAAA GGGACTTCCA
GGTCATCGGG GTGCTCCTGG GCTGAGGTGT TTGGATGGAC AGAAGGGTCA ACGTGGCAAA
CCAGGAATCT CAGAAAGACC TGGTCCACCT GGTTTTCGTG GTGATATAGG AGAACCAGGT
TTGGGAGGTG AAAAGGGGTC CTCGCCTGTT GGGCCCCCAG GCACTCCAGG TTCACCTGGA
GTGAATGGTC AGAAAGGACC CCCAGGAGAT CCTGCTATCG GCTACCCAGG ACTCCCGGGA
AAGAGCGGTC TTCCAGGAGC ACCAGGGTCC AAAGGACTCA GAGGCGATGC CGGGCGTCCA
GGGACCGCAG GGCCAGCTGG CATGCCCGGA TTTCCGGGTC TCCAAGGTCC CAAAGGCCGA
GAGGGAAATG CTGGGTTTCC AGGGATCCCA GGCCCACCTG GTCACTCCTG TGAAAGAGGT
GCTCCAGGGA TACCAGGGCA ACCGGGGCTC CCTGGGGCTC CAGGCAGTCC AGGTGCCCCA
GGTTGGAAAG GACAGCGAGG GGACATGGGA CCTCTGGGAC CTGCTGGAAT GAAGGGCCTT
CCTGGAGTCC CAGGATGGCC GGGGGCAGAT GGAACCCCAG GTCTTCCAGG GGTCCCAGGC
CCCACTGGAG ACGATGGGCT ACCTGGTCTT CCAGGCCCAA AGGGACCCCA GGGGCTGCCT
GGCTTCCTGG GTTTTCCAGG AGAGAGAGGA AAACCTGGTC AGGATGGACA CCCTGGCAGA
AAGGGAGAAC ATGGAGAGAA GGGTTGGCCT GGCTTCCTGG GAGACCGAGG ATTGAAAGGT
GTCAAAGGAG CAAGAGGACC CCCGGGATAT GAAGGAGAAA CGGATATTAT TTCCAAGAAG
GGGGAAACTG GAGAACCTGG ACCTTCTGGG GATGGTGGAT TCCCAGGAGA AAATGGTGAT
AAAGGTCATC CTGGGATGCA AGGAAGGAGA GGAGAGCCAG GAAGCCATGG ACCACCTGGA
TTTCACCCTG GGGAGCCCGG AAGAAATGGA CAGCCAGGGC TTCCTGGACC CCCAGGCCCT
CCAGGCTCAC CTGGGCTGAG AGGAAACATT GGTTTTCCAG GATTTCCGGG TGACCAGGGT
GAGCCAGGTT CTCCAGGGTG CCCGGGACCT TCAGGAATGG ATGGTACAAG AGGACCTAAA
GGAGACAAAG GGGAATCTGC AAGTCAGTTT GGCCTGCCTG GTCCAAAGGG TGAGCCGGGT
GGCCCTGGAT ATCCAGGACG TTTGGGAGCA TCTGGAGAGC AGGGCTTGCC CGGTGCTCAA
GGTCCTGGAG GACCACCCGG AAGGCCAGGA CCTCCTGGCT TGTCTGGACC AACAGGATGT
CCAGGTAGTC CAGGGGGGCC TGGGCTGCAG GGACCCCCAG GAGAAGTGGG GGATCCTGGA
CCAAGAGGCG TCCTGGGGGA CCCAGGAGCA CCAGGTCTTC CAGGAATAAA AGGTAAGTCA
GAAGGAACCC TGACCCCTCA GAAAGTTCTA GCCAATGATG CTCCCACAAG TTCAGAGTGC
CTCTCTTGCA CCCCAGGGAC ATGCTCTGGG GTCCCCCTTT TTTCCGGGAT GCCTGTGGAC
ACCTCCTTTT CTTTCTTACT CAGGCAGCAC TGGCCTGGTC TCCTGACCGG CTCTACCAAA
ATGATGCTCA TTTTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa45690
Accession Version XM_010826602.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1296bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 30-DEC-2014
Organism Bos taurus (cattle)
Product collagen alpha-4(IV) chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001494688.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG GGATCTTCCC
AACCCAGGGA TTGAACCTGC GGCTCCTACC GCGTCTCCTG CATTGCACGC AAACTCTTTT
ACTTCTGTGC CACTGGCAAA GCCCAACTCA TCTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_010824904.1
