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CFTR cDNA ORF clone, Homo sapiens (human)

Gene Symbol CFTR
Entrez Gene ID 1080
Full Name cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Synonyms ABC35, ABCC7, CF, CFTR/MRP, MRP7, TNR-CFTR, dJ760C5.1
General protein information
Preferred Names
cystic fibrosis transmembrane conductance regulator
Names
cystic fibrosis transmembrane conductance regulator
cAMP-dependent chloride channel
channel conductance-controlling ATPase
NP_000483.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q31.2

Summary This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

602421

Disorder Html: Cystic fibrosis, 219700 (3); Congenital bilateral absence of vas

mRNA and Protein(s)

mRNA Protein Name
XM_011515751 XP_011514053 cystic fibrosis transmembrane conductance regulator isoform X1
XM_011515752 XP_011514054 cystic fibrosis transmembrane conductance regulator isoform X2
XM_011515753 XP_011514055 cystic fibrosis transmembrane conductance regulator isoform X3
XM_011515754 XP_011514056 cystic fibrosis transmembrane conductance regulator isoform X3
NM_000492 NP_000483 cystic fibrosis transmembrane conductance regulator



Homo sapiens (human) CFTR NP_000483.3
Pan troglodytes (chimpanzee) CFTR NP_001073386.1
Macaca mulatta (Rhesus monkey) CFTR NP_001028110.1
Canis lupus familiaris (dog) CFTR NP_001007144.1
Bos taurus (cattle) CFTR NP_776443.1
Mus musculus (house mouse) Cftr NP_066388.1
Rattus norvegicus (Norway rat) Cftr NP_113694.1
Gallus gallus (chicken) CFTR NP_001099136.1
Danio rerio (zebrafish) cftr NP_001038348.1
Xenopus (Silurana) tropicalis (western clawed frog) cftr XP_002935002.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CFTR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFTR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu56826 XM_011515751 PREDICTED: Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $811.30
$1159.00
OHu56827 XM_011515752 PREDICTED: Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu56828 XM_011515753 PREDICTED: Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $727.30
$1039.00
OHu56828 XM_011515754 PREDICTED: Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $727.30
$1039.00
OHu27239 NM_000492 Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $923.30
$1319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu56826
Clone ID Related Accession (Same CDS sequence) XM_011515751
Accession Version XM_011515751.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cystic fibrosis transmembrane conductance regulator isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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361
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGAAAACAC AGGTAGAGGA TTTCAAACAA CAGAGTGGAT GTACGTGTCC TAAGATTTCT 
GTGCCACCCT TGGAGTTCAC TCACCTAAAC CTGAAACTAA TAAAGCTTGG TTCTTTTCTC
CGACACGCAA AGGAAGCGCT AAGCTGGACC AGACCAATTT TGAGGAAAGG ATACAGACAG
CGCCTGGAAT TGTCAGACAT ATACCAAATC CCTTCTGTTG ATTCTGCTGA CAATCTATCT
GAAAAATTGG AAAGAGAATG GGATAGAGAG CTGGCTTCAA AGAAAAATCC TAAACTCATT
AATGCCCTTC GGCGATGTTT TTTCTGGAGA TTTATGTTCT ATGGAATCTT TTTATATTTA
GGGGAAGTCA CCAAAGCAGT ACAGCCTCTC TTACTGGGAA GAATCATAGC TTCCTATGAC
CCGGATAACA AGGAGGAACG CTCTATCGCG ATTTATCTAG GCATAGGCTT ATGCCTTCTC
TTTATTGTGA GGACACTGCT CCTACACCCA GCCATTTTTG GCCTTCATCA CATTGGAATG
CAGATGAGAA TAGCTATGTT TAGTTTGATT TATAAGAAGA CTTTAAAGCT GTCAAGCCGT
GTTCTAGATA AAATAAGTAT TGGACAACTT GTTAGTCTCC TTTCCAACAA CCTGAACAAA
TTTGATGAAG GACTTGCATT GGCACATTTC GTGTGGATCG CTCCTTTGCA AGTGGCACTC
CTCATGGGGC TAATCTGGGA GTTGTTACAG GCGTCTGCCT TCTGTGGACT TGGTTTCCTG
ATAGTCCTTG CCCTTTTTCA GGCTGGGCTA GGGAGAATGA TGATGAAGTA CAGAGATCAG
AGAGCTGGGA AGATCAGTGA AAGACTTGTG ATTACCTCAG AAATGATTGA AAATATCCAA
TCTGTTAAGG CATACTGCTG GGAAGAAGCA ATGGAAAAAA TGATTGAAAA CTTAAGACAA
ACAGAACTGA AACTGACTCG GAAGGCAGCC TATGTGAGAT ACTTCAATAG CTCAGCCTTC
TTCTTCTCAG GGTTCTTTGT GGTGTTTTTA TCTGTGCTTC CCTATGCACT AATCAAAGGA
ATCATCCTCC GGAAAATATT CACCACCATC TCATTCTGCA TTGTTCTGCG CATGGCGGTC
ACTCGGCAAT TTCCCTGGGC TGTACAAACA TGGTATGACT CTCTTGGAGC AATAAACAAA
ATACAGGATT TCTTACAAAA GCAAGAATAT AAGACATTGG AATATAACTT AACGACTACA
GAAGTAGTGA TGGAGAATGT AACAGCCTTC TGGGAGGAGG GATTTGGGGA ATTATTTGAG
AAAGCAAAAC AAAACAATAA CAATAGAAAA ACTTCTAATG GTGATGACAG CCTCTTCTTC
AGTAATTTCT CACTTCTTGG TACTCCTGTC CTGAAAGATA TTAATTTCAA GATAGAAAGA
GGACAGTTGT TGGCGGTTGC TGGATCCACT GGAGCAGGCA AGACTTCACT TCTAATGGTG
ATTATGGGAG AACTGGAGCC TTCAGAGGGT AAAATTAAGC ACAGTGGAAG AATTTCATTC
TGTTCTCAGT TTTCCTGGAT TATGCCTGGC ACCATTAAAG AAAATATCAT CTTTGGTGTT
TCCTATGATG AATATAGATA CAGAAGCGTC ATCAAAGCAT GCCAACTAGA AGAGGACATC
TCCAAGTTTG CAGAGAAAGA CAATATAGTT CTTGGAGAAG GTGGAATCAC ACTGAGTGGA
GGTCAACGAG CAAGAATTTC TTTAGCAAGA GCAGTATACA AAGATGCTGA TTTGTATTTA
TTAGACTCTC CTTTTGGATA CCTAGATGTT TTAACAGAAA AAGAAATATT TGAAAGCTGT
GTCTGTAAAC TGATGGCTAA CAAAACTAGG ATTTTGGTCA CTTCTAAAAT GGAACATTTA
AAGAAAGCTG ACAAAATATT AATTTTGCAT GAAGGTAGCA GCTATTTTTA TGGGACATTT
TCAGAACTCC AAAATCTACA GCCAGACTTT AGCTCAAAAC TCATGGGATG TGATTCTTTC
GACCAATTTA GTGCAGAAAG AAGAAATTCA ATCCTAACTG AGACCTTACA CCGTTTCTCA
TTAGAAGGAG ATGCTCCTGT CTCCTGGACA GAAACAAAAA AACAATCTTT TAAACAGACT
GGAGAGTTTG GGGAAAAAAG GAAGAATTCT ATTCTCAATC CAATCAACTC TATACGAAAA
TTTTCCATTG TGCAAAAGAC TCCCTTACAA ATGAATGGCA TCGAAGAGGA TTCTGATGAG
CCTTTAGAGA GAAGGCTGTC CTTAGTACCA GATTCTGAGC AGGGAGAGGC GATACTGCCT
CGCATCAGCG TGATCAGCAC TGGCCCCACG CTTCAGGCAC GAAGGAGGCA GTCTGTCCTG
AACCTGATGA CACACTCAGT TAACCAAGGT CAGAACATTC ACCGAAAGAC AACAGCATCC
ACACGAAAAG TGTCACTGGC CCCTCAGGCA AACTTGACTG AACTGGATAT ATATTCAAGA
AGGTTATCTC AAGAAACTGG CTTGGAAATA AGTGAAGAAA TTAACGAAGA AGACTTAAAG
GAGTGCTTTT TTGATGATAT GGAGAGCATA CCAGCAGTGA CTACATGGAA CACATACCTT
CGATATATTA CTGTCCACAA GAGCTTAATT TTTGTGCTAA TTTGGTGCTT AGTAATTTTT
CTGGCAGAGG TGGCTGCTTC TTTGGTTGTG CTGTGGCTCC TTGGAAACAC TCCTCTTCAA
GACAAAGGGA ATAGTACTCA TAGTAGAAAT AACAGCTATG CAGTGATTAT CACCAGCACC
AGTTCGTATT ATGTGTTTTA CATTTACGTG GGAGTAGCCG ACACTTTGCT TGCTATGGGA
TTCTTCAGAG GTCTACCACT GGTGCATACT CTAATCACAG TGTCGAAAAT TTTACACCAC
AAAATGTTAC ATTCTGTTCT TCAAGCACCT ATGTCAACCC TCAACACGTT GAAAGCAGGT
GGGATTCTTA ATAGATTCTC CAAAGATATA GCAATTTTGG ATGACCTTCT GCCTCTTACC
ATATTTGACT TCATCCAGTT GTTATTAATT GTGATTGGAG CTATAGCAGT TGTCGCAGTT
TTACAACCCT ACATCTTTGT TGCAACAGTG CCAGTGATAG TGGCTTTTAT TATGTTGAGA
GCATATTTCC TCCAAACCTC ACAGCAACTC AAACAACTGG AATCTGAAGG CAGGAGTCCA
ATTTTCACTC ATCTTGTTAC AAGCTTAAAA GGACTATGGA CACTTCGTGC CTTCGGACGG
CAGCCTTACT TTGAAACTCT GTTCCACAAA GCTCTGAATT TACATACTGC CAACTGGTTC
TTGTACCTGT CAACACTGCG CTGGTTCCAA ATGAGAATAG AAATGATTTT TGTCATCTTC
TTCATTGCTG TTACCTTCAT TTCCATTTTA ACAACAGGAG AAGGAGAAGG AAGAGTTGGT
ATTATCCTGA CTTTAGCCAT GAATATCATG AGTACATTGC AGTGGGCTGT AAACTCCAGC
ATAGATGTGG ATAGCTTGAT GCGATCTGTG AGCCGAGTCT TTAAGTTCAT TGACATGCCA
ACAGAAGGTA AACCTACCAA GTCAACCAAA CCATACAAGA ATGGCCAACT CTCGAAAGTT
ATGATTATTG AGAATTCACA CGTGAAGAAA GATGACATCT GGCCCTCAGG GGGCCAAATG
ACTGTCAAAG ATCTCACAGC AAAATACACA GAAGGTGGAA ATGCCATATT AGAGAACATT
TCCTTCTCAA TAAGTCCTGG CCAGAGGGTG GGCCTCTTGG GAAGAACTGG ATCAGGGAAG
AGTACTTTGT TATCAGCTTT TTTGAGACTA CTGAACACTG AAGGAGAAAT CCAGATCGAT
GGTGTGTCTT GGGATTCAAT AACTTTGCAA CAGTGGAGGA AAGCCTTTGG AGTGATACCA
CAGAAAGTAT TTATTTTTTC TGGAACATTT AGAAAAAACT TGGATCCCTA TGAACAGTGG
AGTGATCAAG AAATATGGAA AGTTGCAGAT GAGGTTGGGC TCAGATCTGT GATAGAACAG
TTTCCTGGGA AGCTTGACTT TGTCCTTGTG GATGGGGGCT GTGTCCTAAG CCATGGCCAC
AAGCAGTTGA TGTGCTTGGC TAGATCTGTT CTCAGTAAGG CGAAGATCTT GCTGCTTGAT
GAACCCAGTG CTCATTTGGA TCCAGTAACA TACCAAATAA TTAGAAGAAC TCTAAAACAA
GCATTTGCTG ATTGCACAGT AATTCTCTGT GAACACAGGA TAGAAGCAAT GCTGGAATGC
CAACAATTTT TGGTCATAGA AGAGAACAAA GTGCGGCAGT ACGATTCCAT CCAGAAACTG
CTGAACGAGA GGAGCCTCTT CCGGCAAGCC ATCAGCCCCT CCGACAGGGT GAAGCTCTTT
CCCCACCGGA ACTCAAGCAA GTGCAAGTCT AAGCCCCAGA TTGCTGCTCT GAAAGAGGAG
ACAGAAGAAG AGGTGCAAGA TACAAGGCTT TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514053.1
CDS29..4561
Misc Feature(1)155..4558(+)
Misc Feature(2)365..1168(+)
Misc Feature(3)1283..2128(+)
Misc Feature(4)1490..1513(+)
Misc Feature(5)1499..1933(+)
Misc Feature(6)1586..1597(+)
Misc Feature(7)1760..1789(+)
Misc Feature(8)1820..1837(+)
Misc Feature(9)1844..1855(+)
Misc Feature(10)1919..1939(+)
Misc Feature(11)2033..2668(+)
Misc Feature(12)2702..3559(+)
Misc Feature(13)3740..4558(+)
Misc Feature(14)3848..3871(+)
Misc Feature(15)3857..4324(+)
Misc Feature(16)3980..3991(+)
Misc Feature(17)4154..4183(+)
Misc Feature(18)4214..4231(+)
Misc Feature(19)4238..4249(+)
Misc Feature(20)4310..4330(+)
Translation

Target ORF information:

RefSeq Version XM_011515751
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515751

