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CEBPA cDNA ORF clone, Homo sapiens (human)

Gene Symbol CEBPA
Entrez Gene ID 1050
Full Name CCAAT/enhancer binding protein (C/EBP), alpha
Synonyms C/EBP-alpha, CEBP
General protein information
Preferred Names
CCAAT/enhancer-binding protein alpha
Names
CCAAT/enhancer-binding protein alpha
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

19

19q13.1

Summary This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]. lac of sum
Disorder MIM:

116897

Disorder Html: Leukemia, acute myeloid, 601626 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_004364 NP_004355 CCAAT/enhancer-binding protein alpha isoform a
NM_001287435 NP_001274364 CCAAT/enhancer-binding protein alpha isoform d
NM_001285829 NP_001272758 CCAAT/enhancer-binding protein alpha isoform b
NM_001287424 NP_001274353 CCAAT/enhancer-binding protein alpha isoform c

KEGG
hsa05221 Acute myeloid leukemia
hsa05200 Pathways in cancer
hsa05202 Transcriptional misregulation in cancer
hsa04932 Non-alcoholic fatty liver disease (NAFLD)
Reactome
R-HSA-1266738 Developmental Biology
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation
Pathway Interaction Database
myc_represspathway Validated targets of C-MYC transcriptional repression
rb_1pathway Regulation of retinoblastoma protein
hnf3bpathway FOXA2 and FOXA3 transcription factor networks
e2f_pathway E2F transcription factor network
ar_tf_pathway Regulation of Androgen receptor activity
cmyb_pathway C-MYB transcription factor network
WikiPathways
WP437 EGFR1 Signaling Pathway
WP236 Adipogenesis


Homo sapiens (human) CEBPA NP_001274353.1
Pan troglodytes (chimpanzee) CEBPA XP_001154519.2
Macaca mulatta (Rhesus monkey) LOC717153 XP_001108401.1
Bos taurus (cattle) CEBPA NP_789741.2
Mus musculus (house mouse) Cebpa NP_031704.2
Rattus norvegicus (Norway rat) Cebpa NP_036656.1
Gallus gallus (chicken) CEBPA NP_001026630.1
Danio rerio (zebrafish) cebpa NP_571960.1
Xenopus (Silurana) tropicalis (western clawed frog) cebpa NP_001011044.1

Component

ID Name Evidence
GO:0005634 nucleus NAS
GO:0016363 nuclear matrix IEA
GO:0035189 Rb-E2F complex IEA

Function

ID Name Evidence
GO:0003677 DNA binding TAS
GO:0003700 sequence-specific DNA binding transcription factor activity IDA
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity NAS
GO:0005515 protein binding IPI
GO:0008134 transcription factor binding IPI
GO:0019904 protein domain specific binding IEA
GO:0032403 protein complex binding IEA
GO:0042803 protein homodimerization activity IEA
GO:0043565 sequence-specific DNA binding IEA
GO:0046982 protein heterodimerization activity IEA

