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CYP46A1 cytochrome P450, family 46, subfamily A, polypeptide 1 [Homo sapiens (human)]

Gene Symbol CYP46A1
Entrez Gene ID 10858
Full Name cytochrome P450, family 46, subfamily A, polypeptide 1
Synonyms CP46, CYP46
General protein information
Preferred Names
cholesterol 24-hydroxylase
Names
cholesterol 24-hydroxylase
CH24H
cytochrome P450 46A1
cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
NP_006659.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

14

14q32.1

Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

604087

Disorder Html:





The following CYP46A1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP46A1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu64417 XM_011536364 PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu47482 XM_005267274 PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu64418 XM_011536365 PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu18783 NM_006668 Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $419

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu64417
Accession Version XM_011536364.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1473bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cholesterol 24-hydroxylase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ACCGCGCTCG CAGCCGCTAC GAGCACATCC CCGGGCCGCC GCGGCCCAGT
TTCCTTCTAG GACACCTCCC CTGCTTTTGG AAAAAGGATG AGGTTGGTGG CCGTGTGCTC
CAAGATGTGT TTTTGGATTG GGCTAAGAAG TATGGACCTG TTGTGCGGGT CAACGTCTTC
CACAAAACCT CAGTCATCGT CACGAGTCCT GAGTCGGTTA AGAAGTTCCT GATGTCAACC
AAGTACAACA AGGACTCCAA GATGTACCGT GCGCTCCAGA CTGTGTTTGG TGAGAGACTC
TTCGGCCAAG GCTTGGTGTC CGAATGCAAC TATGAGCGCT GGCACAAGCA GCGGAGAGTC
ATAGACCTGG CCTTCAGCCG GAGCTCCTTG GTTAGCTTAA TGGAAACATT CAACGAGAAG
GCTGAGCAGC TGGTGGAGAT TCTAGAAGCC AAGGCAGATG GGCAGACCCC AGTGTCCATG
CAGGACATGC TGACCTACAC CGCCATGGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG
ACCAGTATGC TGCTGGGTGC CCAGAAGCCT CTGTCCCAGG CAGTGAAACT TATGTTGGAG
GGAATCACTG CGTCCCGCAA CACTCTGGCA AAGTTCCTGC CAGGGAAGAG GAAGCAGCTC
CGGGAGGTCC GGGAGAGCAT TCGCTTCCTG CGCCAGGTGG GCAGGGACTG GGTCCAGCGC
CGCCGGGAAG CCCTGAAGAG GGGCGAGGAG GTTCCTGCCG ACATCCTCAC ACAGATTCTG
AAAGCTGAAG AGGGAGCCCA GGACGACGAG GGTCTGCTGG ACAACTTCGT CACCTTCTTC
ATTGCTGGTC ACGAGACCTC TGCCAACCAC TTGGCGTTCA CAGTGATGGA GCTGTCTCGC
CAGCCAGAGA TCGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTCATTGG TTCTAAGAGG
TACCTGGATT TCGAGGACCT GGGGAGACTG CAGTACCTGT CCCAGGTCCT CAAAGAGTCG
CTGAGGCTGT ACCCACCAGC ATGGGGCACC TTTCGCCTGC TGGAAGAGGA GACCTTGATT
GATGGGTTCA GCACCTATGT CATGGGGCGG ATGGACACAT ACTTTGAGGA CCCGCTGACT
TTCAACCCCG ATCGCTTCGG CCCTGGAGCA CCCAAGCCAC GGTTCACCTA CTTCCCCTTC
TCCCTGGGCC ACCGCTCCTG CATCGGGCAG CAGTTTGCTC AGATGGAGGT GAAGGTGGTC
ATGGCAAAGC TGCTGCAGAG GCTGGAGTTC CGGCTGGTGC CCGGGCAGCG CTTCGGGCTG
CAGGAGCAGG CCACACTCAA GCCACTGGAC CCCGTGCTGT GCACCCTGCG GCCCCGCGGC
TGGCAGCCCG CACCCCCACC ACCCCCCTGC TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011534666.1
CDS157..1629
Misc Feature(1)256..1524(+)
Translation
Position Chain Variation Link
113 113 g, t dbSNP:761065912
115 115 c, t dbSNP:766731643
119 119 c, t dbSNP:539709482
122 122 a, c dbSNP:754074927
124 124 c, t dbSNP:755127021
125 125 a, g dbSNP:765218061
133 133 a, c dbSNP:751580896
141 141 g, t dbSNP:757144374
142 142 c, t dbSNP:781047407
150 150 c, t dbSNP:550031516
204 204 a, c, t dbSNP:569975911
231 231 c, t dbSNP:779718133
261 261 a, g dbSNP:748912611
266 266 a, c dbSNP:768274775
278 278 a, t dbSNP:759885529
282 282 g, t dbSNP:769929751
309 309 a, g dbSNP:533477005
328 328 c, g dbSNP:762981146
329 329 a, c, g dbSNP:200569884
346 346 c, t dbSNP:760604778
366 366 g, t dbSNP:774423149
373 373 c, t dbSNP:761826761
375 375 c, t dbSNP:141032633
378 378 c, t dbSNP:556161085
383 383 a, g dbSNP:759291829
390 390 c, t dbSNP:765059068
391 391 a, g dbSNP:752350612
397 397 c, g dbSNP:760979832
399 399 c, g dbSNP:758126503
408 408 a, g dbSNP:763781486
414 414 a, c, t dbSNP:751135557
415 415 a, g dbSNP:781586799
422 422 g, t dbSNP:746361117
432 432 a, g dbSNP:756488741
433 433 a, g dbSNP:780458125
449 449 c, t dbSNP:755299289
462 462 a, c dbSNP:779147763
468 468 a, g dbSNP:748350226
483 483 a, c, t dbSNP:772101757
489 489 a, g dbSNP:747002018
498 498 -, tg dbSNP:749546833
498 498 c, t dbSNP:769792545
519 519 c, t dbSNP:780187016
534 534 g, t dbSNP:144966483
537 537 c, t dbSNP:139245529
538 538 a, g dbSNP:774289118
552 552 c, g dbSNP:761460288
579 579 a, g dbSNP:771783206
580 580 a, g dbSNP:772703431
595 595 c, t dbSNP:760275943
599 599 a, g dbSNP:765829489
605 605 c, t dbSNP:751871147
634 634 a, g dbSNP:75832974
639 639 a, t dbSNP:757555782
651 651 a, g dbSNP:767787355
660 660 a, g dbSNP:750556808
669 669 a, g dbSNP:756225751
679 679 a, c dbSNP:779038416
687 687 a, c dbSNP:748017615
692 692 a, c dbSNP:368496205
698 698 a, t dbSNP:777426743
706 706 c, t dbSNP:746753597
710 710 c, t dbSNP:770584624
714 714 c, t dbSNP:776224827
715 715 a, c, g dbSNP:74730047
718 718 a, g dbSNP:148568536
721 721 a, g dbSNP:776122077
741 741 a, g, t dbSNP:368295416
742 742 a, g dbSNP:745505656
744 744 c, t dbSNP:769357422
757 757 a, t dbSNP:779430731
769 769 c, t dbSNP:748771138
777 777 c, t dbSNP:769062361
780 780 g, t dbSNP:774884916
783 783 a, g dbSNP:762310846
785 785 c, t dbSNP:267604122
789 789 c, g dbSNP:372548812
791 791 c, t dbSNP:773630837
795 795 a, g dbSNP:760975591
809 809 c, t dbSNP:766557064
827 827 c, t dbSNP:753879253
828 828 a, g dbSNP:569614577
837 837 c, t dbSNP:763976882
839 839 c, g dbSNP:751475413
