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NEU3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NEU3
Entrez Gene ID 10825
Full Name sialidase 3 (membrane sialidase)
Synonyms SIAL3
General protein information
Preferred Names
sialidase-3
Names
sialidase-3
neuraminidase 3
membrane sialidase
ganglioside sialidase
N-acetyl-alpha-neuraminidase 3
NP_006647.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q13.5

Summary This gene product belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. It is localized in the plasma membrane, and its activity is specific for gangliosides. It may play a role in modulating the ganglioside content of the lipid bilayer. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

604617


mRNA and Protein(s)

mRNA Protein Name
NM_006656 NP_006647 sialidase-3



Homo sapiens (human) NEU3 NP_006647.3
Pan troglodytes (chimpanzee) NEU3 XP_003952031.1
Macaca mulatta (Rhesus monkey) LOC694310 XP_002808128.1
Canis lupus familiaris (dog) NEU3 XP_005633558.1
Bos taurus (cattle) NEU3 NP_776547.2
Mus musculus (house mouse) Neu3 NP_057929.1
Rattus norvegicus (Norway rat) Neu3 NP_446462.1
Gallus gallus (chicken) NEU3 XP_428099.4
Danio rerio (zebrafish) neu3.2 NP_001003644.2
Danio rerio (zebrafish) neu3.3 NP_001071006.1
Danio rerio (zebrafish) neu3.4 NP_001103193.1
Danio rerio (zebrafish) neu3.5 NP_001103203.1
Danio rerio (zebrafish) neu3.1 NP_001073133.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100496715 XP_002942460.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NEU3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEU3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu30315
NM_006656 Homo sapiens sialidase 3 (membrane sialidase) (NEU3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30315
Clone ID Related Accession (Same CDS sequence) NM_006656
Accession Version NM_006656.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1386bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product sialidase-3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB240271.1, CN429425.1 and AP001992.4. On Nov 1, 2006 this sequence version replaced gi:21704286. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB240271.1, Y18563.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGACCTG CGGACCTGCC CCCGCGCCCC ATGGAAGAAT CCCCGGCGTC CAGCTCTGCC 
CCGACAGAGA CGGAGGAGCC GGGGTCCAGT GCAGAGGTCA TGGAAGAAGT GACAACATGC
TCCTTCAACA GCCCTCTGTT CCGGCAGGAA GATGACAGAG GGATTACCTA CCGGATCCCA
GCCCTGCTCT ACATACCCCC CACCCACACC TTCCTGGCCT TTGCAGAGAA GCGTTCTACG
AGGAGAGATG AGGATGCTCT CCACCTGGTG CTGAGGCGAG GGTTGAGGAT TGGGCAGTTG
GTACAGTGGG GGCCCCTGAA GCCACTGATG GAAGCCACAC TACCGGGGCA TCGGACCATG
AACCCCTGTC CTGTATGGGA GCAGAAGAGT GGTTGTGTGT TCCTGTTCTT CATCTGTGTG
CGGGGCCATG TCACAGAGCG TCAACAGATT GTGTCAGGCA GGAATGCTGC CCGCCTTTGC
TTCATCTACA GTCAGGATGC TGGATGTTCA TGGAGTGAGG TGAGGGACTT GACTGAGGAG
GTCATTGGCT CAGAGCTGAA GCACTGGGCC ACATTTGCTG TGGGCCCAGG TCATGGCATC
CAGCTGCAGT CAGGGAGACT GGTCATCCCT GCGTATACCT ACTACATCCC TTCCTGGTTC
TTTTGCTTCC AGCTACCATG TAAAACCAGG CCTCATTCTC TGATGATCTA CAGTGATGAC
CTAGGGGTCA CATGGCACCA TGGTAGACTC ATTAGGCCCA TGGTTACAGT AGAATGTGAA
GTGGCAGAGG TGACTGGGAG GGCTGGCCAC CCTGTGCTAT ATTGCAGTGC CCGGACACCA
AACAGGTGCC GGGCAGAGGC GCTCAGCACT GACCATGGTG AAGGCTTTCA GAGACTGGCC
CTGAGTCGAC AGCTCTGTGA GCCCCCACAT GGTTGCCAAG GGAGTGTGGT AAGTTTCCGG
CCCCTGGAGA TCCCACATAG GTGCCAGGAC TCTAGCAGCA AAGATGCACC CACCATTCAG
CAGAGCTCTC CAGGCAGTTC ACTGAGGCTG GAGGAGGAAG CTGGAACACC GTCAGAATCA
TGGCTCTTGT ACTCACACCC AACCAGTAGG AAACAGAGGG TTGACCTAGG TATCTATCTC
AACCAGACCC CCTTGGAGGC TGCCTGCTGG TCCCGCCCCT GGATCTTGCA CTGTGGGCCC
TGTGGCTACT CTGATCTGGC TGCTCTGGAG GAGGAGGGCT TGTTTGGGTG TTTGTTTGAA
TGTGGGACCA AGCAAGAGTG TGAGCAGATT GCCTTCCGCC TGTTTACACA CCGGGAGATC
CTGAGTCACC TGCAGGGGGA CTGCACCAGC CCTGGTAGGA ACCCAAGCCA ATTCAAAAGC
AATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006647.3
CDS157..1542
Misc Feature(1)286..1464(+)
Misc Feature(2)325..486(+)
Misc Feature(3)328..1416(+)
Misc Feature(4)364..1407(+)
Misc Feature(5)511..687(+)
Misc Feature(6)727..906(+)
Misc Feature(7)925..1053(+)
Misc Feature(8)1087..1344(+)
Misc Feature(9)1360..1464(+)
Exon (1)1..250
Gene:NEU3
Gene Synonym:
Exon (2)251..462
Gene:NEU3
Gene Synonym:
Exon (3)463..2748
Gene:NEU3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006656
Organism Homo sapiens (human)
Definition Homo sapiens sialidase 3 (membrane sialidase) (NEU3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006656