Process

ID Name Evidence
GO:0000050 urea cycle IEA
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA
GO:0001889 liver development IEA
GO:0001892 embryonic placenta development IEA
GO:0006091 generation of precursor metabolites and energy TAS
GO:0006351 transcription, DNA-dependent IDA
GO:0006351 transcription, DNA-dependent NAS
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006366 transcription from RNA polymerase II promoter TAS
GO:0006953 acute-phase response IEA
GO:0007005 mitochondrion organization IEA
GO:0007584 response to nutrient IEA
GO:0008203 cholesterol metabolic process IEA
GO:0008285 negative regulation of cell proliferation IEA
GO:0019221 cytokine-mediated signaling pathway NAS
GO:0030099 myeloid cell differentiation NAS
GO:0030225 macrophage differentiation IEA
GO:0030324 lung development IEA
GO:0031100 organ regeneration IEA
GO:0033274 response to vitamin B2 IEA
GO:0044419 interspecies interaction between organisms IEA
GO:0045600 positive regulation of fat cell differentiation IEA
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IEA
GO:0045945 positive regulation of transcription from RNA polymerase III promoter IDA
GO:0048469 cell maturation IEA
GO:0050872 white fat cell differentiation IEA
GO:0050873 brown fat cell differentiation IEA
GO:0051384 response to glucocorticoid stimulus IEA
GO:0071285 cellular response to lithium ion IEA
GO:0071407 cellular response to organic cyclic compound IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CEBPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CEBPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20497
NM_004364 Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$319.00
OHu20906
NM_001287435 Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$319.00
OHu21636
NM_001285829 Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199.00
OHu21531
NM_001287424 Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$319.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu20497
Accession Version NM_004364.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1077bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 01-OCT-2014
Organism Homo sapiens (human)
Product CCAAT/enhancer-binding protein alpha isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008738.7. On Oct 24, 2013 this sequence version replaced gi:225735576. Summary: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]. Transcript Variant: This variant (1) can initiate translation from an upstream non-AUG (GUG) site, and also from three downstream, in-frame AUG sites. The isoform (a, also known as C/EBP-42) represented in this RefSeq results from translation initiation at the first AUG start codon. Isoform a has a shorter N-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 10921906 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGTCGG CCGACTTCTA CGAGGCGGAG CCGCGGCCCC CGATGAGCAG CCACCTGCAG 
AGCCCCCCGC ACGCGCCCAG CAGCGCCGCC TTCGGCTTTC CCCGGGGCGC GGGCCCCGCG
CAGCCTCCCG CCCCACCTGC CGCCCCGGAG CCGCTGGGCG GCATCTGCGA GCACGAGACG
TCCATCGACA TCAGCGCCTA CATCGACCCG GCCGCCTTCA ACGACGAGTT CCTGGCCGAC
CTGTTCCAGC ACAGCCGGCA GCAGGAGAAG GCCAAGGCGG CCGTGGGCCC CACGGGCGGC
GGCGGCGGCG GCGACTTTGA CTACCCGGGC GCGCCCGCGG GCCCCGGCGG CGCCGTCATG
CCCGGGGGAG CGCACGGGCC CCCGCCCGGC TACGGCTGCG CGGCCGCCGG CTACCTGGAC
GGCAGGCTGG AGCCCCTGTA CGAGCGCGTC GGGGCGCCGG CGCTGCGGCC GCTGGTGATC
AAGCAGGAGC CCCGCGAGGA GGATGAAGCC AAGCAGCTGG CGCTGGCCGG CCTCTTCCCT
TACCAGCCGC CGCCGCCGCC GCCGCCCTCG CACCCGCACC CGCACCCGCC GCCCGCGCAC
CTGGCCGCCC CGCACCTGCA GTTCCAGATC GCGCACTGCG GCCAGACCAC CATGCACCTG
CAGCCCGGTC ACCCCACGCC GCCGCCCACG CCCGTGCCCA GCCCGCACCC CGCGCCCGCG
CTCGGTGCCG CCGGCCTGCC GGGCCCTGGC AGCGCGCTCA AGGGGCTGGG CGCCGCGCAC
CCCGACCTCC GCGCGAGTGG CGGCAGCGGC GCGGGCAAGG CCAAGAAGTC GGTGGACAAG
AACAGCAACG AGTACCGGGT GCGGCGCGAG CGCAACAACA TCGCGGTGCG CAAGAGCCGC
GACAAGGCCA AGCAGCGCAA CGTGGAGACG CAGCAGAAGG TGCTGGAGCT GACCAGTGAC
AATGACCGCC TGCGCAAGCG GGTGGAACAG CTGAGCCGCG AACTGGACAC GCTGCGGGGC
ATCTTCCGCC AGCTGCCAGA GAGCTCCTTG GTCAAGGCCA TGGGCAACTG CGCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_004355.2
CDS151..1227
Misc Feature(1)46..48(+)
Misc Feature(2)126..143(+)
Misc Feature(3)193..195(+)
Misc Feature(4)508..510(+)
Misc Feature(5)631..633(+)
Misc Feature(6)631..633(+)
Misc Feature(7)826..828(+)
Misc Feature(8)838..840(+)
Misc Feature(9)850..852(+)
Misc Feature(10)988..1170(+)
Misc Feature(11)994..1170(+)
Misc Feature(12)1003..1062(+)
Misc Feature(13)1006..1089(+)
Misc Feature(14)1069..1167(+)
Misc Feature(15)1099..1185(+)
Exon (1)1..2631
Gene:CEBPA
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, t dbSNP:542791634
41 41 c, t dbSNP:41480346
82 82 a, c dbSNP:557100333
112 112 a, c dbSNP:41434054
180 180 g, t dbSNP:759902033
214 214 c, t dbSNP:770636941
215 215 a, c dbSNP:575182822
218 218 -, c dbSNP:137852729
218 218 -, c dbSNP:137852728
231 231 c, g dbSNP:111662895
257 257 a, g dbSNP:746522150
265 265 -, cccgcgc dbSNP:587776848
291 291 -, c dbSNP:137852730
298 298 g, t dbSNP:121912791
310 310 g, t dbSNP:777090929
334 334 a, g dbSNP:558462037
351 351 -, ctac dbSNP:137852731
360 360 -, gccg dbSNP:587776849
367 367 -, c dbSNP:137852733
401 401 a, t dbSNP:28931590
415 415 -, gag dbSNP:747773004
427 427 a, g dbSNP:758092761
463 463 -, gcg dbSNP:780345232
469 469 -, tg dbSNP:137852732
552 552 a, g dbSNP:752254340
609 609 a, g dbSNP:779373951
646 646 a, g dbSNP:755311871
705 705 -, gccgccgccgcc dbSNP:756538513
705 705 a, g dbSNP:754400471
708 708 -, gccgccgcc dbSNP:746430067
711 711 c, g dbSNP:538441046
714 714 -, gcc dbSNP:781757114
723 723 c, t dbSNP:192240793
739 739 -, acccgc dbSNP:762459325
757 757 g, t dbSNP:750527466
758 758 c, t dbSNP:767444679
762 762 c, g dbSNP:552634598
817 817 a, g dbSNP:535980233
840 840 g, t dbSNP:34529039
843 843 c, g dbSNP:550308123
863 863 a, c dbSNP:3745972
874 874 a, g dbSNP:530569305
904 904 g, t dbSNP:1049967
906 906 g, t dbSNP:571969199
909 909 c, t dbSNP:771005232
926 926 c, t dbSNP:746895795
933 933 c, g dbSNP:777375579
934 934 a, g dbSNP:771453655
936 936 c, t dbSNP:747881336
939 939 c, t dbSNP:552131738
944 944 c, g dbSNP:755401672
948 948 c, t dbSNP:528683666
949 949 a, g dbSNP:749657088
953 953 g, t dbSNP:780335632
961 961 c, g dbSNP:756632245
966 966 c, g dbSNP:750852645
971 971 c, t dbSNP:767673311
974 974 a, g dbSNP:757273086
977 977 a, g dbSNP:201061067
981 981 g, t dbSNP:764419883
990 990 -, gaa dbSNP:764177294
1002 1002 a, g dbSNP:376856647
1025 1025 a, c dbSNP:776590829
1032 1032 c, g, t dbSNP:376093748
1038 1038 a, g dbSNP:772999335
1050 1050 c, g dbSNP:369123206
1059 1059 c, t dbSNP:371444741
1063 1063 a, c dbSNP:747606716
1068 1068 a, c dbSNP:773844593
1079 1079 c, t dbSNP:768306446
1092 1092 g, t dbSNP:377329266
1095 1095 a, g dbSNP:780423965
1100 1100 a, t dbSNP:756436149
1110 1110 c, t dbSNP:746447033
1123 1123 c, t dbSNP:781549846
1126 1126 a, g dbSNP:757720364
1127 1127 a, c dbSNP:543081565
1140 1140 a, g dbSNP:375876732
1147 1147 c, t dbSNP:758726582
1150 1150 a, c, g dbSNP:369632687
1159 1159 a, t dbSNP:587778192
1160 1160 c, t dbSNP:760372994
1168 1168 a, g dbSNP:375833261
1171 1171 a, g dbSNP:372931505
1184 1184 c, t dbSNP:761752002
1190 1190 a, g dbSNP:773869623
1191 1191 a, g dbSNP:141430731
1194 1194 c, g dbSNP:748977615
1201 1201 c, g dbSNP:373210526
1205 1205 a, g dbSNP:775099224
1206 1206 a, g dbSNP:770262748
1215 1215 c, t dbSNP:746226987
1216 1216 a, t dbSNP:543441756
1223 1223 c, g dbSNP:577659401
1229 1229 a, g dbSNP:771363863
1232 1232 c, t dbSNP:375735989
1235 1235 g, t dbSNP:778040955
1238 1238 c, t dbSNP:758531608
1242 1242 a, g dbSNP:753024389
1245 1245 c, t dbSNP:371719077
1247 1247 g, t dbSNP:148740083
1255 1255 c, g dbSNP:750194212
1263 1263 c, t dbSNP:767293321
1264 1264 a, c, g dbSNP:767166906
1267 1267 a, g dbSNP:763641832
1275 1275 a, t dbSNP:762447023
1276 1276 a, g dbSNP:774915318
1296 1296 a, g dbSNP:376092627
1344 1344 c, g dbSNP:572666955
1372 1372 g, t dbSNP:187751931
1387 1387 -, tcct dbSNP:533307993
1389 1389 c, t dbSNP:184965384
1453 1453 a, g dbSNP:753311331
1529 1529 c, t dbSNP:4142943
1541 1541 a, g dbSNP:774356825
1588 1588 a, g dbSNP:41367646
1623 1623 a, c dbSNP:537022582
1625 1625 c, g dbSNP:571299327
1627 1627 -, c dbSNP:568772808
1664 1664 a, g dbSNP:1049969
1771 1771 a, g dbSNP:749251497
1806 1806 c, t dbSNP:192371350
1820 1820 -, c dbSNP:546946624
1822 1822 c, t dbSNP:2376497
1832 1832 c, t dbSNP:372919877
1861 1861 a, g dbSNP:772266008
1884 1884 c, t dbSNP:748249393
1935 1935 c, g dbSNP:549322947
1942 1942 c, g dbSNP:707656
1959 1959 a, g dbSNP:563625019
1967 1967 g, t dbSNP:550273098
1983 1983 c, t dbSNP:533138144
1989 1989 a, g dbSNP:771978125
1995 1995 c, g dbSNP:564155517
2012 2012 c, g dbSNP:544875977
2034 2034 a, g dbSNP:572803705
2051 2051 g, t dbSNP:376667577
2064 2064 a, g dbSNP:528278380
2094 2094 a, g dbSNP:573809108
2107 2107 c, t dbSNP:556765660
2130 2130 c, t dbSNP:562038920
2196 2196 c, t dbSNP:769951978
2221 2221 a, t dbSNP:187516157
2224 2224 g, t dbSNP:748271362
2232 2232 c, t dbSNP:146104564
2260 2260 c, t dbSNP:34017519
2274 2274 g, t dbSNP:535297087
2277 2277 g, t dbSNP:761766986
2288 2288 a, c, g dbSNP:755230057
2289 2289 g, t dbSNP:566231195
2313 2313 c, t dbSNP:113670631
2344 2344 c, t dbSNP:12691
2354 2354 g, t dbSNP:116528776
2443 2443 a, g dbSNP:549827431
2445 2445 c, g dbSNP:576184661
2467 2467 a, c dbSNP:780450509
2478 2478 c, g dbSNP:533270582
2486 2486 c, g dbSNP:564092451
2487 2487 c, g dbSNP:547395645
2507 2507 a, g dbSNP:758844560
2543 2543 c, g dbSNP:35196724
2604 2604 a, t dbSNP:749296829

