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CDKN1B cDNA ORF clone, Homo sapiens (human)

Gene Symbol CDKN1B
Entrez Gene ID 1027
Full Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Synonyms CDKN4, KIP1, MEN1B, MEN4, P27KIP1
General protein information
Preferred Names
cyclin-dependent kinase inhibitor 1B
Names
cyclin-dependent kinase inhibitor 1B
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12p13.1-p12

Summary This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]. lac of sum
Disorder MIM:

600778

Disorder Html: Multiple endocrine neoplasia, type IV, 610755 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_004064 NP_004055 cyclin-dependent kinase inhibitor 1B

KEGG
hsa04012 ErbB signaling pathway
hsa04110 Cell cycle
hsa05222 Small cell lung cancer
hsa05220 Chronic myeloid leukemia
hsa05215 Prostate cancer
hsa05200 Pathways in cancer
hsa05162 Measles
hsa05202 Transcriptional misregulation in cancer
hsa05203 Viral carcinogenesis
hsa04151 PI3K-Akt signaling pathway
hsa05161 Hepatitis B
hsa05169 Epstein-Barr virus infection
hsa04066 HIF-1 signaling pathway
hsa04068 FoxO signaling pathway
hsa05206 MicroRNAs in cancer
Reactome
R-HSA-5663202 Diseases of signal transduction
R-HSA-1643685 Disease
R-HSA-2219528 PI3K/AKT Signaling in Cancer
R-HSA-5674400 Constitutive Signaling by AKT1 E17K in Cancer
R-HSA-168256 Immune System
R-HSA-1280218 Adaptive Immune System
R-HSA-983705 Signaling by the B Cell Receptor (BCR)
R-HSA-1168372 Downstream signaling events of B Cell Receptor (BCR)
R-HSA-168249 Innate Immune System
R-HSA-1257604 PIP3 activates AKT signaling
R-HSA-198323 AKT phosphorylates targets in the cytosol
R-HSA-2172127 DAP12 interactions
R-HSA-2424491 DAP12 signaling
R-HSA-2454202 Fc epsilon receptor (FCERI) signaling
R-HSA-2730905 Role of LAT2/NTAL/LAB on calcium mobilization
R-HSA-162582 Signal Transduction
R-HSA-177929 Signaling by EGFR
R-HSA-180292 GAB1 signalosome
R-HSA-5654687 Downstream signaling of activated FGFR1
R-HSA-5654736 Signaling by FGFR1
R-HSA-5654738 Signaling by FGFR2
R-HSA-190236 Signaling by FGFR
R-HSA-5654741 Signaling by FGFR3
R-HSA-5654708 Downstream signaling of activated FGFR3
R-HSA-5654689 PI-3K cascade:FGFR1
R-HSA-5654695 PI-3K cascade:FGFR2
R-HSA-5654743 Signaling by FGFR4
R-HSA-5654720 PI-3K cascade:FGFR4
R-HSA-166520 Signalling by NGF
R-HSA-187037 NGF signalling via TRKA from the plasma membrane
R-HSA-5654716 Downstream signaling of activated FGFR4
R-HSA-5654696 Downstream signaling of activated FGFR2
R-HSA-186797 Signaling by PDGF
R-HSA-186763 Downstream signal transduction
R-HSA-198203 PI3K/AKT activation
R-HSA-5654710 PI-3K cascade:FGFR3
R-HSA-1433557 Signaling by SCF-KIT
R-HSA-1227986 Signaling by ERBB2
R-HSA-1963642 PI3K events in ERBB2 signaling
R-HSA-1236394 Signaling by ERBB4
R-HSA-194315 Signaling by Rho GTPases
R-HSA-195258 RHO GTPase Effectors
R-HSA-1250342 PI3K events in ERBB4 signaling
R-HSA-5625900 RHO GTPases activate CIT
R-HSA-1640170 Cell Cycle
R-HSA-69615 G1/S DNA Damage Checkpoints
R-HSA-69563 p53-Dependent G1 DNA Damage Response
R-HSA-69580 p53-Dependent G1/S DNA damage checkpoint
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-69278 Cell Cycle, Mitotic
R-HSA-69202 Cyclin E associated events during G1/S transition
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-69242 S Phase
R-HSA-69239 Synthesis of DNA
R-HSA-69304 Regulation of DNA replication
R-HSA-69231 Cyclin D associated events in G1
R-HSA-69236 G1 Phase
R-HSA-69300 Removal of licensing factors from origins
R-HSA-69656 Cyclin A:Cdk2-associated events at S phase entry
R-HSA-69052 Switching of origins to a post-replicative state
R-HSA-453279 Mitotic G1-G1/S phases
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69206 G1/S Transition
R-HSA-69306 DNA Replication
R-HSA-2262752 Cellular responses to stress
R-HSA-2559583 Cellular Senescence
R-HSA-2559586 DNA Damage/Telomere Stress Induced Senescence
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
Pathway Interaction Database
hes_heypathway Notch-mediated HES/HEY network
rhoa_pathway RhoA signaling pathway
myc_represspathway Validated targets of C-MYC transcriptional repression
rb_1pathway Regulation of retinoblastoma protein
e2f_pathway E2F transcription factor network
hnf3apathway FOXA1 transcription factor network
telomerasepathway Regulation of Telomerase
avb3_integrin_pathway Integrins in angiogenesis
pi3kciaktpathway Class I PI3K signaling events mediated by Akt
cmyb_pathway C-MYB transcription factor network
foxopathway FoxO family signaling
rhoa_reg_pathway Regulation of RhoA activity
WikiPathways
WP127 IL-5 Signaling Pathway
WP179 Cell cycle
WP707 DNA damage response
WP615 Senescence and Autophagy
WP45 G1 to S cell cycle control
WP34 Ovarian Infertility Genes
WP710 DNA damage response (only ATM dependent)
WP673 ErbB signaling pathway
WP1545 miRNAs involved in DDR
WP2377 Integrated Pancreatic Cancer Pathway
WP2374 Oncostatin M Signaling Pathway
WP2100 AhR pathway
WP2261 Signaling Pathways in Glioblastoma
WP2032 TSH signaling pathway
WP2431 Spinal Cord Injury
WP2446 RB in Cancer


