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PIGK cDNA ORF clone, Homo sapiens (human)

Gene Symbol PIGK
Entrez Gene ID 10026
Full Name phosphatidylinositol glycan anchor biosynthesis, class K
Synonyms GPI8
General protein information
Preferred Names
GPI-anchor transamidase
Names
GPI-anchor transamidase
PIG-K
GPI8 homolog
GPI transamidase subunit
phosphatidylinositol glycan, class K
phosphatidylinositol-glycan biosynthesis class K protein
NP_005473.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p31.1

Summary This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

605087


mRNA and Protein(s)

mRNA Protein Name
NM_005482 NP_005473 GPI-anchor transamidase precursor



Homo sapiens (human) PIGK NP_005473.1
Pan troglodytes (chimpanzee) PIGK XP_001168438.1
Macaca mulatta (Rhesus monkey) PIGK XP_001102474.1
Canis lupus familiaris (dog) PIGK XP_853775.1
Bos taurus (cattle) PIGK NP_001071348.1
Mus musculus (house mouse) Pigk NP_079938.1
Rattus norvegicus (Norway rat) Pigk NP_001011953.1
Gallus gallus (chicken) PIGK NP_001026449.1
Danio rerio (zebrafish) pigk NP_001002149.1
Drosophila melanogaster (fruit fly) CG4406 NP_569968.2
Caenorhabditis elegans hpo-4 NP_502076.2
Arabidopsis thaliana (thale cress) AT1G08750 NP_849616.1
Xenopus (Silurana) tropicalis (western clawed frog) pigk NP_001072709.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PIGK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIGK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu04946
NM_005482 Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class K (PIGK), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu04946
Clone ID Related Accession (Same CDS sequence) NM_005482
Accession Version NM_005482.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product GPI-anchor transamidase precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA263434.1, AF022913.1 and AL035409.15. On Jul 6, 2006 this sequence version replaced gi:23199982. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF022913.1, Y07596.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCCGTCA CCGACAGCCT CAGCCGGGCT GCGACTGTCT TGGCAACTGT GTTGCTCTTG 
TCCTTCGGCA GCGTGGCCGC TAGTCATATC GAGGATCAAG CAGAACAATT CTTTAGAAGT
GGCCATACAA ACAACTGGGC TGTTCTGGTG TGTACATCCC GATTCTGGTT TAATTATCGA
CATGTTGCAA ATACCCTTTC TGTTTATAGA AGTGTCAAGA GGCTAGGTAT TCCTGACAGT
CACATTGTCC TAATGCTTGC AGATGATATG GCCTGTAATC CTAGAAATCC CAAACCAGCT
ACAGTGTTTA GTCACAAGAA TATGGAACTA AATGTGTATG GAGATGATGT GGAAGTGGAT
TATAGAAGTT ATGAGGTAAC TGTGGAGAAT TTTTTACGGG TATTAACTGG GAGGATCCCA
CCTAGTACTC CTCGGTCAAA ACGTCTTCTT TCTGATGACA GAAGCAATAT TCTAATTTAT
ATGACAGGGC ATGGTGGAAA TGGTTTCTTA AAATTTCAAG ATTCTGAAGA AATTACCAAC
ATAGAACTCG CGGATGCTTT TGAACAAATG TGGCAGAAAA GACGCTACAA TGAGCTACTG
TTTATTATTG ATACTTGCCA AGGAGCATCC ATGTATGAAC GATTTTATTC TCCTAACATA
ATGGCTCTAG CTAGTAGTCA AGTGGGAGAA GATTCACTCT CGCATCAACC TGATCCTGCA
ATTGGAGTCC ATCTTATGGA TAGATACACA TTTTATGTCT TGGAATTTTT GGAAGAAATT
AACCCAGCTA GCCAAACTAA TATGAATGAC CTTTTTCAGG TATGTCCCAA AAGTCTGTGT
GTGTCTACTC CTGGACATCG CACTGATCTT TTTCAGAGGG ATCCTAAAAA TGTACTGATA
ACTGATTTCT TTGGAAGTGT ACGGAAAGTG GAAATTACAA CAGAGACTAT TAAATTGCAA
CAGGATTCAG AAATCATGGA AAGCAGCTAT AAGGAAGACC AGATGGATGA GAAACTAATG
GAACCTCTGA AATATGCTGA ACAACTTCCT GTAGCTCAGA TAATACACCA GAAACCGAAG
CTGAAAGACT GGCATCCTCC TGGGGGCTTT ATTCTGGGAT TATGGGCACT TATTATCATG
GTTTTCTTCA AAACTTATGG AATTAAGCAT ATGAAGTTCA TTTTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005473.1
CDS46..1233
Misc Feature(1)169..1038(+)
Misc Feature(2)976..1008(+)
Misc Feature(3)1147..1209(+)
Exon (1)1..138
Gene:PIGK
Gene Synonym:
Exon (2)139..192
Gene:PIGK
Gene Synonym:
Exon (3)193..284
Gene:PIGK
Gene Synonym:
Exon (4)285..420
Gene:PIGK
Gene Synonym:
Exon (5)421..532
Gene:PIGK
Gene Synonym:
Exon (6)533..629
Gene:PIGK
Gene Synonym:
Exon (7)630..747
Gene:PIGK
Gene Synonym:
Exon (8)748..858
Gene:PIGK
Gene Synonym:
Exon (9)859..1031
Gene:PIGK
Gene Synonym:
Exon (10)1032..1116
Gene:PIGK
Gene Synonym:
Exon (11)1117..4626
Gene:PIGK
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005482
Organism Homo sapiens (human)
Definition Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class K (PIGK), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005482

