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CHAT cDNA ORF clone, Homo sapiens (human)

Gene Symbol CHAT
Entrez Gene ID 1103
Full Name choline O-acetyltransferase
Synonyms CHOACTASE, CMS1A, CMS1A2, CMS6
General protein information
Preferred Names
choline O-acetyltransferase
Names
choline O-acetyltransferase
choline acetylase
acetyl CoA:choline O-acetyltransferase
NP_001136401.1
NP_001136405.1
NP_001136406.1
NP_065574.3
NP_066264.3
NP_066265.3
NP_066266.3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

10

10q11.2

Summary This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]. lac of sum
Disorder MIM:

118490

Disorder Html: Myasthenic syndrome, congenital, associated with episodic apnea,





The following CHAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16386 NM_020984 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant R, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379.00
OHu16386 NM_001142934 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379.00
OHu16386 NM_020986 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379.00
OHu16386 NM_001142929 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379.00
OHu16386 NM_020985 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $379.00
OHu19162 NM_020549 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu16430 NM_001142933 Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu16386
Accession Version NM_020984.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF305909.1 and AC073366.4. This sequence is a reference standard in the RefSeqGene project. On May 21, 2008 this sequence version replaced gi:119433672. Summary: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]. Transcript Variant: This variant (R) contains alternate 5' exon R (PMID 7616604) and encodes isoform 1. Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305909.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_066264.3
CDS174..2066
Misc Feature(1)63..65(+)
Misc Feature(2)210..1979(+)
Exon (1)1..105
Gene:CHAT
Gene Synonym:
Exon (2)106..206
Gene:CHAT
Gene Synonym:
Exon (3)207..398
Gene:CHAT
Gene Synonym:
Exon (4)399..517
Gene:CHAT
Gene Synonym:
Exon (5)518..571
Gene:CHAT
Gene Synonym:
Exon (6)572..752
Gene:CHAT
Gene Synonym:
Exon (7)753..930
Gene:CHAT
Gene Synonym:
Exon (8)931..1100
Gene:CHAT
Gene Synonym:
Exon (9)1101..1201
Gene:CHAT
Gene Synonym:
Exon (10)1202..1330
Gene:CHAT
Gene Synonym:
Exon (11)1331..1453
Gene:CHAT
Gene Synonym:
Exon (12)1454..1595
Gene:CHAT
Gene Synonym:
Exon (13)1596..1658
Gene:CHAT
Gene Synonym:
Exon (14)1659..1796
Gene:CHAT
Gene Synonym:
Exon (15)1797..2124
Gene:CHAT
Gene Synonym:
Translation
Position Chain Variation Link
9 9 c, g dbSNP:542942211
33 33 c, t dbSNP:186028903
70 70 c, g dbSNP:80200823
115 115 c, t dbSNP:746229284
133 133 c, g dbSNP:149711635
135 135 c, t dbSNP:776042082
145 145 c, t dbSNP:542610160
146 146 a, g dbSNP:79914771
168 168 c, g, t dbSNP:200361897
169 169 a, g, t dbSNP:376641216
176 176 a, g dbSNP:759310217
177 177 a, g dbSNP:3810950
181 181 c, t dbSNP:752726003
193 193 a, g dbSNP:756138129
198 198 a, g dbSNP:777374223
201 201 a, g dbSNP:753288753
204 204 a, t dbSNP:756873370
206 206 c, t dbSNP:143723043
207 207 g, t dbSNP:781596921
210 210 c, t dbSNP:748225279
212 212 g, t dbSNP:769827861
224 224 a, c, g, t dbSNP:757344796
225 225 a, g dbSNP:201479289
227 227 a, g dbSNP:199527987
228 228 c, t dbSNP:376188733
230 230 c, t dbSNP:764063564
232 232 c, g, t dbSNP:557059182
233 233 a, g dbSNP:199675090
236 236 c, g dbSNP:116390167
239 239 a, g dbSNP:758005278
241 241 a, c dbSNP:781476714
253 253 c, g, t dbSNP:766040346
254 254 a, g dbSNP:77296560
257 257 c, t dbSNP:61731734
260 260 a, g dbSNP:753198768
261 261 a, c dbSNP:555796797
270 270 a, c dbSNP:777679246
271 271 a, g dbSNP:202059535
281 281 a, g dbSNP:771258646
293 293 a, g dbSNP:143662656
311 311 c, t dbSNP:746798016
313 313 c, t dbSNP:148155075
325 325 c, t dbSNP:776499844
328 328 a, g dbSNP:370097299
333 333 c, t dbSNP:769800832
335 335 c, t dbSNP:772659997
339 339 a, g dbSNP:762497836
341 341 a, c, t dbSNP:765982984
342 342 c, t dbSNP:759928572
343 343 c, t dbSNP:767712700
344 344 c, t dbSNP:753106995
345 345 a, g dbSNP:374586812
347 347 c, t dbSNP:778476300
348 348 a, g dbSNP:115032198
351 351 a, t dbSNP:757480104
359 359 c, g dbSNP:200143133
366 366 a, c dbSNP:746030420
373 373 a, t dbSNP:757287480
375 375 c, t dbSNP:141111059
376 376 a, c, g dbSNP:747996335
379 379 -, agg dbSNP:758703555
382 382 a, g dbSNP:75011234
395 395 c, t dbSNP:150236872
413 413 c, g dbSNP:773806458
421 421 a, g dbSNP:745437517
424 424 g, t dbSNP:376808313
437 437 a, c dbSNP:775282060
438 438 c, t dbSNP:760936252
439 439 a, c, g dbSNP:764497513
440 440 c, t dbSNP:367783660
441 441 c, t dbSNP:377304752
448 448 c, t dbSNP:121912820
449 449 a, g dbSNP:750447540
450 450 c, g dbSNP:121912815
461 461 c, t dbSNP:758544604
470 470 c, g, t dbSNP:575515952
471 471 a, g dbSNP:751863010
479 479 c, t dbSNP:755203528
480 480 a, g dbSNP:778708569
484 484 a, c, g dbSNP:8178989
494 494 c, t dbSNP:267602510
497 497 c, t dbSNP:142740451
498 498 a, g dbSNP:778954064
501 501 a, t dbSNP:745367841
503 503 c, t dbSNP:79030468
504 504 a, g dbSNP:774877358
507 507 c, g dbSNP:746608168
522 522 a, g dbSNP:748516964
526 526 c, t dbSNP:770250348
530 530 c, g dbSNP:78925077
531 531 c, g dbSNP:375606885
543 543 a, g dbSNP:137899400
546 546 c, t dbSNP:8178990
556 556 a, g dbSNP:774436099
559 559 c, t dbSNP:561562136
560 560 c, g dbSNP:767727254
563 563 c, g dbSNP:752976973
564 564 c, g dbSNP:115510708
566 566 a, g dbSNP:765018013
573 573 c, t dbSNP:376994468
586 586 c, t dbSNP:754977595
602 602 c, t dbSNP:767065663
606 606 c, t dbSNP:74133815
608 608 a, c, g dbSNP:114090981
613 613 a, g dbSNP:777336258
617 617 a, g dbSNP:142237923
619 619 c, t dbSNP:749662741
625 625 g, t dbSNP:757624145
631 631 a, g dbSNP:779266134
639 639 g, t dbSNP:746310436
647 647 c, g dbSNP:772600687
650 650 -, ctc dbSNP:560648873
653 653 c, t dbSNP:775502083
660 660 c, t dbSNP:747108035
662 662 c, g dbSNP:768892260
669 669 a, g dbSNP:146236256
677 677 c, t dbSNP:200423662
683 683 c, t dbSNP:766217426
685 685 c, t dbSNP:139352363
686 686 a, g, t dbSNP:574542737
687 687 c, t dbSNP:752229226
705 705 a, g dbSNP:141599748
714 714 c, t dbSNP:540899575
715 715 c, t dbSNP:868749
716 716 a, g dbSNP:756942813
722 722 c, t dbSNP:113897064
723 723 c, g dbSNP:746255012
728 728 c, g, t dbSNP:76570508
729 729 a, g dbSNP:116362950
732 732 a, g dbSNP:370481031
733 733 c, g, t dbSNP:75466054
735 735 a, g dbSNP:551219437
737 737 -, agcc dbSNP:769980623
738 738 a, g dbSNP:776785129
739 739 c, t dbSNP:748250869
748 748 a, g dbSNP:149976821
769 769 c, t dbSNP:778178080
783 783 c, t dbSNP:147815999
786 786 c, t dbSNP:771938918
787 787 a, c, g dbSNP:200176236
793 793 c, g dbSNP:768665020
799 799 a, g dbSNP:546158744
800 800 a, g, t dbSNP:201359694
801 801 a, g dbSNP:370202834
803 803 c, t dbSNP:750133075
814 814 a, g dbSNP:762605295
825 825 a, t dbSNP:766585447
826 826 c, t dbSNP:121912823
835 835 c, t dbSNP:375439849
844 844 a, g dbSNP:781745178
845 845 c, t dbSNP:752685760
846 846 a, g dbSNP:778837182
851 851 c, t dbSNP:145203976
852 852 a, g dbSNP:200776329
855 855 c, t dbSNP:182935725
856 856 a, g dbSNP:779668516
857 857 c, t dbSNP:367961073
863 863 a, t dbSNP:200009054
864 864 c, g dbSNP:776699190
869 869 a, t dbSNP:74340719
878 878 a, g dbSNP:747645217
880 880 c, t dbSNP:769234940
881 881 a, g dbSNP:529337162
887 887 c, t dbSNP:748193892
888 888 a, g dbSNP:61731735
893 893 a, g dbSNP:371905802
896 896 c, t dbSNP:759981661
897 897 a, g dbSNP:767903393
902 902 a, g dbSNP:753310881
905 905 c, g, t dbSNP:374870408
906 906 a, g dbSNP:75262191
908 908 a, g dbSNP:757402644
