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MAGED2 melanoma antigen family D2 [Homo sapiens (human)]

Gene Symbol MAGED2
Entrez Gene ID 10916
Full Name melanoma antigen family D2
Synonyms 11B6, BCG-1, BCG1, HCA10, MAGE-D2
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp11.2

Summary This gene is a member of the MAGED gene family. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR). This gene may also be involved in several types of cancer, including breast cancer and melanoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]. lac of sum
Disorder MIM:

300470

Disorder Html:





The following MAGED2 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGED2 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu02833 NM_177433 Homo sapiens melanoma antigen family D2 (MAGED2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $379
OHu02833 NM_201222 Homo sapiens melanoma antigen family D2 (MAGED2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $379
OHu02833 NM_014599 Homo sapiens melanoma antigen family D2 (MAGED2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $379

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu02833
Accession Version NM_177433.2
Sequence Information ORF Nucleotide Sequence (Length: 1821bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB467873.1, BC000304.2 and BX647995.1. This sequence is a reference standard in the RefSeqGene project. On Aug 20, 2013 this sequence version replaced gi:29171704. Summary: This gene is a member of the MAGED gene family. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR). This gene may also be involved in several types of cancer, including breast cancer and melanoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]. Transcript Variant: This variant (2) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC091503.1, U92544.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC
TGTGGTTTCT CCTACAAGTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_803182.1
CDS234..2054
Misc Feature(1)228..230(+)
Misc Feature(2)237..239(+)
Misc Feature(3)237..239(+)
Misc Feature(4)243..245(+)
Misc Feature(5)246..248(+)
Misc Feature(6)252..254(+)
Misc Feature(7)432..434(+)
Misc Feature(8)447..449(+)
Misc Feature(9)669..671(+)
Misc Feature(10)702..704(+)
Misc Feature(11)702..704(+)
Misc Feature(12)717..719(+)
Misc Feature(13)801..803(+)
Misc Feature(14)801..803(+)
Misc Feature(15)804..806(+)
Misc Feature(16)804..806(+)
Misc Feature(17)813..815(+)
Misc Feature(18)813..815(+)
Misc Feature(19)822..824(+)
Misc Feature(20)963..965(+)
Misc Feature(21)972..974(+)
Misc Feature(22)972..974(+)
Misc Feature(23)1026..1028(+)
Misc Feature(24)1026..1028(+)
Misc Feature(25)1089..1601(+)
Exon (1)1..204
Gene:MAGED2
Gene Synonym:
Exon (2)205..278
Gene:MAGED2
Gene Synonym:
Exon (3)279..770
Gene:MAGED2
Gene Synonym:
Exon (4)771..1079
Gene:MAGED2
Gene Synonym:
Exon (5)1080..1143
Gene:MAGED2
Gene Synonym:
Exon (6)1144..1223
Gene:MAGED2
Gene Synonym:
