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CHAT cDNA ORF clone, Homo sapiens (human)

Gene Symbol CHAT
Entrez Gene ID 1103
Full Name choline O-acetyltransferase
Synonyms CHOACTASE, CMS1A, CMS1A2, CMS6
General protein information
Preferred Names
choline O-acetyltransferase
Names
choline O-acetyltransferase
choline acetylase
acetyl CoA:choline O-acetyltransferase
NP_001136401.1
NP_001136405.1
NP_001136406.1
NP_065574.3
NP_066264.3
NP_066265.3
NP_066266.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q11.2

Summary This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]. lac of sum
Disorder MIM:

118490

Disorder Html: Myasthenic syndrome, congenital, associated with episodic apnea,

mRNA and Protein(s)

mRNA Protein Name
NM_020984 NP_066264 choline O-acetyltransferase isoform 1
NM_001142934 NP_001136406 choline O-acetyltransferase isoform 1
NM_020986 NP_066266 choline O-acetyltransferase isoform 1
NM_001142929 NP_001136401 choline O-acetyltransferase isoform 1
NM_020985 NP_066265 choline O-acetyltransferase isoform 1
NM_020549 NP_065574 choline O-acetyltransferase isoform 2
NM_001142933 NP_001136405 choline O-acetyltransferase isoform 3



Homo sapiens (human) CHAT NP_001136405.1
Pan troglodytes (chimpanzee) CHAT XP_003339420.1
Macaca mulatta (Rhesus monkey) CHAT XP_002805679.1
Canis lupus familiaris (dog) CHAT XP_005637542.1
Bos taurus (cattle) CHAT XP_002698999.2
Mus musculus (house mouse) Chat NP_034021.1
Rattus norvegicus (Norway rat) Chat NP_001164064.1
Gallus gallus (chicken) CHAT NP_989941.1
Danio rerio (zebrafish) chat NP_001124191.1
Drosophila melanogaster (fruit fly) Cha NP_477004.5
Caenorhabditis elegans cha-1 NP_001023603.1
Xenopus (Silurana) tropicalis (western clawed frog) chat XP_002933959.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16386 NM_020984 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant R, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu16386 NM_001142934 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu16386 NM_020986 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu16386 NM_001142929 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu16386 NM_020985 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu19162 NM_020549 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $531.30
$759.00
OHu16430 NM_001142933 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $468.30
$669.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16386
Clone ID Related Accession (Same CDS sequence) NM_001142929 , NM_001142934 , NM_020984 , NM_020985 , NM_020986
Accession Version NM_020984.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF305909.1 and AC073366.4. This sequence is a reference standard in the RefSeqGene project. On May 21, 2008 this sequence version replaced gi:119433672. Transcript Variant: This variant (R) contains alternate 5' exon R (PMID 7616604) and encodes isoform 1. Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305909.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066264.3
CDS174..2066
Misc Feature(1)63..65(+)
Misc Feature(2)210..1979(+)
Exon (1)1..105
Gene:CHAT
Gene Synonym:
Exon (2)106..206
Gene:CHAT
Gene Synonym:
Exon (3)207..398
Gene:CHAT
Gene Synonym:
Exon (4)399..517
Gene:CHAT
Gene Synonym:
Exon (5)518..571
Gene:CHAT
Gene Synonym:
Exon (6)572..752
Gene:CHAT
Gene Synonym:
Exon (7)753..930
Gene:CHAT
Gene Synonym:
Exon (8)931..1100
Gene:CHAT
Gene Synonym:
Exon (9)1101..1201
Gene:CHAT
Gene Synonym:
Exon (10)1202..1330
Gene:CHAT
Gene Synonym:
Exon (11)1331..1453
Gene:CHAT
Gene Synonym:
Exon (12)1454..1595
Gene:CHAT
Gene Synonym:
Exon (13)1596..1658
Gene:CHAT
Gene Synonym:
Exon (14)1659..1796
Gene:CHAT
Gene Synonym:
Exon (15)1797..2124
Gene:CHAT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020984
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant R, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020984