Target ORF information:

RefSeq Version NM_004364
Organism Homo sapiens (human)
Definition Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004364

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGTCGG CCGACTTCTA CGAGGCGGAG CCGCGGCCCC CGATGAGCAG CCACCTGCAG 
AGCCCCCCGC ACGCGCCCAG CAGCGCCGCC TTCGGCTTTC CCCGGGGCGC GGGCCCCGCG
CAGCCTCCCG CCCCACCTGC CGCCCCGGAG CCGCTGGGCG GCATCTGCGA GCACGAGACG
TCCATCGACA TCAGCGCCTA CATCGACCCG GCCGCCTTCA ACGACGAGTT CCTGGCCGAC
CTGTTCCAGC ACAGCCGGCA GCAGGAGAAG GCCAAGGCGG CCGTGGGCCC CACGGGCGGC
GGCGGCGGCG GCGACTTTGA CTACCCGGGC GCGCCCGCGG GCCCCGGCGG CGCCGTCATG
CCCGGGGGAG CGCACGGGCC CCCGCCCGGC TACGGCTGCG CGGCCGCCGG CTACCTGGAC
GGCAGGCTGG AGCCCCTGTA CGAGCGCGTC GGGGCGCCGG CGCTGCGGCC GCTGGTGATC
AAGCAGGAGC CCCGCGAGGA GGATGAAGCC AAGCAGCTGG CGCTGGCCGG CCTCTTCCCT
TACCAGCCGC CGCCGCCGCC GCCGCCCTCG CACCCGCACC CGCACCCGCC GCCCGCGCAC
CTGGCCGCCC CGCACCTGCA GTTCCAGATC GCGCACTGCG GCCAGACCAC CATGCACCTG
CAGCCCGGTC ACCCCACGCC GCCGCCCACG CCCGTGCCCA GCCCGCACCC CGCGCCCGCG
CTCGGTGCCG CCGGCCTGCC GGGCCCTGGC AGCGCGCTCA AGGGGCTGGG CGCCGCGCAC
CCCGACCTCC GCGCGAGTGG CGGCAGCGGC GCGGGCAAGG CCAAGAAGTC GGTGGACAAG
AACAGCAACG AGTACCGGGT GCGGCGCGAG CGCAACAACA TCGCGGTGCG CAAGAGCCGC
GACAAGGCCA AGCAGCGCAA CGTGGAGACG CAGCAGAAGG TGCTGGAGCT GACCAGTGAC
AATGACCGCC TGCGCAAGCG GGTGGAACAG CTGAGCCGCG AACTGGACAC GCTGCGGGGC
ATCTTCCGCC AGCTGCCAGA GAGCTCCTTG GTCAAGGCCA TGGGCAACTG CGCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20906
Accession Version NM_001287435.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1035bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 01-OCT-2014
Organism Homo sapiens (human)
Product CCAAT/enhancer-binding protein alpha isoform d
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008738.7. Summary: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]. Transcript Variant: This variant (1) can initiate translation from an upstream non-AUG (GUG) site, and also from three downstream, in-frame AUG sites. The isoform (d) represented in this RefSeq results from translation initiation at the second AUG start codon. Isoform d has a shorter N-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:10921906) regulatory uORF :: PMID: 10921906 ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCAGCC ACCTGCAGAG CCCCCCGCAC GCGCCCAGCA GCGCCGCCTT CGGCTTTCCC 
CGGGGCGCGG GCCCCGCGCA GCCTCCCGCC CCACCTGCCG CCCCGGAGCC GCTGGGCGGC
ATCTGCGAGC ACGAGACGTC CATCGACATC AGCGCCTACA TCGACCCGGC CGCCTTCAAC
GACGAGTTCC TGGCCGACCT GTTCCAGCAC AGCCGGCAGC AGGAGAAGGC CAAGGCGGCC
GTGGGCCCCA CGGGCGGCGG CGGCGGCGGC GACTTTGACT ACCCGGGCGC GCCCGCGGGC
CCCGGCGGCG CCGTCATGCC CGGGGGAGCG CACGGGCCCC CGCCCGGCTA CGGCTGCGCG
GCCGCCGGCT ACCTGGACGG CAGGCTGGAG CCCCTGTACG AGCGCGTCGG GGCGCCGGCG
CTGCGGCCGC TGGTGATCAA GCAGGAGCCC CGCGAGGAGG ATGAAGCCAA GCAGCTGGCG
CTGGCCGGCC TCTTCCCTTA CCAGCCGCCG CCGCCGCCGC CGCCCTCGCA CCCGCACCCG
CACCCGCCGC CCGCGCACCT GGCCGCCCCG CACCTGCAGT TCCAGATCGC GCACTGCGGC
CAGACCACCA TGCACCTGCA GCCCGGTCAC CCCACGCCGC CGCCCACGCC CGTGCCCAGC
CCGCACCCCG CGCCCGCGCT CGGTGCCGCC GGCCTGCCGG GCCCTGGCAG CGCGCTCAAG
GGGCTGGGCG CCGCGCACCC CGACCTCCGC GCGAGTGGCG GCAGCGGCGC GGGCAAGGCC
AAGAAGTCGG TGGACAAGAA CAGCAACGAG TACCGGGTGC GGCGCGAGCG CAACAACATC
GCGGTGCGCA AGAGCCGCGA CAAGGCCAAG CAGCGCAACG TGGAGACGCA GCAGAAGGTG
CTGGAGCTGA CCAGTGACAA TGACCGCCTG CGCAAGCGGG TGGAACAGCT GAGCCGCGAA
CTGGACACGC TGCGGGGCAT CTTCCGCCAG CTGCCAGAGA GCTCCTTGGT CAAGGCCATG
GGCAACTGCG CGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001274364.1
CDS193..1227
Misc Feature(1)46..48(+)
Misc Feature(2)126..143(+)
Misc Feature(3)151..153(+)
Misc Feature(4)988..1170(+)
Misc Feature(5)994..1170(+)
Misc Feature(6)1003..1062(+)
Misc Feature(7)1069..1167(+)
Misc Feature(8)508..510(+)
Exon (1)1..2631
Gene:CEBPA
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, t dbSNP:542791634
41 41 c, t dbSNP:41480346
82 82 a, c dbSNP:557100333
112 112 a, c dbSNP:41434054
180 180 g, t dbSNP:759902033
214 214 c, t dbSNP:770636941
215 215 a, c dbSNP:575182822
218 218 -, c dbSNP:137852729
218 218 -, c dbSNP:137852728
231 231 c, g dbSNP:111662895
257 257 a, g dbSNP:746522150
265 265 -, cccgcgc dbSNP:587776848
291 291 -, c dbSNP:137852730
298 298 g, t dbSNP:121912791
310 310 g, t dbSNP:777090929
334 334 a, g dbSNP:558462037
351 351 -, ctac dbSNP:137852731
360 360 -, gccg dbSNP:587776849
367 367 -, c dbSNP:137852733
401 401 a, t dbSNP:28931590
