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TSPAN32 tetraspanin 32 [Homo sapiens (human)]

Gene Symbol TSPAN32
Entrez Gene ID 10077
Full Name tetraspanin 32
Synonyms ART1, PHEMX, PHMX, TSSC6
General protein information
Preferred Names
tetraspanin-32
Names
tetraspanin-32
tspan-32
protein Phemx
tumor-suppressing STF cDNA 6
pan-hematopoietic expression protein
tumor-suppressing subtransferable candidate 6
tumor-suppressing subchromosomal transferable fragment cDNA 6
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11p15.5

Summary This gene, which is a member of the tetraspanin superfamily, is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. This gene is located among several imprinted genes; however, this gene, as well as the tumor-suppressing subchromosomal transferable fragment 4, escapes imprinting. This gene may play a role in malignancies and diseases that involve this region, and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603853

Disorder Html:





The following TSPAN32 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSPAN32 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu63662 XM_011519809 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu63663 XM_011519810 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu63664 XM_011519811 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu44730 XM_005252719 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu63665 XM_011519812 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu63666 XM_011519813 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu63667 XM_011519814 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu63668 XM_011519815 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63669 XM_011519816 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu63669 XM_011519817 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu63670 XM_011519818 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63671 XM_011519819 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63672 XM_011519820 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63673 XM_011519821 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63674 XM_011519822 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu44731 XM_005252720 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63675 XM_011519823 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63676 XM_011519824 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X19, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63676 XM_011519825 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X20, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63676 XM_011519826 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X21, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63676 XM_011519827 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X22, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu63677 XM_011519828 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X23, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu63678 XM_011519829 PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X24, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu08930 NM_139022 Homo sapiens tetraspanin 32 (TSPAN32), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu63662
Accession Version XM_011519809.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1209bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tetraspanin-32 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCAGCCTA CCTCCTGGGC AGTGAGCTGC GGTCTGAGGC CCCTGCCCAG CTGGAAACCA 
CAGGGAGGGG AAGGGAGGGG AGGAGAGGAG AGGAGAGGAA CCGTCATGGG GCCTTGGAGT
CGAGTCAGGG TTGCCAAATG CCAGATGCTG GTCACCTGCT TCTTTATCTT GCTGCTGGGC
CTCTCTGTGG CCACCATGGT GACTCTTACC TACTTCGGGG CCCACTTTGC TGTCATCCGC
CGAGCGTCCC TGGAGAAGAA CCCGTACCAG GCTGTGCACC AATGGGGGAC TCAGCAGCGA
CTTATCCAAC ATCCAGAGAG CGGGAGCGAG GGCCAGAGCC TGCTGGGGCC ACTCAGGGCC
TTCTCTGCGG GGTTGAGCCT GGTGGGCCTC CTGACTCTGG GAGCCGTGCT GAGCGCTGCA
GCCACCGTGA GGGAGGCCCA GGGCCTCATG GCAGGGGGCT TCCTGTGCTT CTCCCTGGCG
TTCTGCGCAC AGGTGCAGGT GGTGTTCTGG AGACTCCACA GCCCCACCCA GGTGGAGGAC
GCCATGCTGG ACACCTACGA CCTGGTATAT GAGCAGGCGA TGAAAGGTAC GTCCCACGTC
CGGCGGCAGG AGCTGGCGGC CATCCAGGAC GTGTTTCTGT GCTGTGGGAA GAAGTCTCCT
TTCAGCCGTC TGGGGAGCAC AGAGGCTGAC CTGTGTCAGG GAGAGGAGGC GGCGAGAGAG
GACTGCCTTC AGGGCATCCG GAGCTTCCTG AGGACACACC AGCAGGTCGC CTCCAGCCTG
ACCAGCATCG GCCTGGCCCT CACGGTGTCC GCCTTGCTCT TCAGCTCCTT CCTGTGGTTT
GCCATCCGCT GTGGCTGCAG CTTGGACCGC AAGGGCAAAT ACACCCTGAC CCCACGAGCA
TGTGGCCGCC AGCCCCAGGA GCCCAGCCTC TTGAGATGCT CCCAGGGTGG ACCCACACAT
TGTCTCCACT CCGAAGCAGT TGCTATTGGT CCAAGAGGAT GCTCGGGTAG TCTTCGGTGG
CTGCAGGAGA GCGATGCTGC GCCTCTGCCC CTCTCCTGCC ACCTGGCTGC CCACAGAGGT
GAAGACGCCC CTGCTGTCAG CCCTCATGGG ATCCCTGAGG GGAGGGTCCG AGCTGTGAGG
AGGGAAGGGA GTGAAGGCCC AGCCAGAGAG CCAGGCTCCA TTGGGAACAG ATGCAAGGGT
AAGGGGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011518111.1
CDS33..1241
Misc Feature(1)168..878(+)
Misc Feature(2)546..794(+)
Misc Feature(3)549..668(+)
Translation
Position Chain Variation Link
2 2 -, ggg dbSNP:777634526
4 4 a, g dbSNP:371953629
5 5 c, t dbSNP:757112917
6 6 a, c, g dbSNP:780797896
7 7 a, t dbSNP:769288063
10 10 c, t dbSNP:779543727
30 30 c, t dbSNP:748595793
31 31 a, g dbSNP:772487085
34 34 g, t dbSNP:773810149
40 40 c, t dbSNP:760927991
49 49 g, t dbSNP:375443053
62 62 c, g, t dbSNP:78465685
63 63 a, g dbSNP:564849421
70 70 a, g dbSNP:765354088
78 78 a, c dbSNP:372594309
82 82 a, g dbSNP:762904620
95 95 g, t dbSNP:763904934
107 107 a, g dbSNP:375516709
111 111 -, gagga dbSNP:746834021
112 112 -, gagga dbSNP:770612134
131 131 a, g dbSNP:756986960
133 133 a, c dbSNP:781030842
134 134 c, t dbSNP:750010162
135 135 a, g dbSNP:532243385
144 144 a, c dbSNP:149680436
147 147 c, t dbSNP:748716777
153 153 c, t dbSNP:145517785
154 154 a, g dbSNP:148861275
154 154 -, g dbSNP:776304562
162 162 g, t dbSNP:778131898
173 173 a, c dbSNP:747621957
176 176 c, g dbSNP:142403358
181 181 a, c, t dbSNP:777152824
182 182 a, g dbSNP:770167215
195 195 g, t dbSNP:775892076
203 203 a, g dbSNP:762917262
204 204 a, c dbSNP:200210112
211 211 a, g dbSNP:758018002
218 218 g, t dbSNP:543107439
220 220 c, t dbSNP:746432153
225 225 a, t dbSNP:756540607
231 231 g, t dbSNP:780470109
235 235 c, t dbSNP:749412499
242 242 c, t dbSNP:768944967
248 248 c, t dbSNP:146480945
249 249 -, g dbSNP:750786852
253 253 c, t dbSNP:369696526
260 260 c, g, t dbSNP:576767301
268 268 c, t dbSNP:540982577
270 270 a, c, t dbSNP:140709712
271 271 a, g dbSNP:201106606
273 273 a, c, t dbSNP:559404880
274 274 a, g, t dbSNP:371441475
277 277 c, t dbSNP:529974468
278 278 a, g dbSNP:368347803
282 282 c, g dbSNP:762542013
295 295 c, t dbSNP:377338457
299 299 c, t dbSNP:750896014
300 300 a, c dbSNP:756704119
303 303 c, g dbSNP:145905096
304 304 a, c dbSNP:770456957
311 311 a, c dbSNP:755347022
313 313 a, c dbSNP:181067559
314 314 a, g dbSNP:531066922
318 318 a, g dbSNP:748434290
358 358 g, t dbSNP:371482652
371 371 c, t dbSNP:1501467
391 391 c, g dbSNP:774188366
399 399 a, g dbSNP:761371873
401 401 a, g dbSNP:201530910
403 403 a, g dbSNP:777126996
411 411 c, t dbSNP:759972432
412 412 c, t dbSNP:376702018
413 413 c, g dbSNP:765534620
421 421 c, t dbSNP:753089277
424 424 c, t dbSNP:138170136
428 428 g, t dbSNP:555848083
431 431 a, g dbSNP:371071821
432 432 a, g dbSNP:200631781
433 433 c, g dbSNP:751841969
438 438 a, g dbSNP:2234296
446 446 a, c, t dbSNP:199650987
447 447 a, c, g dbSNP:201634419
450 450 g, t dbSNP:749060868
459 459 a, g dbSNP:545681469
464 464 a, g dbSNP:774141716
465 465 a, g dbSNP:747759649
468 468 g, t dbSNP:200624662
476 476 c, t dbSNP:772617887
479 479 c, t dbSNP:760045340
481 481 c, t dbSNP:61744929
485 485 a, g dbSNP:776021550
488 488 a, g dbSNP:763294199
495 495 a, c dbSNP:768954809
506 506 c, t dbSNP:774603549
507 507 a, c dbSNP:372246596
511 511 c, t dbSNP:767604508
512 512 a, g dbSNP:570557887
518 518 a, c, g, t dbSNP:11554947
519 519 a, g dbSNP:770634605
525 525 g, t dbSNP:753770845
528 528 c, t dbSNP:754985828
539 539 a, c dbSNP:778727393
542 542 a, g dbSNP:752399702
543 543 a, g dbSNP:758260127
558 558 a, g dbSNP:376562078
567 567 c, g dbSNP:775228702
569 569 a, g dbSNP:367966944
572 572 c, t dbSNP:2234300
573 573 a, g dbSNP:2234301
585 585 -, acctacg dbSNP:779134770
586 586 c, g dbSNP:757085417
589 589 a, g dbSNP:767136719
590 590 c, t dbSNP:147630481
594 594 a, c dbSNP:755576262
596 596 a, g dbSNP:779628656
605 605 a, g dbSNP:748555005
610 610 c, t dbSNP:758973942
611 611 a, g dbSNP:778462230
619 619 g, t dbSNP:112455917
622 622 c, t dbSNP:554395460
629 629 c, t dbSNP:368872715
633 633 c, t dbSNP:374473348
634 634 a, g dbSNP:138129469
637 637 a, g dbSNP:2234302
649 649 c, t dbSNP:561781505
650 650 a, g dbSNP:764063848
662 662 c, t dbSNP:541981635
663 663 a, g dbSNP:759043414
666 666 c, t dbSNP:772911765
669 669 c, t dbSNP:771804666
674 674 c, t dbSNP:773022730
683 683 a, g dbSNP:760426076
690 690 a, c dbSNP:147219779
699 699 c, t dbSNP:148601311
700 700 a, g dbSNP:142056890
707 707 a, g dbSNP:764840660
715 715 a, t dbSNP:752051464
716 716 a, g dbSNP:757864779
717 717 -, gct dbSNP:752515915
728 728 c, g, t dbSNP:781658568
731 731 a, g dbSNP:756488352
742 742 a, c, t dbSNP:780310923
743 743 a, g dbSNP:768658357
745 745 c, t dbSNP:778979541
746 746 a, g dbSNP:200478299
756 756 g, t dbSNP:771679154
757 757 a, g dbSNP:763277110
759 759 c, t dbSNP:777055649
765 765 a, g dbSNP:760046572
771 771 a, c, t dbSNP:376763237
772 772 a, g dbSNP:766761476
773 773 a, g dbSNP:186572696
775 775 a, g dbSNP:764203991
797 797 g, t dbSNP:369033190
798 798 a, g dbSNP:761799293
800 800 c, t dbSNP:150731413
801 801 a, g dbSNP:371671456
802 802 c, t dbSNP:756144294
815 815 -, cag dbSNP:774995039
822 822 a, g dbSNP:779955859
823 823 a, g dbSNP:753662640
828 828 g, t dbSNP:201400387
831 831 c, g, t dbSNP:778426094
835 835 c, t dbSNP:771421230
836 836 a, g dbSNP:777282787
838 838 -, gtcc dbSNP:772632958
842 842 c, t dbSNP:553461987
843 843 a, g dbSNP:778304554
845 845 c, g dbSNP:747184229
851 851 c, g dbSNP:200678268
853 853 g, t dbSNP:781315038
857 857 c, t dbSNP:745944280
866 866 a, g dbSNP:769833899
877 877 c, t dbSNP:374327578
879 879 a, c, t dbSNP:200026082
880 880 a, g dbSNP:774163392
887 887 c, t dbSNP:369812524
889 889 a, g dbSNP:143112175
891 891 a, g dbSNP:561690224
900 900 c, t dbSNP:767178490
901 901 a, c, g, t dbSNP:750103889
916 916 a, c dbSNP:753206297
924 924 c, g, t dbSNP:377055075
927 927 c, t dbSNP:751947184
928 928 a, g dbSNP:371452736
931 931 c, t dbSNP:756317638
939 939 c, t dbSNP:375291522
940 940 a, g dbSNP:756322762
942 942 c, g dbSNP:780299105
947 947 c, g, t dbSNP:749352222
970 970 g, t dbSNP:3180160
976 976 a, t dbSNP:139081815
981 981 a, g dbSNP:778820239
984 984 -, c dbSNP:773635676
988 988 c, t dbSNP:144063439
994 994 a, g, t dbSNP:146837754
1004 1004 c, t dbSNP:140677453
1005 1005 a, g dbSNP:770684289
1012 1012 a, t dbSNP:776483823
1017 1017 a, g dbSNP:759111860
1020 1020 a, g dbSNP:764842683
1021 1021 a, g dbSNP:774943231
1023 1023 c, t dbSNP:762250888
1026 1026 a, c, g dbSNP:144460879
1027 1027 c, g dbSNP:367851224
1032 1032 c, t dbSNP:34745234
1036 1036 c, t dbSNP:149230396
1037 1037 a, c, g dbSNP:371556972
1041 1041 a, g dbSNP:752637049
1043 1043 c, t dbSNP:758233212
1044 1044 a, c dbSNP:777814788
1047 1047 c, t dbSNP:143225348
1048 1048 a, c, g dbSNP:375834458
1057 1057 a, g dbSNP:113472219
1060 1060 a, t dbSNP:201780596
1064 1064 c, t dbSNP:191438651
1065 1065 a, g dbSNP:200106123
1072 1072 c, t dbSNP:753797693
1074 1074 c, g dbSNP:772440416
1077 1077 c, t dbSNP:773830531
1080 1080 c, t dbSNP:539483296
1081 1081 a, c, t dbSNP:151256721
1082 1082 c, t dbSNP:759782726
1085 1085 c, t dbSNP:200348092
1095 1095 a, c dbSNP:752693628
1100 1100 g, t dbSNP:758563128
1104 1104 c, t dbSNP:777566694
1111 1111 a, g dbSNP:751517268
1122 1122 c, t dbSNP:757059123
1131 1131 a, g dbSNP:201966461
1133 1133 a, c dbSNP:745479177
1148 1148 c, t dbSNP:769541102
1151 1151 a, g dbSNP:779805206
1152 1152 a, g dbSNP:150841343
1153 1153 c, g dbSNP:768282879
1156 1156 a, g dbSNP:773529111
1161 1161 c, t dbSNP:375339833
1162 1162 a, g dbSNP:370506923
1165 1165 c, g dbSNP:182859275
1169 1169 a, g dbSNP:574173340
1178 1178 a, t dbSNP:544769405
1180 1180 a, g dbSNP:563405185
1214 1214 c, t dbSNP:778957473
1263 1263 a, t dbSNP:750265006
1289 1289 a, g dbSNP:530610327
1332 1332 a, g dbSNP:138224344
1337 1337 -, c dbSNP:780494436
1342 1342 a, g dbSNP:576867305
1352 1352 c, g dbSNP:564072310
1386 1386 c, t dbSNP:369583169
1392 1392 a, g dbSNP:574168586
1396 1396 c, g dbSNP:2234315
1407 1407 a, g dbSNP:761187027
1432 1432 a, g dbSNP:771242535
1444 1444 a, g dbSNP:776945507
1455 1455 a, g dbSNP:759938430
1458 1458 c, t dbSNP:765363258
1464 1464 c, t dbSNP:753029496
1465 1465 -, cg dbSNP:761081443
1465 1465 a, c dbSNP:762872607
1466 1466 a, g, t dbSNP:140477675
1467 1467 a, c, t dbSNP:529106769
1468 1468 a, g dbSNP:746937698
1469 1469 a, g dbSNP:528104106
1472 1472 a, g dbSNP:779572025
1473 1473 a, g dbSNP:550737419
1479 1479 c, g, t dbSNP:754391237
1488 1488 c, t dbSNP:569051610
1489 1489 a, g dbSNP:539543556
1492 1492 c, t dbSNP:147063227
1493 1493 a, c, g dbSNP:199740795
1495 1495 g, t dbSNP:775515733
1497 1497 -, tc dbSNP:779859900
1498 1498 c, t dbSNP:369421182
1507 1507 g, t dbSNP:747561622
1508 1508 g, t dbSNP:764042275
1510 1510 c, t dbSNP:774542819
1511 1511 a, g dbSNP:372687754
1522 1522 c, g dbSNP:767549989
1527 1527 c, t dbSNP:376926277
1532 1532 c, t dbSNP:755802675
1533 1533 a, t dbSNP:566536953
1536 1536 a, c dbSNP:766213741
1546 1546 c, t dbSNP:753386633
1549 1549 c, t dbSNP:541210117
1550 1550 a, g dbSNP:2234316
1551 1551 g, t dbSNP:747639120
1553 1553 a, g dbSNP:759992010
1591 1591 a, g dbSNP:555813172
1612 1612 a, g dbSNP:772548532
1620 1620 a, t dbSNP:2651818
1689 1689 c, t dbSNP:574259493
1698 1698 a, g dbSNP:544518851
1770 1770 c, t dbSNP:776203199
1771 1771 c, t dbSNP:188723879

