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YWHAQ cDNA ORF clone, Homo sapiens (human)

Gene Symbol YWHAQ
Entrez Gene ID 10971
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
Synonyms 14-3-3, 1C5, HS1
General protein information
Preferred Names
14-3-3 protein theta
Names
14-3-3 protein theta
14-3-3 protein tau
14-3-3 protein T-cell
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p25.1

Summary This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609009

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_006826 NP_006817 14-3-3 protein theta

KEGG
hsa04110 Cell cycle
hsa05130 Pathogenic Escherichia coli infection
hsa04114 Oocyte meiosis
hsa05203 Viral carcinogenesis
hsa04151 PI3K-Akt signaling pathway
hsa05161 Hepatitis B
hsa05169 Epstein-Barr virus infection
hsa04390 Hippo signaling pathway
Reactome
R-HSA-162582 Signal Transduction
R-HSA-194315 Signaling by Rho GTPases
R-HSA-195258 RHO GTPase Effectors
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-74160 Gene Expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-1640170 Cell Cycle
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
R-HSA-69481 G2/M Checkpoints
R-HSA-69473 G2/M DNA damage checkpoint
R-HSA-199991 Membrane Trafficking
R-HSA-5653656 Vesicle-mediated transport
R-HSA-1445148 Translocation of GLUT4 to the plasma membrane
R-HSA-109581 Apoptosis
R-HSA-114452 Activation of BH3-only proteins
R-HSA-5357801 Programmed Cell Death
R-HSA-111447 Activation of BAD and translocation to mitochondria
R-HSA-109606 Intrinsic Pathway for Apoptosis
Pathway Interaction Database
wnt_beta_catenin_pathway Regulation of Wnt-mediated beta catenin signaling and target gene transcription
nfat_3pathway Role of Calcineurin-dependent NFAT signaling in lymphocytes
pi3kplctrkpathway Trk receptor signaling mediated by PI3K and PLC-gamma
a6b1_a6b4_integrin_pathway a6b1 and a6b4 Integrin signaling
insulin_glucose_pathway Insulin-mediated glucose transport
pi3kciaktpathway Class I PI3K signaling events mediated by Akt
mtor_4pathway mTOR signaling pathway
foxopathway FoxO family signaling
lkb1_pathway LKB1 signaling events
erbb1_downstream_pathway ErbB1 downstream signaling
p38_mk2pathway p38 signaling mediated by MAPKAP kinases
WikiPathways
WP289 Myometrial Relaxation and Contraction Pathways
WP179 Cell cycle
WP244 Alpha6-Beta4 Integrin Signaling Pathway
WP231 TNF-alpha/NF-kB Signaling Pathway
WP706 SIDS Susceptibility Pathways
WP286 IL-3 Signaling Pathway
WP536 Calcium Regulation in the Cardiac Cell
WP2272 Pathogenic Escherichia coli infection


Homo sapiens (human) YWHAQ NP_006817.1
Pan troglodytes (chimpanzee) YWHAQ XP_003949798.1
Macaca mulatta (Rhesus monkey) YWHAQ XP_001097635.1
Canis lupus familiaris (dog) YWHAQ XP_532871.1
Bos taurus (cattle) YWHAQ NP_001071595.1
Mus musculus (house mouse) Ywhaq NP_035869.1
Mus musculus (house mouse) LOC102634437 XP_006538470.1
Rattus norvegicus (Norway rat) Ywhaq NP_037185.1
Gallus gallus (chicken) YWHAQ NP_001006415.1
Danio rerio (zebrafish) ywhaqb NP_958892.1
Xenopus (Silurana) tropicalis (western clawed frog) ywhaq NP_001025570.1

Component

ID Name Evidence
GO:0005625 soluble fraction IEA
GO:0005634 nucleus IDA
GO:0005730 nucleolus IDA
GO:0005737 cytoplasm IDA
GO:0005813 centrosome IDA
GO:0043234 protein complex IEA

Function

ID Name Evidence
GO:0005515 protein binding IPI
GO:0019904 protein domain specific binding IEA
GO:0047485 protein N-terminus binding IPI

