×

COL2A1 cDNA ORF clone, Bos taurus (cattle)

Gene Symbol COL2A1
Entrez Gene ID 407142
Full Name collagen, type II, alpha 1
Synonyms CB11
General protein information
Preferred Names
collagen alpha-1(II) chain
Names
collagen alpha-1(II) chain
pro-alpha 1(II)
cyanogen bromide
alpha-1 type II collagen
Gene Type protein-coding
Organism Bos taurus (cattle)
Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_001001135 NP_001001135 collagen alpha-1(II) chain isoform 1 precursor
NM_001113224 NP_001106695 collagen alpha-1(II) chain isoform 2 precursor

KEGG
bta04512 ECM-receptor interaction
bta04510 Focal adhesion
bta05146 Amoebiasis
bta04974 Protein digestion and absorption
bta04151 PI3K-Akt signaling pathway
bta04611 Platelet activation
WikiPathways
WP1065 Endochondral Ossification


Homo sapiens (human) COL2A1 NP_001835.3
Pan troglodytes (chimpanzee) COL2A1 XP_509026.2
Macaca mulatta (Rhesus monkey) COL2A1 XP_001100739.1
Canis lupus familiaris (dog) COL2A1 NP_001006952.1
Bos taurus (cattle) COL2A1 NP_001001135.2
Mus musculus (house mouse) Col2a1 NP_112440.2
Rattus norvegicus (Norway rat) Col2a1 NP_037061.1
Gallus gallus (chicken) COL2A1 NP_989757.1
Danio rerio (zebrafish) col2a1a NP_571367.1
Xenopus (Silurana) tropicalis (western clawed frog) col2a1 NP_989220.1

Component

ID Name Evidence
GO:0005576 extracellular region IEA
GO:0005581 collagen IEA
GO:0005585 collagen type II IEA
GO:0005604 basement membrane IEA
GO:0005615 extracellular space IEA
GO:0005737 cytoplasm IEA
GO:0031012 extracellular matrix IEA

Function

ID Name Evidence
GO:0005201 extracellular matrix structural constituent IEA
GO:0048407 platelet-derived growth factor binding IEA

