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AGPAT2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol AGPAT2
Entrez Gene ID 10555
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 2
Synonyms 1-AGPAT2, BSCL, BSCL1, LPAAB, LPAAT-beta
General protein information
Preferred Names
1-acyl-sn-glycerol-3-phosphate acyltransferase beta
Names
1-acyl-sn-glycerol-3-phosphate acyltransferase beta
1-AGPAT 2
1-AGP acyltransferase 2
lysophosphatidic acid acyltransferase beta
lysophosphatidic acid acyltransferase-beta
1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
NP_001012745.1
NP_006403.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

9

9q34.3

Summary This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

603100

Disorder Html: Lipodystrophy, congenital generalized, type 1, 608594 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001012727 NP_001012745 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform b precursor
NM_006412 NP_006403 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform a precursor



Homo sapiens (human) AGPAT2 NP_006403.2
Pan troglodytes (chimpanzee) AGPAT2 XP_001162511.3
Macaca mulatta (Rhesus monkey) AGPAT2 XP_001094500.1
Canis lupus familiaris (dog) AGPAT2 XP_548370.4
Bos taurus (cattle) AGPAT2 NP_001073733.1
Mus musculus (house mouse) Agpat2 NP_080488.1
Rattus norvegicus (Norway rat) Agpat2 NP_001101291.1
Gallus gallus (chicken) AGPAT2 XP_001235300.2
Xenopus (Silurana) tropicalis (western clawed frog) agpat2 NP_001123741.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGPAT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGPAT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu23150
NM_001012727 Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $49.50
$99.00
OHu22397
NM_006412 Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $49.50
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23150
Clone ID Related Accession (Same CDS sequence) NM_001012727
Accession Version NM_001012727.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 741bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF347518.1, CN407343.1, BC004529.2, AL590226.23 and BC000026.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004529.2, BG281349.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG
GTCAGGGAGA ACGTGCCCAT CGTCCCCGTG GTGTACTCTT CCTTCTCCTC CTTCTACAAC
ACCAAGAAGA AGTTCTTCAC TTCAGGAACA GTCACAGTGC AGGTGCTGGA AGCCATCCCC
ACCAGCGGCC TCACTGCGGC GGACGTCCCT GCGCTCGTGG ACACCTGCCA CCGGGCCATG
AGGACCACCT TCCTCCACAT CTCCAAGACC CCCCAGGAGA ACGGGGCCAC TGCGGGGTCT
GGCGTGCAGC CGGCCCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001012745.1
CDS103..843
Misc Feature(1)31..33(+)
Misc Feature(2)301..753(+)
Misc Feature(3)394..468(+)
Exon (1)1..284
Gene:AGPAT2
Gene Synonym:
Exon (2)285..418
Gene:AGPAT2
Gene Synonym:
Exon (3)419..594
Gene:AGPAT2
Gene Synonym:
Exon (4)595..667
Gene:AGPAT2
Gene Synonym:
Exon (5)668..1452
Gene:AGPAT2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001012727
Organism Homo sapiens (human)
Definition Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001012727

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG
GTCAGGGAGA ACGTGCCCAT CGTCCCCGTG GTGTACTCTT CCTTCTCCTC CTTCTACAAC
ACCAAGAAGA AGTTCTTCAC TTCAGGAACA GTCACAGTGC AGGTGCTGGA AGCCATCCCC
ACCAGCGGCC TCACTGCGGC GGACGTCCCT GCGCTCGTGG ACACCTGCCA CCGGGCCATG
AGGACCACCT TCCTCCACAT CTCCAAGACC CCCCAGGAGA ACGGGGCCAC TGCGGGGTCT
GGCGTGCAGC CGGCCCAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22397
Clone ID Related Accession (Same CDS sequence) NM_006412
Accession Version NM_006412.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 837bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF347518.1, AL590226.23, AF000237.1 and BC000026.2. This sequence is a reference standard in the RefSeqGene project. On Jul 5, 2005 this sequence version replaced gi:6041664. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF011374.1, AF000237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153980, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG
GTCAGGGAGA ACCTCAAAGT GTGGATCTAT CCCGAGGGTA CTCGCAACGA CAATGGGGAC
CTGCTGCCTT TTAAGAAGGG CGCCTTCTAC CTGGCAGTCC AGGCACAGGT GCCCATCGTC
CCCGTGGTGT ACTCTTCCTT CTCCTCCTTC TACAACACCA AGAAGAAGTT CTTCACTTCA
GGAACAGTCA CAGTGCAGGT GCTGGAAGCC ATCCCCACCA GCGGCCTCAC TGCGGCGGAC
GTCCCTGCGC TCGTGGACAC CTGCCACCGG GCCATGAGGA CCACCTTCCT CCACATCTCC
AAGACCCCCC AGGAGAACGG GGCCACTGCG GGGTCTGGCG TGCAGCCGGC CCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006403.2
CDS103..939
Misc Feature(1)31..33(+)
Misc Feature(2)190..252(+)
Misc Feature(3)301..849(+)
Misc Feature(4)394..627(+)
Misc Feature(5)394..411(+)
Misc Feature(6)466..528(+)
Misc Feature(7)616..627(+)
Misc Feature(8)661..723(+)
Exon (1)1..284
Gene:AGPAT2
Gene Synonym:
Exon (2)285..418
Gene:AGPAT2
Gene Synonym:
Exon (3)419..594
Gene:AGPAT2
Gene Synonym:
Exon (4)595..690
Gene:AGPAT2
Gene Synonym:
Exon (5)691..763
Gene:AGPAT2
Gene Synonym:
Exon (6)764..1548
Gene:AGPAT2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006412
Organism Homo sapiens (human)
Definition Homo sapiens 1-acylglycerol-3-phosphate O-acyltransferase 2 (AGPAT2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006412