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16386
Clone ID Related Accession (Same CDS sequence) NM_001142929 , NM_001142934 , NM_020984 , NM_020985 , NM_020986
Accession Version NM_001142934.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF305908.2 and AC073366.4. Transcript Variant: This variant (S) contains alternate 5' exons M and S in the 5' coding region but uses a downstream start codon and encodes isoform 1 (PMID 11172068). Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305908.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1337937) ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001136406.1
CDS578..2470
Misc Feature(1)440..442(+)
Misc Feature(2)614..2383(+)
Exon (1)1..439
Gene:CHAT
Gene Synonym:
Exon (2)440..509
Gene:CHAT
Gene Synonym:
Exon (3)510..610
Gene:CHAT
Gene Synonym:
Exon (4)611..802
Gene:CHAT
Gene Synonym:
Exon (5)803..921
Gene:CHAT
Gene Synonym:
Exon (6)922..975
Gene:CHAT
Gene Synonym:
Exon (7)976..1156
Gene:CHAT
Gene Synonym:
Exon (8)1157..1334
Gene:CHAT
Gene Synonym:
Exon (9)1335..1504
Gene:CHAT
Gene Synonym:
Exon (10)1505..1605
Gene:CHAT
Gene Synonym:
Exon (11)1606..1734
Gene:CHAT
Gene Synonym:
Exon (12)1735..1857
Gene:CHAT
Gene Synonym:
Exon (13)1858..1999
Gene:CHAT
Gene Synonym:
Exon (14)2000..2062
Gene:CHAT
Gene Synonym:
Exon (15)2063..2200
Gene:CHAT
Gene Synonym:
Exon (16)2201..2528
Gene:CHAT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001142934
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001142934

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16386
Clone ID Related Accession (Same CDS sequence) NM_001142929 , NM_001142934 , NM_020984 , NM_020985 , NM_020986
Accession Version NM_020986.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D82340.1, D82341.1, AC073366.4, BC130615.1 and AF305907.1. On May 21, 2008 this sequence version replaced gi:119433670. Transcript Variant: This variant (N2) contains alternate 5' exon N and encodes isoform 1 (PMID 9073174). Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF305909.1, BC130615.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066266.3
CDS164..2056
Misc Feature(1)11..13(+)
Misc Feature(2)200..1969(+)
Exon (1)1..95
Gene:CHAT
Gene Synonym:
Exon (2)96..196
Gene:CHAT
Gene Synonym:
Exon (3)197..388
Gene:CHAT
Gene Synonym:
Exon (4)389..507
Gene:CHAT
Gene Synonym:
Exon (5)508..561
Gene:CHAT
Gene Synonym:
Exon (6)562..742
Gene:CHAT
Gene Synonym:
Exon (7)743..920
Gene:CHAT
Gene Synonym:
Exon (8)921..1090
Gene:CHAT
Gene Synonym:
Exon (9)1091..1191
Gene:CHAT
Gene Synonym:
Exon (10)1192..1320
Gene:CHAT
Gene Synonym:
Exon (11)1321..1443
Gene:CHAT
Gene Synonym:
Exon (12)1444..1585
Gene:CHAT
Gene Synonym:
Exon (13)1586..1648
Gene:CHAT
Gene Synonym:
Exon (14)1649..1786
Gene:CHAT
Gene Synonym:
Exon (15)1787..2114
Gene:CHAT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020986
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020986

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16386
Clone ID Related Accession (Same CDS sequence) NM_001142929 , NM_001142934 , NM_020984 , NM_020985 , NM_020986
Accession Version NM_001142929.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073366.4 and AF305907.1. Transcript Variant: This variant (M) contains alternate 5' exon M (PMID 7616604) but uses a downstream start codon and encodes isoform 1. Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305907.1, S56138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1337937) ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001136401.1
CDS508..2400
Misc Feature(1)7..9(+)
Misc Feature(2)544..2313(+)
Exon (1)1..439
Gene:CHAT
Gene Synonym:
Exon (2)440..540
Gene:CHAT
Gene Synonym:
Exon (3)541..732
Gene:CHAT
Gene Synonym:
Exon (4)733..851
Gene:CHAT
Gene Synonym:
Exon (5)852..905
Gene:CHAT
Gene Synonym:
Exon (6)906..1086
Gene:CHAT
Gene Synonym:
Exon (7)1087..1264
Gene:CHAT
Gene Synonym:
Exon (8)1265..1434
Gene:CHAT
Gene Synonym:
Exon (9)1435..1535
Gene:CHAT
Gene Synonym:
Exon (10)1536..1664
Gene:CHAT
Gene Synonym:
Exon (11)1665..1787
Gene:CHAT
Gene Synonym:
Exon (12)1788..1929
Gene:CHAT
Gene Synonym:
Exon (13)1930..1992
Gene:CHAT
Gene Synonym:
Exon (14)1993..2130
Gene:CHAT
Gene Synonym:
Exon (15)2131..2458
Gene:CHAT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001142929
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001142929