860 860 c, g dbSNP:771302947
869 869 a, t dbSNP:776775506
873 873 a, g dbSNP:746098686
878 878 a, g dbSNP:142905408
887 887 a, g dbSNP:775557276
899 899 a, g dbSNP:762967586
900 900 c, t dbSNP:767470135
907 907 c, t dbSNP:773100496
909 909 c, t dbSNP:760438576
911 911 a, g dbSNP:766185526
924 924 c, t dbSNP:753505149
930 930 c, g dbSNP:754668158
934 934 c, t dbSNP:764924761
935 935 a, c, g dbSNP:752278427
939 939 c, t dbSNP:778466149
941 941 a, g dbSNP:151175005
942 942 c, g dbSNP:757628267
945 945 a, g dbSNP:781623174
954 954 a, g dbSNP:746157910
960 960 c, t dbSNP:770021958
963 963 a, g dbSNP:780207418
974 974 c, t dbSNP:749407644
976 976 a, g dbSNP:768703295
979 979 a, g dbSNP:773194715
984 984 c, t dbSNP:201604068
996 996 a, g, t dbSNP:201085949
1013 1013 c, t dbSNP:775348328
1014 1014 c, t dbSNP:565548234
1017 1017 a, g, t dbSNP:532807703
1020 1020 c, t dbSNP:751098169
1021 1021 a, g dbSNP:761184361
1023 1023 c, t dbSNP:768106991
1028 1028 g, t dbSNP:750870929
1029 1029 a, t dbSNP:543441457
1044 1044 c, t dbSNP:780395313
1045 1045 a, g dbSNP:754138869
1049 1049 c, t dbSNP:755209726
1052 1052 c, t dbSNP:78767954
1068 1068 c, g, t dbSNP:200661407
1086 1086 c, t dbSNP:759997989
1091 1091 c, t dbSNP:765509152
1096 1096 a, g dbSNP:753035877
1115 1115 a, g dbSNP:758560402
1116 1116 c, t dbSNP:370474237
1125 1125 g, t dbSNP:112687989
1128 1128 c, t dbSNP:187491029
1129 1129 a, g dbSNP:757381529
1131 1131 a, g dbSNP:201760580
1132 1132 a, g dbSNP:780189156
1134 1134 a, g dbSNP:372949820
1146 1146 c, t dbSNP:188075791
1166 1166 a, g dbSNP:767699278
1173 1173 a, g dbSNP:750463063
1179 1179 c, t dbSNP:374987652
1188 1188 c, t dbSNP:754886094
1195 1195 c, t dbSNP:778862126
1204 1204 c, t dbSNP:368904176
1209 1209 a, g dbSNP:370665505
1215 1215 g, t dbSNP:758186359
1224 1224 c, g dbSNP:780483949
1230 1230 a, g dbSNP:749795671
1235 1235 c, t dbSNP:769217801
1237 1237 c, g dbSNP:774871671
1257 1257 a, g, t dbSNP:200110496
1265 1265 a, c dbSNP:773452402
1270 1270 c, t dbSNP:760720152
1271 1271 a, g dbSNP:766472276
1272 1272 c, g dbSNP:775360092
1275 1275 a, g dbSNP:762883900
1285 1285 a, g dbSNP:763967249
1287 1287 a, g, t dbSNP:751383710
1290 1290 c, t dbSNP:143992631
1299 1299 c, t dbSNP:750076915
1308 1308 c, t dbSNP:773079915
1311 1311 a, c dbSNP:760348654
1317 1317 c, t dbSNP:766097065
1330 1330 c, g dbSNP:568187423
1336 1336 c, t dbSNP:749206215
1345 1345 a, g dbSNP:764838844
1347 1347 c, t dbSNP:375146687
1350 1350 a, g dbSNP:369668371
1355 1355 a, c dbSNP:778095376
1362 1362 c, t dbSNP:751991710
1365 1365 c, t dbSNP:757537926
1366 1366 a, g dbSNP:557127125
1374 1374 c, t dbSNP:199588723
1375 1375 a, c, g dbSNP:201296209
1378 1378 a, c dbSNP:570769035
1380 1380 c, t dbSNP:749276702
1384 1384 a, g dbSNP:772079641
1385 1385 a, c dbSNP:539341562
1394 1394 c, t dbSNP:776584862
1396 1396 c, t dbSNP:200564104
1401 1401 c, t dbSNP:769604864
1407 1407 c, t dbSNP:200043004
1410 1410 a, c dbSNP:762579312
1413 1413 c, g dbSNP:763503185
1431 1431 -, tcctgca dbSNP:757614243
1433 1433 c, t dbSNP:773842361
1440 1440 c, t dbSNP:201784451
1441 1441 a, g dbSNP:767963054
1446 1446 a, g dbSNP:750815135
1459 1459 a, g dbSNP:144915500
1468 1468 a, c dbSNP:761087218
1476 1476 c, t dbSNP:766726017
1480 1480 a, g dbSNP:754165973
1502 1502 a, t dbSNP:138751864
1503 1503 a, g dbSNP:765455026
1507 1507 c, t dbSNP:148881618
1512 1512 a, g dbSNP:758525166
1514 1514 c, t dbSNP:781333856
1515 1515 a, g dbSNP:750379134
1518 1518 c, t dbSNP:143537324
1525 1525 c, t dbSNP:779723628
1530 1530 c, t dbSNP:749061350
1542 1542 a, g dbSNP:371696839
1554 1554 c, g dbSNP:778594593
1560 1560 a, g dbSNP:747704117
1567 1567 c, t dbSNP:771557532
1569 1569 c, t dbSNP:773777664
1570 1570 g, t dbSNP:200877443
1572 1572 a, g dbSNP:369621046
1581 1581 a, c dbSNP:771415207
1585 1585 c, t dbSNP:369066335
1586 1586 a, g dbSNP:759910002
1589 1589 c, t dbSNP:765421364
1593 1593 c, t dbSNP:550169210
1595 1595 a, g dbSNP:763189041
1599 1599 g, t dbSNP:764272389
1605 1605 c, t dbSNP:751628935
1606 1606 a, g dbSNP:756141971
1609 1609 a, c dbSNP:779999297
1619 1619 c, g dbSNP:753616111
1623 1623 c, t dbSNP:754778906
1631 1631 a, g dbSNP:574424212
1634 1634 a, g dbSNP:778489476
1639 1639 c, t dbSNP:374391699
1648 1648 c, t dbSNP:771647447
1658 1658 c, t dbSNP:777280375
1659 1659 a, g dbSNP:746476117
1670 1670 a, g dbSNP:771375798
1675 1675 c, t dbSNP:777169519
1676 1676 a, g dbSNP:144296616
1694 1694 c, t dbSNP:377757226
1719 1719 c, t dbSNP:187836713
1720 1720 c, t dbSNP:193231294
1721 1721 a, g dbSNP:772737565
1727 1727 c, t dbSNP:185303723
1734 1734 a, c dbSNP:778103184
1741 1741 c, t dbSNP:569611765
1754 1754 c, t dbSNP:529027937
1766 1766 c, t dbSNP:563133856
1770 1770 a, c dbSNP:190228579
1771 1771 g, t dbSNP:548961533
1792 1792 a, g dbSNP:746721057
1803 1803 c, t dbSNP:566088652
1815 1815 a, g dbSNP:148734093
1903 1903 c, t dbSNP:551738077
1921 1921 a, g dbSNP:571800369
1986 1986 c, t dbSNP:537530186
1994 1994 a, g dbSNP:557595465
1995 1995 c, t dbSNP:373000472
2003 2003 c, t dbSNP:142328283
2011 2011 c, t dbSNP:761185977
2015 2015 a, g dbSNP:567810998
2037 2037 a, g dbSNP:536972585
2043 2043 c, t dbSNP:565708040
2058 2058 c, t dbSNP:192811535
2102 2102 a, g dbSNP:540697823
2103 2103 c, t dbSNP:147831141
2104 2104 a, g dbSNP:552070370
2115 2115 -, g dbSNP:767813546
2123 2123 c, t dbSNP:577358612
2124 2124 a, g dbSNP:15506
2175 2175 c, t dbSNP:755269561
2176 2176 a, g dbSNP:543073118
2186 2186 a, g dbSNP:572524340
2190 2190 a, g dbSNP:141330096
2223 2223 c, t dbSNP:767571800

