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CDKN1B cDNA ORF clone, Homo sapiens (human)

Gene Symbol CDKN1B
Entrez Gene ID 1027
Full Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Synonyms CDKN4, KIP1, MEN1B, MEN4, P27KIP1
General protein information
Preferred Names
cyclin-dependent kinase inhibitor 1B
Names
cyclin-dependent kinase inhibitor 1B
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12p13.1-p12

Summary This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]. lac of sum
Disorder MIM:

600778

Disorder Html: Multiple endocrine neoplasia, type IV, 610755 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_004064 NP_004055 cyclin-dependent kinase inhibitor 1B



Homo sapiens (human) CDKN1B NP_004055.1
Pan troglodytes (chimpanzee) CDKN1B XP_522347.3
Macaca mulatta (Rhesus monkey) CDKN1B XP_001085433.1
Canis lupus familiaris (dog) CDKN1B NP_001002957.1
Bos taurus (cattle) CDKN1B NP_001093816.1
Mus musculus (house mouse) Cdkn1b NP_034005.2
Rattus norvegicus (Norway rat) Cdkn1b NP_113950.2
Gallus gallus (chicken) CDKN1B NP_989587.1
Danio rerio (zebrafish) cdkn1bb NP_997957.1
Danio rerio (zebrafish) LOC100534692 XP_003201420.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CDKN1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDKN1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu27895
NM_004064 Homo sapiens cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27895
Clone ID Related Accession (Same CDS sequence) NM_004064
Accession Version NM_004064.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 597bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cyclin-dependent kinase inhibitor 1B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY097069.1, AK312461.1, BC001971.1, AA833962.1 and HY246392.1. This sequence is a reference standard in the RefSeqGene project. On Feb 12, 2014 this sequence version replaced gi:207113192. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 12837699 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC001971.1, AY004255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGTCAAACG TGCGAGTGTC TAACGGGAGC CCTAGCCTGG AGCGGATGGA CGCCAGGCAG 
GCGGAGCACC CCAAGCCCTC GGCCTGCAGG AACCTCTTCG GCCCGGTGGA CCACGAAGAG
TTAACCCGGG ACTTGGAGAA GCACTGCAGA GACATGGAAG AGGCGAGCCA GCGCAAGTGG
AATTTCGATT TTCAGAATCA CAAACCCCTA GAGGGCAAGT ACGAGTGGCA AGAGGTGGAG
AAGGGCAGCT TGCCCGAGTT CTACTACAGA CCCCCGCGGC CCCCCAAAGG TGCCTGCAAG
GTGCCGGCGC AGGAGAGCCA GGATGTCAGC GGGAGCCGCC CGGCGGCGCC TTTAATTGGG
GCTCCGGCTA ACTCTGAGGA CACGCATTTG GTGGACCCAA AGACTGATCC GTCGGACAGC
CAGACGGGGT TAGCGGAGCA ATGCGCAGGA ATAAGGAAGC GACCTGCAAC CGACGATTCT
TCTACTCAAA ACAAAAGAGC CAACAGAACA GAAGAAAATG TTTCAGACGG TTCCCCAAAT
GCCGGTTCTG TGGAGCAGAC GCCCAAGAAG CCTGGCCTCA GAAGACGTCA AACGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004055.1
CDS571..1167
Misc Feature(1)52..141(+)
Misc Feature(2)496..498(+)
Misc Feature(3)598..600(+)
Misc Feature(4)598..600(+)
Misc Feature(5)658..810(+)
Misc Feature(6)790..792(+)
Misc Feature(7)790..792(+)
Misc Feature(8)790..792(+)
Misc Feature(9)832..834(+)
Misc Feature(10)832..834(+)
Misc Feature(11)832..834(+)
Misc Feature(12)832..834(+)
Misc Feature(13)832..834(+)
Misc Feature(14)835..837(+)
Misc Feature(15)988..990(+)
Misc Feature(16)1027..1077(+)
Misc Feature(17)1039..1041(+)
Misc Feature(18)1039..1041(+)
Misc Feature(19)1129..1131(+)
Misc Feature(20)1129..1131(+)
Misc Feature(21)1129..1131(+)
Misc Feature(22)1162..1164(+)
Misc Feature(23)1162..1164(+)
Exon (1)1..1045
Gene:CDKN1B
Gene Synonym:
Exon (2)1046..1175
Gene:CDKN1B
Gene Synonym:
Exon (3)1176..2523
Gene:CDKN1B
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004064
Organism Homo sapiens (human)
Definition Homo sapiens cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004064