910 910 c, t dbSNP:779237093
916 916 a, c, t dbSNP:533289708
917 917 a, g dbSNP:780860401
923 923 c, t dbSNP:551349781
924 924 a, g, t dbSNP:769754418
941 941 c, t dbSNP:61115650
942 942 a, c, t dbSNP:141794970
943 943 a, g dbSNP:201616704
950 950 a, g dbSNP:115126024
954 954 c, g dbSNP:115212829
958 958 c, t dbSNP:778441042
961 961 c, t dbSNP:372758559
966 966 c, t dbSNP:771822812
967 967 a, g dbSNP:779919147
980 980 c, t dbSNP:562306374
981 981 c, g dbSNP:747366078
984 984 c, t dbSNP:769085306
986 986 c, t dbSNP:777142490
992 992 c, t dbSNP:141690626
993 993 a, g dbSNP:115877658
994 994 c, t dbSNP:114932900
995 995 a, g dbSNP:200321426
996 996 c, t dbSNP:201553605
1005 1005 a, g dbSNP:536359684
1016 1016 c, t dbSNP:554276268
1017 1017 a, g dbSNP:8178991
1021 1021 a, c dbSNP:539884711
1030 1030 a, c, t dbSNP:192592698
1046 1046 c, t dbSNP:578201320
1047 1047 a, g, t dbSNP:749948399
1049 1049 c, t dbSNP:779724822
1050 1050 a, g, t dbSNP:746595362
1053 1053 a, g dbSNP:781569427
1055 1055 a, c dbSNP:748510157
1058 1058 c, t dbSNP:770303527
1067 1067 c, t dbSNP:116071049
1072 1072 c, t dbSNP:376230019
1077 1077 c, t dbSNP:121912822
1079 1079 c, t dbSNP:770937861
1085 1085 c, t dbSNP:201381950
1086 1086 a, g dbSNP:759707536
1094 1094 a, c dbSNP:767778694
1102 1102 c, t dbSNP:774394630
1107 1107 a, g dbSNP:745991038
1114 1114 a, g dbSNP:377494035
1118 1118 c, t dbSNP:146284420
1119 1119 a, g dbSNP:115964813
1126 1126 a, g dbSNP:139416405
1127 1127 c, t dbSNP:764875843
1128 1128 a, g dbSNP:562560467
1138 1138 a, g dbSNP:762936544
1139 1139 c, t dbSNP:766466820
1140 1140 a, g dbSNP:121912816
1142 1142 a, g dbSNP:759183550
1147 1147 -, c dbSNP:768533400
1149 1149 c, g dbSNP:767180792
1154 1154 a, c, t dbSNP:752229820
1157 1157 c, t dbSNP:777957584
1163 1163 c, t dbSNP:754253168
1164 1164 a, g dbSNP:757733319
1170 1170 c, g dbSNP:779466347
1179 1179 a, g dbSNP:745938985
1191 1191 c, t dbSNP:76014951
1194 1194 a, c, g dbSNP:769398642
1200 1200 a, g dbSNP:4838544
1202 1202 a, g dbSNP:370918939
1203 1203 a, g dbSNP:781625888
1204 1204 c, t dbSNP:538478136
1205 1205 a, g dbSNP:770792319
1210 1210 a, g dbSNP:148525631
1227 1227 a, c, t dbSNP:142889639
1228 1228 a, g dbSNP:774834313
1229 1229 a, c dbSNP:760300737
1238 1238 c, t dbSNP:200175587
1239 1239 a, g dbSNP:371719364
1240 1240 c, t dbSNP:761462857
1242 1242 a, t dbSNP:765549293
1244 1244 a, c, t dbSNP:553771451
1245 1245 a, g dbSNP:766947649
1254 1254 -, c dbSNP:773268576
1254 1254 a, g dbSNP:3793795
1260 1260 c, g, t dbSNP:751583653
1261 1261 a, g dbSNP:202125860
1262 1262 a, g dbSNP:748314389
1263 1263 a, c, g dbSNP:121912818
1269 1269 a, c, t dbSNP:778801148
1270 1270 a, g dbSNP:771901229
1271 1271 a, c, g dbSNP:775557792
1273 1273 a, g dbSNP:768087857
1278 1278 a, t dbSNP:375424189
1279 1279 g, t dbSNP:201293521
1285 1285 c, t dbSNP:764921945
1286 1286 a, g dbSNP:558057516
1287 1287 -, ga dbSNP:760039781
1289 1289 a, c dbSNP:773420869
1294 1294 a, c dbSNP:763439208
1304 1304 a, g dbSNP:151031033
1309 1309 c, t dbSNP:141878259
1312 1312 c, t dbSNP:121912821
1313 1313 a, g dbSNP:139722660
1314 1314 a, g dbSNP:767664496
1316 1316 a, g dbSNP:752844751
1322 1322 a, c, t dbSNP:756381050
1329 1329 c, t dbSNP:371470622
1330 1330 a, g dbSNP:200335347
1335 1335 g, t dbSNP:121912817
1347 1347 g, t dbSNP:752482024
1349 1349 c, t dbSNP:558298529
1354 1354 c, t dbSNP:769232976
1358 1358 a, c dbSNP:777148519
1360 1360 a, g dbSNP:144918120
1383 1383 a, g dbSNP:770511614
1385 1385 a, c, g dbSNP:74433353
1386 1386 c, t dbSNP:760016941
1389 1389 a, g dbSNP:75981983
1424 1424 c, g dbSNP:772695708
1427 1427 a, g dbSNP:758135155
1436 1436 c, g dbSNP:776084200
1454 1454 g, t dbSNP:201857639
1460 1460 a, c, t dbSNP:8178992
1461 1461 c, t dbSNP:369251527
1462 1462 a, g dbSNP:141881680
1465 1465 g, t dbSNP:756056022
1466 1466 a, g dbSNP:763978618
1473 1473 c, t dbSNP:147079245
1476 1476 a, c dbSNP:756665776
1480 1480 a, g dbSNP:778572568
1481 1481 c, t dbSNP:145370753
1482 1482 a, g dbSNP:757303526
1487 1487 c, t dbSNP:55702495
1488 1488 a, g dbSNP:372760913
1489 1489 c, t dbSNP:769204680
1490 1490 a, g dbSNP:777066390
1493 1493 c, t dbSNP:7073028
1494 1494 a, g dbSNP:202040717
1497 1497 c, t dbSNP:773307141
1498 1498 a, g dbSNP:121912819
1501 1501 a, g dbSNP:80097077
1515 1515 c, t dbSNP:368957556
1517 1517 c, t dbSNP:760452753
1518 1518 a, g dbSNP:764077878
1522 1522 -, aca dbSNP:139324199
1534 1534 c, g dbSNP:753652169
1535 1535 a, g dbSNP:374000183
1551 1551 c, t dbSNP:764791712
1554 1554 a, g dbSNP:750002983
1564 1564 c, g dbSNP:758095810
1565 1565 a, g dbSNP:779923095
1568 1568 c, g, t dbSNP:201580702
1569 1569 a, g dbSNP:547073881
1586 1586 c, t dbSNP:781621361
1587 1587 g, t dbSNP:376947247
1590 1590 a, g dbSNP:201485243
1603 1603 a, t dbSNP:190968891
1619 1619 a, g, t dbSNP:373259575
1621 1621 a, c dbSNP:776260066
1624 1624 a, g dbSNP:761771110
1625 1625 c, t dbSNP:111795945
1633 1633 a, g dbSNP:199926163
1642 1642 a, c dbSNP:773228076
1646 1646 a, g dbSNP:762991238
1647 1647 a, t dbSNP:765943808
1653 1653 c, g dbSNP:751100162
1662 1662 a, g dbSNP:759151717
1672 1672 c, t dbSNP:767062466
1699 1699 c, t dbSNP:775066227
1700 1700 c, g dbSNP:760324497
1701 1701 a, c, t dbSNP:116097791
1702 1702 a, g dbSNP:114545628
1711 1711 c, t dbSNP:765840442
1713 1713 c, t dbSNP:141127966
1714 1714 a, g dbSNP:758448736
1717 1717 c, t dbSNP:780006014
1721 1721 a, g dbSNP:747198406
1724 1724 a, c dbSNP:145243180
1726 1726 a, g dbSNP:755269798
1728 1728 a, g dbSNP:777684375
1730 1730 a, g dbSNP:749133071
1736 1736 c, g, t dbSNP:370200905
1737 1737 a, g dbSNP:774403163
1749 1749 c, g dbSNP:745804589
1753 1753 a, g dbSNP:375665108
1754 1754 a, c dbSNP:775011277
1758 1758 c, t dbSNP:760299925
1763 1763 a, g dbSNP:368690834
1773 1773 c, t dbSNP:776977900
1774 1774 a, g dbSNP:116628504
1775 1775 g, t dbSNP:765535877
1790 1790 c, t dbSNP:368254411
1792 1792 a, g dbSNP:138105397
1794 1794 a, c dbSNP:766430404
1811 1811 a, g dbSNP:767717703
1814 1814 a, g, t dbSNP:752896534
1822 1822 a, g dbSNP:778719316
1853 1853 c, t dbSNP:750226582
1859 1859 c, g dbSNP:758352141
1865 1865 c, t dbSNP:780026522
1868 1868 c, t dbSNP:758454149
1869 1869 c, t dbSNP:550322570
1885 1885 a, t dbSNP:768089988
1886 1886 c, t dbSNP:3793801
1895 1895 c, t dbSNP:747791343
1900 1900 c, g dbSNP:201439531
1902 1902 a, g dbSNP:769377082
1903 1903 c, g dbSNP:773591109
1924 1924 -, ctt dbSNP:750942168
1946 1946 a, c dbSNP:145995632
1952 1952 a, g dbSNP:747261452
1958 1958 a, c dbSNP:771548584
1960 1960 a, g dbSNP:535850567
1961 1961 a, c dbSNP:114208185
1964 1964 c, t dbSNP:760123165
1988 1988 c, t dbSNP:767664583
1989 1989 a, c dbSNP:752740939
1990 1990 g, t dbSNP:760920908
1991 1991 a, g dbSNP:566628025
1996 1996 c, g, t dbSNP:79414242
1997 1997 a, g dbSNP:77144546
2002 2002 c, t dbSNP:74334349
2004 2004 a, g dbSNP:751461280
2006 2006 a, g dbSNP:754935493
2024 2024 -, a dbSNP:768461054
2028 2028 a, g dbSNP:780662703
2031 2031 a, g dbSNP:142285373
2038 2038 c, t dbSNP:755783618
2041 2041 a, g dbSNP:114719193
2045 2045 c, g dbSNP:748981265
2050 2050 a, g dbSNP:771354440
2061 2061 c, g dbSNP:774886016
2062 2062 a, c dbSNP:746327923
2068 2068 c, t dbSNP:772755011
2071 2071 c, t dbSNP:374303993
2075 2075 a, g dbSNP:775635830
2080 2080 g, t dbSNP:760725539
2084 2084 a, c dbSNP:764248770
2085 2085 a, c dbSNP:776653200
2086 2086 c, t dbSNP:762055920
2087 2087 a, c dbSNP:766117753
2088 2088 c, t dbSNP:751408195
2089 2089 c, g dbSNP:578155039
2091 2091 a, c dbSNP:754875240
2094 2094 -, c dbSNP:145178233
2094 2094 a, c dbSNP:767416437
2096 2096 c, g dbSNP:759489601
2097 2097 c, t dbSNP:752234656
2100 2100 a, c dbSNP:199684404
2110 2110 -, agcca dbSNP:780315825
2118 2118 c, t dbSNP:755519156