Target ORF information:

RefSeq Version XM_011519809
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519809

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGCAGCCTA CCTCCTGGGC AGTGAGCTGC GGTCTGAGGC CCCTGCCCAG CTGGAAACCA 
CAGGGAGGGG AAGGGAGGGG AGGAGAGGAG AGGAGAGGAA CCGTCATGGG GCCTTGGAGT
CGAGTCAGGG TTGCCAAATG CCAGATGCTG GTCACCTGCT TCTTTATCTT GCTGCTGGGC
CTCTCTGTGG CCACCATGGT GACTCTTACC TACTTCGGGG CCCACTTTGC TGTCATCCGC
CGAGCGTCCC TGGAGAAGAA CCCGTACCAG GCTGTGCACC AATGGGGGAC TCAGCAGCGA
CTTATCCAAC ATCCAGAGAG CGGGAGCGAG GGCCAGAGCC TGCTGGGGCC ACTCAGGGCC
TTCTCTGCGG GGTTGAGCCT GGTGGGCCTC CTGACTCTGG GAGCCGTGCT GAGCGCTGCA
GCCACCGTGA GGGAGGCCCA GGGCCTCATG GCAGGGGGCT TCCTGTGCTT CTCCCTGGCG
TTCTGCGCAC AGGTGCAGGT GGTGTTCTGG AGACTCCACA GCCCCACCCA GGTGGAGGAC
GCCATGCTGG ACACCTACGA CCTGGTATAT GAGCAGGCGA TGAAAGGTAC GTCCCACGTC
CGGCGGCAGG AGCTGGCGGC CATCCAGGAC GTGTTTCTGT GCTGTGGGAA GAAGTCTCCT
TTCAGCCGTC TGGGGAGCAC AGAGGCTGAC CTGTGTCAGG GAGAGGAGGC GGCGAGAGAG
GACTGCCTTC AGGGCATCCG GAGCTTCCTG AGGACACACC AGCAGGTCGC CTCCAGCCTG
ACCAGCATCG GCCTGGCCCT CACGGTGTCC GCCTTGCTCT TCAGCTCCTT CCTGTGGTTT
GCCATCCGCT GTGGCTGCAG CTTGGACCGC AAGGGCAAAT ACACCCTGAC CCCACGAGCA
TGTGGCCGCC AGCCCCAGGA GCCCAGCCTC TTGAGATGCT CCCAGGGTGG ACCCACACAT
TGTCTCCACT CCGAAGCAGT TGCTATTGGT CCAAGAGGAT GCTCGGGTAG TCTTCGGTGG
CTGCAGGAGA GCGATGCTGC GCCTCTGCCC CTCTCCTGCC ACCTGGCTGC CCACAGAGGT
GAAGACGCCC CTGCTGTCAG CCCTCATGGG ATCCCTGAGG GGAGGGTCCG AGCTGTGAGG
AGGGAAGGGA GTGAAGGCCC AGCCAGAGAG CCAGGCTCCA TTGGGAACAG ATGCAAGGGT
AAGGGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63663
Accession Version XM_011519810.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1191bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tetraspanin-32 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGAGATA CCGAGGCTGG TAGCAGGCAG GTCCTCTGGC TGAGCTCTGC AGGGGGCCTG 
CTGTGCAGTT TCTTGAGCTG TGCTGGCAGC CTGAGTGTGG TGGTCCCCAC CGTGGTTTGC
AAATGGGGGG ACTCAGGCCC TCTGGGGGTG GGGGGAGCTC AAGGTTACCC TGGCAGTGCC
GGGGCTGGAT GGGGGCTCCA GGCTTACGAC AAAGGCTCTT GGCCCCAAAG TGCCCACCCA
CCCCTGGCAT CATTTGGGAG GAACCGCCTG AACCAGGTGG GAGAAACACC ATTTTATCAG
GCCCAGAAGG ATCCCAGAGG GGCTGAGCCC CCAGAAGAGG GCTGTGGCTT TGAGGACTGG
CACAGGAGTC TTACCAGGGT GGTGAGCTGG GCCAGGGCCC ACCAGAGCCG AATGGGCTTC
AACCTTGGGC TCCCTGTCCA ACAAAGTCCT GCTGGCAGCC TAGACAGTGG CAAAGGCCAA
AGGCCCCAAG CTGTTGGCAC CGGAAACGTC GAGGTGGAGG ACGCCATGCT GGACACCTAC
GACCTGGTAT ATGAGCAGGC GATGAAAGGT ACGTCCCACG TCCGGCGGCA GGAGCTGGCG
GCCATCCAGG ACGTGTTTCT GTGCTGTGGG AAGAAGTCTC CTTTCAGCCG TCTGGGGAGC
ACAGAGGCTG ACCTGTGTCA GGGAGAGGAG GCGGCGAGAG AGGACTGCCT TCAGGGCATC
CGGAGCTTCC TGAGGACACA CCAGCAGGTC GCCTCCAGCC TGACCAGCAT CGGCCTGGCC
CTCACGGTGT CCGCCTTGCT CTTCAGCTCC TTCCTGTGGT TTGCCATCCG CTGTGGCTGC
AGCTTGGACC GCAAGGGCAA ATACACCCTG ACCCCACGAG CATGTGGCCG CCAGCCCCAG
GAGCCCAGCC TCTTGAGATG CTCCCAGGGT GGACCCACAC ATTGTCTCCA CTCCGAAGCA
GTTGCTATTG GTCCAAGAGG ATGCTCGGGT AGTCTTCGGT GGCTGCAGGA GAGCGATGCT
GCGCCTCTGC CCCTCTCCTG CCACCTGGCT GCCCACAGAG GTGAAGACGC CCCTGCTGTC
AGCCCTCATG GGATCCCTGA GGGGAGGGTC CGAGCTGTGA GGAGGGAAGG GAGTGAAGGC
CCAGCCAGAG AGCCAGGCTC CATTGGGAAC AGATGCAAGG GTAAGGGGTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011518112.1
CDS932..2122
Misc Feature(1)1442..1675(+)
Misc Feature(2)1445..1549(+)
Translation
Position Chain Variation Link
2 2 -, ggg dbSNP:777634526
4 4 a, g dbSNP:371953629
5 5 c, t dbSNP:757112917
6 6 a, c, g dbSNP:780797896
7 7 a, t dbSNP:769288063
10 10 c, t dbSNP:779543727
30 30 c, t dbSNP:748595793
31 31 a, g dbSNP:772487085
34 34 g, t dbSNP:773810149
40 40 c, t dbSNP:760927991
49 49 g, t dbSNP:375443053
62 62 c, g, t dbSNP:78465685
63 63 a, g dbSNP:564849421
70 70 a, g dbSNP:765354088
78 78 a, c dbSNP:372594309
82 82 a, g dbSNP:762904620
95 95 g, t dbSNP:763904934
107 107 a, g dbSNP:375516709
111 111 -, gagga dbSNP:746834021
112 112 -, gagga dbSNP:770612134
131 131 a, g dbSNP:756986960
133 133 a, c dbSNP:781030842
134 134 c, t dbSNP:750010162
135 135 a, g dbSNP:532243385
144 144 a, c dbSNP:149680436
147 147 c, t dbSNP:748716777
153 153 c, t dbSNP:145517785
154 154 a, g dbSNP:148861275
154 154 -, g dbSNP:776304562
162 162 g, t dbSNP:778131898
173 173 a, c dbSNP:747621957
176 176 c, g dbSNP:142403358
181 181 a, c, t dbSNP:777152824
182 182 a, g dbSNP:770167215
195 195 g, t dbSNP:775892076
203 203 a, g dbSNP:762917262
204 204 a, c dbSNP:200210112
211 211 a, g dbSNP:758018002
218 218 g, t dbSNP:543107439
220 220 c, t dbSNP:746432153
225 225 a, t dbSNP:756540607
231 231 g, t dbSNP:780470109
235 235 c, t dbSNP:749412499
242 242 c, t dbSNP:768944967
248 248 c, t dbSNP:146480945
249 249 -, g dbSNP:750786852
253 253 c, t dbSNP:369696526
260 260 c, g, t dbSNP:576767301
268 268 c, t dbSNP:540982577
270 270 a, c, t dbSNP:140709712
271 271 a, g dbSNP:201106606
273 273 a, c, t dbSNP:559404880
274 274 a, g, t dbSNP:371441475
277 277 c, t dbSNP:529974468
278 278 a, g dbSNP:368347803
282 282 c, g dbSNP:762542013
295 295 c, t dbSNP:377338457
299 299 c, t dbSNP:750896014
300 300 a, c dbSNP:756704119
303 303 c, g dbSNP:145905096
304 304 a, c dbSNP:770456957
311 311 a, c dbSNP:755347022
313 313 a, c dbSNP:181067559
314 314 a, g dbSNP:531066922
318 318 a, g dbSNP:748434290
358 358 g, t dbSNP:371482652
371 371 c, t dbSNP:1501467
391 391 c, g dbSNP:774188366
399 399 a, g dbSNP:761371873
401 401 a, g dbSNP:201530910
403 403 a, g dbSNP:777126996
411 411 c, t dbSNP:759972432
412 412 c, t dbSNP:376702018
413 413 c, g dbSNP:765534620
421 421 c, t dbSNP:753089277
424 424 c, t dbSNP:138170136
428 428 g, t dbSNP:555848083
431 431 a, g dbSNP:371071821
432 432 a, g dbSNP:200631781
433 433 c, g dbSNP:751841969
438 438 a, g dbSNP:2234296
446 446 a, c, t dbSNP:199650987
447 447 a, c, g dbSNP:201634419
450 450 g, t dbSNP:749060868
459 459 a, g dbSNP:545681469
464 464 a, g dbSNP:774141716
465 465 a, g dbSNP:747759649
468 468 g, t dbSNP:200624662
476 476 c, t dbSNP:772617887
479 479 c, t dbSNP:760045340
481 481 c, t dbSNP:61744929
485 485 a, g dbSNP:776021550
488 488 a, g dbSNP:763294199
495 495 a, c dbSNP:768954809
506 506 c, t dbSNP:774603549
507 507 a, c dbSNP:372246596
511 511 c, t dbSNP:767604508
512 512 a, g dbSNP:570557887
518 518 a, c, g, t dbSNP:11554947
519 519 a, g dbSNP:770634605
525 525 g, t dbSNP:753770845
528 528 c, t dbSNP:754985828
539 539 a, c dbSNP:778727393
542 542 a, g dbSNP:752399702
543 543 a, g dbSNP:758260127
558 558 a, g dbSNP:376562078
566 566 c, t dbSNP:528240845
576 576 c, t dbSNP:2521275
600 600 a, g dbSNP:752733439
609 609 c, t dbSNP:7112919
610 610 a, g dbSNP:777632536
622 622 c, t dbSNP:751158881
623 623 a, g dbSNP:528912518
633 633 a, g dbSNP:550278120
635 635 c, g dbSNP:780644346
638 638 c, g dbSNP:745352695
647 647 a, c dbSNP:151205868
660 660 g, t dbSNP:139190458
667 667 a, g dbSNP:551600455
764 764 c, t dbSNP:566554119
778 778 c, t dbSNP:373427976
779 779 a, g dbSNP:112980712
814 814 a, g dbSNP:78942521
834 834 a, g dbSNP:573771318
841 841 a, g dbSNP:537879192
864 864 c, g dbSNP:576721965
867 867 a, g dbSNP:758943586
872 872 c, t dbSNP:556270437