Process

ID Name Evidence
GO:0006605 protein targeting IEA
GO:0007264 small GTPase mediated signal transduction IEA
GO:0045892 negative regulation of transcription, DNA-dependent IDA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following YWHAQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YWHAQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16909
NM_006826 Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta (YWHAQ), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu16909
Accession Version NM_006826.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 738bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 14-3-3 protein theta
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY044745.1, BC093019.1, BC050601.1 and AA729400.1. On Jun 21, 2013 this sequence version replaced gi:21464103. Summary: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056867.1, X57347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGAAGA CTGAGCTGAT CCAGAAGGCC AAGCTGGCCG AGCAGGCCGA GCGCTACGAC 
GACATGGCCA CCTGCATGAA GGCAGTGACC GAGCAGGGCG CCGAGCTGTC CAACGAGGAG
CGCAACCTGC TCTCCGTGGC CTACAAGAAC GTGGTCGGGG GCCGCAGGTC CGCCTGGAGG
GTCATCTCTA GCATCGAGCA GAAGACCGAC ACCTCCGACA AGAAGTTGCA GCTGATTAAG
GACTATCGGG AGAAAGTGGA GTCCGAGCTG AGATCCATCT GCACCACGGT GCTGGAATTG
TTGGATAAAT ATTTAATAGC CAATGCAACT AATCCAGAGA GTAAGGTCTT CTATCTGAAA
ATGAAGGGTG ATTACTTCCG GTACCTTGCT GAAGTTGCGT GTGGTGATGA TCGAAAACAA
ACGATAGATA ATTCCCAAGG AGCTTACCAA GAGGCATTTG ATATAAGCAA GAAAGAGATG
CAACCCACAC ACCCAATCCG CCTGGGGCTT GCTCTTAACT TTTCTGTATT TTACTATGAG
ATTCTTAATA ACCCAGAGCT TGCCTGCACG CTGGCTAAAA CGGCTTTTGA TGAGGCCATT
GCTGAACTTG ATACACTGAA TGAAGACTCA TACAAAGACA GCACCCTCAT CATGCAGTTG
CTTAGAGACA ACCTAACACT TTGGACATCA GACAGTGCAG GAGAAGAATG TGATGCGGCA
GAAGGGGCTG AAAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_006817.1
CDS198..935
Misc Feature(1)111..113(+)
Misc Feature(2)198..899(+)
Misc Feature(3)198..200(+)
Misc Feature(4)204..206(+)
Misc Feature(5)219..464(+)
Misc Feature(6)270..272(+)
Misc Feature(7)342..881(+)
Misc Feature(8)342..344(+)
Misc Feature(9)363..365(+)
Misc Feature(10)399..401(+)
Misc Feature(11)540..542(+)
Misc Feature(12)576..578(+)
Misc Feature(13)885..887(+)
Misc Feature(14)891..893(+)
Misc Feature(15)891..893(+)
Misc Feature(16)891..893(+)
Exon (1)1..115
Gene:YWHAQ
Gene Synonym:
Exon (2)116..491
Gene:YWHAQ
Gene Synonym:
Exon (3)492..615
Gene:YWHAQ
Gene Synonym:
Exon (4)616..779
Gene:YWHAQ
Gene Synonym:
Exon (5)780..875
Gene:YWHAQ
Gene Synonym:
Exon (6)876..2254
Gene:YWHAQ
Gene Synonym:
Translation
Position Chain Variation Link
16 16 c, t dbSNP:572934831
21 21 c, t dbSNP:62119435
43 43 a, g dbSNP:553083018
55 55 a, c dbSNP:762935608
63 63 c, g dbSNP:539970885
65 65 a, g dbSNP:577222475
91 91 c, g dbSNP:557541708
97 97 c, g dbSNP:537609418