Target ORF information:

RefSeq Version NM_020984
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant R, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020984

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16386
Accession Version NM_001142934.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF305908.2 and AC073366.4. Summary: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]. Transcript Variant: This variant (S) contains alternate 5' exons M and S in the 5' coding region but uses a downstream start codon and encodes isoform 1 (PMID 11172068). Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305908.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1337937) ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001136406.1
CDS578..2470
Misc Feature(1)440..442(+)
Misc Feature(2)614..2383(+)
Exon (1)1..439
Gene:CHAT
Gene Synonym:
Exon (2)440..509
Gene:CHAT
Gene Synonym:
Exon (3)510..610
Gene:CHAT
Gene Synonym:
Exon (4)611..802
Gene:CHAT
Gene Synonym:
Exon (5)803..921
Gene:CHAT
Gene Synonym:
Exon (6)922..975
Gene:CHAT
Gene Synonym:
Exon (7)976..1156
Gene:CHAT
Gene Synonym:
Exon (8)1157..1334
Gene:CHAT
Gene Synonym:
Exon (9)1335..1504
Gene:CHAT
Gene Synonym:
Exon (10)1505..1605
Gene:CHAT
Gene Synonym:
Exon (11)1606..1734
Gene:CHAT
Gene Synonym:
Exon (12)1735..1857
Gene:CHAT
Gene Synonym:
Exon (13)1858..1999
Gene:CHAT
Gene Synonym:
Exon (14)2000..2062
Gene:CHAT
Gene Synonym:
Exon (15)2063..2200
Gene:CHAT
Gene Synonym:
Exon (16)2201..2528
Gene:CHAT
Gene Synonym:
Translation
Position Chain Variation Link
16 16 a, g dbSNP:764161559
51 51 c, g dbSNP:563585126
79 79 a, g dbSNP:755878487
110 110 a, c, g dbSNP:7903315
129 129 c, t dbSNP:751007736
134 134 g, t dbSNP:758531527
137 137 a, g dbSNP:77152496
144 144 g, t dbSNP:7923716
148 148 g, t dbSNP:755091583
168 168 a, c dbSNP:781493867
169 169 a, g dbSNP:749073862
174 174 g, t dbSNP:79528320
178 178 a, g dbSNP:587780314
183 183 c, g dbSNP:528479799
188 188 g, t dbSNP:770749789
190 190 a, g dbSNP:750228985
192 192 a, g dbSNP:12413661
198 198 a, g dbSNP:778795905
206 206 -, ag dbSNP:531450737
209 209 c, g dbSNP:546932446
230 230 c, g dbSNP:374430783
237 237 g, t dbSNP:745753124
238 238 a, c dbSNP:772025588
248 248 a, g dbSNP:377156965
267 267 c, g dbSNP:746540866
269 269 a, c dbSNP:370609858
270 270 c, g dbSNP:768179285
272 272 g, t dbSNP:776411377
294 294 c, g dbSNP:3810948
301 301 g, t dbSNP:765746009
306 306 a, t dbSNP:773735383
309 309 -, a dbSNP:745552600
327 327 a, c dbSNP:572002174
329 329 c, t dbSNP:766916844
340 340 g, t dbSNP:751539916
362 362 c, t dbSNP:755147781
375 375 c, t dbSNP:767600029
380 380 c, t dbSNP:752962530
386 386 a, c dbSNP:550874261
391 391 c, g dbSNP:756348058
405 405 a, t dbSNP:112079336
409 409 -, cggc dbSNP:761691250
411 411 c, g dbSNP:778636468
415 415 a, g dbSNP:372018292
428 428 c, t dbSNP:372298555
429 429 a, g dbSNP:745665140
443 443 a, g dbSNP:759508399
450 450 c, t dbSNP:78095850
451 451 a, c dbSNP:371673693
455 455 a, c, t dbSNP:752801376
459 459 c, g dbSNP:764311143
468 468 a, g dbSNP:750204722
474 474 g, t dbSNP:376040545
477 477 a, c, t dbSNP:41306415
478 478 a, g dbSNP:374523860
488 488 a, g dbSNP:1880676
490 490 g, t dbSNP:747668520
497 497 a, c dbSNP:370690022
498 498 c, t dbSNP:777629256
500 500 a, c dbSNP:749614248
505 505 a, g dbSNP:771372324
519 519 c, t dbSNP:746229284
537 537 c, g dbSNP:149711635
539 539 c, t dbSNP:776042082
549 549 c, t dbSNP:542610160
550 550 a, g dbSNP:79914771
572 572 c, g, t dbSNP:200361897
573 573 a, g, t dbSNP:376641216
580 580 a, g dbSNP:759310217
581 581 a, g dbSNP:3810950
585 585 c, t dbSNP:752726003
597 597 a, g dbSNP:756138129
602 602 a, g dbSNP:777374223
605 605 a, g dbSNP:753288753
608 608 a, t dbSNP:756873370
610 610 c, t dbSNP:143723043
611 611 g, t dbSNP:781596921
614 614 c, t dbSNP:748225279
616 616 g, t dbSNP:769827861
628 628 a, c, g, t dbSNP:757344796
629 629 a, g dbSNP:201479289
631 631 a, g dbSNP:199527987
632 632 c, t dbSNP:376188733
634 634 c, t dbSNP:764063564
636 636 c, g, t dbSNP:557059182
637 637 a, g dbSNP:199675090
640 640 c, g dbSNP:116390167
643 643 a, g dbSNP:758005278
645 645 a, c dbSNP:781476714
657 657 c, g, t dbSNP:766040346
658 658 a, g dbSNP:77296560
661 661 c, t dbSNP:61731734
664 664 a, g dbSNP:753198768
665 665 a, c dbSNP:555796797
674 674 a, c dbSNP:777679246
675 675 a, g dbSNP:202059535
685 685 a, g dbSNP:771258646
697 697 a, g dbSNP:143662656
715 715 c, t dbSNP:746798016
717 717 c, t dbSNP:148155075
729 729 c, t dbSNP:776499844
732 732 a, g dbSNP:370097299
737 737 c, t dbSNP:769800832
739 739 c, t dbSNP:772659997
743 743 a, g dbSNP:762497836
745 745 a, c, t dbSNP:765982984
746 746 c, t dbSNP:759928572
747 747 c, t dbSNP:767712700
748 748 c, t dbSNP:753106995
749 749 a, g dbSNP:374586812
751 751 c, t dbSNP:778476300
752 752 a, g dbSNP:115032198
755 755 a, t dbSNP:757480104
763 763 c, g dbSNP:200143133
770 770 a, c dbSNP:746030420
777 777 a, t dbSNP:757287480
779 779 c, t dbSNP:141111059
780 780 a, c, g dbSNP:747996335
783 783 -, agg dbSNP:758703555
786 786 a, g dbSNP:75011234
799 799 c, t dbSNP:150236872
817 817 c, g dbSNP:773806458
825 825 a, g dbSNP:745437517
828 828 g, t dbSNP:376808313
841 841 a, c dbSNP:775282060
842 842 c, t dbSNP:760936252
843 843 a, c, g dbSNP:764497513
844 844 c, t dbSNP:367783660
845 845 c, t dbSNP:377304752
852 852 c, t dbSNP:121912820
853 853 a, g dbSNP:750447540
854 854 c, g dbSNP:121912815
865 865 c, t dbSNP:758544604
874 874 c, g, t dbSNP:575515952
875 875 a, g dbSNP:751863010
883 883 c, t dbSNP:755203528
884 884 a, g dbSNP:778708569
888 888 a, c, g dbSNP:8178989
898 898 c, t dbSNP:267602510
901 901 c, t dbSNP:142740451
902 902 a, g dbSNP:778954064
905 905 a, t dbSNP:745367841
907 907 c, t dbSNP:79030468
908 908 a, g dbSNP:774877358
911 911 c, g dbSNP:746608168
926 926 a, g dbSNP:748516964
930 930 c, t dbSNP:770250348
934 934 c, g dbSNP:78925077
935 935 c, g dbSNP:375606885
947 947 a, g dbSNP:137899400
950 950 c, t dbSNP:8178990
960 960 a, g dbSNP:774436099
963 963 c, t dbSNP:561562136
964 964 c, g dbSNP:767727254
967 967 c, g dbSNP:752976973
968 968 c, g dbSNP:115510708
970 970 a, g dbSNP:765018013
977 977 c, t dbSNP:376994468
990 990 c, t dbSNP:754977595
1006 1006 c, t dbSNP:767065663
1010 1010 c, t dbSNP:74133815
1012 1012 a, c, g dbSNP:114090981
1017 1017 a, g dbSNP:777336258
1021 1021 a, g dbSNP:142237923
1023 1023 c, t dbSNP:749662741
1029 1029 g, t dbSNP:757624145
1035 1035 a, g dbSNP:779266134
1043 1043 g, t dbSNP:746310436
1051 1051 c, g dbSNP:772600687
1054 1054 -, ctc dbSNP:560648873
1057 1057 c, t dbSNP:775502083
1064 1064 c, t dbSNP:747108035
1066 1066 c, g dbSNP:768892260
1073 1073 a, g dbSNP:146236256
1081 1081 c, t dbSNP:200423662
1087 1087 c, t dbSNP:766217426
1089 1089 c, t dbSNP:139352363
1090 1090 a, g, t dbSNP:574542737
1091 1091 c, t dbSNP:752229226
1109 1109 a, g dbSNP:141599748
1118 1118 c, t dbSNP:540899575
1119 1119 c, t dbSNP:868749
1120 1120 a, g dbSNP:756942813
1126 1126 c, t dbSNP:113897064
1127 1127 c, g dbSNP:746255012
1132 1132 c, g, t dbSNP:76570508
1133 1133 a, g dbSNP:116362950
1136 1136 a, g dbSNP:370481031
1137 1137 c, g, t dbSNP:75466054
1139 1139 a, g dbSNP:551219437
1141 1141 -, agcc dbSNP:769980623
1142 1142 a, g dbSNP:776785129
1143 1143 c, t dbSNP:748250869
1152 1152 a, g dbSNP:149976821
1173 1173 c, t dbSNP:778178080
1187 1187 c, t dbSNP:147815999
1190 1190 c, t dbSNP:771938918
1191 1191 a, c, g dbSNP:200176236
1197 1197 c, g dbSNP:768665020
1203 1203 a, g dbSNP:546158744
1204 1204 a, g, t dbSNP:201359694
1205 1205 a, g dbSNP:370202834
1207 1207 c, t dbSNP:750133075
1218 1218 a, g dbSNP:762605295
1229 1229 a, t dbSNP:766585447
1230 1230 c, t dbSNP:121912823
1239 1239 c, t dbSNP:375439849
1248 1248 a, g dbSNP:781745178
1249 1249 c, t dbSNP:752685760
1250 1250 a, g dbSNP:778837182
1255 1255 c, t dbSNP:145203976
1256 1256 a, g dbSNP:200776329
1259 1259 c, t dbSNP:182935725
1260 1260 a, g dbSNP:779668516
1261 1261 c, t dbSNP:367961073
1267 1267 a, t dbSNP:200009054
1268 1268 c, g dbSNP:776699190
1273 1273 a, t dbSNP:74340719
1282 1282 a, g dbSNP:747645217
1284 1284 c, t dbSNP:769234940