Process

ID Name Evidence
GO:0001502 cartilage condensation IEA
GO:0001894 tissue homeostasis IEA
GO:0001958 endochondral ossification IEA
GO:0002062 chondrocyte differentiation IEA
GO:0003007 heart morphogenesis IEA
GO:0006029 proteoglycan metabolic process IEA
GO:0007601 visual perception IEA
GO:0007605 sensory perception of sound IEA
GO:0010468 regulation of gene expression IEA
GO:0030199 collagen fibril organization IEA
GO:0035108 limb morphogenesis IEA
GO:0042472 inner ear morphogenesis IEA
GO:0043066 negative regulation of apoptosis IEA
GO:0060021 palate development IEA
GO:0060272 embryonic skeletal joint morphogenesis IEA
GO:0060348 bone development IEA
GO:0060351 cartilage development involved in endochondral bone morphogenesis IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following COL2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OBa01441 NM_001001135 Bos taurus collagen, type II, alpha 1 (COL2A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00
OBa01444 NM_001113224 Bos taurus collagen, type II, alpha 1 (COL2A1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $797.30
$1139.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OBa01441
Clone ID Related Accession (Same CDS sequence) NM_001001135
Accession Version NM_001001135.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-JAN-2015
Organism Bos taurus (cattle)
Product collagen alpha-1(II) chain isoform 1 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03017085.1, AAFC03017082.1 and AAFC03056593.1. On Jan 1, 2008 this sequence version replaced gi:47564047. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment of the human homolog (NM_001844.4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093753, SAMN02404548 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCAGAAG GCTGGCAGCT GTGTGCAGGA CGGGCAGAGG
TATAATGATA AGGATGTGTG GAAGCCCGAG CCCTGCCGGA TCTGTGTCTG TGACACTGGG
ACTGTCCTCT GCGACGACAT AATCTGTGAA GACATGAAAG ACTGCCTCAG CCCCGAGACC
CCCTTCGGAG AGTGCTGCCC CATCTGCTCA GCTGACCTCC CCACTGCCAG TGGGCAACCA
GGACCAAAGG GACAGAAAGG AGAACCTGGA GATATCAAGG ACATCGTAGG ACCCAAGGGA
CCTCCTGGAC CTCAGGGACC CGCAGGTGAA CAAGGACCCA GAGGTGATCG TGGTGACAAA
GGTGAAAAAG GTGCTCCTGG ACCTCGTGGC AGAGATGGAG AGCCTGGGAC CCCTGGAAAC
CCTGGCCCCC CTGGTCCTCC CGGCCCCCCT GGCCCCCCTG GCCTTGGTGG AAACTTTGCT
GCCCAGATGG CTGGTGGATT TGATGAGAAG GCTGGTGGCG CCCAGATGGG AGTAATGCAG
GGACCAATGG GCCCCATGGG ACCTCGGGGA CCCCCAGGCC CTGCTGGTGC TCCCGGACCT
CAAGGATTTC AAGGCAACCC TGGTGAACCT GGTGAACCCG GTGTTTCTGG TCCCATGGGC
CCCCGTGGTC CTCCTGGCCC CCCTGGGAAA CCTGGTGATG ATGGTGAAGC TGGAAAGCCT
GGAAAATCTG GCGAGAGAGG CCCTCCTGGC CCTCAGGGTG CTCGTGGTTT CCCAGGAACC
CCAGGCCTTC CTGGTGTCAA AGGTCACAGA GGTTACCCAG GTCTAGATGG TGCTAAGGGA
GAAGCTGGTG CTCCAGGTGT GAAGGGTGAG AGTGGTTCCC CAGGTGAGAA CGGTTCTCCA
GGCCCAATGG GTCCCCGCGG CTTGCCTGGT GAGAGAGGAC GGACTGGCCC TGCCGGCGCT
GCAGGTGCTC GAGGCAATGA CGGTCAGCCA GGCCCTGCAG GGCCTCCGGG TCCTGTGGGT
CCTGCTGGCG GTCCTGGCTT TCCTGGTGCT CCTGGTGCCA AGGGTGAAGC TGGCCCCACC
GGTGCTCGAG GTCCCGAAGG CGCCCAGGGT CCTCGCGGTG AACCGGGTAC TCCTGGGTCC
CCTGGGCCAG CTGGTGCTGC TGGCAACCCT GGAACTGATG GAATCCCTGG AGCCAAGGGA
TCTGCTGGTG CCCCTGGCAT TGCTGGTGCT CCCGGCTTCC CTGGACCCCG TGGTCCACCC
GGCCCTCAAG GTGCAACTGG TCCTCTGGGC CCAAAAGGTC AAACGGGTGA GCCTGGTATT
GCTGGCTTCA AAGGCGAACA AGGCCCCAAG GGAGAACCGG GCCCTGCTGG TCCCCAAGGA
GCCCCTGGTC CTGCTGGTGA AGAAGGGAAA AGAGGTGCCC GTGGAGAACC TGGTGGTGCT
GGGCCCGCCG GTCCCCCTGG AGAAAGAGGC GCTCCTGGAA ACCGTGGTTT CCCAGGTCAG
GATGGTCTGG CAGGTCCCAA GGGAGCCCCT GGAGAGCGAG GACCCAGTGG CCTCGCTGGT
CCCAAAGGCG CCAACGGTGA CCCTGGCCGT CCCGGAGAGC CTGGCCTTCC TGGAGCCCGG
GGTCTCACTG GTCGCCCTGG TGATGCTGGT CCTCAAGGCA AAGTTGGTCC TTCCGGAGCC
CCTGGTGAAG ACGGTCGCCC TGGACCCCCA GGTCCTCAGG GGGCTCGTGG GCAGCCTGGT
GTCATGGGTT TCCCTGGCCC CAAAGGTGCC AATGGTGAGC CTGGCAAAGC TGGTGAGAAA
GGACTGCCTG GTGCTCCTGG TCTGAGAGGT CTTCCTGGCA AAGATGGTGA GACAGGTGCT
GCAGGCCCCC CTGGACCCGC TGGACCAGCT GGTGAACGAG GCGAGCAGGG TGCTCCTGGG
CCATCTGGGT TCCAGGGACT TCCTGGCCCT CCCGGCCCCC CAGGTGAAGG TGGAAAACCA
GGTGACCAGG GTGTTCCTGG TGAAGCTGGA GCTCCCGGCC TCGTGGGTCC CAGGGGTGAA
CGAGGTTTCC CAGGAGAACG TGGCTCCCCC GGTTCCCAGG GCCTCCAGGG GGCCCGCGGT
CTCCCCGGCA CTCCTGGCAC TGATGGTCCC AAAGGCGCAG CTGGCCCAGC TGGCCCCCCT
GGGGCTCAGG GCCCTCCAGG TCTGCAGGGC ATGCCCGGTG AGAGGGGAGC AGCTGGTATC
GCCGGACCCA AGGGAGACAG GGGTGATGTT GGTGAGAAAG GCCCCGAGGG CGCCCCCGGG
AAGGACGGTG GACGAGGTCT GACTGGCCCC ATTGGCCCCC CTGGCCCAGC CGGAGCCAAC
GGTGAGAAGG GAGAAGTTGG ACCCCCTGGT CCTGCTGGAA CTGCTGGTGC TCGAGGTGCC
CCGGGTGAAC GTGGAGAGAC AGGCCCCCCT GGACCTGCTG GATTCGCCGG TCCCCCTGGT
GCTGATGGCC AGCCTGGTGC CAAAGGTGAA CAAGGAGAAG CCGGCCAGAA AGGTGACGCT
GGTGCCCCCG GTCCTCAGGG CCCCTCTGGA GCTCCTGGGC CTCAGGGTCC TACTGGTGTG
ACCGGTCCTA AAGGAGCCCG AGGTGCTCAA GGGCCCCCGG GAGCCACCGG ATTCCCCGGA
GCAGCTGGCC GCGTCGGACC CCCAGGCTCC AATGGCAACC CTGGACCCCC TGGTCCTCCT
GGTCCTTCTG GAAAAGATGG TCCTAAAGGT GCTCGAGGCG ACAGTGGGCC CCCTGGCCGA