415 415 -, gag dbSNP:747773004
427 427 a, g dbSNP:758092761
463 463 -, gcg dbSNP:780345232
469 469 -, tg dbSNP:137852732
552 552 a, g dbSNP:752254340
609 609 a, g dbSNP:779373951
646 646 a, g dbSNP:755311871
705 705 -, gccgccgccgcc dbSNP:756538513
705 705 a, g dbSNP:754400471
708 708 -, gccgccgcc dbSNP:746430067
711 711 c, g dbSNP:538441046
714 714 -, gcc dbSNP:781757114
723 723 c, t dbSNP:192240793
739 739 -, acccgc dbSNP:762459325
757 757 g, t dbSNP:750527466
758 758 c, t dbSNP:767444679
762 762 c, g dbSNP:552634598
817 817 a, g dbSNP:535980233
840 840 g, t dbSNP:34529039
843 843 c, g dbSNP:550308123
863 863 a, c dbSNP:3745972
874 874 a, g dbSNP:530569305
904 904 g, t dbSNP:1049967
906 906 g, t dbSNP:571969199
909 909 c, t dbSNP:771005232
926 926 c, t dbSNP:746895795
933 933 c, g dbSNP:777375579
934 934 a, g dbSNP:771453655
936 936 c, t dbSNP:747881336
939 939 c, t dbSNP:552131738
944 944 c, g dbSNP:755401672
948 948 c, t dbSNP:528683666
949 949 a, g dbSNP:749657088
953 953 g, t dbSNP:780335632
961 961 c, g dbSNP:756632245
966 966 c, g dbSNP:750852645
971 971 c, t dbSNP:767673311
974 974 a, g dbSNP:757273086
977 977 a, g dbSNP:201061067
981 981 g, t dbSNP:764419883
990 990 -, gaa dbSNP:764177294
1002 1002 a, g dbSNP:376856647
1025 1025 a, c dbSNP:776590829
1032 1032 c, g, t dbSNP:376093748
1038 1038 a, g dbSNP:772999335
1050 1050 c, g dbSNP:369123206
1059 1059 c, t dbSNP:371444741
1063 1063 a, c dbSNP:747606716
1068 1068 a, c dbSNP:773844593
1079 1079 c, t dbSNP:768306446
1092 1092 g, t dbSNP:377329266
1095 1095 a, g dbSNP:780423965
1100 1100 a, t dbSNP:756436149
1110 1110 c, t dbSNP:746447033
1123 1123 c, t dbSNP:781549846
1126 1126 a, g dbSNP:757720364
1127 1127 a, c dbSNP:543081565
1140 1140 a, g dbSNP:375876732
1147 1147 c, t dbSNP:758726582
1150 1150 a, c, g dbSNP:369632687
1159 1159 a, t dbSNP:587778192
1160 1160 c, t dbSNP:760372994
1168 1168 a, g dbSNP:375833261
1171 1171 a, g dbSNP:372931505
1184 1184 c, t dbSNP:761752002
1190 1190 a, g dbSNP:773869623
1191 1191 a, g dbSNP:141430731
1194 1194 c, g dbSNP:748977615
1201 1201 c, g dbSNP:373210526
1205 1205 a, g dbSNP:775099224
1206 1206 a, g dbSNP:770262748
1215 1215 c, t dbSNP:746226987
1216 1216 a, t dbSNP:543441756
1223 1223 c, g dbSNP:577659401
1229 1229 a, g dbSNP:771363863
1232 1232 c, t dbSNP:375735989
1235 1235 g, t dbSNP:778040955
1238 1238 c, t dbSNP:758531608
1242 1242 a, g dbSNP:753024389
1245 1245 c, t dbSNP:371719077
1247 1247 g, t dbSNP:148740083
1255 1255 c, g dbSNP:750194212
1263 1263 c, t dbSNP:767293321
1264 1264 a, c, g dbSNP:767166906
1267 1267 a, g dbSNP:763641832
1275 1275 a, t dbSNP:762447023
1276 1276 a, g dbSNP:774915318
1296 1296 a, g dbSNP:376092627
1344 1344 c, g dbSNP:572666955
1372 1372 g, t dbSNP:187751931
1387 1387 -, tcct dbSNP:533307993
1389 1389 c, t dbSNP:184965384
1453 1453 a, g dbSNP:753311331
1529 1529 c, t dbSNP:4142943
1541 1541 a, g dbSNP:774356825
1588 1588 a, g dbSNP:41367646
1623 1623 a, c dbSNP:537022582
1625 1625 c, g dbSNP:571299327
1627 1627 -, c dbSNP:568772808
1664 1664 a, g dbSNP:1049969
1771 1771 a, g dbSNP:749251497
1806 1806 c, t dbSNP:192371350
1820 1820 -, c dbSNP:546946624
1822 1822 c, t dbSNP:2376497
1832 1832 c, t dbSNP:372919877
1861 1861 a, g dbSNP:772266008
1884 1884 c, t dbSNP:748249393
1935 1935 c, g dbSNP:549322947
1942 1942 c, g dbSNP:707656
1959 1959 a, g dbSNP:563625019
1967 1967 g, t dbSNP:550273098
1983 1983 c, t dbSNP:533138144
1989 1989 a, g dbSNP:771978125
1995 1995 c, g dbSNP:564155517
2012 2012 c, g dbSNP:544875977
2034 2034 a, g dbSNP:572803705
2051 2051 g, t dbSNP:376667577
2064 2064 a, g dbSNP:528278380
2094 2094 a, g dbSNP:573809108
2107 2107 c, t dbSNP:556765660
2130 2130 c, t dbSNP:562038920
2196 2196 c, t dbSNP:769951978
2221 2221 a, t dbSNP:187516157
2224 2224 g, t dbSNP:748271362
2232 2232 c, t dbSNP:146104564
2260 2260 c, t dbSNP:34017519
2274 2274 g, t dbSNP:535297087
2277 2277 g, t dbSNP:761766986
2288 2288 a, c, g dbSNP:755230057
2289 2289 g, t dbSNP:566231195
2313 2313 c, t dbSNP:113670631
2344 2344 c, t dbSNP:12691
2354 2354 g, t dbSNP:116528776
2443 2443 a, g dbSNP:549827431
2445 2445 c, g dbSNP:576184661
2467 2467 a, c dbSNP:780450509
2478 2478 c, g dbSNP:533270582
2486 2486 c, g dbSNP:564092451
2487 2487 c, g dbSNP:547395645
2507 2507 a, g dbSNP:758844560
2543 2543 c, g dbSNP:35196724
2604 2604 a, t dbSNP:749296829