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGAAAACAC AGGTAGAGGA TTTCAAACAA CAGAGTGGAT GTACGTGTCC TAAGATTTCT 
GTGCCACCCT TGGAGTTCAC TCACCTAAAC CTGAAACTAA TAAAGCTTGG TTCTTTTCTC
CGACACGCAA AGGAAGCGCT AAGCTGGACC AGACCAATTT TGAGGAAAGG ATACAGACAG
CGCCTGGAAT TGTCAGACAT ATACCAAATC CCTTCTGTTG ATTCTGCTGA CAATCTATCT
GAAAAATTGG AAAGAGAATG GGATAGAGAG CTGGCTTCAA AGAAAAATCC TAAACTCATT
AATGCCCTTC GGCGATGTTT TTTCTGGAGA TTTATGTTCT ATGGAATCTT TTTATATTTA
GGGGAAGTCA CCAAAGCAGT ACAGCCTCTC TTACTGGGAA GAATCATAGC TTCCTATGAC
CCGGATAACA AGGAGGAACG CTCTATCGCG ATTTATCTAG GCATAGGCTT ATGCCTTCTC
TTTATTGTGA GGACACTGCT CCTACACCCA GCCATTTTTG GCCTTCATCA CATTGGAATG
CAGATGAGAA TAGCTATGTT TAGTTTGATT TATAAGAAGA CTTTAAAGCT GTCAAGCCGT
GTTCTAGATA AAATAAGTAT TGGACAACTT GTTAGTCTCC TTTCCAACAA CCTGAACAAA
TTTGATGAAG GACTTGCATT GGCACATTTC GTGTGGATCG CTCCTTTGCA AGTGGCACTC
CTCATGGGGC TAATCTGGGA GTTGTTACAG GCGTCTGCCT TCTGTGGACT TGGTTTCCTG
ATAGTCCTTG CCCTTTTTCA GGCTGGGCTA GGGAGAATGA TGATGAAGTA CAGAGATCAG
AGAGCTGGGA AGATCAGTGA AAGACTTGTG ATTACCTCAG AAATGATTGA AAATATCCAA
TCTGTTAAGG CATACTGCTG GGAAGAAGCA ATGGAAAAAA TGATTGAAAA CTTAAGACAA
ACAGAACTGA AACTGACTCG GAAGGCAGCC TATGTGAGAT ACTTCAATAG CTCAGCCTTC
TTCTTCTCAG GGTTCTTTGT GGTGTTTTTA TCTGTGCTTC CCTATGCACT AATCAAAGGA
ATCATCCTCC GGAAAATATT CACCACCATC TCATTCTGCA TTGTTCTGCG CATGGCGGTC
ACTCGGCAAT TTCCCTGGGC TGTACAAACA TGGTATGACT CTCTTGGAGC AATAAACAAA
ATACAGGATT TCTTACAAAA GCAAGAATAT AAGACATTGG AATATAACTT AACGACTACA
GAAGTAGTGA TGGAGAATGT AACAGCCTTC TGGGAGGAGG GATTTGGGGA ATTATTTGAG
AAAGCAAAAC AAAACAATAA CAATAGAAAA ACTTCTAATG GTGATGACAG CCTCTTCTTC
AGTAATTTCT CACTTCTTGG TACTCCTGTC CTGAAAGATA TTAATTTCAA GATAGAAAGA
GGACAGTTGT TGGCGGTTGC TGGATCCACT GGAGCAGGCA AGACTTCACT TCTAATGGTG
ATTATGGGAG AACTGGAGCC TTCAGAGGGT AAAATTAAGC ACAGTGGAAG AATTTCATTC
TGTTCTCAGT TTTCCTGGAT TATGCCTGGC ACCATTAAAG AAAATATCAT CTTTGGTGTT
TCCTATGATG AATATAGATA CAGAAGCGTC ATCAAAGCAT GCCAACTAGA AGAGGACATC
TCCAAGTTTG CAGAGAAAGA CAATATAGTT CTTGGAGAAG GTGGAATCAC ACTGAGTGGA
GGTCAACGAG CAAGAATTTC TTTAGCAAGA GCAGTATACA AAGATGCTGA TTTGTATTTA
TTAGACTCTC CTTTTGGATA CCTAGATGTT TTAACAGAAA AAGAAATATT TGAAAGCTGT
GTCTGTAAAC TGATGGCTAA CAAAACTAGG ATTTTGGTCA CTTCTAAAAT GGAACATTTA
AAGAAAGCTG ACAAAATATT AATTTTGCAT GAAGGTAGCA GCTATTTTTA TGGGACATTT
TCAGAACTCC AAAATCTACA GCCAGACTTT AGCTCAAAAC TCATGGGATG TGATTCTTTC
GACCAATTTA GTGCAGAAAG AAGAAATTCA ATCCTAACTG AGACCTTACA CCGTTTCTCA
TTAGAAGGAG ATGCTCCTGT CTCCTGGACA GAAACAAAAA AACAATCTTT TAAACAGACT
GGAGAGTTTG GGGAAAAAAG GAAGAATTCT ATTCTCAATC CAATCAACTC TATACGAAAA
TTTTCCATTG TGCAAAAGAC TCCCTTACAA ATGAATGGCA TCGAAGAGGA TTCTGATGAG
CCTTTAGAGA GAAGGCTGTC CTTAGTACCA GATTCTGAGC AGGGAGAGGC GATACTGCCT
CGCATCAGCG TGATCAGCAC TGGCCCCACG CTTCAGGCAC GAAGGAGGCA GTCTGTCCTG
AACCTGATGA CACACTCAGT TAACCAAGGT CAGAACATTC ACCGAAAGAC AACAGCATCC
ACACGAAAAG TGTCACTGGC CCCTCAGGCA AACTTGACTG AACTGGATAT ATATTCAAGA
AGGTTATCTC AAGAAACTGG CTTGGAAATA AGTGAAGAAA TTAACGAAGA AGACTTAAAG
GAGTGCTTTT TTGATGATAT GGAGAGCATA CCAGCAGTGA CTACATGGAA CACATACCTT
CGATATATTA CTGTCCACAA GAGCTTAATT TTTGTGCTAA TTTGGTGCTT AGTAATTTTT
CTGGCAGAGG TGGCTGCTTC TTTGGTTGTG CTGTGGCTCC TTGGAAACAC TCCTCTTCAA
GACAAAGGGA ATAGTACTCA TAGTAGAAAT AACAGCTATG CAGTGATTAT CACCAGCACC
AGTTCGTATT ATGTGTTTTA CATTTACGTG GGAGTAGCCG ACACTTTGCT TGCTATGGGA
TTCTTCAGAG GTCTACCACT GGTGCATACT CTAATCACAG TGTCGAAAAT TTTACACCAC
AAAATGTTAC ATTCTGTTCT TCAAGCACCT ATGTCAACCC TCAACACGTT GAAAGCAGGT
GGGATTCTTA ATAGATTCTC CAAAGATATA GCAATTTTGG ATGACCTTCT GCCTCTTACC
ATATTTGACT TCATCCAGTT GTTATTAATT GTGATTGGAG CTATAGCAGT TGTCGCAGTT
TTACAACCCT ACATCTTTGT TGCAACAGTG CCAGTGATAG TGGCTTTTAT TATGTTGAGA
GCATATTTCC TCCAAACCTC ACAGCAACTC AAACAACTGG AATCTGAAGG CAGGAGTCCA
ATTTTCACTC ATCTTGTTAC AAGCTTAAAA GGACTATGGA CACTTCGTGC CTTCGGACGG
CAGCCTTACT TTGAAACTCT GTTCCACAAA GCTCTGAATT TACATACTGC CAACTGGTTC
TTGTACCTGT CAACACTGCG CTGGTTCCAA ATGAGAATAG AAATGATTTT TGTCATCTTC
TTCATTGCTG TTACCTTCAT TTCCATTTTA ACAACAGGAG AAGGAGAAGG AAGAGTTGGT
ATTATCCTGA CTTTAGCCAT GAATATCATG AGTACATTGC AGTGGGCTGT AAACTCCAGC
ATAGATGTGG ATAGCTTGAT GCGATCTGTG AGCCGAGTCT TTAAGTTCAT TGACATGCCA
ACAGAAGGTA AACCTACCAA GTCAACCAAA CCATACAAGA ATGGCCAACT CTCGAAAGTT
ATGATTATTG AGAATTCACA CGTGAAGAAA GATGACATCT GGCCCTCAGG GGGCCAAATG
ACTGTCAAAG ATCTCACAGC AAAATACACA GAAGGTGGAA ATGCCATATT AGAGAACATT
TCCTTCTCAA TAAGTCCTGG CCAGAGGGTG GGCCTCTTGG GAAGAACTGG ATCAGGGAAG
AGTACTTTGT TATCAGCTTT TTTGAGACTA CTGAACACTG AAGGAGAAAT CCAGATCGAT
GGTGTGTCTT GGGATTCAAT AACTTTGCAA CAGTGGAGGA AAGCCTTTGG AGTGATACCA
CAGAAAGTAT TTATTTTTTC TGGAACATTT AGAAAAAACT TGGATCCCTA TGAACAGTGG
AGTGATCAAG AAATATGGAA AGTTGCAGAT GAGGTTGGGC TCAGATCTGT GATAGAACAG
TTTCCTGGGA AGCTTGACTT TGTCCTTGTG GATGGGGGCT GTGTCCTAAG CCATGGCCAC
AAGCAGTTGA TGTGCTTGGC TAGATCTGTT CTCAGTAAGG CGAAGATCTT GCTGCTTGAT
GAACCCAGTG CTCATTTGGA TCCAGTAACA TACCAAATAA TTAGAAGAAC TCTAAAACAA
GCATTTGCTG ATTGCACAGT AATTCTCTGT GAACACAGGA TAGAAGCAAT GCTGGAATGC
CAACAATTTT TGGTCATAGA AGAGAACAAA GTGCGGCAGT ACGATTCCAT CCAGAAACTG
CTGAACGAGA GGAGCCTCTT CCGGCAAGCC ATCAGCCCCT CCGACAGGGT GAAGCTCTTT
CCCCACCGGA ACTCAAGCAA GTGCAAGTCT AAGCCCCAGA TTGCTGCTCT GAAAGAGGAG
ACAGAAGAAG AGGTGCAAGA TACAAGGCTT TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56827
Clone ID Related Accession (Same CDS sequence) XM_011515752
Accession Version XM_011515752.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cystic fibrosis transmembrane conductance regulator isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAAACAC AGGTAGAGGA TTTCAAACAA CAGAGTGGAT GTACGTGTCC TAAGATTTCT 