GCTGGTGACC CTGGCCTCCA AGGTCCTGCT GGACCCCCTG GCGAGAAGGG AGAGCCTGGA
GATGATGGTC CTTCTGGTCC CGATGGTCCT CCAGGTCCTC AGGGTCTGGC TGGTCAGAGG
GGCATCGTTG GTCTGCCTGG ACAGCGTGGT GAGCGAGGAT TCCCCGGCCT CCCCGGCCCC
TCGGGTGAGC CTGGCAAGCA GGGAGCCCCT GGAGCATCTG GAGACCGAGG TCCCCCTGGC
CCTGTGGGTC CTCCTGGCCT GACTGGTCCT GCTGGTGAGC CTGGACGTGA GGGAAGCCCC
GGTGCTGACG GCCCCCCTGG CAGAGATGGC GCAGCTGGAG TCAAAGGTGA CCGTGGAGAG
ACCGGTGCCG TGGGTGCTCC TGGAGCCCCT GGGCCCCCTG GCTCTCCTGG CCCCGCTGGC
CCAATTGGCA AGCAGGGAGA CCGAGGAGAA GCTGGCGCAC AAGGTCCCAT GGGACCTGCA
GGACCTGCTG GAGCCCGGGG AATGCCAGGC CCTCAAGGCC CCCGAGGTGA CAAAGGAGAA
ACTGGAGAGG CTGGCGAGAG GGGACTGAAG GGACACCGTG GCTTCACTGG TCTGCAGGGT
CTGCCCGGCC CCCCTGGTCC TTCTGGAGAC CAGGGTGCTT CCGGTCCTGC AGGTCCTTCC
GGCCCCAGAG GTCCTCCTGG TCCCGTCGGT CCCTCTGGCA AAGATGGTGC TAACGGAATC
CCTGGCCCCA TTGGACCTCC TGGGCCCCGT GGACGTTCCG GCGAGACTGG CCCTGCTGGT
CCTCCTGGAA ACCCTGGACC CCCTGGCCCT CCTGGCCCCC CCGGCCCTGG CATCGACATG
TCCGCCTTTG CTGGCCTTGG CCAGAGAGAG AAAGGCCCCG ATCCTCTGCA GTACATGCGG
GCTGACGAGG CAGCCGGCAA CCTGAGACAG CATGATGCCG AGGTGGACGC CACACTCAAG
TCCCTCAACA ACCAGATCGA GAGCCTCCGT AGCCCCGAGG GCTCCCGCAA GAACCCAGCT
CGCACCTGCC GAGACCTGAA ACTCTGCCAC CCTGAGTGGA AGAGCGGAGA CTACTGGATC
GACCCCAACC AGGGCTGCAC CCTGGATGCC ATGAAGGTTT TCTGCAACAT GGAGACTGGC
GAGACCTGCG TCTACCCCAA CCCGGCCAGC GTCCCCAAGA AGAACTGGTG GAGCAGCAAG
AGCAAGGACA AGAAACACAT CTGGTTTGGA GAAACCATCA ACGGTGGCTT CCACTTCAGC
TATGGAGATG ACAACCTGGC TCCCAACACC GCCAACGTCC AGATGACCTT CCTGCGCCTG
CTGTCCACCG AGGGCTCTCA GAACATCACC TACCACTGCA AGAACAGCAT TGCCTACCTG
GACGAAGCTG CTGGCAACCT CAAGAAGGCT CTGCTCATCC AGGGCTCCAA CGACGTGGAG
ATCCGGGCTG AGGGCAACAG CAGGTTCACA TATACCGTTC TGAAGGATGG CTGCACGAAA
CACACCGGTA AGTGGGGCAA GACTATGATC GAGTACCGGT CACAGAAGAC CTCCCGTCTG
CCCATCATTG ACATTGCACC CATGGACATA GGAGGGCCCG AGCAGGAATT CGGTGTGGAC
ATAGGGCCTG TCTGCTTCTT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001001135.2
CDS27..4490
Misc Feature(1)21..23(+)
Misc Feature(2)126..293(+)
Misc Feature(3)567..572(+)
Misc Feature(4)627..3668(+)
Misc Feature(5)660..662(+)
Misc Feature(6)678..680(+)
Misc Feature(7)714..716(+)
Misc Feature(8)723..725(+)
Misc Feature(9)759..761(+)
Misc Feature(10)768..770(+)
Misc Feature(11)777..779(+)
Misc Feature(12)798..977(+)
Misc Feature(13)804..806(+)
Misc Feature(14)831..833(+)
Misc Feature(15)858..860(+)
Misc Feature(16)867..869(+)
Misc Feature(17)876..878(+)
Misc Feature(18)885..887(+)
Misc Feature(19)903..905(+)
Misc Feature(20)921..923(+)
Misc Feature(21)939..941(+)
Misc Feature(22)948..950(+)
Misc Feature(23)966..968(+)
Misc Feature(24)984..986(+)
Misc Feature(25)1011..1013(+)
Misc Feature(26)1074..1076(+)
Misc Feature(27)1092..1094(+)
Misc Feature(28)1119..1121(+)
Misc Feature(29)1128..1130(+)
Misc Feature(30)1137..1139(+)
Misc Feature(31)1146..1148(+)
Misc Feature(32)1149..1301(+)
Misc Feature(33)1209..1211(+)
Misc Feature(34)1218..1220(+)
Misc Feature(35)1227..1229(+)
Misc Feature(36)1254..1256(+)
Misc Feature(37)1272..1274(+)
Misc Feature(38)1281..1283(+)
Misc Feature(39)1299..1301(+)
Misc Feature(40)1317..1319(+)
Misc Feature(41)1326..1328(+)
Misc Feature(42)1344..1346(+)
Misc Feature(43)1365..1505(+)
Misc Feature(44)1380..1382(+)
Misc Feature(45)1398..1400(+)
Misc Feature(46)1416..1418(+)
Misc Feature(47)1434..1436(+)
Misc Feature(48)1443..1445(+)
Misc Feature(49)1470..1472(+)
Misc Feature(50)1515..1517(+)
Misc Feature(51)1542..1544(+)
Misc Feature(52)1551..1706(+)
Misc Feature(53)1560..1562(+)
Misc Feature(54)1578..1580(+)
Misc Feature(55)1605..1607(+)
Misc Feature(56)1614..1616(+)
Misc Feature(57)1650..1652(+)
Misc Feature(58)1662..1877(+)
Misc Feature(59)1677..1679(+)
Misc Feature(60)1695..1697(+)
Misc Feature(61)1722..1724(+)
Misc Feature(62)1767..1769(+)
Misc Feature(63)1785..1787(+)
Misc Feature(64)1794..1796(+)
Misc Feature(65)1821..1823(+)
Misc Feature(66)1839..1841(+)
Misc Feature(67)1848..1850(+)
Misc Feature(68)1866..1868(+)
Misc Feature(69)1884..1886(+)
Misc Feature(70)1893..1895(+)
Misc Feature(71)1902..1904(+)
Misc Feature(72)1920..1922(+)
Misc Feature(73)1956..1958(+)
Misc Feature(74)2001..2003(+)
Misc Feature(75)2028..2030(+)
Misc Feature(76)2037..2039(+)
Misc Feature(77)2046..2048(+)
Misc Feature(78)2076..>2204(+)
Misc Feature(79)2427..2606(+)
Misc Feature(80)2535..2711(+)
Misc Feature(81)2832..3044(+)
Misc Feature(82)3279..3410(+)
Misc Feature(83)3669..3749(+)
Misc Feature(84)3747..3752(+)
Misc Feature(85)3780..4487(+)
Translation
Position Chain Variation Link
103 103 a, g dbSNP:134559128
1508 1508 g, t dbSNP:110274310
2156 2156 c, t dbSNP:108981059
2402 2402 c, t dbSNP:209731964
2719 2719 a, c dbSNP:109700727
3236 3236 c, t dbSNP:384560704
3719 3719 a, g dbSNP:382996583
3749 3749 c, t dbSNP:110943643
3836 3836 c, t dbSNP:136487089
4819 4819 g, t dbSNP:210517944
4936 4936 g, t dbSNP:133539847