CDS242..1537
Misc Feature(1)1130..1291(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_010826602
Organism Bos taurus (cattle)
Definition PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_010826602

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG GGATCTTCCC
AACCCAGGGA TTGAACCTGC GGCTCCTACC GCGTCTCCTG CATTGCACGC AAACTCTTTT
ACTTCTGTGC CACTGGCAAA GCCCAACTCA TCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa45691
Accession Version XM_010826603.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1260bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 30-DEC-2014
Organism Bos taurus (cattle)
Product collagen alpha-4(IV) chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001494688.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG TATGAATCCC
CTGGCTTTTA CAGCTCAGGT TTGCTACATC CTCCTCCACA CCAGGGGTTC TCCATTCTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_010824905.1
CDS242..1501
Misc Feature(1)1130..1291(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_010826603
Organism Bos taurus (cattle)
Definition PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_010826603

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG TATGAATCCC
CTGGCTTTTA CAGCTCAGGT TTGCTACATC CTCCTCCACA CCAGGGGTTC TCCATTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa02994
Accession Version XM_005197528.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1212bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 30-DEC-2014
Organism Bos taurus (cattle)
Product collagen alpha-4(IV) chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001494688.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced gi:528900990. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG TTCAACCTTT
ACAAGTTGTT GA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005197585.1
CDS242..1453
Misc Feature(1)1130..1291(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_005197528
Organism Bos taurus (cattle)
Definition PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005197528

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG TTCAACCTTT
ACAAGTTGTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa45692
Accession Version XM_010802516.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4995bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 30-DEC-2014