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGAGACCTG CGGACCTGCC CCCGCGCCCC ATGGAAGAAT CCCCGGCGTC CAGCTCTGCC 
CCGACAGAGA CGGAGGAGCC GGGGTCCAGT GCAGAGGTCA TGGAAGAAGT GACAACATGC
TCCTTCAACA GCCCTCTGTT CCGGCAGGAA GATGACAGAG GGATTACCTA CCGGATCCCA
GCCCTGCTCT ACATACCCCC CACCCACACC TTCCTGGCCT TTGCAGAGAA GCGTTCTACG
AGGAGAGATG AGGATGCTCT CCACCTGGTG CTGAGGCGAG GGTTGAGGAT TGGGCAGTTG
GTACAGTGGG GGCCCCTGAA GCCACTGATG GAAGCCACAC TACCGGGGCA TCGGACCATG
AACCCCTGTC CTGTATGGGA GCAGAAGAGT GGTTGTGTGT TCCTGTTCTT CATCTGTGTG
CGGGGCCATG TCACAGAGCG TCAACAGATT GTGTCAGGCA GGAATGCTGC CCGCCTTTGC
TTCATCTACA GTCAGGATGC TGGATGTTCA TGGAGTGAGG TGAGGGACTT GACTGAGGAG
GTCATTGGCT CAGAGCTGAA GCACTGGGCC ACATTTGCTG TGGGCCCAGG TCATGGCATC
CAGCTGCAGT CAGGGAGACT GGTCATCCCT GCGTATACCT ACTACATCCC TTCCTGGTTC
TTTTGCTTCC AGCTACCATG TAAAACCAGG CCTCATTCTC TGATGATCTA CAGTGATGAC
CTAGGGGTCA CATGGCACCA TGGTAGACTC ATTAGGCCCA TGGTTACAGT AGAATGTGAA
GTGGCAGAGG TGACTGGGAG GGCTGGCCAC CCTGTGCTAT ATTGCAGTGC CCGGACACCA
AACAGGTGCC GGGCAGAGGC GCTCAGCACT GACCATGGTG AAGGCTTTCA GAGACTGGCC
CTGAGTCGAC AGCTCTGTGA GCCCCCACAT GGTTGCCAAG GGAGTGTGGT AAGTTTCCGG
CCCCTGGAGA TCCCACATAG GTGCCAGGAC TCTAGCAGCA AAGATGCACC CACCATTCAG
CAGAGCTCTC CAGGCAGTTC ACTGAGGCTG GAGGAGGAAG CTGGAACACC GTCAGAATCA
TGGCTCTTGT ACTCACACCC AACCAGTAGG AAACAGAGGG TTGACCTAGG TATCTATCTC
AACCAGACCC CCTTGGAGGC TGCCTGCTGG TCCCGCCCCT GGATCTTGCA CTGTGGGCCC
TGTGGCTACT CTGATCTGGC TGCTCTGGAG GAGGAGGGCT TGTTTGGGTG TTTGTTTGAA
TGTGGGACCA AGCAAGAGTG TGAGCAGATT GCCTTCCGCC TGTTTACACA CCGGGAGATC
CTGAGTCACC TGCAGGGGGA CTGCACCAGC CCTGGTAGGA ACCCAAGCCA ATTCAAAAGC
AATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of lysosomal sialidase NEU1 and plasma membrane sialidase NEU3 in human erythrocytes
J. Cell. Biochem. 114 (1), 204-211 (2013)
D'Avila F, Tringali C, Papini N, Anastasia L, Croci G, Massaccesi L, Monti E, Tettamanti G and Venerando B.