878 878 a, g dbSNP:149964124
882 882 c, t dbSNP:545373281
883 883 a, g dbSNP:560789665
885 885 a, g dbSNP:572684750
886 886 c, t dbSNP:543206256
895 895 g, t dbSNP:561786930
909 909 a, g dbSNP:183135508
932 932 a, c dbSNP:74048246
943 943 c, t dbSNP:2651821
985 985 a, g dbSNP:559110012
988 988 c, t dbSNP:532894726
990 990 c, t dbSNP:2521276
1010 1010 c, t dbSNP:566394581
1061 1061 aa, gac dbSNP:386749795
1063 1063 a, c dbSNP:2521277
1066 1066 a, g dbSNP:2251711
1073 1073 c, t dbSNP:769753852
1081 1081 a, g dbSNP:567457738
1109 1109 a, g dbSNP:2521278
1112 1112 a, g dbSNP:556566268
1115 1115 a, g dbSNP:571552348
1139 1139 a, g dbSNP:538981067
1227 1227 a, c dbSNP:2521279
1244 1244 a, c dbSNP:572748288
1245 1245 c, g dbSNP:543271557
1259 1259 a, c dbSNP:555185425
1292 1292 c, g dbSNP:573818963
1320 1320 c, g dbSNP:2521280
1321 1321 a, g dbSNP:770364730
1385 1385 g, t dbSNP:560867873
1428 1428 a, g dbSNP:376694207
1433 1433 a, g dbSNP:752103948
1439 1439 a, g dbSNP:576261484
1448 1448 c, g dbSNP:775228702
1450 1450 a, g dbSNP:367966944
1453 1453 c, t dbSNP:2234300
1454 1454 a, g dbSNP:2234301
1466 1466 -, acctacg dbSNP:779134770
1467 1467 c, g dbSNP:757085417
1470 1470 a, g dbSNP:767136719
1471 1471 c, t dbSNP:147630481
1475 1475 a, c dbSNP:755576262
1477 1477 a, g dbSNP:779628656
1486 1486 a, g dbSNP:748555005
1491 1491 c, t dbSNP:758973942
1492 1492 a, g dbSNP:778462230
1500 1500 g, t dbSNP:112455917
1503 1503 c, t dbSNP:554395460
1510 1510 c, t dbSNP:368872715
1514 1514 c, t dbSNP:374473348
1515 1515 a, g dbSNP:138129469
1518 1518 a, g dbSNP:2234302
1530 1530 c, t dbSNP:561781505
1531 1531 a, g dbSNP:764063848
1543 1543 c, t dbSNP:541981635
1544 1544 a, g dbSNP:759043414
1547 1547 c, t dbSNP:772911765
1550 1550 c, t dbSNP:771804666
1555 1555 c, t dbSNP:773022730
1564 1564 a, g dbSNP:760426076
1571 1571 a, c dbSNP:147219779
1580 1580 c, t dbSNP:148601311
1581 1581 a, g dbSNP:142056890
1588 1588 a, g dbSNP:764840660
1596 1596 a, t dbSNP:752051464
1597 1597 a, g dbSNP:757864779
1598 1598 -, gct dbSNP:752515915
1609 1609 c, g, t dbSNP:781658568
1612 1612 a, g dbSNP:756488352
1623 1623 a, c, t dbSNP:780310923
1624 1624 a, g dbSNP:768658357
1626 1626 c, t dbSNP:778979541
1627 1627 a, g dbSNP:200478299
1637 1637 g, t dbSNP:771679154
1638 1638 a, g dbSNP:763277110
1640 1640 c, t dbSNP:777055649
1646 1646 a, g dbSNP:760046572
1652 1652 a, c, t dbSNP:376763237
1653 1653 a, g dbSNP:766761476
1654 1654 a, g dbSNP:186572696
1656 1656 a, g dbSNP:764203991
1678 1678 g, t dbSNP:369033190
1679 1679 a, g dbSNP:761799293
1681 1681 c, t dbSNP:150731413
1682 1682 a, g dbSNP:371671456
1683 1683 c, t dbSNP:756144294
1696 1696 -, cag dbSNP:774995039
1703 1703 a, g dbSNP:779955859
1704 1704 a, g dbSNP:753662640
1709 1709 g, t dbSNP:201400387
1712 1712 c, g, t dbSNP:778426094
1716 1716 c, t dbSNP:771421230
1717 1717 a, g dbSNP:777282787
1719 1719 -, gtcc dbSNP:772632958
1723 1723 c, t dbSNP:553461987
1724 1724 a, g dbSNP:778304554
1726 1726 c, g dbSNP:747184229
1732 1732 c, g dbSNP:200678268
1734 1734 g, t dbSNP:781315038
1738 1738 c, t dbSNP:745944280
1747 1747 a, g dbSNP:769833899
1758 1758 c, t dbSNP:374327578
1760 1760 a, c, t dbSNP:200026082
1761 1761 a, g dbSNP:774163392
1768 1768 c, t dbSNP:369812524
1770 1770 a, g dbSNP:143112175
1772 1772 a, g dbSNP:561690224
1781 1781 c, t dbSNP:767178490
1782 1782 a, c, g, t dbSNP:750103889
1797 1797 a, c dbSNP:753206297
1805 1805 c, g, t dbSNP:377055075
1808 1808 c, t dbSNP:751947184
1809 1809 a, g dbSNP:371452736
1812 1812 c, t dbSNP:756317638
1820 1820 c, t dbSNP:375291522
1821 1821 a, g dbSNP:756322762
1823 1823 c, g dbSNP:780299105
1828 1828 c, g, t dbSNP:749352222
1851 1851 g, t dbSNP:3180160
1857 1857 a, t dbSNP:139081815
1862 1862 a, g dbSNP:778820239
1865 1865 -, c dbSNP:773635676
1869 1869 c, t dbSNP:144063439
1875 1875 a, g, t dbSNP:146837754
1885 1885 c, t dbSNP:140677453
1886 1886 a, g dbSNP:770684289
1893 1893 a, t dbSNP:776483823
1898 1898 a, g dbSNP:759111860
1901 1901 a, g dbSNP:764842683
1902 1902 a, g dbSNP:774943231
1904 1904 c, t dbSNP:762250888
1907 1907 a, c, g dbSNP:144460879
1908 1908 c, g dbSNP:367851224
1913 1913 c, t dbSNP:34745234
1917 1917 c, t dbSNP:149230396
1918 1918 a, c, g dbSNP:371556972
1922 1922 a, g dbSNP:752637049
1924 1924 c, t dbSNP:758233212
1925 1925 a, c dbSNP:777814788
1928 1928 c, t dbSNP:143225348
1929 1929 a, c, g dbSNP:375834458
1938 1938 a, g dbSNP:113472219
1941 1941 a, t dbSNP:201780596
1945 1945 c, t dbSNP:191438651
1946 1946 a, g dbSNP:200106123
1953 1953 c, t dbSNP:753797693
1955 1955 c, g dbSNP:772440416
1958 1958 c, t dbSNP:773830531
1961 1961 c, t dbSNP:539483296
1962 1962 a, c, t dbSNP:151256721
1963 1963 c, t dbSNP:759782726
1966 1966 c, t dbSNP:200348092
1976 1976 a, c dbSNP:752693628
1981 1981 g, t dbSNP:758563128
1985 1985 c, t dbSNP:777566694
1992 1992 a, g dbSNP:751517268
2003 2003 c, t dbSNP:757059123
2012 2012 a, g dbSNP:201966461
2014 2014 a, c dbSNP:745479177
2029 2029 c, t dbSNP:769541102
2032 2032 a, g dbSNP:779805206
2033 2033 a, g dbSNP:150841343
2034 2034 c, g dbSNP:768282879
2037 2037 a, g dbSNP:773529111
2042 2042 c, t dbSNP:375339833
2043 2043 a, g dbSNP:370506923
2046 2046 c, g dbSNP:182859275
2050 2050 a, g dbSNP:574173340
2059 2059 a, t dbSNP:544769405
2061 2061 a, g dbSNP:563405185
2095 2095 c, t dbSNP:778957473
2144 2144 a, t dbSNP:750265006
2170 2170 a, g dbSNP:530610327
2213 2213 a, g dbSNP:138224344
2218 2218 -, c dbSNP:780494436
2223 2223 a, g dbSNP:576867305
2233 2233 c, g dbSNP:564072310
2267 2267 c, t dbSNP:369583169
2273 2273 a, g dbSNP:574168586
2277 2277 c, g dbSNP:2234315
2288 2288 a, g dbSNP:761187027
2313 2313 a, g dbSNP:771242535
2325 2325 a, g dbSNP:776945507
2336 2336 a, g dbSNP:759938430
2339 2339 c, t dbSNP:765363258
2345 2345 c, t dbSNP:753029496
2346 2346 -, cg dbSNP:761081443
2346 2346 a, c dbSNP:762872607
2347 2347 a, g, t dbSNP:140477675
2348 2348 a, c, t dbSNP:529106769
2349 2349 a, g dbSNP:746937698
2350 2350 a, g dbSNP:528104106
2353 2353 a, g dbSNP:779572025
2354 2354 a, g dbSNP:550737419
2360 2360 c, g, t dbSNP:754391237
2369 2369 c, t dbSNP:569051610
2370 2370 a, g dbSNP:539543556
2373 2373 c, t dbSNP:147063227
2374 2374 a, c, g dbSNP:199740795
2376 2376 g, t dbSNP:775515733
2378 2378 -, tc dbSNP:779859900
2379 2379 c, t dbSNP:369421182
2388 2388 g, t dbSNP:747561622
2389 2389 g, t dbSNP:764042275
2391 2391 c, t dbSNP:774542819
2392 2392 a, g dbSNP:372687754
2403 2403 c, g dbSNP:767549989
2408 2408 c, t dbSNP:376926277
2413 2413 c, t dbSNP:755802675
2414 2414 a, t dbSNP:566536953
2417 2417 a, c dbSNP:766213741
2427 2427 c, t dbSNP:753386633
2430 2430 c, t dbSNP:541210117
2431 2431 a, g dbSNP:2234316
2432 2432 g, t dbSNP:747639120
2434 2434 a, g dbSNP:759992010
2472 2472 a, g dbSNP:555813172
2493 2493 a, g dbSNP:772548532
2501 2501 a, t dbSNP:2651818
2570 2570 c, t dbSNP:574259493
2579 2579 a, g dbSNP:544518851
2651 2651 c, t dbSNP:776203199
2652 2652 c, t dbSNP:188723879