Organism Bos taurus (cattle)
Product collagen alpha-4(IV) chain isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
ATGTCATGTG CAGATCTAGG GAAAGAGCCT TCGAGGAAGT GCTTGCACCG AGGCCCTGGG 
ACAGATAAGA GCAGCGTGCT GGATGCAGTG AGTGTGGGGC GGGCACAGTA CAGGGCGCAA
GGGAATCTGA GGCCACACTC TGTGAGGCCT GGAAGACCCA CTGGGATCCC AGGGGAGAAA
GGGATGAAAG GTGACAGTGG CCGTCCTGGA GCAGCAGGTG ACGTAGGTGA CAAGGGTCCA
ACCGGAGTTC CTGGATTTCC AGGTCTGGAT GGGATACCTG GGCACCCAGG GCCTCCTGGA
TCCAGAGGCA GACCTGGCAT AGATGGCTAC AATGGCTCAA GAGGTGATCC AGGGATTCCA
GGAGAAAGAG GAGCTCTTGG CCCACGAGGG CCCCCAGGCC TTCCTGGAGA AAAAGGAGAA
AAAGGAAATT CAGTGTTCAT TTTAGGTGCC ACTAAAGGTA TGCAGGGCGA CAGAGGGGAC
CCAGGACCTC CCGGCTCACC AGGATCAAGG GGAGTGAGAG GCCCAGCAGG CCCAGTGGGG
TACCCAGGAG AGCCCGGGTT AGCAGGACCT CCAGGCCCCC CTGGGAGTCC AGGCTTAAAG
GGTAATCCTG GCGTGGGAGT AAAGGGGCAA ATGGGAGACC CGGGTGAGGT TGGCCAACAA
GGTTCTCCGG GGCCCACTCT GTTGGTGCAG CCACCTGATT CTGGTCTCTA TAAAGGAGAA
AAGGGTATTA AAGGAATGCC TGGAATGACT GGACTTCCTG GACCACCGGG ACCCAGGGGA
GAGCCTGGTA TTGGGAGAAA AGGAGAGAAA GGGATTCCTG GGTTTCCAGG ACCTCGGGGG
GACCCTGGTT CCTATGGATC TCCAGGTTTT CCAGGATTAA AGGGGGAACC AGGACTGTTT
GGAGATCCTG GGTCATTTGG ATTTCTTGGC CCAAAGGGAG ATCCTGGAGA CCGAGGGCAC
CCAGGACCAC CAGGGGTTTT GGTAACTCCA TCTCTTCCAC TCAAAGGCCC TCCAGGGGAT
CCAGGGCGCC CTGGCCGCTA TGGAGAAACA GGGGCTGTTG GACCACGTGG TCCTCCTGGT
GCCCCAGGTA GACCAGGGGA AGCCTGTCCA GGCATGACGG GACCTCCTGG GCCACGAGGA
TTTCCTGGTC ACCCAGGATT TCCAGGGGCA GCTGGGGTTC CTGGAAGAGC TGATTCTGCT
CCAGGCAAAC CAGGCAACCA GGGACCAACT GGGCTGCCTG GAGTGCCGGG ACTGCCGGGA
CCTCCGGGAT CAGATGCTGT ATATTGTACT GTTGGGCAAC CCGGACCACA AGGAACAAAA
GGCAAAGTGG GTCCTCCAGG AAGACGAGGC TCCAAAGGAG AAAAAGGGAG CTCAGGGCTC
TGTGCCTGCC AGCCTGGTCC CATGGGCCCA TCAGGCCCTC CAGGGCCTCC TGGGAGGCAA
GGGAGTAAGG GAGACTTGGG GCTCCCTGGG TGGCTTGGAG AAAAAGGTTC CCCAGGCCCT
CCTGGTGCCA AAGGATCTCC AGGGCCACCG GGAAAACCTG GTGCCTCAGG ACTACCTGGC
AGCAAAGGAG AAAAAGGTGA CATGGTTGTA TCAAGAGTGA AAGGACACAA AGGAGAAAGA
GGGCCTGATG GGCCCCCTGG ACTTCCAGGG CAGCAGGGAC AACACGGTCG AGATGGATAT
GCTGGAGAAA AGGGGGACAC AGGGCCGCCT GGTGATCACG AGGATGCAGC CCCAGGTGAT
AAAGGGCCTC CTGGACCGCC AGGGCCCCCT GGCAGAGCGG GACCTAGGGG GCCCCCAGGC
CTGGGATTTC CTGGCCCTCC AGGAGACAGA GGGCAACCAG GAGCTCCAGG CTACCCAGGC
AACAGGGGTC CTGATGGTCG GAAGGGTCGG AAAGGTGATA CAATTCCTTG TAACGTAACA
TACCCTGGGA GGCCAGGCCC TCCAGGTTTT GATGGTCCTC CAGGTCCAAA GGGACTTCCA
GGTCATCGGG GTGCTCCTGG GCTGAGGTGT TTGGATGGAC AGAAGGGTCA ACGTGGCAAA