Homo sapiens (human) CDKN1B NP_004055.1
Pan troglodytes (chimpanzee) CDKN1B XP_522347.3
Macaca mulatta (Rhesus monkey) CDKN1B XP_001085433.1
Canis lupus familiaris (dog) CDKN1B NP_001002957.1
Bos taurus (cattle) CDKN1B NP_001093816.1
Mus musculus (house mouse) Cdkn1b NP_034005.2
Rattus norvegicus (Norway rat) Cdkn1b NP_113950.2
Gallus gallus (chicken) CDKN1B NP_989587.1
Danio rerio (zebrafish) cdkn1bb NP_997957.1
Danio rerio (zebrafish) LOC100534692 XP_003201420.1

Component

ID Name Evidence
GO:0005634 nucleus IDA
GO:0005654 nucleoplasm EXP
GO:0005654 nucleoplasm TAS
GO:0005737 cytoplasm IDA
GO:0005768 endosome IEA
GO:0005829 cytosol EXP
GO:0005829 cytosol TAS

Function

ID Name Evidence
GO:0004860 protein kinase inhibitor activity IEA
GO:0004861 cyclin-dependent protein kinase inhibitor activity EXP
GO:0004861 cyclin-dependent protein kinase inhibitor activity TAS
GO:0005072 transforming growth factor beta receptor, cytoplasmic mediator activity TAS
GO:0005515 protein binding IPI
GO:0019903 protein phosphatase binding IPI