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16386
Clone ID Related Accession (Same CDS sequence) NM_001142929 , NM_001142934 , NM_020984 , NM_020985 , NM_020986
Accession Version NM_020985.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D82340.1, AC073366.4, BC130615.1 and AF305907.1. On May 21, 2008 this sequence version replaced gi:119433668. Transcript Variant: This variant (N1) contains alternate 5' exon N and uses an alternate splice site within alternate exon M, and encodes isoform 1 (PMID 9073174). Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF305909.1, BC130615.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 5' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066265.3
CDS336..2228
Misc Feature(1)75..77(+)
Misc Feature(2)372..2141(+)
Exon (1)1..95
Gene:CHAT
Gene Synonym:
Exon (2)96..267
Gene:CHAT
Gene Synonym:
Exon (3)268..368
Gene:CHAT
Gene Synonym:
Exon (4)369..560
Gene:CHAT
Gene Synonym:
Exon (5)561..679
Gene:CHAT
Gene Synonym:
Exon (6)680..733
Gene:CHAT
Gene Synonym:
Exon (7)734..914
Gene:CHAT
Gene Synonym:
Exon (8)915..1092
Gene:CHAT
Gene Synonym:
Exon (9)1093..1262
Gene:CHAT
Gene Synonym:
Exon (10)1263..1363
Gene:CHAT
Gene Synonym:
Exon (11)1364..1492
Gene:CHAT
Gene Synonym:
Exon (12)1493..1615
Gene:CHAT
Gene Synonym:
Exon (13)1616..1757
Gene:CHAT
Gene Synonym:
Exon (14)1758..1820
Gene:CHAT
Gene Synonym:
Exon (15)1821..1958
Gene:CHAT
Gene Synonym:
Exon (16)1959..2286
Gene:CHAT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020985
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020985