Target ORF information:

RefSeq Version XM_011536364
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536364

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ACCGCGCTCG CAGCCGCTAC GAGCACATCC CCGGGCCGCC GCGGCCCAGT
TTCCTTCTAG GACACCTCCC CTGCTTTTGG AAAAAGGATG AGGTTGGTGG CCGTGTGCTC
CAAGATGTGT TTTTGGATTG GGCTAAGAAG TATGGACCTG TTGTGCGGGT CAACGTCTTC
CACAAAACCT CAGTCATCGT CACGAGTCCT GAGTCGGTTA AGAAGTTCCT GATGTCAACC
AAGTACAACA AGGACTCCAA GATGTACCGT GCGCTCCAGA CTGTGTTTGG TGAGAGACTC
TTCGGCCAAG GCTTGGTGTC CGAATGCAAC TATGAGCGCT GGCACAAGCA GCGGAGAGTC
ATAGACCTGG CCTTCAGCCG GAGCTCCTTG GTTAGCTTAA TGGAAACATT CAACGAGAAG
GCTGAGCAGC TGGTGGAGAT TCTAGAAGCC AAGGCAGATG GGCAGACCCC AGTGTCCATG
CAGGACATGC TGACCTACAC CGCCATGGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG
ACCAGTATGC TGCTGGGTGC CCAGAAGCCT CTGTCCCAGG CAGTGAAACT TATGTTGGAG
GGAATCACTG CGTCCCGCAA CACTCTGGCA AAGTTCCTGC CAGGGAAGAG GAAGCAGCTC
CGGGAGGTCC GGGAGAGCAT TCGCTTCCTG CGCCAGGTGG GCAGGGACTG GGTCCAGCGC
CGCCGGGAAG CCCTGAAGAG GGGCGAGGAG GTTCCTGCCG ACATCCTCAC ACAGATTCTG
AAAGCTGAAG AGGGAGCCCA GGACGACGAG GGTCTGCTGG ACAACTTCGT CACCTTCTTC
ATTGCTGGTC ACGAGACCTC TGCCAACCAC TTGGCGTTCA CAGTGATGGA GCTGTCTCGC
CAGCCAGAGA TCGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTCATTGG TTCTAAGAGG
TACCTGGATT TCGAGGACCT GGGGAGACTG CAGTACCTGT CCCAGGTCCT CAAAGAGTCG
CTGAGGCTGT ACCCACCAGC ATGGGGCACC TTTCGCCTGC TGGAAGAGGA GACCTTGATT
GATGGGTTCA GCACCTATGT CATGGGGCGG ATGGACACAT ACTTTGAGGA CCCGCTGACT
TTCAACCCCG ATCGCTTCGG CCCTGGAGCA CCCAAGCCAC GGTTCACCTA CTTCCCCTTC
TCCCTGGGCC ACCGCTCCTG CATCGGGCAG CAGTTTGCTC AGATGGAGGT GAAGGTGGTC
ATGGCAAAGC TGCTGCAGAG GCTGGAGTTC CGGCTGGTGC CCGGGCAGCG CTTCGGGCTG
CAGGAGCAGG CCACACTCAA GCCACTGGAC CCCGTGCTGT GCACCCTGCG GCCCCGCGGC
TGGCAGCCCG CACCCCCACC ACCCCCCTGC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47482
Accession Version XM_005267274.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1068bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cholesterol 24-hydroxylase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578825418. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAATGCCT CCTTGGTTAG CTTAATGGAA ACATTCAACG AGAAGGCTGA GCAGCTGGTG 
GAGATTCTAG AAGCCAAGGC AGATGGGCAG ACCCCAGTGT CCATGCAGGA CATGCTGACC
TACACCGCCA TGGACATCCT GGCCAAGGCA GCTTTTGGGA TGGAGACCAG TATGCTGCTG
GGTGCCCAGA AGCCTCTGTC CCAGGCAGTG AAACTTATGT TGGAGGGAAT CACTGCGTCC
CGCAACACTC TGGCAAAGTT CCTGCCAGGG AAGAGGAAGC AGCTCCGGGA GGTCCGGGAG
AGCATTCGCT TCCTGCGCCA GGTGGGCAGG GACTGGGTCC AGCGCCGCCG GGAAGCCCTG
AAGAGGGGCG AGGAGGTTCC TGCCGACATC CTCACACAGA TTCTGAAAGC TGAAGAGGGA
GCCCAGGACG ACGAGGGTCT GCTGGACAAC TTCGTCACCT TCTTCATTGC TGGTCACGAG
ACCTCTGCCA ACCACTTGGC GTTCACAGTG ATGGAGCTGT CTCGCCAGCC AGAGATCGTG
GCAAGGCTGC AGGCCGAGGT GGATGAGGTC ATTGGTTCTA AGAGGTACCT GGATTTCGAG
GACCTGGGGA GACTGCAGTA CCTGTCCCAG GTCCTCAAAG AGTCGCTGAG GCTGTACCCA
CCAGCATGGG GCACCTTTCG CCTGCTGGAA GAGGAGACCT TGATTGATGG GGTCAGAGTC
CCCGGCAACA CCCCGCTCTT GTTCAGCACC TATGTCATGG GGCGGATGGA CACATACTTT
GAGGACCCGC TGACTTTCAA CCCCGATCGC TTCGGCCCTG GAGCACCCAA GCCACGGTTC
ACCTACTTCC CCTTCTCCCT GGGCCACCGC TCCTGCATCG GGCAGCAGTT TGCTCAGATG
GAGGTGAAGG TGGTCATGGC AAAGCTGCTG CAGAGGCTGG AGTTCCGGCT GGTGCCCGGG
CAGCGCTTCG GGCTGCAGGA GCAGGCCACA CTCAAGCCAC TGGACCCCGT GCTGTGCACC
CTGCGGCCCC GCGGCTGGCA GCCCGCACCC CCACCACCCC CCTGCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005267331.1
CDS72..1139
Misc Feature(1)90..1034(+)
Translation
Position Chain Variation Link
8 8 a, g dbSNP:532244423
16 16 c, g dbSNP:183780779
85 85 c, t dbSNP:751871147
114 114 a, g dbSNP:75832974
119 119 a, t dbSNP:757555782
131 131 a, g dbSNP:767787355
140 140 a, g dbSNP:750556808
149 149 a, g dbSNP:756225751
159 159 a, c dbSNP:779038416
167 167 a, c dbSNP:748017615
172 172 a, c dbSNP:368496205
178 178 a, t dbSNP:777426743
186 186 c, t dbSNP:746753597
190 190 c, t dbSNP:770584624
194 194 c, t dbSNP:776224827
195 195 a, c, g dbSNP:74730047
198 198 a, g dbSNP:148568536
201 201 a, g dbSNP:776122077
221 221 a, g, t dbSNP:368295416
222 222 a, g dbSNP:745505656
224 224 c, t dbSNP:769357422
237 237 a, t dbSNP:779430731
249 249 c, t dbSNP:748771138
257 257 c, t dbSNP:769062361
260 260 g, t dbSNP:774884916
263 263 a, g dbSNP:762310846
265 265 c, t dbSNP:267604122
269 269 c, g dbSNP:372548812
271 271 c, t dbSNP:773630837
275 275 a, g dbSNP:760975591
289 289 c, t dbSNP:766557064
307 307 c, t dbSNP:753879253
308 308 a, g dbSNP:569614577
317 317 c, t dbSNP:763976882
319 319 c, g dbSNP:751475413
340 340 c, g dbSNP:771302947
349 349 a, t dbSNP:776775506
353 353 a, g dbSNP:746098686
358 358 a, g dbSNP:142905408
367 367 a, g dbSNP:775557276
379 379 a, g dbSNP:762967586
380 380 c, t dbSNP:767470135
387 387 c, t dbSNP:773100496
389 389 c, t dbSNP:760438576
391 391 a, g dbSNP:766185526
404 404 c, t dbSNP:753505149
410 410 c, g dbSNP:754668158
414 414 c, t dbSNP:764924761
415 415 a, c, g dbSNP:752278427
419 419 c, t dbSNP:778466149
421 421 a, g dbSNP:151175005
422 422 c, g dbSNP:757628267
425 425 a, g dbSNP:781623174
434 434 a, g dbSNP:746157910
440 440 c, t dbSNP:770021958
443 443 a, g dbSNP:780207418
454 454 c, t dbSNP:749407644
456 456 a, g dbSNP:768703295
459 459 a, g dbSNP:773194715
464 464 c, t dbSNP:201604068
476 476 a, g, t dbSNP:201085949
493 493 c, t dbSNP:775348328
494 494 c, t dbSNP:565548234
497 497 a, g, t dbSNP:532807703
500 500 c, t dbSNP:751098169
501 501 a, g dbSNP:761184361
503 503 c, t dbSNP:768106991
508 508 g, t dbSNP:750870929
509 509 a, t dbSNP:543441457
524 524 c, t dbSNP:780395313
525 525 a, g dbSNP:754138869
529 529 c, t dbSNP:755209726
532 532 c, t dbSNP:78767954
548 548 c, g, t dbSNP:200661407
566 566 c, t dbSNP:759997989
571 571 c, t dbSNP:765509152
576 576 a, g dbSNP:753035877
595 595 a, g dbSNP:758560402
596 596 c, t dbSNP:370474237
605 605 g, t dbSNP:112687989
608 608 c, t dbSNP:187491029
609 609 a, g dbSNP:757381529
611 611 a, g dbSNP:201760580
612 612 a, g dbSNP:780189156
614 614 a, g dbSNP:372949820
626 626 c, t dbSNP:188075791
646 646 a, g dbSNP:767699278
653 653 a, g dbSNP:750463063
659 659 c, t dbSNP:374987652
668 668 c, t dbSNP:754886094
675 675 c, t dbSNP:778862126
684 684 c, t dbSNP:368904176
689 689 a, g dbSNP:370665505
695 695 g, t dbSNP:758186359
704 704 c, g dbSNP:780483949
710 710 a, g dbSNP:749795671
715 715 c, t dbSNP:769217801
717 717 c, g dbSNP:774871671
737 737 a, g, t dbSNP:200110496
745 745 a, c dbSNP:773452402
750 750 c, t dbSNP:760720152
751 751 a, g dbSNP:766472276
752 752 c, g dbSNP:775360092
755 755 a, g dbSNP:762883900
765 765 a, g dbSNP:763967249
767 767 a, g, t dbSNP:751383710
770 770 c, t dbSNP:143992631
779 779 c, t dbSNP:750076915
791 791 c, g dbSNP:755645477
794 794 c, t dbSNP:779618668
795 795 a, g dbSNP:146435738
800 800 c, t dbSNP:755567035
804 804 c, t dbSNP:779523894
805 805 c, t dbSNP:748564064
806 806 a, g dbSNP:140794624
809 809 c, g dbSNP:550352631
818 818 c, t dbSNP:773079915
821 821 a, c dbSNP:760348654
827 827 c, t dbSNP:766097065
840 840 c, g dbSNP:568187423
846 846 c, t dbSNP:749206215
855 855 a, g dbSNP:764838844
857 857 c, t dbSNP:375146687
860 860 a, g dbSNP:369668371
865 865 a, c dbSNP:778095376
872 872 c, t dbSNP:751991710
875 875 c, t dbSNP:757537926
876 876 a, g dbSNP:557127125
884 884 c, t dbSNP:199588723
885 885 a, c, g dbSNP:201296209
888 888 a, c dbSNP:570769035
890 890 c, t dbSNP:749276702
894 894 a, g dbSNP:772079641
895 895 a, c dbSNP:539341562
904 904 c, t dbSNP:776584862
906 906 c, t dbSNP:200564104
911 911 c, t dbSNP:769604864
917 917 c, t dbSNP:200043004
920 920 a, c dbSNP:762579312
923 923 c, g dbSNP:763503185
941 941 -, tcctgca dbSNP:757614243
943 943 c, t dbSNP:773842361
950 950 c, t dbSNP:201784451
951 951 a, g dbSNP:767963054
956 956 a, g dbSNP:750815135
969 969 a, g dbSNP:144915500
978 978 a, c dbSNP:761087218
986 986 c, t dbSNP:766726017
990 990 a, g dbSNP:754165973
1012 1012 a, t dbSNP:138751864
1013 1013 a, g dbSNP:765455026
1017 1017 c, t dbSNP:148881618
1022 1022 a, g dbSNP:758525166
1024 1024 c, t dbSNP:781333856
1025 1025 a, g dbSNP:750379134
1028 1028 c, t dbSNP:143537324
1035 1035 c, t dbSNP:779723628
1040 1040 c, t dbSNP:749061350
1052 1052 a, g dbSNP:371696839
1064 1064 c, g dbSNP:778594593
1070 1070 a, g dbSNP:747704117
1077 1077 c, t dbSNP:771557532
1079 1079 c, t dbSNP:773777664
1080 1080 g, t dbSNP:200877443
1082 1082 a, g dbSNP:369621046
1091 1091 a, c dbSNP:771415207
1095 1095 c, t dbSNP:369066335
1096 1096 a, g dbSNP:759910002
1099 1099 c, t dbSNP:765421364
1103 1103 c, t dbSNP:550169210
1105 1105 a, g dbSNP:763189041
1109 1109 g, t dbSNP:764272389
1115 1115 c, t dbSNP:751628935
1116 1116 a, g dbSNP:756141971
1119 1119 a, c dbSNP:779999297
1129 1129 c, g dbSNP:753616111
1133 1133 c, t dbSNP:754778906
1141 1141 a, g dbSNP:574424212
1144 1144 a, g dbSNP:778489476
1149 1149 c, t dbSNP:374391699
1158 1158 c, t dbSNP:771647447
1168 1168 c, t dbSNP:777280375
1169 1169 a, g dbSNP:746476117
1180 1180 a, g dbSNP:771375798
1185 1185 c, t dbSNP:777169519
1186 1186 a, g dbSNP:144296616
1204 1204 c, t dbSNP:377757226
1229 1229 c, t dbSNP:187836713
1230 1230 c, t dbSNP:193231294
1231 1231 a, g dbSNP:772737565
1237 1237 c, t dbSNP:185303723
1244 1244 a, c dbSNP:778103184
1251 1251 c, t dbSNP:569611765
1264 1264 c, t dbSNP:529027937
1276 1276 c, t dbSNP:563133856
1280 1280 a, c dbSNP:190228579
1281 1281 g, t dbSNP:548961533
1302 1302 a, g dbSNP:746721057
1313 1313 c, t dbSNP:566088652
1325 1325 a, g dbSNP:148734093
1413 1413 c, t dbSNP:551738077
1431 1431 a, g dbSNP:571800369
1496 1496 c, t dbSNP:537530186
1504 1504 a, g dbSNP:557595465
1505 1505 c, t dbSNP:373000472
1513 1513 c, t dbSNP:142328283
1521 1521 c, t dbSNP:761185977
1525 1525 a, g dbSNP:567810998
1547 1547 a, g dbSNP:536972585
1553 1553 c, t dbSNP:565708040
1568 1568 c, t dbSNP:192811535
1612 1612 a, g dbSNP:540697823
1613 1613 c, t dbSNP:147831141
1614 1614 a, g dbSNP:552070370
1625 1625 -, g dbSNP:767813546
1633 1633 c, t dbSNP:577358612
1634 1634 a, g dbSNP:15506
1685 1685 c, t dbSNP:755269561
1686 1686 a, g dbSNP:543073118
1696 1696 a, g dbSNP:572524340
1700 1700 a, g dbSNP:141330096
1733 1733 c, t dbSNP:767571800

