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COL2A1 collagen, type II, alpha 1 [Bos taurus (cattle)]

Gene Symbol COL2A1
Entrez Gene ID 407142
Full Name collagen, type II, alpha 1
Synonyms CB11
General protein information
Preferred Names
collagen alpha-1(II) chain
Names
collagen alpha-1(II) chain
pro-alpha 1(II)
cyanogen bromide
alpha-1 type II collagen
Gene Type protein-coding
Organism Bos taurus (cattle)
Genome

5

Summary lac of sum
Disorder MIM:

Disorder Html:





The following COL2A1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL2A1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OBa01441 NM_001001135 Bos taurus collagen, type II, alpha 1 (COL2A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 Quote Price
OBa01444 NM_001113224 Bos taurus collagen, type II, alpha 1 (COL2A1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OBa01441
Accession Version NM_001001135.2
Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-JAN-2015
Organism Bos taurus (cattle)
Product collagen alpha-1(II) chain isoform 1 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03017085.1, AAFC03017082.1 and AAFC03056593.1. On Jan 1, 2008 this sequence version replaced gi:47564047. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment of the human homolog (NM_001844.4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093753, SAMN02404548 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCAGAAG GCTGGCAGCT GTGTGCAGGA CGGGCAGAGG
TATAATGATA AGGATGTGTG GAAGCCCGAG CCCTGCCGGA TCTGTGTCTG TGACACTGGG
ACTGTCCTCT GCGACGACAT AATCTGTGAA GACATGAAAG ACTGCCTCAG CCCCGAGACC
CCCTTCGGAG AGTGCTGCCC CATCTGCTCA GCTGACCTCC CCACTGCCAG TGGGCAACCA
GGACCAAAGG GACAGAAAGG AGAACCTGGA GATATCAAGG ACATCGTAGG ACCCAAGGGA
CCTCCTGGAC CTCAGGGACC CGCAGGTGAA CAAGGACCCA GAGGTGATCG TGGTGACAAA
GGTGAAAAAG GTGCTCCTGG ACCTCGTGGC AGAGATGGAG AGCCTGGGAC CCCTGGAAAC
CCTGGCCCCC CTGGTCCTCC CGGCCCCCCT GGCCCCCCTG GCCTTGGTGG AAACTTTGCT
GCCCAGATGG CTGGTGGATT TGATGAGAAG GCTGGTGGCG CCCAGATGGG AGTAATGCAG
GGACCAATGG GCCCCATGGG ACCTCGGGGA CCCCCAGGCC CTGCTGGTGC TCCCGGACCT
CAAGGATTTC AAGGCAACCC TGGTGAACCT GGTGAACCCG GTGTTTCTGG TCCCATGGGC
CCCCGTGGTC CTCCTGGCCC CCCTGGGAAA CCTGGTGATG ATGGTGAAGC TGGAAAGCCT
GGAAAATCTG GCGAGAGAGG CCCTCCTGGC CCTCAGGGTG CTCGTGGTTT CCCAGGAACC
CCAGGCCTTC CTGGTGTCAA AGGTCACAGA GGTTACCCAG GTCTAGATGG TGCTAAGGGA
GAAGCTGGTG CTCCAGGTGT GAAGGGTGAG AGTGGTTCCC CAGGTGAGAA CGGTTCTCCA
GGCCCAATGG GTCCCCGCGG CTTGCCTGGT GAGAGAGGAC GGACTGGCCC TGCCGGCGCT
GCAGGTGCTC GAGGCAATGA CGGTCAGCCA GGCCCTGCAG GGCCTCCGGG TCCTGTGGGT
CCTGCTGGCG GTCCTGGCTT TCCTGGTGCT CCTGGTGCCA AGGGTGAAGC TGGCCCCACC
GGTGCTCGAG GTCCCGAAGG CGCCCAGGGT CCTCGCGGTG AACCGGGTAC TCCTGGGTCC
CCTGGGCCAG CTGGTGCTGC TGGCAACCCT GGAACTGATG GAATCCCTGG AGCCAAGGGA
TCTGCTGGTG CCCCTGGCAT TGCTGGTGCT CCCGGCTTCC CTGGACCCCG TGGTCCACCC
GGCCCTCAAG GTGCAACTGG TCCTCTGGGC CCAAAAGGTC AAACGGGTGA GCCTGGTATT
GCTGGCTTCA AAGGCGAACA AGGCCCCAAG GGAGAACCGG GCCCTGCTGG TCCCCAAGGA
GCCCCTGGTC CTGCTGGTGA AGAAGGGAAA AGAGGTGCCC GTGGAGAACC TGGTGGTGCT
GGGCCCGCCG GTCCCCCTGG AGAAAGAGGC GCTCCTGGAA ACCGTGGTTT CCCAGGTCAG
GATGGTCTGG CAGGTCCCAA GGGAGCCCCT GGAGAGCGAG GACCCAGTGG CCTCGCTGGT
CCCAAAGGCG CCAACGGTGA CCCTGGCCGT CCCGGAGAGC CTGGCCTTCC TGGAGCCCGG
