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ORF cDNA Clones and Custom Clones » Species Summary » Homo sapiens » MAGEA10-MAGEA5 cDNA ORF clone
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MAGEA10-MAGEA5 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAGEA10-MAGEA5
Entrez Gene ID 100533997
Full Name MAGEA10-MAGEA5 readthrough
Synonyms CT1.5
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq28

Summary This locus represents naturally occurring read-through transcription between the neighboring MAGEA10 (melanoma antigen family A, 10) and MAGEA5 (melanoma antigen family A, 5) genes on chromosome X. The read-through transcript is predicted to encode the downstream gene product. [provided by RefSeq, Mar 2011]. lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_001204811 NP_001191740 melanoma-associated antigen 5


Homo sapiens (human) MAGEA10-MAGEA5 NP_001191740.1
Homo sapiens (human) MAGEA5 NP_066387.1


Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAGEA10-MAGEA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEA10-MAGEA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu08405 NM_001204811 Homo sapiens MAGEA10-MAGEA5 readthrough (MAGEA10-MAGEA5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu08405
Clone ID Related Accession (Same CDS sequence) NM_001204811 , NM_021049
Accession Version NM_001204811.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 375bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen 5
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116666.2, BX109673.1 and AF274856.3. On Oct 18, 2011 this sequence version replaced gi:325651880. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 4104, 4109 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BX109673.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA2149398 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
ATGTCTCTTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA CACCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGTGCAGGCT GCCACTACTG AGGAGCAGGA GGCTGTGTCC
TCCTCCTCTC CTCTGGTCCC AGGCACCCTG GGGGAGGTGC CTGCTGCTGG GTCACCAGGT
CCTCTCAAGA GTCCTCAGGG AGCCTCCGCC ATCCCCACTG CCATCGATTT CACTCTATGG
AGGCAATCCA TTAAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTCCCCTGAC
CCAGAGTCTG TGTTCCGAGC AGCACTCAGT AAGAAGGTGG CTGACTTGAT TCATTTTCTG
CTCCTCAAGT ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191740.1
CDS382..756
Misc Feature(1)247..249(+)
Misc Feature(2)388..675(+)
Exon (1)1..104
Gene:MAGEA10-MAGEA5
Gene Synonym:
Exon (2)105..229
Gene:MAGEA10-MAGEA5
Gene Synonym:
Exon (3)230..316
Gene:MAGEA10-MAGEA5
Gene Synonym:
Exon (4)317..1873
Gene:MAGEA10-MAGEA5
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204811
Organism Homo sapiens (human)
Definition Homo sapiens MAGEA10-MAGEA5 readthrough (MAGEA10-MAGEA5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204811

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGTCTCTTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA CACCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGTGCAGGCT GCCACTACTG AGGAGCAGGA GGCTGTGTCC
TCCTCCTCTC CTCTGGTCCC AGGCACCCTG GGGGAGGTGC CTGCTGCTGG GTCACCAGGT
CCTCTCAAGA GTCCTCAGGG AGCCTCCGCC ATCCCCACTG CCATCGATTT CACTCTATGG
AGGCAATCCA TTAAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTCCCCTGAC
CCAGAGTCTG TGTTCCGAGC AGCACTCAGT AAGAAGGTGG CTGACTTGAT TCATTTTCTG
CTCCTCAAGT ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Expression of conjoined genes: another mechanism for gene regulation in eukaryotes
PLoS ONE 5 (10), E13284 (2010)
Prakash T, Sharma VK, Adati N, Ozawa R, Kumar N, Nishida Y, Fujikake T, Takeda T and Taylor TD.


book

Structure, chromosomal localization, and expression of 12 genes of the MAGE family
Immunogenetics 40 (5), 360-369 (1994)
De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethe B and Lurquin C.


 
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