Exon (7)1224..1318
Gene:MAGED2
Gene Synonym:
Exon (8)1319..1398
Gene:MAGED2
Gene Synonym:
Exon (9)1399..1441
Gene:MAGED2
Gene Synonym:
Exon (10)1442..1504
Gene:MAGED2
Gene Synonym:
Exon (11)1505..1619
Gene:MAGED2
Gene Synonym:
Exon (12)1620..2062
Gene:MAGED2
Gene Synonym:
Exon (13)2063..2197
Gene:MAGED2
Gene Synonym:
Translation
Position Chain Variation Link
7 7 a, g dbSNP:755884021
44 44 g, t dbSNP:764079560
63 63 c, t dbSNP:753774232
66 66 -, g dbSNP:34737456
74 74 -, g dbSNP:756768683
76 76 g, t dbSNP:755132018
77 77 c, g dbSNP:373693407
202 202 a, g dbSNP:1127034
205 205 a, g dbSNP:202084450
224 224 a, c, t dbSNP:372889574
227 227 c, t dbSNP:765553866
244 244 c, g dbSNP:750624649
263 263 a, t dbSNP:111329495
272 272 c, t dbSNP:11555929
299 299 c, t dbSNP:770665474
307 307 c, t dbSNP:774057511
312 312 a, g dbSNP:200706314
313 313 c, t dbSNP:769357290
325 325 g, t dbSNP:147495006
350 350 c, g dbSNP:762323814
361 361 c, t dbSNP:201160051
362 362 a, g dbSNP:140063708
363 363 a, g dbSNP:11555928
377 377 a, g dbSNP:11555932
398 398 c, g dbSNP:766564460
420 420 a, g dbSNP:751676539
429 429 c, g dbSNP:11555930
437 437 a, g dbSNP:755046012
457 457 c, g dbSNP:781247578
458 458 c, t dbSNP:144346983
459 459 c, t dbSNP:767820484
460 460 a, g dbSNP:148790335
468 468 c, t dbSNP:777456577
475 475 a, c, t dbSNP:749072773
479 479 g, t dbSNP:778707488
484 484 c, t dbSNP:745432175
485 485 a, g dbSNP:1043031
497 497 g, t dbSNP:6614290
523 523 c, g dbSNP:760829080
539 539 a, g dbSNP:150921056
550 550 c, g dbSNP:748635209
552 552 a, c dbSNP:770287312
569 569 c, t dbSNP:773614218
571 571 c, t dbSNP:763271575
572 572 a, g dbSNP:766654234
574 574 a, g dbSNP:185932631
576 576 c, g dbSNP:759692914
577 577 a, c, t dbSNP:371162915
593 593 c, t dbSNP:752778394
608 608 a, g dbSNP:756025911
609 609 g, t dbSNP:764063667
624 624 a, g dbSNP:753756584
628 628 c, t dbSNP:757108375
629 629 a, g dbSNP:1043034
640 640 c, t dbSNP:745521206
678 678 c, t dbSNP:758029411
682 682 a, c dbSNP:779453522
690 690 c, t dbSNP:746508104
697 697 c, t dbSNP:770241601
701 701 a, g dbSNP:200476948
713 713 c, t dbSNP:138895309
717 717 g, t dbSNP:372980079
731 731 c, g dbSNP:376206910
743 743 c, t dbSNP:774627072
744 744 a, c dbSNP:759704782
765 765 c, g dbSNP:767728271
766 766 a, g dbSNP:775474912
787 787 c, g dbSNP:758038664
794 794 c, g dbSNP:12014977
796 796 a, g dbSNP:751096381
807 807 a, g dbSNP:754497190
813 813 a, c dbSNP:780622232
817 817 a, g dbSNP:749796098
833 833 a, c dbSNP:771383792
841 841 a, g dbSNP:779268605
848 848 c, t dbSNP:370589511
857 857 c, t dbSNP:1043044
866 866 c, t dbSNP:775770634
890 890 a, g dbSNP:760754361
910 910 c, t dbSNP:768866249
913 913 a, g dbSNP:199838183
917 917 a, g dbSNP:761865238
918 918 g, t dbSNP:765245524
925 925 a, g dbSNP:750226910
928 928 c, g dbSNP:762802111
938 938 g, t dbSNP:766003948
939 939 c, g dbSNP:751237092
948 948 c, t dbSNP:754520173
962 962 c, t dbSNP:780707837
985 985 g, t dbSNP:752150960
986 986 c, t dbSNP:757807028