GGTCTCACTG GTCGCCCTGG TGATGCTGGT CCTCAAGGCA AAGTTGGTCC TTCCGGAGCC
CCTGGTGAAG ACGGTCGCCC TGGACCCCCA GGTCCTCAGG GGGCTCGTGG GCAGCCTGGT
GTCATGGGTT TCCCTGGCCC CAAAGGTGCC AATGGTGAGC CTGGCAAAGC TGGTGAGAAA
GGACTGCCTG GTGCTCCTGG TCTGAGAGGT CTTCCTGGCA AAGATGGTGA GACAGGTGCT
GCAGGCCCCC CTGGACCCGC TGGACCAGCT GGTGAACGAG GCGAGCAGGG TGCTCCTGGG
CCATCTGGGT TCCAGGGACT TCCTGGCCCT CCCGGCCCCC CAGGTGAAGG TGGAAAACCA
GGTGACCAGG GTGTTCCTGG TGAAGCTGGA GCTCCCGGCC TCGTGGGTCC CAGGGGTGAA
CGAGGTTTCC CAGGAGAACG TGGCTCCCCC GGTTCCCAGG GCCTCCAGGG GGCCCGCGGT
CTCCCCGGCA CTCCTGGCAC TGATGGTCCC AAAGGCGCAG CTGGCCCAGC TGGCCCCCCT
GGGGCTCAGG GCCCTCCAGG TCTGCAGGGC ATGCCCGGTG AGAGGGGAGC AGCTGGTATC
GCCGGACCCA AGGGAGACAG GGGTGATGTT GGTGAGAAAG GCCCCGAGGG CGCCCCCGGG
AAGGACGGTG GACGAGGTCT GACTGGCCCC ATTGGCCCCC CTGGCCCAGC CGGAGCCAAC
GGTGAGAAGG GAGAAGTTGG ACCCCCTGGT CCTGCTGGAA CTGCTGGTGC TCGAGGTGCC
CCGGGTGAAC GTGGAGAGAC AGGCCCCCCT GGACCTGCTG GATTCGCCGG TCCCCCTGGT
GCTGATGGCC AGCCTGGTGC CAAAGGTGAA CAAGGAGAAG CCGGCCAGAA AGGTGACGCT
GGTGCCCCCG GTCCTCAGGG CCCCTCTGGA GCTCCTGGGC CTCAGGGTCC TACTGGTGTG
ACCGGTCCTA AAGGAGCCCG AGGTGCTCAA GGGCCCCCGG GAGCCACCGG ATTCCCCGGA
GCAGCTGGCC GCGTCGGACC CCCAGGCTCC AATGGCAACC CTGGACCCCC TGGTCCTCCT
GGTCCTTCTG GAAAAGATGG TCCTAAAGGT GCTCGAGGCG ACAGTGGGCC CCCTGGCCGA
GCTGGTGACC CTGGCCTCCA AGGTCCTGCT GGACCCCCTG GCGAGAAGGG AGAGCCTGGA
GATGATGGTC CTTCTGGTCC CGATGGTCCT CCAGGTCCTC AGGGTCTGGC TGGTCAGAGG
GGCATCGTTG GTCTGCCTGG ACAGCGTGGT GAGCGAGGAT TCCCCGGCCT CCCCGGCCCC
TCGGGTGAGC CTGGCAAGCA GGGAGCCCCT GGAGCATCTG GAGACCGAGG TCCCCCTGGC
CCTGTGGGTC CTCCTGGCCT GACTGGTCCT GCTGGTGAGC CTGGACGTGA GGGAAGCCCC
GGTGCTGACG GCCCCCCTGG CAGAGATGGC GCAGCTGGAG TCAAAGGTGA CCGTGGAGAG
ACCGGTGCCG TGGGTGCTCC TGGAGCCCCT GGGCCCCCTG GCTCTCCTGG CCCCGCTGGC
CCAATTGGCA AGCAGGGAGA CCGAGGAGAA GCTGGCGCAC AAGGTCCCAT GGGACCTGCA
GGACCTGCTG GAGCCCGGGG AATGCCAGGC CCTCAAGGCC CCCGAGGTGA CAAAGGAGAA
ACTGGAGAGG CTGGCGAGAG GGGACTGAAG GGACACCGTG GCTTCACTGG TCTGCAGGGT
CTGCCCGGCC CCCCTGGTCC TTCTGGAGAC CAGGGTGCTT CCGGTCCTGC AGGTCCTTCC
GGCCCCAGAG GTCCTCCTGG TCCCGTCGGT CCCTCTGGCA AAGATGGTGC TAACGGAATC
CCTGGCCCCA TTGGACCTCC TGGGCCCCGT GGACGTTCCG GCGAGACTGG CCCTGCTGGT
CCTCCTGGAA ACCCTGGACC CCCTGGCCCT CCTGGCCCCC CCGGCCCTGG CATCGACATG
TCCGCCTTTG CTGGCCTTGG CCAGAGAGAG AAAGGCCCCG ATCCTCTGCA GTACATGCGG
GCTGACGAGG CAGCCGGCAA CCTGAGACAG CATGATGCCG AGGTGGACGC CACACTCAAG
TCCCTCAACA ACCAGATCGA GAGCCTCCGT AGCCCCGAGG GCTCCCGCAA GAACCCAGCT
CGCACCTGCC GAGACCTGAA ACTCTGCCAC CCTGAGTGGA AGAGCGGAGA CTACTGGATC
GACCCCAACC AGGGCTGCAC CCTGGATGCC ATGAAGGTTT TCTGCAACAT GGAGACTGGC
GAGACCTGCG TCTACCCCAA CCCGGCCAGC GTCCCCAAGA AGAACTGGTG GAGCAGCAAG
AGCAAGGACA AGAAACACAT CTGGTTTGGA GAAACCATCA ACGGTGGCTT CCACTTCAGC
TATGGAGATG ACAACCTGGC TCCCAACACC GCCAACGTCC AGATGACCTT CCTGCGCCTG
CTGTCCACCG AGGGCTCTCA GAACATCACC TACCACTGCA AGAACAGCAT TGCCTACCTG
GACGAAGCTG CTGGCAACCT CAAGAAGGCT CTGCTCATCC AGGGCTCCAA CGACGTGGAG
ATCCGGGCTG AGGGCAACAG CAGGTTCACA TATACCGTTC TGAAGGATGG CTGCACGAAA
CACACCGGTA AGTGGGGCAA GACTATGATC GAGTACCGGT CACAGAAGAC CTCCCGTCTG
CCCATCATTG ACATTGCACC CATGGACATA GGAGGGCCCG AGCAGGAATT CGGTGTGGAC
ATAGGGCCTG TCTGCTTCTT GTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001001135.2
CDS27..4490
Misc Feature(1)21..23(+)