Target ORF information:

RefSeq Version XM_005267274
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005267274

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAATGCCT CCTTGGTTAG CTTAATGGAA ACATTCAACG AGAAGGCTGA GCAGCTGGTG 
GAGATTCTAG AAGCCAAGGC AGATGGGCAG ACCCCAGTGT CCATGCAGGA CATGCTGACC
TACACCGCCA TGGACATCCT GGCCAAGGCA GCTTTTGGGA TGGAGACCAG TATGCTGCTG
GGTGCCCAGA AGCCTCTGTC CCAGGCAGTG AAACTTATGT TGGAGGGAAT CACTGCGTCC
CGCAACACTC TGGCAAAGTT CCTGCCAGGG AAGAGGAAGC AGCTCCGGGA GGTCCGGGAG
AGCATTCGCT TCCTGCGCCA GGTGGGCAGG GACTGGGTCC AGCGCCGCCG GGAAGCCCTG
AAGAGGGGCG AGGAGGTTCC TGCCGACATC CTCACACAGA TTCTGAAAGC TGAAGAGGGA
GCCCAGGACG ACGAGGGTCT GCTGGACAAC TTCGTCACCT TCTTCATTGC TGGTCACGAG
ACCTCTGCCA ACCACTTGGC GTTCACAGTG ATGGAGCTGT CTCGCCAGCC AGAGATCGTG
GCAAGGCTGC AGGCCGAGGT GGATGAGGTC ATTGGTTCTA AGAGGTACCT GGATTTCGAG
GACCTGGGGA GACTGCAGTA CCTGTCCCAG GTCCTCAAAG AGTCGCTGAG GCTGTACCCA
CCAGCATGGG GCACCTTTCG CCTGCTGGAA GAGGAGACCT TGATTGATGG GGTCAGAGTC
CCCGGCAACA CCCCGCTCTT GTTCAGCACC TATGTCATGG GGCGGATGGA CACATACTTT
GAGGACCCGC TGACTTTCAA CCCCGATCGC TTCGGCCCTG GAGCACCCAA GCCACGGTTC
ACCTACTTCC CCTTCTCCCT GGGCCACCGC TCCTGCATCG GGCAGCAGTT TGCTCAGATG
GAGGTGAAGG TGGTCATGGC AAAGCTGCTG CAGAGGCTGG AGTTCCGGCT GGTGCCCGGG
CAGCGCTTCG GGCTGCAGGA GCAGGCCACA CTCAAGCCAC TGGACCCCGT GCTGTGCACC
CTGCGGCCCC GCGGCTGGCA GCCCGCACCC CCACCACCCC CCTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu64418
Accession Version XM_011536365.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1044bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product cholesterol 24-hydroxylase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_026437.13) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAAACAT TCAACGAGAA GGCTGAGCAG CTGGTGGAGA TTCTAGAAGC CAAGGCAGAT 
GGGCAGACCC CAGTGTCCAT GCAGGACATG CTGACCTACA CCGCCATGGA CATCCTGGCC
AAGGCAGCTT TTGGGATGGA GACCAGTATG CTGCTGGGTG CCCAGAAGCC TCTGTCCCAG
GCAGTGAAAC TTATGTTGGA GGGAATCACT GCGTCCCGCA ACACTCTGGC AAAGTTCCTG
CCAGGGAAGA GGAAGCAGCT CCGGGAGGTC CGGGAGAGCA TTCGCTTCCT GCGCCAGGTG
GGCAGGGACT GGGTCCAGCG CCGCCGGGAA GCCCTGAAGA GGGGCGAGGA GGTTCCTGCC
GACATCCTCA CACAGATTCT GAAAGCTGAA GAGGGAGCCC AGGACGACGA GGGTCTGCTG
GACAACTTCG TCACCTTCTT CATTGCTGGT CACGAGACCT CTGCCAACCA CTTGGCGTTC
ACAGTGATGG AGCTGTCTCG CCAGCCAGAG ATCGTGGCAA GGCTGCAGGC CGAGGTGGAT
GAGGTCATTG GTTCTAAGAG GTACCTGGAT TTCGAGGACC TGGGGAGACT GCAGTACCTG
TCCCAGGTCC TCAAAGAGTC GCTGAGGCTG TACCCACCAG CATGGGGCAC CTTTCGCCTG
CTGGAAGAGG AGACCTTGAT TGATGGGGTC AGAGTCCCCG GCAACACCCC GCTCTTGTTC
AGCACCTATG TCATGGGGCG GATGGACACA TACTTTGAGG ACCCGCTGAC TTTCAACCCC
GATCGCTTCG GCCCTGGAGC ACCCAAGCCA CGGTTCACCT ACTTCCCCTT CTCCCTGGGC
CACCGCTCCT GCATCGGGCA GCAGTTTGCT CAGATGGAGG TGAAGGTGGT CATGGCAAAG
CTGCTGCAGA GGCTGGAGTT CCGGCTGGTG CCCGGGCAGC GCTTCGGGCT GCAGGAGCAG
GCCACACTCA AGCCACTGGA CCCCGTGCTG TGCACCCTGC GGCCCCGCGG CTGGCAGCCC
GCACCCCCAC CACCCCCCTG CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011534667.1
CDS741..1784
Misc Feature(1)744..1679(+)
Translation
Position Chain Variation Link
37 37 c, t dbSNP:182103150
109 109 c, t dbSNP:151307540
119 119 c, t dbSNP:573245745
120 120 a, g dbSNP:768956817
123 123 a, t dbSNP:545146617
124 124 a, g dbSNP:34891372
137 137 c, t dbSNP:533507697
140 140 a, g dbSNP:553847629
148 148 a, t dbSNP:111574998
167 167 a, g dbSNP:545891191
173 173 a, g dbSNP:773969121
177 177 c, t dbSNP:556442668
212 212 a, g dbSNP:575273335
218 218 -, a dbSNP:762926179
253 253 a, g dbSNP:542115085
256 256 c, t dbSNP:561785145
289 289 a, g dbSNP:369098068
317 317 a, g dbSNP:150494497
335 335 g, t dbSNP:544217122
377 377 c, t dbSNP:541035865
390 390 c, t dbSNP:35965011
401 401 -, aaat dbSNP:569956334
445 445 c, g dbSNP:115820844
474 474 c, t dbSNP:549861825
490 490 a, g dbSNP:767566547
496 496 c, t dbSNP:12588744
530 530 a, g dbSNP:189152996
545 545 a, g dbSNP:542419104
559 559 c, t dbSNP:566208696
570 570 c, g dbSNP:535310842
605 605 c, t dbSNP:138517205
614 614 a, g dbSNP:2181229
632 632 c, t dbSNP:559540836
654 654 c, t dbSNP:757559068
711 711 c, t dbSNP:539581516
730 730 c, t dbSNP:751871147
759 759 a, g dbSNP:75832974
764 764 a, t dbSNP:757555782
776 776 a, g dbSNP:767787355
785 785 a, g dbSNP:750556808
794 794 a, g dbSNP:756225751
804 804 a, c dbSNP:779038416
812 812 a, c dbSNP:748017615
817 817 a, c dbSNP:368496205
823 823 a, t dbSNP:777426743
831 831 c, t dbSNP:746753597
835 835 c, t dbSNP:770584624
839 839 c, t dbSNP:776224827
840 840 a, c, g dbSNP:74730047
843 843 a, g dbSNP:148568536
846 846 a, g dbSNP:776122077
866 866 a, g, t dbSNP:368295416
867 867 a, g dbSNP:745505656
869 869 c, t dbSNP:769357422
882 882 a, t dbSNP:779430731
894 894 c, t dbSNP:748771138
902 902 c, t dbSNP:769062361
905 905 g, t dbSNP:774884916
908 908 a, g dbSNP:762310846
910 910 c, t dbSNP:267604122
914 914 c, g dbSNP:372548812
916 916 c, t dbSNP:773630837
920 920 a, g dbSNP:760975591
934 934 c, t dbSNP:766557064
952 952 c, t dbSNP:753879253
953 953 a, g dbSNP:569614577
962 962 c, t dbSNP:763976882
964 964 c, g dbSNP:751475413
985 985 c, g dbSNP:771302947
994 994 a, t dbSNP:776775506
998 998 a, g dbSNP:746098686
1003 1003 a, g dbSNP:142905408
1012 1012 a, g dbSNP:775557276
1024 1024 a, g dbSNP:762967586
1025 1025 c, t dbSNP:767470135
1032 1032 c, t dbSNP:773100496
1034 1034 c, t dbSNP:760438576
1036 1036 a, g dbSNP:766185526
1049 1049 c, t dbSNP:753505149
1055 1055 c, g dbSNP:754668158
1059 1059 c, t dbSNP:764924761
1060 1060 a, c, g dbSNP:752278427
