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Home » Species Summary » Drosophila melanogaster » His2Av cDNA ORF clone
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His2Av cDNA ORF clone, Drosophila melanogaster (fruit fly)

Gene Symbol His2Av
Entrez Gene ID 43229
Full Name Histone H2A variant
Synonyms 5499, CG5499, DmelCG5499, H2A, H2A.F/Z, H2A.V, H2A.X, H2A.Z, H2AV, H2AV_DROM, H2AX, H2AZ, H2Av, H2AvD, H2a.V, H2av, H2avD, HIS, HIS2AV, HIS2AVD, His, His2, His2A, His2AV, His2AvD, HisH2Av, Hist, Hist2av, gamma-H2Av, gamma-HIS2AV, gamma-His2Av, gammaH2Av, h2AvD, his, his2Av, l(3)05146
General protein information
Preferred Names
CG5499 gene product from transcript CG5499-RB
Names
CG5499-PA
CG5499-PB
H2A variant
His2Av-PA
His2Av-PB
Histone-2A
Histone2Av
histone
histone 2A variant
histone H2A variant
histone H2Av
histone variant H2Av
histone2A
histone2AvD
Gene Type protein-coding
Organism Drosophila melanogaster (fruit fly)
Genome

3R

97D3-97D3

Summary lac of sum
Disorder MIM:

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_001276068 NP_001262997 histone H2A variant, isoform B
NM_079795 NP_524519 histone H2A variant, isoform A



Homo sapiens (human) H2AFV NP_036544.1
Pan troglodytes (chimpanzee) H2AFV XP_510606.3
Macaca mulatta (Rhesus monkey) LOC701962 XP_001097247.1
Bos taurus (cattle) H2AFV NP_001033286.1
Mus musculus (house mouse) H2afv NP_084214.1
Rattus norvegicus (Norway rat) LOC685909 NP_001099489.1
Danio rerio (zebrafish) h2afva NP_705930.1
Drosophila melanogaster (fruit fly) His2Av NP_524519.1
Caenorhabditis elegans htz-1 NP_500569.1
Arabidopsis thaliana (thale cress) HTA11 NP_191019.1
Arabidopsis thaliana (thale cress) HTA8 NP_850299.1
Xenopus (Silurana) tropicalis (western clawed frog) h2afv NP_001016178.1

Component

ID Name Evidence
GO:0000786 nucleosome IEA
GO:0000786 nucleosome NAS
GO:0005634 nucleus IDA
GO:0005694 chromosome IDA
GO:0005700 polytene chromosome IDA
GO:0005811 lipid particle IDA

Function

ID Name Evidence
GO:0003677 DNA binding IEA
GO:0003677 DNA binding NAS

Process

ID Name Evidence
GO:0006333 chromatin assembly or disassembly NAS
GO:0006334 nucleosome assembly IEA
GO:0006974 response to DNA damage stimulus IMP

The following His2Av gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the His2Av cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OFa06243 NM_001276068 Drosophila melanogaster histone H2A variant (His2Av), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $149.00
OFa06243 NM_079795 Drosophila melanogaster histone H2A variant (His2Av), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $149.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OFa06243
Accession Version NM_001276068.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 426bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 05-AUG-2014
Organism Drosophila melanogaster (fruit fly)
Product histone H2A variant, isoform B
Comment REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777).

1
61
121
181
241
301
361
421
ATGGCTGGCG GTAAAGCAGG CAAGGATTCG GGCAAGGCCA AGGCGAAGGC GGTATCGCGT 
TCCGCGCGCG CGGGTCTTCA GTTCCCCGTG GGTCGCATCC ATCGTCATCT CAAGAGCCGC
ACTACGTCAC ATGGACGCGT CGGAGCCACT GCAGCCGTGT ACTCCGCTGC CATATTGGAA
TACCTGACCG CCGAGGTCCT GGAGTTGGCA GGCAACGCAT CGAAGGACTT GAAAGTGAAA
CGTATCACTC CTCGCCACTT ACAGCTCGCC ATTCGCGGAG ACGAGGAGCT GGACAGCCTG
ATCAAGGCAA CCATCGCTGG TGGCGGTGTC ATTCCGCACA TACACAAGTC GCTGATCGGC
AAAAAGGAGG AAACGGTGCA GGATCCGCAG CGGAAGGGCA ACGTCATTCT GTCGCAGGCC
TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001262997.1
CDS165..590
Misc Feature(1)171..560(+)
Misc Feature(2)186..530(+)
Misc Feature(3)186..200(+)
Misc Feature(4)258..407(+)
Misc Feature(5)285..299(+)
Misc Feature(6)327..365(+)
Misc Feature(7)528..530(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_001276068
Organism Drosophila melanogaster (fruit fly)
Definition Drosophila melanogaster histone H2A variant (His2Av), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276068