GTGCCACCCT TGGAGTTCAC TCACCTAAAC CTGAAACTAA TAAAGCTTGG TTCTTTTCTC
CGACACGCAA AGGAAGCGCT AAGCTGGACC AGACCAATTT TGAGGAAAGG ATACAGACAG
CGCCTGGAAT TGTCAGACAT ATACCAAATC CCTTCTGTTG ATTCTGCTGA CAATCTATCT
GAAAAATTGG AAAGAGAATG GGATAGAGAG CTGGCTTCAA AGAAAAATCC TAAACTCATT
AATGCCCTTC GGCGATGTTT TTTCTGGAGA TTTATGTTCT ATGGAATCTT TTTATATTTA
GGGGAAGTCA CCAAAGCAGT ACAGCCTCTC TTACTGGGAA GAATCATAGC TTCCTATGAC
CCGGATAACA AGGAGGAACG CTCTATCGCG ATTTATCTAG GCATAGGCTT ATGCCTTCTC
TTTATTGTGA GGACACTGCT CCTACACCCA GCCATTTTTG GCCTTCATCA CATTGGAATG
CAGATGAGAA TAGCTATGTT TAGTTTGATT TATAAGAAGA CTTTAAAGCT GTCAAGCCGT
GTTCTAGATA AAATAAGTAT TGGACAACTT GTTAGTCTCC TTTCCAACAA CCTGAACAAA
TTTGATGAAG GACTTGCATT GGCACATTTC GTGTGGATCG CTCCTTTGCA AGTGGCACTC
CTCATGGGGC TAATCTGGGA GTTGTTACAG GCGTCTGCCT TCTGTGGACT TGGTTTCCTG
ATAGTCCTTG CCCTTTTTCA GGCTGGGCTA GGGAGAATGA TGATGAAGTA CAGAGATCAG
AGAGCTGGGA AGATCAGTGA AAGACTTGTG ATTACCTCAG AAATGATTGA AAATATCCAA
TCTGTTAAGG CATACTGCTG GGAAGAAGCA ATGGAAAAAA TGATTGAAAA CTTAAGACAA
ACAGAACTGA AACTGACTCG GAAGGCAGCC TATGTGAGAT ACTTCAATAG CTCAGCCTTC
TTCTTCTCAG GGTTCTTTGT GGTGTTTTTA TCTGTGCTTC CCTATGCACT AATCAAAGGA
ATCATCCTCC GGAAAATATT CACCACCATC TCATTCTGCA TTGTTCTGCG CATGGCGGTC
ACTCGGCAAT TTCCCTGGGC TGTACAAACA TGGTATGACT CTCTTGGAGC AATAAACAAA
ATACAGGATT TCTTACAAAA GCAAGAATAT AAGACATTGG AATATAACTT AACGACTACA
GAAGTAGTGA TGGAGAATGT AACAGCCTTC TGGGAGGAGG GATTTGGGGA ATTATTTGAG
AAAGCAAAAC AAAACAATAA CAATAGAAAA ACTTCTAATG GTGATGACAG CCTCTTCTTC
AGTAATTTCT CACTTCTTGG TACTCCTGTC CTGAAAGATA TTAATTTCAA GATAGAAAGA
GGACAGTTGT TGGCGGTTGC TGGATCCACT GGAGCAGGCA AGACTTCACT TCTAATGGTG
ATTATGGGAG AACTGGAGCC TTCAGAGGGT AAAATTAAGC ACAGTGGAAG AATTTCATTC
TGTTCTCAGT TTTCCTGGAT TATGCCTGGC ACCATTAAAG AAAATATCAT CTTTGGTGTT
TCCTATGATG AATATAGATA CAGAAGCGTC ATCAAAGCAT GCCAACTAGA AGAGGACATC
TCCAAGTTTG CAGAGAAAGA CAATATAGTT CTTGGAGAAG GTGGAATCAC ACTGAGTGGA
GGTCAACGAG CAAGAATTTC TTTAGCAAGA GCAGTATACA AAGATGCTGA TTTGTATTTA
TTAGACTCTC CTTTTGGATA CCTAGATGTT TTAACAGAAA AAGAAATATT TGAAAGCTGT
GTCTGTAAAC TGATGGCTAA CAAAACTAGG ATTTTGGTCA CTTCTAAAAT GGAACATTTA
AAGAAAGCTG ACAAAATATT AATTTTGCAT GAAGGTAGCA GCTATTTTTA TGGGACATTT
TCAGAACTCC AAAATCTACA GCCAGACTTT AGCTCAAAAC TCATGGGATG TGATTCTTTC
GACCAATTTA GTGCAGAAAG AAGAAATTCA ATCCTAACTG AGACCTTACA CCGTTTCTCA
TTAGAAGGAG ATGCTCCTGT CTCCTGGACA GAAACAAAAA AACAATCTTT TAAACAGACT
GGAGAGTTTG GGGAAAAAAG GAAGAATTCT ATTCTCAATC CAATCAACTC TATACGAAAA
TTTTCCATTG TGCAAAAGAC TCCCTTACAA ATGAATGGCA TCGAAGAGGA TTCTGATGAG
CCTTTAGAGA GAAGGCTGTC CTTAGTACCA GATTCTGAGC AGGGAGAGGC GATACTGCCT
CGCATCAGCG TGATCAGCAC TGGCCCCACG CTTCAGGCAC GAAGGAGGCA GTCTGTCCTG
AACCTGATGA CACACTCAGT TAACCAAGGT CAGAACATTC ACCGAAAGAC AACAGCATCC
ACACGAAAAG TGTCACTGGC CCCTCAGGCA AACTTGACTG AACTGGATAT ATATTCAAGA
AGGTTATCTC AAGAAACTGG CTTGGAAATA AGTGAAGAAA TTAACGAAGA AGACTTAAAG
GAGTGCTTTT TTGATGATAT GGAGAGCATA CCAGCAGTGA CTACATGGAA CACATACCTT
CGATATATTA CTGTCCACAA GAGCTTAATT TTTGTGCTAA TTTGGTGCTT AGTAATTTTT
CTGGCAGAGG TGGCTGCTTC TTTGGTTGTG CTGTGGCTCC TTGGAAACAC TCCTCTTCAA
GACAAAGGGA ATAGTACTCA TAGTAGAAAT AACAGCTATG CAGTGATTAT CACCAGCACC
AGTTCGTATT ATGTGTTTTA CATTTACGTG GGAGTAGCCG ACACTTTGCT TGCTATGGGA
TTCTTCAGAG GTCTACCACT GGTGCATACT CTAATCACAG TGTCGAAAAT TTTACACCAC
AAAATGTTAC ATTCTGTTCT TCAAGCACCT ATGTCAACCC TCAACACGTT GAAAGCAGGT
GGGATTCTTA ATAGATTCTC CAAAGATATA GCAATTTTGG ATGACCTTCT GCCTCTTACC
ATATTTGACT TCATCCAGTT GTTATTAATT GTGATTGGAG CTATAGCAGT TGTCGCAGTT
TTACAACCCT ACATCTTTGT TGCAACAGTG CCAGTGATAG TGGCTTTTAT TATGTTGAGA
GCATATTTCC TCCAAACCTC ACAGCAACTC AAACAACTGG AATCTGAAGG CAGGAGTCCA
ATTTTCACTC ATCTTGTTAC AAGCTTAAAA GGACTATGGA CACTTCGTGC CTTCGGACGG
CAGCCTTACT TTGAAACTCT GTTCCACAAA GCTCTGAATT TACATACTGC CAACTGGTTC
TTGTACCTGT CAACACTGCG CTGGTTCCAA ATGAGAATAG AAATGATTTT TGTCATCTTC
TTCATTGCTG TTACCTTCAT TTCCATTTTA ACAACAGGAG AAGGAGAAGG AAGAGTTGGT
ATTATCCTGA CTTTAGCCAT GAATATCATG AGTACATTGC AGTGGGCTGT AAACTCCAGC
ATAGATGTGG ATAGCTTGAT GCGATCTGTG AGCCGAGTCT TTAAGTTCAT TGACATGCCA
ACAGAAGGTA AACCTACCAA GTCAACCAAA CCATACAAGA ATGGCCAACT CTCGAAAGTT
ATGATTATTG AGAATTCACA CGTGAAGAAA GATGACATCT GGCCCTCAGG GGGCCAAATG
ACTGTCAAAG ATCTCACAGC AAAATACACA GAAGGTGGAA ATGCCATATT AGAGAACATT
TCCTTCTCAA TAAGTCCTGG CCAGAGGGTG GGCCTCTTGG GAAGAACTGG ATCAGGGAAG
AGTACTTTGT TATCAGCTTT TTTGAGACTA CTGAACACTG AAGGAGAAAT CCAGATCGAT
GGTGTGTCTT GGGATTCAAT AACTTTGCAA CAGTGGAGGA AAGCCTTTGG AGTGATACCA
CAGAAAGTAT TTATTTTTTC TGGAACATTT AGAAAAAACT TGGATCCCTA TGAACAGTGG
AGTGATCAAG AAATATGGAA AGTTGCAGAT GAGGTTGGGC TCAGATCTGT GATAGAACAG
TTTCCTGGGA AGCTTGACTT TGTCCTTGTG GATGGGGGCT GTGTCCTAAG CCATGGCCAC
AAGCAGTTGA TGTGCTTGGC TAGATCTGTT CTCAGTAAGG CGAAGATCTT GCTGCTTGAT
GAACCCAGTG CTCATTTGGA TCCAGTAACA TACCAAATAA TTAGAAGAAC TCTAAAACAA
GCATTTGCTG ATTGCACAGT AATTCTCTGT GAACACAGGA TAGAAGCAAT GCTGGAATGC
CAACAATTTT TGCAACTTCA GTTCTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514054.1
CDS43..4392
Misc Feature(1)169..4383(+)
Misc Feature(2)379..1182(+)
Misc Feature(3)1297..2142(+)
Misc Feature(4)1504..1527(+)
Misc Feature(5)1513..1947(+)
Misc Feature(6)1600..1611(+)
Misc Feature(7)1774..1803(+)
Misc Feature(8)1834..1851(+)
Misc Feature(9)1858..1869(+)
Misc Feature(10)1933..1953(+)
Misc Feature(11)2047..2682(+)
Misc Feature(12)2716..3573(+)
Misc Feature(13)3754..>4383(+)
Misc Feature(14)3862..3885(+)
Misc Feature(15)3871..4347(+)
Misc Feature(16)3994..4005(+)
Misc Feature(17)4168..4197(+)
Misc Feature(18)4228..4245(+)
Misc Feature(19)4252..4263(+)
Misc Feature(20)4333..4353(+)
Translation