992 992 c, t dbSNP:779390979
999 999 c, t dbSNP:746278493
1001 1001 c, t dbSNP:772502489
1003 1003 a, g dbSNP:368156326
1008 1008 a, g dbSNP:780220478
1032 1032 c, g dbSNP:747287337
1049 1049 a, g dbSNP:111542041
1054 1054 c, g dbSNP:776901422
1117 1117 a, g dbSNP:372339836
1120 1120 c, t dbSNP:138036392
1125 1125 a, g dbSNP:755292405
1126 1126 c, t dbSNP:781562704
1130 1130 c, t dbSNP:749078330
1142 1142 a, g dbSNP:771039734
1145 1145 c, t dbSNP:748382114
1174 1174 a, c dbSNP:756360755
1175 1175 a, t dbSNP:777930279
1185 1185 c, t dbSNP:749305354
1187 1187 c, t dbSNP:149100572
1214 1214 c, t dbSNP:2071932
1220 1220 a, g dbSNP:745749102
1238 1238 a, g dbSNP:768020894
1239 1239 c, t dbSNP:191668283
1260 1260 c, g dbSNP:756408108
1278 1278 c, g dbSNP:184999100
1281 1281 a, g dbSNP:781549172
1296 1296 a, g dbSNP:139029259
1311 1311 c, t dbSNP:372304202
1325 1325 a, g dbSNP:762010356
1334 1334 c, g dbSNP:761467957
1358 1358 c, t dbSNP:769188952
1370 1370 c, t dbSNP:143026645
1386 1386 a, c, t dbSNP:147480000
1398 1398 g, t dbSNP:768115012
1424 1424 a, g dbSNP:138363965
1434 1434 c, t dbSNP:746882460
1445 1445 a, g dbSNP:141904298
1466 1466 c, t dbSNP:371362791
1471 1471 a, g dbSNP:758463316
1482 1482 a, c dbSNP:766390320
1526 1526 c, t dbSNP:753666567
1531 1531 a, g dbSNP:375724669
1537 1537 a, g dbSNP:778624859
1539 1539 c, t dbSNP:369099604
1541 1541 a, c dbSNP:757943408
1579 1579 a, g dbSNP:780966437
1601 1601 c, t dbSNP:150655488
1637 1637 c, t dbSNP:760744777
1647 1647 a, g dbSNP:764094559
1658 1658 c, t dbSNP:753752812
1660 1660 a, g dbSNP:765344449
1664 1664 a, g dbSNP:751416614
1676 1676 a, g dbSNP:11555927
1686 1686 g, t dbSNP:757951021
1695 1695 a, g dbSNP:779634966
1697 1697 a, g dbSNP:767508861
1699 1699 c, t dbSNP:756650378
1724 1724 c, t dbSNP:778245642
1738 1738 a, g dbSNP:749710147
1754 1754 c, t dbSNP:370758368
1760 1760 a, g dbSNP:779078378
1772 1772 c, t dbSNP:746132056
1778 1778 c, t dbSNP:149849605
1790 1790 c, t dbSNP:775755120
1802 1802 c, t dbSNP:747081998
1814 1814 c, t dbSNP:146407393
1823 1823 g, t dbSNP:752562003
1828 1828 a, g dbSNP:761593066
1832 1832 a, g dbSNP:139801905
1864 1864 g, t dbSNP:1804969
1882 1882 -, gtgcca dbSNP:752649471
1891 1891 c, g dbSNP:143128713
1900 1900 a, g dbSNP:762712208
1902 1902 -, aac dbSNP:758402011
1903 1903 a, g dbSNP:765982882
1904 1904 -, cagtgc dbSNP:777217330
1912 1912 a, g dbSNP:751132389
1916 1916 c, t dbSNP:754468012
1920 1920 -, gccagc dbSNP:745964344
1920 1920 a, g dbSNP:763080639
1924 1924 a, g dbSNP:766954953
1928 1928 c, t dbSNP:754347423
1937 1937 c, t dbSNP:1804968
1941 1941 a, g dbSNP:757663000
1958 1958 a, g dbSNP:11555934
1961 1961 c, t dbSNP:375313111
1962 1962 a, g dbSNP:376899197
1967 1967 g, t dbSNP:758716770
1986 1986 c, g, t dbSNP:200491129
1992 1992 c, t dbSNP:747171863
2001 2001 c, g dbSNP:768864626
2042 2042 c, t dbSNP:776815032
2075 2075 a, c dbSNP:1804967
2191 2191 a, t dbSNP:111765517
2194 2194 c, t dbSNP:377424961