CCAGGAATCT CAGAAAGACC TGGTCCACCT GGTTTTCGTG GTGATATAGG AGAACCAGGT
TTGGGAGGTG AAAAGGGGTC CTCGCCTGTT GGGCCCCCAG GCACTCCAGG TTCACCTGGA
GTGAATGGTC AGAAAGGACC CCCAGGAGAT CCTGCTATCG GCTACCCAGG ACTCCCGGGA
AAGAGCGGTC TTCCAGGAGC ACCAGGGTCC AAAGGACTCA GAGGCGATGC CGGGCGTCCA
GGGACCGCAG GGCCAGCTGG CATGCCCGGA TTTCCGGGTC TCCAAGGTCC CAAAGGCCGA
GAGGGAAATG CTGGGTTTCC AGGGATCCCA GGCCCACCTG GTCACTCCTG TGAAAGAGGT
GCTCCAGGGA TACCAGGGCA ACCGGGGCTC CCTGGGGCTC CAGGCAGTCC AGGTGCCCCA
GGTTGGAAAG GACAGCGAGG GGACATGGGA CCTCTGGGAC CTGCTGGAAT GAAGGGCCTT
CCTGGAGTCC CAGGATGGCC GGGGGCAGAT GGAACCCCAG GTCTTCCAGG GGTCCCAGGC
CCCACTGGAG ACGATGGGCT ACCTGGTCTT CCAGGCCCAA AGGGACCCCA GGGGCTGCCT
GGCTTCCTGG GTTTTCCAGG AGAGAGAGGA AAACCTGGTC AGGATGGACA CCCTGGCAGA
AAGGGAGAAC ATGGAGAGAA GGGTTGGCCT GGCTTCCTGG GAGACCGAGG ATTGAAAGGT
GTCAAAGGAG CAAGAGGACC CCCGGGATAT GAAGGAGAAA CGGATATTAT TTCCAAGAAG
GGGGAAACTG GAGAACCTGG ACCTTCTGGG GATGGTGGAT TCCCAGGAGA AAATGGTGAT
AAAGGTCATC CTGGGATGCA AGGAAGGAGA GGAGAGCCAG GAAGCCATGG ACCACCTGGA
TTTCACCCTG GGGAGCCCGG AAGAAATGGA CAGCCAGGGC TTCCTGGACC CCCAGGCCCT
CCAGGCTCAC CTGGGCTGAG AGGAAACATT GGTTTTCCAG GATTTCCGGG TGACCAGGGT
GAGCCAGGTT CTCCAGGGTG CCCGGGACCT TCAGGAATGG ATGGTACAAG AGGACCTAAA
GGAGACAAAG GGGAATCTGC AAGTCAGTTT GGCCTGCCTG GTCCAAAGGG TGAGCCGGGT
GGCCCTGGAT ATCCAGGACG TTTGGGAGCA TCTGGAGAGC AGGGCTTGCC CGGTGCTCAA
GGTCCTGGAG GACCACCCGG AAGGCCAGGA CCTCCTGGCT TGTCTGGACC AACAGGATGT
CCAGGTAGTC CAGGGGGGCC TGGGCTGCAG GGACCCCCAG GAGAAGTGGG GGATCCTGGA
CCAAGAGGCG TCCTGGGGGA CCCAGGAGCA CCAGGTCTTC CAGGAATAAA AGGTAAGTCA
GAAGGAACCC TGACCCCTCA GAAAGTTCTA GCCAATGATG CTCCCACAAG TTCAGAGTGC
CTCTCTTGCA CCCCAGGGAC ATGCTCTGGG GTCCCCCTTT TTTCCGGGAT GCCTGTGGAC
ACCTCCTTTT CTTTCTTACT CAGGCAGCAC TGGCCTGGTC TCCTGACCGG CTCTACCAAA
ATGATAGCTG GCAAGTGTCT AAGGAAAGCA GCACCACCAC CCTTTGCCGT GACTGTCCCA
TACCTCTTGG ACCGTCGGGG CCAGGAGGCC CCTGATCTCC AGGCAGACCC GGAATCTTTT
TGCCTACTCG TGTGGTCAGG AGTACCCGGG CCTCCAGGAC CACCTGGGAG TGTCGACCTT
CTGAAAGGAG AACCAGGAGA CTGTGGTCTG CCAGGGCCTC CAGGTCTCCC GGGCCCACCA
GGCCCTCCAG GACACAAGGG CTTCCCAGGA TGTGATGGAA AACATGGCCA GAAAGGACCA
ATGGGATTCC CGGGGCCGCA GGGGCCACCT GGAAGTCCTG GGCCACCTGG AGACAAAGGT
TTACCTGGAC CTCCAGGCAG ACGAGGGCCT TTGGGTCCTC CGGGTTCCAG AGGTGAGCCT
GGGCCCCCAG CAGACTTGGA TGCCTGCCCC CGAATCCCAG GGCTTCCTGG GGTACCAGGC
CCCAGAGGAC CGGAAGGAAC CATGGGGCTT CCTGGCATGA GAGGCCCGCC GGGACCAGGG