Target ORF information:

RefSeq Version NM_001287435
Organism Homo sapiens (human)
Definition Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287435

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCAGCC ACCTGCAGAG CCCCCCGCAC GCGCCCAGCA GCGCCGCCTT CGGCTTTCCC 
CGGGGCGCGG GCCCCGCGCA GCCTCCCGCC CCACCTGCCG CCCCGGAGCC GCTGGGCGGC
ATCTGCGAGC ACGAGACGTC CATCGACATC AGCGCCTACA TCGACCCGGC CGCCTTCAAC
GACGAGTTCC TGGCCGACCT GTTCCAGCAC AGCCGGCAGC AGGAGAAGGC CAAGGCGGCC
GTGGGCCCCA CGGGCGGCGG CGGCGGCGGC GACTTTGACT ACCCGGGCGC GCCCGCGGGC
CCCGGCGGCG CCGTCATGCC CGGGGGAGCG CACGGGCCCC CGCCCGGCTA CGGCTGCGCG
GCCGCCGGCT ACCTGGACGG CAGGCTGGAG CCCCTGTACG AGCGCGTCGG GGCGCCGGCG
CTGCGGCCGC TGGTGATCAA GCAGGAGCCC CGCGAGGAGG ATGAAGCCAA GCAGCTGGCG
CTGGCCGGCC TCTTCCCTTA CCAGCCGCCG CCGCCGCCGC CGCCCTCGCA CCCGCACCCG
CACCCGCCGC CCGCGCACCT GGCCGCCCCG CACCTGCAGT TCCAGATCGC GCACTGCGGC
CAGACCACCA TGCACCTGCA GCCCGGTCAC CCCACGCCGC CGCCCACGCC CGTGCCCAGC
CCGCACCCCG CGCCCGCGCT CGGTGCCGCC GGCCTGCCGG GCCCTGGCAG CGCGCTCAAG
GGGCTGGGCG CCGCGCACCC CGACCTCCGC GCGAGTGGCG GCAGCGGCGC GGGCAAGGCC
AAGAAGTCGG TGGACAAGAA CAGCAACGAG TACCGGGTGC GGCGCGAGCG CAACAACATC
GCGGTGCGCA AGAGCCGCGA CAAGGCCAAG CAGCGCAACG TGGAGACGCA GCAGAAGGTG
CTGGAGCTGA CCAGTGACAA TGACCGCCTG CGCAAGCGGG TGGAACAGCT GAGCCGCGAA
CTGGACACGC TGCGGGGCAT CTTCCGCCAG CTGCCAGAGA GCTCCTTGGT CAAGGCCATG
GGCAACTGCG CGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21636
Accession Version NM_001285829.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 720bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 01-OCT-2014
Organism Homo sapiens (human)
Product CCAAT/enhancer-binding protein alpha isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008738.7. Summary: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]. Transcript Variant: This variant (1) can initiate translation from an upstream non-AUG (GUG) site, and also from three downstream, in-frame AUG sites. The isoform (b, also known as C/EBP-30) represented in this RefSeq results from translation initiation at the third AUG start codon. Isoform b has a shorter N-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:10921906) regulatory uORF :: PMID: 10921906 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGCCCGGGG GAGCGCACGG GCCCCCGCCC GGCTACGGCT GCGCGGCCGC CGGCTACCTG 
GACGGCAGGC TGGAGCCCCT GTACGAGCGC GTCGGGGCGC CGGCGCTGCG GCCGCTGGTG
ATCAAGCAGG AGCCCCGCGA GGAGGATGAA GCCAAGCAGC TGGCGCTGGC CGGCCTCTTC
CCTTACCAGC CGCCGCCGCC GCCGCCGCCC TCGCACCCGC ACCCGCACCC GCCGCCCGCG
CACCTGGCCG CCCCGCACCT GCAGTTCCAG ATCGCGCACT GCGGCCAGAC CACCATGCAC
CTGCAGCCCG GTCACCCCAC GCCGCCGCCC ACGCCCGTGC CCAGCCCGCA CCCCGCGCCC
GCGCTCGGTG CCGCCGGCCT GCCGGGCCCT GGCAGCGCGC TCAAGGGGCT GGGCGCCGCG
CACCCCGACC TCCGCGCGAG TGGCGGCAGC GGCGCGGGCA AGGCCAAGAA GTCGGTGGAC
AAGAACAGCA ACGAGTACCG GGTGCGGCGC GAGCGCAACA ACATCGCGGT GCGCAAGAGC
CGCGACAAGG CCAAGCAGCG CAACGTGGAG ACGCAGCAGA AGGTGCTGGA GCTGACCAGT
GACAATGACC GCCTGCGCAA GCGGGTGGAA CAGCTGAGCC GCGAACTGGA CACGCTGCGG
GGCATCTTCC GCCAGCTGCC AGAGAGCTCC TTGGTCAAGG CCATGGGCAA CTGCGCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001272758.1
CDS508..1227
Misc Feature(1)46..48(+)
Misc Feature(2)126..143(+)
Misc Feature(3)151..153(+)
Misc Feature(4)193..195(+)
Misc Feature(5)988..1170(+)
Misc Feature(6)994..1170(+)
Misc Feature(7)1003..1062(+)
Misc Feature(8)1069..1167(+)
Exon (1)1..2631
Gene:CEBPA
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, t dbSNP:542791634
41 41 c, t dbSNP:41480346
82 82 a, c dbSNP:557100333
112 112 a, c dbSNP:41434054
180 180 g, t dbSNP:759902033
214 214 c, t dbSNP:770636941
215 215 a, c dbSNP:575182822
218 218 -, c dbSNP:137852729
218 218 -, c dbSNP:137852728
231 231 c, g dbSNP:111662895
257 257 a, g dbSNP:746522150
265 265 -, cccgcgc dbSNP:587776848
291 291 -, c dbSNP:137852730
298 298 g, t dbSNP:121912791
310 310 g, t dbSNP:777090929
334 334 a, g dbSNP:558462037
351 351 -, ctac dbSNP:137852731
360 360 -, gccg dbSNP:587776849
367 367 -, c dbSNP:137852733
401 401 a, t dbSNP:28931590
415 415 -, gag dbSNP:747773004
427 427 a, g dbSNP:758092761
463 463 -, gcg dbSNP:780345232
469 469 -, tg dbSNP:137852732
552 552 a, g dbSNP:752254340
609 609 a, g dbSNP:779373951
646 646 a, g dbSNP:755311871
705 705 -, gccgccgccgcc dbSNP:756538513
705 705 a, g dbSNP:754400471
708 708 -, gccgccgcc dbSNP:746430067
711 711 c, g dbSNP:538441046
714 714 -, gcc dbSNP:781757114
723 723 c, t dbSNP:192240793
739 739 -, acccgc dbSNP:762459325
757 757 g, t dbSNP:750527466
758 758 c, t dbSNP:767444679
762 762 c, g dbSNP:552634598
817 817 a, g dbSNP:535980233
840 840 g, t dbSNP:34529039
843 843 c, g dbSNP:550308123
863 863 a, c dbSNP:3745972
874 874 a, g dbSNP:530569305
904 904 g, t dbSNP:1049967
906 906 g, t dbSNP:571969199
909 909 c, t dbSNP:771005232
926 926 c, t dbSNP:746895795
933 933 c, g dbSNP:777375579
934 934 a, g dbSNP:771453655
936 936 c, t dbSNP:747881336
939 939 c, t dbSNP:552131738
944 944 c, g dbSNP:755401672
948 948 c, t dbSNP:528683666
949 949 a, g dbSNP:749657088
953 953 g, t dbSNP:780335632
961 961 c, g dbSNP:756632245
966 966 c, g dbSNP:750852645
971 971 c, t dbSNP:767673311
974 974 a, g dbSNP:757273086
977 977 a, g dbSNP:201061067
981 981 g, t dbSNP:764419883
990 990 -, gaa dbSNP:764177294
1002 1002 a, g dbSNP:376856647
1025 1025 a, c dbSNP:776590829
1032 1032 c, g, t dbSNP:376093748
1038 1038 a, g dbSNP:772999335
1050 1050 c, g dbSNP:369123206
1059 1059 c, t dbSNP:371444741
1063 1063 a, c dbSNP:747606716
1068 1068 a, c dbSNP:773844593
1079 1079 c, t dbSNP:768306446
1092 1092 g, t dbSNP:377329266
1095 1095 a, g dbSNP:780423965
1100 1100 a, t dbSNP:756436149
1110 1110 c, t dbSNP:746447033
1123 1123 c, t dbSNP:781549846
1126 1126 a, g dbSNP:757720364
1127 1127 a, c dbSNP:543081565
1140 1140 a, g dbSNP:375876732
1147 1147 c, t dbSNP:758726582
1150 1150 a, c, g dbSNP:369632687
1159 1159 a, t dbSNP:587778192
1160 1160 c, t dbSNP:760372994
1168 1168 a, g dbSNP:375833261
1171 1171 a, g dbSNP:372931505
1184 1184 c, t dbSNP:761752002
1190 1190 a, g dbSNP:773869623
1191 1191 a, g dbSNP:141430731
1194 1194 c, g dbSNP:748977615
1201 1201 c, g dbSNP:373210526
1205 1205 a, g dbSNP:775099224
1206 1206 a, g dbSNP:770262748
1215 1215 c, t dbSNP:746226987
1216 1216 a, t dbSNP:543441756
1223 1223 c, g dbSNP:577659401
1229 1229 a, g dbSNP:771363863
1232 1232 c, t dbSNP:375735989
1235 1235 g, t dbSNP:778040955
1238 1238 c, t dbSNP:758531608
1242 1242 a, g dbSNP:753024389
1245 1245 c, t dbSNP:371719077
1247 1247 g, t dbSNP:148740083
1255 1255 c, g dbSNP:750194212
1263 1263 c, t dbSNP:767293321
1264 1264 a, c, g dbSNP:767166906
1267 1267 a, g dbSNP:763641832
1275 1275 a, t dbSNP:762447023
1276 1276 a, g dbSNP:774915318
1296 1296 a, g dbSNP:376092627
1344 1344 c, g dbSNP:572666955
1372 1372 g, t dbSNP:187751931
1387 1387 -, tcct dbSNP:533307993
1389 1389 c, t dbSNP:184965384
1453 1453 a, g dbSNP:753311331
1529 1529 c, t dbSNP:4142943
1541 1541 a, g dbSNP:774356825
1588 1588 a, g dbSNP:41367646
1623 1623 a, c dbSNP:537022582
1625 1625 c, g dbSNP:571299327
1627 1627 -, c dbSNP:568772808
1664 1664 a, g dbSNP:1049969
1771 1771 a, g dbSNP:749251497
1806 1806 c, t dbSNP:192371350
1820 1820 -, c dbSNP:546946624
1822 1822 c, t dbSNP:2376497
1832 1832 c, t dbSNP:372919877
1861 1861 a, g dbSNP:772266008
1884 1884 c, t dbSNP:748249393
1935 1935 c, g dbSNP:549322947
1942 1942 c, g dbSNP:707656
1959 1959 a, g dbSNP:563625019
1967 1967 g, t dbSNP:550273098
1983 1983 c, t dbSNP:533138144
1989 1989 a, g dbSNP:771978125
1995 1995 c, g dbSNP:564155517
2012 2012 c, g dbSNP:544875977
2034 2034 a, g dbSNP:572803705
2051 2051 g, t dbSNP:376667577
2064 2064 a, g dbSNP:528278380
2094 2094 a, g dbSNP:573809108
2107 2107 c, t dbSNP:556765660
2130 2130 c, t dbSNP:562038920
2196 2196 c, t dbSNP:769951978
2221 2221 a, t dbSNP:187516157
2224 2224 g, t dbSNP:748271362
2232 2232 c, t dbSNP:146104564
2260 2260 c, t dbSNP:34017519
2274 2274 g, t dbSNP:535297087
2277 2277 g, t dbSNP:761766986
2288 2288 a, c, g dbSNP:755230057
2289 2289 g, t dbSNP:566231195
2313 2313 c, t dbSNP:113670631
2344 2344 c, t dbSNP:12691
2354 2354 g, t dbSNP:116528776
2443 2443 a, g dbSNP:549827431
2445 2445 c, g dbSNP:576184661
2467 2467 a, c dbSNP:780450509
2478 2478 c, g dbSNP:533270582
2486 2486 c, g dbSNP:564092451
2487 2487 c, g dbSNP:547395645
2507 2507 a, g dbSNP:758844560
2543 2543 c, g dbSNP:35196724
2604 2604 a, t dbSNP:749296829