Misc Feature(2)126..293(+)
Misc Feature(3)567..572(+)
Misc Feature(4)627..3668(+)
Misc Feature(5)660..662(+)
Misc Feature(6)678..680(+)
Misc Feature(7)714..716(+)
Misc Feature(8)723..725(+)
Misc Feature(9)759..761(+)
Misc Feature(10)768..770(+)
Misc Feature(11)777..779(+)
Misc Feature(12)798..977(+)
Misc Feature(13)804..806(+)
Misc Feature(14)831..833(+)
Misc Feature(15)858..860(+)
Misc Feature(16)867..869(+)
Misc Feature(17)876..878(+)
Misc Feature(18)885..887(+)
Misc Feature(19)903..905(+)
Misc Feature(20)921..923(+)
Misc Feature(21)939..941(+)
Misc Feature(22)948..950(+)
Misc Feature(23)966..968(+)
Misc Feature(24)984..986(+)
Misc Feature(25)1011..1013(+)
Misc Feature(26)1074..1076(+)
Misc Feature(27)1092..1094(+)
Misc Feature(28)1119..1121(+)
Misc Feature(29)1128..1130(+)
Misc Feature(30)1137..1139(+)
Misc Feature(31)1146..1148(+)
Misc Feature(32)1149..1301(+)
Misc Feature(33)1209..1211(+)
Misc Feature(34)1218..1220(+)
Misc Feature(35)1227..1229(+)
Misc Feature(36)1254..1256(+)
Misc Feature(37)1272..1274(+)
Misc Feature(38)1281..1283(+)
Misc Feature(39)1299..1301(+)
Misc Feature(40)1317..1319(+)
Misc Feature(41)1326..1328(+)
Misc Feature(42)1344..1346(+)
Misc Feature(43)1365..1505(+)
Misc Feature(44)1380..1382(+)
Misc Feature(45)1398..1400(+)
Misc Feature(46)1416..1418(+)
Misc Feature(47)1434..1436(+)
Misc Feature(48)1443..1445(+)
Misc Feature(49)1470..1472(+)
Misc Feature(50)1515..1517(+)
Misc Feature(51)1542..1544(+)
Misc Feature(52)1551..1706(+)
Misc Feature(53)1560..1562(+)
Misc Feature(54)1578..1580(+)
Misc Feature(55)1605..1607(+)
Misc Feature(56)1614..1616(+)
Misc Feature(57)1650..1652(+)
Misc Feature(58)1662..1877(+)
Misc Feature(59)1677..1679(+)
Misc Feature(60)1695..1697(+)
Misc Feature(61)1722..1724(+)
Misc Feature(62)1767..1769(+)
Misc Feature(63)1785..1787(+)
Misc Feature(64)1794..1796(+)
Misc Feature(65)1821..1823(+)
Misc Feature(66)1839..1841(+)
Misc Feature(67)1848..1850(+)
Misc Feature(68)1866..1868(+)
Misc Feature(69)1884..1886(+)
Misc Feature(70)1893..1895(+)
Misc Feature(71)1902..1904(+)
Misc Feature(72)1920..1922(+)
Misc Feature(73)1956..1958(+)
Misc Feature(74)2001..2003(+)
Misc Feature(75)2028..2030(+)
Misc Feature(76)2037..2039(+)
Misc Feature(77)2046..2048(+)
Misc Feature(78)2076..>2204(+)
Misc Feature(79)2427..2606(+)
Misc Feature(80)2535..2711(+)
Misc Feature(81)2832..3044(+)
Misc Feature(82)3279..3410(+)
Misc Feature(83)3669..3749(+)
Misc Feature(84)3747..3752(+)
Misc Feature(85)3780..4487(+)
Translation
Position Chain Variation Link
103 103 a, g dbSNP:134559128
1508 1508 g, t dbSNP:110274310
2156 2156 c, t dbSNP:108981059
2402 2402 c, t dbSNP:209731964
2719 2719 a, c dbSNP:109700727
3236 3236 c, t dbSNP:384560704
3719 3719 a, g dbSNP:382996583
3749 3749 c, t dbSNP:110943643
3836 3836 c, t dbSNP:136487089
4819 4819 g, t dbSNP:210517944
4936 4936 g, t dbSNP:133539847

Target ORF information:

RefSeq Version NM_001001135
Organism Bos taurus (cattle)
Definition Bos taurus collagen, type II, alpha 1 (COL2A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001001135

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCAGAAG GCTGGCAGCT GTGTGCAGGA CGGGCAGAGG
TATAATGATA AGGATGTGTG GAAGCCCGAG CCCTGCCGGA TCTGTGTCTG TGACACTGGG