100 100 c, t dbSNP:144636684
130 130 a, c dbSNP:11544463
134 134 c, t dbSNP:561528893
138 138 a, g dbSNP:541763260
149 149 a, c dbSNP:764174434
153 153 c, t dbSNP:755053877
155 155 c, g dbSNP:751552379
158 158 c, t dbSNP:766499891
159 159 c, g dbSNP:763024118
161 161 c, t dbSNP:773424346
163 163 c, t dbSNP:765579544
165 165 a, g dbSNP:762217816
166 166 c, g, t dbSNP:1130874
167 167 c, t dbSNP:375411374
169 169 c, t dbSNP:774805872
169 169 -, t dbSNP:776483508
170 170 c, t dbSNP:771615816
171 171 -, tcgccctcc dbSNP:760535815
171 171 c, g dbSNP:745504240
172 172 -, acccgg dbSNP:774663201
173 173 c, t dbSNP:778766840
175 175 a, c dbSNP:559702166
176 176 c, g, t dbSNP:777741592
178 178 g, t dbSNP:755954786
180 180 a, c dbSNP:11544466
180 180 c, g dbSNP:752690192
182 182 c, t dbSNP:779927922
184 184 -, gccccg dbSNP:773471715
185 185 c, t dbSNP:758568866
186 186 a, c dbSNP:11544462
187 187 a, c dbSNP:11544464
188 188 c, g dbSNP:545839277
191 191 g, t dbSNP:765485923
194 194 c, g, t dbSNP:754157288
196 196 c, t dbSNP:764536655
212 212 a, g dbSNP:761020810
226 226 c, g dbSNP:776134213
236 236 c, t dbSNP:368668324
255 255 a, c, g dbSNP:774114759
257 257 c, g dbSNP:770667690
260 260 c, t dbSNP:748970639
269 269 a, c dbSNP:777649657
277 277 a, g dbSNP:769631927
281 281 a, g dbSNP:748029699
282 282 a, g dbSNP:141887759
288 288 c, g dbSNP:758380692
294 294 a, g dbSNP:750464603
295 295 g, t dbSNP:778991719
297 297 c, g dbSNP:757416206
299 299 c, t dbSNP:754065297
308 308 c, t dbSNP:377250330
310 310 a, c dbSNP:756561669
314 314 a, g dbSNP:372978538
320 320 c, t dbSNP:201957113
322 322 a, g dbSNP:760149820
324 324 c, t dbSNP:773949858
332 332 c, g, t dbSNP:762624973
335 335 a, c, g dbSNP:769460753
338 338 c, t dbSNP:748020959
350 350 a, g dbSNP:539717165
369 369 g, t dbSNP:776325430
383 383 c, t dbSNP:768534041
387 387 a, g dbSNP:746966183
397 397 a, g dbSNP:778901859
404 404 c, g dbSNP:757395602
408 408 a, g dbSNP:749499495
414 414 a, g dbSNP:11544465
416 416 c, t dbSNP:202138500
418 418 a, g dbSNP:756400871
425 425 a, g dbSNP:753064140
436 436 a, g dbSNP:199958721
437 437 a, g dbSNP:755484324
442 442 a, g dbSNP:752112912
451 451 a, g dbSNP:774239040
468 468 a, c dbSNP:372545955
473 473 c, t dbSNP:762606877
474 474 a, t dbSNP:369852702
476 476 c, t dbSNP:764907087
481 481 a, c dbSNP:761396781
485 485 c, g dbSNP:776433787
498 498 c, t dbSNP:112226455
518 518 c, g dbSNP:760512737
520 520 a, g dbSNP:775375833
525 525 a, g dbSNP:185211853
533 533 a, g dbSNP:759412655
596 596 a, g dbSNP:773284973
615 615 c, t dbSNP:769768341
619 619 c, t dbSNP:767312364
621 621 a, g dbSNP:759450776
626 626 c, t dbSNP:751249633
636 636 c, g dbSNP:774221614
639 639 a, g dbSNP:769900540
647 647 a, g dbSNP:761844291
651 651 g, t dbSNP:371354151
661 661 c, t dbSNP:768956586
668 668 a, g dbSNP:201487177