Target ORF information:

RefSeq Version NM_001001135
Organism Bos taurus (cattle)
Definition Bos taurus collagen, type II, alpha 1 (COL2A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001001135

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCAGAAG GCTGGCAGCT GTGTGCAGGA CGGGCAGAGG
TATAATGATA AGGATGTGTG GAAGCCCGAG CCCTGCCGGA TCTGTGTCTG TGACACTGGG
ACTGTCCTCT GCGACGACAT AATCTGTGAA GACATGAAAG ACTGCCTCAG CCCCGAGACC
CCCTTCGGAG AGTGCTGCCC CATCTGCTCA GCTGACCTCC CCACTGCCAG TGGGCAACCA
GGACCAAAGG GACAGAAAGG AGAACCTGGA GATATCAAGG ACATCGTAGG ACCCAAGGGA
CCTCCTGGAC CTCAGGGACC CGCAGGTGAA CAAGGACCCA GAGGTGATCG TGGTGACAAA
GGTGAAAAAG GTGCTCCTGG ACCTCGTGGC AGAGATGGAG AGCCTGGGAC CCCTGGAAAC
CCTGGCCCCC CTGGTCCTCC CGGCCCCCCT GGCCCCCCTG GCCTTGGTGG AAACTTTGCT
GCCCAGATGG CTGGTGGATT TGATGAGAAG GCTGGTGGCG CCCAGATGGG AGTAATGCAG
GGACCAATGG GCCCCATGGG ACCTCGGGGA CCCCCAGGCC CTGCTGGTGC TCCCGGACCT
CAAGGATTTC AAGGCAACCC TGGTGAACCT GGTGAACCCG GTGTTTCTGG TCCCATGGGC
CCCCGTGGTC CTCCTGGCCC CCCTGGGAAA CCTGGTGATG ATGGTGAAGC TGGAAAGCCT
GGAAAATCTG GCGAGAGAGG CCCTCCTGGC CCTCAGGGTG CTCGTGGTTT CCCAGGAACC
CCAGGCCTTC CTGGTGTCAA AGGTCACAGA GGTTACCCAG GTCTAGATGG TGCTAAGGGA
GAAGCTGGTG CTCCAGGTGT GAAGGGTGAG AGTGGTTCCC CAGGTGAGAA CGGTTCTCCA
GGCCCAATGG GTCCCCGCGG CTTGCCTGGT GAGAGAGGAC GGACTGGCCC TGCCGGCGCT
GCAGGTGCTC GAGGCAATGA CGGTCAGCCA GGCCCTGCAG GGCCTCCGGG TCCTGTGGGT
CCTGCTGGCG GTCCTGGCTT TCCTGGTGCT CCTGGTGCCA AGGGTGAAGC TGGCCCCACC
GGTGCTCGAG GTCCCGAAGG CGCCCAGGGT CCTCGCGGTG AACCGGGTAC TCCTGGGTCC
CCTGGGCCAG CTGGTGCTGC TGGCAACCCT GGAACTGATG GAATCCCTGG AGCCAAGGGA
TCTGCTGGTG CCCCTGGCAT TGCTGGTGCT CCCGGCTTCC CTGGACCCCG TGGTCCACCC
GGCCCTCAAG GTGCAACTGG TCCTCTGGGC CCAAAAGGTC AAACGGGTGA GCCTGGTATT
GCTGGCTTCA AAGGCGAACA AGGCCCCAAG GGAGAACCGG GCCCTGCTGG TCCCCAAGGA
GCCCCTGGTC CTGCTGGTGA AGAAGGGAAA AGAGGTGCCC GTGGAGAACC TGGTGGTGCT
GGGCCCGCCG GTCCCCCTGG AGAAAGAGGC GCTCCTGGAA ACCGTGGTTT CCCAGGTCAG
GATGGTCTGG CAGGTCCCAA GGGAGCCCCT GGAGAGCGAG GACCCAGTGG CCTCGCTGGT
CCCAAAGGCG CCAACGGTGA CCCTGGCCGT CCCGGAGAGC CTGGCCTTCC TGGAGCCCGG
GGTCTCACTG GTCGCCCTGG TGATGCTGGT CCTCAAGGCA AAGTTGGTCC TTCCGGAGCC
CCTGGTGAAG ACGGTCGCCC TGGACCCCCA GGTCCTCAGG GGGCTCGTGG GCAGCCTGGT
GTCATGGGTT TCCCTGGCCC CAAAGGTGCC AATGGTGAGC CTGGCAAAGC TGGTGAGAAA
GGACTGCCTG GTGCTCCTGG TCTGAGAGGT CTTCCTGGCA AAGATGGTGA GACAGGTGCT
GCAGGCCCCC CTGGACCCGC TGGACCAGCT GGTGAACGAG GCGAGCAGGG TGCTCCTGGG
CCATCTGGGT TCCAGGGACT TCCTGGCCCT CCCGGCCCCC CAGGTGAAGG TGGAAAACCA
GGTGACCAGG GTGTTCCTGG TGAAGCTGGA GCTCCCGGCC TCGTGGGTCC CAGGGGTGAA
CGAGGTTTCC CAGGAGAACG TGGCTCCCCC GGTTCCCAGG GCCTCCAGGG GGCCCGCGGT
CTCCCCGGCA CTCCTGGCAC TGATGGTCCC AAAGGCGCAG CTGGCCCAGC TGGCCCCCCT
GGGGCTCAGG GCCCTCCAGG TCTGCAGGGC ATGCCCGGTG AGAGGGGAGC AGCTGGTATC
GCCGGACCCA AGGGAGACAG GGGTGATGTT GGTGAGAAAG GCCCCGAGGG CGCCCCCGGG
AAGGACGGTG GACGAGGTCT GACTGGCCCC ATTGGCCCCC CTGGCCCAGC CGGAGCCAAC
GGTGAGAAGG GAGAAGTTGG ACCCCCTGGT CCTGCTGGAA CTGCTGGTGC TCGAGGTGCC
CCGGGTGAAC GTGGAGAGAC AGGCCCCCCT GGACCTGCTG GATTCGCCGG TCCCCCTGGT
GCTGATGGCC AGCCTGGTGC CAAAGGTGAA CAAGGAGAAG CCGGCCAGAA AGGTGACGCT
GGTGCCCCCG GTCCTCAGGG CCCCTCTGGA GCTCCTGGGC CTCAGGGTCC TACTGGTGTG
ACCGGTCCTA AAGGAGCCCG AGGTGCTCAA GGGCCCCCGG GAGCCACCGG ATTCCCCGGA
GCAGCTGGCC GCGTCGGACC CCCAGGCTCC AATGGCAACC CTGGACCCCC TGGTCCTCCT
GGTCCTTCTG GAAAAGATGG TCCTAAAGGT GCTCGAGGCG ACAGTGGGCC CCCTGGCCGA
GCTGGTGACC CTGGCCTCCA AGGTCCTGCT GGACCCCCTG GCGAGAAGGG AGAGCCTGGA
GATGATGGTC CTTCTGGTCC CGATGGTCCT CCAGGTCCTC AGGGTCTGGC TGGTCAGAGG
GGCATCGTTG GTCTGCCTGG ACAGCGTGGT GAGCGAGGAT TCCCCGGCCT CCCCGGCCCC
TCGGGTGAGC CTGGCAAGCA GGGAGCCCCT GGAGCATCTG GAGACCGAGG TCCCCCTGGC
CCTGTGGGTC CTCCTGGCCT GACTGGTCCT GCTGGTGAGC CTGGACGTGA GGGAAGCCCC
GGTGCTGACG GCCCCCCTGG CAGAGATGGC GCAGCTGGAG TCAAAGGTGA CCGTGGAGAG
ACCGGTGCCG TGGGTGCTCC TGGAGCCCCT GGGCCCCCTG GCTCTCCTGG CCCCGCTGGC