ACTGTCCTCT GCGACGACAT AATCTGTGAA GACATGAAAG ACTGCCTCAG CCCCGAGACC
CCCTTCGGAG AGTGCTGCCC CATCTGCTCA GCTGACCTCC CCACTGCCAG TGGGCAACCA
GGACCAAAGG GACAGAAAGG AGAACCTGGA GATATCAAGG ACATCGTAGG ACCCAAGGGA
CCTCCTGGAC CTCAGGGACC CGCAGGTGAA CAAGGACCCA GAGGTGATCG TGGTGACAAA
GGTGAAAAAG GTGCTCCTGG ACCTCGTGGC AGAGATGGAG AGCCTGGGAC CCCTGGAAAC
CCTGGCCCCC CTGGTCCTCC CGGCCCCCCT GGCCCCCCTG GCCTTGGTGG AAACTTTGCT
GCCCAGATGG CTGGTGGATT TGATGAGAAG GCTGGTGGCG CCCAGATGGG AGTAATGCAG
GGACCAATGG GCCCCATGGG ACCTCGGGGA CCCCCAGGCC CTGCTGGTGC TCCCGGACCT
CAAGGATTTC AAGGCAACCC TGGTGAACCT GGTGAACCCG GTGTTTCTGG TCCCATGGGC
CCCCGTGGTC CTCCTGGCCC CCCTGGGAAA CCTGGTGATG ATGGTGAAGC TGGAAAGCCT
GGAAAATCTG GCGAGAGAGG CCCTCCTGGC CCTCAGGGTG CTCGTGGTTT CCCAGGAACC
CCAGGCCTTC CTGGTGTCAA AGGTCACAGA GGTTACCCAG GTCTAGATGG TGCTAAGGGA
GAAGCTGGTG CTCCAGGTGT GAAGGGTGAG AGTGGTTCCC CAGGTGAGAA CGGTTCTCCA
GGCCCAATGG GTCCCCGCGG CTTGCCTGGT GAGAGAGGAC GGACTGGCCC TGCCGGCGCT
GCAGGTGCTC GAGGCAATGA CGGTCAGCCA GGCCCTGCAG GGCCTCCGGG TCCTGTGGGT
CCTGCTGGCG GTCCTGGCTT TCCTGGTGCT CCTGGTGCCA AGGGTGAAGC TGGCCCCACC
GGTGCTCGAG GTCCCGAAGG CGCCCAGGGT CCTCGCGGTG AACCGGGTAC TCCTGGGTCC
CCTGGGCCAG CTGGTGCTGC TGGCAACCCT GGAACTGATG GAATCCCTGG AGCCAAGGGA
TCTGCTGGTG CCCCTGGCAT TGCTGGTGCT CCCGGCTTCC CTGGACCCCG TGGTCCACCC
GGCCCTCAAG GTGCAACTGG TCCTCTGGGC CCAAAAGGTC AAACGGGTGA GCCTGGTATT
GCTGGCTTCA AAGGCGAACA AGGCCCCAAG GGAGAACCGG GCCCTGCTGG TCCCCAAGGA
GCCCCTGGTC CTGCTGGTGA AGAAGGGAAA AGAGGTGCCC GTGGAGAACC TGGTGGTGCT
GGGCCCGCCG GTCCCCCTGG AGAAAGAGGC GCTCCTGGAA ACCGTGGTTT CCCAGGTCAG
GATGGTCTGG CAGGTCCCAA GGGAGCCCCT GGAGAGCGAG GACCCAGTGG CCTCGCTGGT
CCCAAAGGCG CCAACGGTGA CCCTGGCCGT CCCGGAGAGC CTGGCCTTCC TGGAGCCCGG
GGTCTCACTG GTCGCCCTGG TGATGCTGGT CCTCAAGGCA AAGTTGGTCC TTCCGGAGCC
CCTGGTGAAG ACGGTCGCCC TGGACCCCCA GGTCCTCAGG GGGCTCGTGG GCAGCCTGGT
GTCATGGGTT TCCCTGGCCC CAAAGGTGCC AATGGTGAGC CTGGCAAAGC TGGTGAGAAA
GGACTGCCTG GTGCTCCTGG TCTGAGAGGT CTTCCTGGCA AAGATGGTGA GACAGGTGCT
GCAGGCCCCC CTGGACCCGC TGGACCAGCT GGTGAACGAG GCGAGCAGGG TGCTCCTGGG
CCATCTGGGT TCCAGGGACT TCCTGGCCCT CCCGGCCCCC CAGGTGAAGG TGGAAAACCA
GGTGACCAGG GTGTTCCTGG TGAAGCTGGA GCTCCCGGCC TCGTGGGTCC CAGGGGTGAA
CGAGGTTTCC CAGGAGAACG TGGCTCCCCC GGTTCCCAGG GCCTCCAGGG GGCCCGCGGT
CTCCCCGGCA CTCCTGGCAC TGATGGTCCC AAAGGCGCAG CTGGCCCAGC TGGCCCCCCT
GGGGCTCAGG GCCCTCCAGG TCTGCAGGGC ATGCCCGGTG AGAGGGGAGC AGCTGGTATC
GCCGGACCCA AGGGAGACAG GGGTGATGTT GGTGAGAAAG GCCCCGAGGG CGCCCCCGGG
AAGGACGGTG GACGAGGTCT GACTGGCCCC ATTGGCCCCC CTGGCCCAGC CGGAGCCAAC
GGTGAGAAGG GAGAAGTTGG ACCCCCTGGT CCTGCTGGAA CTGCTGGTGC TCGAGGTGCC
CCGGGTGAAC GTGGAGAGAC AGGCCCCCCT GGACCTGCTG GATTCGCCGG TCCCCCTGGT
GCTGATGGCC AGCCTGGTGC CAAAGGTGAA CAAGGAGAAG CCGGCCAGAA AGGTGACGCT
GGTGCCCCCG GTCCTCAGGG CCCCTCTGGA GCTCCTGGGC CTCAGGGTCC TACTGGTGTG
ACCGGTCCTA AAGGAGCCCG AGGTGCTCAA GGGCCCCCGG GAGCCACCGG ATTCCCCGGA
GCAGCTGGCC GCGTCGGACC CCCAGGCTCC AATGGCAACC CTGGACCCCC TGGTCCTCCT
GGTCCTTCTG GAAAAGATGG TCCTAAAGGT GCTCGAGGCG ACAGTGGGCC CCCTGGCCGA
GCTGGTGACC CTGGCCTCCA AGGTCCTGCT GGACCCCCTG GCGAGAAGGG AGAGCCTGGA
GATGATGGTC CTTCTGGTCC CGATGGTCCT CCAGGTCCTC AGGGTCTGGC TGGTCAGAGG
GGCATCGTTG GTCTGCCTGG ACAGCGTGGT GAGCGAGGAT TCCCCGGCCT CCCCGGCCCC
TCGGGTGAGC CTGGCAAGCA GGGAGCCCCT GGAGCATCTG GAGACCGAGG TCCCCCTGGC