669 669 a, g dbSNP:199515928
671 671 a, g dbSNP:144863616
680 680 a, c, g dbSNP:780502121
681 681 c, t dbSNP:772387887
686 686 a, c dbSNP:746377347
693 693 a, g dbSNP:200884745
697 697 a, g, t dbSNP:367832498
699 699 c, t dbSNP:777288889
704 704 a, g dbSNP:755703871
722 722 c, t dbSNP:752349662
749 749 c, t dbSNP:767340019
752 752 a, g dbSNP:754783750
755 755 a, g dbSNP:751488523
766 766 c, t dbSNP:150523147
767 767 a, g dbSNP:762976369
775 775 a, g dbSNP:776796788
781 781 c, t dbSNP:377175915
795 795 a, g dbSNP:774523757
811 811 a, c, t dbSNP:749789995
829 829 a, t dbSNP:773765562
841 841 c, t dbSNP:769267624
867 867 a, g dbSNP:747693562
892 892 c, g dbSNP:762328022
893 893 c, t dbSNP:775943040
896 896 a, t dbSNP:768281092
898 898 -, gag dbSNP:752577017
906 906 g, t dbSNP:746631800
910 910 a, t dbSNP:779806817
913 913 c, g, t dbSNP:200376025
914 914 a, g dbSNP:373178591
921 921 a, g dbSNP:757206430
925 925 c, t dbSNP:753821484
932 932 c, t dbSNP:148399453
935 935 a, g dbSNP:199737672
938 938 a, c dbSNP:781125712
940 940 a, g dbSNP:755141288
948 948 c, t dbSNP:780100582
953 953 c, t dbSNP:191830707
954 954 c, t dbSNP:145097891
955 955 c, g, t dbSNP:41264177
956 956 g, t dbSNP:375784718
958 958 -, ctt dbSNP:766682675
961 961 g, t dbSNP:369470995
972 972 a, c dbSNP:762224503
981 981 c, t dbSNP:777255377
982 982 a, t dbSNP:376453415
1027 1027 c, t dbSNP:16822436
1045 1045 a, g dbSNP:750627405
1079 1079 c, t dbSNP:573366066
1086 1086 a, c dbSNP:16844798
1104 1104 g, t dbSNP:76429341
1134 1134 c, t dbSNP:374886445
1196 1196 c, t dbSNP:112750534
1213 1213 c, g dbSNP:574721044
1227 1227 a, t dbSNP:187340849
1267 1267 g, t dbSNP:541193418
1285 1285 a, g dbSNP:773564569
1290 1290 c, g dbSNP:371569671
1296 1296 -, t dbSNP:763943503
1349 1349 -, a dbSNP:759457637
1500 1500 a, g dbSNP:184509644
1506 1506 c, g dbSNP:192318359
1511 1511 a, g dbSNP:368172501
1536 1536 a, t dbSNP:553450887
1548 1548 -, t dbSNP:772370875
1583 1583 g, t dbSNP:11544459
1591 1591 a, c dbSNP:576756744
1600 1600 a, c dbSNP:189104400
1629 1629 a, g dbSNP:542996766
1685 1685 a, g dbSNP:574288268
1686 1686 c, t dbSNP:182444865
1719 1719 a, t dbSNP:762031314
1731 1731 -, taaat dbSNP:60152522
1735 1735 -, ttaaa dbSNP:147701876
1751 1751 c, t dbSNP:748392867
1765 1765 c, t dbSNP:537163310
1777 1777 a, g dbSNP:774661836
1808 1808 c, t dbSNP:191107303
1839 1839 g, t dbSNP:568125543
1842 1842 a, g dbSNP:548411732
1847 1847 c, t dbSNP:534763265
1874 1874 a, t dbSNP:6758214
1954 1954 -, a dbSNP:540708954
1998 1998 a, g dbSNP:552177354
2053 2053 a, g dbSNP:532571933
2068 2068 a, g dbSNP:563513298
2078 2078 a, g dbSNP:187680337
2083 2083 a, g dbSNP:7594
2085 2085 -, attgtga dbSNP:757592569
2143 2143 -, a dbSNP:753719464
2205 2205 a, g dbSNP:561007341
2233 2233 a, g dbSNP:541231706