Target ORF information:

RefSeq Version NM_001285829
Organism Homo sapiens (human)
Definition Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001285829

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCCCGGGG GAGCGCACGG GCCCCCGCCC GGCTACGGCT GCGCGGCCGC CGGCTACCTG 
GACGGCAGGC TGGAGCCCCT GTACGAGCGC GTCGGGGCGC CGGCGCTGCG GCCGCTGGTG
ATCAAGCAGG AGCCCCGCGA GGAGGATGAA GCCAAGCAGC TGGCGCTGGC CGGCCTCTTC
CCTTACCAGC CGCCGCCGCC GCCGCCGCCC TCGCACCCGC ACCCGCACCC GCCGCCCGCG
CACCTGGCCG CCCCGCACCT GCAGTTCCAG ATCGCGCACT GCGGCCAGAC CACCATGCAC
CTGCAGCCCG GTCACCCCAC GCCGCCGCCC ACGCCCGTGC CCAGCCCGCA CCCCGCGCCC
GCGCTCGGTG CCGCCGGCCT GCCGGGCCCT GGCAGCGCGC TCAAGGGGCT GGGCGCCGCG
CACCCCGACC TCCGCGCGAG TGGCGGCAGC GGCGCGGGCA AGGCCAAGAA GTCGGTGGAC
AAGAACAGCA ACGAGTACCG GGTGCGGCGC GAGCGCAACA ACATCGCGGT GCGCAAGAGC
CGCGACAAGG CCAAGCAGCG CAACGTGGAG ACGCAGCAGA AGGTGCTGGA GCTGACCAGT
GACAATGACC GCCTGCGCAA GCGGGTGGAA CAGCTGAGCC GCGAACTGGA CACGCTGCGG
GGCATCTTCC GCCAGCTGCC AGAGAGCTCC TTGGTCAAGG CCATGGGCAA CTGCGCGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21531
Accession Version NM_001287424.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 01-OCT-2014
Organism Homo sapiens (human)
Product CCAAT/enhancer-binding protein alpha isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008738.7. Summary: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]. Transcript Variant: This variant (1) can initiate translation from an upstream non-AUG (GUG) site, and also from three downstream, in-frame AUG sites. The isoform (c, also known as extended) represented in this RefSeq results from translation initiation at the upstream GUG start codon. Isoform c is the longest isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 20075868 ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
GTGCGCGGGC GCGGGCGAGC AGGGTCTCCG GGTGGGCGGC GGCGACGCCC CGCGCAGGCT 
GGAGGCCGCC GAGGCTCGCC ATGCCGGGAG AACTCTAACT CCCCCATGGA GTCGGCCGAC
TTCTACGAGG CGGAGCCGCG GCCCCCGATG AGCAGCCACC TGCAGAGCCC CCCGCACGCG
CCCAGCAGCG CCGCCTTCGG CTTTCCCCGG GGCGCGGGCC CCGCGCAGCC TCCCGCCCCA
CCTGCCGCCC CGGAGCCGCT GGGCGGCATC TGCGAGCACG AGACGTCCAT CGACATCAGC
GCCTACATCG ACCCGGCCGC CTTCAACGAC GAGTTCCTGG CCGACCTGTT CCAGCACAGC
CGGCAGCAGG AGAAGGCCAA GGCGGCCGTG GGCCCCACGG GCGGCGGCGG CGGCGGCGAC
TTTGACTACC CGGGCGCGCC CGCGGGCCCC GGCGGCGCCG TCATGCCCGG GGGAGCGCAC
GGGCCCCCGC CCGGCTACGG CTGCGCGGCC GCCGGCTACC TGGACGGCAG GCTGGAGCCC
CTGTACGAGC GCGTCGGGGC GCCGGCGCTG CGGCCGCTGG TGATCAAGCA GGAGCCCCGC
GAGGAGGATG AAGCCAAGCA GCTGGCGCTG GCCGGCCTCT TCCCTTACCA GCCGCCGCCG
CCGCCGCCGC CCTCGCACCC GCACCCGCAC CCGCCGCCCG CGCACCTGGC CGCCCCGCAC
CTGCAGTTCC AGATCGCGCA CTGCGGCCAG ACCACCATGC ACCTGCAGCC CGGTCACCCC
ACGCCGCCGC CCACGCCCGT GCCCAGCCCG CACCCCGCGC CCGCGCTCGG TGCCGCCGGC
CTGCCGGGCC CTGGCAGCGC GCTCAAGGGG CTGGGCGCCG CGCACCCCGA CCTCCGCGCG
AGTGGCGGCA GCGGCGCGGG CAAGGCCAAG AAGTCGGTGG ACAAGAACAG CAACGAGTAC
CGGGTGCGGC GCGAGCGCAA CAACATCGCG GTGCGCAAGA GCCGCGACAA GGCCAAGCAG
CGCAACGTGG AGACGCAGCA GAAGGTGCTG GAGCTGACCA GTGACAATGA CCGCCTGCGC
AAGCGGGTGG AACAGCTGAG CCGCGAACTG GACACGCTGC GGGGCATCTT CCGCCAGCTG
CCAGAGAGCT CCTTGGTCAA GGCCATGGGC AACTGCGCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001274353.1
CDS46..1227
Misc Feature(1)988..1170(+)
Misc Feature(2)994..1170(+)
Misc Feature(3)1003..1062(+)
Misc Feature(4)1069..1167(+)
Misc Feature(5)151..153(+)
Misc Feature(6)193..195(+)
Misc Feature(7)508..510(+)
Exon (1)1..2631
Gene:CEBPA
Gene Synonym:
Translation
Position Chain Variation Link
24 24 c, t dbSNP:542791634
41 41 c, t dbSNP:41480346
82 82 a, c dbSNP:557100333
112 112 a, c dbSNP:41434054
180 180 g, t dbSNP:759902033
214 214 c, t dbSNP:770636941
215 215 a, c dbSNP:575182822
218 218 -, c dbSNP:137852729
218 218 -, c dbSNP:137852728
231 231 c, g dbSNP:111662895
257 257 a, g dbSNP:746522150
265 265 -, cccgcgc dbSNP:587776848
291 291 -, c dbSNP:137852730
298 298 g, t dbSNP:121912791
310 310 g, t dbSNP:777090929
334 334 a, g dbSNP:558462037
351 351 -, ctac dbSNP:137852731
360 360 -, gccg dbSNP:587776849
367 367 -, c dbSNP:137852733
401 401 a, t dbSNP:28931590
415 415 -, gag dbSNP:747773004
427 427 a, g dbSNP:758092761
463 463 -, gcg dbSNP:780345232
469 469 -, tg dbSNP:137852732
552 552 a, g dbSNP:752254340