Target ORF information:

RefSeq Version XM_011519810
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519810

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGAGAGATA CCGAGGCTGG TAGCAGGCAG GTCCTCTGGC TGAGCTCTGC AGGGGGCCTG 
CTGTGCAGTT TCTTGAGCTG TGCTGGCAGC CTGAGTGTGG TGGTCCCCAC CGTGGTTTGC
AAATGGGGGG ACTCAGGCCC TCTGGGGGTG GGGGGAGCTC AAGGTTACCC TGGCAGTGCC
GGGGCTGGAT GGGGGCTCCA GGCTTACGAC AAAGGCTCTT GGCCCCAAAG TGCCCACCCA
CCCCTGGCAT CATTTGGGAG GAACCGCCTG AACCAGGTGG GAGAAACACC ATTTTATCAG
GCCCAGAAGG ATCCCAGAGG GGCTGAGCCC CCAGAAGAGG GCTGTGGCTT TGAGGACTGG
CACAGGAGTC TTACCAGGGT GGTGAGCTGG GCCAGGGCCC ACCAGAGCCG AATGGGCTTC
AACCTTGGGC TCCCTGTCCA ACAAAGTCCT GCTGGCAGCC TAGACAGTGG CAAAGGCCAA
AGGCCCCAAG CTGTTGGCAC CGGAAACGTC GAGGTGGAGG ACGCCATGCT GGACACCTAC
GACCTGGTAT ATGAGCAGGC GATGAAAGGT ACGTCCCACG TCCGGCGGCA GGAGCTGGCG
GCCATCCAGG ACGTGTTTCT GTGCTGTGGG AAGAAGTCTC CTTTCAGCCG TCTGGGGAGC
ACAGAGGCTG ACCTGTGTCA GGGAGAGGAG GCGGCGAGAG AGGACTGCCT TCAGGGCATC
CGGAGCTTCC TGAGGACACA CCAGCAGGTC GCCTCCAGCC TGACCAGCAT CGGCCTGGCC
CTCACGGTGT CCGCCTTGCT CTTCAGCTCC TTCCTGTGGT TTGCCATCCG CTGTGGCTGC
AGCTTGGACC GCAAGGGCAA ATACACCCTG ACCCCACGAG CATGTGGCCG CCAGCCCCAG
GAGCCCAGCC TCTTGAGATG CTCCCAGGGT GGACCCACAC ATTGTCTCCA CTCCGAAGCA
GTTGCTATTG GTCCAAGAGG ATGCTCGGGT AGTCTTCGGT GGCTGCAGGA GAGCGATGCT
GCGCCTCTGC CCCTCTCCTG CCACCTGGCT GCCCACAGAG GTGAAGACGC CCCTGCTGTC
AGCCCTCATG GGATCCCTGA GGGGAGGGTC CGAGCTGTGA GGAGGGAAGG GAGTGAAGGC
CCAGCCAGAG AGCCAGGCTC CATTGGGAAC AGATGCAAGG GTAAGGGGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63664
Accession Version XM_011519811.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1140bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tetraspanin-32 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGCCTA CCTCCTGGGC AGTGAGCTGC GGTCTGAGGC CCCTGCCCAG CTGGAAACCA 
CAGGGAGGGG AAGGGAGGGG AGGAGAGGAG AGGAGAGGAA CCGTCATGGG GCCTTGGAGT
CGAGTCAGGG TTGCCAAATG CCAGATGCTG GTCACCTGCT TCTTTATCTT GCTGCTGGGC
CTCTCTGTGG CCACCATGGT GACTCTTACC TACTTCGGGG CCCACTTTGC TGTCATCCGC
CGAGCGTCCC TGGAGAAGAA CCCGTACCAG GCTGTGCACC AATGGGGGAC TCAGCAGCGA
CTTATCCAAC ATCCAGAGAG CGGGAGCGAG GGCCAGAGCC TGCTGGGGCC ACTCAGGGCC
TTCTCTGCGG GGTTGAGCCT GGTGGGCCTC CTGACTCTGG GAGCCGTGCT GAGCGCTGCA
GCCACCGTGA GGGAGGCCCA GGGCCTCATG GCAGGGGGCT TCCTGTGCTT CTCCCTGGCG
TTCTGCGCAC AGGTGCAGGT GGTGTTCTGG AGACTCCACA GCCCCACCCA GGTGGAGGAC
GCCATGCTGG ACACCTACGA CCTGGTATAT GAGCAGGCGA TGAAAGGTAC GTCCCACGTC
CGGCGGCAGG AGCTGGCGGC CATCCAGGAC GTGTTTCTGT GCTGTGGGAA GAAGTCTCCT
TTCAGCCGTC TGGGGAGCAC AGAGGCTGAC CTGTGTCAGG GAGAGGAGGC GGCGAGAGAG
GACTGCCTTC AGGGCATCCG GAGCTTCCTG AGGACACACC AGCAGGTCGC CTCCAGCCTG
ACCAGCATCG GCCTGGCCCT CACGGTGTCC GCCTTGCTCT TCAGCTCCTT CCTGTGGTTT
GCCATCCGCT GTGGCTGCAG CTTGGACCGC AAGGGCAAAT ACACCCTGAC CCCACGAGCA
TGTGGCCGCC AGCCCCAGGA GCCCAGCCTC TTGAGATGCT CCCAGGGTGG ACCCACACAT
TGTCTCCACT CCGAAGCAGT TGCTATTGGT CCAAGAGGAT GCTCGGGTAG TCTTCGGTGG
CTGCAGGAGA GCGATGCTGC GCCTCTGCCC CTCTCCTGCC ACCTGGCTGC CCACAGAGCT
CTCCAGGGCA GAAGTCGCGG TGGGCTCAGT GGGTGCCCTG AGCGGGGTCT CTCAGACTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011518113.1
CDS33..1172
Misc Feature(1)369..878(+)
Misc Feature(2)546..794(+)
Misc Feature(3)549..668(+)
Translation
Position Chain Variation Link
2 2 -, ggg dbSNP:777634526
4 4 a, g dbSNP:371953629
5 5 c, t dbSNP:757112917
6 6 a, c, g dbSNP:780797896
7 7 a, t dbSNP:769288063
10 10 c, t dbSNP:779543727
30 30 c, t dbSNP:748595793
31 31 a, g dbSNP:772487085
34 34 g, t dbSNP:773810149
40 40 c, t dbSNP:760927991
49 49 g, t dbSNP:375443053
62 62 c, g, t dbSNP:78465685
63 63 a, g dbSNP:564849421
70 70 a, g dbSNP:765354088
78 78 a, c dbSNP:372594309
82 82 a, g dbSNP:762904620
95 95 g, t dbSNP:763904934
107 107 a, g dbSNP:375516709
111 111 -, gagga dbSNP:746834021
112 112 -, gagga dbSNP:770612134
131 131 a, g dbSNP:756986960
133 133 a, c dbSNP:781030842
134 134 c, t dbSNP:750010162
135 135 a, g dbSNP:532243385
144 144 a, c dbSNP:149680436
147 147 c, t dbSNP:748716777
153 153 c, t dbSNP:145517785
154 154 a, g dbSNP:148861275
154 154 -, g dbSNP:776304562
162 162 g, t dbSNP:778131898
173 173 a, c dbSNP:747621957
176 176 c, g dbSNP:142403358
181 181 a, c, t dbSNP:777152824
182 182 a, g dbSNP:770167215
195 195 g, t dbSNP:775892076
203 203 a, g dbSNP:762917262
204 204 a, c dbSNP:200210112
211 211 a, g dbSNP:758018002
218 218 g, t dbSNP:543107439
220 220 c, t dbSNP:746432153
225 225 a, t dbSNP:756540607
231 231 g, t dbSNP:780470109
235 235 c, t dbSNP:749412499
242 242 c, t dbSNP:768944967
248 248 c, t dbSNP:146480945
249 249 -, g dbSNP:750786852
253 253 c, t dbSNP:369696526
260 260 c, g, t dbSNP:576767301
268 268 c, t dbSNP:540982577
270 270 a, c, t dbSNP:140709712
271 271 a, g dbSNP:201106606
273 273 a, c, t dbSNP:559404880
274 274 a, g, t dbSNP:371441475
277 277 c, t dbSNP:529974468
278 278 a, g dbSNP:368347803
282 282 c, g dbSNP:762542013
295 295 c, t dbSNP:377338457
299 299 c, t dbSNP:750896014
300 300 a, c dbSNP:756704119
303 303 c, g dbSNP:145905096
304 304 a, c dbSNP:770456957
311 311 a, c dbSNP:755347022
313 313 a, c dbSNP:181067559
314 314 a, g dbSNP:531066922
318 318 a, g dbSNP:748434290
358 358 g, t dbSNP:371482652
371 371 c, t dbSNP:1501467
391 391 c, g dbSNP:774188366
399 399 a, g dbSNP:761371873
401 401 a, g dbSNP:201530910
403 403 a, g dbSNP:777126996
411 411 c, t dbSNP:759972432
412 412 c, t dbSNP:376702018
413 413 c, g dbSNP:765534620
421 421 c, t dbSNP:753089277
424 424 c, t dbSNP:138170136
428 428 g, t dbSNP:555848083
431 431 a, g dbSNP:371071821
432 432 a, g dbSNP:200631781
433 433 c, g dbSNP:751841969
438 438 a, g dbSNP:2234296
446 446 a, c, t dbSNP:199650987
447 447 a, c, g dbSNP:201634419
450 450 g, t dbSNP:749060868
459 459 a, g dbSNP:545681469
464 464 a, g dbSNP:774141716
465 465 a, g dbSNP:747759649
468 468 g, t dbSNP:200624662
476 476 c, t dbSNP:772617887
479 479 c, t dbSNP:760045340
481 481 c, t dbSNP:61744929
485 485 a, g dbSNP:776021550
488 488 a, g dbSNP:763294199
495 495 a, c dbSNP:768954809
506 506 c, t dbSNP:774603549
507 507 a, c dbSNP:372246596
511 511 c, t dbSNP:767604508
512 512 a, g dbSNP:570557887
518 518 a, c, g, t dbSNP:11554947
519 519 a, g dbSNP:770634605
525 525 g, t dbSNP:753770845
528 528 c, t dbSNP:754985828
539 539 a, c dbSNP:778727393
542 542 a, g dbSNP:752399702
543 543 a, g dbSNP:758260127
558 558 a, g dbSNP:376562078
567 567 c, g dbSNP:775228702
569 569 a, g dbSNP:367966944
572 572 c, t dbSNP:2234300
573 573 a, g dbSNP:2234301
585 585 -, acctacg dbSNP:779134770
586 586 c, g dbSNP:757085417
589 589 a, g dbSNP:767136719
590 590 c, t dbSNP:147630481
594 594 a, c dbSNP:755576262
596 596 a, g dbSNP:779628656
605 605 a, g dbSNP:748555005
610 610 c, t dbSNP:758973942
611 611 a, g dbSNP:778462230
619 619 g, t dbSNP:112455917
622 622 c, t dbSNP:554395460
629 629 c, t dbSNP:368872715
633 633 c, t dbSNP:374473348
634 634 a, g dbSNP:138129469
637 637 a, g dbSNP:2234302
649 649 c, t dbSNP:561781505
650 650 a, g dbSNP:764063848
662 662 c, t dbSNP:541981635
663 663 a, g dbSNP:759043414
666 666 c, t dbSNP:772911765
669 669 c, t dbSNP:771804666
674 674 c, t dbSNP:773022730
683 683 a, g dbSNP:760426076
690 690 a, c dbSNP:147219779
699 699 c, t dbSNP:148601311
700 700 a, g dbSNP:142056890
707 707 a, g dbSNP:764840660
715 715 a, t dbSNP:752051464
716 716 a, g dbSNP:757864779
717 717 -, gct dbSNP:752515915
728 728 c, g, t dbSNP:781658568
731 731 a, g dbSNP:756488352
742 742 a, c, t dbSNP:780310923
743 743 a, g dbSNP:768658357
745 745 c, t dbSNP:778979541
746 746 a, g dbSNP:200478299
756 756 g, t dbSNP:771679154
757 757 a, g dbSNP:763277110
759 759 c, t dbSNP:777055649
765 765 a, g dbSNP:760046572
771 771 a, c, t dbSNP:376763237
772 772 a, g dbSNP:766761476
773 773 a, g dbSNP:186572696
775 775 a, g dbSNP:764203991
797 797 g, t dbSNP:369033190
798 798 a, g dbSNP:761799293
800 800 c, t dbSNP:150731413
801 801 a, g dbSNP:371671456
802 802 c, t dbSNP:756144294
815 815 -, cag dbSNP:774995039
822 822 a, g dbSNP:779955859
823 823 a, g dbSNP:753662640
828 828 g, t dbSNP:201400387
831 831 c, g, t dbSNP:778426094
835 835 c, t dbSNP:771421230
836 836 a, g dbSNP:777282787
838 838 -, gtcc dbSNP:772632958
842 842 c, t dbSNP:553461987
843 843 a, g dbSNP:778304554
845 845 c, g dbSNP:747184229
851 851 c, g dbSNP:200678268
853 853 g, t dbSNP:781315038
857 857 c, t dbSNP:745944280
866 866 a, g dbSNP:769833899
877 877 c, t dbSNP:374327578
879 879 a, c, t dbSNP:200026082
880 880 a, g dbSNP:774163392
887 887 c, t dbSNP:369812524
889 889 a, g dbSNP:143112175
891 891 a, g dbSNP:561690224
900 900 c, t dbSNP:767178490
901 901 a, c, g, t dbSNP:750103889
916 916 a, c dbSNP:753206297
924 924 c, g, t dbSNP:377055075
927 927 c, t dbSNP:751947184
928 928 a, g dbSNP:371452736
931 931 c, t dbSNP:756317638
939 939 c, t dbSNP:375291522
940 940 a, g dbSNP:756322762
942 942 c, g dbSNP:780299105
947 947 c, g, t dbSNP:749352222
970 970 g, t dbSNP:3180160
976 976 a, t dbSNP:139081815
981 981 a, g dbSNP:778820239
984 984 -, c dbSNP:773635676
988 988 c, t dbSNP:144063439
994 994 a, g, t dbSNP:146837754
1004 1004 c, t dbSNP:140677453
1005 1005 a, g dbSNP:770684289
1012 1012 a, t dbSNP:776483823
1017 1017 a, g dbSNP:759111860
1020 1020 a, g dbSNP:764842683
1021 1021 a, g dbSNP:774943231
1023 1023 c, t dbSNP:762250888
1026 1026 a, c, g dbSNP:144460879
1027 1027 c, g dbSNP:367851224
1032 1032 c, t dbSNP:34745234
1036 1036 c, t dbSNP:149230396
1037 1037 a, c, g dbSNP:371556972
1041 1041 a, g dbSNP:752637049
1043 1043 c, t dbSNP:758233212
1044 1044 a, c dbSNP:777814788
1047 1047 c, t dbSNP:143225348
1048 1048 a, c, g dbSNP:375834458
1057 1057 a, g dbSNP:113472219
1060 1060 a, t dbSNP:201780596
1064 1064 c, t dbSNP:191438651
1065 1065 a, g dbSNP:200106123
1072 1072 c, t dbSNP:753797693
1074 1074 c, g dbSNP:772440416
1077 1077 c, t dbSNP:773830531
1080 1080 c, t dbSNP:539483296
1081 1081 a, c, t dbSNP:151256721
1082 1082 c, t dbSNP:759782726
1085 1085 c, t dbSNP:200348092
1095 1095 a, c dbSNP:752693628
1100 1100 g, t dbSNP:758563128
1104 1104 c, t dbSNP:777566694
1118 1118 a, g dbSNP:759938430
1121 1121 c, t dbSNP:765363258
1127 1127 c, t dbSNP:753029496
1128 1128 -, cg dbSNP:761081443
1128 1128 a, c dbSNP:762872607
1129 1129 a, g, t dbSNP:140477675
1130 1130 a, c, t dbSNP:529106769
1131 1131 a, g dbSNP:746937698
1132 1132 a, g dbSNP:528104106
1135 1135 a, g dbSNP:779572025
1136 1136 a, g dbSNP:550737419
1142 1142 c, g, t dbSNP:754391237
1151 1151 c, t dbSNP:569051610
1152 1152 a, g dbSNP:539543556
1155 1155 c, t dbSNP:147063227
1156 1156 a, c, g dbSNP:199740795
1158 1158 g, t dbSNP:775515733
1160 1160 -, tc dbSNP:779859900
1161 1161 c, t dbSNP:369421182
1170 1170 g, t dbSNP:747561622
1171 1171 g, t dbSNP:764042275
1173 1173 c, t dbSNP:774542819
1174 1174 a, g dbSNP:372687754
1185 1185 c, g dbSNP:767549989
1190 1190 c, t dbSNP:376926277
1195 1195 c, t dbSNP:755802675
1196 1196 a, t dbSNP:566536953
1199 1199 a, c dbSNP:766213741
1209 1209 c, t dbSNP:753386633
1212 1212 c, t dbSNP:541210117
1213 1213 a, g dbSNP:2234316
1214 1214 g, t dbSNP:747639120
1216 1216 a, g dbSNP:759992010
1254 1254 a, g dbSNP:555813172
1275 1275 a, g dbSNP:772548532
1283 1283 a, t dbSNP:2651818
1352 1352 c, t dbSNP:574259493
1361 1361 a, g dbSNP:544518851
1433 1433 c, t dbSNP:776203199
1434 1434 c, t dbSNP:188723879

Target ORF information:

RefSeq Version XM_011519811
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519811