1064 1064 c, t dbSNP:778466149
1066 1066 a, g dbSNP:151175005
1067 1067 c, g dbSNP:757628267
1070 1070 a, g dbSNP:781623174
1079 1079 a, g dbSNP:746157910
1085 1085 c, t dbSNP:770021958
1088 1088 a, g dbSNP:780207418
1099 1099 c, t dbSNP:749407644
1101 1101 a, g dbSNP:768703295
1104 1104 a, g dbSNP:773194715
1109 1109 c, t dbSNP:201604068
1121 1121 a, g, t dbSNP:201085949
1138 1138 c, t dbSNP:775348328
1139 1139 c, t dbSNP:565548234
1142 1142 a, g, t dbSNP:532807703
1145 1145 c, t dbSNP:751098169
1146 1146 a, g dbSNP:761184361
1148 1148 c, t dbSNP:768106991
1153 1153 g, t dbSNP:750870929
1154 1154 a, t dbSNP:543441457
1169 1169 c, t dbSNP:780395313
1170 1170 a, g dbSNP:754138869
1174 1174 c, t dbSNP:755209726
1177 1177 c, t dbSNP:78767954
1193 1193 c, g, t dbSNP:200661407
1211 1211 c, t dbSNP:759997989
1216 1216 c, t dbSNP:765509152
1221 1221 a, g dbSNP:753035877
1240 1240 a, g dbSNP:758560402
1241 1241 c, t dbSNP:370474237
1250 1250 g, t dbSNP:112687989
1253 1253 c, t dbSNP:187491029
1254 1254 a, g dbSNP:757381529
1256 1256 a, g dbSNP:201760580
1257 1257 a, g dbSNP:780189156
1259 1259 a, g dbSNP:372949820
1271 1271 c, t dbSNP:188075791
1291 1291 a, g dbSNP:767699278
1298 1298 a, g dbSNP:750463063
1304 1304 c, t dbSNP:374987652
1313 1313 c, t dbSNP:754886094
1320 1320 c, t dbSNP:778862126
1329 1329 c, t dbSNP:368904176
1334 1334 a, g dbSNP:370665505
1340 1340 g, t dbSNP:758186359
1349 1349 c, g dbSNP:780483949
1355 1355 a, g dbSNP:749795671
1360 1360 c, t dbSNP:769217801
1362 1362 c, g dbSNP:774871671
1382 1382 a, g, t dbSNP:200110496
1390 1390 a, c dbSNP:773452402
1395 1395 c, t dbSNP:760720152
1396 1396 a, g dbSNP:766472276
1397 1397 c, g dbSNP:775360092
1400 1400 a, g dbSNP:762883900
1410 1410 a, g dbSNP:763967249
1412 1412 a, g, t dbSNP:751383710
1415 1415 c, t dbSNP:143992631
1424 1424 c, t dbSNP:750076915
1436 1436 c, g dbSNP:755645477
1439 1439 c, t dbSNP:779618668
1440 1440 a, g dbSNP:146435738
1445 1445 c, t dbSNP:755567035
1449 1449 c, t dbSNP:779523894
1450 1450 c, t dbSNP:748564064
1451 1451 a, g dbSNP:140794624
1454 1454 c, g dbSNP:550352631
1463 1463 c, t dbSNP:773079915
1466 1466 a, c dbSNP:760348654
1472 1472 c, t dbSNP:766097065
1485 1485 c, g dbSNP:568187423
1491 1491 c, t dbSNP:749206215
1500 1500 a, g dbSNP:764838844
1502 1502 c, t dbSNP:375146687
1505 1505 a, g dbSNP:369668371
1510 1510 a, c dbSNP:778095376
1517 1517 c, t dbSNP:751991710
1520 1520 c, t dbSNP:757537926
1521 1521 a, g dbSNP:557127125
1529 1529 c, t dbSNP:199588723
1530 1530 a, c, g dbSNP:201296209
1533 1533 a, c dbSNP:570769035
1535 1535 c, t dbSNP:749276702
1539 1539 a, g dbSNP:772079641
1540 1540 a, c dbSNP:539341562
1549 1549 c, t dbSNP:776584862
1551 1551 c, t dbSNP:200564104
1556 1556 c, t dbSNP:769604864
1562 1562 c, t dbSNP:200043004
1565 1565 a, c dbSNP:762579312
1568 1568 c, g dbSNP:763503185
1586 1586 -, tcctgca dbSNP:757614243
1588 1588 c, t dbSNP:773842361
1595 1595 c, t dbSNP:201784451
1596 1596 a, g dbSNP:767963054
1601 1601 a, g dbSNP:750815135
1614 1614 a, g dbSNP:144915500
1623 1623 a, c dbSNP:761087218
1631 1631 c, t dbSNP:766726017
1635 1635 a, g dbSNP:754165973
1657 1657 a, t dbSNP:138751864
1658 1658 a, g dbSNP:765455026
1662 1662 c, t dbSNP:148881618
1667 1667 a, g dbSNP:758525166
1669 1669 c, t dbSNP:781333856
1670 1670 a, g dbSNP:750379134
1673 1673 c, t dbSNP:143537324
1680 1680 c, t dbSNP:779723628
1685 1685 c, t dbSNP:749061350
1697 1697 a, g dbSNP:371696839
1709 1709 c, g dbSNP:778594593
1715 1715 a, g dbSNP:747704117
1722 1722 c, t dbSNP:771557532
1724 1724 c, t dbSNP:773777664
1725 1725 g, t dbSNP:200877443
1727 1727 a, g dbSNP:369621046
1736 1736 a, c dbSNP:771415207
1740 1740 c, t dbSNP:369066335
1741 1741 a, g dbSNP:759910002
1744 1744 c, t dbSNP:765421364
1748 1748 c, t dbSNP:550169210
1750 1750 a, g dbSNP:763189041
1754 1754 g, t dbSNP:764272389
1760 1760 c, t dbSNP:751628935
1761 1761 a, g dbSNP:756141971
1764 1764 a, c dbSNP:779999297
1774 1774 c, g dbSNP:753616111
1778 1778 c, t dbSNP:754778906
1786 1786 a, g dbSNP:574424212
1789 1789 a, g dbSNP:778489476
1794 1794 c, t dbSNP:374391699
1803 1803 c, t dbSNP:771647447
1813 1813 c, t dbSNP:777280375
1814 1814 a, g dbSNP:746476117
1825 1825 a, g dbSNP:771375798
1830 1830 c, t dbSNP:777169519
1831 1831 a, g dbSNP:144296616
1849 1849 c, t dbSNP:377757226
1874 1874 c, t dbSNP:187836713
1875 1875 c, t dbSNP:193231294
1876 1876 a, g dbSNP:772737565
1882 1882 c, t dbSNP:185303723
1889 1889 a, c dbSNP:778103184
1896 1896 c, t dbSNP:569611765
1909 1909 c, t dbSNP:529027937
1921 1921 c, t dbSNP:563133856
1925 1925 a, c dbSNP:190228579
1926 1926 g, t dbSNP:548961533
1947 1947 a, g dbSNP:746721057
1958 1958 c, t dbSNP:566088652
1970 1970 a, g dbSNP:148734093
2058 2058 c, t dbSNP:551738077
2076 2076 a, g dbSNP:571800369
2141 2141 c, t dbSNP:537530186
2149 2149 a, g dbSNP:557595465
2150 2150 c, t dbSNP:373000472
2158 2158 c, t dbSNP:142328283
2166 2166 c, t dbSNP:761185977
2170 2170 a, g dbSNP:567810998
2192 2192 a, g dbSNP:536972585
2198 2198 c, t dbSNP:565708040
2213 2213 c, t dbSNP:192811535
2257 2257 a, g dbSNP:540697823
2258 2258 c, t dbSNP:147831141
2259 2259 a, g dbSNP:552070370
2270 2270 -, g dbSNP:767813546
2278 2278 c, t dbSNP:577358612
2279 2279 a, g dbSNP:15506
2330 2330 c, t dbSNP:755269561
2331 2331 a, g dbSNP:543073118
2341 2341 a, g dbSNP:572524340
2345 2345 a, g dbSNP:141330096
2378 2378 c, t dbSNP:767571800