609 609 a, g dbSNP:779373951
646 646 a, g dbSNP:755311871
705 705 -, gccgccgccgcc dbSNP:756538513
705 705 a, g dbSNP:754400471
708 708 -, gccgccgcc dbSNP:746430067
711 711 c, g dbSNP:538441046
714 714 -, gcc dbSNP:781757114
723 723 c, t dbSNP:192240793
739 739 -, acccgc dbSNP:762459325
757 757 g, t dbSNP:750527466
758 758 c, t dbSNP:767444679
762 762 c, g dbSNP:552634598
817 817 a, g dbSNP:535980233
840 840 g, t dbSNP:34529039
843 843 c, g dbSNP:550308123
863 863 a, c dbSNP:3745972
874 874 a, g dbSNP:530569305
904 904 g, t dbSNP:1049967
906 906 g, t dbSNP:571969199
909 909 c, t dbSNP:771005232
926 926 c, t dbSNP:746895795
933 933 c, g dbSNP:777375579
934 934 a, g dbSNP:771453655
936 936 c, t dbSNP:747881336
939 939 c, t dbSNP:552131738
944 944 c, g dbSNP:755401672
948 948 c, t dbSNP:528683666
949 949 a, g dbSNP:749657088
953 953 g, t dbSNP:780335632
961 961 c, g dbSNP:756632245
966 966 c, g dbSNP:750852645
971 971 c, t dbSNP:767673311
974 974 a, g dbSNP:757273086
977 977 a, g dbSNP:201061067
981 981 g, t dbSNP:764419883
990 990 -, gaa dbSNP:764177294
1002 1002 a, g dbSNP:376856647
1025 1025 a, c dbSNP:776590829
1032 1032 c, g, t dbSNP:376093748
1038 1038 a, g dbSNP:772999335
1050 1050 c, g dbSNP:369123206
1059 1059 c, t dbSNP:371444741
1063 1063 a, c dbSNP:747606716
1068 1068 a, c dbSNP:773844593
1079 1079 c, t dbSNP:768306446
1092 1092 g, t dbSNP:377329266
1095 1095 a, g dbSNP:780423965
1100 1100 a, t dbSNP:756436149
1110 1110 c, t dbSNP:746447033
1123 1123 c, t dbSNP:781549846
1126 1126 a, g dbSNP:757720364
1127 1127 a, c dbSNP:543081565
1140 1140 a, g dbSNP:375876732
1147 1147 c, t dbSNP:758726582
1150 1150 a, c, g dbSNP:369632687
1159 1159 a, t dbSNP:587778192
1160 1160 c, t dbSNP:760372994
1168 1168 a, g dbSNP:375833261
1171 1171 a, g dbSNP:372931505
1184 1184 c, t dbSNP:761752002
1190 1190 a, g dbSNP:773869623
1191 1191 a, g dbSNP:141430731
1194 1194 c, g dbSNP:748977615
1201 1201 c, g dbSNP:373210526
1205 1205 a, g dbSNP:775099224
1206 1206 a, g dbSNP:770262748
1215 1215 c, t dbSNP:746226987
1216 1216 a, t dbSNP:543441756
1223 1223 c, g dbSNP:577659401
1229 1229 a, g dbSNP:771363863
1232 1232 c, t dbSNP:375735989
1235 1235 g, t dbSNP:778040955
1238 1238 c, t dbSNP:758531608
1242 1242 a, g dbSNP:753024389
1245 1245 c, t dbSNP:371719077
1247 1247 g, t dbSNP:148740083
1255 1255 c, g dbSNP:750194212
1263 1263 c, t dbSNP:767293321
1264 1264 a, c, g dbSNP:767166906
1267 1267 a, g dbSNP:763641832
1275 1275 a, t dbSNP:762447023
1276 1276 a, g dbSNP:774915318
1296 1296 a, g dbSNP:376092627
1344 1344 c, g dbSNP:572666955
1372 1372 g, t dbSNP:187751931
1387 1387 -, tcct dbSNP:533307993
1389 1389 c, t dbSNP:184965384
1453 1453 a, g dbSNP:753311331
1529 1529 c, t dbSNP:4142943
1541 1541 a, g dbSNP:774356825
1588 1588 a, g dbSNP:41367646
1623 1623 a, c dbSNP:537022582
1625 1625 c, g dbSNP:571299327
1627 1627 -, c dbSNP:568772808
1664 1664 a, g dbSNP:1049969
1771 1771 a, g dbSNP:749251497
1806 1806 c, t dbSNP:192371350
1820 1820 -, c dbSNP:546946624
1822 1822 c, t dbSNP:2376497
1832 1832 c, t dbSNP:372919877
1861 1861 a, g dbSNP:772266008
1884 1884 c, t dbSNP:748249393
1935 1935 c, g dbSNP:549322947
1942 1942 c, g dbSNP:707656
1959 1959 a, g dbSNP:563625019
1967 1967 g, t dbSNP:550273098
1983 1983 c, t dbSNP:533138144
1989 1989 a, g dbSNP:771978125
1995 1995 c, g dbSNP:564155517
2012 2012 c, g dbSNP:544875977
2034 2034 a, g dbSNP:572803705
2051 2051 g, t dbSNP:376667577
2064 2064 a, g dbSNP:528278380
2094 2094 a, g dbSNP:573809108
2107 2107 c, t dbSNP:556765660
2130 2130 c, t dbSNP:562038920
2196 2196 c, t dbSNP:769951978
2221 2221 a, t dbSNP:187516157
2224 2224 g, t dbSNP:748271362
2232 2232 c, t dbSNP:146104564
2260 2260 c, t dbSNP:34017519
2274 2274 g, t dbSNP:535297087
2277 2277 g, t dbSNP:761766986
2288 2288 a, c, g dbSNP:755230057
2289 2289 g, t dbSNP:566231195
2313 2313 c, t dbSNP:113670631
2344 2344 c, t dbSNP:12691
2354 2354 g, t dbSNP:116528776
2443 2443 a, g dbSNP:549827431
2445 2445 c, g dbSNP:576184661
2467 2467 a, c dbSNP:780450509
2478 2478 c, g dbSNP:533270582
2486 2486 c, g dbSNP:564092451
2487 2487 c, g dbSNP:547395645
2507 2507 a, g dbSNP:758844560
2543 2543 c, g dbSNP:35196724
2604 2604 a, t dbSNP:749296829