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCTGGCG GTAAAGCAGG CAAGGATTCG GGCAAGGCCA AGGCGAAGGC GGTATCGCGT 
TCCGCGCGCG CGGGTCTTCA GTTCCCCGTG GGTCGCATCC ATCGTCATCT CAAGAGCCGC
ACTACGTCAC ATGGACGCGT CGGAGCCACT GCAGCCGTGT ACTCCGCTGC CATATTGGAA
TACCTGACCG CCGAGGTCCT GGAGTTGGCA GGCAACGCAT CGAAGGACTT GAAAGTGAAA
CGTATCACTC CTCGCCACTT ACAGCTCGCC ATTCGCGGAG ACGAGGAGCT GGACAGCCTG
ATCAAGGCAA CCATCGCTGG TGGCGGTGTC ATTCCGCACA TACACAAGTC GCTGATCGGC
AAAAAGGAGG AAACGGTGCA GGATCCGCAG CGGAAGGGCA ACGTCATTCT GTCGCAGGCC
TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa06243
Accession Version NM_079795.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 426bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 05-AUG-2014
Organism Drosophila melanogaster (fruit fly)
Product histone H2A variant, isoform A
Comment REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced gi:24650418.

1
61
121
181
241
301
361
421
ATGGCTGGCG GTAAAGCAGG CAAGGATTCG GGCAAGGCCA AGGCGAAGGC GGTATCGCGT 
TCCGCGCGCG CGGGTCTTCA GTTCCCCGTG GGTCGCATCC ATCGTCATCT CAAGAGCCGC
ACTACGTCAC ATGGACGCGT CGGAGCCACT GCAGCCGTGT ACTCCGCTGC CATATTGGAA
TACCTGACCG CCGAGGTCCT GGAGTTGGCA GGCAACGCAT CGAAGGACTT GAAAGTGAAA
CGTATCACTC CTCGCCACTT ACAGCTCGCC ATTCGCGGAG ACGAGGAGCT GGACAGCCTG
ATCAAGGCAA CCATCGCTGG TGGCGGTGTC ATTCCGCACA TACACAAGTC GCTGATCGGC
AAAAAGGAGG AAACGGTGCA GGATCCGCAG CGGAAGGGCA ACGTCATTCT GTCGCAGGCC
TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_524519.1
CDS165..590
Misc Feature(1)171..560(+)
Misc Feature(2)186..530(+)
Misc Feature(3)186..200(+)
Misc Feature(4)258..407(+)
Misc Feature(5)285..299(+)
Misc Feature(6)327..365(+)
Misc Feature(7)528..530(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_079795
Organism Drosophila melanogaster (fruit fly)
Definition Drosophila melanogaster histone H2A variant (His2Av), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_079795

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCTGGCG GTAAAGCAGG CAAGGATTCG GGCAAGGCCA AGGCGAAGGC GGTATCGCGT 
TCCGCGCGCG CGGGTCTTCA GTTCCCCGTG GGTCGCATCC ATCGTCATCT CAAGAGCCGC
ACTACGTCAC ATGGACGCGT CGGAGCCACT GCAGCCGTGT ACTCCGCTGC CATATTGGAA
TACCTGACCG CCGAGGTCCT GGAGTTGGCA GGCAACGCAT CGAAGGACTT GAAAGTGAAA
CGTATCACTC CTCGCCACTT ACAGCTCGCC ATTCGCGGAG ACGAGGAGCT GGACAGCCTG
ATCAAGGCAA CCATCGCTGG TGGCGGTGTC ATTCCGCACA TACACAAGTC GCTGATCGGC
AAAAAGGAGG AAACGGTGCA GGATCCGCAG CGGAAGGGCA ACGTCATTCT GTCGCAGGCC
TACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Sequence finishing and mapping of Drosophila melanogaster heterochromatin
Science 316 (5831), 1625-1628 (2007)
Hoskins,R.A., Carlson,J.W., Kennedy,C., Acevedo,D., Evans-Holm,M., Frise,E., Wan,K.H., Park,S., Mendez-Lago,M., Rossi,F., Villasante,A., Dimitri,P., Karpen,G.H. and Celniker,S.E.