Target ORF information:

RefSeq Version NM_177433
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D2 (MAGED2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_177433

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02833
Accession Version NM_201222.2
Sequence Information ORF Nucleotide Sequence (Length: 1821bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM043994.1, BC000304.2 and BX647995.1. On Aug 20, 2013 this sequence version replaced gi:41350319. Summary: This gene is a member of the MAGED gene family. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR). This gene may also be involved in several types of cancer, including breast cancer and melanoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. Variants 1, 2, and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BX647995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC
TGTGGTTTCT CCTACAAGTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_957516.1
CDS194..2014
Misc Feature(1)188..190(+)
Misc Feature(2)197..199(+)
Misc Feature(3)197..199(+)
Misc Feature(4)203..205(+)
Misc Feature(5)206..208(+)
Misc Feature(6)212..214(+)
Misc Feature(7)392..394(+)
Misc Feature(8)407..409(+)
Misc Feature(9)629..631(+)
Misc Feature(10)662..664(+)
Misc Feature(11)662..664(+)
Misc Feature(12)677..679(+)
Misc Feature(13)761..763(+)
Misc Feature(14)761..763(+)
Misc Feature(15)764..766(+)
Misc Feature(16)764..766(+)
Misc Feature(17)773..775(+)
Misc Feature(18)773..775(+)
Misc Feature(19)782..784(+)
Misc Feature(20)923..925(+)
Misc Feature(21)932..934(+)
Misc Feature(22)932..934(+)
Misc Feature(23)986..988(+)
Misc Feature(24)986..988(+)
Misc Feature(25)1049..1561(+)
Exon (1)1..164
Gene:MAGED2
Gene Synonym:
Exon (2)165..238
Gene:MAGED2
Gene Synonym:
Exon (3)239..730
Gene:MAGED2
Gene Synonym:
Exon (4)731..1039
Gene:MAGED2
Gene Synonym:
Exon (5)1040..1103
Gene:MAGED2
Gene Synonym:
Exon (6)1104..1183
Gene:MAGED2
Gene Synonym:
Exon (7)1184..1278
Gene:MAGED2
Gene Synonym:
Exon (8)1279..1358
Gene:MAGED2
Gene Synonym:
Exon (9)1359..1401
Gene:MAGED2
Gene Synonym:
Exon (10)1402..1464
Gene:MAGED2
Gene Synonym:
Exon (11)1465..1579
Gene:MAGED2
Gene Synonym:
Exon (12)1580..2022
Gene:MAGED2
Gene Synonym:
Exon (13)2023..2157
Gene:MAGED2
Gene Synonym:
Translation
Position Chain Variation Link
29 29 c, t dbSNP:768465931
36 36 a, g dbSNP:776374479
38 38 c, t dbSNP:761567484
119 119 g, t dbSNP:566459130
165 165 a, g dbSNP:202084450
184 184 a, c, t dbSNP:372889574
187 187 c, t dbSNP:765553866
204 204 c, g dbSNP:750624649
223 223 a, t dbSNP:111329495
232 232 c, t dbSNP:11555929
259 259 c, t dbSNP:770665474
267 267 c, t dbSNP:774057511
272 272 a, g dbSNP:200706314
273 273 c, t dbSNP:769357290
285 285 g, t dbSNP:147495006
310 310 c, g dbSNP:762323814
321 321 c, t dbSNP:201160051
322 322 a, g dbSNP:140063708
323 323 a, g dbSNP:11555928
337 337 a, g dbSNP:11555932
358 358 c, g dbSNP:766564460
380 380 a, g dbSNP:751676539
389 389 c, g dbSNP:11555930
397 397 a, g dbSNP:755046012
417 417 c, g dbSNP:781247578
418 418 c, t dbSNP:144346983
419 419 c, t dbSNP:767820484
420 420 a, g dbSNP:148790335
428 428 c, t dbSNP:777456577
435 435 a, c, t dbSNP:749072773
439 439 g, t dbSNP:778707488
444 444 c, t dbSNP:745432175
445 445 a, g dbSNP:1043031
457 457 g, t dbSNP:6614290
483 483 c, g dbSNP:760829080
499 499 a, g dbSNP:150921056
510 510 c, g dbSNP:748635209
512 512 a, c dbSNP:770287312
529 529 c, t dbSNP:773614218
531 531 c, t dbSNP:763271575
532 532 a, g dbSNP:766654234
534 534 a, g dbSNP:185932631
536 536 c, g dbSNP:759692914
537 537 a, c, t dbSNP:371162915
553 553 c, t dbSNP:752778394
568 568 a, g dbSNP:756025911
569 569 g, t dbSNP:764063667
584 584 a, g dbSNP:753756584
588 588 c, t dbSNP:757108375
589 589 a, g dbSNP:1043034
600 600 c, t dbSNP:745521206
638 638 c, t dbSNP:758029411
642 642 a, c dbSNP:779453522
650 650 c, t dbSNP:746508104
657 657 c, t dbSNP:770241601