CCAATTGGCA AGCAGGGAGA CCGAGGAGAA GCTGGCGCAC AAGGTCCCAT GGGACCTGCA
GGACCTGCTG GAGCCCGGGG AATGCCAGGC CCTCAAGGCC CCCGAGGTGA CAAAGGAGAA
ACTGGAGAGG CTGGCGAGAG GGGACTGAAG GGACACCGTG GCTTCACTGG TCTGCAGGGT
CTGCCCGGCC CCCCTGGTCC TTCTGGAGAC CAGGGTGCTT CCGGTCCTGC AGGTCCTTCC
GGCCCCAGAG GTCCTCCTGG TCCCGTCGGT CCCTCTGGCA AAGATGGTGC TAACGGAATC
CCTGGCCCCA TTGGACCTCC TGGGCCCCGT GGACGTTCCG GCGAGACTGG CCCTGCTGGT
CCTCCTGGAA ACCCTGGACC CCCTGGCCCT CCTGGCCCCC CCGGCCCTGG CATCGACATG
TCCGCCTTTG CTGGCCTTGG CCAGAGAGAG AAAGGCCCCG ATCCTCTGCA GTACATGCGG
GCTGACGAGG CAGCCGGCAA CCTGAGACAG CATGATGCCG AGGTGGACGC CACACTCAAG
TCCCTCAACA ACCAGATCGA GAGCCTCCGT AGCCCCGAGG GCTCCCGCAA GAACCCAGCT
CGCACCTGCC GAGACCTGAA ACTCTGCCAC CCTGAGTGGA AGAGCGGAGA CTACTGGATC
GACCCCAACC AGGGCTGCAC CCTGGATGCC ATGAAGGTTT TCTGCAACAT GGAGACTGGC
GAGACCTGCG TCTACCCCAA CCCGGCCAGC GTCCCCAAGA AGAACTGGTG GAGCAGCAAG
AGCAAGGACA AGAAACACAT CTGGTTTGGA GAAACCATCA ACGGTGGCTT CCACTTCAGC
TATGGAGATG ACAACCTGGC TCCCAACACC GCCAACGTCC AGATGACCTT CCTGCGCCTG
CTGTCCACCG AGGGCTCTCA GAACATCACC TACCACTGCA AGAACAGCAT TGCCTACCTG
GACGAAGCTG CTGGCAACCT CAAGAAGGCT CTGCTCATCC AGGGCTCCAA CGACGTGGAG
ATCCGGGCTG AGGGCAACAG CAGGTTCACA TATACCGTTC TGAAGGATGG CTGCACGAAA
CACACCGGTA AGTGGGGCAA GACTATGATC GAGTACCGGT CACAGAAGAC CTCCCGTCTG
CCCATCATTG ACATTGCACC CATGGACATA GGAGGGCCCG AGCAGGAATT CGGTGTGGAC
ATAGGGCCTG TCTGCTTCTT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa01444
Clone ID Related Accession (Same CDS sequence) NM_001113224
Accession Version NM_001113224.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-JAN-2015
Organism Bos taurus (cattle)
Product collagen alpha-1(II) chain isoform 2 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03017085.1, AAFC03017082.1 and AAFC03056593.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a shorter protein. The resulting protein (isoform 2) lacks the von Willebrand factor type C (VWC) domain, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment of the human homolog (NM_033150.2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093753, SAMN02404548 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCGGCAA CCAGGACCAA AGGGACAGAA AGGAGAACCT
GGAGATATCA AGGACATCGT AGGACCCAAG GGACCTCCTG GACCTCAGGG ACCCGCAGGT
GAACAAGGAC CCAGAGGTGA TCGTGGTGAC AAAGGTGAAA AAGGTGCTCC TGGACCTCGT
GGCAGAGATG GAGAGCCTGG GACCCCTGGA AACCCTGGCC CCCCTGGTCC TCCCGGCCCC
CCTGGCCCCC CTGGCCTTGG TGGAAACTTT GCTGCCCAGA TGGCTGGTGG ATTTGATGAG
AAGGCTGGTG GCGCCCAGAT GGGAGTAATG CAGGGACCAA TGGGCCCCAT GGGACCTCGG
GGACCCCCAG GCCCTGCTGG TGCTCCCGGA CCTCAAGGAT TTCAAGGCAA CCCTGGTGAA
CCTGGTGAAC CCGGTGTTTC TGGTCCCATG GGCCCCCGTG GTCCTCCTGG CCCCCCTGGG
AAACCTGGTG ATGATGGTGA AGCTGGAAAG CCTGGAAAAT CTGGCGAGAG AGGCCCTCCT
GGCCCTCAGG GTGCTCGTGG TTTCCCAGGA ACCCCAGGCC TTCCTGGTGT CAAAGGTCAC
AGAGGTTACC CAGGTCTAGA TGGTGCTAAG GGAGAAGCTG GTGCTCCAGG TGTGAAGGGT
GAGAGTGGTT CCCCAGGTGA GAACGGTTCT CCAGGCCCAA TGGGTCCCCG CGGCTTGCCT
GGTGAGAGAG GACGGACTGG CCCTGCCGGC GCTGCAGGTG CTCGAGGCAA TGACGGTCAG
CCAGGCCCTG CAGGGCCTCC GGGTCCTGTG GGTCCTGCTG GCGGTCCTGG CTTTCCTGGT
GCTCCTGGTG CCAAGGGTGA AGCTGGCCCC ACCGGTGCTC GAGGTCCCGA AGGCGCCCAG
GGTCCTCGCG GTGAACCGGG TACTCCTGGG TCCCCTGGGC CAGCTGGTGC TGCTGGCAAC
CCTGGAACTG ATGGAATCCC TGGAGCCAAG GGATCTGCTG GTGCCCCTGG CATTGCTGGT
GCTCCCGGCT TCCCTGGACC CCGTGGTCCA CCCGGCCCTC AAGGTGCAAC TGGTCCTCTG
GGCCCAAAAG GTCAAACGGG TGAGCCTGGT ATTGCTGGCT TCAAAGGCGA ACAAGGCCCC
AAGGGAGAAC CGGGCCCTGC TGGTCCCCAA GGAGCCCCTG GTCCTGCTGG TGAAGAAGGG
AAAAGAGGTG CCCGTGGAGA ACCTGGTGGT GCTGGGCCCG CCGGTCCCCC TGGAGAAAGA
GGCGCTCCTG GAAACCGTGG TTTCCCAGGT CAGGATGGTC TGGCAGGTCC CAAGGGAGCC
CCTGGAGAGC GAGGACCCAG TGGCCTCGCT GGTCCCAAAG GCGCCAACGG TGACCCTGGC
CGTCCCGGAG AGCCTGGCCT TCCTGGAGCC CGGGGTCTCA CTGGTCGCCC TGGTGATGCT
GGTCCTCAAG GCAAAGTTGG TCCTTCCGGA GCCCCTGGTG AAGACGGTCG CCCTGGACCC