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGCCTA CCTCCTGGGC AGTGAGCTGC GGTCTGAGGC CCCTGCCCAG CTGGAAACCA 
CAGGGAGGGG AAGGGAGGGG AGGAGAGGAG AGGAGAGGAA CCGTCATGGG GCCTTGGAGT
CGAGTCAGGG TTGCCAAATG CCAGATGCTG GTCACCTGCT TCTTTATCTT GCTGCTGGGC
CTCTCTGTGG CCACCATGGT GACTCTTACC TACTTCGGGG CCCACTTTGC TGTCATCCGC
CGAGCGTCCC TGGAGAAGAA CCCGTACCAG GCTGTGCACC AATGGGGGAC TCAGCAGCGA
CTTATCCAAC ATCCAGAGAG CGGGAGCGAG GGCCAGAGCC TGCTGGGGCC ACTCAGGGCC
TTCTCTGCGG GGTTGAGCCT GGTGGGCCTC CTGACTCTGG GAGCCGTGCT GAGCGCTGCA
GCCACCGTGA GGGAGGCCCA GGGCCTCATG GCAGGGGGCT TCCTGTGCTT CTCCCTGGCG
TTCTGCGCAC AGGTGCAGGT GGTGTTCTGG AGACTCCACA GCCCCACCCA GGTGGAGGAC
GCCATGCTGG ACACCTACGA CCTGGTATAT GAGCAGGCGA TGAAAGGTAC GTCCCACGTC
CGGCGGCAGG AGCTGGCGGC CATCCAGGAC GTGTTTCTGT GCTGTGGGAA GAAGTCTCCT
TTCAGCCGTC TGGGGAGCAC AGAGGCTGAC CTGTGTCAGG GAGAGGAGGC GGCGAGAGAG
GACTGCCTTC AGGGCATCCG GAGCTTCCTG AGGACACACC AGCAGGTCGC CTCCAGCCTG
ACCAGCATCG GCCTGGCCCT CACGGTGTCC GCCTTGCTCT TCAGCTCCTT CCTGTGGTTT
GCCATCCGCT GTGGCTGCAG CTTGGACCGC AAGGGCAAAT ACACCCTGAC CCCACGAGCA
TGTGGCCGCC AGCCCCAGGA GCCCAGCCTC TTGAGATGCT CCCAGGGTGG ACCCACACAT
TGTCTCCACT CCGAAGCAGT TGCTATTGGT CCAAGAGGAT GCTCGGGTAG TCTTCGGTGG
CTGCAGGAGA GCGATGCTGC GCCTCTGCCC CTCTCCTGCC ACCTGGCTGC CCACAGAGCT
CTCCAGGGCA GAAGTCGCGG TGGGCTCAGT GGGTGCCCTG AGCGGGGTCT CTCAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu44730
Accession Version XM_005252719.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1137bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tetraspanin-32 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578820258. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGCCTA CCTCCTGGGC AGTGAGCTGC GGTCTGAGGC CCCTGCCCAG CTGGAAACCA 
CAGGGAGGGG AAGGGAGGGG AGGAGAGGAG AGGAGAGGAA CCGTCATGGG GCCTTGGAGT
CGAGTCAGGG TTGCCAAATG CCAGATGCTG GTCACCTGCT TCTTTATCTT GCTGCTGGGC
CTCTCTGTGG CCACCATGGT GACTCTTACC TACTTCGGGG CCCACTTTGC TGTCATCCGC
CGAGCGTCCC TGGAGAAGAA CCCGTACCAG GCTGTGCACC AATGGGCCTT CTCTGCGGGG
TTGAGCCTGG TGGGCCTCCT GACTCTGGGA GCCGTGCTGA GCGCTGCAGC CACCGTGAGG
GAGGCCCAGG GCCTCATGGC AGGGGGCTTC CTGTGCTTCT CCCTGGCGTT CTGCGCACAG
GTGCAGGTGG TGTTCTGGAG ACTCCACAGC CCCACCCAGG TGGAGGACGC CATGCTGGAC
ACCTACGACC TGGTATATGA GCAGGCGATG AAAGGTACGT CCCACGTCCG GCGGCAGGAG
CTGGCGGCCA TCCAGGACGT GTTTCTGTGC TGTGGGAAGA AGTCTCCTTT CAGCCGTCTG
GGGAGCACAG AGGCTGACCT GTGTCAGGGA GAGGAGGCGG CGAGAGAGGA CTGCCTTCAG
GGCATCCGGA GCTTCCTGAG GACACACCAG CAGGTCGCCT CCAGCCTGAC CAGCATCGGC
CTGGCCCTCA CGGTGTCCGC CTTGCTCTTC AGCTCCTTCC TGTGGTTTGC CATCCGCTGT
GGCTGCAGCT TGGACCGCAA GGGCAAATAC ACCCTGACCC CACGAGCATG TGGCCGCCAG
CCCCAGGAGC CCAGCCTCTT GAGATGCTCC CAGGGTGGAC CCACACATTG TCTCCACTCC
GAAGCAGTTG CTATTGGTCC AAGAGGATGC TCGGGTAGTC TTCGGTGGCT GCAGGAGAGC
GATGCTGCGC CTCTGCCCCT CTCCTGCCAC CTGGCTGCCC ACAGAGGTGA AGACGCCCCT
GCTGTCAGCC CTCATGGGAT CCCTGAGGGG AGGGTCCGAG CTGTGAGGAG GGAAGGGAGT
GAAGGCCCAG CCAGAGAGCC AGGCTCCATT GGGAACAGAT GCAAGGGTAA GGGGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005252776.1
CDS28..1164
Misc Feature(1)163..801(+)
Misc Feature(2)469..717(+)
Misc Feature(3)472..591(+)
Translation
Position Chain Variation Link
1 1 a, c, g dbSNP:780797896
2 2 a, t dbSNP:769288063
5 5 c, t dbSNP:779543727
25 25 c, t dbSNP:748595793
26 26 a, g dbSNP:772487085
29 29 g, t dbSNP:773810149
35 35 c, t dbSNP:760927991
44 44 g, t dbSNP:375443053
57 57 c, g, t dbSNP:78465685
58 58 a, g dbSNP:564849421
65 65 a, g dbSNP:765354088
73 73 a, c dbSNP:372594309
77 77 a, g dbSNP:762904620
90 90 g, t dbSNP:763904934
102 102 a, g dbSNP:375516709
106 106 -, gagga dbSNP:746834021
107 107 -, gagga dbSNP:770612134
126 126 a, g dbSNP:756986960
128 128 a, c dbSNP:781030842
129 129 c, t dbSNP:750010162
130 130 a, g dbSNP:532243385
139 139 a, c dbSNP:149680436
142 142 c, t dbSNP:748716777
148 148 c, t dbSNP:145517785
149 149 a, g dbSNP:148861275
149 149 -, g dbSNP:776304562
157 157 g, t dbSNP:778131898
168 168 a, c dbSNP:747621957
171 171 c, g dbSNP:142403358
176 176 a, c, t dbSNP:777152824
177 177 a, g dbSNP:770167215
190 190 g, t dbSNP:775892076
198 198 a, g dbSNP:762917262
199 199 a, c dbSNP:200210112
206 206 a, g dbSNP:758018002
213 213 g, t dbSNP:543107439
215 215 c, t dbSNP:746432153
220 220 a, t dbSNP:756540607
226 226 g, t dbSNP:780470109
230 230 c, t dbSNP:749412499
237 237 c, t dbSNP:768944967
243 243 c, t dbSNP:146480945
244 244 -, g dbSNP:750786852
248 248 c, t dbSNP:369696526
255 255 c, g, t dbSNP:576767301
263 263 c, t dbSNP:540982577
265 265 a, c, t dbSNP:140709712
266 266 a, g dbSNP:201106606
268 268 a, c, t dbSNP:559404880
269 269 a, g, t dbSNP:371441475
272 272 c, t dbSNP:529974468
273 273 a, g dbSNP:368347803
277 277 c, g dbSNP:762542013
290 290 c, t dbSNP:377338457
294 294 c, t dbSNP:750896014
295 295 a, c dbSNP:756704119
298 298 c, g dbSNP:145905096
299 299 a, c dbSNP:770456957
306 306 a, c dbSNP:755347022
308 308 a, c dbSNP:181067559
309 309 a, g dbSNP:531066922
313 313 a, g dbSNP:748434290
314 314 c, g dbSNP:774188366
322 322 a, g dbSNP:761371873
324 324 a, g dbSNP:201530910
326 326 a, g dbSNP:777126996
334 334 c, t dbSNP:759972432
335 335 c, t dbSNP:376702018
336 336 c, g dbSNP:765534620
344 344 c, t dbSNP:753089277
347 347 c, t dbSNP:138170136
351 351 g, t dbSNP:555848083
354 354 a, g dbSNP:371071821
355 355 a, g dbSNP:200631781
356 356 c, g dbSNP:751841969
361 361 a, g dbSNP:2234296
369 369 a, c, t dbSNP:199650987
370 370 a, c, g dbSNP:201634419
373 373 g, t dbSNP:749060868
382 382 a, g dbSNP:545681469
387 387 a, g dbSNP:774141716
388 388 a, g dbSNP:747759649
391 391 g, t dbSNP:200624662
399 399 c, t dbSNP:772617887
402 402 c, t dbSNP:760045340
404 404 c, t dbSNP:61744929
408 408 a, g dbSNP:776021550
411 411 a, g dbSNP:763294199
418 418 a, c dbSNP:768954809
429 429 c, t dbSNP:774603549
430 430 a, c dbSNP:372246596
434 434 c, t dbSNP:767604508
435 435 a, g dbSNP:570557887
441 441 a, c, g, t dbSNP:11554947
442 442 a, g dbSNP:770634605
448 448 g, t dbSNP:753770845
451 451 c, t dbSNP:754985828
462 462 a, c dbSNP:778727393
465 465 a, g dbSNP:752399702
466 466 a, g dbSNP:758260127
481 481 a, g dbSNP:376562078
490 490 c, g dbSNP:775228702
492 492 a, g dbSNP:367966944
495 495 c, t dbSNP:2234300
496 496 a, g dbSNP:2234301
508 508 -, acctacg dbSNP:779134770
509 509 c, g dbSNP:757085417
512 512 a, g dbSNP:767136719
513 513 c, t dbSNP:147630481
517 517 a, c dbSNP:755576262
519 519 a, g dbSNP:779628656
528 528 a, g dbSNP:748555005
533 533 c, t dbSNP:758973942
534 534 a, g dbSNP:778462230
542 542 g, t dbSNP:112455917
545 545 c, t dbSNP:554395460
552 552 c, t dbSNP:368872715
556 556 c, t dbSNP:374473348
557 557 a, g dbSNP:138129469
560 560 a, g dbSNP:2234302
572 572 c, t dbSNP:561781505
573 573 a, g dbSNP:764063848
585 585 c, t dbSNP:541981635
586 586 a, g dbSNP:759043414
589 589 c, t dbSNP:772911765
592 592 c, t dbSNP:771804666
597 597 c, t dbSNP:773022730
606 606 a, g dbSNP:760426076
613 613 a, c dbSNP:147219779
622 622 c, t dbSNP:148601311
623 623 a, g dbSNP:142056890
630 630 a, g dbSNP:764840660
638 638 a, t dbSNP:752051464
639 639 a, g dbSNP:757864779
640 640 -, gct dbSNP:752515915
651 651 c, g, t dbSNP:781658568
654 654 a, g dbSNP:756488352
665 665 a, c, t dbSNP:780310923
666 666 a, g dbSNP:768658357
668 668 c, t dbSNP:778979541
669 669 a, g dbSNP:200478299
679 679 g, t dbSNP:771679154
680 680 a, g dbSNP:763277110
682 682 c, t dbSNP:777055649
688 688 a, g dbSNP:760046572
694 694 a, c, t dbSNP:376763237
695 695 a, g dbSNP:766761476
696 696 a, g dbSNP:186572696
698 698 a, g dbSNP:764203991
720 720 g, t dbSNP:369033190
721 721 a, g dbSNP:761799293
723 723 c, t dbSNP:150731413
724 724 a, g dbSNP:371671456
725 725 c, t dbSNP:756144294
738 738 -, cag dbSNP:774995039
745 745 a, g dbSNP:779955859
746 746 a, g dbSNP:753662640
751 751 g, t dbSNP:201400387
754 754 c, g, t dbSNP:778426094
758 758 c, t dbSNP:771421230
759 759 a, g dbSNP:777282787
761 761 -, gtcc dbSNP:772632958
765 765 c, t dbSNP:553461987
766 766 a, g dbSNP:778304554
768 768 c, g dbSNP:747184229
774 774 c, g dbSNP:200678268
776 776 g, t dbSNP:781315038
780 780 c, t dbSNP:745944280
789 789 a, g dbSNP:769833899
800 800 c, t dbSNP:374327578
802 802 a, c, t dbSNP:200026082
803 803 a, g dbSNP:774163392
810 810 c, t dbSNP:369812524
812 812 a, g dbSNP:143112175
814 814 a, g dbSNP:561690224
823 823 c, t dbSNP:767178490
824 824 a, c, g, t dbSNP:750103889
839 839 a, c dbSNP:753206297
847 847 c, g, t dbSNP:377055075
850 850 c, t dbSNP:751947184
851 851 a, g dbSNP:371452736
854 854 c, t dbSNP:756317638
862 862 c, t dbSNP:375291522
863 863 a, g dbSNP:756322762
865 865 c, g dbSNP:780299105
870 870 c, g, t dbSNP:749352222
893 893 g, t dbSNP:3180160
899 899 a, t dbSNP:139081815
904 904 a, g dbSNP:778820239
907 907 -, c dbSNP:773635676
911 911 c, t dbSNP:144063439
917 917 a, g, t dbSNP:146837754
927 927 c, t dbSNP:140677453
928 928 a, g dbSNP:770684289
935 935 a, t dbSNP:776483823
940 940 a, g dbSNP:759111860
943 943 a, g dbSNP:764842683
944 944 a, g dbSNP:774943231
946 946 c, t dbSNP:762250888
949 949 a, c, g dbSNP:144460879
950 950 c, g dbSNP:367851224
955 955 c, t dbSNP:34745234
959 959 c, t dbSNP:149230396
960 960 a, c, g dbSNP:371556972
964 964 a, g dbSNP:752637049
966 966 c, t dbSNP:758233212
967 967 a, c dbSNP:777814788
970 970 c, t dbSNP:143225348
971 971 a, c, g dbSNP:375834458
980 980 a, g dbSNP:113472219
983 983 a, t dbSNP:201780596
987 987 c, t dbSNP:191438651
988 988 a, g dbSNP:200106123
995 995 c, t dbSNP:753797693
997 997 c, g dbSNP:772440416
1000 1000 c, t dbSNP:773830531
1003 1003 c, t dbSNP:539483296
1004 1004 a, c, t dbSNP:151256721
1005 1005 c, t dbSNP:759782726
1008 1008 c, t dbSNP:200348092
1018 1018 a, c dbSNP:752693628
1023 1023 g, t dbSNP:758563128
1027 1027 c, t dbSNP:777566694
1034 1034 a, g dbSNP:751517268
1045 1045 c, t dbSNP:757059123
1054 1054 a, g dbSNP:201966461
1056 1056 a, c dbSNP:745479177
1071 1071 c, t dbSNP:769541102
1074 1074 a, g dbSNP:779805206
1075 1075 a, g dbSNP:150841343
1076 1076 c, g dbSNP:768282879
1079 1079 a, g dbSNP:773529111
1084 1084 c, t dbSNP:375339833
1085 1085 a, g dbSNP:370506923
1088 1088 c, g dbSNP:182859275
1092 1092 a, g dbSNP:574173340
1101 1101 a, t dbSNP:544769405
1103 1103 a, g dbSNP:563405185
1137 1137 c, t dbSNP:778957473
1186 1186 a, t dbSNP:750265006
1212 1212 a, g dbSNP:530610327
1255 1255 a, g dbSNP:138224344
1260 1260 -, c dbSNP:780494436
1265 1265 a, g dbSNP:576867305
1275 1275 c, g dbSNP:564072310
1309 1309 c, t dbSNP:369583169
1315 1315 a, g dbSNP:574168586
1319 1319 c, g dbSNP:2234315
1330 1330 a, g dbSNP:761187027
1355 1355 a, g dbSNP:771242535
1367 1367 a, g dbSNP:776945507
1378 1378 a, g dbSNP:759938430
1381 1381 c, t dbSNP:765363258
1387 1387 c, t dbSNP:753029496
1388 1388 -, cg dbSNP:761081443
1388 1388 a, c dbSNP:762872607
1389 1389 a, g, t dbSNP:140477675
1390 1390 a, c, t dbSNP:529106769
1391 1391 a, g dbSNP:746937698
1392 1392 a, g dbSNP:528104106
1395 1395 a, g dbSNP:779572025
1396 1396 a, g dbSNP:550737419
1402 1402 c, g, t dbSNP:754391237
1411 1411 c, t dbSNP:569051610
1412 1412 a, g dbSNP:539543556
1415 1415 c, t dbSNP:147063227
1416 1416 a, c, g dbSNP:199740795
1418 1418 g, t dbSNP:775515733
1420 1420 -, tc dbSNP:779859900
1421 1421 c, t dbSNP:369421182
1430 1430 g, t dbSNP:747561622
1431 1431 g, t dbSNP:764042275
1433 1433 c, t dbSNP:774542819
1434 1434 a, g dbSNP:372687754
1445 1445 c, g dbSNP:767549989
1450 1450 c, t dbSNP:376926277
1455 1455 c, t dbSNP:755802675
1456 1456 a, t dbSNP:566536953
1459 1459 a, c dbSNP:766213741
1469 1469 c, t dbSNP:753386633
1472 1472 c, t dbSNP:541210117
1473 1473 a, g dbSNP:2234316
1474 1474 g, t dbSNP:747639120
1476 1476 a, g dbSNP:759992010
1514 1514 a, g dbSNP:555813172
1535 1535 a, g dbSNP:772548532
1543 1543 a, t dbSNP:2651818
1612 1612 c, t dbSNP:574259493
1621 1621 a, g dbSNP:544518851
1693 1693 c, t dbSNP:776203199
1694 1694 c, t dbSNP:188723879