661 661 a, g dbSNP:200476948
673 673 c, t dbSNP:138895309
677 677 g, t dbSNP:372980079
691 691 c, g dbSNP:376206910
703 703 c, t dbSNP:774627072
704 704 a, c dbSNP:759704782
725 725 c, g dbSNP:767728271
726 726 a, g dbSNP:775474912
747 747 c, g dbSNP:758038664
754 754 c, g dbSNP:12014977
756 756 a, g dbSNP:751096381
767 767 a, g dbSNP:754497190
773 773 a, c dbSNP:780622232
777 777 a, g dbSNP:749796098
793 793 a, c dbSNP:771383792
801 801 a, g dbSNP:779268605
808 808 c, t dbSNP:370589511
817 817 c, t dbSNP:1043044
826 826 c, t dbSNP:775770634
850 850 a, g dbSNP:760754361
870 870 c, t dbSNP:768866249
873 873 a, g dbSNP:199838183
877 877 a, g dbSNP:761865238
878 878 g, t dbSNP:765245524
885 885 a, g dbSNP:750226910
888 888 c, g dbSNP:762802111
898 898 g, t dbSNP:766003948
899 899 c, g dbSNP:751237092
908 908 c, t dbSNP:754520173
922 922 c, t dbSNP:780707837
945 945 g, t dbSNP:752150960
946 946 c, t dbSNP:757807028
952 952 c, t dbSNP:779390979
959 959 c, t dbSNP:746278493
961 961 c, t dbSNP:772502489
963 963 a, g dbSNP:368156326
968 968 a, g dbSNP:780220478
992 992 c, g dbSNP:747287337
1009 1009 a, g dbSNP:111542041
1014 1014 c, g dbSNP:776901422
1077 1077 a, g dbSNP:372339836
1080 1080 c, t dbSNP:138036392
1085 1085 a, g dbSNP:755292405
1086 1086 c, t dbSNP:781562704
1090 1090 c, t dbSNP:749078330
1102 1102 a, g dbSNP:771039734
1105 1105 c, t dbSNP:748382114
1134 1134 a, c dbSNP:756360755
1135 1135 a, t dbSNP:777930279
1145 1145 c, t dbSNP:749305354
1147 1147 c, t dbSNP:149100572
1174 1174 c, t dbSNP:2071932
1180 1180 a, g dbSNP:745749102
1198 1198 a, g dbSNP:768020894
1199 1199 c, t dbSNP:191668283
1220 1220 c, g dbSNP:756408108
1238 1238 c, g dbSNP:184999100
1241 1241 a, g dbSNP:781549172
1256 1256 a, g dbSNP:139029259
1271 1271 c, t dbSNP:372304202
1285 1285 a, g dbSNP:762010356
1294 1294 c, g dbSNP:761467957
1318 1318 c, t dbSNP:769188952
1330 1330 c, t dbSNP:143026645
1346 1346 a, c, t dbSNP:147480000
1358 1358 g, t dbSNP:768115012
1384 1384 a, g dbSNP:138363965
1394 1394 c, t dbSNP:746882460
1405 1405 a, g dbSNP:141904298
1426 1426 c, t dbSNP:371362791
1431 1431 a, g dbSNP:758463316
1442 1442 a, c dbSNP:766390320
1486 1486 c, t dbSNP:753666567
1491 1491 a, g dbSNP:375724669
1497 1497 a, g dbSNP:778624859
1499 1499 c, t dbSNP:369099604
1501 1501 a, c dbSNP:757943408
1539 1539 a, g dbSNP:780966437
1561 1561 c, t dbSNP:150655488
1597 1597 c, t dbSNP:760744777
1607 1607 a, g dbSNP:764094559
1618 1618 c, t dbSNP:753752812
1620 1620 a, g dbSNP:765344449
1624 1624 a, g dbSNP:751416614
1636 1636 a, g dbSNP:11555927
1646 1646 g, t dbSNP:757951021
1655 1655 a, g dbSNP:779634966
1657 1657 a, g dbSNP:767508861
1659 1659 c, t dbSNP:756650378
1684 1684 c, t dbSNP:778245642
1698 1698 a, g dbSNP:749710147
1714 1714 c, t dbSNP:370758368
1720 1720 a, g dbSNP:779078378
1732 1732 c, t dbSNP:746132056
1738 1738 c, t dbSNP:149849605
1750 1750 c, t dbSNP:775755120
1762 1762 c, t dbSNP:747081998
1774 1774 c, t dbSNP:146407393
1783 1783 g, t dbSNP:752562003
1788 1788 a, g dbSNP:761593066
1792 1792 a, g dbSNP:139801905
1824 1824 g, t dbSNP:1804969
1842 1842 -, gtgcca dbSNP:752649471
1851 1851 c, g dbSNP:143128713
1860 1860 a, g dbSNP:762712208
1862 1862 -, aac dbSNP:758402011
1863 1863 a, g dbSNP:765982882
1864 1864 -, cagtgc dbSNP:777217330
1872 1872 a, g dbSNP:751132389
1876 1876 c, t dbSNP:754468012
1880 1880 -, gccagc dbSNP:745964344
1880 1880 a, g dbSNP:763080639
1884 1884 a, g dbSNP:766954953
1888 1888 c, t dbSNP:754347423
1897 1897 c, t dbSNP:1804968
1901 1901 a, g dbSNP:757663000
1918 1918 a, g dbSNP:11555934
1921 1921 c, t dbSNP:375313111
1922 1922 a, g dbSNP:376899197
1927 1927 g, t dbSNP:758716770
1946 1946 c, g, t dbSNP:200491129
1952 1952 c, t dbSNP:747171863
1961 1961 c, g dbSNP:768864626
2002 2002 c, t dbSNP:776815032
2035 2035 a, c dbSNP:1804967
2151 2151 a, t dbSNP:111765517
2154 2154 c, t dbSNP:377424961