Process

ID Name Evidence
GO:0000075 cell cycle checkpoint TAS
GO:0000079 regulation of cyclin-dependent protein kinase activity TAS
GO:0000080 G1 phase of mitotic cell cycle TAS
GO:0000082 G1/S transition of mitotic cell cycle IDA
GO:0000082 G1/S transition of mitotic cell cycle TAS
GO:0000084 S phase of mitotic cell cycle TAS
GO:0000278 mitotic cell cycle TAS
GO:0006813 potassium ion transport IEA
GO:0006917 induction of apoptosis IDA
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest TAS
GO:0007050 cell cycle arrest IMP
GO:0007605 sensory perception of sound IEA
GO:0008284 positive regulation of cell proliferation IEA
GO:0008285 negative regulation of cell proliferation IMP
GO:0030308 negative regulation of cell growth IDA
GO:0031116 positive regulation of microtubule polymerization IEA
GO:0033673 negative regulation of kinase activity IDA
GO:0042326 negative regulation of phosphorylation IDA
GO:0043066 negative regulation of apoptosis IEA
GO:0045732 positive regulation of protein catabolic process IDA
GO:0045736 negative regulation of cyclin-dependent protein kinase activity IEA
GO:0045892 negative regulation of transcription, DNA-dependent IDA
GO:0048011 nerve growth factor receptor signaling pathway TAS
GO:0048015 phosphatidylinositol-mediated signaling TAS
GO:0048102 autophagic cell death IDA
GO:0048839 inner ear development IEA
GO:0051271 negative regulation of cellular component movement IEA
GO:0060770 negative regulation of epithelial cell proliferation involved in prostate gland development IEA
GO:0071236 cellular response to antibiotic IEA
GO:0071285 cellular response to lithium ion IDA
GO:0071407 cellular response to organic cyclic compound IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CDKN1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDKN1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27895
NM_004064 Homo sapiens cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu27895
Accession Version NM_004064.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 597bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cyclin-dependent kinase inhibitor 1B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY097069.1, AK312461.1, BC001971.1, AA833962.1 and HY246392.1. This sequence is a reference standard in the RefSeqGene project. On Feb 12, 2014 this sequence version replaced gi:207113192. Summary: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 12837699 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC001971.1, AY004255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGTCAAACG TGCGAGTGTC TAACGGGAGC CCTAGCCTGG AGCGGATGGA CGCCAGGCAG 
GCGGAGCACC CCAAGCCCTC GGCCTGCAGG AACCTCTTCG GCCCGGTGGA CCACGAAGAG