Target ORF information:

RefSeq Version NM_001287424
Organism Homo sapiens (human)
Definition Homo sapiens CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001287424

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
GTGCGCGGGC GCGGGCGAGC AGGGTCTCCG GGTGGGCGGC GGCGACGCCC CGCGCAGGCT 
GGAGGCCGCC GAGGCTCGCC ATGCCGGGAG AACTCTAACT CCCCCATGGA GTCGGCCGAC
TTCTACGAGG CGGAGCCGCG GCCCCCGATG AGCAGCCACC TGCAGAGCCC CCCGCACGCG
CCCAGCAGCG CCGCCTTCGG CTTTCCCCGG GGCGCGGGCC CCGCGCAGCC TCCCGCCCCA
CCTGCCGCCC CGGAGCCGCT GGGCGGCATC TGCGAGCACG AGACGTCCAT CGACATCAGC
GCCTACATCG ACCCGGCCGC CTTCAACGAC GAGTTCCTGG CCGACCTGTT CCAGCACAGC
CGGCAGCAGG AGAAGGCCAA GGCGGCCGTG GGCCCCACGG GCGGCGGCGG CGGCGGCGAC
TTTGACTACC CGGGCGCGCC CGCGGGCCCC GGCGGCGCCG TCATGCCCGG GGGAGCGCAC
GGGCCCCCGC CCGGCTACGG CTGCGCGGCC GCCGGCTACC TGGACGGCAG GCTGGAGCCC
CTGTACGAGC GCGTCGGGGC GCCGGCGCTG CGGCCGCTGG TGATCAAGCA GGAGCCCCGC
GAGGAGGATG AAGCCAAGCA GCTGGCGCTG GCCGGCCTCT TCCCTTACCA GCCGCCGCCG
CCGCCGCCGC CCTCGCACCC GCACCCGCAC CCGCCGCCCG CGCACCTGGC CGCCCCGCAC
CTGCAGTTCC AGATCGCGCA CTGCGGCCAG ACCACCATGC ACCTGCAGCC CGGTCACCCC
ACGCCGCCGC CCACGCCCGT GCCCAGCCCG CACCCCGCGC CCGCGCTCGG TGCCGCCGGC
CTGCCGGGCC CTGGCAGCGC GCTCAAGGGG CTGGGCGCCG CGCACCCCGA CCTCCGCGCG
AGTGGCGGCA GCGGCGCGGG CAAGGCCAAG AAGTCGGTGG ACAAGAACAG CAACGAGTAC
CGGGTGCGGC GCGAGCGCAA CAACATCGCG GTGCGCAAGA GCCGCGACAA GGCCAAGCAG
CGCAACGTGG AGACGCAGCA GAAGGTGCTG GAGCTGACCA GTGACAATGA CCGCCTGCGC
AAGCGGGTGG AACAGCTGAG CCGCGAACTG GACACGCTGC GGGGCATCTT CCGCCAGCTG
CCAGAGAGCT CCTTGGTCAA GGCCATGGGC AACTGCGCGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MicroRNA let-7a-3 gene methylation is associated with karyotyping, CEBPA promoter methylation, and survival in acute myeloid leukemia
Leuk. Res. 38 (5), 625-631 (2014)
Ko YC, Fang WH, Lin TC, Hou HA, Chen CY, Tien HF and Lin LI.


book

Nucleolar retention of a translational C/EBPalpha isoform stimulates rDNA transcription and cell size
EMBO J. 29 (5), 897-909 (2010)
Muller C, Bremer A, Schreiber S, Eichwald S and Calkhoven CF.


book

Translational control of C/EBPalpha and C/EBPbeta isoform expression
Genes Dev. 14 (15), 1920-1932 (2000)
Calkhoven CF, Muller C and Leutz A.


book

Inhibition of CCAAT/enhancer-binding protein alpha and beta translation by upstream open reading frames
J. Biol. Chem. 273 (16), 9552-9560 (1998)
Lincoln AJ, Monczak Y, Williams SC and Johnson PF.


book

Transcription factor C/EBPalpha: novel sites of expression and cloning of the human gene
Biol. Chem. 378 (5), 373-379 (1997)
Swart GW, van Groningen JJ, van Ruissen F, Bergers M and Schalkwijk J.


book

Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha)
Biochem. Biophys. Res. Commun. 215 (1), 106-113 (1995)
Antonson P and Xanthopoulos KG.


book

A 30-kDa alternative translation product of the CCAAT/enhancer binding protein alpha message: transcriptional activator lacking antimitotic activity
Proc. Natl. Acad. Sci. U.S.A. 90 (20), 9606-9610 (1993)
Lin FT, MacDougald OA, Diehl AM and Lane MD.


book

CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials
Proc. Natl. Acad. Sci. U.S.A. 90 (17), 8219-8223 (1993)
Ossipow V, Descombes P and Schibler U.


book

Familial Acute Myeloid Leukemia (AML) with Mutated CEBPA
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Klein,R.D. and Marcucci,G.


book

The CCAAT/enhancer binding protein (C/EBP alpha) gene (CEBPA) maps to human chromosome 19q13.1 and the related nuclear factor NF-IL6 (C/EBP beta) gene (CEBPB) maps to human chromosome 20q13.1
Genomics 14 (1), 12-17 (1992)
Hendricks-Taylor LR, Bachinski LL, Siciliano MJ, Fertitta A, Trask B, de Jong PJ, Ledbetter DH and Darlington GJ.


 
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