Target ORF information:

RefSeq Version NM_201222
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D2 (MAGED2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_201222

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02833
Accession Version NM_014599.5
Sequence Information ORF Nucleotide Sequence (Length: 1821bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z98046.1, HY017171.1, BC000304.2 and BX647995.1. On Aug 20, 2013 this sequence version replaced gi:29171703. Summary: This gene is a member of the MAGED gene family. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR). This gene may also be involved in several types of cancer, including breast cancer and melanoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1, 2, and 3 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BC000304.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC
TGTGGTTTCT CCTACAAGTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_055414.2
CDS126..1946
Misc Feature(1)120..122(+)
Misc Feature(2)129..131(+)
Misc Feature(3)129..131(+)
Misc Feature(4)135..137(+)
Misc Feature(5)138..140(+)
Misc Feature(6)144..146(+)
Misc Feature(7)324..326(+)
Misc Feature(8)339..341(+)
Misc Feature(9)561..563(+)
Misc Feature(10)594..596(+)
Misc Feature(11)594..596(+)
Misc Feature(12)609..611(+)
Misc Feature(13)693..695(+)
Misc Feature(14)693..695(+)
Misc Feature(15)696..698(+)
Misc Feature(16)696..698(+)
Misc Feature(17)705..707(+)
Misc Feature(18)705..707(+)
Misc Feature(19)714..716(+)
Misc Feature(20)855..857(+)
Misc Feature(21)864..866(+)
Misc Feature(22)864..866(+)
Misc Feature(23)918..920(+)
Misc Feature(24)918..920(+)
Misc Feature(25)981..1493(+)
Exon (1)1..96
Gene:MAGED2
Gene Synonym:
Exon (2)97..170
Gene:MAGED2
Gene Synonym:
Exon (3)171..662
Gene:MAGED2
Gene Synonym:
Exon (4)663..971
Gene:MAGED2
Gene Synonym:
Exon (5)972..1035
Gene:MAGED2
Gene Synonym:
Exon (6)1036..1115
Gene:MAGED2
Gene Synonym:
Exon (7)1116..1210
Gene:MAGED2
Gene Synonym:
Exon (8)1211..1290
Gene:MAGED2
Gene Synonym:
Exon (9)1291..1333
Gene:MAGED2
Gene Synonym:
Exon (10)1334..1396
Gene:MAGED2
Gene Synonym:
Exon (11)1397..1511
Gene:MAGED2
Gene Synonym:
Exon (12)1512..1954
Gene:MAGED2
Gene Synonym:
Exon (13)1955..2089
Gene:MAGED2
Gene Synonym:
Translation
Position Chain Variation Link
54 54 c, g dbSNP:771235639
62 62 a, t dbSNP:774426541
64 64 a, c dbSNP:758297980
75 75 c, t dbSNP:11555931
97 97 a, g dbSNP:202084450
116 116 a, c, t dbSNP:372889574
119 119 c, t dbSNP:765553866
136 136 c, g dbSNP:750624649
155 155 a, t dbSNP:111329495
164 164 c, t dbSNP:11555929
191 191 c, t dbSNP:770665474
199 199 c, t dbSNP:774057511
204 204 a, g dbSNP:200706314
205 205 c, t dbSNP:769357290
217 217 g, t dbSNP:147495006
242 242 c, g dbSNP:762323814
253 253 c, t dbSNP:201160051
254 254 a, g dbSNP:140063708
255 255 a, g dbSNP:11555928
269 269 a, g dbSNP:11555932
290 290 c, g dbSNP:766564460
312 312 a, g dbSNP:751676539
321 321 c, g dbSNP:11555930
329 329 a, g dbSNP:755046012
349 349 c, g dbSNP:781247578
350 350 c, t dbSNP:144346983
351 351 c, t dbSNP:767820484
352 352 a, g dbSNP:148790335
360 360 c, t dbSNP:777456577
367 367 a, c, t dbSNP:749072773
371 371 g, t dbSNP:778707488
376 376 c, t dbSNP:745432175
377 377 a, g dbSNP:1043031
389 389 g, t dbSNP:6614290
415 415 c, g dbSNP:760829080
431 431 a, g dbSNP:150921056
442 442 c, g dbSNP:748635209
444 444 a, c dbSNP:770287312
461 461 c, t dbSNP:773614218
463 463 c, t dbSNP:763271575
464 464 a, g dbSNP:766654234
466 466 a, g dbSNP:185932631
468 468 c, g dbSNP:759692914
469 469 a, c, t dbSNP:371162915
485 485 c, t dbSNP:752778394
500 500 a, g dbSNP:756025911
501 501 g, t dbSNP:764063667
516 516 a, g dbSNP:753756584
520 520 c, t dbSNP:757108375
521 521 a, g dbSNP:1043034
532 532 c, t dbSNP:745521206
570 570 c, t dbSNP:758029411
574 574 a, c dbSNP:779453522
582 582 c, t dbSNP:746508104
589 589 c, t dbSNP:770241601