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTCAAACG TGCGAGTGTC TAACGGGAGC CCTAGCCTGG AGCGGATGGA CGCCAGGCAG 
GCGGAGCACC CCAAGCCCTC GGCCTGCAGG AACCTCTTCG GCCCGGTGGA CCACGAAGAG
TTAACCCGGG ACTTGGAGAA GCACTGCAGA GACATGGAAG AGGCGAGCCA GCGCAAGTGG
AATTTCGATT TTCAGAATCA CAAACCCCTA GAGGGCAAGT ACGAGTGGCA AGAGGTGGAG
AAGGGCAGCT TGCCCGAGTT CTACTACAGA CCCCCGCGGC CCCCCAAAGG TGCCTGCAAG
GTGCCGGCGC AGGAGAGCCA GGATGTCAGC GGGAGCCGCC CGGCGGCGCC TTTAATTGGG
GCTCCGGCTA ACTCTGAGGA CACGCATTTG GTGGACCCAA AGACTGATCC GTCGGACAGC
CAGACGGGGT TAGCGGAGCA ATGCGCAGGA ATAAGGAAGC GACCTGCAAC CGACGATTCT
TCTACTCAAA ACAAAAGAGC CAACAGAACA GAAGAAAATG TTTCAGACGG TTCCCCAAAT
GCCGGTTCTG TGGAGCAGAC GCCCAAGAAG CCTGGCCTCA GAAGACGTCA AACGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

TGF-beta1 stimulates human Tenon's capsule fibroblast proliferation by miR-200b and its targeting of p27/kip1 and RND3
Invest. Ophthalmol. Vis. Sci. 55 (4), 2747-2756 (2014)
Tong J, Fu Y, Xu X, Fan S, Sun H, Liang Y, Xu K, Yuan Z and Ge Y.


book

KLRG1 impairs CD4+ T cell responses via p16ink4a and p27kip1 pathways: role in hepatitis B vaccine failure in individuals with hepatitis C virus infection
J. Immunol. 192 (2), 649-657 (2014)
Shi L, Wang JM, Ren JP, Cheng YQ, Ying RS, Wu XY, Lin SM, Griffin JW, Li GY, Moorman JP and Yao ZQ.


book

Novel functions of core cell cycle regulators in neuronal migration
Adv. Exp. Med. Biol. 800, 59-74 (2014)
Godin JD and Nguyen L.


book

A novel mutation in the upstream open reading frame of the CDKN1B gene causes a MEN4 phenotype
PLoS Genet. 9 (3), E1003350 (2013)
Occhi G, Regazzo D, Trivellin G, Boaretto F, Ciato D, Bobisse S, Ferasin S, Cetani F, Pardi E, Korbonits M, Pellegata NS, Sidarovich V, Quattrone A, Opocher G, Mantero F and Scaroni C.


book

Germ-line mutations in p27Kip1 cause a multiple endocrine neoplasia syndrome in rats and humans
Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15558-15563 (2006)
Pellegata NS, Quintanilla-Martinez L, Siggelkow H, Samson E, Bink K, Hofler H, Fend F, Graw J and Atkinson MJ.


book

Cell cycle-dependent translation of p27 involves a responsive element in its 5'-UTR that overlaps with a uORF
Hum. Mol. Genet. 12 (14), 1767-1779 (2003)
Gopfert U, Kullmann M and Hengst L.


book

ELAV/Hu proteins inhibit p27 translation via an IRES element in the p27 5'UTR
Genes Dev. 16 (23), 3087-3099 (2002)
Kullmann M, Gopfert U, Siewe B and Hengst L.


book

Evidence for different modes of action of cyclin-dependent kinase inhibitors: p15 and p16 bind to kinases, p21 and p27 bind to cyclins
Oncogene 11 (8), 1581-1588 (1995)
Hall M, Bates S and Peters G.


book

Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias
Cancer Res. 55 (6), 1206-1210 (1995)
Pietenpol JA, Bohlander SK, Sato Y, Papadopoulos N, Liu B, Friedman C, Trask BJ, Roberts JM, Kinzler KW and Rowley JD.


book

Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27Kip1, to regions involved in human cancer
Cancer Res. 55 (6), 1199-1205 (1995)
Bullrich F, MacLachlan TK, Sang N, Druck T, Veronese ML, Allen SL, Chiorazzi N, Koff A, Heubner K and Croce CM.


 
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