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19162
Clone ID Related Accession (Same CDS sequence) NM_020549
Accession Version NM_020549.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073366.4 and AF305907.1. This sequence is a reference standard in the RefSeqGene project. On May 21, 2008 this sequence version replaced gi:119433674. Transcript Variant: This variant (M) contains alternate 5' exon M (PMID 7616604) and uses an upstream start codon. The resulting protein (isoform 2) has a longer N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305907.1, S56138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGGGCTGA GGACAGCGAA GAAGAGGGGG CTTGGGGGAG GGGGGAAATG GAAGAGAGAG 
GAGGGAGGAG GTACAAGAGG AAGGAGAGAA GTGCGGCCAG CTTGCTTTCT CCAGTCGGGT
GGCCGCGGGG ACCCGGGCGA CGTCGGAGGC CCTGCCGGGA ACCCAGGCTG CAGCCCCCAC
CCCCGCGCTG CGACACGCCC CCCACCCCTT CCGGCTCACA CCCCCGCCCA CACTCCTGAG
TGGTGCGGTG CAGCGTCGGC CGAGGCAGCA GAGCCGAGGA GAGCAGGTCC ACACCTCTGC
ATCCCTGCAC CAGGACTCAC CAAGACGCCC ATCCTGGAAA AGGTCCCCCG TAAGATGGCA
GCAAAAACTC CCAGCAGTGA GGAGTCTGGG CTGCCCAAAC TGCCCGTGCC CCCGCTGCAG
CAGACCCTGG CCACGTACCT GCAGTGCATG CGACACTTGG TGTCTGAGGA GCAGTTCAGG
AAGAGCCAGG CCATTGTGCA GCAGTTTGGG GCCCCTGGTG GCCTCGGCGA GACCCTGCAG
CAGAAACTCC TGGAGCGGCA GGAGAAGACA GCCAACTGGG TGTCTGAGTA CTGGCTGAAT
GACATGTATC TCAACAACCG CCTGGCCCTG CCTGTCAACT CCAGCCCTGC CGTGATCTTT
GCTCGGCAGC ACTTCCCTGG CACCGATGAC CAGCTGAGGT TTGCAGCCAG CCTCATCTCT
GGTGTACTCA GCTACAAGGC CCTGCTGGAC AGCCACTCCA TTCCCACTGA CTGTGCCAAA
GGCCAGCTGT CAGGGCAGCC CCTTTGCATG AAGCAATACT ATGGGCTCTT CTCCTCCTAC
CGGCTCCCCG GCCATACCCA GGACACGCTG GTGGCTCAGA ACAGCAGCAT CATGCCGGAG
CCTGAGCACG TCATCGTAGC CTGCTGCAAT CAGTTCTTTG TCTTGGATGT TGTCATTAAT
TTCCGCCGTC TCAGTGAGGG GGATCTGTTC ACTCAGTTGA GAAAGATAGT CAAAATGGCT
TCCAACGAGG ACGAGCGTTT GCCTCCAATT GGCCTGCTGA CGTCTGACGG GAGGAGCGAG
TGGGCCGAGG CCAGGACGGT CCTCGTGAAA GACTCCACCA ACCGGGACTC GCTGGACATG
ATTGAGCGCT GCATCTGCCT TGTATGCCTG GACGCGCCAG GAGGCGTGGA GCTCAGCGAC
ACCCACAGGG CACTCCAGCT CCTTCACGGC GGAGGCTACA GCAAGAACGG GGCCAATCGC
TGGTACGACA AGTCCCTGCA GTTTGTGGTG GGCCGAGACG GCACCTGCGG TGTGGTGTGC
GAACACTCCC CATTCGATGG CATCGTCCTG GTGCAGTGCA CTGAGCATCT GCTCAAGCAC
ATGACGCAGA GCAGCAGGAA GCTGATCCGA GCAGACTCCG TCAGCGAGCT CCCCGCCCCC
CGGAGGCTGC GGTGGAAATG CTCCCCGGAA ATTCAAGGCC ACTTAGCCTC CTCGGCAGAA
AAACTTCAAC GAATAGTAAA GAACCTTGAC TTCATTGTCT ATAAGTTTGA CAACTATGGG
AAAACATTCA TTAAGAAGCA GAAATGCAGC CCTGATGCCT TCATCCAGGT GGCCCTCCAG
CTGGCCTTCT ACAGGCTCCA TCGAAGACTG GTGCCCACCT ACGAGAGCGC GTCCATCCGC
CGATTCCAGG AGGGACGCGT GGACAACATC AGATCGGCCA CTCCAGAGGC ACTGGCTTTT
GTGAGAGCCG TGACTGACCA CAAGGCTGCT GTGCCAGCTT CTGAGAAGCT TCTGCTCCTG
AAGGATGCCA TCCGTGCCCA GACTGCATAC ACAGTCATGG CCATAACAGG GATGGCCATT
GACAACCACC TGCTGGCACT GCGGGAGCTG GCCCGGGCCA TGTGCAAGGA GCTGCCCGAG
ATGTTCATGG ATGAAACCTA CCTGATGAGC AACCGGTTTG TCCTCTCCAC TAGCCAGGTG
CCCACAACCA CGGAGATGTT CTGCTGCTAT GGTCCTGTGG TCCCAAATGG GTATGGTGCC
TGCTACAACC CCCAGCCAGA GACCATCCTT TTCTGCATCT CTAGCTTTCA CAGCTGCAAA
GAGACTTCTT CTAGCAAGTT TGCAAAAGCT GTGGAAGAAA GCCTCATTGA CATGAGAGAC
CTCTGCAGTC TGCTGCCGCC TACTGAGAGC AAGCCATTGG CAACAAAGGA AAAAGCCACG
AGGCCCAGCC AGGGACACCA ACCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_065574.3
CDS154..2400
Misc Feature(1)7..9(+)
Misc Feature(2)544..2313(+)
Misc Feature(3)1711..1749(+)
Exon (1)1..439
Gene:CHAT
Gene Synonym:
Exon (2)440..540
Gene:CHAT
Gene Synonym:
Exon (3)541..732
Gene:CHAT
Gene Synonym:
Exon (4)733..851
Gene:CHAT
Gene Synonym:
Exon (5)852..905
Gene:CHAT
Gene Synonym:
Exon (6)906..1086
Gene:CHAT
Gene Synonym:
Exon (7)1087..1264
Gene:CHAT
Gene Synonym:
Exon (8)1265..1434
Gene:CHAT
Gene Synonym:
Exon (9)1435..1535
Gene:CHAT
Gene Synonym:
Exon (10)1536..1664
Gene:CHAT
Gene Synonym:
Exon (11)1665..1787
Gene:CHAT
Gene Synonym:
Exon (12)1788..1929
Gene:CHAT
Gene Synonym:
Exon (13)1930..1992
Gene:CHAT
Gene Synonym:
Exon (14)1993..2130
Gene:CHAT
Gene Synonym:
Exon (15)2131..2458
Gene:CHAT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020549
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020549