1285 1285 a, g dbSNP:529337162
1291 1291 c, t dbSNP:748193892
1292 1292 a, g dbSNP:61731735
1297 1297 a, g dbSNP:371905802
1300 1300 c, t dbSNP:759981661
1301 1301 a, g dbSNP:767903393
1306 1306 a, g dbSNP:753310881
1309 1309 c, g, t dbSNP:374870408
1310 1310 a, g dbSNP:75262191
1312 1312 a, g dbSNP:757402644
1314 1314 c, t dbSNP:779237093
1320 1320 a, c, t dbSNP:533289708
1321 1321 a, g dbSNP:780860401
1327 1327 c, t dbSNP:551349781
1328 1328 a, g, t dbSNP:769754418
1345 1345 c, t dbSNP:61115650
1346 1346 a, c, t dbSNP:141794970
1347 1347 a, g dbSNP:201616704
1354 1354 a, g dbSNP:115126024
1358 1358 c, g dbSNP:115212829
1362 1362 c, t dbSNP:778441042
1365 1365 c, t dbSNP:372758559
1370 1370 c, t dbSNP:771822812
1371 1371 a, g dbSNP:779919147
1384 1384 c, t dbSNP:562306374
1385 1385 c, g dbSNP:747366078
1388 1388 c, t dbSNP:769085306
1390 1390 c, t dbSNP:777142490
1396 1396 c, t dbSNP:141690626
1397 1397 a, g dbSNP:115877658
1398 1398 c, t dbSNP:114932900
1399 1399 a, g dbSNP:200321426
1400 1400 c, t dbSNP:201553605
1409 1409 a, g dbSNP:536359684
1420 1420 c, t dbSNP:554276268
1421 1421 a, g dbSNP:8178991
1425 1425 a, c dbSNP:539884711
1434 1434 a, c, t dbSNP:192592698
1450 1450 c, t dbSNP:578201320
1451 1451 a, g, t dbSNP:749948399
1453 1453 c, t dbSNP:779724822
1454 1454 a, g, t dbSNP:746595362
1457 1457 a, g dbSNP:781569427
1459 1459 a, c dbSNP:748510157
1462 1462 c, t dbSNP:770303527
1471 1471 c, t dbSNP:116071049
1476 1476 c, t dbSNP:376230019
1481 1481 c, t dbSNP:121912822
1483 1483 c, t dbSNP:770937861
1489 1489 c, t dbSNP:201381950
1490 1490 a, g dbSNP:759707536
1498 1498 a, c dbSNP:767778694
1506 1506 c, t dbSNP:774394630
1511 1511 a, g dbSNP:745991038
1518 1518 a, g dbSNP:377494035
1522 1522 c, t dbSNP:146284420
1523 1523 a, g dbSNP:115964813
1530 1530 a, g dbSNP:139416405
1531 1531 c, t dbSNP:764875843
1532 1532 a, g dbSNP:562560467
1542 1542 a, g dbSNP:762936544
1543 1543 c, t dbSNP:766466820
1544 1544 a, g dbSNP:121912816
1546 1546 a, g dbSNP:759183550
1551 1551 -, c dbSNP:768533400
1553 1553 c, g dbSNP:767180792
1558 1558 a, c, t dbSNP:752229820
1561 1561 c, t dbSNP:777957584
1567 1567 c, t dbSNP:754253168
1568 1568 a, g dbSNP:757733319
1574 1574 c, g dbSNP:779466347
1583 1583 a, g dbSNP:745938985
1595 1595 c, t dbSNP:76014951
1598 1598 a, c, g dbSNP:769398642
1604 1604 a, g dbSNP:4838544
1606 1606 a, g dbSNP:370918939
1607 1607 a, g dbSNP:781625888
1608 1608 c, t dbSNP:538478136
1609 1609 a, g dbSNP:770792319
1614 1614 a, g dbSNP:148525631
1631 1631 a, c, t dbSNP:142889639
1632 1632 a, g dbSNP:774834313
1633 1633 a, c dbSNP:760300737
1642 1642 c, t dbSNP:200175587
1643 1643 a, g dbSNP:371719364
1644 1644 c, t dbSNP:761462857
1646 1646 a, t dbSNP:765549293
1648 1648 a, c, t dbSNP:553771451
1649 1649 a, g dbSNP:766947649
1658 1658 -, c dbSNP:773268576
1658 1658 a, g dbSNP:3793795
1664 1664 c, g, t dbSNP:751583653
1665 1665 a, g dbSNP:202125860
1666 1666 a, g dbSNP:748314389
1667 1667 a, c, g dbSNP:121912818
1673 1673 a, c, t dbSNP:778801148
1674 1674 a, g dbSNP:771901229
1675 1675 a, c, g dbSNP:775557792
1677 1677 a, g dbSNP:768087857
1682 1682 a, t dbSNP:375424189
1683 1683 g, t dbSNP:201293521
1689 1689 c, t dbSNP:764921945
1690 1690 a, g dbSNP:558057516
1691 1691 -, ga dbSNP:760039781
1693 1693 a, c dbSNP:773420869
1698 1698 a, c dbSNP:763439208
1708 1708 a, g dbSNP:151031033
1713 1713 c, t dbSNP:141878259
1716 1716 c, t dbSNP:121912821
1717 1717 a, g dbSNP:139722660
1718 1718 a, g dbSNP:767664496
1720 1720 a, g dbSNP:752844751
1726 1726 a, c, t dbSNP:756381050
1733 1733 c, t dbSNP:371470622
1734 1734 a, g dbSNP:200335347
1739 1739 g, t dbSNP:121912817
1751 1751 g, t dbSNP:752482024
1753 1753 c, t dbSNP:558298529
1758 1758 c, t dbSNP:769232976
1762 1762 a, c dbSNP:777148519
1764 1764 a, g dbSNP:144918120
1787 1787 a, g dbSNP:770511614
1789 1789 a, c, g dbSNP:74433353
1790 1790 c, t dbSNP:760016941
1793 1793 a, g dbSNP:75981983
1828 1828 c, g dbSNP:772695708
1831 1831 a, g dbSNP:758135155
1840 1840 c, g dbSNP:776084200
1858 1858 g, t dbSNP:201857639
1864 1864 a, c, t dbSNP:8178992
1865 1865 c, t dbSNP:369251527
1866 1866 a, g dbSNP:141881680
1869 1869 g, t dbSNP:756056022
1870 1870 a, g dbSNP:763978618
1877 1877 c, t dbSNP:147079245
1880 1880 a, c dbSNP:756665776
1884 1884 a, g dbSNP:778572568
1885 1885 c, t dbSNP:145370753
1886 1886 a, g dbSNP:757303526
1891 1891 c, t dbSNP:55702495
1892 1892 a, g dbSNP:372760913
1893 1893 c, t dbSNP:769204680
1894 1894 a, g dbSNP:777066390
1897 1897 c, t dbSNP:7073028
1898 1898 a, g dbSNP:202040717
1901 1901 c, t dbSNP:773307141
1902 1902 a, g dbSNP:121912819
1905 1905 a, g dbSNP:80097077
1919 1919 c, t dbSNP:368957556
1921 1921 c, t dbSNP:760452753
1922 1922 a, g dbSNP:764077878
1926 1926 -, aca dbSNP:139324199
1938 1938 c, g dbSNP:753652169
1939 1939 a, g dbSNP:374000183
1955 1955 c, t dbSNP:764791712
1958 1958 a, g dbSNP:750002983
1968 1968 c, g dbSNP:758095810
1969 1969 a, g dbSNP:779923095
1972 1972 c, g, t dbSNP:201580702
1973 1973 a, g dbSNP:547073881
1990 1990 c, t dbSNP:781621361
1991 1991 g, t dbSNP:376947247
1994 1994 a, g dbSNP:201485243
2007 2007 a, t dbSNP:190968891
2023 2023 a, g, t dbSNP:373259575
2025 2025 a, c dbSNP:776260066
2028 2028 a, g dbSNP:761771110
2029 2029 c, t dbSNP:111795945
2037 2037 a, g dbSNP:199926163
2046 2046 a, c dbSNP:773228076
2050 2050 a, g dbSNP:762991238
2051 2051 a, t dbSNP:765943808
2057 2057 c, g dbSNP:751100162
2066 2066 a, g dbSNP:759151717
2076 2076 c, t dbSNP:767062466
2103 2103 c, t dbSNP:775066227
2104 2104 c, g dbSNP:760324497
2105 2105 a, c, t dbSNP:116097791
2106 2106 a, g dbSNP:114545628
2115 2115 c, t dbSNP:765840442
2117 2117 c, t dbSNP:141127966
2118 2118 a, g dbSNP:758448736
2121 2121 c, t dbSNP:780006014
2125 2125 a, g dbSNP:747198406
2128 2128 a, c dbSNP:145243180
2130 2130 a, g dbSNP:755269798
2132 2132 a, g dbSNP:777684375
2134 2134 a, g dbSNP:749133071
2140 2140 c, g, t dbSNP:370200905
2141 2141 a, g dbSNP:774403163
2153 2153 c, g dbSNP:745804589
2157 2157 a, g dbSNP:375665108
2158 2158 a, c dbSNP:775011277
2162 2162 c, t dbSNP:760299925
2167 2167 a, g dbSNP:368690834
2177 2177 c, t dbSNP:776977900
2178 2178 a, g dbSNP:116628504
2179 2179 g, t dbSNP:765535877
2194 2194 c, t dbSNP:368254411
2196 2196 a, g dbSNP:138105397
2198 2198 a, c dbSNP:766430404
2215 2215 a, g dbSNP:767717703
2218 2218 a, g, t dbSNP:752896534
2226 2226 a, g dbSNP:778719316
2257 2257 c, t dbSNP:750226582
2263 2263 c, g dbSNP:758352141
2269 2269 c, t dbSNP:780026522
2272 2272 c, t dbSNP:758454149
2273 2273 c, t dbSNP:550322570
2289 2289 a, t dbSNP:768089988
2290 2290 c, t dbSNP:3793801
2299 2299 c, t dbSNP:747791343
2304 2304 c, g dbSNP:201439531
2306 2306 a, g dbSNP:769377082
2307 2307 c, g dbSNP:773591109
2328 2328 -, ctt dbSNP:750942168
2350 2350 a, c dbSNP:145995632
2356 2356 a, g dbSNP:747261452
2362 2362 a, c dbSNP:771548584
2364 2364 a, g dbSNP:535850567
2365 2365 a, c dbSNP:114208185
2368 2368 c, t dbSNP:760123165
2392 2392 c, t dbSNP:767664583
2393 2393 a, c dbSNP:752740939
2394 2394 g, t dbSNP:760920908
2395 2395 a, g dbSNP:566628025
2400 2400 c, g, t dbSNP:79414242
2401 2401 a, g dbSNP:77144546
2406 2406 c, t dbSNP:74334349
2408 2408 a, g dbSNP:751461280
2410 2410 a, g dbSNP:754935493
2428 2428 -, a dbSNP:768461054
2432 2432 a, g dbSNP:780662703
2435 2435 a, g dbSNP:142285373
2442 2442 c, t dbSNP:755783618
2445 2445 a, g dbSNP:114719193
2449 2449 c, g dbSNP:748981265
2454 2454 a, g dbSNP:771354440
2465 2465 c, g dbSNP:774886016
2466 2466 a, c dbSNP:746327923
2472 2472 c, t dbSNP:772755011
2475 2475 c, t dbSNP:374303993
2479 2479 a, g dbSNP:775635830
2484 2484 g, t dbSNP:760725539
2488 2488 a, c dbSNP:764248770
2489 2489 a, c dbSNP:776653200
2490 2490 c, t dbSNP:762055920
2491 2491 a, c dbSNP:766117753
2492 2492 c, t dbSNP:751408195
2493 2493 c, g dbSNP:578155039
2495 2495 a, c dbSNP:754875240
2498 2498 -, c dbSNP:145178233
2498 2498 a, c dbSNP:767416437
2500 2500 c, g dbSNP:759489601
2501 2501 c, t dbSNP:752234656
2504 2504 a, c dbSNP:199684404
2514 2514 -, agcca dbSNP:780315825
2522 2522 c, t dbSNP:755519156