TTAACCCGGG ACTTGGAGAA GCACTGCAGA GACATGGAAG AGGCGAGCCA GCGCAAGTGG
AATTTCGATT TTCAGAATCA CAAACCCCTA GAGGGCAAGT ACGAGTGGCA AGAGGTGGAG
AAGGGCAGCT TGCCCGAGTT CTACTACAGA CCCCCGCGGC CCCCCAAAGG TGCCTGCAAG
GTGCCGGCGC AGGAGAGCCA GGATGTCAGC GGGAGCCGCC CGGCGGCGCC TTTAATTGGG
GCTCCGGCTA ACTCTGAGGA CACGCATTTG GTGGACCCAA AGACTGATCC GTCGGACAGC
CAGACGGGGT TAGCGGAGCA ATGCGCAGGA ATAAGGAAGC GACCTGCAAC CGACGATTCT
TCTACTCAAA ACAAAAGAGC CAACAGAACA GAAGAAAATG TTTCAGACGG TTCCCCAAAT
GCCGGTTCTG TGGAGCAGAC GCCCAAGAAG CCTGGCCTCA GAAGACGTCA AACGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_004055.1
CDS571..1167
Misc Feature(1)52..141(+)
Misc Feature(2)496..498(+)
Misc Feature(3)598..600(+)
Misc Feature(4)598..600(+)
Misc Feature(5)658..810(+)
Misc Feature(6)790..792(+)
Misc Feature(7)790..792(+)
Misc Feature(8)790..792(+)
Misc Feature(9)832..834(+)
Misc Feature(10)832..834(+)
Misc Feature(11)832..834(+)
Misc Feature(12)832..834(+)
Misc Feature(13)832..834(+)
Misc Feature(14)835..837(+)
Misc Feature(15)988..990(+)
Misc Feature(16)1027..1077(+)
Misc Feature(17)1039..1041(+)
Misc Feature(18)1039..1041(+)
Misc Feature(19)1129..1131(+)
Misc Feature(20)1129..1131(+)
Misc Feature(21)1129..1131(+)
Misc Feature(22)1162..1164(+)
Misc Feature(23)1162..1164(+)
Exon (1)1..1045
Gene:CDKN1B
Gene Synonym:
Exon (2)1046..1175
Gene:CDKN1B
Gene Synonym:
Exon (3)1176..2523
Gene:CDKN1B
Gene Synonym:
Translation
Position Chain Variation Link
14 14 c, g dbSNP:573513680
24 24 a, g dbSNP:540938392
33 33 -, ggc dbSNP:377637464
91 91 a, g dbSNP:754508760
99 99 c, g dbSNP:552816150
102 102 c, t dbSNP:571390693
115 115 -, cctt dbSNP:786201010
115 115 c, t dbSNP:749315608
129 129 a, g dbSNP:577492062
134 134 a, g dbSNP:375987059
160 160 c, t dbSNP:544835565
200 200 c, g dbSNP:11550615
202 202 c, t dbSNP:778339764
254 254 a, g dbSNP:563326398
261 261 c, g dbSNP:747806211
279 279 a, g dbSNP:531078654
305 305 c, g dbSNP:3093728
317 317 c, g dbSNP:561344485
329 329 c, t dbSNP:191541352
359 359 a, g dbSNP:528458910
369 369 c, t dbSNP:183710253
445 445 c, t dbSNP:549943001
465 465 c, t dbSNP:766672713
487 487 c, g dbSNP:115834392
491 491 c, t dbSNP:551236750
492 492 c, t dbSNP:34330
520 520 g, t dbSNP:781293818
523 523 -, t dbSNP:765276743
523 523 g, t dbSNP:750482284
524 524 a, t dbSNP:756141417
535 535 c, g dbSNP:780172282
539 539 -, gaga dbSNP:774454456
542 542 a, g dbSNP:749461130
547 547 c, t dbSNP:368268044
548 548 a, g dbSNP:779324944
549 549 a, g dbSNP:748641280
556 556 a, g dbSNP:772683409
559 559 c, t dbSNP:372535829
560 560 c, t dbSNP:537154637
561 561 c, t dbSNP:374913823
562 562 a, c, g dbSNP:202167434
563 563 a, g dbSNP:763858384
564 564 a, c, g dbSNP:751341214
567 567 a, g dbSNP:767469702
570 570 a, g dbSNP:750323531