593 593 a, g dbSNP:200476948
605 605 c, t dbSNP:138895309
609 609 g, t dbSNP:372980079
623 623 c, g dbSNP:376206910
635 635 c, t dbSNP:774627072
636 636 a, c dbSNP:759704782
657 657 c, g dbSNP:767728271
658 658 a, g dbSNP:775474912
679 679 c, g dbSNP:758038664
686 686 c, g dbSNP:12014977
688 688 a, g dbSNP:751096381
699 699 a, g dbSNP:754497190
705 705 a, c dbSNP:780622232
709 709 a, g dbSNP:749796098
725 725 a, c dbSNP:771383792
733 733 a, g dbSNP:779268605
740 740 c, t dbSNP:370589511
749 749 c, t dbSNP:1043044
758 758 c, t dbSNP:775770634
782 782 a, g dbSNP:760754361
802 802 c, t dbSNP:768866249
805 805 a, g dbSNP:199838183
809 809 a, g dbSNP:761865238
810 810 g, t dbSNP:765245524
817 817 a, g dbSNP:750226910
820 820 c, g dbSNP:762802111
830 830 g, t dbSNP:766003948
831 831 c, g dbSNP:751237092
840 840 c, t dbSNP:754520173
854 854 c, t dbSNP:780707837
877 877 g, t dbSNP:752150960
878 878 c, t dbSNP:757807028
884 884 c, t dbSNP:779390979
891 891 c, t dbSNP:746278493
893 893 c, t dbSNP:772502489
895 895 a, g dbSNP:368156326
900 900 a, g dbSNP:780220478
924 924 c, g dbSNP:747287337
941 941 a, g dbSNP:111542041
946 946 c, g dbSNP:776901422
1009 1009 a, g dbSNP:372339836
1012 1012 c, t dbSNP:138036392
1017 1017 a, g dbSNP:755292405
1018 1018 c, t dbSNP:781562704
1022 1022 c, t dbSNP:749078330
1034 1034 a, g dbSNP:771039734
1037 1037 c, t dbSNP:748382114
1066 1066 a, c dbSNP:756360755
1067 1067 a, t dbSNP:777930279
1077 1077 c, t dbSNP:749305354
1079 1079 c, t dbSNP:149100572
1106 1106 c, t dbSNP:2071932
1112 1112 a, g dbSNP:745749102
1130 1130 a, g dbSNP:768020894
1131 1131 c, t dbSNP:191668283
1152 1152 c, g dbSNP:756408108
1170 1170 c, g dbSNP:184999100
1173 1173 a, g dbSNP:781549172
1188 1188 a, g dbSNP:139029259
1203 1203 c, t dbSNP:372304202
1217 1217 a, g dbSNP:762010356
1226 1226 c, g dbSNP:761467957
1250 1250 c, t dbSNP:769188952
1262 1262 c, t dbSNP:143026645
1278 1278 a, c, t dbSNP:147480000
1290 1290 g, t dbSNP:768115012
1316 1316 a, g dbSNP:138363965
1326 1326 c, t dbSNP:746882460
1337 1337 a, g dbSNP:141904298
1358 1358 c, t dbSNP:371362791
1363 1363 a, g dbSNP:758463316
1374 1374 a, c dbSNP:766390320
1418 1418 c, t dbSNP:753666567
1423 1423 a, g dbSNP:375724669
1429 1429 a, g dbSNP:778624859
1431 1431 c, t dbSNP:369099604
1433 1433 a, c dbSNP:757943408
1471 1471 a, g dbSNP:780966437
1493 1493 c, t dbSNP:150655488
1529 1529 c, t dbSNP:760744777
1539 1539 a, g dbSNP:764094559
1550 1550 c, t dbSNP:753752812
1552 1552 a, g dbSNP:765344449
1556 1556 a, g dbSNP:751416614
1568 1568 a, g dbSNP:11555927
1578 1578 g, t dbSNP:757951021
1587 1587 a, g dbSNP:779634966
1589 1589 a, g dbSNP:767508861
1591 1591 c, t dbSNP:756650378
1616 1616 c, t dbSNP:778245642
1630 1630 a, g dbSNP:749710147
1646 1646 c, t dbSNP:370758368
1652 1652 a, g dbSNP:779078378
1664 1664 c, t dbSNP:746132056
1670 1670 c, t dbSNP:149849605
1682 1682 c, t dbSNP:775755120
1694 1694 c, t dbSNP:747081998
1706 1706 c, t dbSNP:146407393
1715 1715 g, t dbSNP:752562003
1720 1720 a, g dbSNP:761593066
1724 1724 a, g dbSNP:139801905
1756 1756 g, t dbSNP:1804969
1774 1774 -, gtgcca dbSNP:752649471
1783 1783 c, g dbSNP:143128713
1792 1792 a, g dbSNP:762712208
1794 1794 -, aac dbSNP:758402011
1795 1795 a, g dbSNP:765982882
1796 1796 -, cagtgc dbSNP:777217330
1804 1804 a, g dbSNP:751132389
1808 1808 c, t dbSNP:754468012
1812 1812 -, gccagc dbSNP:745964344
1812 1812 a, g dbSNP:763080639
1816 1816 a, g dbSNP:766954953
1820 1820 c, t dbSNP:754347423
1829 1829 c, t dbSNP:1804968
1833 1833 a, g dbSNP:757663000
1850 1850 a, g dbSNP:11555934
1853 1853 c, t dbSNP:375313111
1854 1854 a, g dbSNP:376899197
1859 1859 g, t dbSNP:758716770
1878 1878 c, g, t dbSNP:200491129
1884 1884 c, t dbSNP:747171863
1893 1893 c, g dbSNP:768864626
1934 1934 c, t dbSNP:776815032
1967 1967 a, c dbSNP:1804967
2083 2083 a, t dbSNP:111765517
2086 2086 c, t dbSNP:377424961