Target ORF information:

RefSeq Version XM_011515752
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515752

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
ATGAAAACAC AGGTAGAGGA TTTCAAACAA CAGAGTGGAT GTACGTGTCC TAAGATTTCT 
GTGCCACCCT TGGAGTTCAC TCACCTAAAC CTGAAACTAA TAAAGCTTGG TTCTTTTCTC
CGACACGCAA AGGAAGCGCT AAGCTGGACC AGACCAATTT TGAGGAAAGG ATACAGACAG
CGCCTGGAAT TGTCAGACAT ATACCAAATC CCTTCTGTTG ATTCTGCTGA CAATCTATCT
GAAAAATTGG AAAGAGAATG GGATAGAGAG CTGGCTTCAA AGAAAAATCC TAAACTCATT
AATGCCCTTC GGCGATGTTT TTTCTGGAGA TTTATGTTCT ATGGAATCTT TTTATATTTA
GGGGAAGTCA CCAAAGCAGT ACAGCCTCTC TTACTGGGAA GAATCATAGC TTCCTATGAC
CCGGATAACA AGGAGGAACG CTCTATCGCG ATTTATCTAG GCATAGGCTT ATGCCTTCTC
TTTATTGTGA GGACACTGCT CCTACACCCA GCCATTTTTG GCCTTCATCA CATTGGAATG
CAGATGAGAA TAGCTATGTT TAGTTTGATT TATAAGAAGA CTTTAAAGCT GTCAAGCCGT
GTTCTAGATA AAATAAGTAT TGGACAACTT GTTAGTCTCC TTTCCAACAA CCTGAACAAA
TTTGATGAAG GACTTGCATT GGCACATTTC GTGTGGATCG CTCCTTTGCA AGTGGCACTC
CTCATGGGGC TAATCTGGGA GTTGTTACAG GCGTCTGCCT TCTGTGGACT TGGTTTCCTG
ATAGTCCTTG CCCTTTTTCA GGCTGGGCTA GGGAGAATGA TGATGAAGTA CAGAGATCAG
AGAGCTGGGA AGATCAGTGA AAGACTTGTG ATTACCTCAG AAATGATTGA AAATATCCAA
TCTGTTAAGG CATACTGCTG GGAAGAAGCA ATGGAAAAAA TGATTGAAAA CTTAAGACAA
ACAGAACTGA AACTGACTCG GAAGGCAGCC TATGTGAGAT ACTTCAATAG CTCAGCCTTC
TTCTTCTCAG GGTTCTTTGT GGTGTTTTTA TCTGTGCTTC CCTATGCACT AATCAAAGGA
ATCATCCTCC GGAAAATATT CACCACCATC TCATTCTGCA TTGTTCTGCG CATGGCGGTC
ACTCGGCAAT TTCCCTGGGC TGTACAAACA TGGTATGACT CTCTTGGAGC AATAAACAAA
ATACAGGATT TCTTACAAAA GCAAGAATAT AAGACATTGG AATATAACTT AACGACTACA
GAAGTAGTGA TGGAGAATGT AACAGCCTTC TGGGAGGAGG GATTTGGGGA ATTATTTGAG
AAAGCAAAAC AAAACAATAA CAATAGAAAA ACTTCTAATG GTGATGACAG CCTCTTCTTC
AGTAATTTCT CACTTCTTGG TACTCCTGTC CTGAAAGATA TTAATTTCAA GATAGAAAGA
GGACAGTTGT TGGCGGTTGC TGGATCCACT GGAGCAGGCA AGACTTCACT TCTAATGGTG
ATTATGGGAG AACTGGAGCC TTCAGAGGGT AAAATTAAGC ACAGTGGAAG AATTTCATTC
TGTTCTCAGT TTTCCTGGAT TATGCCTGGC ACCATTAAAG AAAATATCAT CTTTGGTGTT
TCCTATGATG AATATAGATA CAGAAGCGTC ATCAAAGCAT GCCAACTAGA AGAGGACATC
TCCAAGTTTG CAGAGAAAGA CAATATAGTT CTTGGAGAAG GTGGAATCAC ACTGAGTGGA
GGTCAACGAG CAAGAATTTC TTTAGCAAGA GCAGTATACA AAGATGCTGA TTTGTATTTA
TTAGACTCTC CTTTTGGATA CCTAGATGTT TTAACAGAAA AAGAAATATT TGAAAGCTGT
GTCTGTAAAC TGATGGCTAA CAAAACTAGG ATTTTGGTCA CTTCTAAAAT GGAACATTTA
AAGAAAGCTG ACAAAATATT AATTTTGCAT GAAGGTAGCA GCTATTTTTA TGGGACATTT
TCAGAACTCC AAAATCTACA GCCAGACTTT AGCTCAAAAC TCATGGGATG TGATTCTTTC
GACCAATTTA GTGCAGAAAG AAGAAATTCA ATCCTAACTG AGACCTTACA CCGTTTCTCA
TTAGAAGGAG ATGCTCCTGT CTCCTGGACA GAAACAAAAA AACAATCTTT TAAACAGACT
GGAGAGTTTG GGGAAAAAAG GAAGAATTCT ATTCTCAATC CAATCAACTC TATACGAAAA
TTTTCCATTG TGCAAAAGAC TCCCTTACAA ATGAATGGCA TCGAAGAGGA TTCTGATGAG
CCTTTAGAGA GAAGGCTGTC CTTAGTACCA GATTCTGAGC AGGGAGAGGC GATACTGCCT
CGCATCAGCG TGATCAGCAC TGGCCCCACG CTTCAGGCAC GAAGGAGGCA GTCTGTCCTG
AACCTGATGA CACACTCAGT TAACCAAGGT CAGAACATTC ACCGAAAGAC AACAGCATCC
ACACGAAAAG TGTCACTGGC CCCTCAGGCA AACTTGACTG AACTGGATAT ATATTCAAGA
AGGTTATCTC AAGAAACTGG CTTGGAAATA AGTGAAGAAA TTAACGAAGA AGACTTAAAG
GAGTGCTTTT TTGATGATAT GGAGAGCATA CCAGCAGTGA CTACATGGAA CACATACCTT
CGATATATTA CTGTCCACAA GAGCTTAATT TTTGTGCTAA TTTGGTGCTT AGTAATTTTT
CTGGCAGAGG TGGCTGCTTC TTTGGTTGTG CTGTGGCTCC TTGGAAACAC TCCTCTTCAA
GACAAAGGGA ATAGTACTCA TAGTAGAAAT AACAGCTATG CAGTGATTAT CACCAGCACC
AGTTCGTATT ATGTGTTTTA CATTTACGTG GGAGTAGCCG ACACTTTGCT TGCTATGGGA
TTCTTCAGAG GTCTACCACT GGTGCATACT CTAATCACAG TGTCGAAAAT TTTACACCAC
AAAATGTTAC ATTCTGTTCT TCAAGCACCT ATGTCAACCC TCAACACGTT GAAAGCAGGT
GGGATTCTTA ATAGATTCTC CAAAGATATA GCAATTTTGG ATGACCTTCT GCCTCTTACC
ATATTTGACT TCATCCAGTT GTTATTAATT GTGATTGGAG CTATAGCAGT TGTCGCAGTT
TTACAACCCT ACATCTTTGT TGCAACAGTG CCAGTGATAG TGGCTTTTAT TATGTTGAGA
GCATATTTCC TCCAAACCTC ACAGCAACTC AAACAACTGG AATCTGAAGG CAGGAGTCCA
ATTTTCACTC ATCTTGTTAC AAGCTTAAAA GGACTATGGA CACTTCGTGC CTTCGGACGG
CAGCCTTACT TTGAAACTCT GTTCCACAAA GCTCTGAATT TACATACTGC CAACTGGTTC
TTGTACCTGT CAACACTGCG CTGGTTCCAA ATGAGAATAG AAATGATTTT TGTCATCTTC
TTCATTGCTG TTACCTTCAT TTCCATTTTA ACAACAGGAG AAGGAGAAGG AAGAGTTGGT
ATTATCCTGA CTTTAGCCAT GAATATCATG AGTACATTGC AGTGGGCTGT AAACTCCAGC
ATAGATGTGG ATAGCTTGAT GCGATCTGTG AGCCGAGTCT TTAAGTTCAT TGACATGCCA
ACAGAAGGTA AACCTACCAA GTCAACCAAA CCATACAAGA ATGGCCAACT CTCGAAAGTT
ATGATTATTG AGAATTCACA CGTGAAGAAA GATGACATCT GGCCCTCAGG GGGCCAAATG
ACTGTCAAAG ATCTCACAGC AAAATACACA GAAGGTGGAA ATGCCATATT AGAGAACATT
TCCTTCTCAA TAAGTCCTGG CCAGAGGGTG GGCCTCTTGG GAAGAACTGG ATCAGGGAAG
AGTACTTTGT TATCAGCTTT TTTGAGACTA CTGAACACTG AAGGAGAAAT CCAGATCGAT
GGTGTGTCTT GGGATTCAAT AACTTTGCAA CAGTGGAGGA AAGCCTTTGG AGTGATACCA
CAGAAAGTAT TTATTTTTTC TGGAACATTT AGAAAAAACT TGGATCCCTA TGAACAGTGG
AGTGATCAAG AAATATGGAA AGTTGCAGAT GAGGTTGGGC TCAGATCTGT GATAGAACAG
TTTCCTGGGA AGCTTGACTT TGTCCTTGTG GATGGGGGCT GTGTCCTAAG CCATGGCCAC
AAGCAGTTGA TGTGCTTGGC TAGATCTGTT CTCAGTAAGG CGAAGATCTT GCTGCTTGAT
GAACCCAGTG CTCATTTGGA TCCAGTAACA TACCAAATAA TTAGAAGAAC TCTAAAACAA
GCATTTGCTG ATTGCACAGT AATTCTCTGT GAACACAGGA TAGAAGCAAT GCTGGAATGC
CAACAATTTT TGCAACTTCA GTTCTACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56828
Clone ID Related Accession (Same CDS sequence) XM_011515753 , XM_011515754
Accession Version XM_011515753.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cystic fibrosis transmembrane conductance regulator isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGTTCTATG GAATCTTTTT ATATTTAGGG GAAGTCACCA AAGCAGTACA GCCTCTCTTA 
CTGGGAAGAA TCATAGCTTC CTATGACCCG GATAACAAGG AGGAACGCTC TATCGCGATT
TATCTAGGCA TAGGCTTATG CCTTCTCTTT ATTGTGAGGA CACTGCTCCT ACACCCAGCC
ATTTTTGGCC TTCATCACAT TGGAATGCAG ATGAGAATAG CTATGTTTAG TTTGATTTAT
AAGAAGACTT TAAAGCTGTC AAGCCGTGTT CTAGATAAAA TAAGTATTGG ACAACTTGTT
AGTCTCCTTT CCAACAACCT GAACAAATTT GATGAAGGAC TTGCATTGGC ACATTTCGTG
TGGATCGCTC CTTTGCAAGT GGCACTCCTC ATGGGGCTAA TCTGGGAGTT GTTACAGGCG
TCTGCCTTCT GTGGACTTGG TTTCCTGATA GTCCTTGCCC TTTTTCAGGC TGGGCTAGGG
AGAATGATGA TGAAGTACAG AGATCAGAGA GCTGGGAAGA TCAGTGAAAG ACTTGTGATT
ACCTCAGAAA TGATTGAAAA TATCCAATCT GTTAAGGCAT ACTGCTGGGA AGAAGCAATG
GAAAAAATGA TTGAAAACTT AAGACAAACA GAACTGAAAC TGACTCGGAA GGCAGCCTAT
GTGAGATACT TCAATAGCTC AGCCTTCTTC TTCTCAGGGT TCTTTGTGGT GTTTTTATCT
GTGCTTCCCT ATGCACTAAT CAAAGGAATC ATCCTCCGGA AAATATTCAC CACCATCTCA
TTCTGCATTG TTCTGCGCAT GGCGGTCACT CGGCAATTTC CCTGGGCTGT ACAAACATGG
TATGACTCTC TTGGAGCAAT AAACAAAATA CAGGATTTCT TACAAAAGCA AGAATATAAG
ACATTGGAAT ATAACTTAAC GACTACAGAA GTAGTGATGG AGAATGTAAC AGCCTTCTGG
GAGGAGGGAT TTGGGGAATT ATTTGAGAAA GCAAAACAAA ACAATAACAA TAGAAAAACT
TCTAATGGTG ATGACAGCCT CTTCTTCAGT AATTTCTCAC TTCTTGGTAC TCCTGTCCTG
AAAGATATTA ATTTCAAGAT AGAAAGAGGA CAGTTGTTGG CGGTTGCTGG ATCCACTGGA
GCAGGCAAGA CTTCACTTCT AATGGTGATT ATGGGAGAAC TGGAGCCTTC AGAGGGTAAA
ATTAAGCACA GTGGAAGAAT TTCATTCTGT TCTCAGTTTT CCTGGATTAT GCCTGGCACC
ATTAAAGAAA ATATCATCTT TGGTGTTTCC TATGATGAAT ATAGATACAG AAGCGTCATC
AAAGCATGCC AACTAGAAGA GGACATCTCC AAGTTTGCAG AGAAAGACAA TATAGTTCTT
GGAGAAGGTG GAATCACACT GAGTGGAGGT CAACGAGCAA GAATTTCTTT AGCAAGAGCA
GTATACAAAG ATGCTGATTT GTATTTATTA GACTCTCCTT TTGGATACCT AGATGTTTTA
ACAGAAAAAG AAATATTTGA AAGCTGTGTC TGTAAACTGA TGGCTAACAA AACTAGGATT
TTGGTCACTT CTAAAATGGA ACATTTAAAG AAAGCTGACA AAATATTAAT TTTGCATGAA
GGTAGCAGCT ATTTTTATGG GACATTTTCA GAACTCCAAA ATCTACAGCC AGACTTTAGC
TCAAAACTCA TGGGATGTGA TTCTTTCGAC CAATTTAGTG CAGAAAGAAG AAATTCAATC
CTAACTGAGA CCTTACACCG TTTCTCATTA GAAGGAGATG CTCCTGTCTC CTGGACAGAA
ACAAAAAAAC AATCTTTTAA ACAGACTGGA GAGTTTGGGG AAAAAAGGAA GAATTCTATT
CTCAATCCAA TCAACTCTAT ACGAAAATTT TCCATTGTGC AAAAGACTCC CTTACAAATG
AATGGCATCG AAGAGGATTC TGATGAGCCT TTAGAGAGAA GGCTGTCCTT AGTACCAGAT
TCTGAGCAGG GAGAGGCGAT ACTGCCTCGC ATCAGCGTGA TCAGCACTGG CCCCACGCTT
CAGGCACGAA GGAGGCAGTC TGTCCTGAAC CTGATGACAC ACTCAGTTAA CCAAGGTCAG
AACATTCACC GAAAGACAAC AGCATCCACA CGAAAAGTGT CACTGGCCCC TCAGGCAAAC
TTGACTGAAC TGGATATATA TTCAAGAAGG TTATCTCAAG AAACTGGCTT GGAAATAAGT
GAAGAAATTA ACGAAGAAGA CTTAAAGGAG TGCTTTTTTG ATGATATGGA GAGCATACCA
GCAGTGACTA CATGGAACAC ATACCTTCGA TATATTACTG TCCACAAGAG CTTAATTTTT
GTGCTAATTT GGTGCTTAGT AATTTTTCTG GCAGAGGTGG CTGCTTCTTT GGTTGTGCTG
TGGCTCCTTG GAAACACTCC TCTTCAAGAC AAAGGGAATA GTACTCATAG TAGAAATAAC
AGCTATGCAG TGATTATCAC CAGCACCAGT TCGTATTATG TGTTTTACAT TTACGTGGGA
GTAGCCGACA CTTTGCTTGC TATGGGATTC TTCAGAGGTC TACCACTGGT GCATACTCTA
ATCACAGTGT CGAAAATTTT ACACCACAAA ATGTTACATT CTGTTCTTCA AGCACCTATG
TCAACCCTCA ACACGTTGAA AGCAGGTGGG ATTCTTAATA GATTCTCCAA AGATATAGCA
ATTTTGGATG ACCTTCTGCC TCTTACCATA TTTGACTTCA TCCAGTTGTT ATTAATTGTG
ATTGGAGCTA TAGCAGTTGT CGCAGTTTTA CAACCCTACA TCTTTGTTGC AACAGTGCCA
GTGATAGTGG CTTTTATTAT GTTGAGAGCA TATTTCCTCC AAACCTCACA GCAACTCAAA
CAACTGGAAT CTGAAGGCAG GAGTCCAATT TTCACTCATC TTGTTACAAG CTTAAAAGGA
CTATGGACAC TTCGTGCCTT CGGACGGCAG CCTTACTTTG AAACTCTGTT CCACAAAGCT
CTGAATTTAC ATACTGCCAA CTGGTTCTTG TACCTGTCAA CACTGCGCTG GTTCCAAATG
AGAATAGAAA TGATTTTTGT CATCTTCTTC ATTGCTGTTA CCTTCATTTC CATTTTAACA
ACAGGAGAAG GAGAAGGAAG AGTTGGTATT ATCCTGACTT TAGCCATGAA TATCATGAGT
ACATTGCAGT GGGCTGTAAA CTCCAGCATA GATGTGGATA GCTTGATGCG ATCTGTGAGC
CGAGTCTTTA AGTTCATTGA CATGCCAACA GAAGGTAAAC CTACCAAGTC AACCAAACCA
TACAAGAATG GCCAACTCTC GAAAGTTATG ATTATTGAGA ATTCACACGT GAAGAAAGAT
GACATCTGGC CCTCAGGGGG CCAAATGACT GTCAAAGATC TCACAGCAAA ATACACAGAA
GGTGGAAATG CCATATTAGA GAACATTTCC TTCTCAATAA GTCCTGGCCA GAGGGTGGGC
CTCTTGGGAA GAACTGGATC AGGGAAGAGT ACTTTGTTAT CAGCTTTTTT GAGACTACTG
AACACTGAAG GAGAAATCCA GATCGATGGT GTGTCTTGGG ATTCAATAAC TTTGCAACAG
TGGAGGAAAG CCTTTGGAGT GATACCACAG AAAGTATTTA TTTTTTCTGG AACATTTAGA
AAAAACTTGG ATCCCTATGA ACAGTGGAGT GATCAAGAAA TATGGAAAGT TGCAGATGAG
GTTGGGCTCA GATCTGTGAT AGAACAGTTT CCTGGGAAGC TTGACTTTGT CCTTGTGGAT
GGGGGCTGTG TCCTAAGCCA TGGCCACAAG CAGTTGATGT GCTTGGCTAG ATCTGTTCTC
AGTAAGGCGA AGATCTTGCT GCTTGATGAA CCCAGTGCTC ATTTGGATCC AGTAACATAC
CAAATAATTA GAAGAACTCT AAAACAAGCA TTTGCTGATT GCACAGTAAT TCTCTGTGAA
CACAGGATAG AAGCAATGCT GGAATGCCAA CAATTTTTGG TCATAGAAGA GAACAAAGTG
CGGCAGTACG ATTCCATCCA GAAACTGCTG AACGAGAGGA GCCTCTTCCG GCAAGCCATC
AGCCCCTCCG ACAGGGTGAA GCTCTTTCCC CACCGGAACT CAAGCAAGTG CAAGTCTAAG
CCCCAGATTG CTGCTCTGAA AGAGGAGACA GAAGAAGAGG TGCAAGATAC AAGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514055.1
CDS466..4665
Misc Feature(1)466..4662(+)
Misc Feature(2)469..1272(+)
Misc Feature(3)1387..2232(+)
Misc Feature(4)1594..1617(+)
Misc Feature(5)1603..2037(+)
Misc Feature(6)1690..1701(+)
Misc Feature(7)1864..1893(+)
Misc Feature(8)1924..1941(+)
Misc Feature(9)1948..1959(+)
Misc Feature(10)2023..2043(+)
Misc Feature(11)2137..2772(+)
Misc Feature(12)2806..3663(+)
Misc Feature(13)3844..4662(+)
Misc Feature(14)3952..3975(+)
Misc Feature(15)3961..4428(+)
Misc Feature(16)4084..4095(+)
Misc Feature(17)4258..4287(+)
Misc Feature(18)4318..4335(+)
Misc Feature(19)4342..4353(+)
Misc Feature(20)4414..4434(+)
Translation