573 573 a, g dbSNP:756190836
580 580 g, t dbSNP:780124638
590 590 c, g dbSNP:368157535
594 594 c, t dbSNP:371308246
595 595 a, g dbSNP:755225286
601 601 c, t dbSNP:779193240
602 602 c, g dbSNP:748543504
605 605 a, g, t dbSNP:775772074
606 606 c, t dbSNP:778425357
609 609 c, g dbSNP:534342331
612 612 a, g dbSNP:747456770
613 613 c, t dbSNP:2066828
615 615 c, g dbSNP:775058606
621 621 c, t dbSNP:552533838
624 624 c, t dbSNP:151027466
645 645 a, g dbSNP:367611328
647 647 -, aggcggagcaccccaagcc dbSNP:786201007
648 648 a, c dbSNP:761630889
657 657 c, t dbSNP:767234696
663 663 c, t dbSNP:577554685
669 669 c, t dbSNP:201349921
674 674 c, t dbSNP:375297371
676 676 c, g dbSNP:753931778
684 684 c, t dbSNP:141178987
687 687 -, ag dbSNP:780452142
688 688 a, g dbSNP:765527234
690 690 a, g dbSNP:752899815
691 691 c, t dbSNP:758843248
695 695 c, t dbSNP:200422211
696 696 c, t dbSNP:747582957
699 699 a, c, g dbSNP:757748971
701 701 a, g dbSNP:748854742
712 712 c, t dbSNP:375515128
714 714 c, t dbSNP:774000089
717 717 c, t dbSNP:747742623
719 719 a, g dbSNP:771913043
721 721 g, t dbSNP:369554045
724 724 a, c, t dbSNP:760647541
725 725 g, t dbSNP:543122580
733 733 a, g dbSNP:759665516
734 734 c, g dbSNP:765171184
735 735 a, g dbSNP:16908375
737 737 c, g dbSNP:758686055
738 738 a, c, t dbSNP:35456792
744 744 a, c dbSNP:757517265
747 747 a, g dbSNP:140927518
748 748 c, t dbSNP:746298444
751 751 a, c dbSNP:754531356
761 761 c, t dbSNP:778380972
765 765 g, t dbSNP:747864095
769 769 -, cacaaaccc dbSNP:747343355
771 771 c, t dbSNP:771682338
776 776 c, t dbSNP:777354267
777 777 c, t dbSNP:746849706
782 782 a, t dbSNP:770564585
786 786 c, t dbSNP:776643344
789 789 a, g, t dbSNP:573353679
791 791 a, g dbSNP:775677772
795 795 c, g dbSNP:139727620
797 797 a, g dbSNP:121917832
800 800 a, g dbSNP:764436301
804 804 a, g dbSNP:751794433
810 810 a, g dbSNP:762247829
815 815 a, g dbSNP:767720666
816 816 c, t dbSNP:376624980
818 818 a, g dbSNP:756574571
822 822 c, g dbSNP:778436220
823 823 c, t dbSNP:752181999
828 828 a, g, t dbSNP:142940486
831 831 c, t dbSNP:368696530
834 834 c, t dbSNP:374493057
836 836 a, g dbSNP:780841720
837 837 c, t dbSNP:532903617
841 841 a, c, g, t dbSNP:769828807
842 842 c, g, t dbSNP:768793049
843 843 c, t dbSNP:761928140
846 846 g, t dbSNP:146161282
847 847 c, t dbSNP:140167393
848 848 a, g dbSNP:769996423
849 849 a, g dbSNP:766901538
850 850 c, t dbSNP:146217922
851 851 a, c, t dbSNP:757917082
852 852 c, t dbSNP:751288223
853 853 c, g, t dbSNP:188579132
854 854 c, g, t dbSNP:200141048
855 855 c, t dbSNP:780148172
862 862 a, g dbSNP:749183658
864 864 c, t dbSNP:768919562
869 869 a, g dbSNP:774291520
871 871 a, g dbSNP:748443738
877 877 a, g dbSNP:772163071
878 878 c, t dbSNP:773436050