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGAGCTGT GGCCGTGTCT GGCCGCGGCG CTGCTGTTGC TGCTGCTGCT GGTGCAGCTG 
AGCCGCGCGG CCGAGTTCTA CGCCAAGGTC GCCCTGTACT GCGCGCTGTG CTTCACGGTG
TCCGCCGTGG CCTCGCTCGT CTGCCTGCTG CGCCACGGCG GCCGGACGGT GGAGAACATG
AGCATCATCG GCTGGTTCGT GCGAAGCTTC AAGTACTTTT ACGGGCTCCG CTTCGAGGTG
CGGGACCCGC GCAGGCTGCA GGAGGCCCGT CCCTGTGTCA TCGTCTCCAA CCACCAGAGC
ATCCTGGACA TGATGGGCCT CATGGAGGTC CTTCCGGAGC GCTGCGTGCA GATCGCCAAG
CGGGAGCTGC TCTTCCTGGG GCCCGTGGGC CTCATCATGT ACCTCGGGGG CGTCTTCTTC
ATCAACCGGC AGCGCTCTAG CACTGCCATG ACAGTGATGG CCGACCTGGG CGAGCGCATG
GTCAGGGAGA ACCTCAAAGT GTGGATCTAT CCCGAGGGTA CTCGCAACGA CAATGGGGAC
CTGCTGCCTT TTAAGAAGGG CGCCTTCTAC CTGGCAGTCC AGGCACAGGT GCCCATCGTC
CCCGTGGTGT ACTCTTCCTT CTCCTCCTTC TACAACACCA AGAAGAAGTT CTTCACTTCA
GGAACAGTCA CAGTGCAGGT GCTGGAAGCC ATCCCCACCA GCGGCCTCAC TGCGGCGGAC
GTCCCTGCGC TCGTGGACAC CTGCCACCGG GCCATGAGGA CCACCTTCCT CCACATCTCC
AAGACCCCCC AGGAGAACGG GGCCACTGCG GGGTCTGGCG TGCAGCCGGC CCAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

MicroRNA-24 inhibits osteosarcoma cell proliferation both in vitro and in vivo by targeting LPAATbeta
Arch. Biochem. Biophys. 535 (2), 128-135 (2013)
Song L, Yang J, Duan P, Xu J, Luo X, Luo F, Zhang Z, Hou T, Liu B and Zhou Q.


book

Identification of a novel nonsense mutation and a missense substitution in the AGPAT2 gene causing congenital generalized lipodystrophy type 1
Eur J Med Genet 55 (11), 620-624 (2012)
Haghighi A, Razzaghy-Azar M, Talea A, Sadeghian M, Ellard S and Haghighi A.


book

Alterations in lipid signaling underlie lipodystrophy secondary to AGPAT2 mutations
Diabetes 61 (11), 2922-2931 (2012)
Subauste AR, Das AK, Li X, Elliott BG, Evans C, El Azzouny M, Treutelaar M, Oral E, Leff T and Burant CF.


book

Human 1-acylglycerol-3-phosphate O-acyltransferase isoforms 1 and 2: biochemical characterization and inability to rescue hepatic steatosis in Agpat2(-/-) gene lipodystrophic mice
J. Biol. Chem. 286 (43), 37676-37691 (2011)
Agarwal AK, Sukumaran S, Cortes VA, Tunison K, Mizrachi D, Sankella S, Gerard RD, Horton JD and Garg A.


book

[Lysophosphatidic acid acyltransferase beta gene expression in newly diagnosed leukemia patients]
Zhongguo Shi Yan Xue Ye Xue Za Zhi 18 (6), 1422-1426 (2010)
Zhan R, Huang HB, Wu SQ and Lin J.


book

A human cDNA sequence with homology to non-mammalian lysophosphatidic acid acyltransferases
Biochem. J. 326 (PT 2), 455-461 (1997)
Stamps AC, Elmore MA, Hill ME, Kelly K, Makda AA and Finnen MJ.


book

Human lysophosphatidic acid acyltransferase. cDNA cloning, expression, and localization to chromosome 9q34.3
J. Biol. Chem. 272 (32), 20299-20305 (1997)
Eberhardt C, Gray PW and Tjoelker LW.


book

Cloning and expression of two human lysophosphatidic acid acyltransferase cDNAs that enhance cytokine-induced signaling responses in cells
DNA Cell Biol. 16 (6), 691-701 (1997)
West J, Tompkins CK, Balantac N, Nudelman E, Meengs B, White T, Bursten S, Coleman J, Kumar A, Singer JW and Leung DW.


book

Biosynthesis of triacylglycerols
Prog. Lipid Res. 35 (2), 169-201 (1996)
Lehner R and Kuksis A.


book

Berardinelli-Seip Congenital Lipodystrophy
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Van Maldergem,L.


 
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