Target ORF information:

RefSeq Version XM_011515753
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515753

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGTTCTATG GAATCTTTTT ATATTTAGGG GAAGTCACCA AAGCAGTACA GCCTCTCTTA 
CTGGGAAGAA TCATAGCTTC CTATGACCCG GATAACAAGG AGGAACGCTC TATCGCGATT
TATCTAGGCA TAGGCTTATG CCTTCTCTTT ATTGTGAGGA CACTGCTCCT ACACCCAGCC
ATTTTTGGCC TTCATCACAT TGGAATGCAG ATGAGAATAG CTATGTTTAG TTTGATTTAT
AAGAAGACTT TAAAGCTGTC AAGCCGTGTT CTAGATAAAA TAAGTATTGG ACAACTTGTT
AGTCTCCTTT CCAACAACCT GAACAAATTT GATGAAGGAC TTGCATTGGC ACATTTCGTG
TGGATCGCTC CTTTGCAAGT GGCACTCCTC ATGGGGCTAA TCTGGGAGTT GTTACAGGCG
TCTGCCTTCT GTGGACTTGG TTTCCTGATA GTCCTTGCCC TTTTTCAGGC TGGGCTAGGG
AGAATGATGA TGAAGTACAG AGATCAGAGA GCTGGGAAGA TCAGTGAAAG ACTTGTGATT
ACCTCAGAAA TGATTGAAAA TATCCAATCT GTTAAGGCAT ACTGCTGGGA AGAAGCAATG
GAAAAAATGA TTGAAAACTT AAGACAAACA GAACTGAAAC TGACTCGGAA GGCAGCCTAT
GTGAGATACT TCAATAGCTC AGCCTTCTTC TTCTCAGGGT TCTTTGTGGT GTTTTTATCT
GTGCTTCCCT ATGCACTAAT CAAAGGAATC ATCCTCCGGA AAATATTCAC CACCATCTCA
TTCTGCATTG TTCTGCGCAT GGCGGTCACT CGGCAATTTC CCTGGGCTGT ACAAACATGG
TATGACTCTC TTGGAGCAAT AAACAAAATA CAGGATTTCT TACAAAAGCA AGAATATAAG
ACATTGGAAT ATAACTTAAC GACTACAGAA GTAGTGATGG AGAATGTAAC AGCCTTCTGG
GAGGAGGGAT TTGGGGAATT ATTTGAGAAA GCAAAACAAA ACAATAACAA TAGAAAAACT
TCTAATGGTG ATGACAGCCT CTTCTTCAGT AATTTCTCAC TTCTTGGTAC TCCTGTCCTG
AAAGATATTA ATTTCAAGAT AGAAAGAGGA CAGTTGTTGG CGGTTGCTGG ATCCACTGGA
GCAGGCAAGA CTTCACTTCT AATGGTGATT ATGGGAGAAC TGGAGCCTTC AGAGGGTAAA
ATTAAGCACA GTGGAAGAAT TTCATTCTGT TCTCAGTTTT CCTGGATTAT GCCTGGCACC
ATTAAAGAAA ATATCATCTT TGGTGTTTCC TATGATGAAT ATAGATACAG AAGCGTCATC
AAAGCATGCC AACTAGAAGA GGACATCTCC AAGTTTGCAG AGAAAGACAA TATAGTTCTT
GGAGAAGGTG GAATCACACT GAGTGGAGGT CAACGAGCAA GAATTTCTTT AGCAAGAGCA
GTATACAAAG ATGCTGATTT GTATTTATTA GACTCTCCTT TTGGATACCT AGATGTTTTA
ACAGAAAAAG AAATATTTGA AAGCTGTGTC TGTAAACTGA TGGCTAACAA AACTAGGATT
TTGGTCACTT CTAAAATGGA ACATTTAAAG AAAGCTGACA AAATATTAAT TTTGCATGAA
GGTAGCAGCT ATTTTTATGG GACATTTTCA GAACTCCAAA ATCTACAGCC AGACTTTAGC
TCAAAACTCA TGGGATGTGA TTCTTTCGAC CAATTTAGTG CAGAAAGAAG AAATTCAATC
CTAACTGAGA CCTTACACCG TTTCTCATTA GAAGGAGATG CTCCTGTCTC CTGGACAGAA
ACAAAAAAAC AATCTTTTAA ACAGACTGGA GAGTTTGGGG AAAAAAGGAA GAATTCTATT
CTCAATCCAA TCAACTCTAT ACGAAAATTT TCCATTGTGC AAAAGACTCC CTTACAAATG
AATGGCATCG AAGAGGATTC TGATGAGCCT TTAGAGAGAA GGCTGTCCTT AGTACCAGAT
TCTGAGCAGG GAGAGGCGAT ACTGCCTCGC ATCAGCGTGA TCAGCACTGG CCCCACGCTT
CAGGCACGAA GGAGGCAGTC TGTCCTGAAC CTGATGACAC ACTCAGTTAA CCAAGGTCAG
AACATTCACC GAAAGACAAC AGCATCCACA CGAAAAGTGT CACTGGCCCC TCAGGCAAAC
TTGACTGAAC TGGATATATA TTCAAGAAGG TTATCTCAAG AAACTGGCTT GGAAATAAGT
GAAGAAATTA ACGAAGAAGA CTTAAAGGAG TGCTTTTTTG ATGATATGGA GAGCATACCA
GCAGTGACTA CATGGAACAC ATACCTTCGA TATATTACTG TCCACAAGAG CTTAATTTTT
GTGCTAATTT GGTGCTTAGT AATTTTTCTG GCAGAGGTGG CTGCTTCTTT GGTTGTGCTG
TGGCTCCTTG GAAACACTCC TCTTCAAGAC AAAGGGAATA GTACTCATAG TAGAAATAAC
AGCTATGCAG TGATTATCAC CAGCACCAGT TCGTATTATG TGTTTTACAT TTACGTGGGA
GTAGCCGACA CTTTGCTTGC TATGGGATTC TTCAGAGGTC TACCACTGGT GCATACTCTA
ATCACAGTGT CGAAAATTTT ACACCACAAA ATGTTACATT CTGTTCTTCA AGCACCTATG
TCAACCCTCA ACACGTTGAA AGCAGGTGGG ATTCTTAATA GATTCTCCAA AGATATAGCA
ATTTTGGATG ACCTTCTGCC TCTTACCATA TTTGACTTCA TCCAGTTGTT ATTAATTGTG
ATTGGAGCTA TAGCAGTTGT CGCAGTTTTA CAACCCTACA TCTTTGTTGC AACAGTGCCA
GTGATAGTGG CTTTTATTAT GTTGAGAGCA TATTTCCTCC AAACCTCACA GCAACTCAAA
CAACTGGAAT CTGAAGGCAG GAGTCCAATT TTCACTCATC TTGTTACAAG CTTAAAAGGA
CTATGGACAC TTCGTGCCTT CGGACGGCAG CCTTACTTTG AAACTCTGTT CCACAAAGCT
CTGAATTTAC ATACTGCCAA CTGGTTCTTG TACCTGTCAA CACTGCGCTG GTTCCAAATG
AGAATAGAAA TGATTTTTGT CATCTTCTTC ATTGCTGTTA CCTTCATTTC CATTTTAACA
ACAGGAGAAG GAGAAGGAAG AGTTGGTATT ATCCTGACTT TAGCCATGAA TATCATGAGT
ACATTGCAGT GGGCTGTAAA CTCCAGCATA GATGTGGATA GCTTGATGCG ATCTGTGAGC
CGAGTCTTTA AGTTCATTGA CATGCCAACA GAAGGTAAAC CTACCAAGTC AACCAAACCA
TACAAGAATG GCCAACTCTC GAAAGTTATG ATTATTGAGA ATTCACACGT GAAGAAAGAT
GACATCTGGC CCTCAGGGGG CCAAATGACT GTCAAAGATC TCACAGCAAA ATACACAGAA
GGTGGAAATG CCATATTAGA GAACATTTCC TTCTCAATAA GTCCTGGCCA GAGGGTGGGC
CTCTTGGGAA GAACTGGATC AGGGAAGAGT ACTTTGTTAT CAGCTTTTTT GAGACTACTG
AACACTGAAG GAGAAATCCA GATCGATGGT GTGTCTTGGG ATTCAATAAC TTTGCAACAG
TGGAGGAAAG CCTTTGGAGT GATACCACAG AAAGTATTTA TTTTTTCTGG AACATTTAGA
AAAAACTTGG ATCCCTATGA ACAGTGGAGT GATCAAGAAA TATGGAAAGT TGCAGATGAG
GTTGGGCTCA GATCTGTGAT AGAACAGTTT CCTGGGAAGC TTGACTTTGT CCTTGTGGAT
GGGGGCTGTG TCCTAAGCCA TGGCCACAAG CAGTTGATGT GCTTGGCTAG ATCTGTTCTC
AGTAAGGCGA AGATCTTGCT GCTTGATGAA CCCAGTGCTC ATTTGGATCC AGTAACATAC
CAAATAATTA GAAGAACTCT AAAACAAGCA TTTGCTGATT GCACAGTAAT TCTCTGTGAA
CACAGGATAG AAGCAATGCT GGAATGCCAA CAATTTTTGG TCATAGAAGA GAACAAAGTG
CGGCAGTACG ATTCCATCCA GAAACTGCTG AACGAGAGGA GCCTCTTCCG GCAAGCCATC
AGCCCCTCCG ACAGGGTGAA GCTCTTTCCC CACCGGAACT CAAGCAAGTG CAAGTCTAAG
CCCCAGATTG CTGCTCTGAA AGAGGAGACA GAAGAAGAGG TGCAAGATAC AAGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56828
Clone ID Related Accession (Same CDS sequence) XM_011515753 , XM_011515754
Accession Version XM_011515754.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cystic fibrosis transmembrane conductance regulator isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_007933.16) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGTTCTATG GAATCTTTTT ATATTTAGGG GAAGTCACCA AAGCAGTACA GCCTCTCTTA 
CTGGGAAGAA TCATAGCTTC CTATGACCCG GATAACAAGG AGGAACGCTC TATCGCGATT
TATCTAGGCA TAGGCTTATG CCTTCTCTTT ATTGTGAGGA CACTGCTCCT ACACCCAGCC
ATTTTTGGCC TTCATCACAT TGGAATGCAG ATGAGAATAG CTATGTTTAG TTTGATTTAT
AAGAAGACTT TAAAGCTGTC AAGCCGTGTT CTAGATAAAA TAAGTATTGG ACAACTTGTT
AGTCTCCTTT CCAACAACCT GAACAAATTT GATGAAGGAC TTGCATTGGC ACATTTCGTG
TGGATCGCTC CTTTGCAAGT GGCACTCCTC ATGGGGCTAA TCTGGGAGTT GTTACAGGCG
TCTGCCTTCT GTGGACTTGG TTTCCTGATA GTCCTTGCCC TTTTTCAGGC TGGGCTAGGG
AGAATGATGA TGAAGTACAG AGATCAGAGA GCTGGGAAGA TCAGTGAAAG ACTTGTGATT
ACCTCAGAAA TGATTGAAAA TATCCAATCT GTTAAGGCAT ACTGCTGGGA AGAAGCAATG
GAAAAAATGA TTGAAAACTT AAGACAAACA GAACTGAAAC TGACTCGGAA GGCAGCCTAT
GTGAGATACT TCAATAGCTC AGCCTTCTTC TTCTCAGGGT TCTTTGTGGT GTTTTTATCT
GTGCTTCCCT ATGCACTAAT CAAAGGAATC ATCCTCCGGA AAATATTCAC CACCATCTCA
TTCTGCATTG TTCTGCGCAT GGCGGTCACT CGGCAATTTC CCTGGGCTGT ACAAACATGG
TATGACTCTC TTGGAGCAAT AAACAAAATA CAGGATTTCT TACAAAAGCA AGAATATAAG
ACATTGGAAT ATAACTTAAC GACTACAGAA GTAGTGATGG AGAATGTAAC AGCCTTCTGG
GAGGAGGGAT TTGGGGAATT ATTTGAGAAA GCAAAACAAA ACAATAACAA TAGAAAAACT
TCTAATGGTG ATGACAGCCT CTTCTTCAGT AATTTCTCAC TTCTTGGTAC TCCTGTCCTG
AAAGATATTA ATTTCAAGAT AGAAAGAGGA CAGTTGTTGG CGGTTGCTGG ATCCACTGGA
GCAGGCAAGA CTTCACTTCT AATGGTGATT ATGGGAGAAC TGGAGCCTTC AGAGGGTAAA
ATTAAGCACA GTGGAAGAAT TTCATTCTGT TCTCAGTTTT CCTGGATTAT GCCTGGCACC
ATTAAAGAAA ATATCATCTT TGGTGTTTCC TATGATGAAT ATAGATACAG AAGCGTCATC
AAAGCATGCC AACTAGAAGA GGACATCTCC AAGTTTGCAG AGAAAGACAA TATAGTTCTT
GGAGAAGGTG GAATCACACT GAGTGGAGGT CAACGAGCAA GAATTTCTTT AGCAAGAGCA
GTATACAAAG ATGCTGATTT GTATTTATTA GACTCTCCTT TTGGATACCT AGATGTTTTA
ACAGAAAAAG AAATATTTGA AAGCTGTGTC TGTAAACTGA TGGCTAACAA AACTAGGATT
TTGGTCACTT CTAAAATGGA ACATTTAAAG AAAGCTGACA AAATATTAAT TTTGCATGAA
GGTAGCAGCT ATTTTTATGG GACATTTTCA GAACTCCAAA ATCTACAGCC AGACTTTAGC
TCAAAACTCA TGGGATGTGA TTCTTTCGAC CAATTTAGTG CAGAAAGAAG AAATTCAATC
CTAACTGAGA CCTTACACCG TTTCTCATTA GAAGGAGATG CTCCTGTCTC CTGGACAGAA
ACAAAAAAAC AATCTTTTAA ACAGACTGGA GAGTTTGGGG AAAAAAGGAA GAATTCTATT
CTCAATCCAA TCAACTCTAT ACGAAAATTT TCCATTGTGC AAAAGACTCC CTTACAAATG
AATGGCATCG AAGAGGATTC TGATGAGCCT TTAGAGAGAA GGCTGTCCTT AGTACCAGAT
TCTGAGCAGG GAGAGGCGAT ACTGCCTCGC ATCAGCGTGA TCAGCACTGG CCCCACGCTT
CAGGCACGAA GGAGGCAGTC TGTCCTGAAC CTGATGACAC ACTCAGTTAA CCAAGGTCAG
AACATTCACC GAAAGACAAC AGCATCCACA CGAAAAGTGT CACTGGCCCC TCAGGCAAAC
TTGACTGAAC TGGATATATA TTCAAGAAGG TTATCTCAAG AAACTGGCTT GGAAATAAGT
GAAGAAATTA ACGAAGAAGA CTTAAAGGAG TGCTTTTTTG ATGATATGGA GAGCATACCA
GCAGTGACTA CATGGAACAC ATACCTTCGA TATATTACTG TCCACAAGAG CTTAATTTTT
GTGCTAATTT GGTGCTTAGT AATTTTTCTG GCAGAGGTGG CTGCTTCTTT GGTTGTGCTG
TGGCTCCTTG GAAACACTCC TCTTCAAGAC AAAGGGAATA GTACTCATAG TAGAAATAAC
AGCTATGCAG TGATTATCAC CAGCACCAGT TCGTATTATG TGTTTTACAT TTACGTGGGA
GTAGCCGACA CTTTGCTTGC TATGGGATTC TTCAGAGGTC TACCACTGGT GCATACTCTA
ATCACAGTGT CGAAAATTTT ACACCACAAA ATGTTACATT CTGTTCTTCA AGCACCTATG
TCAACCCTCA ACACGTTGAA AGCAGGTGGG ATTCTTAATA GATTCTCCAA AGATATAGCA
ATTTTGGATG ACCTTCTGCC TCTTACCATA TTTGACTTCA TCCAGTTGTT ATTAATTGTG
ATTGGAGCTA TAGCAGTTGT CGCAGTTTTA CAACCCTACA TCTTTGTTGC AACAGTGCCA
GTGATAGTGG CTTTTATTAT GTTGAGAGCA TATTTCCTCC AAACCTCACA GCAACTCAAA
CAACTGGAAT CTGAAGGCAG GAGTCCAATT TTCACTCATC TTGTTACAAG CTTAAAAGGA
CTATGGACAC TTCGTGCCTT CGGACGGCAG CCTTACTTTG AAACTCTGTT CCACAAAGCT
CTGAATTTAC ATACTGCCAA CTGGTTCTTG TACCTGTCAA CACTGCGCTG GTTCCAAATG
AGAATAGAAA TGATTTTTGT CATCTTCTTC ATTGCTGTTA CCTTCATTTC CATTTTAACA
ACAGGAGAAG GAGAAGGAAG AGTTGGTATT ATCCTGACTT TAGCCATGAA TATCATGAGT
ACATTGCAGT GGGCTGTAAA CTCCAGCATA GATGTGGATA GCTTGATGCG ATCTGTGAGC
CGAGTCTTTA AGTTCATTGA CATGCCAACA GAAGGTAAAC CTACCAAGTC AACCAAACCA
TACAAGAATG GCCAACTCTC GAAAGTTATG ATTATTGAGA ATTCACACGT GAAGAAAGAT
GACATCTGGC CCTCAGGGGG CCAAATGACT GTCAAAGATC TCACAGCAAA ATACACAGAA
GGTGGAAATG CCATATTAGA GAACATTTCC TTCTCAATAA GTCCTGGCCA GAGGGTGGGC
CTCTTGGGAA GAACTGGATC AGGGAAGAGT ACTTTGTTAT CAGCTTTTTT GAGACTACTG
AACACTGAAG GAGAAATCCA GATCGATGGT GTGTCTTGGG ATTCAATAAC TTTGCAACAG
TGGAGGAAAG CCTTTGGAGT GATACCACAG AAAGTATTTA TTTTTTCTGG AACATTTAGA
AAAAACTTGG ATCCCTATGA ACAGTGGAGT GATCAAGAAA TATGGAAAGT TGCAGATGAG
GTTGGGCTCA GATCTGTGAT AGAACAGTTT CCTGGGAAGC TTGACTTTGT CCTTGTGGAT
GGGGGCTGTG TCCTAAGCCA TGGCCACAAG CAGTTGATGT GCTTGGCTAG ATCTGTTCTC
AGTAAGGCGA AGATCTTGCT GCTTGATGAA CCCAGTGCTC ATTTGGATCC AGTAACATAC
CAAATAATTA GAAGAACTCT AAAACAAGCA TTTGCTGATT GCACAGTAAT TCTCTGTGAA
CACAGGATAG AAGCAATGCT GGAATGCCAA CAATTTTTGG TCATAGAAGA GAACAAAGTG
CGGCAGTACG ATTCCATCCA GAAACTGCTG AACGAGAGGA GCCTCTTCCG GCAAGCCATC
AGCCCCTCCG ACAGGGTGAA GCTCTTTCCC CACCGGAACT CAAGCAAGTG CAAGTCTAAG
CCCCAGATTG CTGCTCTGAA AGAGGAGACA GAAGAAGAGG TGCAAGATAC AAGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011514056.1
CDS1384..5583
Misc Feature(1)1384..5580(+)
Misc Feature(2)1387..2190(+)
Misc Feature(3)2305..3150(+)
Misc Feature(4)2512..2535(+)
Misc Feature(5)2521..2955(+)
Misc Feature(6)2608..2619(+)
Misc Feature(7)2782..2811(+)
Misc Feature(8)2842..2859(+)
Misc Feature(9)2866..2877(+)
Misc Feature(10)2941..2961(+)
Misc Feature(11)3055..3690(+)
Misc Feature(12)3724..4581(+)
Misc Feature(13)4762..5580(+)
Misc Feature(14)4870..4893(+)
Misc Feature(15)4879..5346(+)
Misc Feature(16)5002..5013(+)
Misc Feature(17)5176..5205(+)
Misc Feature(18)5236..5253(+)
Misc Feature(19)5260..5271(+)
Misc Feature(20)5332..5352(+)
Translation