Target ORF information:

RefSeq Version XM_005252719
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005252719

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGCCTA CCTCCTGGGC AGTGAGCTGC GGTCTGAGGC CCCTGCCCAG CTGGAAACCA 
CAGGGAGGGG AAGGGAGGGG AGGAGAGGAG AGGAGAGGAA CCGTCATGGG GCCTTGGAGT
CGAGTCAGGG TTGCCAAATG CCAGATGCTG GTCACCTGCT TCTTTATCTT GCTGCTGGGC
CTCTCTGTGG CCACCATGGT GACTCTTACC TACTTCGGGG CCCACTTTGC TGTCATCCGC
CGAGCGTCCC TGGAGAAGAA CCCGTACCAG GCTGTGCACC AATGGGCCTT CTCTGCGGGG
TTGAGCCTGG TGGGCCTCCT GACTCTGGGA GCCGTGCTGA GCGCTGCAGC CACCGTGAGG
GAGGCCCAGG GCCTCATGGC AGGGGGCTTC CTGTGCTTCT CCCTGGCGTT CTGCGCACAG
GTGCAGGTGG TGTTCTGGAG ACTCCACAGC CCCACCCAGG TGGAGGACGC CATGCTGGAC
ACCTACGACC TGGTATATGA GCAGGCGATG AAAGGTACGT CCCACGTCCG GCGGCAGGAG
CTGGCGGCCA TCCAGGACGT GTTTCTGTGC TGTGGGAAGA AGTCTCCTTT CAGCCGTCTG
GGGAGCACAG AGGCTGACCT GTGTCAGGGA GAGGAGGCGG CGAGAGAGGA CTGCCTTCAG
GGCATCCGGA GCTTCCTGAG GACACACCAG CAGGTCGCCT CCAGCCTGAC CAGCATCGGC
CTGGCCCTCA CGGTGTCCGC CTTGCTCTTC AGCTCCTTCC TGTGGTTTGC CATCCGCTGT
GGCTGCAGCT TGGACCGCAA GGGCAAATAC ACCCTGACCC CACGAGCATG TGGCCGCCAG
CCCCAGGAGC CCAGCCTCTT GAGATGCTCC CAGGGTGGAC CCACACATTG TCTCCACTCC
GAAGCAGTTG CTATTGGTCC AAGAGGATGC TCGGGTAGTC TTCGGTGGCT GCAGGAGAGC
GATGCTGCGC CTCTGCCCCT CTCCTGCCAC CTGGCTGCCC ACAGAGGTGA AGACGCCCCT
GCTGTCAGCC CTCATGGGAT CCCTGAGGGG AGGGTCCGAG CTGTGAGGAG GGAAGGGAGT
GAAGGCCCAG CCAGAGAGCC AGGCTCCATT GGGAACAGAT GCAAGGGTAA GGGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63665
Accession Version XM_011519812.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1029bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tetraspanin-32 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGGCCTT GGAGTCGAGT CAGGGTTGCC AAATGCCAGA TGCTGGTCAC CTGCTTCTTT 
ATCTTGCTGC TGGGCCTCTC TGTGGCCACC ATGGTGACTC TTACCTACTT CGGGGCCCAC
TTTGCTGTCA TCCGCCGAGC GTCCCTGGAG AAGAACCCGT ACCAGGCTGT GCACCAATGG
GGGACTCAGC AGCGACTTAT CCAACATCCA GAGAGCGGGA GCGAGGGCCA GAGCCTGCTG
GGGCCACTCA GGGCCTTCTC TGCGGGGTTG AGCCTGGTGG GCCTCCTGAC TCTGGGAGCC
GTGCTGAGCG CTGCAGCCAC CGTGAGGGAG GCCCAGGGCC TCATGGCAGG GGTGGAGGAC
GCCATGCTGG ACACCTACGA CCTGGTATAT GAGCAGGCGA TGAAAGGTAC GTCCCACGTC
CGGCGGCAGG AGCTGGCGGC CATCCAGGAC GTGTTTCTGT GCTGTGGGAA GAAGTCTCCT
TTCAGCCGTC TGGGGAGCAC AGAGGCTGAC CTGTGTCAGG GAGAGGAGGC GGCGAGAGAG
GACTGCCTTC AGGGCATCCG GAGCTTCCTG AGGACACACC AGCAGGTCGC CTCCAGCCTG
ACCAGCATCG GCCTGGCCCT CACGGTGTCC GCCTTGCTCT TCAGCTCCTT CCTGTGGTTT
GCCATCCGCT GTGGCTGCAG CTTGGACCGC AAGGGCAAAT ACACCCTGAC CCCACGAGCA
TGTGGCCGCC AGCCCCAGGA GCCCAGCCTC TTGAGATGCT CCCAGGGTGG ACCCACACAT
TGTCTCCACT CCGAAGCAGT TGCTATTGGT CCAAGAGGAT GCTCGGGTAG TCTTCGGTGG
CTGCAGGAGA GCGATGCTGC GCCTCTGCCC CTCTCCTGCC ACCTGGCTGC CCACAGAGGT
GAAGACGCCC CTGCTGTCAG CCCTCATGGG ATCCCTGAGG GGAGGGTCCG AGCTGTGAGG
AGGGAAGGGA GTGAAGGCCC AGCCAGAGAG CCAGGCTCCA TTGGGAACAG ATGCAAGGGT
AAGGGGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011518114.1
CDS18..1046
Misc Feature(1)366..599(+)
Misc Feature(2)369..473(+)
Translation
Position Chain Variation Link
11 11 a, g dbSNP:756986960
13 13 a, c dbSNP:781030842
14 14 c, t dbSNP:750010162
15 15 a, g dbSNP:532243385
24 24 a, c dbSNP:149680436
27 27 c, t dbSNP:748716777
33 33 c, t dbSNP:145517785
34 34 a, g dbSNP:148861275
34 34 -, g dbSNP:776304562
42 42 g, t dbSNP:778131898
53 53 a, c dbSNP:747621957
56 56 c, g dbSNP:142403358
61 61 a, c, t dbSNP:777152824
62 62 a, g dbSNP:770167215
75 75 g, t dbSNP:775892076
83 83 a, g dbSNP:762917262
84 84 a, c dbSNP:200210112
91 91 a, g dbSNP:758018002
98 98 g, t dbSNP:543107439
100 100 c, t dbSNP:746432153
105 105 a, t dbSNP:756540607
111 111 g, t dbSNP:780470109
115 115 c, t dbSNP:749412499
122 122 c, t dbSNP:768944967
128 128 c, t dbSNP:146480945
129 129 -, g dbSNP:750786852
133 133 c, t dbSNP:369696526
140 140 c, g, t dbSNP:576767301
148 148 c, t dbSNP:540982577
150 150 a, c, t dbSNP:140709712
151 151 a, g dbSNP:201106606
153 153 a, c, t dbSNP:559404880
154 154 a, g, t dbSNP:371441475
157 157 c, t dbSNP:529974468
158 158 a, g dbSNP:368347803
162 162 c, g dbSNP:762542013
175 175 c, t dbSNP:377338457
179 179 c, t dbSNP:750896014
180 180 a, c dbSNP:756704119
183 183 c, g dbSNP:145905096
184 184 a, c dbSNP:770456957
191 191 a, c dbSNP:755347022
193 193 a, c dbSNP:181067559
194 194 a, g dbSNP:531066922
198 198 a, g dbSNP:748434290
238 238 g, t dbSNP:371482652
251 251 c, t dbSNP:1501467
271 271 c, g dbSNP:774188366
279 279 a, g dbSNP:761371873
281 281 a, g dbSNP:201530910
283 283 a, g dbSNP:777126996
291 291 c, t dbSNP:759972432
292 292 c, t dbSNP:376702018
293 293 c, g dbSNP:765534620
301 301 c, t dbSNP:753089277
304 304 c, t dbSNP:138170136
308 308 g, t dbSNP:555848083
311 311 a, g dbSNP:371071821
312 312 a, g dbSNP:200631781
313 313 c, g dbSNP:751841969
318 318 a, g dbSNP:2234296
326 326 a, c, t dbSNP:199650987
327 327 a, c, g dbSNP:201634419
330 330 g, t dbSNP:749060868
339 339 a, g dbSNP:545681469
344 344 a, g dbSNP:774141716
345 345 a, g dbSNP:747759649
348 348 g, t dbSNP:200624662
356 356 c, t dbSNP:772617887
359 359 c, t dbSNP:760045340
361 361 c, t dbSNP:61744929
365 365 a, g dbSNP:776021550
368 368 a, g dbSNP:763294199
372 372 c, g dbSNP:775228702
374 374 a, g dbSNP:367966944
377 377 c, t dbSNP:2234300
378 378 a, g dbSNP:2234301
390 390 -, acctacg dbSNP:779134770
391 391 c, g dbSNP:757085417
394 394 a, g dbSNP:767136719
395 395 c, t dbSNP:147630481
399 399 a, c dbSNP:755576262
401 401 a, g dbSNP:779628656
410 410 a, g dbSNP:748555005
415 415 c, t dbSNP:758973942
416 416 a, g dbSNP:778462230
424 424 g, t dbSNP:112455917
427 427 c, t dbSNP:554395460
434 434 c, t dbSNP:368872715
438 438 c, t dbSNP:374473348
439 439 a, g dbSNP:138129469
442 442 a, g dbSNP:2234302
454 454 c, t dbSNP:561781505
455 455 a, g dbSNP:764063848
467 467 c, t dbSNP:541981635
468 468 a, g dbSNP:759043414
471 471 c, t dbSNP:772911765
474 474 c, t dbSNP:771804666
479 479 c, t dbSNP:773022730
488 488 a, g dbSNP:760426076
495 495 a, c dbSNP:147219779
504 504 c, t dbSNP:148601311
505 505 a, g dbSNP:142056890
512 512 a, g dbSNP:764840660
520 520 a, t dbSNP:752051464
521 521 a, g dbSNP:757864779
522 522 -, gct dbSNP:752515915
533 533 c, g, t dbSNP:781658568
536 536 a, g dbSNP:756488352
547 547 a, c, t dbSNP:780310923
548 548 a, g dbSNP:768658357
550 550 c, t dbSNP:778979541
551 551 a, g dbSNP:200478299
561 561 g, t dbSNP:771679154
562 562 a, g dbSNP:763277110
564 564 c, t dbSNP:777055649
570 570 a, g dbSNP:760046572
576 576 a, c, t dbSNP:376763237
577 577 a, g dbSNP:766761476
578 578 a, g dbSNP:186572696
580 580 a, g dbSNP:764203991
602 602 g, t dbSNP:369033190
603 603 a, g dbSNP:761799293
605 605 c, t dbSNP:150731413
606 606 a, g dbSNP:371671456
607 607 c, t dbSNP:756144294
620 620 -, cag dbSNP:774995039
627 627 a, g dbSNP:779955859
628 628 a, g dbSNP:753662640
633 633 g, t dbSNP:201400387
636 636 c, g, t dbSNP:778426094
640 640 c, t dbSNP:771421230
641 641 a, g dbSNP:777282787
643 643 -, gtcc dbSNP:772632958
647 647 c, t dbSNP:553461987
648 648 a, g dbSNP:778304554
650 650 c, g dbSNP:747184229
656 656 c, g dbSNP:200678268
658 658 g, t dbSNP:781315038
662 662 c, t dbSNP:745944280
671 671 a, g dbSNP:769833899
682 682 c, t dbSNP:374327578
684 684 a, c, t dbSNP:200026082
685 685 a, g dbSNP:774163392
692 692 c, t dbSNP:369812524
694 694 a, g dbSNP:143112175
696 696 a, g dbSNP:561690224
705 705 c, t dbSNP:767178490
706 706 a, c, g, t dbSNP:750103889
721 721 a, c dbSNP:753206297
729 729 c, g, t dbSNP:377055075
732 732 c, t dbSNP:751947184
733 733 a, g dbSNP:371452736
736 736 c, t dbSNP:756317638
744 744 c, t dbSNP:375291522
745 745 a, g dbSNP:756322762
747 747 c, g dbSNP:780299105
752 752 c, g, t dbSNP:749352222
775 775 g, t dbSNP:3180160
781 781 a, t dbSNP:139081815
786 786 a, g dbSNP:778820239
789 789 -, c dbSNP:773635676
793 793 c, t dbSNP:144063439
799 799 a, g, t dbSNP:146837754
809 809 c, t dbSNP:140677453
810 810 a, g dbSNP:770684289
817 817 a, t dbSNP:776483823
822 822 a, g dbSNP:759111860
825 825 a, g dbSNP:764842683
826 826 a, g dbSNP:774943231
828 828 c, t dbSNP:762250888
831 831 a, c, g dbSNP:144460879
832 832 c, g dbSNP:367851224
837 837 c, t dbSNP:34745234
841 841 c, t dbSNP:149230396
842 842 a, c, g dbSNP:371556972
846 846 a, g dbSNP:752637049
848 848 c, t dbSNP:758233212
849 849 a, c dbSNP:777814788
852 852 c, t dbSNP:143225348
853 853 a, c, g dbSNP:375834458
862 862 a, g dbSNP:113472219
865 865 a, t dbSNP:201780596
869 869 c, t dbSNP:191438651
870 870 a, g dbSNP:200106123
877 877 c, t dbSNP:753797693
879 879 c, g dbSNP:772440416
882 882 c, t dbSNP:773830531
885 885 c, t dbSNP:539483296
886 886 a, c, t dbSNP:151256721
887 887 c, t dbSNP:759782726
890 890 c, t dbSNP:200348092
900 900 a, c dbSNP:752693628
905 905 g, t dbSNP:758563128
909 909 c, t dbSNP:777566694
916 916 a, g dbSNP:751517268
927 927 c, t dbSNP:757059123
936 936 a, g dbSNP:201966461
938 938 a, c dbSNP:745479177
953 953 c, t dbSNP:769541102
956 956 a, g dbSNP:779805206
957 957 a, g dbSNP:150841343
958 958 c, g dbSNP:768282879
961 961 a, g dbSNP:773529111
966 966 c, t dbSNP:375339833
967 967 a, g dbSNP:370506923
970 970 c, g dbSNP:182859275
974 974 a, g dbSNP:574173340
983 983 a, t dbSNP:544769405
985 985 a, g dbSNP:563405185
1019 1019 c, t dbSNP:778957473
1068 1068 a, t dbSNP:750265006
1094 1094 a, g dbSNP:530610327
1137 1137 a, g dbSNP:138224344
1142 1142 -, c dbSNP:780494436
1147 1147 a, g dbSNP:576867305
1157 1157 c, g dbSNP:564072310
1191 1191 c, t dbSNP:369583169
1197 1197 a, g dbSNP:574168586
1201 1201 c, g dbSNP:2234315
1212 1212 a, g dbSNP:761187027
1237 1237 a, g dbSNP:771242535
1249 1249 a, g dbSNP:776945507
1260 1260 a, g dbSNP:759938430
1263 1263 c, t dbSNP:765363258
1269 1269 c, t dbSNP:753029496
1270 1270 -, cg dbSNP:761081443
1270 1270 a, c dbSNP:762872607
1271 1271 a, g, t dbSNP:140477675
1272 1272 a, c, t dbSNP:529106769
1273 1273 a, g dbSNP:746937698
1274 1274 a, g dbSNP:528104106
1277 1277 a, g dbSNP:779572025
1278 1278 a, g dbSNP:550737419
1284 1284 c, g, t dbSNP:754391237
1293 1293 c, t dbSNP:569051610
1294 1294 a, g dbSNP:539543556
1297 1297 c, t dbSNP:147063227
1298 1298 a, c, g dbSNP:199740795
1300 1300 g, t dbSNP:775515733
1302 1302 -, tc dbSNP:779859900
1303 1303 c, t dbSNP:369421182
1312 1312 g, t dbSNP:747561622
1313 1313 g, t dbSNP:764042275
1315 1315 c, t dbSNP:774542819
1316 1316 a, g dbSNP:372687754
1327 1327 c, g dbSNP:767549989
1332 1332 c, t dbSNP:376926277
1337 1337 c, t dbSNP:755802675
1338 1338 a, t dbSNP:566536953
1341 1341 a, c dbSNP:766213741
1351 1351 c, t dbSNP:753386633
1354 1354 c, t dbSNP:541210117
1355 1355 a, g dbSNP:2234316
1356 1356 g, t dbSNP:747639120
1358 1358 a, g dbSNP:759992010
1396 1396 a, g dbSNP:555813172
1417 1417 a, g dbSNP:772548532
1425 1425 a, t dbSNP:2651818
1494 1494 c, t dbSNP:574259493
1503 1503 a, g dbSNP:544518851
1575 1575 c, t dbSNP:776203199
1576 1576 c, t dbSNP:188723879