Target ORF information:

RefSeq Version NM_006826
Organism Homo sapiens (human)
Definition Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta (YWHAQ), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006826

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGAAGA CTGAGCTGAT CCAGAAGGCC AAGCTGGCCG AGCAGGCCGA GCGCTACGAC 
GACATGGCCA CCTGCATGAA GGCAGTGACC GAGCAGGGCG CCGAGCTGTC CAACGAGGAG
CGCAACCTGC TCTCCGTGGC CTACAAGAAC GTGGTCGGGG GCCGCAGGTC CGCCTGGAGG
GTCATCTCTA GCATCGAGCA GAAGACCGAC ACCTCCGACA AGAAGTTGCA GCTGATTAAG
GACTATCGGG AGAAAGTGGA GTCCGAGCTG AGATCCATCT GCACCACGGT GCTGGAATTG
TTGGATAAAT ATTTAATAGC CAATGCAACT AATCCAGAGA GTAAGGTCTT CTATCTGAAA
ATGAAGGGTG ATTACTTCCG GTACCTTGCT GAAGTTGCGT GTGGTGATGA TCGAAAACAA
ACGATAGATA ATTCCCAAGG AGCTTACCAA GAGGCATTTG ATATAAGCAA GAAAGAGATG
CAACCCACAC ACCCAATCCG CCTGGGGCTT GCTCTTAACT TTTCTGTATT TTACTATGAG
ATTCTTAATA ACCCAGAGCT TGCCTGCACG CTGGCTAAAA CGGCTTTTGA TGAGGCCATT
GCTGAACTTG ATACACTGAA TGAAGACTCA TACAAAGACA GCACCCTCAT CATGCAGTTG
CTTAGAGACA ACCTAACACT TTGGACATCA GACAGTGCAG GAGAAGAATG TGATGCGGCA
GAAGGGGCTG AAAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of a novel ATPase activity in 14-3-3 proteins--evidence from enzyme kinetics, structure guided modeling and mutagenesis studies
FEBS Lett. 588 (1), 71-78 (2014)
Ramteke MP, Shelke P, Ramamoorthy V, Somavarapu AK, Gautam AK, Nanaware PP, Karanam S, Mukhopadhyay S and Venkatraman P.


book

14-3-3 proteins modulate the ETS transcription factor ETV1 in prostate cancer
Cancer Res. 73 (16), 5110-5119 (2013)
Oh S, Shin S, Lightfoot SA and Janknecht R.


book

Negative regulation of the RalGAP complex by 14-3-3
J. Biol. Chem. 288 (13), 9272-9283 (2013)
Leto D, Uhm M, Williams A, Chen XW and Saltiel AR.


book

Oligomeric structure of 14-3-3 protein: what do we know about monomers?
FEBS Lett. 586 (24), 4249-4256 (2012)
Sluchanko NN and Gusev NB.


book

Cerebrospinal fluid biomarker supported diagnosis of Creutzfeldt-Jakob disease and rapid dementias: a longitudinal multicentre study over 10 years
Brain 135 (PT 10), 3051-3061 (2012)
Stoeck K, Sanchez-Juan P, Gawinecka J, Green A, Ladogana A, Pocchiari M, Sanchez-Valle R, Mitrova E, Sklaviadis T, Kulczycki J, Slivarichova D, Saiz A, Calero M, Knight R, Aguzzi A, Laplanche JL, Peoc'h K, Schelzke G, Karch A, van Duijn CM and Zerr I.


book

Activation-modulated association of 14-3-3 proteins with Cbl in T cells
J. Biol. Chem. 271 (24), 14591-14595 (1996)
Liu YC, Elly C, Yoshida H, Bonnefoy-Berard N and Altman A.


book

Inhibition of phosphatidylinositol 3-kinase activity by association with 14-3-3 proteins in T cells
Proc. Natl. Acad. Sci. U.S.A. 92 (22), 10142-10146 (1995)
Bonnefoy-Berard N, Liu YC, von Willebrand M, Sung A, Elly C, Mustelin T, Yoshida H, Ishizaka K and Altman A.


book

Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways
Nature 376 (6536), 188-191 (1995)
Xiao B, Smerdon SJ, Jones DH, Dodson GG, Soneji Y, Aitken A and Gamblin SJ.


book

Molecular cloning and expression of the transformation sensitive epithelial marker stratifin. A member of a protein family that has been involved in the protein kinase C signalling pathway
J. Mol. Biol. 231 (4), 982-998 (1993)
Leffers H, Madsen P, Rasmussen HH, Honore B, Andersen AH, Walbum E, Vandekerckhove J and Celis JE.


book

Primary structure of a human protein kinase regulator protein
Biochim. Biophys. Acta 1088 (3), 425-428 (1991)
Nielsen PJ.


 
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