book

The plasma membrane sialidase NEU3 regulates the malignancy of renal carcinoma cells by controlling beta1 integrin internalization and recycling
J. Biol. Chem. 287 (51), 42835-42845 (2012)
Tringali C, Lupo B, Silvestri I, Papini N, Anastasia L, Tettamanti G and Venerando B.


book

Sialidases on mammalian sperm mediate deciduous sialylation during capacitation
J. Biol. Chem. 287 (45), 38073-38079 (2012)
Ma F, Wu D, Deng L, Secrest P, Zhao J, Varki N, Lindheim S and Gagneux P.


book

NEU1 and NEU3 sialidase activity expressed in human lung microvascular endothelia: NEU1 restrains endothelial cell migration, whereas NEU3 does not
J. Biol. Chem. 287 (19), 15966-15980 (2012)
Cross AS, Hyun SW, Miranda-Ribera A, Feng C, Liu A, Nguyen C, Zhang L, Luzina IG, Atamas SP, Twaddell WS, Guang W, Lillehoj EP, Puche AC, Huang W, Wang LX, Passaniti A and Goldblum SE.


book

Membrane sialidase NEU3 is highly expressed in human melanoma cells promoting cell growth with minimal changes in the composition of gangliosides
Cancer Sci. 102 (12), 2139-2149 (2011)
Miyata M, Kambe M, Tajima O, Moriya S, Sawaki H, Hotta H, Kondo Y, Narimatsu H, Miyagi T, Furukawa K and Furukawa K.


book

Substrate specificity and inhibitor studies of a membrane-bound ganglioside sialidase isolated from human brain tissue
Biol. Chem. 383 (11), 1735-1742 (2002)
Oehler C, Kopitz J and Cantz M.


book

Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression
Proc. Natl. Acad. Sci. U.S.A. 99 (16), 10718-10723 (2002)
Kakugawa Y, Wada T, Yamaguchi K, Yamanami H, Ouchi K, Sato I and Miyagi T.


book

A close association of the ganglioside-specific sialidase Neu3 with caveolin in membrane microdomains
J. Biol. Chem. 277 (29), 26252-26259 (2002)
Wang Y, Yamaguchi K, Wada T, Hata K, Zhao X, Fujimoto T and Miyagi T.


book

Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane
Biochem. J. 349 (PT 1), 343-351 (2000)
Monti E, Bassi MT, Papini N, Riboni M, Manzoni M, Venerando B, Croci G, Preti A, Ballabio A, Tettamanti G and Borsani G.


book

Cloning, expression, and chromosomal mapping of a human ganglioside sialidase
Biochem. Biophys. Res. Commun. 261 (1), 21-27 (1999)
Wada T, Yoshikawa Y, Tokuyama S, Kuwabara M, Akita H and Miyagi T.


 
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