Target ORF information:

RefSeq Version XM_011519812
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519812

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGGCCTT GGAGTCGAGT CAGGGTTGCC AAATGCCAGA TGCTGGTCAC CTGCTTCTTT 
ATCTTGCTGC TGGGCCTCTC TGTGGCCACC ATGGTGACTC TTACCTACTT CGGGGCCCAC
TTTGCTGTCA TCCGCCGAGC GTCCCTGGAG AAGAACCCGT ACCAGGCTGT GCACCAATGG
GGGACTCAGC AGCGACTTAT CCAACATCCA GAGAGCGGGA GCGAGGGCCA GAGCCTGCTG
GGGCCACTCA GGGCCTTCTC TGCGGGGTTG AGCCTGGTGG GCCTCCTGAC TCTGGGAGCC
GTGCTGAGCG CTGCAGCCAC CGTGAGGGAG GCCCAGGGCC TCATGGCAGG GGTGGAGGAC
GCCATGCTGG ACACCTACGA CCTGGTATAT GAGCAGGCGA TGAAAGGTAC GTCCCACGTC
CGGCGGCAGG AGCTGGCGGC CATCCAGGAC GTGTTTCTGT GCTGTGGGAA GAAGTCTCCT
TTCAGCCGTC TGGGGAGCAC AGAGGCTGAC CTGTGTCAGG GAGAGGAGGC GGCGAGAGAG
GACTGCCTTC AGGGCATCCG GAGCTTCCTG AGGACACACC AGCAGGTCGC CTCCAGCCTG
ACCAGCATCG GCCTGGCCCT CACGGTGTCC GCCTTGCTCT TCAGCTCCTT CCTGTGGTTT
GCCATCCGCT GTGGCTGCAG CTTGGACCGC AAGGGCAAAT ACACCCTGAC CCCACGAGCA
TGTGGCCGCC AGCCCCAGGA GCCCAGCCTC TTGAGATGCT CCCAGGGTGG ACCCACACAT
TGTCTCCACT CCGAAGCAGT TGCTATTGGT CCAAGAGGAT GCTCGGGTAG TCTTCGGTGG
CTGCAGGAGA GCGATGCTGC GCCTCTGCCC CTCTCCTGCC ACCTGGCTGC CCACAGAGGT
GAAGACGCCC CTGCTGTCAG CCCTCATGGG ATCCCTGAGG GGAGGGTCCG AGCTGTGAGG
AGGGAAGGGA GTGAAGGCCC AGCCAGAGAG CCAGGCTCCA TTGGGAACAG ATGCAAGGGT
AAGGGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63666
Accession Version XM_011519813.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1125bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tetraspanin-32 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGCCTA CCTCCTGGGC AGTGAGCTGC GGTCTGAGGC CCCTGCCCAG CTGGAAACCA 
CAGGGAGGGG AAGGGAGGGG AGGAGAGGAG AGGAGAGGAA CCGTCATGGG GCCTTGGAGT
CGAGTCAGGG TTGCCAAATG CCAGATGCTG GTCACCTGCT TCTTTATCTT GCTGCTGGGC
CTCTCTGTGG CCACCATGGT GACTCTTACC TACTTCGGGG CCCACTTTGC TGTCATCCGC
CGAGCGTCCC TGGAGAAGAA CCCGTACCAG GCTGTGCACC AATGGGGGAC TCAGCAGCGA
CTTATCCAAC ATCCAGAGAG CGGGAGCGAG GGCCAGAGCC TGCTGGGGCC ACTCAGGGCC
TTCTCTGCGG GGTTGAGCCT GGTGGGCCTC CTGACTCTGG GAGCCGTGCT GAGCGCTGCA
GCCACCGTGA GGGAGGCCCA GGGCCTCATG GCAGGGGGCT TCCTGTGCTT CTCCCTGGCG
TTCTGCGCAC AGGTGCAGGT GGTGTTCTGG AGACTCCACA GCCCCACCCA GGTGGAGGAC
GCCATGCTGG ACACCTACGA CCTGGTATAT GAGCAGGCGA TGAAAGGTAC GTCCCACGTC
CGGCGGCAGG AGCTGGCGGC CATCCAGGAC GTGTTTCTGT GCTGTGGGAA GAAGTCTCCT
TTCAGCCGTC TGGGGAGCAC AGAGGCTGAC CTGTGTCAGG GAGAGGAGGC GGCGAGAGAG
GTGTCCGCCT TGCTCTTCAG CTCCTTCCTG TGGTTTGCCA TCCGCTGTGG CTGCAGCTTG
GACCGCAAGG GCAAATACAC CCTGACCCCA CGAGCATGTG GCCGCCAGCC CCAGGAGCCC
AGCCTCTTGA GATGCTCCCA GGGTGGACCC ACACATTGTC TCCACTCCGA AGCAGTTGCT
ATTGGTCCAA GAGGATGCTC GGGTAGTCTT CGGTGGCTGC AGGAGAGCGA TGCTGCGCCT
CTGCCCCTCT CCTGCCACCT GGCTGCCCAC AGAGGTGAAG ACGCCCCTGC TGTCAGCCCT
CATGGGATCC CTGAGGGGAG GGTCCGAGCT GTGAGGAGGG AAGGGAGTGA AGGCCCAGCC
AGAGAGCCAG GCTCCATTGG GAACAGATGC AAGGGTAAGG GGTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011518115.1
CDS33..1157
Misc Feature(1)168..>728(+)
Misc Feature(2)546..809(+)
Misc Feature(3)549..668(+)
Translation
Position Chain Variation Link
2 2 -, ggg dbSNP:777634526
4 4 a, g dbSNP:371953629
5 5 c, t dbSNP:757112917
6 6 a, c, g dbSNP:780797896
7 7 a, t dbSNP:769288063
10 10 c, t dbSNP:779543727
30 30 c, t dbSNP:748595793
31 31 a, g dbSNP:772487085
34 34 g, t dbSNP:773810149
40 40 c, t dbSNP:760927991
49 49 g, t dbSNP:375443053
62 62 c, g, t dbSNP:78465685
63 63 a, g dbSNP:564849421
70 70 a, g dbSNP:765354088
78 78 a, c dbSNP:372594309
82 82 a, g dbSNP:762904620
95 95 g, t dbSNP:763904934
107 107 a, g dbSNP:375516709
111 111 -, gagga dbSNP:746834021
112 112 -, gagga dbSNP:770612134
131 131 a, g dbSNP:756986960
133 133 a, c dbSNP:781030842
134 134 c, t dbSNP:750010162
135 135 a, g dbSNP:532243385
144 144 a, c dbSNP:149680436
147 147 c, t dbSNP:748716777
153 153 c, t dbSNP:145517785
154 154 a, g dbSNP:148861275
154 154 -, g dbSNP:776304562
162 162 g, t dbSNP:778131898
173 173 a, c dbSNP:747621957
176 176 c, g dbSNP:142403358
181 181 a, c, t dbSNP:777152824
182 182 a, g dbSNP:770167215
195 195 g, t dbSNP:775892076
203 203 a, g dbSNP:762917262
204 204 a, c dbSNP:200210112
211 211 a, g dbSNP:758018002
218 218 g, t dbSNP:543107439
220 220 c, t dbSNP:746432153
225 225 a, t dbSNP:756540607
231 231 g, t dbSNP:780470109
235 235 c, t dbSNP:749412499
242 242 c, t dbSNP:768944967
248 248 c, t dbSNP:146480945
249 249 -, g dbSNP:750786852
253 253 c, t dbSNP:369696526
260 260 c, g, t dbSNP:576767301
268 268 c, t dbSNP:540982577
270 270 a, c, t dbSNP:140709712
271 271 a, g dbSNP:201106606
273 273 a, c, t dbSNP:559404880
274 274 a, g, t dbSNP:371441475
277 277 c, t dbSNP:529974468
278 278 a, g dbSNP:368347803
282 282 c, g dbSNP:762542013
295 295 c, t dbSNP:377338457
299 299 c, t dbSNP:750896014
300 300 a, c dbSNP:756704119
303 303 c, g dbSNP:145905096
304 304 a, c dbSNP:770456957
311 311 a, c dbSNP:755347022
313 313 a, c dbSNP:181067559
314 314 a, g dbSNP:531066922
318 318 a, g dbSNP:748434290
358 358 g, t dbSNP:371482652
371 371 c, t dbSNP:1501467
391 391 c, g dbSNP:774188366
399 399 a, g dbSNP:761371873
401 401 a, g dbSNP:201530910
403 403 a, g dbSNP:777126996
411 411 c, t dbSNP:759972432
412 412 c, t dbSNP:376702018
413 413 c, g dbSNP:765534620
421 421 c, t dbSNP:753089277
424 424 c, t dbSNP:138170136
428 428 g, t dbSNP:555848083
431 431 a, g dbSNP:371071821
432 432 a, g dbSNP:200631781
433 433 c, g dbSNP:751841969
438 438 a, g dbSNP:2234296
446 446 a, c, t dbSNP:199650987
447 447 a, c, g dbSNP:201634419
450 450 g, t dbSNP:749060868
459 459 a, g dbSNP:545681469
464 464 a, g dbSNP:774141716
465 465 a, g dbSNP:747759649
468 468 g, t dbSNP:200624662
476 476 c, t dbSNP:772617887
479 479 c, t dbSNP:760045340
481 481 c, t dbSNP:61744929
485 485 a, g dbSNP:776021550
488 488 a, g dbSNP:763294199
495 495 a, c dbSNP:768954809
506 506 c, t dbSNP:774603549
507 507 a, c dbSNP:372246596
511 511 c, t dbSNP:767604508
512 512 a, g dbSNP:570557887
518 518 a, c, g, t dbSNP:11554947
519 519 a, g dbSNP:770634605
525 525 g, t dbSNP:753770845
528 528 c, t dbSNP:754985828
539 539 a, c dbSNP:778727393
542 542 a, g dbSNP:752399702
543 543 a, g dbSNP:758260127
558 558 a, g dbSNP:376562078
567 567 c, g dbSNP:775228702
569 569 a, g dbSNP:367966944
572 572 c, t dbSNP:2234300
573 573 a, g dbSNP:2234301
585 585 -, acctacg dbSNP:779134770
586 586 c, g dbSNP:757085417
589 589 a, g dbSNP:767136719
590 590 c, t dbSNP:147630481
594 594 a, c dbSNP:755576262
596 596 a, g dbSNP:779628656
605 605 a, g dbSNP:748555005
610 610 c, t dbSNP:758973942
611 611 a, g dbSNP:778462230
619 619 g, t dbSNP:112455917
622 622 c, t dbSNP:554395460
629 629 c, t dbSNP:368872715
633 633 c, t dbSNP:374473348
634 634 a, g dbSNP:138129469
637 637 a, g dbSNP:2234302
649 649 c, t dbSNP:561781505
650 650 a, g dbSNP:764063848
662 662 c, t dbSNP:541981635
663 663 a, g dbSNP:759043414
666 666 c, t dbSNP:772911765
669 669 c, t dbSNP:771804666
674 674 c, t dbSNP:773022730
683 683 a, g dbSNP:760426076
690 690 a, c dbSNP:147219779
699 699 c, t dbSNP:148601311
700 700 a, g dbSNP:142056890
707 707 a, g dbSNP:764840660
715 715 a, t dbSNP:752051464
716 716 a, g dbSNP:757864779
717 717 -, gct dbSNP:752515915
728 728 c, g, t dbSNP:781658568
731 731 a, g dbSNP:756488352
742 742 a, c, t dbSNP:780310923
743 743 a, g dbSNP:768658357
745 745 c, t dbSNP:778979541
746 746 a, g dbSNP:200478299
754 754 -, gtcc dbSNP:772632958
758 758 c, t dbSNP:553461987
759 759 a, g dbSNP:778304554
761 761 c, g dbSNP:747184229
767 767 c, g dbSNP:200678268
769 769 g, t dbSNP:781315038
773 773 c, t dbSNP:745944280
782 782 a, g dbSNP:769833899
793 793 c, t dbSNP:374327578
795 795 a, c, t dbSNP:200026082
796 796 a, g dbSNP:774163392
803 803 c, t dbSNP:369812524
805 805 a, g dbSNP:143112175
807 807 a, g dbSNP:561690224
816 816 c, t dbSNP:767178490
817 817 a, c, g, t dbSNP:750103889
832 832 a, c dbSNP:753206297
840 840 c, g, t dbSNP:377055075
843 843 c, t dbSNP:751947184
844 844 a, g dbSNP:371452736
847 847 c, t dbSNP:756317638
855 855 c, t dbSNP:375291522
856 856 a, g dbSNP:756322762
858 858 c, g dbSNP:780299105
863 863 c, g, t dbSNP:749352222
886 886 g, t dbSNP:3180160
892 892 a, t dbSNP:139081815
897 897 a, g dbSNP:778820239
900 900 -, c dbSNP:773635676
904 904 c, t dbSNP:144063439
910 910 a, g, t dbSNP:146837754
920 920 c, t dbSNP:140677453
921 921 a, g dbSNP:770684289
928 928 a, t dbSNP:776483823
933 933 a, g dbSNP:759111860
936 936 a, g dbSNP:764842683
937 937 a, g dbSNP:774943231
939 939 c, t dbSNP:762250888
942 942 a, c, g dbSNP:144460879
943 943 c, g dbSNP:367851224
948 948 c, t dbSNP:34745234
952 952 c, t dbSNP:149230396
953 953 a, c, g dbSNP:371556972
957 957 a, g dbSNP:752637049
959 959 c, t dbSNP:758233212
960 960 a, c dbSNP:777814788
963 963 c, t dbSNP:143225348
964 964 a, c, g dbSNP:375834458
973 973 a, g dbSNP:113472219
976 976 a, t dbSNP:201780596
980 980 c, t dbSNP:191438651
981 981 a, g dbSNP:200106123
988 988 c, t dbSNP:753797693
990 990 c, g dbSNP:772440416
993 993 c, t dbSNP:773830531
996 996 c, t dbSNP:539483296
997 997 a, c, t dbSNP:151256721
998 998 c, t dbSNP:759782726
1001 1001 c, t dbSNP:200348092
1011 1011 a, c dbSNP:752693628
1016 1016 g, t dbSNP:758563128
1020 1020 c, t dbSNP:777566694
1027 1027 a, g dbSNP:751517268
1038 1038 c, t dbSNP:757059123
1047 1047 a, g dbSNP:201966461
1049 1049 a, c dbSNP:745479177
1064 1064 c, t dbSNP:769541102
1067 1067 a, g dbSNP:779805206
1068 1068 a, g dbSNP:150841343
1069 1069 c, g dbSNP:768282879
1072 1072 a, g dbSNP:773529111
1077 1077 c, t dbSNP:375339833
1078 1078 a, g dbSNP:370506923
1081 1081 c, g dbSNP:182859275
1085 1085 a, g dbSNP:574173340
1094 1094 a, t dbSNP:544769405
1096 1096 a, g dbSNP:563405185
1130 1130 c, t dbSNP:778957473
1179 1179 a, t dbSNP:750265006
1205 1205 a, g dbSNP:530610327
1248 1248 a, g dbSNP:138224344
1253 1253 -, c dbSNP:780494436
1258 1258 a, g dbSNP:576867305
1268 1268 c, g dbSNP:564072310
1302 1302 c, t dbSNP:369583169
1308 1308 a, g dbSNP:574168586
1312 1312 c, g dbSNP:2234315
1323 1323 a, g dbSNP:761187027
1348 1348 a, g dbSNP:771242535
1360 1360 a, g dbSNP:776945507
1371 1371 a, g dbSNP:759938430
1374 1374 c, t dbSNP:765363258
1380 1380 c, t dbSNP:753029496
1381 1381 -, cg dbSNP:761081443
1381 1381 a, c dbSNP:762872607
1382 1382 a, g, t dbSNP:140477675
1383 1383 a, c, t dbSNP:529106769
1384 1384 a, g dbSNP:746937698
1385 1385 a, g dbSNP:528104106
1388 1388 a, g dbSNP:779572025
1389 1389 a, g dbSNP:550737419
1395 1395 c, g, t dbSNP:754391237
1404 1404 c, t dbSNP:569051610
1405 1405 a, g dbSNP:539543556
1408 1408 c, t dbSNP:147063227
1409 1409 a, c, g dbSNP:199740795
1411 1411 g, t dbSNP:775515733
1413 1413 -, tc dbSNP:779859900
1414 1414 c, t dbSNP:369421182
1423 1423 g, t dbSNP:747561622
1424 1424 g, t dbSNP:764042275
1426 1426 c, t dbSNP:774542819
1427 1427 a, g dbSNP:372687754
1438 1438 c, g dbSNP:767549989
1443 1443 c, t dbSNP:376926277
1448 1448 c, t dbSNP:755802675
1449 1449 a, t dbSNP:566536953
1452 1452 a, c dbSNP:766213741
1462 1462 c, t dbSNP:753386633
1465 1465 c, t dbSNP:541210117
1466 1466 a, g dbSNP:2234316
1467 1467 g, t dbSNP:747639120
1469 1469 a, g dbSNP:759992010
1507 1507 a, g dbSNP:555813172
1528 1528 a, g dbSNP:772548532
1536 1536 a, t dbSNP:2651818
1605 1605 c, t dbSNP:574259493
1614 1614 a, g dbSNP:544518851
1686 1686 c, t dbSNP:776203199
1687 1687 c, t dbSNP:188723879