Target ORF information:

RefSeq Version NM_001142934
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant S, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001142934

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16386
Accession Version NM_020986.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D82340.1, D82341.1, AC073366.4, BC130615.1 and AF305907.1. On May 21, 2008 this sequence version replaced gi:119433670. Summary: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]. Transcript Variant: This variant (N2) contains alternate 5' exon N and encodes isoform 1 (PMID 9073174). Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF305909.1, BC130615.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_066266.3
CDS164..2056
Misc Feature(1)11..13(+)
Misc Feature(2)200..1969(+)
Exon (1)1..95
Gene:CHAT
Gene Synonym:
Exon (2)96..196
Gene:CHAT
Gene Synonym:
Exon (3)197..388
Gene:CHAT
Gene Synonym:
Exon (4)389..507
Gene:CHAT
Gene Synonym:
Exon (5)508..561
Gene:CHAT
Gene Synonym:
Exon (6)562..742
Gene:CHAT
Gene Synonym:
Exon (7)743..920
Gene:CHAT
Gene Synonym:
Exon (8)921..1090
Gene:CHAT
Gene Synonym:
Exon (9)1091..1191
Gene:CHAT
Gene Synonym:
Exon (10)1192..1320
Gene:CHAT
Gene Synonym:
Exon (11)1321..1443
Gene:CHAT
Gene Synonym:
Exon (12)1444..1585
Gene:CHAT
Gene Synonym:
Exon (13)1586..1648
Gene:CHAT
Gene Synonym:
Exon (14)1649..1786
Gene:CHAT
Gene Synonym:
Exon (15)1787..2114
Gene:CHAT
Gene Synonym:
Translation
Position Chain Variation Link
9 9 g, t dbSNP:77723675
29 29 a, g dbSNP:751757192
38 38 g, t dbSNP:3729496
45 45 c, t dbSNP:553208755
65 65 a, g dbSNP:571385948
66 66 c, t dbSNP:538800349
90 90 a, g dbSNP:3810947
105 105 c, t dbSNP:746229284
123 123 c, g dbSNP:149711635
125 125 c, t dbSNP:776042082
135 135 c, t dbSNP:542610160
136 136 a, g dbSNP:79914771
158 158 c, g, t dbSNP:200361897
159 159 a, g, t dbSNP:376641216
166 166 a, g dbSNP:759310217
167 167 a, g dbSNP:3810950
171 171 c, t dbSNP:752726003
183 183 a, g dbSNP:756138129
188 188 a, g dbSNP:777374223
191 191 a, g dbSNP:753288753
194 194 a, t dbSNP:756873370
196 196 c, t dbSNP:143723043
197 197 g, t dbSNP:781596921
200 200 c, t dbSNP:748225279
202 202 g, t dbSNP:769827861
214 214 a, c, g, t dbSNP:757344796
215 215 a, g dbSNP:201479289
217 217 a, g dbSNP:199527987
218 218 c, t dbSNP:376188733
220 220 c, t dbSNP:764063564
222 222 c, g, t dbSNP:557059182
223 223 a, g dbSNP:199675090
226 226 c, g dbSNP:116390167
229 229 a, g dbSNP:758005278
231 231 a, c dbSNP:781476714
243 243 c, g, t dbSNP:766040346
244 244 a, g dbSNP:77296560
247 247 c, t dbSNP:61731734
250 250 a, g dbSNP:753198768
251 251 a, c dbSNP:555796797
260 260 a, c dbSNP:777679246
261 261 a, g dbSNP:202059535
271 271 a, g dbSNP:771258646
283 283 a, g dbSNP:143662656
301 301 c, t dbSNP:746798016
303 303 c, t dbSNP:148155075
315 315 c, t dbSNP:776499844
318 318 a, g dbSNP:370097299
323 323 c, t dbSNP:769800832
325 325 c, t dbSNP:772659997
329 329 a, g dbSNP:762497836
331 331 a, c, t dbSNP:765982984
332 332 c, t dbSNP:759928572
333 333 c, t dbSNP:767712700
334 334 c, t dbSNP:753106995
335 335 a, g dbSNP:374586812
337 337 c, t dbSNP:778476300
338 338 a, g dbSNP:115032198
341 341 a, t dbSNP:757480104
349 349 c, g dbSNP:200143133
356 356 a, c dbSNP:746030420
363 363 a, t dbSNP:757287480
365 365 c, t dbSNP:141111059
366 366 a, c, g dbSNP:747996335
369 369 -, agg dbSNP:758703555
372 372 a, g dbSNP:75011234
385 385 c, t dbSNP:150236872
403 403 c, g dbSNP:773806458
411 411 a, g dbSNP:745437517
414 414 g, t dbSNP:376808313
427 427 a, c dbSNP:775282060
428 428 c, t dbSNP:760936252
429 429 a, c, g dbSNP:764497513
430 430 c, t dbSNP:367783660
431 431 c, t dbSNP:377304752
438 438 c, t dbSNP:121912820
439 439 a, g dbSNP:750447540
440 440 c, g dbSNP:121912815
451 451 c, t dbSNP:758544604
460 460 c, g, t dbSNP:575515952
461 461 a, g dbSNP:751863010
469 469 c, t dbSNP:755203528
470 470 a, g dbSNP:778708569
474 474 a, c, g dbSNP:8178989
484 484 c, t dbSNP:267602510
487 487 c, t dbSNP:142740451
488 488 a, g dbSNP:778954064
491 491 a, t dbSNP:745367841
493 493 c, t dbSNP:79030468
494 494 a, g dbSNP:774877358
497 497 c, g dbSNP:746608168
512 512 a, g dbSNP:748516964
516 516 c, t dbSNP:770250348
520 520 c, g dbSNP:78925077
521 521 c, g dbSNP:375606885
533 533 a, g dbSNP:137899400
536 536 c, t dbSNP:8178990
546 546 a, g dbSNP:774436099
549 549 c, t dbSNP:561562136
550 550 c, g dbSNP:767727254
553 553 c, g dbSNP:752976973
554 554 c, g dbSNP:115510708
556 556 a, g dbSNP:765018013
563 563 c, t dbSNP:376994468
576 576 c, t dbSNP:754977595
592 592 c, t dbSNP:767065663
596 596 c, t dbSNP:74133815
598 598 a, c, g dbSNP:114090981
603 603 a, g dbSNP:777336258
607 607 a, g dbSNP:142237923
609 609 c, t dbSNP:749662741
615 615 g, t dbSNP:757624145
621 621 a, g dbSNP:779266134
629 629 g, t dbSNP:746310436
637 637 c, g dbSNP:772600687
640 640 -, ctc dbSNP:560648873
643 643 c, t dbSNP:775502083
650 650 c, t dbSNP:747108035
652 652 c, g dbSNP:768892260
659 659 a, g dbSNP:146236256
667 667 c, t dbSNP:200423662
673 673 c, t dbSNP:766217426
675 675 c, t dbSNP:139352363
676 676 a, g, t dbSNP:574542737
677 677 c, t dbSNP:752229226
695 695 a, g dbSNP:141599748
704 704 c, t dbSNP:540899575
705 705 c, t dbSNP:868749
706 706 a, g dbSNP:756942813
712 712 c, t dbSNP:113897064
713 713 c, g dbSNP:746255012
718 718 c, g, t dbSNP:76570508
719 719 a, g dbSNP:116362950
722 722 a, g dbSNP:370481031
723 723 c, g, t dbSNP:75466054
725 725 a, g dbSNP:551219437
727 727 -, agcc dbSNP:769980623
728 728 a, g dbSNP:776785129
729 729 c, t dbSNP:748250869
738 738 a, g dbSNP:149976821
759 759 c, t dbSNP:778178080
773 773 c, t dbSNP:147815999
776 776 c, t dbSNP:771938918
777 777 a, c, g dbSNP:200176236
783 783 c, g dbSNP:768665020
789 789 a, g dbSNP:546158744
790 790 a, g, t dbSNP:201359694
791 791 a, g dbSNP:370202834
793 793 c, t dbSNP:750133075
804 804 a, g dbSNP:762605295
815 815 a, t dbSNP:766585447
816 816 c, t dbSNP:121912823
825 825 c, t dbSNP:375439849
834 834 a, g dbSNP:781745178
835 835 c, t dbSNP:752685760
836 836 a, g dbSNP:778837182
841 841 c, t dbSNP:145203976
842 842 a, g dbSNP:200776329
845 845 c, t dbSNP:182935725
846 846 a, g dbSNP:779668516
847 847 c, t dbSNP:367961073
853 853 a, t dbSNP:200009054
854 854 c, g dbSNP:776699190
859 859 a, t dbSNP:74340719
868 868 a, g dbSNP:747645217
870 870 c, t dbSNP:769234940
871 871 a, g dbSNP:529337162
877 877 c, t dbSNP:748193892
878 878 a, g dbSNP:61731735
883 883 a, g dbSNP:371905802
886 886 c, t dbSNP:759981661
887 887 a, g dbSNP:767903393
892 892 a, g dbSNP:753310881
895 895 c, g, t dbSNP:374870408
896 896 a, g dbSNP:75262191
898 898 a, g dbSNP:757402644
900 900 c, t dbSNP:779237093
906 906 a, c, t dbSNP:533289708
907 907 a, g dbSNP:780860401
913 913 c, t dbSNP:551349781
914 914 a, g, t dbSNP:769754418
931 931 c, t dbSNP:61115650
932 932 a, c, t dbSNP:141794970
933 933 a, g dbSNP:201616704
940 940 a, g dbSNP:115126024
944 944 c, g dbSNP:115212829
948 948 c, t dbSNP:778441042
951 951 c, t dbSNP:372758559
956 956 c, t dbSNP:771822812
957 957 a, g dbSNP:779919147
970 970 c, t dbSNP:562306374
971 971 c, g dbSNP:747366078
974 974 c, t dbSNP:769085306
976 976 c, t dbSNP:777142490
982 982 c, t dbSNP:141690626
983 983 a, g dbSNP:115877658
984 984 c, t dbSNP:114932900
985 985 a, g dbSNP:200321426
986 986 c, t dbSNP:201553605
995 995 a, g dbSNP:536359684
1006 1006 c, t dbSNP:554276268
1007 1007 a, g dbSNP:8178991
1011 1011 a, c dbSNP:539884711
1020 1020 a, c, t dbSNP:192592698
1036 1036 c, t dbSNP:578201320
1037 1037 a, g, t dbSNP:749948399
1039 1039 c, t dbSNP:779724822
1040 1040 a, g, t dbSNP:746595362
1043 1043 a, g dbSNP:781569427
1045 1045 a, c dbSNP:748510157
1048 1048 c, t dbSNP:770303527
1057 1057 c, t dbSNP:116071049
1062 1062 c, t dbSNP:376230019
1067 1067 c, t dbSNP:121912822
1069 1069 c, t dbSNP:770937861
1075 1075 c, t dbSNP:201381950
1076 1076 a, g dbSNP:759707536
1084 1084 a, c dbSNP:767778694
1092 1092 c, t dbSNP:774394630
1097 1097 a, g dbSNP:745991038
1104 1104 a, g dbSNP:377494035
1108 1108 c, t dbSNP:146284420
1109 1109 a, g dbSNP:115964813
1116 1116 a, g dbSNP:139416405
1117 1117 c, t dbSNP:764875843
1118 1118 a, g dbSNP:562560467
1128 1128 a, g dbSNP:762936544
1129 1129 c, t dbSNP:766466820
1130 1130 a, g dbSNP:121912816
1132 1132 a, g dbSNP:759183550
1137 1137 -, c dbSNP:768533400
1139 1139 c, g dbSNP:767180792
1144 1144 a, c, t dbSNP:752229820
1147 1147 c, t dbSNP:777957584
1153 1153 c, t dbSNP:754253168
1154 1154 a, g dbSNP:757733319
1160 1160 c, g dbSNP:779466347
1169 1169 a, g dbSNP:745938985
1181 1181 c, t dbSNP:76014951
1184 1184 a, c, g dbSNP:769398642
1190 1190 a, g dbSNP:4838544
1192 1192 a, g dbSNP:370918939
1193 1193 a, g dbSNP:781625888
1194 1194 c, t dbSNP:538478136
1195 1195 a, g dbSNP:770792319
1200 1200 a, g dbSNP:148525631
1217 1217 a, c, t dbSNP:142889639
1218 1218 a, g dbSNP:774834313
1219 1219 a, c dbSNP:760300737
1228 1228 c, t dbSNP:200175587
1229 1229 a, g dbSNP:371719364
1230 1230 c, t dbSNP:761462857
1232 1232 a, t dbSNP:765549293
1234 1234 a, c, t dbSNP:553771451
1235 1235 a, g dbSNP:766947649
1244 1244 -, c dbSNP:773268576
1244 1244 a, g dbSNP:3793795
1250 1250 c, g, t dbSNP:751583653
1251 1251 a, g dbSNP:202125860
1252 1252 a, g dbSNP:748314389
1253 1253 a, c, g dbSNP:121912818
1259 1259 a, c, t dbSNP:778801148
1260 1260 a, g dbSNP:771901229
1261 1261 a, c, g dbSNP:775557792
1263 1263 a, g dbSNP:768087857
1268 1268 a, t dbSNP:375424189
1269 1269 g, t dbSNP:201293521
1275 1275 c, t dbSNP:764921945
1276 1276 a, g dbSNP:558057516
1277 1277 -, ga dbSNP:760039781
1279 1279 a, c dbSNP:773420869
1284 1284 a, c dbSNP:763439208
1294 1294 a, g dbSNP:151031033
1299 1299 c, t dbSNP:141878259
1302 1302 c, t dbSNP:121912821
1303 1303 a, g dbSNP:139722660
1304 1304 a, g dbSNP:767664496
1306 1306 a, g dbSNP:752844751
1312 1312 a, c, t dbSNP:756381050
1319 1319 c, t dbSNP:371470622
1320 1320 a, g dbSNP:200335347
1325 1325 g, t dbSNP:121912817
1337 1337 g, t dbSNP:752482024
1339 1339 c, t dbSNP:558298529
1344 1344 c, t dbSNP:769232976
1348 1348 a, c dbSNP:777148519
1350 1350 a, g dbSNP:144918120
1373 1373 a, g dbSNP:770511614
1375 1375 a, c, g dbSNP:74433353
1376 1376 c, t dbSNP:760016941
1379 1379 a, g dbSNP:75981983
1414 1414 c, g dbSNP:772695708
1417 1417 a, g dbSNP:758135155
1426 1426 c, g dbSNP:776084200
1444 1444 g, t dbSNP:201857639
1450 1450 a, c, t dbSNP:8178992
1451 1451 c, t dbSNP:369251527
1452 1452 a, g dbSNP:141881680
1455 1455 g, t dbSNP:756056022
1456 1456 a, g dbSNP:763978618
1463 1463 c, t dbSNP:147079245
1466 1466 a, c dbSNP:756665776
1470 1470 a, g dbSNP:778572568
1471 1471 c, t dbSNP:145370753
1472 1472 a, g dbSNP:757303526
1477 1477 c, t dbSNP:55702495
1478 1478 a, g dbSNP:372760913
1479 1479 c, t dbSNP:769204680
1480 1480 a, g dbSNP:777066390
1483 1483 c, t dbSNP:7073028
1484 1484 a, g dbSNP:202040717
1487 1487 c, t dbSNP:773307141
1488 1488 a, g dbSNP:121912819
1491 1491 a, g dbSNP:80097077
1505 1505 c, t dbSNP:368957556
1507 1507 c, t dbSNP:760452753
1508 1508 a, g dbSNP:764077878
1512 1512 -, aca dbSNP:139324199
1524 1524 c, g dbSNP:753652169
1525 1525 a, g dbSNP:374000183
1541 1541 c, t dbSNP:764791712
1544 1544 a, g dbSNP:750002983
1554 1554 c, g dbSNP:758095810
1555 1555 a, g dbSNP:779923095
1558 1558 c, g, t dbSNP:201580702
1559 1559 a, g dbSNP:547073881
1576 1576 c, t dbSNP:781621361
1577 1577 g, t dbSNP:376947247
1580 1580 a, g dbSNP:201485243
1593 1593 a, t dbSNP:190968891
1609 1609 a, g, t dbSNP:373259575
1611 1611 a, c dbSNP:776260066
1614 1614 a, g dbSNP:761771110
1615 1615 c, t dbSNP:111795945
1623 1623 a, g dbSNP:199926163
1632 1632 a, c dbSNP:773228076
1636 1636 a, g dbSNP:762991238
1637 1637 a, t dbSNP:765943808
1643 1643 c, g dbSNP:751100162
1652 1652 a, g dbSNP:759151717
1662 1662 c, t dbSNP:767062466
1689 1689 c, t dbSNP:775066227
1690 1690 c, g dbSNP:760324497
1691 1691 a, c, t dbSNP:116097791
1692 1692 a, g dbSNP:114545628
1701 1701 c, t dbSNP:765840442
1703 1703 c, t dbSNP:141127966
1704 1704 a, g dbSNP:758448736
1707 1707 c, t dbSNP:780006014
1711 1711 a, g dbSNP:747198406
1714 1714 a, c dbSNP:145243180
1716 1716 a, g dbSNP:755269798
1718 1718 a, g dbSNP:777684375
1720 1720 a, g dbSNP:749133071
1726 1726 c, g, t dbSNP:370200905
1727 1727 a, g dbSNP:774403163
1739 1739 c, g dbSNP:745804589
1743 1743 a, g dbSNP:375665108
1744 1744 a, c dbSNP:775011277
1748 1748 c, t dbSNP:760299925
1753 1753 a, g dbSNP:368690834
1763 1763 c, t dbSNP:776977900
1764 1764 a, g dbSNP:116628504
1765 1765 g, t dbSNP:765535877
1780 1780 c, t dbSNP:368254411
1782 1782 a, g dbSNP:138105397
1784 1784 a, c dbSNP:766430404
1801 1801 a, g dbSNP:767717703
1804 1804 a, g, t dbSNP:752896534
1812 1812 a, g dbSNP:778719316
1843 1843 c, t dbSNP:750226582
1849 1849 c, g dbSNP:758352141
1855 1855 c, t dbSNP:780026522
1858 1858 c, t dbSNP:758454149
1859 1859 c, t dbSNP:550322570
1875 1875 a, t dbSNP:768089988
1876 1876 c, t dbSNP:3793801
1885 1885 c, t dbSNP:747791343
1890 1890 c, g dbSNP:201439531
1892 1892 a, g dbSNP:769377082
1893 1893 c, g dbSNP:773591109
1914 1914 -, ctt dbSNP:750942168
1936 1936 a, c dbSNP:145995632
1942 1942 a, g dbSNP:747261452
1948 1948 a, c dbSNP:771548584
1950 1950 a, g dbSNP:535850567
1951 1951 a, c dbSNP:114208185
1954 1954 c, t dbSNP:760123165
1978 1978 c, t dbSNP:767664583
1979 1979 a, c dbSNP:752740939
1980 1980 g, t dbSNP:760920908
1981 1981 a, g dbSNP:566628025
1986 1986 c, g, t dbSNP:79414242
1987 1987 a, g dbSNP:77144546
1992 1992 c, t dbSNP:74334349
1994 1994 a, g dbSNP:751461280
1996 1996 a, g dbSNP:754935493
2014 2014 -, a dbSNP:768461054
2018 2018 a, g dbSNP:780662703
2021 2021 a, g dbSNP:142285373
2028 2028 c, t dbSNP:755783618
2031 2031 a, g dbSNP:114719193
2035 2035 c, g dbSNP:748981265
2040 2040 a, g dbSNP:771354440
2051 2051 c, g dbSNP:774886016
2052 2052 a, c dbSNP:746327923
2058 2058 c, t dbSNP:772755011
2061 2061 c, t dbSNP:374303993
2065 2065 a, g dbSNP:775635830
2070 2070 g, t dbSNP:760725539
2074 2074 a, c dbSNP:764248770
2075 2075 a, c dbSNP:776653200
2076 2076 c, t dbSNP:762055920
2077 2077 a, c dbSNP:766117753
2078 2078 c, t dbSNP:751408195
2079 2079 c, g dbSNP:578155039
2081 2081 a, c dbSNP:754875240
2084 2084 -, c dbSNP:145178233
2084 2084 a, c dbSNP:767416437
2086 2086 c, g dbSNP:759489601
2087 2087 c, t dbSNP:752234656
2090 2090 a, c dbSNP:199684404
2100 2100 -, agcca dbSNP:780315825
2108 2108 c, t dbSNP:755519156