Target ORF information:

RefSeq Version NM_014599
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D2 (MAGED2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014599

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGTCTGACA CAAGCGAGAG TGGTGCAGGT CTAACTCGCT TCCAGGCTGA AGCTTCAGAA 
AAGGACAGTA GCTCGATGAT GCAGACTCTG TTGACAGTGA CCCAGAATGT GGAGGTCCCA
GAGACACCGA AGGCCTCAAA GGCACTGGAG GTCTCAGAGG ATGTGAAGGT CTCAAAAGCC
TCTGGGGTCT CAAAGGCCAC AGAGGTCTCA AAGACCCCAG AGGCTCGGGA GGCACCTGCC
ACCCAGGCCT CATCTACTAC TCAGCTGACT GATACCCAGG TTCTGGCAGC TGAAAACAAG
AGTCTAGCAG CTGACACCAA GAAACAGAAT GCTGACCCGC AGGCTGTGAC AATGCCTGCC
ACTGAGACCA AAAAGGTCAG CCATGTGGCT GATACAAAGG TCAATACAAA GGCTCAGGAG
ACTGAGGCTG CACCCTCTCA GGCCCCAGCA GATGAACCTG AGCCTGAGAG TGCAGCTGCC
CAGTCTCAGG AGAATCAGGA TACTCGGCCC AAGGTCAAAG CCAAGAAAGC CCGAAAGGTG
AAGCATCTGG ATGGGGAAGA GGATGGCAGC AGTGATCAGA GTCAGGCTTC TGGAACCACA
GGTGGCCGAA GGGTCTCAAA GGCCCTAATG GCCTCAATGG CCCGCAGGGC TTCAAGGGGT
CCCATAGCCT TTTGGGCCCG CAGGGCATCA AGGACTCGGT TGGCTGCTTG GGCCCGGAGA
GCCTTGCTCT CCCTGAGATC ACCTAAAGCC CGTAGGGGCA AGGCTCGCCG TAGAGCTGCC
AAGCTCCAGT CATCCCAAGA GCCTGAAGCA CCACCACCTC GGGATGTGGC CCTTTTGCAA
GGGAGGGCAA ATGATTTGGT GAAGTACCTT TTGGCTAAAG ACCAGACGAA GATTCCCATC
AAGCGCTCGG ACATGCTGAA GGACATCATC AAAGAATACA CTGATGTGTA CCCCGAAATC
ATTGAACGAG CAGGCTATTC CTTGGAGAAG GTATTTGGGA TTCAATTGAA GGAAATTGAT
AAGAATGACC ACTTGTACAT TCTTCTCAGC ACCTTAGAGC CCACTGATGC AGGCATACTG
GGAACGACTA AGGACTCACC CAAGCTGGGT CTGCTCATGG TGCTTCTTAG CATCATCTTC
ATGAATGGAA ATCGGTCCAG TGAGGCTGTC ATCTGGGAGG TGCTGCGCAA GTTGGGGCTG
CGCCCTGGGA TACATCATTC ACTCTTTGGG GACGTGAAGA AGCTCATCAC TGATGAGTTT
GTGAAGCAGA AGTACCTGGA CTATGCCAGA GTCCCCAATA GCAATCCCCC TGAATATGAG
TTCTTCTGGG GCCTGCGCTC TTACTATGAG ACCAGCAAGA TGAAAGTCCT CAAGTTTGCC
TGCAAGGTAC AAAAGAAGGA TCCCAAGGAA TGGGCAGCTC AGTACCGAGA GGCGATGGAA
GCGGATTTGA AGGCTGCAGC TGAGGCTGCA GCTGAAGCCA AGGCTAGGGC CGAGATTAGA
GCTCGAATGG GCATTGGGCT CGGCTCGGAG AATGCTGCCG GGCCCTGCAA CTGGGACGAA
GCTGATATCG GACCCTGGGC CAAAGCCCGG ATCCAGGCGG GAGCAGAAGC TAAAGCCAAA
GCCCAAGAGA GTGGCAGTGC CAGCACTGGT GCCAGTACCA GTACCAATAA CAGTGCCAGT
GCCAGTGCCA GCACCAGTGG TGGCTTCAGT GCTGGTGCCA GCCTGACCGC CACTCTCACA
TTTGGGCTCT TCGCTGGCCT TGGTGGAGCT GGTGCCAGCA CCAGTGGCAG CTCTGGTGCC
TGTGGTTTCT CCTACAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.