Target ORF information:

RefSeq Version XM_011515754
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011515754

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
ATGTTCTATG GAATCTTTTT ATATTTAGGG GAAGTCACCA AAGCAGTACA GCCTCTCTTA 
CTGGGAAGAA TCATAGCTTC CTATGACCCG GATAACAAGG AGGAACGCTC TATCGCGATT
TATCTAGGCA TAGGCTTATG CCTTCTCTTT ATTGTGAGGA CACTGCTCCT ACACCCAGCC
ATTTTTGGCC TTCATCACAT TGGAATGCAG ATGAGAATAG CTATGTTTAG TTTGATTTAT
AAGAAGACTT TAAAGCTGTC AAGCCGTGTT CTAGATAAAA TAAGTATTGG ACAACTTGTT
AGTCTCCTTT CCAACAACCT GAACAAATTT GATGAAGGAC TTGCATTGGC ACATTTCGTG
TGGATCGCTC CTTTGCAAGT GGCACTCCTC ATGGGGCTAA TCTGGGAGTT GTTACAGGCG
TCTGCCTTCT GTGGACTTGG TTTCCTGATA GTCCTTGCCC TTTTTCAGGC TGGGCTAGGG
AGAATGATGA TGAAGTACAG AGATCAGAGA GCTGGGAAGA TCAGTGAAAG ACTTGTGATT
ACCTCAGAAA TGATTGAAAA TATCCAATCT GTTAAGGCAT ACTGCTGGGA AGAAGCAATG
GAAAAAATGA TTGAAAACTT AAGACAAACA GAACTGAAAC TGACTCGGAA GGCAGCCTAT
GTGAGATACT TCAATAGCTC AGCCTTCTTC TTCTCAGGGT TCTTTGTGGT GTTTTTATCT
GTGCTTCCCT ATGCACTAAT CAAAGGAATC ATCCTCCGGA AAATATTCAC CACCATCTCA
TTCTGCATTG TTCTGCGCAT GGCGGTCACT CGGCAATTTC CCTGGGCTGT ACAAACATGG
TATGACTCTC TTGGAGCAAT AAACAAAATA CAGGATTTCT TACAAAAGCA AGAATATAAG
ACATTGGAAT ATAACTTAAC GACTACAGAA GTAGTGATGG AGAATGTAAC AGCCTTCTGG
GAGGAGGGAT TTGGGGAATT ATTTGAGAAA GCAAAACAAA ACAATAACAA TAGAAAAACT
TCTAATGGTG ATGACAGCCT CTTCTTCAGT AATTTCTCAC TTCTTGGTAC TCCTGTCCTG
AAAGATATTA ATTTCAAGAT AGAAAGAGGA CAGTTGTTGG CGGTTGCTGG ATCCACTGGA
GCAGGCAAGA CTTCACTTCT AATGGTGATT ATGGGAGAAC TGGAGCCTTC AGAGGGTAAA
ATTAAGCACA GTGGAAGAAT TTCATTCTGT TCTCAGTTTT CCTGGATTAT GCCTGGCACC
ATTAAAGAAA ATATCATCTT TGGTGTTTCC TATGATGAAT ATAGATACAG AAGCGTCATC
AAAGCATGCC AACTAGAAGA GGACATCTCC AAGTTTGCAG AGAAAGACAA TATAGTTCTT
GGAGAAGGTG GAATCACACT GAGTGGAGGT CAACGAGCAA GAATTTCTTT AGCAAGAGCA
GTATACAAAG ATGCTGATTT GTATTTATTA GACTCTCCTT TTGGATACCT AGATGTTTTA
ACAGAAAAAG AAATATTTGA AAGCTGTGTC TGTAAACTGA TGGCTAACAA AACTAGGATT
TTGGTCACTT CTAAAATGGA ACATTTAAAG AAAGCTGACA AAATATTAAT TTTGCATGAA
GGTAGCAGCT ATTTTTATGG GACATTTTCA GAACTCCAAA ATCTACAGCC AGACTTTAGC
TCAAAACTCA TGGGATGTGA TTCTTTCGAC CAATTTAGTG CAGAAAGAAG AAATTCAATC
CTAACTGAGA CCTTACACCG TTTCTCATTA GAAGGAGATG CTCCTGTCTC CTGGACAGAA
ACAAAAAAAC AATCTTTTAA ACAGACTGGA GAGTTTGGGG AAAAAAGGAA GAATTCTATT
CTCAATCCAA TCAACTCTAT ACGAAAATTT TCCATTGTGC AAAAGACTCC CTTACAAATG
AATGGCATCG AAGAGGATTC TGATGAGCCT TTAGAGAGAA GGCTGTCCTT AGTACCAGAT
TCTGAGCAGG GAGAGGCGAT ACTGCCTCGC ATCAGCGTGA TCAGCACTGG CCCCACGCTT
CAGGCACGAA GGAGGCAGTC TGTCCTGAAC CTGATGACAC ACTCAGTTAA CCAAGGTCAG
AACATTCACC GAAAGACAAC AGCATCCACA CGAAAAGTGT CACTGGCCCC TCAGGCAAAC
TTGACTGAAC TGGATATATA TTCAAGAAGG TTATCTCAAG AAACTGGCTT GGAAATAAGT
GAAGAAATTA ACGAAGAAGA CTTAAAGGAG TGCTTTTTTG ATGATATGGA GAGCATACCA
GCAGTGACTA CATGGAACAC ATACCTTCGA TATATTACTG TCCACAAGAG CTTAATTTTT
GTGCTAATTT GGTGCTTAGT AATTTTTCTG GCAGAGGTGG CTGCTTCTTT GGTTGTGCTG
TGGCTCCTTG GAAACACTCC TCTTCAAGAC AAAGGGAATA GTACTCATAG TAGAAATAAC
AGCTATGCAG TGATTATCAC CAGCACCAGT TCGTATTATG TGTTTTACAT TTACGTGGGA
GTAGCCGACA CTTTGCTTGC TATGGGATTC TTCAGAGGTC TACCACTGGT GCATACTCTA
ATCACAGTGT CGAAAATTTT ACACCACAAA ATGTTACATT CTGTTCTTCA AGCACCTATG
TCAACCCTCA ACACGTTGAA AGCAGGTGGG ATTCTTAATA GATTCTCCAA AGATATAGCA
ATTTTGGATG ACCTTCTGCC TCTTACCATA TTTGACTTCA TCCAGTTGTT ATTAATTGTG
ATTGGAGCTA TAGCAGTTGT CGCAGTTTTA CAACCCTACA TCTTTGTTGC AACAGTGCCA
GTGATAGTGG CTTTTATTAT GTTGAGAGCA TATTTCCTCC AAACCTCACA GCAACTCAAA
CAACTGGAAT CTGAAGGCAG GAGTCCAATT TTCACTCATC TTGTTACAAG CTTAAAAGGA
CTATGGACAC TTCGTGCCTT CGGACGGCAG CCTTACTTTG AAACTCTGTT CCACAAAGCT
CTGAATTTAC ATACTGCCAA CTGGTTCTTG TACCTGTCAA CACTGCGCTG GTTCCAAATG
AGAATAGAAA TGATTTTTGT CATCTTCTTC ATTGCTGTTA CCTTCATTTC CATTTTAACA
ACAGGAGAAG GAGAAGGAAG AGTTGGTATT ATCCTGACTT TAGCCATGAA TATCATGAGT
ACATTGCAGT GGGCTGTAAA CTCCAGCATA GATGTGGATA GCTTGATGCG ATCTGTGAGC
CGAGTCTTTA AGTTCATTGA CATGCCAACA GAAGGTAAAC CTACCAAGTC AACCAAACCA
TACAAGAATG GCCAACTCTC GAAAGTTATG ATTATTGAGA ATTCACACGT GAAGAAAGAT
GACATCTGGC CCTCAGGGGG CCAAATGACT GTCAAAGATC TCACAGCAAA ATACACAGAA
GGTGGAAATG CCATATTAGA GAACATTTCC TTCTCAATAA GTCCTGGCCA GAGGGTGGGC
CTCTTGGGAA GAACTGGATC AGGGAAGAGT ACTTTGTTAT CAGCTTTTTT GAGACTACTG
AACACTGAAG GAGAAATCCA GATCGATGGT GTGTCTTGGG ATTCAATAAC TTTGCAACAG
TGGAGGAAAG CCTTTGGAGT GATACCACAG AAAGTATTTA TTTTTTCTGG AACATTTAGA
AAAAACTTGG ATCCCTATGA ACAGTGGAGT GATCAAGAAA TATGGAAAGT TGCAGATGAG
GTTGGGCTCA GATCTGTGAT AGAACAGTTT CCTGGGAAGC TTGACTTTGT CCTTGTGGAT
GGGGGCTGTG TCCTAAGCCA TGGCCACAAG CAGTTGATGT GCTTGGCTAG ATCTGTTCTC
AGTAAGGCGA AGATCTTGCT GCTTGATGAA CCCAGTGCTC ATTTGGATCC AGTAACATAC
CAAATAATTA GAAGAACTCT AAAACAAGCA TTTGCTGATT GCACAGTAAT TCTCTGTGAA
CACAGGATAG AAGCAATGCT GGAATGCCAA CAATTTTTGG TCATAGAAGA GAACAAAGTG
CGGCAGTACG ATTCCATCCA GAAACTGCTG AACGAGAGGA GCCTCTTCCG GCAAGCCATC
AGCCCCTCCG ACAGGGTGAA GCTCTTTCCC CACCGGAACT CAAGCAAGTG CAAGTCTAAG
CCCCAGATTG CTGCTCTGAA AGAGGAGACA GAAGAAGAGG TGCAAGATAC AAGGCTTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27239
Clone ID Related Accession (Same CDS sequence) NM_000492
Accession Version NM_000492.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cystic fibrosis transmembrane conductance regulator
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M28668.1, AC000111.1 and AC000061.1. This sequence is a reference standard in the RefSeqGene project. On Mar 24, 2006 this sequence version replaced gi:6995995. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M28668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGCAGAGGT CGCCTCTGGA AAAGGCCAGC GTTGTCTCCA AACTTTTTTT CAGCTGGACC 
AGACCAATTT TGAGGAAAGG ATACAGACAG CGCCTGGAAT TGTCAGACAT ATACCAAATC
CCTTCTGTTG ATTCTGCTGA CAATCTATCT GAAAAATTGG AAAGAGAATG GGATAGAGAG
CTGGCTTCAA AGAAAAATCC TAAACTCATT AATGCCCTTC GGCGATGTTT TTTCTGGAGA
TTTATGTTCT ATGGAATCTT TTTATATTTA GGGGAAGTCA CCAAAGCAGT ACAGCCTCTC
TTACTGGGAA GAATCATAGC TTCCTATGAC CCGGATAACA AGGAGGAACG CTCTATCGCG
ATTTATCTAG GCATAGGCTT ATGCCTTCTC TTTATTGTGA GGACACTGCT CCTACACCCA
GCCATTTTTG GCCTTCATCA CATTGGAATG CAGATGAGAA TAGCTATGTT TAGTTTGATT
TATAAGAAGA CTTTAAAGCT GTCAAGCCGT GTTCTAGATA AAATAAGTAT TGGACAACTT
GTTAGTCTCC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCATT GGCACATTTC
GTGTGGATCG CTCCTTTGCA AGTGGCACTC CTCATGGGGC TAATCTGGGA GTTGTTACAG
GCGTCTGCCT TCTGTGGACT TGGTTTCCTG ATAGTCCTTG CCCTTTTTCA GGCTGGGCTA
GGGAGAATGA TGATGAAGTA CAGAGATCAG AGAGCTGGGA AGATCAGTGA AAGACTTGTG
ATTACCTCAG AAATGATTGA AAATATCCAA TCTGTTAAGG CATACTGCTG GGAAGAAGCA
ATGGAAAAAA TGATTGAAAA CTTAAGACAA ACAGAACTGA AACTGACTCG GAAGGCAGCC
TATGTGAGAT ACTTCAATAG CTCAGCCTTC TTCTTCTCAG GGTTCTTTGT GGTGTTTTTA
TCTGTGCTTC CCTATGCACT AATCAAAGGA ATCATCCTCC GGAAAATATT CACCACCATC
TCATTCTGCA TTGTTCTGCG CATGGCGGTC ACTCGGCAAT TTCCCTGGGC TGTACAAACA
TGGTATGACT CTCTTGGAGC AATAAACAAA ATACAGGATT TCTTACAAAA GCAAGAATAT
AAGACATTGG AATATAACTT AACGACTACA GAAGTAGTGA TGGAGAATGT AACAGCCTTC
TGGGAGGAGG GATTTGGGGA ATTATTTGAG AAAGCAAAAC AAAACAATAA CAATAGAAAA