Target ORF information:

RefSeq Version NM_020986
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant N2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020986

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16386
Accession Version NM_001142929.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073366.4 and AF305907.1. Summary: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]. Transcript Variant: This variant (M) contains alternate 5' exon M (PMID 7616604) but uses a downstream start codon and encodes isoform 1. Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF305907.1, S56138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1337937) ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001136401.1
CDS508..2400
Misc Feature(1)7..9(+)
Misc Feature(2)544..2313(+)
Exon (1)1..439
Gene:CHAT
Gene Synonym:
Exon (2)440..540
Gene:CHAT
Gene Synonym:
Exon (3)541..732
Gene:CHAT
Gene Synonym:
Exon (4)733..851
Gene:CHAT
Gene Synonym:
Exon (5)852..905
Gene:CHAT
Gene Synonym:
Exon (6)906..1086
Gene:CHAT
Gene Synonym:
Exon (7)1087..1264
Gene:CHAT
Gene Synonym:
Exon (8)1265..1434
Gene:CHAT
Gene Synonym:
Exon (9)1435..1535
Gene:CHAT
Gene Synonym:
Exon (10)1536..1664
Gene:CHAT
Gene Synonym:
Exon (11)1665..1787
Gene:CHAT
Gene Synonym:
Exon (12)1788..1929
Gene:CHAT
Gene Synonym:
Exon (13)1930..1992
Gene:CHAT
Gene Synonym:
Exon (14)1993..2130
Gene:CHAT
Gene Synonym:
Exon (15)2131..2458
Gene:CHAT
Gene Synonym:
Translation
Position Chain Variation Link
16 16 a, g dbSNP:764161559
51 51 c, g dbSNP:563585126
79 79 a, g dbSNP:755878487
110 110 a, c, g dbSNP:7903315
129 129 c, t dbSNP:751007736
134 134 g, t dbSNP:758531527
137 137 a, g dbSNP:77152496
144 144 g, t dbSNP:7923716
148 148 g, t dbSNP:755091583
168 168 a, c dbSNP:781493867
169 169 a, g dbSNP:749073862
174 174 g, t dbSNP:79528320
178 178 a, g dbSNP:587780314
183 183 c, g dbSNP:528479799
188 188 g, t dbSNP:770749789
190 190 a, g dbSNP:750228985
192 192 a, g dbSNP:12413661
198 198 a, g dbSNP:778795905
206 206 -, ag dbSNP:531450737
209 209 c, g dbSNP:546932446
230 230 c, g dbSNP:374430783
237 237 g, t dbSNP:745753124
238 238 a, c dbSNP:772025588
248 248 a, g dbSNP:377156965
267 267 c, g dbSNP:746540866
269 269 a, c dbSNP:370609858
270 270 c, g dbSNP:768179285
272 272 g, t dbSNP:776411377
294 294 c, g dbSNP:3810948
301 301 g, t dbSNP:765746009
306 306 a, t dbSNP:773735383
309 309 -, a dbSNP:745552600
327 327 a, c dbSNP:572002174
329 329 c, t dbSNP:766916844
340 340 g, t dbSNP:751539916
362 362 c, t dbSNP:755147781
375 375 c, t dbSNP:767600029
380 380 c, t dbSNP:752962530
386 386 a, c dbSNP:550874261
391 391 c, g dbSNP:756348058
405 405 a, t dbSNP:112079336
409 409 -, cggc dbSNP:761691250
411 411 c, g dbSNP:778636468
415 415 a, g dbSNP:372018292
428 428 c, t dbSNP:372298555
429 429 a, g dbSNP:745665140
449 449 c, t dbSNP:746229284
467 467 c, g dbSNP:149711635
469 469 c, t dbSNP:776042082
479 479 c, t dbSNP:542610160
480 480 a, g dbSNP:79914771
502 502 c, g, t dbSNP:200361897
503 503 a, g, t dbSNP:376641216
510 510 a, g dbSNP:759310217
511 511 a, g dbSNP:3810950
515 515 c, t dbSNP:752726003
527 527 a, g dbSNP:756138129
532 532 a, g dbSNP:777374223
535 535 a, g dbSNP:753288753
538 538 a, t dbSNP:756873370
540 540 c, t dbSNP:143723043
541 541 g, t dbSNP:781596921
544 544 c, t dbSNP:748225279
546 546 g, t dbSNP:769827861
558 558 a, c, g, t dbSNP:757344796
559 559 a, g dbSNP:201479289
561 561 a, g dbSNP:199527987
562 562 c, t dbSNP:376188733
564 564 c, t dbSNP:764063564
566 566 c, g, t dbSNP:557059182
567 567 a, g dbSNP:199675090
570 570 c, g dbSNP:116390167
573 573 a, g dbSNP:758005278
575 575 a, c dbSNP:781476714
587 587 c, g, t dbSNP:766040346
588 588 a, g dbSNP:77296560
591 591 c, t dbSNP:61731734
594 594 a, g dbSNP:753198768
595 595 a, c dbSNP:555796797
604 604 a, c dbSNP:777679246
605 605 a, g dbSNP:202059535
615 615 a, g dbSNP:771258646
627 627 a, g dbSNP:143662656
645 645 c, t dbSNP:746798016
647 647 c, t dbSNP:148155075
659 659 c, t dbSNP:776499844
662 662 a, g dbSNP:370097299
667 667 c, t dbSNP:769800832
669 669 c, t dbSNP:772659997
673 673 a, g dbSNP:762497836
675 675 a, c, t dbSNP:765982984
676 676 c, t dbSNP:759928572
677 677 c, t dbSNP:767712700
678 678 c, t dbSNP:753106995
679 679 a, g dbSNP:374586812
681 681 c, t dbSNP:778476300
682 682 a, g dbSNP:115032198
685 685 a, t dbSNP:757480104
693 693 c, g dbSNP:200143133
700 700 a, c dbSNP:746030420
707 707 a, t dbSNP:757287480
709 709 c, t dbSNP:141111059
710 710 a, c, g dbSNP:747996335
713 713 -, agg dbSNP:758703555
716 716 a, g dbSNP:75011234
729 729 c, t dbSNP:150236872
747 747 c, g dbSNP:773806458
755 755 a, g dbSNP:745437517
758 758 g, t dbSNP:376808313
771 771 a, c dbSNP:775282060
772 772 c, t dbSNP:760936252
773 773 a, c, g dbSNP:764497513
774 774 c, t dbSNP:367783660
775 775 c, t dbSNP:377304752
782 782 c, t dbSNP:121912820
783 783 a, g dbSNP:750447540
784 784 c, g dbSNP:121912815
795 795 c, t dbSNP:758544604
804 804 c, g, t dbSNP:575515952
805 805 a, g dbSNP:751863010
813 813 c, t dbSNP:755203528
814 814 a, g dbSNP:778708569
818 818 a, c, g dbSNP:8178989
828 828 c, t dbSNP:267602510
831 831 c, t dbSNP:142740451
832 832 a, g dbSNP:778954064
835 835 a, t dbSNP:745367841
837 837 c, t dbSNP:79030468
838 838 a, g dbSNP:774877358
841 841 c, g dbSNP:746608168
856 856 a, g dbSNP:748516964
860 860 c, t dbSNP:770250348
864 864 c, g dbSNP:78925077
865 865 c, g dbSNP:375606885
877 877 a, g dbSNP:137899400
880 880 c, t dbSNP:8178990
890 890 a, g dbSNP:774436099
893 893 c, t dbSNP:561562136
894 894 c, g dbSNP:767727254
897 897 c, g dbSNP:752976973
898 898 c, g dbSNP:115510708
900 900 a, g dbSNP:765018013
907 907 c, t dbSNP:376994468
920 920 c, t dbSNP:754977595
936 936 c, t dbSNP:767065663
940 940 c, t dbSNP:74133815
942 942 a, c, g dbSNP:114090981
947 947 a, g dbSNP:777336258
951 951 a, g dbSNP:142237923
953 953 c, t dbSNP:749662741
959 959 g, t dbSNP:757624145
965 965 a, g dbSNP:779266134
973 973 g, t dbSNP:746310436
981 981 c, g dbSNP:772600687
984 984 -, ctc dbSNP:560648873
987 987 c, t dbSNP:775502083
994 994 c, t dbSNP:747108035
996 996 c, g dbSNP:768892260
1003 1003 a, g dbSNP:146236256
1011 1011 c, t dbSNP:200423662
1017 1017 c, t dbSNP:766217426
1019 1019 c, t dbSNP:139352363
1020 1020 a, g, t dbSNP:574542737
1021 1021 c, t dbSNP:752229226
1039 1039 a, g dbSNP:141599748
1048 1048 c, t dbSNP:540899575
1049 1049 c, t dbSNP:868749
1050 1050 a, g dbSNP:756942813
1056 1056 c, t dbSNP:113897064
1057 1057 c, g dbSNP:746255012
1062 1062 c, g, t dbSNP:76570508
1063 1063 a, g dbSNP:116362950
1066 1066 a, g dbSNP:370481031
1067 1067 c, g, t dbSNP:75466054
1069 1069 a, g dbSNP:551219437
1071 1071 -, agcc dbSNP:769980623
1072 1072 a, g dbSNP:776785129
1073 1073 c, t dbSNP:748250869
1082 1082 a, g dbSNP:149976821
1103 1103 c, t dbSNP:778178080
1117 1117 c, t dbSNP:147815999
1120 1120 c, t dbSNP:771938918
1121 1121 a, c, g dbSNP:200176236
1127 1127 c, g dbSNP:768665020
1133 1133 a, g dbSNP:546158744
1134 1134 a, g, t dbSNP:201359694
1135 1135 a, g dbSNP:370202834
1137 1137 c, t dbSNP:750133075
1148 1148 a, g dbSNP:762605295
1159 1159 a, t dbSNP:766585447
1160 1160 c, t dbSNP:121912823
1169 1169 c, t dbSNP:375439849
1178 1178 a, g dbSNP:781745178
1179 1179 c, t dbSNP:752685760
1180 1180 a, g dbSNP:778837182
1185 1185 c, t dbSNP:145203976
1186 1186 a, g dbSNP:200776329
1189 1189 c, t dbSNP:182935725
1190 1190 a, g dbSNP:779668516
1191 1191 c, t dbSNP:367961073
1197 1197 a, t dbSNP:200009054
1198 1198 c, g dbSNP:776699190
1203 1203 a, t dbSNP:74340719
1212 1212 a, g dbSNP:747645217
1214 1214 c, t dbSNP:769234940
1215 1215 a, g dbSNP:529337162
1221 1221 c, t dbSNP:748193892
1222 1222 a, g dbSNP:61731735
1227 1227 a, g dbSNP:371905802
1230 1230 c, t dbSNP:759981661
1231 1231 a, g dbSNP:767903393
1236 1236 a, g dbSNP:753310881
1239 1239 c, g, t dbSNP:374870408
