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YWHAQ cDNA ORF clone, Homo sapiens (human)

Gene Symbol YWHAQ
Entrez Gene ID 10971
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
Synonyms 14-3-3, 1C5, HS1
General protein information
Preferred Names
14-3-3 protein theta
Names
14-3-3 protein theta
14-3-3 protein tau
14-3-3 protein T-cell
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p25.1

Summary This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

609009


mRNA and Protein(s)

mRNA Protein Name
NM_006826 NP_006817 14-3-3 protein theta



Homo sapiens (human) YWHAQ NP_006817.1
Pan troglodytes (chimpanzee) YWHAQ XP_003949798.1
Macaca mulatta (Rhesus monkey) YWHAQ XP_001097635.1
Canis lupus familiaris (dog) YWHAQ XP_532871.1
Bos taurus (cattle) YWHAQ NP_001071595.1
Mus musculus (house mouse) Ywhaq NP_035869.1
Mus musculus (house mouse) LOC102634437 XP_006538470.1
Rattus norvegicus (Norway rat) Ywhaq NP_037185.1
Gallus gallus (chicken) YWHAQ NP_001006415.1
Danio rerio (zebrafish) ywhaqb NP_958892.1
Xenopus (Silurana) tropicalis (western clawed frog) ywhaq NP_001025570.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following YWHAQ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YWHAQ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16909
NM_006826 Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta (YWHAQ), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16909
Clone ID Related Accession (Same CDS sequence) NM_006826
Accession Version NM_006826.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 738bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 14-3-3 protein theta
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY044745.1, BC093019.1, BC050601.1 and AA729400.1. On Jun 21, 2013 this sequence version replaced gi:21464103. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056867.1, X57347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGAAGA CTGAGCTGAT CCAGAAGGCC AAGCTGGCCG AGCAGGCCGA GCGCTACGAC 
GACATGGCCA CCTGCATGAA GGCAGTGACC GAGCAGGGCG CCGAGCTGTC CAACGAGGAG
CGCAACCTGC TCTCCGTGGC CTACAAGAAC GTGGTCGGGG GCCGCAGGTC CGCCTGGAGG
GTCATCTCTA GCATCGAGCA GAAGACCGAC ACCTCCGACA AGAAGTTGCA GCTGATTAAG
GACTATCGGG AGAAAGTGGA GTCCGAGCTG AGATCCATCT GCACCACGGT GCTGGAATTG
TTGGATAAAT ATTTAATAGC CAATGCAACT AATCCAGAGA GTAAGGTCTT CTATCTGAAA
ATGAAGGGTG ATTACTTCCG GTACCTTGCT GAAGTTGCGT GTGGTGATGA TCGAAAACAA
ACGATAGATA ATTCCCAAGG AGCTTACCAA GAGGCATTTG ATATAAGCAA GAAAGAGATG
CAACCCACAC ACCCAATCCG CCTGGGGCTT GCTCTTAACT TTTCTGTATT TTACTATGAG
ATTCTTAATA ACCCAGAGCT TGCCTGCACG CTGGCTAAAA CGGCTTTTGA TGAGGCCATT
GCTGAACTTG ATACACTGAA TGAAGACTCA TACAAAGACA GCACCCTCAT CATGCAGTTG
CTTAGAGACA ACCTAACACT TTGGACATCA GACAGTGCAG GAGAAGAATG TGATGCGGCA
GAAGGGGCTG AAAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006817.1
CDS198..935
Misc Feature(1)111..113(+)
Misc Feature(2)198..899(+)
Misc Feature(3)198..200(+)
Misc Feature(4)204..206(+)
Misc Feature(5)219..464(+)
Misc Feature(6)270..272(+)
Misc Feature(7)342..881(+)
Misc Feature(8)342..344(+)
Misc Feature(9)363..365(+)
Misc Feature(10)399..401(+)
Misc Feature(11)540..542(+)
Misc Feature(12)576..578(+)
Misc Feature(13)885..887(+)
Misc Feature(14)891..893(+)
Misc Feature(15)891..893(+)
Misc Feature(16)891..893(+)
Exon (1)1..115
Gene:YWHAQ
Gene Synonym:
Exon (2)116..491
Gene:YWHAQ
Gene Synonym:
Exon (3)492..615
Gene:YWHAQ
Gene Synonym:
Exon (4)616..779
Gene:YWHAQ
Gene Synonym:
Exon (5)780..875
Gene:YWHAQ
Gene Synonym:
Exon (6)876..2254
Gene:YWHAQ
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006826
Organism Homo sapiens (human)
Definition Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta (YWHAQ), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006826