CCTGTGGGTC CTCCTGGCCT GACTGGTCCT GCTGGTGAGC CTGGACGTGA GGGAAGCCCC
GGTGCTGACG GCCCCCCTGG CAGAGATGGC GCAGCTGGAG TCAAAGGTGA CCGTGGAGAG
ACCGGTGCCG TGGGTGCTCC TGGAGCCCCT GGGCCCCCTG GCTCTCCTGG CCCCGCTGGC
CCAATTGGCA AGCAGGGAGA CCGAGGAGAA GCTGGCGCAC AAGGTCCCAT GGGACCTGCA
GGACCTGCTG GAGCCCGGGG AATGCCAGGC CCTCAAGGCC CCCGAGGTGA CAAAGGAGAA
ACTGGAGAGG CTGGCGAGAG GGGACTGAAG GGACACCGTG GCTTCACTGG TCTGCAGGGT
CTGCCCGGCC CCCCTGGTCC TTCTGGAGAC CAGGGTGCTT CCGGTCCTGC AGGTCCTTCC
GGCCCCAGAG GTCCTCCTGG TCCCGTCGGT CCCTCTGGCA AAGATGGTGC TAACGGAATC
CCTGGCCCCA TTGGACCTCC TGGGCCCCGT GGACGTTCCG GCGAGACTGG CCCTGCTGGT
CCTCCTGGAA ACCCTGGACC CCCTGGCCCT CCTGGCCCCC CCGGCCCTGG CATCGACATG
TCCGCCTTTG CTGGCCTTGG CCAGAGAGAG AAAGGCCCCG ATCCTCTGCA GTACATGCGG
GCTGACGAGG CAGCCGGCAA CCTGAGACAG CATGATGCCG AGGTGGACGC CACACTCAAG
TCCCTCAACA ACCAGATCGA GAGCCTCCGT AGCCCCGAGG GCTCCCGCAA GAACCCAGCT
CGCACCTGCC GAGACCTGAA ACTCTGCCAC CCTGAGTGGA AGAGCGGAGA CTACTGGATC
GACCCCAACC AGGGCTGCAC CCTGGATGCC ATGAAGGTTT TCTGCAACAT GGAGACTGGC
GAGACCTGCG TCTACCCCAA CCCGGCCAGC GTCCCCAAGA AGAACTGGTG GAGCAGCAAG
AGCAAGGACA AGAAACACAT CTGGTTTGGA GAAACCATCA ACGGTGGCTT CCACTTCAGC
TATGGAGATG ACAACCTGGC TCCCAACACC GCCAACGTCC AGATGACCTT CCTGCGCCTG
CTGTCCACCG AGGGCTCTCA GAACATCACC TACCACTGCA AGAACAGCAT TGCCTACCTG
GACGAAGCTG CTGGCAACCT CAAGAAGGCT CTGCTCATCC AGGGCTCCAA CGACGTGGAG
ATCCGGGCTG AGGGCAACAG CAGGTTCACA TATACCGTTC TGAAGGATGG CTGCACGAAA
CACACCGGTA AGTGGGGCAA GACTATGATC GAGTACCGGT CACAGAAGAC CTCCCGTCTG
CCCATCATTG ACATTGCACC CATGGACATA GGAGGGCCCG AGCAGGAATT CGGTGTGGAC
ATAGGGCCTG TCTGCTTCTT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa01444
Accession Version NM_001113224.1
Sequence Information ORF Nucleotide Sequence (Length: 4257bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 04-JAN-2015
Organism Bos taurus (cattle)
Product collagen alpha-1(II) chain isoform 2 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03017085.1, AAFC03017082.1 and AAFC03056593.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a shorter protein. The resulting protein (isoform 2) lacks the von Willebrand factor type C (VWC) domain, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignment of the human homolog (NM_033150.2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093753, SAMN02404548 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCGGCAA CCAGGACCAA AGGGACAGAA AGGAGAACCT
GGAGATATCA AGGACATCGT AGGACCCAAG GGACCTCCTG GACCTCAGGG ACCCGCAGGT
GAACAAGGAC CCAGAGGTGA TCGTGGTGAC AAAGGTGAAA AAGGTGCTCC TGGACCTCGT
GGCAGAGATG GAGAGCCTGG GACCCCTGGA AACCCTGGCC CCCCTGGTCC TCCCGGCCCC
CCTGGCCCCC CTGGCCTTGG TGGAAACTTT GCTGCCCAGA TGGCTGGTGG ATTTGATGAG
AAGGCTGGTG GCGCCCAGAT GGGAGTAATG CAGGGACCAA TGGGCCCCAT GGGACCTCGG
GGACCCCCAG GCCCTGCTGG TGCTCCCGGA CCTCAAGGAT TTCAAGGCAA CCCTGGTGAA
CCTGGTGAAC CCGGTGTTTC TGGTCCCATG GGCCCCCGTG GTCCTCCTGG CCCCCCTGGG
AAACCTGGTG ATGATGGTGA AGCTGGAAAG CCTGGAAAAT CTGGCGAGAG AGGCCCTCCT
GGCCCTCAGG GTGCTCGTGG TTTCCCAGGA ACCCCAGGCC TTCCTGGTGT CAAAGGTCAC
AGAGGTTACC CAGGTCTAGA TGGTGCTAAG GGAGAAGCTG GTGCTCCAGG TGTGAAGGGT
GAGAGTGGTT CCCCAGGTGA GAACGGTTCT CCAGGCCCAA TGGGTCCCCG CGGCTTGCCT
GGTGAGAGAG GACGGACTGG CCCTGCCGGC GCTGCAGGTG CTCGAGGCAA TGACGGTCAG