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGGGCTGA GGACAGCGAA GAAGAGGGGG CTTGGGGGAG GGGGGAAATG GAAGAGAGAG 
GAGGGAGGAG GTACAAGAGG AAGGAGAGAA GTGCGGCCAG CTTGCTTTCT CCAGTCGGGT
GGCCGCGGGG ACCCGGGCGA CGTCGGAGGC CCTGCCGGGA ACCCAGGCTG CAGCCCCCAC
CCCCGCGCTG CGACACGCCC CCCACCCCTT CCGGCTCACA CCCCCGCCCA CACTCCTGAG
TGGTGCGGTG CAGCGTCGGC CGAGGCAGCA GAGCCGAGGA GAGCAGGTCC ACACCTCTGC
ATCCCTGCAC CAGGACTCAC CAAGACGCCC ATCCTGGAAA AGGTCCCCCG TAAGATGGCA
GCAAAAACTC CCAGCAGTGA GGAGTCTGGG CTGCCCAAAC TGCCCGTGCC CCCGCTGCAG
CAGACCCTGG CCACGTACCT GCAGTGCATG CGACACTTGG TGTCTGAGGA GCAGTTCAGG
AAGAGCCAGG CCATTGTGCA GCAGTTTGGG GCCCCTGGTG GCCTCGGCGA GACCCTGCAG
CAGAAACTCC TGGAGCGGCA GGAGAAGACA GCCAACTGGG TGTCTGAGTA CTGGCTGAAT
GACATGTATC TCAACAACCG CCTGGCCCTG CCTGTCAACT CCAGCCCTGC CGTGATCTTT
GCTCGGCAGC ACTTCCCTGG CACCGATGAC CAGCTGAGGT TTGCAGCCAG CCTCATCTCT
GGTGTACTCA GCTACAAGGC CCTGCTGGAC AGCCACTCCA TTCCCACTGA CTGTGCCAAA
GGCCAGCTGT CAGGGCAGCC CCTTTGCATG AAGCAATACT ATGGGCTCTT CTCCTCCTAC
CGGCTCCCCG GCCATACCCA GGACACGCTG GTGGCTCAGA ACAGCAGCAT CATGCCGGAG
CCTGAGCACG TCATCGTAGC CTGCTGCAAT CAGTTCTTTG TCTTGGATGT TGTCATTAAT
TTCCGCCGTC TCAGTGAGGG GGATCTGTTC ACTCAGTTGA GAAAGATAGT CAAAATGGCT
TCCAACGAGG ACGAGCGTTT GCCTCCAATT GGCCTGCTGA CGTCTGACGG GAGGAGCGAG
TGGGCCGAGG CCAGGACGGT CCTCGTGAAA GACTCCACCA ACCGGGACTC GCTGGACATG
ATTGAGCGCT GCATCTGCCT TGTATGCCTG GACGCGCCAG GAGGCGTGGA GCTCAGCGAC
ACCCACAGGG CACTCCAGCT CCTTCACGGC GGAGGCTACA GCAAGAACGG GGCCAATCGC
TGGTACGACA AGTCCCTGCA GTTTGTGGTG GGCCGAGACG GCACCTGCGG TGTGGTGTGC
GAACACTCCC CATTCGATGG CATCGTCCTG GTGCAGTGCA CTGAGCATCT GCTCAAGCAC
ATGACGCAGA GCAGCAGGAA GCTGATCCGA GCAGACTCCG TCAGCGAGCT CCCCGCCCCC
CGGAGGCTGC GGTGGAAATG CTCCCCGGAA ATTCAAGGCC ACTTAGCCTC CTCGGCAGAA
AAACTTCAAC GAATAGTAAA GAACCTTGAC TTCATTGTCT ATAAGTTTGA CAACTATGGG
AAAACATTCA TTAAGAAGCA GAAATGCAGC CCTGATGCCT TCATCCAGGT GGCCCTCCAG
CTGGCCTTCT ACAGGCTCCA TCGAAGACTG GTGCCCACCT ACGAGAGCGC GTCCATCCGC
CGATTCCAGG AGGGACGCGT GGACAACATC AGATCGGCCA CTCCAGAGGC ACTGGCTTTT
GTGAGAGCCG TGACTGACCA CAAGGCTGCT GTGCCAGCTT CTGAGAAGCT TCTGCTCCTG
AAGGATGCCA TCCGTGCCCA GACTGCATAC ACAGTCATGG CCATAACAGG GATGGCCATT
GACAACCACC TGCTGGCACT GCGGGAGCTG GCCCGGGCCA TGTGCAAGGA GCTGCCCGAG
ATGTTCATGG ATGAAACCTA CCTGATGAGC AACCGGTTTG TCCTCTCCAC TAGCCAGGTG
CCCACAACCA CGGAGATGTT CTGCTGCTAT GGTCCTGTGG TCCCAAATGG GTATGGTGCC
TGCTACAACC CCCAGCCAGA GACCATCCTT TTCTGCATCT CTAGCTTTCA CAGCTGCAAA
GAGACTTCTT CTAGCAAGTT TGCAAAAGCT GTGGAAGAAA GCCTCATTGA CATGAGAGAC
CTCTGCAGTC TGCTGCCGCC TACTGAGAGC AAGCCATTGG CAACAAAGGA AAAAGCCACG
AGGCCCAGCC AGGGACACCA ACCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16430
Clone ID Related Accession (Same CDS sequence) NM_001142933
Accession Version NM_001142933.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2001bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF305908.2 and AC073366.4. Transcript Variant: This variant (S) contains alternate 5' exons M and S in the 5' coding region, and uses an upstream start codon (PMID 11172068). The resulting protein (isoform 3) has a longer N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305908.2 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGTGGCCGG AATGCAGAGA TGAAGCACTG AGCACAGTAG GTCCACACCT CTGCATCCCT 