TGCAAAGGAG AGCCTGGGCT GGATGGCAGG AGGGGTGAGG ATGGCCTCCC AGGGTCCCCT
GGGCCTCCGG GACATAAAGG TGACATGGGA GAAGCAGGCT GTCCAGGAGC ACCAGGCCCT
CCTGGGCCCA TGGGGGATCC TGGGCCCATA GGGTTTGGCC CTGGATACCT CAGTGGCTTC
CTCCTGGTTC TCCACAGTCA GACGGATGGA GAACCCACCT GCCCCATGGG CATGCCCAGG
CTCTGGACTG GGTATAGTCT GCTATACCTG GAAGGACAGG AGAGAGCTCA CAATCAAGAC
CTCGGTCTGG CGGGCTCCTG CCTCCCCATC TTTAGCACGC TGCCCTTCGC CTACTGCAAC
ATCCACCAGG TGTGCCACTA TGCCCGGAGG AACGACAGGT CCTACTGGCT GGCGAGCACA
GCGCCTCTGC CCATGACTCC GCTCTCGGAG GACGAGATCC GCCCGTACAT CAGCCGCTGC
GCAGTGTGCG AGGCCCCCGC CCAGGCCGTG GCGGTGCACA GCCAAGACCA GTCCATCCCC
CCATGTCCGC GGGCCTGGAG GAGCCTCTGG ATCGGGTACT CCTTCCTCAT GCACACAGGC
GCTGGGGACC AAGGAGGCGG GCAGGCCCTC ATGTCCCCAG GCAGCTGCCT AGAGGATTTC
AGAGCCGCAC CGTTCCTGGA ATGCCAAGGC CGGCAGGGAA CTTGCCACTT TTTTGCCAAC
AAGTATAGTT TCTGGCTGAC AACAGTGAGA CCTGACTTGC AGTTTTCCTC TGCACCATTA
CCGGACACCT TGAAAGAAAG CCATGCTCAA CGCCAGAAAA TCAGCAGGTG CCAGGTCTGC
GTGAAGCATA GCTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_010800818.1
CDS1..4995
Misc Feature(1)796..972(+)
Misc Feature(2)2254..2463(+)
Misc Feature(3)2506..2682(+)
Misc Feature(4)2596..2775(+)
Misc Feature(5)4315..4638(+)
Misc Feature(6)4639..4986(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_010802516
Organism Bos taurus (cattle)
Definition PREDICTED: Bos taurus collagen, type IV, alpha 4 (COL4A4), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_010802516

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
ATGTCATGTG CAGATCTAGG GAAAGAGCCT TCGAGGAAGT GCTTGCACCG AGGCCCTGGG 
ACAGATAAGA GCAGCGTGCT GGATGCAGTG AGTGTGGGGC GGGCACAGTA CAGGGCGCAA
GGGAATCTGA GGCCACACTC TGTGAGGCCT GGAAGACCCA CTGGGATCCC AGGGGAGAAA
GGGATGAAAG GTGACAGTGG CCGTCCTGGA GCAGCAGGTG ACGTAGGTGA CAAGGGTCCA
ACCGGAGTTC CTGGATTTCC AGGTCTGGAT GGGATACCTG GGCACCCAGG GCCTCCTGGA
TCCAGAGGCA GACCTGGCAT AGATGGCTAC AATGGCTCAA GAGGTGATCC AGGGATTCCA
GGAGAAAGAG GAGCTCTTGG CCCACGAGGG CCCCCAGGCC TTCCTGGAGA AAAAGGAGAA
AAAGGAAATT CAGTGTTCAT TTTAGGTGCC ACTAAAGGTA TGCAGGGCGA CAGAGGGGAC
CCAGGACCTC CCGGCTCACC AGGATCAAGG GGAGTGAGAG GCCCAGCAGG CCCAGTGGGG
TACCCAGGAG AGCCCGGGTT AGCAGGACCT CCAGGCCCCC CTGGGAGTCC AGGCTTAAAG
GGTAATCCTG GCGTGGGAGT AAAGGGGCAA ATGGGAGACC CGGGTGAGGT TGGCCAACAA
GGTTCTCCGG GGCCCACTCT GTTGGTGCAG CCACCTGATT CTGGTCTCTA TAAAGGAGAA
AAGGGTATTA AAGGAATGCC TGGAATGACT GGACTTCCTG GACCACCGGG ACCCAGGGGA