CCAGGTCCTC AGGGGGCTCG TGGGCAGCCT GGTGTCATGG GTTTCCCTGG CCCCAAAGGT
GCCAATGGTG AGCCTGGCAA AGCTGGTGAG AAAGGACTGC CTGGTGCTCC TGGTCTGAGA
GGTCTTCCTG GCAAAGATGG TGAGACAGGT GCTGCAGGCC CCCCTGGACC CGCTGGACCA
GCTGGTGAAC GAGGCGAGCA GGGTGCTCCT GGGCCATCTG GGTTCCAGGG ACTTCCTGGC
CCTCCCGGCC CCCCAGGTGA AGGTGGAAAA CCAGGTGACC AGGGTGTTCC TGGTGAAGCT
GGAGCTCCCG GCCTCGTGGG TCCCAGGGGT GAACGAGGTT TCCCAGGAGA ACGTGGCTCC
CCCGGTTCCC AGGGCCTCCA GGGGGCCCGC GGTCTCCCCG GCACTCCTGG CACTGATGGT
CCCAAAGGCG CAGCTGGCCC AGCTGGCCCC CCTGGGGCTC AGGGCCCTCC AGGTCTGCAG
GGCATGCCCG GTGAGAGGGG AGCAGCTGGT ATCGCCGGAC CCAAGGGAGA CAGGGGTGAT
GTTGGTGAGA AAGGCCCCGA GGGCGCCCCC GGGAAGGACG GTGGACGAGG TCTGACTGGC
CCCATTGGCC CCCCTGGCCC AGCCGGAGCC AACGGTGAGA AGGGAGAAGT TGGACCCCCT
GGTCCTGCTG GAACTGCTGG TGCTCGAGGT GCCCCGGGTG AACGTGGAGA GACAGGCCCC
CCTGGACCTG CTGGATTCGC CGGTCCCCCT GGTGCTGATG GCCAGCCTGG TGCCAAAGGT
GAACAAGGAG AAGCCGGCCA GAAAGGTGAC GCTGGTGCCC CCGGTCCTCA GGGCCCCTCT
GGAGCTCCTG GGCCTCAGGG TCCTACTGGT GTGACCGGTC CTAAAGGAGC CCGAGGTGCT
CAAGGGCCCC CGGGAGCCAC CGGATTCCCC GGAGCAGCTG GCCGCGTCGG ACCCCCAGGC
TCCAATGGCA ACCCTGGACC CCCTGGTCCT CCTGGTCCTT CTGGAAAAGA TGGTCCTAAA
GGTGCTCGAG GCGACAGTGG GCCCCCTGGC CGAGCTGGTG ACCCTGGCCT CCAAGGTCCT
GCTGGACCCC CTGGCGAGAA GGGAGAGCCT GGAGATGATG GTCCTTCTGG TCCCGATGGT
CCTCCAGGTC CTCAGGGTCT GGCTGGTCAG AGGGGCATCG TTGGTCTGCC TGGACAGCGT
GGTGAGCGAG GATTCCCCGG CCTCCCCGGC CCCTCGGGTG AGCCTGGCAA GCAGGGAGCC
CCTGGAGCAT CTGGAGACCG AGGTCCCCCT GGCCCTGTGG GTCCTCCTGG CCTGACTGGT
CCTGCTGGTG AGCCTGGACG TGAGGGAAGC CCCGGTGCTG ACGGCCCCCC TGGCAGAGAT
GGCGCAGCTG GAGTCAAAGG TGACCGTGGA GAGACCGGTG CCGTGGGTGC TCCTGGAGCC
CCTGGGCCCC CTGGCTCTCC TGGCCCCGCT GGCCCAATTG GCAAGCAGGG AGACCGAGGA
GAAGCTGGCG CACAAGGTCC CATGGGACCT GCAGGACCTG CTGGAGCCCG GGGAATGCCA
GGCCCTCAAG GCCCCCGAGG TGACAAAGGA GAAACTGGAG AGGCTGGCGA GAGGGGACTG
AAGGGACACC GTGGCTTCAC TGGTCTGCAG GGTCTGCCCG GCCCCCCTGG TCCTTCTGGA
GACCAGGGTG CTTCCGGTCC TGCAGGTCCT TCCGGCCCCA GAGGTCCTCC TGGTCCCGTC
GGTCCCTCTG GCAAAGATGG TGCTAACGGA ATCCCTGGCC CCATTGGACC TCCTGGGCCC
CGTGGACGTT CCGGCGAGAC TGGCCCTGCT GGTCCTCCTG GAAACCCTGG ACCCCCTGGC
CCTCCTGGCC CCCCCGGCCC TGGCATCGAC ATGTCCGCCT TTGCTGGCCT TGGCCAGAGA
GAGAAAGGCC CCGATCCTCT GCAGTACATG CGGGCTGACG AGGCAGCCGG CAACCTGAGA
CAGCATGATG CCGAGGTGGA CGCCACACTC AAGTCCCTCA ACAACCAGAT CGAGAGCCTC
CGTAGCCCCG AGGGCTCCCG CAAGAACCCA GCTCGCACCT GCCGAGACCT GAAACTCTGC
CACCCTGAGT GGAAGAGCGG AGACTACTGG ATCGACCCCA ACCAGGGCTG CACCCTGGAT
GCCATGAAGG TTTTCTGCAA CATGGAGACT GGCGAGACCT GCGTCTACCC CAACCCGGCC
AGCGTCCCCA AGAAGAACTG GTGGAGCAGC AAGAGCAAGG ACAAGAAACA CATCTGGTTT
GGAGAAACCA TCAACGGTGG CTTCCACTTC AGCTATGGAG ATGACAACCT GGCTCCCAAC
ACCGCCAACG TCCAGATGAC CTTCCTGCGC CTGCTGTCCA CCGAGGGCTC TCAGAACATC
ACCTACCACT GCAAGAACAG CATTGCCTAC CTGGACGAAG CTGCTGGCAA CCTCAAGAAG
GCTCTGCTCA TCCAGGGCTC CAACGACGTG GAGATCCGGG CTGAGGGCAA CAGCAGGTTC
ACATATACCG TTCTGAAGGA TGGCTGCACG AAACACACCG GTAAGTGGGG CAAGACTATG
ATCGAGTACC GGTCACAGAA GACCTCCCGT CTGCCCATCA TTGACATTGC ACCCATGGAC
ATAGGAGGGC CCGAGCAGGA ATTCGGTGTG GACATAGGGC CTGTCTGCTT CTTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001106695.1
CDS182..4438
Misc Feature(1)176..178(+)
Misc Feature(2)746..925(+)
Misc Feature(3)1097..1249(+)
Misc Feature(4)1313..1453(+)
Misc Feature(5)1499..1654(+)
Misc Feature(6)1610..1825(+)
Misc Feature(7)2024..>2152(+)
Misc Feature(8)2375..2554(+)
Misc Feature(9)2483..2659(+)
Misc Feature(10)2780..2992(+)
Misc Feature(11)3227..3358(+)
Misc Feature(12)3728..4435(+)
Translation
Position Chain Variation Link
258 258 a, g dbSNP:134559128
1456 1456 g, t dbSNP:110274310
2104 2104 c, t dbSNP:108981059
2350 2350 c, t dbSNP:209731964
2667 2667 a, c dbSNP:109700727
3184 3184 c, t dbSNP:384560704
3667 3667 a, g dbSNP:382996583
3697 3697 c, t dbSNP:110943643
3784 3784 c, t dbSNP:136487089
4767 4767 g, t dbSNP:210517944