ACTTCTAATG GTGATGACAG CCTCTTCTTC AGTAATTTCT CACTTCTTGG TACTCCTGTC
CTGAAAGATA TTAATTTCAA GATAGAAAGA GGACAGTTGT TGGCGGTTGC TGGATCCACT
GGAGCAGGCA AGACTTCACT TCTAATGGTG ATTATGGGAG AACTGGAGCC TTCAGAGGGT
AAAATTAAGC ACAGTGGAAG AATTTCATTC TGTTCTCAGT TTTCCTGGAT TATGCCTGGC
ACCATTAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AATATAGATA CAGAAGCGTC
ATCAAAGCAT GCCAACTAGA AGAGGACATC TCCAAGTTTG CAGAGAAAGA CAATATAGTT
CTTGGAGAAG GTGGAATCAC ACTGAGTGGA GGTCAACGAG CAAGAATTTC TTTAGCAAGA
GCAGTATACA AAGATGCTGA TTTGTATTTA TTAGACTCTC CTTTTGGATA CCTAGATGTT
TTAACAGAAA AAGAAATATT TGAAAGCTGT GTCTGTAAAC TGATGGCTAA CAAAACTAGG
ATTTTGGTCA CTTCTAAAAT GGAACATTTA AAGAAAGCTG ACAAAATATT AATTTTGCAT
GAAGGTAGCA GCTATTTTTA TGGGACATTT TCAGAACTCC AAAATCTACA GCCAGACTTT
AGCTCAAAAC TCATGGGATG TGATTCTTTC GACCAATTTA GTGCAGAAAG AAGAAATTCA
ATCCTAACTG AGACCTTACA CCGTTTCTCA TTAGAAGGAG ATGCTCCTGT CTCCTGGACA
GAAACAAAAA AACAATCTTT TAAACAGACT GGAGAGTTTG GGGAAAAAAG GAAGAATTCT
ATTCTCAATC CAATCAACTC TATACGAAAA TTTTCCATTG TGCAAAAGAC TCCCTTACAA
ATGAATGGCA TCGAAGAGGA TTCTGATGAG CCTTTAGAGA GAAGGCTGTC CTTAGTACCA
GATTCTGAGC AGGGAGAGGC GATACTGCCT CGCATCAGCG TGATCAGCAC TGGCCCCACG
CTTCAGGCAC GAAGGAGGCA GTCTGTCCTG AACCTGATGA CACACTCAGT TAACCAAGGT
CAGAACATTC ACCGAAAGAC AACAGCATCC ACACGAAAAG TGTCACTGGC CCCTCAGGCA
AACTTGACTG AACTGGATAT ATATTCAAGA AGGTTATCTC AAGAAACTGG CTTGGAAATA
AGTGAAGAAA TTAACGAAGA AGACTTAAAG GAGTGCTTTT TTGATGATAT GGAGAGCATA
CCAGCAGTGA CTACATGGAA CACATACCTT CGATATATTA CTGTCCACAA GAGCTTAATT
TTTGTGCTAA TTTGGTGCTT AGTAATTTTT CTGGCAGAGG TGGCTGCTTC TTTGGTTGTG
CTGTGGCTCC TTGGAAACAC TCCTCTTCAA GACAAAGGGA ATAGTACTCA TAGTAGAAAT
AACAGCTATG CAGTGATTAT CACCAGCACC AGTTCGTATT ATGTGTTTTA CATTTACGTG
GGAGTAGCCG ACACTTTGCT TGCTATGGGA TTCTTCAGAG GTCTACCACT GGTGCATACT
CTAATCACAG TGTCGAAAAT TTTACACCAC AAAATGTTAC ATTCTGTTCT TCAAGCACCT
ATGTCAACCC TCAACACGTT GAAAGCAGGT GGGATTCTTA ATAGATTCTC CAAAGATATA
GCAATTTTGG ATGACCTTCT GCCTCTTACC ATATTTGACT TCATCCAGTT GTTATTAATT
GTGATTGGAG CTATAGCAGT TGTCGCAGTT TTACAACCCT ACATCTTTGT TGCAACAGTG
CCAGTGATAG TGGCTTTTAT TATGTTGAGA GCATATTTCC TCCAAACCTC ACAGCAACTC
AAACAACTGG AATCTGAAGG CAGGAGTCCA ATTTTCACTC ATCTTGTTAC AAGCTTAAAA
GGACTATGGA CACTTCGTGC CTTCGGACGG CAGCCTTACT TTGAAACTCT GTTCCACAAA
GCTCTGAATT TACATACTGC CAACTGGTTC TTGTACCTGT CAACACTGCG CTGGTTCCAA
ATGAGAATAG AAATGATTTT TGTCATCTTC TTCATTGCTG TTACCTTCAT TTCCATTTTA
ACAACAGGAG AAGGAGAAGG AAGAGTTGGT ATTATCCTGA CTTTAGCCAT GAATATCATG
AGTACATTGC AGTGGGCTGT AAACTCCAGC ATAGATGTGG ATAGCTTGAT GCGATCTGTG
AGCCGAGTCT TTAAGTTCAT TGACATGCCA ACAGAAGGTA AACCTACCAA GTCAACCAAA
CCATACAAGA ATGGCCAACT CTCGAAAGTT ATGATTATTG AGAATTCACA CGTGAAGAAA
GATGACATCT GGCCCTCAGG GGGCCAAATG ACTGTCAAAG ATCTCACAGC AAAATACACA
GAAGGTGGAA ATGCCATATT AGAGAACATT TCCTTCTCAA TAAGTCCTGG CCAGAGGGTG
GGCCTCTTGG GAAGAACTGG ATCAGGGAAG AGTACTTTGT TATCAGCTTT TTTGAGACTA
CTGAACACTG AAGGAGAAAT CCAGATCGAT GGTGTGTCTT GGGATTCAAT AACTTTGCAA
CAGTGGAGGA AAGCCTTTGG AGTGATACCA CAGAAAGTAT TTATTTTTTC TGGAACATTT
AGAAAAAACT TGGATCCCTA TGAACAGTGG AGTGATCAAG AAATATGGAA AGTTGCAGAT
GAGGTTGGGC TCAGATCTGT GATAGAACAG TTTCCTGGGA AGCTTGACTT TGTCCTTGTG
GATGGGGGCT GTGTCCTAAG CCATGGCCAC AAGCAGTTGA TGTGCTTGGC TAGATCTGTT
CTCAGTAAGG CGAAGATCTT GCTGCTTGAT GAACCCAGTG CTCATTTGGA TCCAGTAACA
TACCAAATAA TTAGAAGAAC TCTAAAACAA GCATTTGCTG ATTGCACAGT AATTCTCTGT
GAACACAGGA TAGAAGCAAT GCTGGAATGC CAACAATTTT TGGTCATAGA AGAGAACAAA
GTGCGGCAGT ACGATTCCAT CCAGAAACTG CTGAACGAGA GGAGCCTCTT CCGGCAAGCC
ATCAGCCCCT CCGACAGGGT GAAGCTCTTT CCCCACCGGA ACTCAAGCAA GTGCAAGTCT
AAGCCCCAGA TTGCTGCTCT GAAAGAGGAG ACAGAAGAAG AGGTGCAAGA TACAAGGCTT
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000483.3
CDS133..4575
Misc Feature(1)67..69(+)
Misc Feature(2)133..4572(+)
Misc Feature(3)373..441(+)
Misc Feature(4)379..1182(+)
Misc Feature(5)484..546(+)
Misc Feature(6)715..777(+)
Misc Feature(7)793..855(+)
Misc Feature(8)1003..1005(+)
Misc Feature(9)1054..1116(+)
Misc Feature(10)1123..1182(+)
Misc Feature(11)1297..2142(+)
Misc Feature(12)1504..1527(+)
Misc Feature(13)1513..1947(+)
Misc Feature(14)1600..1611(+)
Misc Feature(15)1774..1803(+)
Misc Feature(16)1777..1779(+)
Misc Feature(17)1834..1851(+)
Misc Feature(18)1858..1869(+)
Misc Feature(19)1933..1953(+)
Misc Feature(20)2047..2682(+)
Misc Feature(21)2110..2112(+)
Misc Feature(22)2110..2112(+)
Misc Feature(23)2188..2190(+)
Misc Feature(24)2188..2190(+)
Misc Feature(25)2230..2232(+)
Misc Feature(26)2230..2232(+)
Misc Feature(27)2230..2232(+)
Misc Feature(28)2266..2268(+)
Misc Feature(29)2281..2283(+)
Misc Feature(30)2341..2343(+)
Misc Feature(31)2341..2343(+)
Misc Feature(32)2341..2343(+)
Misc Feature(33)2389..2391(+)
Misc Feature(34)2434..2436(+)
Misc Feature(35)2434..2436(+)
Misc Feature(36)2434..2436(+)
Misc Feature(37)2500..2502(+)
Misc Feature(38)2500..2502(+)
Misc Feature(39)2515..2517(+)
Misc Feature(40)2515..2517(+)
Misc Feature(41)2515..2517(+)
Misc Feature(42)2569..2571(+)
Misc Feature(43)2569..2571(+)
Misc Feature(44)2569..2571(+)
Misc Feature(45)2710..2772(+)
Misc Feature(46)2716..3573(+)
Misc Feature(47)2866..2928(+)
Misc Feature(48)3103..3165(+)
Misc Feature(49)3172..3234(+)
Misc Feature(50)3439..3501(+)
Misc Feature(51)3517..3579(+)
Misc Feature(52)3754..4572(+)
Misc Feature(53)3862..3885(+)
Misc Feature(54)3871..4338(+)
Misc Feature(55)3994..4005(+)
Misc Feature(56)4168..4197(+)
Misc Feature(57)4228..4245(+)
Misc Feature(58)4252..4263(+)
Misc Feature(59)4324..4344(+)
Misc Feature(60)4462..4464(+)
Misc Feature(61)4498..4500(+)
Misc Feature(62)4564..4572(+)
Exon (1)1..185
Gene:CFTR
Gene Synonym:
Exon (2)186..296
Gene:CFTR
Gene Synonym:
Exon (3)297..405
Gene:CFTR
Gene Synonym:
Exon (4)406..621
Gene:CFTR
Gene Synonym:
Exon (5)622..711
Gene:CFTR
Gene Synonym:
Exon (6)712..875
Gene:CFTR
Gene Synonym:
Exon (7)876..1001
Gene:CFTR
Gene Synonym:
Exon (8)1002..1248
Gene:CFTR
Gene Synonym:
Exon (9)1249..1341
Gene:CFTR
Gene Synonym:
Exon (10)1342..1524
Gene:CFTR
Gene Synonym:
Exon (11)1525..1716
Gene:CFTR
Gene Synonym:
Exon (12)1717..1811
Gene:CFTR
Gene Synonym:
Exon (13)1812..1898
Gene:CFTR
Gene Synonym:
Exon (14)1899..2622
Gene:CFTR
Gene Synonym:
Exon (15)2623..2751
Gene:CFTR
Gene Synonym:
Exon (16)2752..2789
Gene:CFTR
Gene Synonym:
Exon (17)2790..3040
Gene:CFTR
Gene Synonym:
Exon (18)3041..3120
Gene:CFTR
Gene Synonym:
Exon (19)3121..3271
Gene:CFTR
Gene Synonym:
Exon (20)3272..3499
Gene:CFTR
Gene Synonym:
Exon (21)3500..3600
Gene:CFTR
Gene Synonym:
Exon (22)3601..3849
Gene:CFTR
Gene Synonym:
Exon (23)3850..4005
Gene:CFTR
Gene Synonym:
Exon (24)4006..4095
Gene:CFTR
Gene Synonym:
Exon (25)4096..4268
Gene:CFTR
Gene Synonym:
Exon (26)4269..4374
Gene:CFTR
Gene Synonym:
Exon (27)4375..6132
Gene:CFTR
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000492
Organism Homo sapiens (human)
Definition Homo sapiens cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) (CFTR), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000492