GCACCAGGAC TCACCAAGAC GCCCATCCTG GAAAAGGTCC CCCGTAAGAT GGCAGCAAAA
ACTCCCAGCA GTGAGGAGTC TGGGCTGCCC AAACTGCCCG TGCCCCCGCT GCAGCAGACC
CTGGCCACGT ACCTGCAGTG CATGCGACAC TTGGTGTCTG AGGAGCAGTT CAGGAAGAGC
CAGGCCATTG TGCAGCAGTT TGGGGCCCCT GGTGGCCTCG GCGAGACCCT GCAGCAGAAA
CTCCTGGAGC GGCAGGAGAA GACAGCCAAC TGGGTGTCTG AGTACTGGCT GAATGACATG
TATCTCAACA ACCGCCTGGC CCTGCCTGTC AACTCCAGCC CTGCCGTGAT CTTTGCTCGG
CAGCACTTCC CTGGCACCGA TGACCAGCTG AGGTTTGCAG CCAGCCTCAT CTCTGGTGTA
CTCAGCTACA AGGCCCTGCT GGACAGCCAC TCCATTCCCA CTGACTGTGC CAAAGGCCAG
CTGTCAGGGC AGCCCCTTTG CATGAAGCAA TACTATGGGC TCTTCTCCTC CTACCGGCTC
CCCGGCCATA CCCAGGACAC GCTGGTGGCT CAGAACAGCA GCATCATGCC GGAGCCTGAG
CACGTCATCG TAGCCTGCTG CAATCAGTTC TTTGTCTTGG ATGTTGTCAT TAATTTCCGC
CGTCTCAGTG AGGGGGATCT GTTCACTCAG TTGAGAAAGA TAGTCAAAAT GGCTTCCAAC
GAGGACGAGC GTTTGCCTCC AATTGGCCTG CTGACGTCTG ACGGGAGGAG CGAGTGGGCC
GAGGCCAGGA CGGTCCTCGT GAAAGACTCC ACCAACCGGG ACTCGCTGGA CATGATTGAG
CGCTGCATCT GCCTTGTATG CCTGGACGCG CCAGGAGGCG TGGAGCTCAG CGACACCCAC
AGGGCACTCC AGCTCCTTCA CGGCGGAGGC TACAGCAAGA ACGGGGCCAA TCGCTGGTAC
GACAAGTCCC TGCAGTTTGT GGTGGGCCGA GACGGCACCT GCGGTGTGGT GTGCGAACAC
TCCCCATTCG ATGGCATCGT CCTGGTGCAG TGCACTGAGC ATCTGCTCAA GCACATGACG
CAGAGCAGCA GGAAGCTGAT CCGAGCAGAC TCCGTCAGCG AGCTCCCCGC CCCCCGGAGG
CTGCGGTGGA AATGCTCCCC GGAAATTCAA GGCCACTTAG CCTCCTCGGC AGAAAAACTT
CAACGAATAG TAAAGAACCT TGACTTCATT GTCTATAAGT TTGACAACTA TGGGAAAACA
TTCATTAAGA AGCAGAAATG CAGCCCTGAT GCCTTCATCC AGGTGGCCCT CCAGCTGGCC
TTCTACAGGC TCCATCGAAG ACTGGTGCCC ACCTACGAGA GCGCGTCCAT CCGCCGATTC
CAGGAGGGAC GCGTGGACAA CATCAGATCG GCCACTCCAG AGGCACTGGC TTTTGTGAGA
GCCGTGACTG ACCACAAGGC TGCTGTGCCA GCTTCTGAGA AGCTTCTGCT CCTGAAGGAT
GCCATCCGTG CCCAGACTGC ATACACAGTC ATGGCCATAA CAGGGATGGC CATTGACAAC
CACCTGCTGG CACTGCGGGA GCTGGCCCGG GCCATGTGCA AGGAGCTGCC CGAGATGTTC
ATGGATGAAA CCTACCTGAT GAGCAACCGG TTTGTCCTCT CCACTAGCCA GGTGCCCACA
ACCACGGAGA TGTTCTGCTG CTATGGTCCT GTGGTCCCAA ATGGGTATGG TGCCTGCTAC
AACCCCCAGC CAGAGACCAT CCTTTTCTGC ATCTCTAGCT TTCACAGCTG CAAAGAGACT
TCTTCTAGCA AGTTTGCAAA AGCTGTGGAA GAAAGCCTCA TTGACATGAG AGACCTCTGC
AGTCTGCTGC CGCCTACTGA GAGCAAGCCA TTGGCAACAA AGGAAAAAGC CACGAGGCCC
AGCCAGGGAC ACCAACCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001136405.1
CDS470..2470
Misc Feature(1)440..442(+)
Misc Feature(2)614..2383(+)
Exon (1)1..439
Gene:CHAT
Gene Synonym:
Exon (2)440..509
Gene:CHAT
Gene Synonym:
Exon (3)510..610
Gene:CHAT
Gene Synonym:
Exon (4)611..802
Gene:CHAT
Gene Synonym:
Exon (5)803..921
Gene:CHAT
Gene Synonym:
Exon (6)922..975
Gene:CHAT
Gene Synonym:
Exon (7)976..1156
Gene:CHAT
Gene Synonym:
Exon (8)1157..1334
Gene:CHAT
Gene Synonym:
Exon (9)1335..1504
Gene:CHAT
Gene Synonym:
Exon (10)1505..1605
Gene:CHAT
Gene Synonym:
Exon (11)1606..1734
Gene:CHAT
Gene Synonym:
Exon (12)1735..1857
Gene:CHAT
Gene Synonym:
Exon (13)1858..1999
Gene:CHAT
Gene Synonym:
Exon (14)2000..2062
Gene:CHAT
Gene Synonym:
Exon (15)2063..2200
Gene:CHAT
Gene Synonym:
Exon (16)2201..2528
Gene:CHAT
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001142933
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001142933