Target ORF information:

RefSeq Version NM_001113224
Organism Bos taurus (cattle)
Definition Bos taurus collagen, type II, alpha 1 (COL2A1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001113224

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCGGCAA CCAGGACCAA AGGGACAGAA AGGAGAACCT
GGAGATATCA AGGACATCGT AGGACCCAAG GGACCTCCTG GACCTCAGGG ACCCGCAGGT
GAACAAGGAC CCAGAGGTGA TCGTGGTGAC AAAGGTGAAA AAGGTGCTCC TGGACCTCGT
GGCAGAGATG GAGAGCCTGG GACCCCTGGA AACCCTGGCC CCCCTGGTCC TCCCGGCCCC
CCTGGCCCCC CTGGCCTTGG TGGAAACTTT GCTGCCCAGA TGGCTGGTGG ATTTGATGAG
AAGGCTGGTG GCGCCCAGAT GGGAGTAATG CAGGGACCAA TGGGCCCCAT GGGACCTCGG
GGACCCCCAG GCCCTGCTGG TGCTCCCGGA CCTCAAGGAT TTCAAGGCAA CCCTGGTGAA
CCTGGTGAAC CCGGTGTTTC TGGTCCCATG GGCCCCCGTG GTCCTCCTGG CCCCCCTGGG
AAACCTGGTG ATGATGGTGA AGCTGGAAAG CCTGGAAAAT CTGGCGAGAG AGGCCCTCCT
GGCCCTCAGG GTGCTCGTGG TTTCCCAGGA ACCCCAGGCC TTCCTGGTGT CAAAGGTCAC
AGAGGTTACC CAGGTCTAGA TGGTGCTAAG GGAGAAGCTG GTGCTCCAGG TGTGAAGGGT
GAGAGTGGTT CCCCAGGTGA GAACGGTTCT CCAGGCCCAA TGGGTCCCCG CGGCTTGCCT
GGTGAGAGAG GACGGACTGG CCCTGCCGGC GCTGCAGGTG CTCGAGGCAA TGACGGTCAG
CCAGGCCCTG CAGGGCCTCC GGGTCCTGTG GGTCCTGCTG GCGGTCCTGG CTTTCCTGGT
GCTCCTGGTG CCAAGGGTGA AGCTGGCCCC ACCGGTGCTC GAGGTCCCGA AGGCGCCCAG
GGTCCTCGCG GTGAACCGGG TACTCCTGGG TCCCCTGGGC CAGCTGGTGC TGCTGGCAAC
CCTGGAACTG ATGGAATCCC TGGAGCCAAG GGATCTGCTG GTGCCCCTGG CATTGCTGGT
GCTCCCGGCT TCCCTGGACC CCGTGGTCCA CCCGGCCCTC AAGGTGCAAC TGGTCCTCTG
GGCCCAAAAG GTCAAACGGG TGAGCCTGGT ATTGCTGGCT TCAAAGGCGA ACAAGGCCCC
AAGGGAGAAC CGGGCCCTGC TGGTCCCCAA GGAGCCCCTG GTCCTGCTGG TGAAGAAGGG
AAAAGAGGTG CCCGTGGAGA ACCTGGTGGT GCTGGGCCCG CCGGTCCCCC TGGAGAAAGA
GGCGCTCCTG GAAACCGTGG TTTCCCAGGT CAGGATGGTC TGGCAGGTCC CAAGGGAGCC
CCTGGAGAGC GAGGACCCAG TGGCCTCGCT GGTCCCAAAG GCGCCAACGG TGACCCTGGC
CGTCCCGGAG AGCCTGGCCT TCCTGGAGCC CGGGGTCTCA CTGGTCGCCC TGGTGATGCT
GGTCCTCAAG GCAAAGTTGG TCCTTCCGGA GCCCCTGGTG AAGACGGTCG CCCTGGACCC
CCAGGTCCTC AGGGGGCTCG TGGGCAGCCT GGTGTCATGG GTTTCCCTGG CCCCAAAGGT
GCCAATGGTG AGCCTGGCAA AGCTGGTGAG AAAGGACTGC CTGGTGCTCC TGGTCTGAGA
GGTCTTCCTG GCAAAGATGG TGAGACAGGT GCTGCAGGCC CCCCTGGACC CGCTGGACCA
GCTGGTGAAC GAGGCGAGCA GGGTGCTCCT GGGCCATCTG GGTTCCAGGG ACTTCCTGGC
CCTCCCGGCC CCCCAGGTGA AGGTGGAAAA CCAGGTGACC AGGGTGTTCC TGGTGAAGCT
GGAGCTCCCG GCCTCGTGGG TCCCAGGGGT GAACGAGGTT TCCCAGGAGA ACGTGGCTCC
CCCGGTTCCC AGGGCCTCCA GGGGGCCCGC GGTCTCCCCG GCACTCCTGG CACTGATGGT
CCCAAAGGCG CAGCTGGCCC AGCTGGCCCC CCTGGGGCTC AGGGCCCTCC AGGTCTGCAG
GGCATGCCCG GTGAGAGGGG AGCAGCTGGT ATCGCCGGAC CCAAGGGAGA CAGGGGTGAT
GTTGGTGAGA AAGGCCCCGA GGGCGCCCCC GGGAAGGACG GTGGACGAGG TCTGACTGGC
CCCATTGGCC CCCCTGGCCC AGCCGGAGCC AACGGTGAGA AGGGAGAAGT TGGACCCCCT
GGTCCTGCTG GAACTGCTGG TGCTCGAGGT GCCCCGGGTG AACGTGGAGA GACAGGCCCC
CCTGGACCTG CTGGATTCGC CGGTCCCCCT GGTGCTGATG GCCAGCCTGG TGCCAAAGGT
GAACAAGGAG AAGCCGGCCA GAAAGGTGAC GCTGGTGCCC CCGGTCCTCA GGGCCCCTCT
GGAGCTCCTG GGCCTCAGGG TCCTACTGGT GTGACCGGTC CTAAAGGAGC CCGAGGTGCT
CAAGGGCCCC CGGGAGCCAC CGGATTCCCC GGAGCAGCTG GCCGCGTCGG ACCCCCAGGC
TCCAATGGCA ACCCTGGACC CCCTGGTCCT CCTGGTCCTT CTGGAAAAGA TGGTCCTAAA
GGTGCTCGAG GCGACAGTGG GCCCCCTGGC CGAGCTGGTG ACCCTGGCCT CCAAGGTCCT
GCTGGACCCC CTGGCGAGAA GGGAGAGCCT GGAGATGATG GTCCTTCTGG TCCCGATGGT
CCTCCAGGTC CTCAGGGTCT GGCTGGTCAG AGGGGCATCG TTGGTCTGCC TGGACAGCGT
GGTGAGCGAG GATTCCCCGG CCTCCCCGGC CCCTCGGGTG AGCCTGGCAA GCAGGGAGCC
CCTGGAGCAT CTGGAGACCG AGGTCCCCCT GGCCCTGTGG GTCCTCCTGG CCTGACTGGT
CCTGCTGGTG AGCCTGGACG TGAGGGAAGC CCCGGTGCTG ACGGCCCCCC TGGCAGAGAT
GGCGCAGCTG GAGTCAAAGG TGACCGTGGA GAGACCGGTG CCGTGGGTGC TCCTGGAGCC
CCTGGGCCCC CTGGCTCTCC TGGCCCCGCT GGCCCAATTG GCAAGCAGGG AGACCGAGGA
GAAGCTGGCG CACAAGGTCC CATGGGACCT GCAGGACCTG CTGGAGCCCG GGGAATGCCA
GGCCCTCAAG GCCCCCGAGG TGACAAAGGA GAAACTGGAG AGGCTGGCGA GAGGGGACTG
AAGGGACACC GTGGCTTCAC TGGTCTGCAG GGTCTGCCCG GCCCCCCTGG TCCTTCTGGA
GACCAGGGTG CTTCCGGTCC TGCAGGTCCT TCCGGCCCCA GAGGTCCTCC TGGTCCCGTC
GGTCCCTCTG GCAAAGATGG TGCTAACGGA ATCCCTGGCC CCATTGGACC TCCTGGGCCC
CGTGGACGTT CCGGCGAGAC TGGCCCTGCT GGTCCTCCTG GAAACCCTGG ACCCCCTGGC
CCTCCTGGCC CCCCCGGCCC TGGCATCGAC ATGTCCGCCT TTGCTGGCCT TGGCCAGAGA
GAGAAAGGCC CCGATCCTCT GCAGTACATG CGGGCTGACG AGGCAGCCGG CAACCTGAGA
CAGCATGATG CCGAGGTGGA CGCCACACTC AAGTCCCTCA ACAACCAGAT CGAGAGCCTC
CGTAGCCCCG AGGGCTCCCG CAAGAACCCA GCTCGCACCT GCCGAGACCT GAAACTCTGC
CACCCTGAGT GGAAGAGCGG AGACTACTGG ATCGACCCCA ACCAGGGCTG CACCCTGGAT
GCCATGAAGG TTTTCTGCAA CATGGAGACT GGCGAGACCT GCGTCTACCC CAACCCGGCC
AGCGTCCCCA AGAAGAACTG GTGGAGCAGC AAGAGCAAGG ACAAGAAACA CATCTGGTTT
GGAGAAACCA TCAACGGTGG CTTCCACTTC AGCTATGGAG ATGACAACCT GGCTCCCAAC
ACCGCCAACG TCCAGATGAC CTTCCTGCGC CTGCTGTCCA CCGAGGGCTC TCAGAACATC
ACCTACCACT GCAAGAACAG CATTGCCTAC CTGGACGAAG CTGCTGGCAA CCTCAAGAAG
GCTCTGCTCA TCCAGGGCTC CAACGACGTG GAGATCCGGG CTGAGGGCAA CAGCAGGTTC
ACATATACCG TTCTGAAGGA TGGCTGCACG AAACACACCG GTAAGTGGGG CAAGACTATG
ATCGAGTACC GGTCACAGAA GACCTCCCGT CTGCCCATCA TTGACATTGC ACCCATGGAC
ATAGGAGGGC CCGAGCAGGA ATTCGGTGTG GACATAGGGC CTGTCTGCTT CTTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Definition of the native and denatured type II collagen binding site for fibronectin using a recombinant collagen system
J. Biol. Chem. 289 (8), 4941-4951 (2014)
An B, Abbonante V, Yigit S, Balduini A, Kaplan DL and Brodsky B.