879 879 a, c, g dbSNP:761029896
890 890 -, a dbSNP:755301027
891 891 g, t dbSNP:370811357
896 896 a, c, g, t dbSNP:2066827
902 902 a, g dbSNP:751007940
906 906 c, t dbSNP:756740369
908 908 a, g dbSNP:767109665
909 909 c, t dbSNP:750028183
911 911 c, g dbSNP:755989911
913 913 g, t dbSNP:779986355
915 915 a, g dbSNP:199570763
919 919 c, t dbSNP:754936421
920 920 c, t dbSNP:779099486
926 926 c, g, t dbSNP:142833529
932 932 c, t dbSNP:201685429
934 934 c, t dbSNP:747217677
935 935 c, t dbSNP:146973564
936 936 c, g dbSNP:776945453
942 942 a, c dbSNP:760081465
943 943 a, t dbSNP:765681672
944 944 -, ct dbSNP:786201011
950 950 a, g dbSNP:749978498
953 953 c, t dbSNP:761235464
956 956 a, g dbSNP:766996989
957 957 c, t dbSNP:377573215
964 964 a, g dbSNP:760403492
967 967 a, c, t dbSNP:137985549
975 975 c, t dbSNP:754756030
977 977 a, g dbSNP:546234840
979 979 a, c dbSNP:752620765
980 980 c, t dbSNP:369871673
981 981 a, g, t dbSNP:534581124
987 987 c, t dbSNP:747272462
995 995 a, c, t dbSNP:771115907
996 996 a, g dbSNP:149775942
999 999 a, g dbSNP:764460528
1005 1005 a, g dbSNP:770131773
1012 1012 c, t dbSNP:775877516
1013 1013 g, t dbSNP:200476090
1015 1015 a, g dbSNP:771553626
1019 1019 c, g dbSNP:772818396
1022 1022 a, t dbSNP:760454287
1029 1029 a, g dbSNP:766153167
1033 1033 c, t dbSNP:753502498
1034 1034 a, c, g dbSNP:143879243
1037 1037 c, t dbSNP:752642842
1039 1039 a, c dbSNP:758325398
1041 1041 a, c dbSNP:777875072
1045 1045 a, g dbSNP:751657844
1049 1049 c, t dbSNP:770629019
1052 1052 c, g, t dbSNP:373917399
1054 1054 a, g dbSNP:769509212
1055 1055 c, t dbSNP:774965131
1062 1062 c, t dbSNP:762810555
1069 1069 a, g dbSNP:763817628
1070 1070 c, t dbSNP:774332981
1073 1073 a, c dbSNP:761736796
1074 1074 c, t dbSNP:767625214
1080 1080 a, g dbSNP:750494869
1088 1088 a, g dbSNP:141509450
1090 1090 a, g dbSNP:766659283
1093 1093 a, t dbSNP:753943702
1095 1095 a, g dbSNP:755410592
1098 1098 c, t dbSNP:779413893
1099 1099 a, g dbSNP:529949605
1100 1100 c, g dbSNP:146167605
1104 1104 c, t dbSNP:778088981
1106 1106 c, t dbSNP:745415005
1113 1113 c, g dbSNP:140177202
1114 1114 a, g dbSNP:775220239
1118 1118 a, c dbSNP:748924503
1121 1121 c, t dbSNP:768507663
1139 1139 a, c dbSNP:774102258
1144 1144 a, g dbSNP:761788198
1146 1146 c, t dbSNP:772023525
1147 1147 c, t dbSNP:73281150
1149 1149 c, t dbSNP:201701670
1156 1156 -, c dbSNP:775142813
1156 1156 c, t dbSNP:766315004
1157 1157 a, g dbSNP:112962540
1159 1159 a, c dbSNP:375717769
1164 1164 a, g dbSNP:759798627
1174 1174 a, c, t dbSNP:200321335
1176 1176 -, aatt dbSNP:760420105
1184 1184 -, at dbSNP:763898281
1184 1184 a, c dbSNP:535558057
1186 1186 a, t dbSNP:760518513
1189 1189 g, t dbSNP:771080235
1193 1193 c, g dbSNP:553872918