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGAGAAGA CTGAGCTGAT CCAGAAGGCC AAGCTGGCCG AGCAGGCCGA GCGCTACGAC 
GACATGGCCA CCTGCATGAA GGCAGTGACC GAGCAGGGCG CCGAGCTGTC CAACGAGGAG
CGCAACCTGC TCTCCGTGGC CTACAAGAAC GTGGTCGGGG GCCGCAGGTC CGCCTGGAGG
GTCATCTCTA GCATCGAGCA GAAGACCGAC ACCTCCGACA AGAAGTTGCA GCTGATTAAG
GACTATCGGG AGAAAGTGGA GTCCGAGCTG AGATCCATCT GCACCACGGT GCTGGAATTG
TTGGATAAAT ATTTAATAGC CAATGCAACT AATCCAGAGA GTAAGGTCTT CTATCTGAAA
ATGAAGGGTG ATTACTTCCG GTACCTTGCT GAAGTTGCGT GTGGTGATGA TCGAAAACAA
ACGATAGATA ATTCCCAAGG AGCTTACCAA GAGGCATTTG ATATAAGCAA GAAAGAGATG
CAACCCACAC ACCCAATCCG CCTGGGGCTT GCTCTTAACT TTTCTGTATT TTACTATGAG
ATTCTTAATA ACCCAGAGCT TGCCTGCACG CTGGCTAAAA CGGCTTTTGA TGAGGCCATT
GCTGAACTTG ATACACTGAA TGAAGACTCA TACAAAGACA GCACCCTCAT CATGCAGTTG
CTTAGAGACA ACCTAACACT TTGGACATCA GACAGTGCAG GAGAAGAATG TGATGCGGCA
GAAGGGGCTG AAAACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of a novel ATPase activity in 14-3-3 proteins--evidence from enzyme kinetics, structure guided modeling and mutagenesis studies
FEBS Lett. 588 (1), 71-78 (2014)
Ramteke MP, Shelke P, Ramamoorthy V, Somavarapu AK, Gautam AK, Nanaware PP, Karanam S, Mukhopadhyay S and Venkatraman P.


book

14-3-3 proteins modulate the ETS transcription factor ETV1 in prostate cancer
Cancer Res. 73 (16), 5110-5119 (2013)
Oh S, Shin S, Lightfoot SA and Janknecht R.


book

Negative regulation of the RalGAP complex by 14-3-3
J. Biol. Chem. 288 (13), 9272-9283 (2013)
Leto D, Uhm M, Williams A, Chen XW and Saltiel AR.


book

Oligomeric structure of 14-3-3 protein: what do we know about monomers?
FEBS Lett. 586 (24), 4249-4256 (2012)
Sluchanko NN and Gusev NB.


book

Cerebrospinal fluid biomarker supported diagnosis of Creutzfeldt-Jakob disease and rapid dementias: a longitudinal multicentre study over 10 years
Brain 135 (PT 10), 3051-3061 (2012)
Stoeck K, Sanchez-Juan P, Gawinecka J, Green A, Ladogana A, Pocchiari M, Sanchez-Valle R, Mitrova E, Sklaviadis T, Kulczycki J, Slivarichova D, Saiz A, Calero M, Knight R, Aguzzi A, Laplanche JL, Peoc'h K, Schelzke G, Karch A, van Duijn CM and Zerr I.


book

Activation-modulated association of 14-3-3 proteins with Cbl in T cells
J. Biol. Chem. 271 (24), 14591-14595 (1996)
Liu YC, Elly C, Yoshida H, Bonnefoy-Berard N and Altman A.


book

Inhibition of phosphatidylinositol 3-kinase activity by association with 14-3-3 proteins in T cells
Proc. Natl. Acad. Sci. U.S.A. 92 (22), 10142-10146 (1995)
Bonnefoy-Berard N, Liu YC, von Willebrand M, Sung A, Elly C, Mustelin T, Yoshida H, Ishizaka K and Altman A.


book

Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways
Nature 376 (6536), 188-191 (1995)
Xiao B, Smerdon SJ, Jones DH, Dodson GG, Soneji Y, Aitken A and Gamblin SJ.


book

Molecular cloning and expression of the transformation sensitive epithelial marker stratifin. A member of a protein family that has been involved in the protein kinase C signalling pathway
J. Mol. Biol. 231 (4), 982-998 (1993)
Leffers H, Madsen P, Rasmussen HH, Honore B, Andersen AH, Walbum E, Vandekerckhove J and Celis JE.


book

Primary structure of a human protein kinase regulator protein
Biochim. Biophys. Acta 1088 (3), 425-428 (1991)
Nielsen PJ.


 
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