1240 1240 a, g dbSNP:75262191
1242 1242 a, g dbSNP:757402644
1244 1244 c, t dbSNP:779237093
1250 1250 a, c, t dbSNP:533289708
1251 1251 a, g dbSNP:780860401
1257 1257 c, t dbSNP:551349781
1258 1258 a, g, t dbSNP:769754418
1275 1275 c, t dbSNP:61115650
1276 1276 a, c, t dbSNP:141794970
1277 1277 a, g dbSNP:201616704
1284 1284 a, g dbSNP:115126024
1288 1288 c, g dbSNP:115212829
1292 1292 c, t dbSNP:778441042
1295 1295 c, t dbSNP:372758559
1300 1300 c, t dbSNP:771822812
1301 1301 a, g dbSNP:779919147
1314 1314 c, t dbSNP:562306374
1315 1315 c, g dbSNP:747366078
1318 1318 c, t dbSNP:769085306
1320 1320 c, t dbSNP:777142490
1326 1326 c, t dbSNP:141690626
1327 1327 a, g dbSNP:115877658
1328 1328 c, t dbSNP:114932900
1329 1329 a, g dbSNP:200321426
1330 1330 c, t dbSNP:201553605
1339 1339 a, g dbSNP:536359684
1350 1350 c, t dbSNP:554276268
1351 1351 a, g dbSNP:8178991
1355 1355 a, c dbSNP:539884711
1364 1364 a, c, t dbSNP:192592698
1380 1380 c, t dbSNP:578201320
1381 1381 a, g, t dbSNP:749948399
1383 1383 c, t dbSNP:779724822
1384 1384 a, g, t dbSNP:746595362
1387 1387 a, g dbSNP:781569427
1389 1389 a, c dbSNP:748510157
1392 1392 c, t dbSNP:770303527
1401 1401 c, t dbSNP:116071049
1406 1406 c, t dbSNP:376230019
1411 1411 c, t dbSNP:121912822
1413 1413 c, t dbSNP:770937861
1419 1419 c, t dbSNP:201381950
1420 1420 a, g dbSNP:759707536
1428 1428 a, c dbSNP:767778694
1436 1436 c, t dbSNP:774394630
1441 1441 a, g dbSNP:745991038
1448 1448 a, g dbSNP:377494035
1452 1452 c, t dbSNP:146284420
1453 1453 a, g dbSNP:115964813
1460 1460 a, g dbSNP:139416405
1461 1461 c, t dbSNP:764875843
1462 1462 a, g dbSNP:562560467
1472 1472 a, g dbSNP:762936544
1473 1473 c, t dbSNP:766466820
1474 1474 a, g dbSNP:121912816
1476 1476 a, g dbSNP:759183550
1481 1481 -, c dbSNP:768533400
1483 1483 c, g dbSNP:767180792
1488 1488 a, c, t dbSNP:752229820
1491 1491 c, t dbSNP:777957584
1497 1497 c, t dbSNP:754253168
1498 1498 a, g dbSNP:757733319
1504 1504 c, g dbSNP:779466347
1513 1513 a, g dbSNP:745938985
1525 1525 c, t dbSNP:76014951
1528 1528 a, c, g dbSNP:769398642
1534 1534 a, g dbSNP:4838544
1536 1536 a, g dbSNP:370918939
1537 1537 a, g dbSNP:781625888
1538 1538 c, t dbSNP:538478136
1539 1539 a, g dbSNP:770792319
1544 1544 a, g dbSNP:148525631
1561 1561 a, c, t dbSNP:142889639
1562 1562 a, g dbSNP:774834313
1563 1563 a, c dbSNP:760300737
1572 1572 c, t dbSNP:200175587
1573 1573 a, g dbSNP:371719364
1574 1574 c, t dbSNP:761462857
1576 1576 a, t dbSNP:765549293
1578 1578 a, c, t dbSNP:553771451
1579 1579 a, g dbSNP:766947649
1588 1588 -, c dbSNP:773268576
1588 1588 a, g dbSNP:3793795
1594 1594 c, g, t dbSNP:751583653
1595 1595 a, g dbSNP:202125860
1596 1596 a, g dbSNP:748314389
1597 1597 a, c, g dbSNP:121912818
1603 1603 a, c, t dbSNP:778801148
1604 1604 a, g dbSNP:771901229
1605 1605 a, c, g dbSNP:775557792
1607 1607 a, g dbSNP:768087857
1612 1612 a, t dbSNP:375424189
1613 1613 g, t dbSNP:201293521
1619 1619 c, t dbSNP:764921945
1620 1620 a, g dbSNP:558057516
1621 1621 -, ga dbSNP:760039781
1623 1623 a, c dbSNP:773420869
1628 1628 a, c dbSNP:763439208
1638 1638 a, g dbSNP:151031033
1643 1643 c, t dbSNP:141878259
1646 1646 c, t dbSNP:121912821
1647 1647 a, g dbSNP:139722660
1648 1648 a, g dbSNP:767664496
1650 1650 a, g dbSNP:752844751
1656 1656 a, c, t dbSNP:756381050
1663 1663 c, t dbSNP:371470622
1664 1664 a, g dbSNP:200335347
1669 1669 g, t dbSNP:121912817
1681 1681 g, t dbSNP:752482024
1683 1683 c, t dbSNP:558298529
1688 1688 c, t dbSNP:769232976
1692 1692 a, c dbSNP:777148519
1694 1694 a, g dbSNP:144918120
1717 1717 a, g dbSNP:770511614
1719 1719 a, c, g dbSNP:74433353
1720 1720 c, t dbSNP:760016941
1723 1723 a, g dbSNP:75981983
1758 1758 c, g dbSNP:772695708
1761 1761 a, g dbSNP:758135155
1770 1770 c, g dbSNP:776084200
1788 1788 g, t dbSNP:201857639
1794 1794 a, c, t dbSNP:8178992
1795 1795 c, t dbSNP:369251527
1796 1796 a, g dbSNP:141881680
1799 1799 g, t dbSNP:756056022
1800 1800 a, g dbSNP:763978618
1807 1807 c, t dbSNP:147079245
1810 1810 a, c dbSNP:756665776
1814 1814 a, g dbSNP:778572568
1815 1815 c, t dbSNP:145370753
1816 1816 a, g dbSNP:757303526
1821 1821 c, t dbSNP:55702495
1822 1822 a, g dbSNP:372760913
1823 1823 c, t dbSNP:769204680
1824 1824 a, g dbSNP:777066390
1827 1827 c, t dbSNP:7073028
1828 1828 a, g dbSNP:202040717
1831 1831 c, t dbSNP:773307141
1832 1832 a, g dbSNP:121912819
1835 1835 a, g dbSNP:80097077
1849 1849 c, t dbSNP:368957556
1851 1851 c, t dbSNP:760452753
1852 1852 a, g dbSNP:764077878
1856 1856 -, aca dbSNP:139324199
1868 1868 c, g dbSNP:753652169
1869 1869 a, g dbSNP:374000183
1885 1885 c, t dbSNP:764791712
1888 1888 a, g dbSNP:750002983
1898 1898 c, g dbSNP:758095810
1899 1899 a, g dbSNP:779923095
1902 1902 c, g, t dbSNP:201580702
1903 1903 a, g dbSNP:547073881
1920 1920 c, t dbSNP:781621361
1921 1921 g, t dbSNP:376947247
1924 1924 a, g dbSNP:201485243
1937 1937 a, t dbSNP:190968891
1953 1953 a, g, t dbSNP:373259575
1955 1955 a, c dbSNP:776260066
1958 1958 a, g dbSNP:761771110
1959 1959 c, t dbSNP:111795945
1967 1967 a, g dbSNP:199926163
1976 1976 a, c dbSNP:773228076
1980 1980 a, g dbSNP:762991238
1981 1981 a, t dbSNP:765943808
1987 1987 c, g dbSNP:751100162
1996 1996 a, g dbSNP:759151717
2006 2006 c, t dbSNP:767062466
2033 2033 c, t dbSNP:775066227
2034 2034 c, g dbSNP:760324497
2035 2035 a, c, t dbSNP:116097791
2036 2036 a, g dbSNP:114545628
2045 2045 c, t dbSNP:765840442
2047 2047 c, t dbSNP:141127966
2048 2048 a, g dbSNP:758448736
2051 2051 c, t dbSNP:780006014
2055 2055 a, g dbSNP:747198406
2058 2058 a, c dbSNP:145243180
2060 2060 a, g dbSNP:755269798
2062 2062 a, g dbSNP:777684375
2064 2064 a, g dbSNP:749133071
2070 2070 c, g, t dbSNP:370200905
2071 2071 a, g dbSNP:774403163
2083 2083 c, g dbSNP:745804589
2087 2087 a, g dbSNP:375665108
2088 2088 a, c dbSNP:775011277
2092 2092 c, t dbSNP:760299925
2097 2097 a, g dbSNP:368690834
2107 2107 c, t dbSNP:776977900
2108 2108 a, g dbSNP:116628504
2109 2109 g, t dbSNP:765535877
2124 2124 c, t dbSNP:368254411
2126 2126 a, g dbSNP:138105397
2128 2128 a, c dbSNP:766430404
2145 2145 a, g dbSNP:767717703
2148 2148 a, g, t dbSNP:752896534
2156 2156 a, g dbSNP:778719316
2187 2187 c, t dbSNP:750226582
2193 2193 c, g dbSNP:758352141
2199 2199 c, t dbSNP:780026522
2202 2202 c, t dbSNP:758454149
2203 2203 c, t dbSNP:550322570
2219 2219 a, t dbSNP:768089988
2220 2220 c, t dbSNP:3793801
2229 2229 c, t dbSNP:747791343
2234 2234 c, g dbSNP:201439531
2236 2236 a, g dbSNP:769377082
2237 2237 c, g dbSNP:773591109
2258 2258 -, ctt dbSNP:750942168
2280 2280 a, c dbSNP:145995632
2286 2286 a, g dbSNP:747261452
2292 2292 a, c dbSNP:771548584
2294 2294 a, g dbSNP:535850567
2295 2295 a, c dbSNP:114208185
2298 2298 c, t dbSNP:760123165
2322 2322 c, t dbSNP:767664583
2323 2323 a, c dbSNP:752740939
2324 2324 g, t dbSNP:760920908
2325 2325 a, g dbSNP:566628025
2330 2330 c, g, t dbSNP:79414242
2331 2331 a, g dbSNP:77144546
2336 2336 c, t dbSNP:74334349
2338 2338 a, g dbSNP:751461280
2340 2340 a, g dbSNP:754935493
2358 2358 -, a dbSNP:768461054
2362 2362 a, g dbSNP:780662703
2365 2365 a, g dbSNP:142285373
2372 2372 c, t dbSNP:755783618
2375 2375 a, g dbSNP:114719193
2379 2379 c, g dbSNP:748981265
2384 2384 a, g dbSNP:771354440
2395 2395 c, g dbSNP:774886016
2396 2396 a, c dbSNP:746327923
2402 2402 c, t dbSNP:772755011
2405 2405 c, t dbSNP:374303993
2409 2409 a, g dbSNP:775635830
2414 2414 g, t dbSNP:760725539
2418 2418 a, c dbSNP:764248770
2419 2419 a, c dbSNP:776653200
2420 2420 c, t dbSNP:762055920
2421 2421 a, c dbSNP:766117753
2422 2422 c, t dbSNP:751408195
2423 2423 c, g dbSNP:578155039
2425 2425 a, c dbSNP:754875240
2428 2428 -, c dbSNP:145178233
2428 2428 a, c dbSNP:767416437
2430 2430 c, g dbSNP:759489601
2431 2431 c, t dbSNP:752234656
2434 2434 a, c dbSNP:199684404
2444 2444 -, agcca dbSNP:780315825
2452 2452 c, t dbSNP:755519156