book

The Release 5.1 annotation of Drosophila melanogaster heterochromatin
Science 316 (5831), 1586-1591 (2007)
Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H.


book

Combined evidence annotation of transposable elements in genome sequences
PLoS Comput. Biol. 1 (2), 166-175 (2005)
Quesneville,H., Bergman,C.M., Andrieu,O., Autard,D., Nouaud,D., Ashburner,M. and Anxolabehere,D.


book

Heterochromatic sequences in a Drosophila whole-genome shotgun assembly
Genome Biol. 3 (12), RESEARCH0085 (2002)
Hoskins,R.A., Smith,C.D., Carlson,J.W., Carvalho,A.B., Halpern,A., Kaminker,J.S., Kennedy,C., Mungall,C.J., Sullivan,B.A., Sutton,G.G., Yasuhara,J.C., Wakimoto,B.T., Myers,E.W., Celniker,S.E., Rubin,G.M. and Karpen,G.H.


book

The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective
Genome Biol. 3 (12), RESEARCH0084 (2002)
Kaminker,J.S., Bergman,C.M., Kronmiller,B., Carlson,J., Svirskas,R., Patel,S., Frise,E., Wheeler,D.A., Lewis,S.E., Rubin,G.M., Ashburner,M. and Celniker,S.E.


book

Annotation of the Drosophila melanogaster euchromatic genome: a systematic review
Genome Biol. 3 (12), RESEARCH0083 (2002)
Misra,S., Crosby,M.A., Mungall,C.J., Matthews,B.B., Campbell,K.S., Hradecky,P., Huang,Y., Kaminker,J.S., Millburn,G.H., Prochnik,S.E., Smith,C.D., Tupy,J.L., Whitfied,E.J., Bayraktaroglu,L., Berman,B.P., Bettencourt,B.R., Celniker,S.E., de Grey,A.D., Drysdale,R.A., Harris,N.L., Richter,J., Russo,S., Schroeder,A.J., Shu,S.Q., Stapleton,M., Yamada,C., Ashburner,M., Gelbart,W.M., Rubin,G.M. and Lewis,S.E.


book

Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster euchromatic genome sequence
Genome Biol. 3 (12), RESEARCH0079 (2002)
Celniker,S.E., Wheeler,D.A., Kronmiller,B., Carlson,J.W., Halpern,A., Patel,S., Adams,M., Champe,M., Dugan,S.P., Frise,E., Hodgson,A., George,R.A., Hoskins,R.A., Laverty,T., Muzny,D.M., Nelson,C.R., Pacleb,J.M., Park,S., Pfeiffer,B.D., Richards,S., Sodergren,E.J., Svirskas,R., Tabor,P.E., Wan,K., Stapleton,M., Sutton,G.G., Venter,C., Weinstock,G., Scherer,S.E., Myers,E.W., Gibbs,R.A. and Rubin,G.M.