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCCGTCA CCGACAGCCT CAGCCGGGCT GCGACTGTCT TGGCAACTGT GTTGCTCTTG 
TCCTTCGGCA GCGTGGCCGC TAGTCATATC GAGGATCAAG CAGAACAATT CTTTAGAAGT
GGCCATACAA ACAACTGGGC TGTTCTGGTG TGTACATCCC GATTCTGGTT TAATTATCGA
CATGTTGCAA ATACCCTTTC TGTTTATAGA AGTGTCAAGA GGCTAGGTAT TCCTGACAGT
CACATTGTCC TAATGCTTGC AGATGATATG GCCTGTAATC CTAGAAATCC CAAACCAGCT
ACAGTGTTTA GTCACAAGAA TATGGAACTA AATGTGTATG GAGATGATGT GGAAGTGGAT
TATAGAAGTT ATGAGGTAAC TGTGGAGAAT TTTTTACGGG TATTAACTGG GAGGATCCCA
CCTAGTACTC CTCGGTCAAA ACGTCTTCTT TCTGATGACA GAAGCAATAT TCTAATTTAT
ATGACAGGGC ATGGTGGAAA TGGTTTCTTA AAATTTCAAG ATTCTGAAGA AATTACCAAC
ATAGAACTCG CGGATGCTTT TGAACAAATG TGGCAGAAAA GACGCTACAA TGAGCTACTG
TTTATTATTG ATACTTGCCA AGGAGCATCC ATGTATGAAC GATTTTATTC TCCTAACATA
ATGGCTCTAG CTAGTAGTCA AGTGGGAGAA GATTCACTCT CGCATCAACC TGATCCTGCA
ATTGGAGTCC ATCTTATGGA TAGATACACA TTTTATGTCT TGGAATTTTT GGAAGAAATT
AACCCAGCTA GCCAAACTAA TATGAATGAC CTTTTTCAGG TATGTCCCAA AAGTCTGTGT
GTGTCTACTC CTGGACATCG CACTGATCTT TTTCAGAGGG ATCCTAAAAA TGTACTGATA
ACTGATTTCT TTGGAAGTGT ACGGAAAGTG GAAATTACAA CAGAGACTAT TAAATTGCAA
CAGGATTCAG AAATCATGGA AAGCAGCTAT AAGGAAGACC AGATGGATGA GAAACTAATG
GAACCTCTGA AATATGCTGA ACAACTTCCT GTAGCTCAGA TAATACACCA GAAACCGAAG
CTGAAAGACT GGCATCCTCC TGGGGGCTTT ATTCTGGGAT TATGGGCACT TATTATCATG
GTTTTCTTCA AAACTTATGG AATTAAGCAT ATGAAGTTCA TTTTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A single nucleotide polymorphism in the human PIGK gene associates with low PIGK expression in colorectal cancer patients
Int. J. Oncol. 41 (4), 1405-1410 (2012)
Dasgupta S, Pal P, Mukhopadhyay ND, Fu Y, Ratovitski EA, Moon CS, Hoque MO, Fisher PB and Trink B.


book

hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes
Genomics 89 (3), 307-315 (2007)
Lamesch P, Li N, Milstein S, Fan C, Hao T, Szabo G, Hu Z, Venkatesan K, Bethel G, Martin P, Rogers J, Lawlor S, McLaren S, Dricot A, Borick H, Cusick ME, Vandenhaute J, Dunham I, Hill DE and Vidal M.


book

Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins
Mol. Biol. Cell 14 (5), 1780-1789 (2003)
Hong Y, Ohishi K, Kang JY, Tanaka S, Inoue N, Nishimura J, Maeda Y and Kinoshita T.


book

Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge
J. Biol. Chem. 278 (16), 13959-13967 (2003)
Ohishi K, Nagamune K, Maeda Y and Kinoshita T.


book

Proprotein interaction with the GPI transamidase
J. Cell. Biochem. 88 (5), 1025-1037 (2003)
Chen R, Anderson V, Hiroi Y and Medof ME.


book

Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins
Mol. Biol. Cell 11 (5), 1523-1533 (2000)
Ohishi K, Inoue N, Maeda Y, Takeda J, Riezman H and Kinoshita T.


book

Active site determination of Gpi8p, a caspase-related enzyme required for glycosylphosphatidylinositol anchor addition to proteins
Biochemistry 39 (12), 3461-3471 (2000)
Meyer U, Benghezal M, Imhof I and Conzelmann A.


book

The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase
Proc. Natl. Acad. Sci. U.S.A. 94 (23), 12580-12585 (1997)
Yu J, Nagarajan S, Knez JJ, Udenfriend S, Chen R and Medof ME.


book

Yeast Gpi8p is essential for GPI anchor attachment onto proteins
EMBO J. 15 (23), 6575-6583 (1996)
Benghezal M, Benachour A, Rusconi S, Aebi M and Conzelmann A.


book

Cellular receptor for urokinase plasminogen activator. Carboxyl-terminal processing and membrane anchoring by glycosyl-phosphatidylinositol
J. Biol. Chem. 266 (3), 1926-1933 (1991)
Ploug M, Ronne E, Behrendt N, Jensen AL, Blasi F and Dano K.


 
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