Target ORF information:

RefSeq Version XM_011536365
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011536365

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAAACAT TCAACGAGAA GGCTGAGCAG CTGGTGGAGA TTCTAGAAGC CAAGGCAGAT 
GGGCAGACCC CAGTGTCCAT GCAGGACATG CTGACCTACA CCGCCATGGA CATCCTGGCC
AAGGCAGCTT TTGGGATGGA GACCAGTATG CTGCTGGGTG CCCAGAAGCC TCTGTCCCAG
GCAGTGAAAC TTATGTTGGA GGGAATCACT GCGTCCCGCA ACACTCTGGC AAAGTTCCTG
CCAGGGAAGA GGAAGCAGCT CCGGGAGGTC CGGGAGAGCA TTCGCTTCCT GCGCCAGGTG
GGCAGGGACT GGGTCCAGCG CCGCCGGGAA GCCCTGAAGA GGGGCGAGGA GGTTCCTGCC
GACATCCTCA CACAGATTCT GAAAGCTGAA GAGGGAGCCC AGGACGACGA GGGTCTGCTG
GACAACTTCG TCACCTTCTT CATTGCTGGT CACGAGACCT CTGCCAACCA CTTGGCGTTC
ACAGTGATGG AGCTGTCTCG CCAGCCAGAG ATCGTGGCAA GGCTGCAGGC CGAGGTGGAT
GAGGTCATTG GTTCTAAGAG GTACCTGGAT TTCGAGGACC TGGGGAGACT GCAGTACCTG
TCCCAGGTCC TCAAAGAGTC GCTGAGGCTG TACCCACCAG CATGGGGCAC CTTTCGCCTG
CTGGAAGAGG AGACCTTGAT TGATGGGGTC AGAGTCCCCG GCAACACCCC GCTCTTGTTC
AGCACCTATG TCATGGGGCG GATGGACACA TACTTTGAGG ACCCGCTGAC TTTCAACCCC
GATCGCTTCG GCCCTGGAGC ACCCAAGCCA CGGTTCACCT ACTTCCCCTT CTCCCTGGGC
CACCGCTCCT GCATCGGGCA GCAGTTTGCT CAGATGGAGG TGAAGGTGGT CATGGCAAAG
CTGCTGCAGA GGCTGGAGTT CCGGCTGGTG CCCGGGCAGC GCTTCGGGCT GCAGGAGCAG
GCCACACTCA AGCCACTGGA CCCCGTGCTG TGCACCCTGC GGCCCCGCGG CTGGCAGCCC
GCACCCCCAC CACCCCCCTG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18783
Accession Version NM_006668.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1503bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product cholesterol 24-hydroxylase precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF094480.1. This sequence is a reference standard in the RefSeqGene project. Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF094480.1, BC022539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ACCGCGCTCG CAGCCGCTAC GAGCACATCC CCGGGCCGCC GCGGCCCAGT
TTCCTTCTAG GACACCTCCC CTGCTTTTGG AAAAAGGATG AGGTTGGTGG CCGTGTGCTC
CAAGATGTGT TTTTGGATTG GGCTAAGAAG TATGGACCTG TTGTGCGGGT CAACGTCTTC
CACAAAACCT CAGTCATCGT CACGAGTCCT GAGTCGGTTA AGAAGTTCCT GATGTCAACC
AAGTACAACA AGGACTCCAA GATGTACCGT GCGCTCCAGA CTGTGTTTGG TGAGAGACTC
TTCGGCCAAG GCTTGGTGTC CGAATGCAAC TATGAGCGCT GGCACAAGCA GCGGAGAGTC
ATAGACCTGG CCTTCAGCCG GAGCTCCTTG GTTAGCTTAA TGGAAACATT CAACGAGAAG
GCTGAGCAGC TGGTGGAGAT TCTAGAAGCC AAGGCAGATG GGCAGACCCC AGTGTCCATG
CAGGACATGC TGACCTACAC CGCCATGGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG
ACCAGTATGC TGCTGGGTGC CCAGAAGCCT CTGTCCCAGG CAGTGAAACT TATGTTGGAG
GGAATCACTG CGTCCCGCAA CACTCTGGCA AAGTTCCTGC CAGGGAAGAG GAAGCAGCTC
CGGGAGGTCC GGGAGAGCAT TCGCTTCCTG CGCCAGGTGG GCAGGGACTG GGTCCAGCGC
CGCCGGGAAG CCCTGAAGAG GGGCGAGGAG GTTCCTGCCG ACATCCTCAC ACAGATTCTG
AAAGCTGAAG AGGGAGCCCA GGACGACGAG GGTCTGCTGG ACAACTTCGT CACCTTCTTC
ATTGCTGGTC ACGAGACCTC TGCCAACCAC TTGGCGTTCA CAGTGATGGA GCTGTCTCGC
CAGCCAGAGA TCGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTCATTGG TTCTAAGAGG
TACCTGGATT TCGAGGACCT GGGGAGACTG CAGTACCTGT CCCAGGTCCT CAAAGAGTCG
CTGAGGCTGT ACCCACCAGC ATGGGGCACC TTTCGCCTGC TGGAAGAGGA GACCTTGATT
GATGGGGTCA GAGTCCCCGG CAACACCCCG CTCTTGTTCA GCACCTATGT CATGGGGCGG
ATGGACACAT ACTTTGAGGA CCCGCTGACT TTCAACCCCG ATCGCTTCGG CCCTGGAGCA
CCCAAGCCAC GGTTCACCTA CTTCCCCTTC TCCCTGGGCC ACCGCTCCTG CATCGGGCAG
CAGTTTGCTC AGATGGAGGT GAAGGTGGTC ATGGCAAAGC TGCTGCAGAG GCTGGAGTTC
CGGCTGGTGC CCGGGCAGCG CTTCGGGCTG CAGGAGCAGG CCACACTCAA GCCACTGGAC
CCCGTGCTGT GCACCCTGCG GCCCCGCGGC TGGCAGCCCG CACCCCCACC ACCCCCCTGC
TGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_006659.1
CDS1..1503
Misc Feature(1)7..69(+)
Misc Feature(2)100..1398(+)
Misc Feature(3)1309..1311(+)
Exon (1)1..119
Gene:CYP46A1
Gene Synonym:
Exon (2)120..200
Gene:CYP46A1
Gene Synonym:
Exon (3)201..282
Gene:CYP46A1
Gene Synonym:
Exon (4)283..356
Gene:CYP46A1
Gene Synonym:
Exon (5)357..443
Gene:CYP46A1
Gene Synonym:
Exon (6)444..582
Gene:CYP46A1
Gene Synonym:
Exon (7)583..693
Gene:CYP46A1
Gene Synonym:
Exon (8)694..844
Gene:CYP46A1
Gene Synonym:
Exon (9)845..907
Gene:CYP46A1
Gene Synonym:
Exon (10)908..980
Gene:CYP46A1
Gene Synonym:
Exon (11)981..1065
Gene:CYP46A1
Gene Synonym:
Exon (12)1066..1176
Gene:CYP46A1
Gene Synonym:
Exon (13)1177..1265
Gene:CYP46A1
Gene Synonym:
Exon (14)1266..1332
Gene:CYP46A1
Gene Synonym:
Exon (15)1333..2077
Gene:CYP46A1
Gene Synonym:
Translation
Position Chain Variation Link
48 48 a, c, t dbSNP:569975911
75 75 c, t dbSNP:779718133
105 105 a, g dbSNP:748912611
110 110 a, c dbSNP:768274775
122 122 a, t dbSNP:759885529
126 126 g, t dbSNP:769929751
153 153 a, g dbSNP:533477005
172 172 c, g dbSNP:762981146
173 173 a, c, g dbSNP:200569884
190 190 c, t dbSNP:760604778
210 210 g, t dbSNP:774423149
217 217 c, t dbSNP:761826761
219 219 c, t dbSNP:141032633
222 222 c, t dbSNP:556161085
227 227 a, g dbSNP:759291829
234 234 c, t dbSNP:765059068
235 235 a, g dbSNP:752350612
241 241 c, g dbSNP:760979832
243 243 c, g dbSNP:758126503
252 252 a, g dbSNP:763781486
258 258 a, c, t dbSNP:751135557
259 259 a, g dbSNP:781586799
266 266 g, t dbSNP:746361117
276 276 a, g dbSNP:756488741
277 277 a, g dbSNP:780458125
293 293 c, t dbSNP:755299289
306 306 a, c dbSNP:779147763
312 312 a, g dbSNP:748350226
327 327 a, c, t dbSNP:772101757
333 333 a, g dbSNP:747002018
342 342 -, tg dbSNP:749546833
342 342 c, t dbSNP:769792545
363 363 c, t dbSNP:780187016
378 378 g, t dbSNP:144966483
381 381 c, t dbSNP:139245529
382 382 a, g dbSNP:774289118
396 396 c, g dbSNP:761460288
423 423 a, g dbSNP:771783206
424 424 a, g dbSNP:772703431
439 439 c, t dbSNP:760275943
443 443 a, g dbSNP:765829489
449 449 c, t dbSNP:751871147
478 478 a, g dbSNP:75832974
483 483 a, t dbSNP:757555782
495 495 a, g dbSNP:767787355
504 504 a, g dbSNP:750556808
513 513 a, g dbSNP:756225751
523 523 a, c dbSNP:779038416
531 531 a, c dbSNP:748017615
536 536 a, c dbSNP:368496205
542 542 a, t dbSNP:777426743
550 550 c, t dbSNP:746753597
554 554 c, t dbSNP:770584624
558 558 c, t dbSNP:776224827
559 559 a, c, g dbSNP:74730047
562 562 a, g dbSNP:148568536
565 565 a, g dbSNP:776122077
585 585 a, g, t dbSNP:368295416
586 586 a, g dbSNP:745505656
588 588 c, t dbSNP:769357422
601 601 a, t dbSNP:779430731