book

The genome sequence of Drosophila melanogaster
Science 287 (5461), 2185-2195 (2000)
Adams,M.D., Celniker,S.E., Holt,R.A., Evans,C.A., Gocayne,J.D., Amanatides,P.G., Scherer,S.E., Li,P.W., Hoskins,R.A., Galle,R.F., George,R.A., Lewis,S.E., Richards,S., Ashburner,M., Henderson,S.N., Sutton,G.G., Wortman,J.R., Yandell,M.D., Zhang,Q., Chen,L.X., Brandon,R.C., Rogers,Y.H., Blazej,R.G., Champe,M., Pfeiffer,B.D., Wan,K.H., Doyle,C., Baxter,E.G., Helt,G., Nelson,C.R., Gabor,G.L., Abril,J.F., Agbayani,A., An,H.J., Andrews-Pfannkoch,C., Baldwin,D., Ballew,R.M., Basu,A., Baxendale,J., Bayraktaroglu,L., Beasley,E.M., Beeson,K.Y., Benos,P.V., Berman,B.P., Bhandari,D., Bolshakov,S., Borkova,D., Botchan,M.R., Bouck,J., Brokstein,P., Brottier,P., Burtis,K.C., Busam,D.A., Butler,H., Cadieu,E., Center,A., Chandra,I., Cherry,J.M., Cawley,S., Dahlke,C., Davenport,L.B., Davies,P., de Pablos,B., Delcher,A., Deng,Z., Mays,A.D., Dew,I., Dietz,S.M., Dodson,K., Doup,L.E., Downes,M., Dugan-Rocha,S., Dunkov,B.C., Dunn,P., Durbin,K.J., Evangelista,C.C., Ferraz,C., Ferriera,S., Fleischmann,W., Fosler,C., Gabrielian,A.E., Garg,N.S., Gelbart,W.M., Glasser,K., Glodek,A., Gong,F., Gorrell,J.H., Gu,Z., Guan,P., Harris,M., Harris,N.L., Harvey,D., Heiman,T.J., Hernandez,J.R., Houck,J., Hostin,D., Houston,K.A., Howland,T.J., Wei,M.H., Ibegwam,C., Jalali,M., Kalush,F., Karpen,G.H., Ke,Z., Kennison,J.A., Ketchum,K.A., Kimmel,B.E., Kodira,C.D., Kraft,C., Kravitz,S., Kulp,D., Lai,Z., Lasko,P., Lei,Y., Levitsky,A.A., Li,J., Li,Z., Liang,Y., Lin,X., Liu,X., Mattei,B., McIntosh,T.C., McLeod,M.P., McPherson,D., Merkulov,G., Milshina,N.V., Mobarry,C., Morris,J., Moshrefi,A., Mount,S.M., Moy,M., Murphy,B., Murphy,L., Muzny,D.M., Nelson,D.L., Nelson,D.R., Nelson,K.A., Nixon,K., Nusskern,D.R., Pacleb,J.M., Palazzolo,M., Pittman,G.S., Pan,S., Pollard,J., Puri,V., Reese,M.G., Reinert,K., Remington,K., Saunders,R.D., Scheeler,F., Shen,H., Shue,B.C., Siden-Kiamos,I., Simpson,M., Skupski,M.P., Smith,T., Spier,E., Spradling,A.C., Stapleton,M., Strong,R., Sun,E., Svirskas,R., Tector,C., Turner,R., Venter,E., Wang,A.H., Wang,X., Wang,Z.Y., Wassarman,D.A., Weinstock,G.M., Weissenbach,J., Williams,S.M., WoodageT, Worley,K.C., Wu,D., Yang,S., Yao,Q.A., Ye,J., Yeh,R.F., Zaveri,J.S., Zhan,M., Zhang,G., Zhao,Q., Zheng,L., Zheng,X.H., Zhong,F.N., Zhong,W., Zhou,X., Zhu,S., Zhu,X., Smith,H.O., Gibbs,R.A., Myers,E.W., Rubin,G.M. and Venter,J.C.


book

Drosophila melanogaster release 4 sequence
Unpublished
Celniker,S., Carlson,J., Wan,K., Pfeiffer,B., Frise,E., George,R., Hoskins,R., Stapleton,M., Pacleb,J., Park,S., Svirskas,R., Smith,E., Yu,C. and Rubin,G.


book

Direct Submission
Submitted (24-JUL-2014) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA


book

Direct Submission
Submitted (27-MAR-2014) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA


book

Direct Submission
Submitted (06-DEC-2012) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA


book

Direct Submission
Submitted (05-APR-2012) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA


book

Direct Submission
Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One #Cyclotron RoadOne Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA
Celniker,S., Carlson,J., Kennedy,C., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Karpen,G.


book

Direct Submission
Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA
Celniker,S., Carlson,J., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Rubin,G.


book

Direct Submission
Submitted (01-AUG-2006) Drosophila Heterochromatin Genome Project, Ernest Orlando Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 64-121, Berkeley, CA 94720, USA
Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H.


book

Direct Submission
Submitted (21-MAR-2000) Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA
Adams,M.D., Celniker,S.E., Gibbs,R.A., Rubin,G.M. and Venter,C.J.


 
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