Target ORF information:

RefSeq Version NM_001142929
Organism Homo sapiens (human)
Definition Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001142929

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16386
Accession Version NM_020985.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1893bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product choline O-acetyltransferase isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D82340.1, AC073366.4, BC130615.1 and AF305907.1. On May 21, 2008 this sequence version replaced gi:119433668. Summary: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]. Transcript Variant: This variant (N1) contains alternate 5' exon N and uses an alternate splice site within alternate exon M, and encodes isoform 1 (PMID 9073174). Transcript variants R, N1, N2, M and S encode isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF305909.1, BC130615.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 5' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCAGCAA AAACTCCCAG CAGTGAGGAG TCTGGGCTGC CCAAACTGCC CGTGCCCCCG 
CTGCAGCAGA CCCTGGCCAC GTACCTGCAG TGCATGCGAC ACTTGGTGTC TGAGGAGCAG
TTCAGGAAGA GCCAGGCCAT TGTGCAGCAG TTTGGGGCCC CTGGTGGCCT CGGCGAGACC
CTGCAGCAGA AACTCCTGGA GCGGCAGGAG AAGACAGCCA ACTGGGTGTC TGAGTACTGG
CTGAATGACA TGTATCTCAA CAACCGCCTG GCCCTGCCTG TCAACTCCAG CCCTGCCGTG
ATCTTTGCTC GGCAGCACTT CCCTGGCACC GATGACCAGC TGAGGTTTGC AGCCAGCCTC
ATCTCTGGTG TACTCAGCTA CAAGGCCCTG CTGGACAGCC ACTCCATTCC CACTGACTGT
GCCAAAGGCC AGCTGTCAGG GCAGCCCCTT TGCATGAAGC AATACTATGG GCTCTTCTCC
TCCTACCGGC TCCCCGGCCA TACCCAGGAC ACGCTGGTGG CTCAGAACAG CAGCATCATG
CCGGAGCCTG AGCACGTCAT CGTAGCCTGC TGCAATCAGT TCTTTGTCTT GGATGTTGTC
ATTAATTTCC GCCGTCTCAG TGAGGGGGAT CTGTTCACTC AGTTGAGAAA GATAGTCAAA
ATGGCTTCCA ACGAGGACGA GCGTTTGCCT CCAATTGGCC TGCTGACGTC TGACGGGAGG
AGCGAGTGGG CCGAGGCCAG GACGGTCCTC GTGAAAGACT CCACCAACCG GGACTCGCTG
GACATGATTG AGCGCTGCAT CTGCCTTGTA TGCCTGGACG CGCCAGGAGG CGTGGAGCTC
AGCGACACCC ACAGGGCACT CCAGCTCCTT CACGGCGGAG GCTACAGCAA GAACGGGGCC
AATCGCTGGT ACGACAAGTC CCTGCAGTTT GTGGTGGGCC GAGACGGCAC CTGCGGTGTG
GTGTGCGAAC ACTCCCCATT CGATGGCATC GTCCTGGTGC AGTGCACTGA GCATCTGCTC
AAGCACATGA CGCAGAGCAG CAGGAAGCTG ATCCGAGCAG ACTCCGTCAG CGAGCTCCCC
GCCCCCCGGA GGCTGCGGTG GAAATGCTCC CCGGAAATTC AAGGCCACTT AGCCTCCTCG
GCAGAAAAAC TTCAACGAAT AGTAAAGAAC CTTGACTTCA TTGTCTATAA GTTTGACAAC
TATGGGAAAA CATTCATTAA GAAGCAGAAA TGCAGCCCTG ATGCCTTCAT CCAGGTGGCC
CTCCAGCTGG CCTTCTACAG GCTCCATCGA AGACTGGTGC CCACCTACGA GAGCGCGTCC
ATCCGCCGAT TCCAGGAGGG ACGCGTGGAC AACATCAGAT CGGCCACTCC AGAGGCACTG
GCTTTTGTGA GAGCCGTGAC TGACCACAAG GCTGCTGTGC CAGCTTCTGA GAAGCTTCTG
CTCCTGAAGG ATGCCATCCG TGCCCAGACT GCATACACAG TCATGGCCAT AACAGGGATG
GCCATTGACA ACCACCTGCT GGCACTGCGG GAGCTGGCCC GGGCCATGTG CAAGGAGCTG
CCCGAGATGT TCATGGATGA AACCTACCTG ATGAGCAACC GGTTTGTCCT CTCCACTAGC
CAGGTGCCCA CAACCACGGA GATGTTCTGC TGCTATGGTC CTGTGGTCCC AAATGGGTAT
GGTGCCTGCT ACAACCCCCA GCCAGAGACC ATCCTTTTCT GCATCTCTAG CTTTCACAGC
TGCAAAGAGA CTTCTTCTAG CAAGTTTGCA AAAGCTGTGG AAGAAAGCCT CATTGACATG
AGAGACCTCT GCAGTCTGCT GCCGCCTACT GAGAGCAAGC CATTGGCAAC AAAGGAAAAA
GCCACGAGGC CCAGCCAGGG ACACCAACCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_066265.3
CDS336..2228
Misc Feature(1)75..77(+)
Misc Feature(2)372..2141(+)
Exon (1)1..95
Gene:CHAT
Gene Synonym:
Exon (2)96..267
Gene:CHAT
Gene Synonym:
Exon (3)268..368
Gene:CHAT
Gene Synonym:
Exon (4)369..560
Gene:CHAT
Gene Synonym:
Exon (5)561..679
Gene:CHAT
Gene Synonym:
Exon (6)680..733
Gene:CHAT
Gene Synonym:
Exon (7)734..914
Gene:CHAT
Gene Synonym:
Exon (8)915..1092
Gene:CHAT
Gene Synonym:
Exon (9)1093..1262
Gene:CHAT
Gene Synonym:
Exon (10)1263..1363
Gene:CHAT
Gene Synonym:
Exon (11)1364..1492
Gene:CHAT
Gene Synonym:
Exon (12)1493..1615
Gene:CHAT
Gene Synonym:
Exon (13)1616..1757
Gene:CHAT
Gene Synonym:
Exon (14)1758..1820
Gene:CHAT
Gene Synonym:
Exon (15)1821..1958
Gene:CHAT
Gene Synonym:
Exon (16)1959..2286
Gene:CHAT
Gene Synonym:
Translation
Position Chain Variation Link
9 9 g, t dbSNP:77723675
29 29 a, g dbSNP:751757192
38 38 g, t dbSNP:3729496
45 45 c, t dbSNP:553208755
65 65 a, g dbSNP:571385948
66 66 c, t dbSNP:538800349
90 90 a, g dbSNP:3810947
97 97 a, c dbSNP:370609858
98 98 c, g dbSNP:768179285
100 100 g, t dbSNP:776411377
122 122 c, g dbSNP:3810948
129 129 g, t dbSNP:765746009
134 134 a, t dbSNP:773735383
137