CCAGGCCCTG CAGGGCCTCC GGGTCCTGTG GGTCCTGCTG GCGGTCCTGG CTTTCCTGGT
GCTCCTGGTG CCAAGGGTGA AGCTGGCCCC ACCGGTGCTC GAGGTCCCGA AGGCGCCCAG
GGTCCTCGCG GTGAACCGGG TACTCCTGGG TCCCCTGGGC CAGCTGGTGC TGCTGGCAAC
CCTGGAACTG ATGGAATCCC TGGAGCCAAG GGATCTGCTG GTGCCCCTGG CATTGCTGGT
GCTCCCGGCT TCCCTGGACC CCGTGGTCCA CCCGGCCCTC AAGGTGCAAC TGGTCCTCTG
GGCCCAAAAG GTCAAACGGG TGAGCCTGGT ATTGCTGGCT TCAAAGGCGA ACAAGGCCCC
AAGGGAGAAC CGGGCCCTGC TGGTCCCCAA GGAGCCCCTG GTCCTGCTGG TGAAGAAGGG
AAAAGAGGTG CCCGTGGAGA ACCTGGTGGT GCTGGGCCCG CCGGTCCCCC TGGAGAAAGA
GGCGCTCCTG GAAACCGTGG TTTCCCAGGT CAGGATGGTC TGGCAGGTCC CAAGGGAGCC
CCTGGAGAGC GAGGACCCAG TGGCCTCGCT GGTCCCAAAG GCGCCAACGG TGACCCTGGC
CGTCCCGGAG AGCCTGGCCT TCCTGGAGCC CGGGGTCTCA CTGGTCGCCC TGGTGATGCT
GGTCCTCAAG GCAAAGTTGG TCCTTCCGGA GCCCCTGGTG AAGACGGTCG CCCTGGACCC
CCAGGTCCTC AGGGGGCTCG TGGGCAGCCT GGTGTCATGG GTTTCCCTGG CCCCAAAGGT
GCCAATGGTG AGCCTGGCAA AGCTGGTGAG AAAGGACTGC CTGGTGCTCC TGGTCTGAGA
GGTCTTCCTG GCAAAGATGG TGAGACAGGT GCTGCAGGCC CCCCTGGACC CGCTGGACCA
GCTGGTGAAC GAGGCGAGCA GGGTGCTCCT GGGCCATCTG GGTTCCAGGG ACTTCCTGGC
CCTCCCGGCC CCCCAGGTGA AGGTGGAAAA CCAGGTGACC AGGGTGTTCC TGGTGAAGCT
GGAGCTCCCG GCCTCGTGGG TCCCAGGGGT GAACGAGGTT TCCCAGGAGA ACGTGGCTCC
CCCGGTTCCC AGGGCCTCCA GGGGGCCCGC GGTCTCCCCG GCACTCCTGG CACTGATGGT
CCCAAAGGCG CAGCTGGCCC AGCTGGCCCC CCTGGGGCTC AGGGCCCTCC AGGTCTGCAG
GGCATGCCCG GTGAGAGGGG AGCAGCTGGT ATCGCCGGAC CCAAGGGAGA CAGGGGTGAT
GTTGGTGAGA AAGGCCCCGA GGGCGCCCCC GGGAAGGACG GTGGACGAGG TCTGACTGGC
CCCATTGGCC CCCCTGGCCC AGCCGGAGCC AACGGTGAGA AGGGAGAAGT TGGACCCCCT
GGTCCTGCTG GAACTGCTGG TGCTCGAGGT GCCCCGGGTG AACGTGGAGA GACAGGCCCC
CCTGGACCTG CTGGATTCGC CGGTCCCCCT GGTGCTGATG GCCAGCCTGG TGCCAAAGGT
GAACAAGGAG AAGCCGGCCA GAAAGGTGAC GCTGGTGCCC CCGGTCCTCA GGGCCCCTCT
GGAGCTCCTG GGCCTCAGGG TCCTACTGGT GTGACCGGTC CTAAAGGAGC CCGAGGTGCT
CAAGGGCCCC CGGGAGCCAC CGGATTCCCC GGAGCAGCTG GCCGCGTCGG ACCCCCAGGC
TCCAATGGCA ACCCTGGACC CCCTGGTCCT CCTGGTCCTT CTGGAAAAGA TGGTCCTAAA
GGTGCTCGAG GCGACAGTGG GCCCCCTGGC CGAGCTGGTG ACCCTGGCCT CCAAGGTCCT
GCTGGACCCC CTGGCGAGAA GGGAGAGCCT GGAGATGATG GTCCTTCTGG TCCCGATGGT
CCTCCAGGTC CTCAGGGTCT GGCTGGTCAG AGGGGCATCG TTGGTCTGCC TGGACAGCGT
GGTGAGCGAG GATTCCCCGG CCTCCCCGGC CCCTCGGGTG AGCCTGGCAA GCAGGGAGCC
CCTGGAGCAT CTGGAGACCG AGGTCCCCCT GGCCCTGTGG GTCCTCCTGG CCTGACTGGT
CCTGCTGGTG AGCCTGGACG TGAGGGAAGC CCCGGTGCTG ACGGCCCCCC TGGCAGAGAT
GGCGCAGCTG GAGTCAAAGG TGACCGTGGA GAGACCGGTG CCGTGGGTGC TCCTGGAGCC
CCTGGGCCCC CTGGCTCTCC TGGCCCCGCT GGCCCAATTG GCAAGCAGGG AGACCGAGGA
GAAGCTGGCG CACAAGGTCC CATGGGACCT GCAGGACCTG CTGGAGCCCG GGGAATGCCA
GGCCCTCAAG GCCCCCGAGG TGACAAAGGA GAAACTGGAG AGGCTGGCGA GAGGGGACTG
AAGGGACACC GTGGCTTCAC TGGTCTGCAG GGTCTGCCCG GCCCCCCTGG TCCTTCTGGA
GACCAGGGTG CTTCCGGTCC TGCAGGTCCT TCCGGCCCCA GAGGTCCTCC TGGTCCCGTC
GGTCCCTCTG GCAAAGATGG TGCTAACGGA ATCCCTGGCC CCATTGGACC TCCTGGGCCC
CGTGGACGTT CCGGCGAGAC TGGCCCTGCT GGTCCTCCTG GAAACCCTGG ACCCCCTGGC
CCTCCTGGCC CCCCCGGCCC TGGCATCGAC ATGTCCGCCT TTGCTGGCCT TGGCCAGAGA
GAGAAAGGCC CCGATCCTCT GCAGTACATG CGGGCTGACG AGGCAGCCGG CAACCTGAGA
CAGCATGATG CCGAGGTGGA CGCCACACTC AAGTCCCTCA ACAACCAGAT CGAGAGCCTC
CGTAGCCCCG AGGGCTCCCG CAAGAACCCA GCTCGCACCT GCCGAGACCT GAAACTCTGC
CACCCTGAGT GGAAGAGCGG AGACTACTGG ATCGACCCCA ACCAGGGCTG CACCCTGGAT