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGTGGCCGG AATGCAGAGA TGAAGCACTG AGCACAGTAG GTCCACACCT CTGCATCCCT 
GCACCAGGAC TCACCAAGAC GCCCATCCTG GAAAAGGTCC CCCGTAAGAT GGCAGCAAAA
ACTCCCAGCA GTGAGGAGTC TGGGCTGCCC AAACTGCCCG TGCCCCCGCT GCAGCAGACC
CTGGCCACGT ACCTGCAGTG CATGCGACAC TTGGTGTCTG AGGAGCAGTT CAGGAAGAGC
CAGGCCATTG TGCAGCAGTT TGGGGCCCCT GGTGGCCTCG GCGAGACCCT GCAGCAGAAA
CTCCTGGAGC GGCAGGAGAA GACAGCCAAC TGGGTGTCTG AGTACTGGCT GAATGACATG
TATCTCAACA ACCGCCTGGC CCTGCCTGTC AACTCCAGCC CTGCCGTGAT CTTTGCTCGG
CAGCACTTCC CTGGCACCGA TGACCAGCTG AGGTTTGCAG CCAGCCTCAT CTCTGGTGTA
CTCAGCTACA AGGCCCTGCT GGACAGCCAC TCCATTCCCA CTGACTGTGC CAAAGGCCAG
CTGTCAGGGC AGCCCCTTTG CATGAAGCAA TACTATGGGC TCTTCTCCTC CTACCGGCTC
CCCGGCCATA CCCAGGACAC GCTGGTGGCT CAGAACAGCA GCATCATGCC GGAGCCTGAG
CACGTCATCG TAGCCTGCTG CAATCAGTTC TTTGTCTTGG ATGTTGTCAT TAATTTCCGC
CGTCTCAGTG AGGGGGATCT GTTCACTCAG TTGAGAAAGA TAGTCAAAAT GGCTTCCAAC
GAGGACGAGC GTTTGCCTCC AATTGGCCTG CTGACGTCTG ACGGGAGGAG CGAGTGGGCC
GAGGCCAGGA CGGTCCTCGT GAAAGACTCC ACCAACCGGG ACTCGCTGGA CATGATTGAG
CGCTGCATCT GCCTTGTATG CCTGGACGCG CCAGGAGGCG TGGAGCTCAG CGACACCCAC
AGGGCACTCC AGCTCCTTCA CGGCGGAGGC TACAGCAAGA ACGGGGCCAA TCGCTGGTAC
GACAAGTCCC TGCAGTTTGT GGTGGGCCGA GACGGCACCT GCGGTGTGGT GTGCGAACAC
TCCCCATTCG ATGGCATCGT CCTGGTGCAG TGCACTGAGC ATCTGCTCAA GCACATGACG
CAGAGCAGCA GGAAGCTGAT CCGAGCAGAC TCCGTCAGCG AGCTCCCCGC CCCCCGGAGG
CTGCGGTGGA AATGCTCCCC GGAAATTCAA GGCCACTTAG CCTCCTCGGC AGAAAAACTT
CAACGAATAG TAAAGAACCT TGACTTCATT GTCTATAAGT TTGACAACTA TGGGAAAACA
TTCATTAAGA AGCAGAAATG CAGCCCTGAT GCCTTCATCC AGGTGGCCCT CCAGCTGGCC
TTCTACAGGC TCCATCGAAG ACTGGTGCCC ACCTACGAGA GCGCGTCCAT CCGCCGATTC
CAGGAGGGAC GCGTGGACAA CATCAGATCG GCCACTCCAG AGGCACTGGC TTTTGTGAGA
GCCGTGACTG ACCACAAGGC TGCTGTGCCA GCTTCTGAGA AGCTTCTGCT CCTGAAGGAT
GCCATCCGTG CCCAGACTGC ATACACAGTC ATGGCCATAA CAGGGATGGC CATTGACAAC
CACCTGCTGG CACTGCGGGA GCTGGCCCGG GCCATGTGCA AGGAGCTGCC CGAGATGTTC
ATGGATGAAA CCTACCTGAT GAGCAACCGG TTTGTCCTCT CCACTAGCCA GGTGCCCACA
ACCACGGAGA TGTTCTGCTG CTATGGTCCT GTGGTCCCAA ATGGGTATGG TGCCTGCTAC
AACCCCCAGC CAGAGACCAT CCTTTTCTGC ATCTCTAGCT TTCACAGCTG CAAAGAGACT
TCTTCTAGCA AGTTTGCAAA AGCTGTGGAA GAAAGCCTCA TTGACATGAG AGACCTCTGC
AGTCTGCTGC CGCCTACTGA GAGCAAGCCA TTGGCAACAA AGGAAAAAGC CACGAGGCCC
AGCCAGGGAC ACCAACCTTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Nuclear 82-kDa choline acetyltransferase decreases amyloidogenic APP metabolism in neurons from APP/PS1 transgenic mice
Neurobiol. Dis. 69, 32-42 (2014)
Albers S, Inthathirath F, Gill SK, Winick-Ng W, Jaworski E, Wong DY, Gros R and Rylett RJ.