book

Two subsystems of meniscal collagen and their different thermal stabilities
Dokl. Biochem. Biophys. 444, 175-177 (2012)
Ignatieva NY, Zakharkina OL, Lunin VV, Baratova LA, Grokhovskaya TE, Balalaeva IV and Sergeeva EA.


book

Disc strain and resulting positive mRNA expression from application of a noninvasive treatment
Spine 36 (14), E921-E928 (2011)
Desmoulin GT, Hewitt CR and Hunter CJ.


book

Free axial vibrations at 0 to 200 Hz positively affect extracellular matrix messenger ribonucleic acid expression in bovine nucleus pulposi
Spine 35 (15), 1437-1444 (2010)
Desmoulin GT, Reno CR and Hunter CJ.


book

Type II and VI collagen in nasal and articular cartilage and the effect of IL-1alpha on the distribution of these collagens
J. Mol. Histol. 41 (1), 9-17 (2010)
Jansen ID, Hollander AP, Buttle DJ and Everts V.


book

Covalent structure of collagen. Amino acid sequence of an arthritogenic cyanogen bromide peptide from type II collagen of bovine cartilage
Eur. J. Biochem. 181 (1), 159-173 (1989)
Seyer JM, Hasty KA and Kang AH.


book

Analysis of cDNA and genomic clones coding for the pro alpha 1 chain of calf type II collagen
Nucleic Acids Res. 13 (8), 2815-2826 (1985)
Sangiorgi,F.O., Benson-Chanda,V., de Wet,W.J., Sobel,M.E. and Ramirez,F.


book

The covalent structure of cartilage collagen. Evidence for sequence heterogeneity of bovine alpha1(II) chains
J. Biol. Chem. 252 (2), 639-643 (1977)
Butler,W.T., Finch,J.E. Jr. and Miller,E.J.


book

The covalent structure of cartilage collagen. Amino acid sequence of the NH2-terminal helical portion of the alpha 1 (II) chain
Biochemistry 15 (14), 3000-3006 (1976)
Butler,W.T., Miller,E.J. and Finch,J.E. Jr.


book

Isolation and characterization of the cyanogen bromide peptides from the alpha 1(II) chain of bovine and human cartilage collagen
Biochemistry 12 (17), 3153-3159 (1973)
Miller,E.J. and Lunde,L.G.


 
*
*
*
*