GCCATGAAGG TTTTCTGCAA CATGGAGACT GGCGAGACCT GCGTCTACCC CAACCCGGCC
AGCGTCCCCA AGAAGAACTG GTGGAGCAGC AAGAGCAAGG ACAAGAAACA CATCTGGTTT
GGAGAAACCA TCAACGGTGG CTTCCACTTC AGCTATGGAG ATGACAACCT GGCTCCCAAC
ACCGCCAACG TCCAGATGAC CTTCCTGCGC CTGCTGTCCA CCGAGGGCTC TCAGAACATC
ACCTACCACT GCAAGAACAG CATTGCCTAC CTGGACGAAG CTGCTGGCAA CCTCAAGAAG
GCTCTGCTCA TCCAGGGCTC CAACGACGTG GAGATCCGGG CTGAGGGCAA CAGCAGGTTC
ACATATACCG TTCTGAAGGA TGGCTGCACG AAACACACCG GTAAGTGGGG CAAGACTATG
ATCGAGTACC GGTCACAGAA GACCTCCCGT CTGCCCATCA TTGACATTGC ACCCATGGAC
ATAGGAGGGC CCGAGCAGGA ATTCGGTGTG GACATAGGGC CTGTCTGCTT CTTGTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001106695.1
CDS182..4438
Misc Feature(1)176..178(+)
Misc Feature(2)746..925(+)
Misc Feature(3)1097..1249(+)
Misc Feature(4)1313..1453(+)
Misc Feature(5)1499..1654(+)
Misc Feature(6)1610..1825(+)
Misc Feature(7)2024..>2152(+)
Misc Feature(8)2375..2554(+)
Misc Feature(9)2483..2659(+)
Misc Feature(10)2780..2992(+)
Misc Feature(11)3227..3358(+)
Misc Feature(12)3728..4435(+)
Translation
Position Chain Variation Link
258 258 a, g dbSNP:134559128
1456 1456 g, t dbSNP:110274310
2104 2104 c, t dbSNP:108981059
2350 2350 c, t dbSNP:209731964
2667 2667 a, c dbSNP:109700727
3184 3184 c, t dbSNP:384560704
3667 3667 a, g dbSNP:382996583
3697 3697 c, t dbSNP:110943643
3784 3784 c, t dbSNP:136487089
4767 4767 g, t dbSNP:210517944

Target ORF information:

RefSeq Version NM_001113224
Organism Bos taurus (cattle)
Definition Bos taurus collagen, type II, alpha 1 (COL2A1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001113224

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGATCCGCC TCGGGGCTCC CCAGACGCTG GTGCTGCTGA CGCTGCTCGT CGCCGCTGTC 
CTTCGGTGTC ACGGCCAGGA TGTCCGGCAA CCAGGACCAA AGGGACAGAA AGGAGAACCT
GGAGATATCA AGGACATCGT AGGACCCAAG GGACCTCCTG GACCTCAGGG ACCCGCAGGT
GAACAAGGAC CCAGAGGTGA TCGTGGTGAC AAAGGTGAAA AAGGTGCTCC TGGACCTCGT
GGCAGAGATG GAGAGCCTGG GACCCCTGGA AACCCTGGCC CCCCTGGTCC TCCCGGCCCC
CCTGGCCCCC CTGGCCTTGG TGGAAACTTT GCTGCCCAGA TGGCTGGTGG ATTTGATGAG
AAGGCTGGTG GCGCCCAGAT GGGAGTAATG CAGGGACCAA TGGGCCCCAT GGGACCTCGG
GGACCCCCAG GCCCTGCTGG TGCTCCCGGA CCTCAAGGAT TTCAAGGCAA CCCTGGTGAA
CCTGGTGAAC CCGGTGTTTC TGGTCCCATG GGCCCCCGTG GTCCTCCTGG CCCCCCTGGG
AAACCTGGTG ATGATGGTGA AGCTGGAAAG CCTGGAAAAT CTGGCGAGAG AGGCCCTCCT
GGCCCTCAGG GTGCTCGTGG TTTCCCAGGA ACCCCAGGCC TTCCTGGTGT CAAAGGTCAC
AGAGGTTACC CAGGTCTAGA TGGTGCTAAG GGAGAAGCTG GTGCTCCAGG TGTGAAGGGT
GAGAGTGGTT CCCCAGGTGA GAACGGTTCT CCAGGCCCAA TGGGTCCCCG CGGCTTGCCT
GGTGAGAGAG GACGGACTGG CCCTGCCGGC GCTGCAGGTG CTCGAGGCAA TGACGGTCAG
CCAGGCCCTG CAGGGCCTCC GGGTCCTGTG GGTCCTGCTG GCGGTCCTGG CTTTCCTGGT
GCTCCTGGTG CCAAGGGTGA AGCTGGCCCC ACCGGTGCTC GAGGTCCCGA AGGCGCCCAG
GGTCCTCGCG GTGAACCGGG TACTCCTGGG TCCCCTGGGC CAGCTGGTGC TGCTGGCAAC
CCTGGAACTG ATGGAATCCC TGGAGCCAAG GGATCTGCTG GTGCCCCTGG CATTGCTGGT
GCTCCCGGCT TCCCTGGACC CCGTGGTCCA CCCGGCCCTC AAGGTGCAAC TGGTCCTCTG
GGCCCAAAAG GTCAAACGGG TGAGCCTGGT ATTGCTGGCT TCAAAGGCGA ACAAGGCCCC
AAGGGAGAAC CGGGCCCTGC TGGTCCCCAA GGAGCCCCTG GTCCTGCTGG TGAAGAAGGG
AAAAGAGGTG CCCGTGGAGA ACCTGGTGGT GCTGGGCCCG CCGGTCCCCC TGGAGAAAGA
GGCGCTCCTG GAAACCGTGG TTTCCCAGGT CAGGATGGTC TGGCAGGTCC CAAGGGAGCC
CCTGGAGAGC GAGGACCCAG TGGCCTCGCT GGTCCCAAAG GCGCCAACGG TGACCCTGGC