Target ORF information:

RefSeq Version XM_011519813
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens tetraspanin 32 (TSPAN32), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519813

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGCCTA CCTCCTGGGC AGTGAGCTGC GGTCTGAGGC CCCTGCCCAG CTGGAAACCA 
CAGGGAGGGG AAGGGAGGGG AGGAGAGGAG AGGAGAGGAA CCGTCATGGG GCCTTGGAGT
CGAGTCAGGG TTGCCAAATG CCAGATGCTG GTCACCTGCT TCTTTATCTT GCTGCTGGGC
CTCTCTGTGG CCACCATGGT GACTCTTACC TACTTCGGGG CCCACTTTGC TGTCATCCGC
CGAGCGTCCC TGGAGAAGAA CCCGTACCAG GCTGTGCACC AATGGGGGAC TCAGCAGCGA
CTTATCCAAC ATCCAGAGAG CGGGAGCGAG GGCCAGAGCC TGCTGGGGCC ACTCAGGGCC
TTCTCTGCGG GGTTGAGCCT GGTGGGCCTC CTGACTCTGG GAGCCGTGCT GAGCGCTGCA
GCCACCGTGA GGGAGGCCCA GGGCCTCATG GCAGGGGGCT TCCTGTGCTT CTCCCTGGCG
TTCTGCGCAC AGGTGCAGGT GGTGTTCTGG AGACTCCACA GCCCCACCCA GGTGGAGGAC
GCCATGCTGG ACACCTACGA CCTGGTATAT GAGCAGGCGA TGAAAGGTAC GTCCCACGTC
CGGCGGCAGG AGCTGGCGGC CATCCAGGAC GTGTTTCTGT GCTGTGGGAA GAAGTCTCCT
TTCAGCCGTC TGGGGAGCAC AGAGGCTGAC CTGTGTCAGG GAGAGGAGGC GGCGAGAGAG
GTGTCCGCCT TGCTCTTCAG CTCCTTCCTG TGGTTTGCCA TCCGCTGTGG CTGCAGCTTG
GACCGCAAGG GCAAATACAC CCTGACCCCA CGAGCATGTG GCCGCCAGCC CCAGGAGCCC
AGCCTCTTGA GATGCTCCCA GGGTGGACCC ACACATTGTC TCCACTCCGA AGCAGTTGCT
ATTGGTCCAA GAGGATGCTC GGGTAGTCTT CGGTGGCTGC AGGAGAGCGA TGCTGCGCCT
CTGCCCCTCT CCTGCCACCT GGCTGCCCAC AGAGGTGAAG ACGCCCCTGC TGTCAGCCCT
CATGGGATCC CTGAGGGGAG GGTCCGAGCT GTGAGGAGGG AAGGGAGTGA AGGCCCAGCC
AGAGAGCCAG GCTCCATTGG GAACAGATGC AAGGGTAAGG GGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63667
Accession Version XM_011519814.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tetraspanin-32 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_009237.19) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCAGATG CTGGTCACCT GCTTCTTTAT CTTGGTAACA GGCAGCTGCT GGGCCTCTCT 
GTGGCCACCA TGGTGACTCT TACCTACTTC GGGGCCCACT TTGCTGTCAT CCGCCGAGCG
TCCCTGGAGA AGAACCCGTA CCAGGCTGTG CACCAATGGG GGACTCAGCA GCGACTTATC
CAACATCCAG AGAGCGGGAG CGAGGGCCAG AGCCTGCTGG GGCCACTCAG GGCCTTCTCT
GCGGGGTTGA GCCTGGTGGG CCTCCTGACT CTGGGAGCCG TGCTGAGCGC TGCAGCCACC
GTGAGGGAGG CCCAGGGCCT CATGGCAGGG GGCTTCCTGT GCTTCTCCCT GGCGTTCTGC
GCACAGGTGC AGGTGGTGTT CTGGAGACTC CACAGCCCCA CCCAGGTGGA GGACGCCATG
CTGGACACCT ACGACCTGGT ATATGAGCAG GCGATGAAAG GTACGTCCCA CGTCCGGCGG
CAGGAGCTGG CGGCCATCCA GGACGTGTTT CTGTGCTGTG GGAAGAAGTC TCCTTTCAGC
CGTCTGGGGA GCACAGAGGC TGACCTGTGT CAGGGAGAGG AGGCGGCGAG AGAGGACTGC
CTTCAGGGCA TCCGGAGCTT CCTGAGGACA CACCAGCAGG TCGCCTCCAG CCTGACCAGC
ATCGGCCTGG CCCTCACGGT GTCCGCCTTG CTCTTCAGCT CCTTCCTGTG GTTTGCCATC
CGCTGTGGCT GCAGCTTGGA CCGCAAGGGC AAATACACCC TGACCCCACG AGCATGTGGC
CGCCAGCCCC AGGAGCCCAG CCTCTTGAGA TGCTCCCAGG GTGGACCCAC ACATTGTCTC
CACTCCGAAG CAGTTGCTAT TGGTCCAAGA GGATGCTCGG GTAGTCTTCG GTGGCTGCAG
GAGAGCGATG CTGCGCCTCT GCCCCTCTCC TGCCACCTGG CTGCCCACAG AGGTGAAGAC
GCCCCTGCTG TCAGCCCTCA TGGGATCCCT GAGGGGAGGG TCCGAGCTGT GAGGAGGGAA
GGGAGTGAAG GCCCAGCCAG AGAGCCAGGC TCCATTGGGA ACAGATGCAA GGGTAAGGGG
TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011518116.1
CDS37..1119
Misc Feature(1)247..756(+)
Misc Feature(2)424..672(+)
Misc Feature(3)427..546(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:750010162
2 2 a, g dbSNP:532243385
11 11 a, c dbSNP:149680436
14 14 c, t dbSNP:748716777
20 20 c, t dbSNP:145517785
21 21 a, g dbSNP:148861275
21 21 -, g dbSNP:776304562
29 29 g, t dbSNP:778131898
40 40 a, c dbSNP:747621957
43 43 c, g dbSNP:142403358
48 48 a, c, t dbSNP:777152824
49 49 a, g dbSNP:770167215
62 62 g, t dbSNP:775892076
70 70 a, g dbSNP:762917262
75 75 c, t dbSNP:764289618
82 82 a, c dbSNP:200210112
89 89 a, g dbSNP:758018002
96 96 g, t dbSNP:543107439
98 98 c, t dbSNP:746432153
103 103 a, t dbSNP:756540607
109 109 g, t dbSNP:780470109
113 113 c, t dbSNP:749412499
120 120 c, t dbSNP:768944967
126 126 c, t dbSNP:146480945
127 127 -, g dbSNP:750786852
131 131 c, t dbSNP:369696526
138 138 c, g, t dbSNP:576767301
146 146 c, t dbSNP:540982577
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