book

Improvement of cognitive function and physical activity of aging mice by human neural stem cells over-expressing choline acetyltransferase
Neurobiol. Aging 34 (11), 2639-2646 (2013)
Park D, Yang YH, Bae DK, Lee SH, Yang G, Kyung J, Kim D, Choi EK, Lee SW, Kim GH, Hong JT, Choi KC, Lee HJ, Kim SU and Kim YB.


book

Determination of allelic expression of SNP rs1880676 in choline acetyltransferase gene in HeLa cells
Neurosci. Lett. 555, 215-219 (2013)
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Human choline acetyltransferase mRNAs with different 5'-region produce a 69-kDa major translation product
Brain Res. Mol. Brain Res. 44 (2), 323-333 (1997)
Misawa H, Matsuura J, Oda Y, Takahashi R and Deguchi T.


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Human choline acetyltransferase gene: localization of alternative first exons
J. Neurosci. Res. 40 (4), 427-438 (1995)
Chireux MA, Le Van Thai A and Weber MJ.


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Congenital Myasthenic Syndromes
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
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A complementary DNA for human choline acetyltransferase induces two forms of enzyme with different molecular weights in cultured cells
Brain Res. Mol. Brain Res. 16 (3-4), 287-294 (1992)
Oda Y, Nakanishi I and Deguchi T.


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Gene expression of mouse choline acetyltransferase. Alternative splicing and identification of a highly active promoter region
J. Biol. Chem. 267 (28), 20392-20399 (1992)
Misawa H, Ishii K and Deguchi T.


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Two mRNAs are transcribed from the human gene for choline acetyltransferase
DNA Cell Biol. 11 (8), 593-603 (1992)
Lorenzi MV, Trinidad AC, Zhang R and Strauss WL.


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Human choline acetyltransferase (CHAT): partial gene sequence and potential control regions
Genomics 12 (2), 412-416 (1992)
Toussaint JL, Geoffroy V, Schmitt M, Werner A, Garnier JM, Simoni P and Kempf J.


 
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