GAGCCTGGTA TTGGGAGAAA AGGAGAGAAA GGGATTCCTG GGTTTCCAGG ACCTCGGGGG
GACCCTGGTT CCTATGGATC TCCAGGTTTT CCAGGATTAA AGGGGGAACC AGGACTGTTT
GGAGATCCTG GGTCATTTGG ATTTCTTGGC CCAAAGGGAG ATCCTGGAGA CCGAGGGCAC
CCAGGACCAC CAGGGGTTTT GGTAACTCCA TCTCTTCCAC TCAAAGGCCC TCCAGGGGAT
CCAGGGCGCC CTGGCCGCTA TGGAGAAACA GGGGCTGTTG GACCACGTGG TCCTCCTGGT
GCCCCAGGTA GACCAGGGGA AGCCTGTCCA GGCATGACGG GACCTCCTGG GCCACGAGGA
TTTCCTGGTC ACCCAGGATT TCCAGGGGCA GCTGGGGTTC CTGGAAGAGC TGATTCTGCT
CCAGGCAAAC CAGGCAACCA GGGACCAACT GGGCTGCCTG GAGTGCCGGG ACTGCCGGGA
CCTCCGGGAT CAGATGCTGT ATATTGTACT GTTGGGCAAC CCGGACCACA AGGAACAAAA
GGCAAAGTGG GTCCTCCAGG AAGACGAGGC TCCAAAGGAG AAAAAGGGAG CTCAGGGCTC
TGTGCCTGCC AGCCTGGTCC CATGGGCCCA TCAGGCCCTC CAGGGCCTCC TGGGAGGCAA
GGGAGTAAGG GAGACTTGGG GCTCCCTGGG TGGCTTGGAG AAAAAGGTTC CCCAGGCCCT
CCTGGTGCCA AAGGATCTCC AGGGCCACCG GGAAAACCTG GTGCCTCAGG ACTACCTGGC
AGCAAAGGAG AAAAAGGTGA CATGGTTGTA TCAAGAGTGA AAGGACACAA AGGAGAAAGA
GGGCCTGATG GGCCCCCTGG ACTTCCAGGG CAGCAGGGAC AACACGGTCG AGATGGATAT
GCTGGAGAAA AGGGGGACAC AGGGCCGCCT GGTGATCACG AGGATGCAGC CCCAGGTGAT
AAAGGGCCTC CTGGACCGCC AGGGCCCCCT GGCAGAGCGG GACCTAGGGG GCCCCCAGGC
CTGGGATTTC CTGGCCCTCC AGGAGACAGA GGGCAACCAG GAGCTCCAGG CTACCCAGGC
AACAGGGGTC CTGATGGTCG GAAGGGTCGG AAAGGTGATA CAATTCCTTG TAACGTAACA
TACCCTGGGA GGCCAGGCCC TCCAGGTTTT GATGGTCCTC CAGGTCCAAA GGGACTTCCA
GGTCATCGGG GTGCTCCTGG GCTGAGGTGT TTGGATGGAC AGAAGGGTCA ACGTGGCAAA
CCAGGAATCT CAGAAAGACC TGGTCCACCT GGTTTTCGTG GTGATATAGG AGAACCAGGT
TTGGGAGGTG AAAAGGGGTC CTCGCCTGTT GGGCCCCCAG GCACTCCAGG TTCACCTGGA
GTGAATGGTC AGAAAGGACC CCCAGGAGAT CCTGCTATCG GCTACCCAGG ACTCCCGGGA
AAGAGCGGTC TTCCAGGAGC ACCAGGGTCC AAAGGACTCA GAGGCGATGC CGGGCGTCCA
GGGACCGCAG GGCCAGCTGG CATGCCCGGA TTTCCGGGTC TCCAAGGTCC CAAAGGCCGA
GAGGGAAATG CTGGGTTTCC AGGGATCCCA GGCCCACCTG GTCACTCCTG TGAAAGAGGT
GCTCCAGGGA TACCAGGGCA ACCGGGGCTC CCTGGGGCTC CAGGCAGTCC AGGTGCCCCA
GGTTGGAAAG GACAGCGAGG GGACATGGGA CCTCTGGGAC CTGCTGGAAT GAAGGGCCTT
CCTGGAGTCC CAGGATGGCC GGGGGCAGAT GGAACCCCAG GTCTTCCAGG GGTCCCAGGC
CCCACTGGAG ACGATGGGCT ACCTGGTCTT CCAGGCCCAA AGGGACCCCA GGGGCTGCCT
GGCTTCCTGG GTTTTCCAGG AGAGAGAGGA AAACCTGGTC AGGATGGACA CCCTGGCAGA
AAGGGAGAAC ATGGAGAGAA GGGTTGGCCT GGCTTCCTGG GAGACCGAGG ATTGAAAGGT
GTCAAAGGAG CAAGAGGACC CCCGGGATAT GAAGGAGAAA CGGATATTAT TTCCAAGAAG
GGGGAAACTG GAGAACCTGG ACCTTCTGGG GATGGTGGAT TCCCAGGAGA AAATGGTGAT
AAAGGTCATC CTGGGATGCA AGGAAGGAGA GGAGAGCCAG GAAGCCATGG ACCACCTGGA