613 613 c, t dbSNP:748771138
621 621 c, t dbSNP:769062361
624 624 g, t dbSNP:774884916
627 627 a, g dbSNP:762310846
629 629 c, t dbSNP:267604122
633 633 c, g dbSNP:372548812
635 635 c, t dbSNP:773630837
639 639 a, g dbSNP:760975591
653 653 c, t dbSNP:766557064
671 671 c, t dbSNP:753879253
672 672 a, g dbSNP:569614577
681 681 c, t dbSNP:763976882
683 683 c, g dbSNP:751475413
704 704 c, g dbSNP:771302947
713 713 a, t dbSNP:776775506
717 717 a, g dbSNP:746098686
722 722 a, g dbSNP:142905408
731 731 a, g dbSNP:775557276
743 743 a, g dbSNP:762967586
744 744 c, t dbSNP:767470135
751 751 c, t dbSNP:773100496
753 753 c, t dbSNP:760438576
755 755 a, g dbSNP:766185526
768 768 c, t dbSNP:753505149
774 774 c, g dbSNP:754668158
778 778 c, t dbSNP:764924761
779 779 a, c, g dbSNP:752278427
783 783 c, t dbSNP:778466149
785 785 a, g dbSNP:151175005
786 786 c, g dbSNP:757628267
789 789 a, g dbSNP:781623174
798 798 a, g dbSNP:746157910
804 804 c, t dbSNP:770021958
807 807 a, g dbSNP:780207418
818 818 c, t dbSNP:749407644
820 820 a, g dbSNP:768703295
823 823 a, g dbSNP:773194715
828 828 c, t dbSNP:201604068
840 840 a, g, t dbSNP:201085949
857 857 c, t dbSNP:775348328
858 858 c, t dbSNP:565548234
861 861 a, g, t dbSNP:532807703
864 864 c, t dbSNP:751098169
865 865 a, g dbSNP:761184361
867 867 c, t dbSNP:768106991
872 872 g, t dbSNP:750870929
873 873 a, t dbSNP:543441457
888 888 c, t dbSNP:780395313
889 889 a, g dbSNP:754138869
893 893 c, t dbSNP:755209726
896 896 c, t dbSNP:78767954
912 912 c, g, t dbSNP:200661407
930 930 c, t dbSNP:759997989
935 935 c, t dbSNP:765509152
940 940 a, g dbSNP:753035877
959 959 a, g dbSNP:758560402
960 960 c, t dbSNP:370474237
969 969 g, t dbSNP:112687989
972 972 c, t dbSNP:187491029
973 973 a, g dbSNP:757381529
975 975 a, g dbSNP:201760580
976 976 a, g dbSNP:780189156
978 978 a, g dbSNP:372949820
990 990 c, t dbSNP:188075791
1010 1010 a, g dbSNP:767699278
1017 1017 a, g dbSNP:750463063
1023 1023 c, t dbSNP:374987652
1032 1032 c, t dbSNP:754886094
1039 1039 c, t dbSNP:778862126
1048 1048 c, t dbSNP:368904176
1053 1053 a, g dbSNP:370665505
1059 1059 g, t dbSNP:758186359
1068 1068 c, g dbSNP:780483949
1074 1074 a, g dbSNP:749795671
1079 1079 c, t dbSNP:769217801
1081 1081 c, g dbSNP:774871671
1101 1101 a, g, t dbSNP:200110496
1109 1109 a, c dbSNP:773452402
1114 1114 c, t dbSNP:760720152
1115 1115 a, g dbSNP:766472276
1116 1116 c, g dbSNP:775360092
1119 1119 a, g dbSNP:762883900
1129 1129 a, g dbSNP:763967249
1131 1131 a, g, t dbSNP:751383710
1134 1134 c, t dbSNP:143992631
1143 1143 c, t dbSNP:750076915
1155 1155 c, g dbSNP:755645477
1158 1158 c, t dbSNP:779618668
1159 1159 a, g dbSNP:146435738
1164 1164 c, t dbSNP:755567035
1168 1168 c, t dbSNP:779523894
1169 1169 c, t dbSNP:748564064
1170 1170 a, g dbSNP:140794624
1173 1173 c, g dbSNP:550352631
1182 1182 c, t dbSNP:773079915
1185 1185 a, c dbSNP:760348654
1191 1191 c, t dbSNP:766097065
1204 1204 c, g dbSNP:568187423
1210 1210 c, t dbSNP:749206215
1219 1219 a, g dbSNP:764838844
1221 1221 c, t dbSNP:375146687
1224 1224 a, g dbSNP:369668371
1229 1229 a, c dbSNP:778095376
1236 1236 c, t dbSNP:751991710
1239 1239 c, t dbSNP:757537926
1240 1240 a, g dbSNP:557127125
1248 1248 c, t dbSNP:199588723
1249 1249 a, c, g dbSNP:201296209
1252 1252 a, c dbSNP:570769035
1254 1254 c, t dbSNP:749276702
1258 1258 a, g dbSNP:772079641
1259 1259 a, c dbSNP:539341562
1268 1268 c, t dbSNP:776584862
1270 1270 c, t dbSNP:200564104
1275 1275 c, t dbSNP:769604864
1281 1281 c, t dbSNP:200043004
1284 1284 a, c dbSNP:762579312
1287 1287 c, g dbSNP:763503185
1305 1305 -, tcctgca dbSNP:757614243
1307 1307 c, t dbSNP:773842361
1314 1314 c, t dbSNP:201784451
1315 1315 a, g dbSNP:767963054
1320 1320 a, g dbSNP:750815135
1333 1333 a, g dbSNP:144915500
1342 1342 a, c dbSNP:761087218
1350 1350 c, t dbSNP:766726017
1354 1354 a, g dbSNP:754165973
1376 1376 a, t dbSNP:138751864
1377 1377 a, g dbSNP:765455026
1381 1381 c, t dbSNP:148881618
1386 1386 a, g dbSNP:758525166
1388 1388 c, t dbSNP:781333856
1389 1389 a, g dbSNP:750379134
1392 1392 c, t dbSNP:143537324
1399 1399 c, t dbSNP:779723628
1404 1404 c, t dbSNP:749061350
1416 1416 a, g dbSNP:371696839
1428 1428 c, g dbSNP:778594593
1434 1434 a, g dbSNP:747704117
1441 1441 c, t dbSNP:771557532
1443 1443 c, t dbSNP:773777664
1444 1444 g, t dbSNP:200877443
1446 1446 a, g dbSNP:369621046
1455 1455 a, c dbSNP:771415207
1459 1459 c, t dbSNP:369066335
1460 1460 a, g dbSNP:759910002
1463 1463 c, t dbSNP:765421364
1467 1467 c, t dbSNP:550169210
1469 1469 a, g dbSNP:763189041
1473 1473 g, t dbSNP:764272389
1479 1479 c, t dbSNP:751628935
1480 1480 a, g dbSNP:756141971
1483 1483 a, c dbSNP:779999297
1493 1493 c, g dbSNP:753616111
1497 1497 c, t dbSNP:754778906
1505 1505 a, g dbSNP:574424212
1508 1508 a, g dbSNP:778489476
1513 1513 c, t dbSNP:374391699
1522 1522 c, t dbSNP:771647447
1532 1532 c, t dbSNP:777280375
1533 1533 a, g dbSNP:746476117
1544 1544 a, g dbSNP:771375798
1549 1549 c, t dbSNP:777169519
1550 1550 a, g dbSNP:144296616
1568 1568 c, t dbSNP:377757226
1593 1593 c, t dbSNP:187836713
1594 1594 c, t dbSNP:193231294
1595 1595 a, g dbSNP:772737565
1601 1601 c, t dbSNP:185303723
1608 1608 a, c dbSNP:778103184
1615 1615 c, t dbSNP:569611765
1628 1628 c, t dbSNP:529027937
1640 1640 c, t dbSNP:563133856
1644 1644 a, c dbSNP:190228579
1645 1645 g, t dbSNP:548961533
1666 1666 a, g dbSNP:746721057
1677 1677 c, t dbSNP:566088652
1689 1689 a, g dbSNP:148734093
1777 1777 c, t dbSNP:551738077
1795 1795 a, g dbSNP:571800369
1860 1860 c, t dbSNP:537530186
1868 1868 a, g dbSNP:557595465
1869 1869 c, t dbSNP:373000472
1877 1877 c, t dbSNP:142328283
1885 1885 c, t dbSNP:761185977
1889 1889 a, g dbSNP:567810998
1911 1911 a, g dbSNP:536972585
1917 1917 c, t dbSNP:565708040
1932 1932 c, t dbSNP:192811535
1976 1976 a, g dbSNP:540697823
1977 1977 c, t dbSNP:147831141
1978 1978 a, g dbSNP:552070370
1989 1989 -, g dbSNP:767813546
1997 1997 c, t dbSNP:577358612
1998 1998 a, g dbSNP:15506
2049 2049 c, t dbSNP:755269561
2050 2050 a, g dbSNP:543073118
2060 2060 a, g dbSNP:572524340
2064 2064 a, g dbSNP:141330096