1194 1194 -, ttgt dbSNP:776479482
1196 1196 -, t dbSNP:774600817
1202 1202 -, a dbSNP:761706575
1209 1209 a, g dbSNP:776548725
1215 1215 a, g dbSNP:759636349
1236 1236 a, c, g dbSNP:781339295
1237 1237 c, t dbSNP:752899896
1239 1239 c, g dbSNP:763422475
1245 1245 a, c, t dbSNP:764351380
1258 1258 c, g, t dbSNP:757676916
1261 1261 a, g dbSNP:753369668
1263 1263 c, g, t dbSNP:556751200
1264 1264 a, g, t dbSNP:114975362
1267 1267 a, g dbSNP:777396771
1291 1291 a, g dbSNP:747013464
1292 1292 a, t dbSNP:545816667
1304 1304 -, aac dbSNP:765366722
1305 1305 -, c dbSNP:750484441
1311 1311 a, c dbSNP:368340132
1312 1312 c, t dbSNP:745946721
1314 1314 c, g dbSNP:769936824
1316 1316 a, g dbSNP:765213102
1317 1317 c, t dbSNP:557458200
1322 1322 -, ct dbSNP:758412253
1328 1328 -, t dbSNP:766511772
1329 1329 a, t dbSNP:764561406
1337 1337 -, ct dbSNP:751762865
1337 1337 c, t dbSNP:774558574
1340 1340 c, t dbSNP:762188974
1347 1347 a, g dbSNP:767941866
1348 1348 c, t dbSNP:4251696
1363 1363 a, c, g dbSNP:754500857
1364 1364 c, t dbSNP:752416734
1365 1365 c, t dbSNP:543306658
1378 1378 -, a dbSNP:781592070
1380 1380 a, g dbSNP:777431884
1382 1382 a, g dbSNP:374976127
1387 1387 -, g dbSNP:748735564
1426 1426 c, t dbSNP:79141596
1483 1483 c, t dbSNP:201691620
1494 1494 c, t dbSNP:529020970
1502 1502 a, g dbSNP:192945515
1534 1534 a, g dbSNP:1042935
1546 1546 c, t dbSNP:773825212
1588 1588 a, g dbSNP:749773766
1599 1599 g, t dbSNP:146111672
1619 1619 c, t dbSNP:762469235
1620 1620 a, g dbSNP:139019732
1654 1654 a, c dbSNP:551795242
1661 1661 c, g dbSNP:183142712
1668 1668 a, g dbSNP:4251697
1681 1681 a, t dbSNP:549374264
1703 1703 c, t dbSNP:758972461
1777 1777 a, t dbSNP:4251698
1798 1798 g, t dbSNP:535258327
1822 1822 c, t dbSNP:554657425
1872 1872 c, t dbSNP:553587658
1874 1874 a, g dbSNP:775061190
1963 1963 g, t dbSNP:565820084
1972 1972 c, t dbSNP:1042937
1987 1987 c, t dbSNP:539156926
1995 1995 -, cac dbSNP:763451867
1998 1998 a, c dbSNP:760437244
2000 2000 a, t dbSNP:141549130
2002 2002 a, t dbSNP:575907705
2018 2018 c, t dbSNP:763913747
2055 2055 -, a dbSNP:555801286
2088 2088 -, ct dbSNP:574202499
2102 2102 c, t dbSNP:778375300
2116 2116 -, a dbSNP:756729205
2123 2123 a, c dbSNP:7330
2130 2130 c, g dbSNP:555214879
2134 2134 c, t dbSNP:573579359
2147 2147 a, g dbSNP:756925371
2156 2156 g, t dbSNP:765093404
2159 2159 a, g dbSNP:187299006
2211 2211 g, t dbSNP:10237
2224 2224 c, g dbSNP:780883210
2240 2240 c, g dbSNP:751656637
2300 2300 a, t dbSNP:559115148
2320 2320 a, c dbSNP:755073014
2349 2349 a, g dbSNP:578085733
2420 2420 c, t dbSNP:781455059
2424 2424 a, g dbSNP:752902575
2431 2431 a, g dbSNP:192280810
2496 2496 a, t dbSNP:184666485
2519 2519 a, t dbSNP:189357520