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
ATGCAGAGGT CGCCTCTGGA AAAGGCCAGC GTTGTCTCCA AACTTTTTTT CAGCTGGACC 
AGACCAATTT TGAGGAAAGG ATACAGACAG CGCCTGGAAT TGTCAGACAT ATACCAAATC
CCTTCTGTTG ATTCTGCTGA CAATCTATCT GAAAAATTGG AAAGAGAATG GGATAGAGAG
CTGGCTTCAA AGAAAAATCC TAAACTCATT AATGCCCTTC GGCGATGTTT TTTCTGGAGA
TTTATGTTCT ATGGAATCTT TTTATATTTA GGGGAAGTCA CCAAAGCAGT ACAGCCTCTC
TTACTGGGAA GAATCATAGC TTCCTATGAC CCGGATAACA AGGAGGAACG CTCTATCGCG
ATTTATCTAG GCATAGGCTT ATGCCTTCTC TTTATTGTGA GGACACTGCT CCTACACCCA
GCCATTTTTG GCCTTCATCA CATTGGAATG CAGATGAGAA TAGCTATGTT TAGTTTGATT
TATAAGAAGA CTTTAAAGCT GTCAAGCCGT GTTCTAGATA AAATAAGTAT TGGACAACTT
GTTAGTCTCC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCATT GGCACATTTC
GTGTGGATCG CTCCTTTGCA AGTGGCACTC CTCATGGGGC TAATCTGGGA GTTGTTACAG
GCGTCTGCCT TCTGTGGACT TGGTTTCCTG ATAGTCCTTG CCCTTTTTCA GGCTGGGCTA
GGGAGAATGA TGATGAAGTA CAGAGATCAG AGAGCTGGGA AGATCAGTGA AAGACTTGTG
ATTACCTCAG AAATGATTGA AAATATCCAA TCTGTTAAGG CATACTGCTG GGAAGAAGCA
ATGGAAAAAA TGATTGAAAA CTTAAGACAA ACAGAACTGA AACTGACTCG GAAGGCAGCC
TATGTGAGAT ACTTCAATAG CTCAGCCTTC TTCTTCTCAG GGTTCTTTGT GGTGTTTTTA
TCTGTGCTTC CCTATGCACT AATCAAAGGA ATCATCCTCC GGAAAATATT CACCACCATC
TCATTCTGCA TTGTTCTGCG CATGGCGGTC ACTCGGCAAT TTCCCTGGGC TGTACAAACA
TGGTATGACT CTCTTGGAGC AATAAACAAA ATACAGGATT TCTTACAAAA GCAAGAATAT
AAGACATTGG AATATAACTT AACGACTACA GAAGTAGTGA TGGAGAATGT AACAGCCTTC
TGGGAGGAGG GATTTGGGGA ATTATTTGAG AAAGCAAAAC AAAACAATAA CAATAGAAAA
ACTTCTAATG GTGATGACAG CCTCTTCTTC AGTAATTTCT CACTTCTTGG TACTCCTGTC
CTGAAAGATA TTAATTTCAA GATAGAAAGA GGACAGTTGT TGGCGGTTGC TGGATCCACT
GGAGCAGGCA AGACTTCACT TCTAATGGTG ATTATGGGAG AACTGGAGCC TTCAGAGGGT
AAAATTAAGC ACAGTGGAAG AATTTCATTC TGTTCTCAGT TTTCCTGGAT TATGCCTGGC
ACCATTAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AATATAGATA CAGAAGCGTC
ATCAAAGCAT GCCAACTAGA AGAGGACATC TCCAAGTTTG CAGAGAAAGA CAATATAGTT
CTTGGAGAAG GTGGAATCAC ACTGAGTGGA GGTCAACGAG CAAGAATTTC TTTAGCAAGA
GCAGTATACA AAGATGCTGA TTTGTATTTA TTAGACTCTC CTTTTGGATA CCTAGATGTT
TTAACAGAAA AAGAAATATT TGAAAGCTGT GTCTGTAAAC TGATGGCTAA CAAAACTAGG
ATTTTGGTCA CTTCTAAAAT GGAACATTTA AAGAAAGCTG ACAAAATATT AATTTTGCAT
GAAGGTAGCA GCTATTTTTA TGGGACATTT TCAGAACTCC AAAATCTACA GCCAGACTTT
AGCTCAAAAC TCATGGGATG TGATTCTTTC GACCAATTTA GTGCAGAAAG AAGAAATTCA
ATCCTAACTG AGACCTTACA CCGTTTCTCA TTAGAAGGAG ATGCTCCTGT CTCCTGGACA
GAAACAAAAA AACAATCTTT TAAACAGACT GGAGAGTTTG GGGAAAAAAG GAAGAATTCT
ATTCTCAATC CAATCAACTC TATACGAAAA TTTTCCATTG TGCAAAAGAC TCCCTTACAA
ATGAATGGCA TCGAAGAGGA TTCTGATGAG CCTTTAGAGA GAAGGCTGTC CTTAGTACCA
GATTCTGAGC AGGGAGAGGC GATACTGCCT CGCATCAGCG TGATCAGCAC TGGCCCCACG
CTTCAGGCAC GAAGGAGGCA GTCTGTCCTG AACCTGATGA CACACTCAGT TAACCAAGGT
CAGAACATTC ACCGAAAGAC AACAGCATCC ACACGAAAAG TGTCACTGGC CCCTCAGGCA
AACTTGACTG AACTGGATAT ATATTCAAGA AGGTTATCTC AAGAAACTGG CTTGGAAATA
AGTGAAGAAA TTAACGAAGA AGACTTAAAG GAGTGCTTTT TTGATGATAT GGAGAGCATA
CCAGCAGTGA CTACATGGAA CACATACCTT CGATATATTA CTGTCCACAA GAGCTTAATT
TTTGTGCTAA TTTGGTGCTT AGTAATTTTT CTGGCAGAGG TGGCTGCTTC TTTGGTTGTG
CTGTGGCTCC TTGGAAACAC TCCTCTTCAA GACAAAGGGA ATAGTACTCA TAGTAGAAAT
AACAGCTATG CAGTGATTAT CACCAGCACC AGTTCGTATT ATGTGTTTTA CATTTACGTG
GGAGTAGCCG ACACTTTGCT TGCTATGGGA TTCTTCAGAG GTCTACCACT GGTGCATACT
CTAATCACAG TGTCGAAAAT TTTACACCAC AAAATGTTAC ATTCTGTTCT TCAAGCACCT
ATGTCAACCC TCAACACGTT GAAAGCAGGT GGGATTCTTA ATAGATTCTC CAAAGATATA
GCAATTTTGG ATGACCTTCT GCCTCTTACC ATATTTGACT TCATCCAGTT GTTATTAATT
GTGATTGGAG CTATAGCAGT TGTCGCAGTT TTACAACCCT ACATCTTTGT TGCAACAGTG
CCAGTGATAG TGGCTTTTAT TATGTTGAGA GCATATTTCC TCCAAACCTC ACAGCAACTC
AAACAACTGG AATCTGAAGG CAGGAGTCCA ATTTTCACTC ATCTTGTTAC AAGCTTAAAA
GGACTATGGA CACTTCGTGC CTTCGGACGG CAGCCTTACT TTGAAACTCT GTTCCACAAA
GCTCTGAATT TACATACTGC CAACTGGTTC TTGTACCTGT CAACACTGCG CTGGTTCCAA
ATGAGAATAG AAATGATTTT TGTCATCTTC TTCATTGCTG TTACCTTCAT TTCCATTTTA
ACAACAGGAG AAGGAGAAGG AAGAGTTGGT ATTATCCTGA CTTTAGCCAT GAATATCATG
AGTACATTGC AGTGGGCTGT AAACTCCAGC ATAGATGTGG ATAGCTTGAT GCGATCTGTG
AGCCGAGTCT TTAAGTTCAT TGACATGCCA ACAGAAGGTA AACCTACCAA GTCAACCAAA
CCATACAAGA ATGGCCAACT CTCGAAAGTT ATGATTATTG AGAATTCACA CGTGAAGAAA
GATGACATCT GGCCCTCAGG GGGCCAAATG ACTGTCAAAG ATCTCACAGC AAAATACACA
GAAGGTGGAA ATGCCATATT AGAGAACATT TCCTTCTCAA TAAGTCCTGG CCAGAGGGTG
GGCCTCTTGG GAAGAACTGG ATCAGGGAAG AGTACTTTGT TATCAGCTTT TTTGAGACTA
CTGAACACTG AAGGAGAAAT CCAGATCGAT GGTGTGTCTT GGGATTCAAT AACTTTGCAA
CAGTGGAGGA AAGCCTTTGG AGTGATACCA CAGAAAGTAT TTATTTTTTC TGGAACATTT
AGAAAAAACT TGGATCCCTA TGAACAGTGG AGTGATCAAG AAATATGGAA AGTTGCAGAT
GAGGTTGGGC TCAGATCTGT GATAGAACAG TTTCCTGGGA AGCTTGACTT TGTCCTTGTG
GATGGGGGCT GTGTCCTAAG CCATGGCCAC AAGCAGTTGA TGTGCTTGGC TAGATCTGTT
CTCAGTAAGG CGAAGATCTT GCTGCTTGAT GAACCCAGTG CTCATTTGGA TCCAGTAACA
TACCAAATAA TTAGAAGAAC TCTAAAACAA GCATTTGCTG ATTGCACAGT AATTCTCTGT
GAACACAGGA TAGAAGCAAT GCTGGAATGC CAACAATTTT TGGTCATAGA AGAGAACAAA
GTGCGGCAGT ACGATTCCAT CCAGAAACTG CTGAACGAGA GGAGCCTCTT CCGGCAAGCC
ATCAGCCCCT CCGACAGGGT GAAGCTCTTT CCCCACCGGA ACTCAAGCAA GTGCAAGTCT
AAGCCCCAGA TTGCTGCTCT GAAAGAGGAG ACAGAAGAAG AGGTGCAAGA TACAAGGCTT
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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