Target ORF information:

RefSeq Version NM_006668
Organism Homo sapiens (human)
Definition Homo sapiens cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006668

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ACCGCGCTCG CAGCCGCTAC GAGCACATCC CCGGGCCGCC GCGGCCCAGT
TTCCTTCTAG GACACCTCCC CTGCTTTTGG AAAAAGGATG AGGTTGGTGG CCGTGTGCTC
CAAGATGTGT TTTTGGATTG GGCTAAGAAG TATGGACCTG TTGTGCGGGT CAACGTCTTC
CACAAAACCT CAGTCATCGT CACGAGTCCT GAGTCGGTTA AGAAGTTCCT GATGTCAACC
AAGTACAACA AGGACTCCAA GATGTACCGT GCGCTCCAGA CTGTGTTTGG TGAGAGACTC
TTCGGCCAAG GCTTGGTGTC CGAATGCAAC TATGAGCGCT GGCACAAGCA GCGGAGAGTC
ATAGACCTGG CCTTCAGCCG GAGCTCCTTG GTTAGCTTAA TGGAAACATT CAACGAGAAG
GCTGAGCAGC TGGTGGAGAT TCTAGAAGCC AAGGCAGATG GGCAGACCCC AGTGTCCATG
CAGGACATGC TGACCTACAC CGCCATGGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG
ACCAGTATGC TGCTGGGTGC CCAGAAGCCT CTGTCCCAGG CAGTGAAACT TATGTTGGAG
GGAATCACTG CGTCCCGCAA CACTCTGGCA AAGTTCCTGC CAGGGAAGAG GAAGCAGCTC
CGGGAGGTCC GGGAGAGCAT TCGCTTCCTG CGCCAGGTGG GCAGGGACTG GGTCCAGCGC
CGCCGGGAAG CCCTGAAGAG GGGCGAGGAG GTTCCTGCCG ACATCCTCAC ACAGATTCTG
AAAGCTGAAG AGGGAGCCCA GGACGACGAG GGTCTGCTGG ACAACTTCGT CACCTTCTTC
ATTGCTGGTC ACGAGACCTC TGCCAACCAC TTGGCGTTCA CAGTGATGGA GCTGTCTCGC
CAGCCAGAGA TCGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTCATTGG TTCTAAGAGG
TACCTGGATT TCGAGGACCT GGGGAGACTG CAGTACCTGT CCCAGGTCCT CAAAGAGTCG
CTGAGGCTGT ACCCACCAGC ATGGGGCACC TTTCGCCTGC TGGAAGAGGA GACCTTGATT
GATGGGGTCA GAGTCCCCGG CAACACCCCG CTCTTGTTCA GCACCTATGT CATGGGGCGG
ATGGACACAT ACTTTGAGGA CCCGCTGACT TTCAACCCCG ATCGCTTCGG CCCTGGAGCA
CCCAAGCCAC GGTTCACCTA CTTCCCCTTC TCCCTGGGCC ACCGCTCCTG CATCGGGCAG
CAGTTTGCTC AGATGGAGGT GAAGGTGGTC ATGGCAAAGC TGCTGCAGAG GCTGGAGTTC
CGGCTGGTGC CCGGGCAGCG CTTCGGGCTG CAGGAGCAGG CCACACTCAA GCCACTGGAC
CCCGTGCTGT GCACCCTGCG GCCCCGCGGC TGGCAGCCCG CACCCCCACC ACCCCCCTGC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.