Target ORF information:

RefSeq Version NM_004064
Organism Homo sapiens (human)
Definition Homo sapiens cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004064

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTCAAACG TGCGAGTGTC TAACGGGAGC CCTAGCCTGG AGCGGATGGA CGCCAGGCAG 
GCGGAGCACC CCAAGCCCTC GGCCTGCAGG AACCTCTTCG GCCCGGTGGA CCACGAAGAG
TTAACCCGGG ACTTGGAGAA GCACTGCAGA GACATGGAAG AGGCGAGCCA GCGCAAGTGG
AATTTCGATT TTCAGAATCA CAAACCCCTA GAGGGCAAGT ACGAGTGGCA AGAGGTGGAG
AAGGGCAGCT TGCCCGAGTT CTACTACAGA CCCCCGCGGC CCCCCAAAGG TGCCTGCAAG
GTGCCGGCGC AGGAGAGCCA GGATGTCAGC GGGAGCCGCC CGGCGGCGCC TTTAATTGGG
GCTCCGGCTA ACTCTGAGGA CACGCATTTG GTGGACCCAA AGACTGATCC GTCGGACAGC
CAGACGGGGT TAGCGGAGCA ATGCGCAGGA ATAAGGAAGC GACCTGCAAC CGACGATTCT
TCTACTCAAA ACAAAAGAGC CAACAGAACA GAAGAAAATG TTTCAGACGG TTCCCCAAAT
GCCGGTTCTG TGGAGCAGAC GCCCAAGAAG CCTGGCCTCA GAAGACGTCA AACGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

TGF-beta1 stimulates human Tenon's capsule fibroblast proliferation by miR-200b and its targeting of p27/kip1 and RND3
Invest. Ophthalmol. Vis. Sci. 55 (4), 2747-2756 (2014)
Tong J, Fu Y, Xu X, Fan S, Sun H, Liang Y, Xu K, Yuan Z and Ge Y.


book

KLRG1 impairs CD4+ T cell responses via p16ink4a and p27kip1 pathways: role in hepatitis B vaccine failure in individuals with hepatitis C virus infection
J. Immunol. 192 (2), 649-657 (2014)
Shi L, Wang JM, Ren JP, Cheng YQ, Ying RS, Wu XY, Lin SM, Griffin JW, Li GY, Moorman JP and Yao ZQ.


book

Novel functions of core cell cycle regulators in neuronal migration
Adv. Exp. Med. Biol. 800, 59-74 (2014)
Godin JD and Nguyen L.


book

A novel mutation in the upstream open reading frame of the CDKN1B gene causes a MEN4 phenotype
PLoS Genet. 9 (3), E1003350 (2013)
Occhi G, Regazzo D, Trivellin G, Boaretto F, Ciato D, Bobisse S, Ferasin S, Cetani F, Pardi E, Korbonits M, Pellegata NS, Sidarovich V, Quattrone A, Opocher G, Mantero F and Scaroni C.


book

Germ-line mutations in p27Kip1 cause a multiple endocrine neoplasia syndrome in rats and humans
Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15558-15563 (2006)
Pellegata NS, Quintanilla-Martinez L, Siggelkow H, Samson E, Bink K, Hofler H, Fend F, Graw J and Atkinson MJ.


book

Cell cycle-dependent translation of p27 involves a responsive element in its 5'-UTR that overlaps with a uORF
Hum. Mol. Genet. 12 (14), 1767-1779 (2003)
Gopfert U, Kullmann M and Hengst L.


book

ELAV/Hu proteins inhibit p27 translation via an IRES element in the p27 5'UTR
Genes Dev. 16 (23), 3087-3099 (2002)
Kullmann M, Gopfert U, Siewe B and Hengst L.


book

Evidence for different modes of action of cyclin-dependent kinase inhibitors: p15 and p16 bind to kinases, p21 and p27 bind to cyclins
Oncogene 11 (8), 1581-1588 (1995)
Hall M, Bates S and Peters G.


book

Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias
Cancer Res. 55 (6), 1206-1210 (1995)
Pietenpol JA, Bohlander SK, Sato Y, Papadopoulos N, Liu B, Friedman C, Trask BJ, Roberts JM, Kinzler KW and Rowley JD.


book

Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27Kip1, to regions involved in human cancer
Cancer Res. 55 (6), 1199-1205 (1995)
Bullrich F, MacLachlan TK, Sang N, Druck T, Veronese ML, Allen SL, Chiorazzi N, Koff A, Heubner K and Croce CM.


 
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