TTTCACCCTG GGGAGCCCGG AAGAAATGGA CAGCCAGGGC TTCCTGGACC CCCAGGCCCT
CCAGGCTCAC CTGGGCTGAG AGGAAACATT GGTTTTCCAG GATTTCCGGG TGACCAGGGT
GAGCCAGGTT CTCCAGGGTG CCCGGGACCT TCAGGAATGG ATGGTACAAG AGGACCTAAA
GGAGACAAAG GGGAATCTGC AAGTCAGTTT GGCCTGCCTG GTCCAAAGGG TGAGCCGGGT
GGCCCTGGAT ATCCAGGACG TTTGGGAGCA TCTGGAGAGC AGGGCTTGCC CGGTGCTCAA
GGTCCTGGAG GACCACCCGG AAGGCCAGGA CCTCCTGGCT TGTCTGGACC AACAGGATGT
CCAGGTAGTC CAGGGGGGCC TGGGCTGCAG GGACCCCCAG GAGAAGTGGG GGATCCTGGA
CCAAGAGGCG TCCTGGGGGA CCCAGGAGCA CCAGGTCTTC CAGGAATAAA AGGTAAGTCA
GAAGGAACCC TGACCCCTCA GAAAGTTCTA GCCAATGATG CTCCCACAAG TTCAGAGTGC
CTCTCTTGCA CCCCAGGGAC ATGCTCTGGG GTCCCCCTTT TTTCCGGGAT GCCTGTGGAC
ACCTCCTTTT CTTTCTTACT CAGGCAGCAC TGGCCTGGTC TCCTGACCGG CTCTACCAAA
ATGATAGCTG GCAAGTGTCT AAGGAAAGCA GCACCACCAC CCTTTGCCGT GACTGTCCCA
TACCTCTTGG ACCGTCGGGG CCAGGAGGCC CCTGATCTCC AGGCAGACCC GGAATCTTTT
TGCCTACTCG TGTGGTCAGG AGTACCCGGG CCTCCAGGAC CACCTGGGAG TGTCGACCTT
CTGAAAGGAG AACCAGGAGA CTGTGGTCTG CCAGGGCCTC CAGGTCTCCC GGGCCCACCA
GGCCCTCCAG GACACAAGGG CTTCCCAGGA TGTGATGGAA AACATGGCCA GAAAGGACCA
ATGGGATTCC CGGGGCCGCA GGGGCCACCT GGAAGTCCTG GGCCACCTGG AGACAAAGGT
TTACCTGGAC CTCCAGGCAG ACGAGGGCCT TTGGGTCCTC CGGGTTCCAG AGGTGAGCCT
GGGCCCCCAG CAGACTTGGA TGCCTGCCCC CGAATCCCAG GGCTTCCTGG GGTACCAGGC
CCCAGAGGAC CGGAAGGAAC CATGGGGCTT CCTGGCATGA GAGGCCCGCC GGGACCAGGG
TGCAAAGGAG AGCCTGGGCT GGATGGCAGG AGGGGTGAGG ATGGCCTCCC AGGGTCCCCT
GGGCCTCCGG GACATAAAGG TGACATGGGA GAAGCAGGCT GTCCAGGAGC ACCAGGCCCT
CCTGGGCCCA TGGGGGATCC TGGGCCCATA GGGTTTGGCC CTGGATACCT CAGTGGCTTC
CTCCTGGTTC TCCACAGTCA GACGGATGGA GAACCCACCT GCCCCATGGG CATGCCCAGG
CTCTGGACTG GGTATAGTCT GCTATACCTG GAAGGACAGG AGAGAGCTCA CAATCAAGAC
CTCGGTCTGG CGGGCTCCTG CCTCCCCATC TTTAGCACGC TGCCCTTCGC CTACTGCAAC
ATCCACCAGG TGTGCCACTA TGCCCGGAGG AACGACAGGT CCTACTGGCT GGCGAGCACA
GCGCCTCTGC CCATGACTCC GCTCTCGGAG GACGAGATCC GCCCGTACAT CAGCCGCTGC
GCAGTGTGCG AGGCCCCCGC CCAGGCCGTG GCGGTGCACA GCCAAGACCA GTCCATCCCC
CCATGTCCGC GGGCCTGGAG GAGCCTCTGG ATCGGGTACT CCTTCCTCAT GCACACAGGC
GCTGGGGACC AAGGAGGCGG GCAGGCCCTC ATGTCCCCAG GCAGCTGCCT AGAGGATTTC
AGAGCCGCAC CGTTCCTGGA ATGCCAAGGC CGGCAGGGAA CTTGCCACTT TTTTGCCAAC
AAGTATAGTT TCTGGCTGAC AACAGTGAGA CCTGACTTGC AGTTTTCCTC TGCACCATTA
CCGGACACCT TGAAAGAAAG CCATGCTCAA CGCCAGAAAA TCAGCAGGTG CCAGGTCTGC
GTGAAGCATA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.