CGTCCCGGAG AGCCTGGCCT TCCTGGAGCC CGGGGTCTCA CTGGTCGCCC TGGTGATGCT
GGTCCTCAAG GCAAAGTTGG TCCTTCCGGA GCCCCTGGTG AAGACGGTCG CCCTGGACCC
CCAGGTCCTC AGGGGGCTCG TGGGCAGCCT GGTGTCATGG GTTTCCCTGG CCCCAAAGGT
GCCAATGGTG AGCCTGGCAA AGCTGGTGAG AAAGGACTGC CTGGTGCTCC TGGTCTGAGA
GGTCTTCCTG GCAAAGATGG TGAGACAGGT GCTGCAGGCC CCCCTGGACC CGCTGGACCA
GCTGGTGAAC GAGGCGAGCA GGGTGCTCCT GGGCCATCTG GGTTCCAGGG ACTTCCTGGC
CCTCCCGGCC CCCCAGGTGA AGGTGGAAAA CCAGGTGACC AGGGTGTTCC TGGTGAAGCT
GGAGCTCCCG GCCTCGTGGG TCCCAGGGGT GAACGAGGTT TCCCAGGAGA ACGTGGCTCC
CCCGGTTCCC AGGGCCTCCA GGGGGCCCGC GGTCTCCCCG GCACTCCTGG CACTGATGGT
CCCAAAGGCG CAGCTGGCCC AGCTGGCCCC CCTGGGGCTC AGGGCCCTCC AGGTCTGCAG
GGCATGCCCG GTGAGAGGGG AGCAGCTGGT ATCGCCGGAC CCAAGGGAGA CAGGGGTGAT
GTTGGTGAGA AAGGCCCCGA GGGCGCCCCC GGGAAGGACG GTGGACGAGG TCTGACTGGC
CCCATTGGCC CCCCTGGCCC AGCCGGAGCC AACGGTGAGA AGGGAGAAGT TGGACCCCCT
GGTCCTGCTG GAACTGCTGG TGCTCGAGGT GCCCCGGGTG AACGTGGAGA GACAGGCCCC
CCTGGACCTG CTGGATTCGC CGGTCCCCCT GGTGCTGATG GCCAGCCTGG TGCCAAAGGT
GAACAAGGAG AAGCCGGCCA GAAAGGTGAC GCTGGTGCCC CCGGTCCTCA GGGCCCCTCT
GGAGCTCCTG GGCCTCAGGG TCCTACTGGT GTGACCGGTC CTAAAGGAGC CCGAGGTGCT
CAAGGGCCCC CGGGAGCCAC CGGATTCCCC GGAGCAGCTG GCCGCGTCGG ACCCCCAGGC
TCCAATGGCA ACCCTGGACC CCCTGGTCCT CCTGGTCCTT CTGGAAAAGA TGGTCCTAAA
GGTGCTCGAG GCGACAGTGG GCCCCCTGGC CGAGCTGGTG ACCCTGGCCT CCAAGGTCCT
GCTGGACCCC CTGGCGAGAA GGGAGAGCCT GGAGATGATG GTCCTTCTGG TCCCGATGGT
CCTCCAGGTC CTCAGGGTCT GGCTGGTCAG AGGGGCATCG TTGGTCTGCC TGGACAGCGT
GGTGAGCGAG GATTCCCCGG CCTCCCCGGC CCCTCGGGTG AGCCTGGCAA GCAGGGAGCC
CCTGGAGCAT CTGGAGACCG AGGTCCCCCT GGCCCTGTGG GTCCTCCTGG CCTGACTGGT
CCTGCTGGTG AGCCTGGACG TGAGGGAAGC CCCGGTGCTG ACGGCCCCCC TGGCAGAGAT
GGCGCAGCTG GAGTCAAAGG TGACCGTGGA GAGACCGGTG CCGTGGGTGC TCCTGGAGCC
CCTGGGCCCC CTGGCTCTCC TGGCCCCGCT GGCCCAATTG GCAAGCAGGG AGACCGAGGA
GAAGCTGGCG CACAAGGTCC CATGGGACCT GCAGGACCTG CTGGAGCCCG GGGAATGCCA
GGCCCTCAAG GCCCCCGAGG TGACAAAGGA GAAACTGGAG AGGCTGGCGA GAGGGGACTG
AAGGGACACC GTGGCTTCAC TGGTCTGCAG GGTCTGCCCG GCCCCCCTGG TCCTTCTGGA
GACCAGGGTG CTTCCGGTCC TGCAGGTCCT TCCGGCCCCA GAGGTCCTCC TGGTCCCGTC
GGTCCCTCTG GCAAAGATGG TGCTAACGGA ATCCCTGGCC CCATTGGACC TCCTGGGCCC
CGTGGACGTT CCGGCGAGAC TGGCCCTGCT GGTCCTCCTG GAAACCCTGG ACCCCCTGGC
CCTCCTGGCC CCCCCGGCCC TGGCATCGAC ATGTCCGCCT TTGCTGGCCT TGGCCAGAGA
GAGAAAGGCC CCGATCCTCT GCAGTACATG CGGGCTGACG AGGCAGCCGG CAACCTGAGA
CAGCATGATG CCGAGGTGGA CGCCACACTC AAGTCCCTCA ACAACCAGAT CGAGAGCCTC
CGTAGCCCCG AGGGCTCCCG CAAGAACCCA GCTCGCACCT GCCGAGACCT GAAACTCTGC
CACCCTGAGT GGAAGAGCGG AGACTACTGG ATCGACCCCA ACCAGGGCTG CACCCTGGAT
GCCATGAAGG TTTTCTGCAA CATGGAGACT GGCGAGACCT GCGTCTACCC CAACCCGGCC
AGCGTCCCCA AGAAGAACTG GTGGAGCAGC AAGAGCAAGG ACAAGAAACA CATCTGGTTT
GGAGAAACCA TCAACGGTGG CTTCCACTTC AGCTATGGAG ATGACAACCT GGCTCCCAAC
ACCGCCAACG TCCAGATGAC CTTCCTGCGC CTGCTGTCCA CCGAGGGCTC TCAGAACATC
ACCTACCACT GCAAGAACAG CATTGCCTAC CTGGACGAAG CTGCTGGCAA CCTCAAGAAG
GCTCTGCTCA TCCAGGGCTC CAACGACGTG GAGATCCGGG CTGAGGGCAA CAGCAGGTTC
ACATATACCG TTCTGAAGGA TGGCTGCACG AAACACACCG GTAAGTGGGG CAAGACTATG
ATCGAGTACC GGTCACAGAA GACCTCCCGT CTGCCCATCA TTGACATTGC ACCCATGGAC
ATAGGAGGGC CCGAGCAGGA ATTCGGTGTG GACATAGGGC CTGTCTGCTT CTTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.