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CHKB cDNA ORF clone, Homo sapiens (human)

Gene Symbol CHKB
Entrez Gene ID 1120
Full Name choline kinase beta
Synonyms CHETK, CHKL, CK, CKB, CKEKB, EK, EKB, MDCMC
General protein information
Preferred Names
choline/ethanolamine kinase
Names
choline/ethanolamine kinase
ethanolamine kinase beta
choline kinase-like protein
NP_005189.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q13.33

Summary Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]. lac of sum
Disorder MIM:

612395


mRNA and Protein(s)

mRNA Protein Name
NM_005198 NP_005189 choline/ethanolamine kinase



Homo sapiens (human) CHKB NP_005189.2
Pan troglodytes (chimpanzee) CHKB XP_001144556.1
Macaca mulatta (Rhesus monkey) LOC716377 XP_002808018.1
Canis lupus familiaris (dog) CHKB XP_848257.1
Bos taurus (cattle) CHKB NP_001193023.1
Mus musculus (house mouse) Chkb NP_031718.1
Rattus norvegicus (Norway rat) Chkb NP_058873.1
Danio rerio (zebrafish) chkb NP_001093482.1
Drosophila melanogaster (fruit fly) CG2201 NP_724349.1
Caenorhabditis elegans cka-1 NP_001129849.1
Arabidopsis thaliana (thale cress) AT1G74320 NP_177572.2
Xenopus (Silurana) tropicalis (western clawed frog) chkb NP_001120148.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHKB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHKB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19326
NM_005198 Homo sapiens choline kinase beta (CHKB), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $189.50
$379.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19326
Clone ID Related Accession (Same CDS sequence) NM_005198
Accession Version NM_005198.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product choline/ethanolamine kinase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB106393.1, AB029886.1 and BE676287.1. This sequence is a reference standard in the RefSeqGene project. On Jun 27, 2009 this sequence version replaced gi:23238259. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL096780.1, BC082263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCGGCCG AGGCGACAGC TGTGGCCGGA AGCGGGGCTG TTGGCGGCTG CCTGGCCAAA 
GACGGCTTGC AGCAGTCTAA GTGCCCGGAC ACTACCCCAA AACGGCGGCG CGCCTCGTCG
CTGTCGCGTG ACGCCGAGCG CCGAGCCTAC CAATGGTGCC GGGAGTACTT GGGCGGGGCC
TGGCGCCGAG TGCAGCCCGA GGAGCTGAGG GTTTACCCCG TGAGCGGAGG CCTCAGCAAC
CTGCTCTTCC GCTGCTCGCT CCCGGACCAC CTGCCCAGCG TTGGCGAGGA GCCCCGGGAG
GTGCTTCTGC GGCTGTACGG AGCCATCTTG CAGGGCGTGG ACTCCCTGGT GCTAGAAAGC
GTGATGTTCG CCATACTTGC GGAGCGGTCG CTGGGGCCCC AGCTGTACGG AGTCTTCCCA
GAGGGCCGGC TGGAACAGTA CATCCCAAGT CGGCCATTGA AAACTCAAGA GCTTCGAGAG
CCAGTGTTGT CAGCAGCCAT TGCCACGAAG ATGGCGCAAT TTCATGGCAT GGAGATGCCT
TTCACCAAGG AGCCCCACTG GCTGTTTGGG ACCATGGAGC GGTACCTAAA ACAGATCCAG
GACCTGCCCC CAACTGGCCT CCCTGAGATG AACCTGCTGG AGATGTACAG CCTGAAGGAT
GAGATGGGCA ACCTCAGGAA GTTACTAGAG TCTACCCCAT CGCCAGTCGT CTTCTGCCAC
AATGACATCC AGGAAGGGAA CATCTTGCTG CTCTCAGAGC CAGAAAATGC TGACAGCCTC
ATGCTGGTGG ACTTCGAGTA CAGCAGTTAT AACTATAGGG GCTTTGACAT TGGGAACCAT
TTTTGTGAGT GGGTTTATGA TTATACTCAC GAGGAATGGC CTTTCTACAA AGCAAGGCCC
ACAGACTACC CCACTCAAGA ACAGCAGTTG CATTTTATTC GTCATTACCT GGCAGAGGCA
AAGAAAGGTG AGACCCTCTC CCAAGAGGAG CAGAGAAAAC TGGAAGAAGA TTTGCTGGTA
GAAGTCAGTC GGTATGCTCT GGCATCCCAT TTCTTCTGGG GTCTGTGGTC CATCCTCCAG
GCATCCATGT CCACCATAGA ATTTGGTTAC TTGGACTATG CCCAGTCTCG GTTCCAGTTC
TACTTCCAGC AGAAGGGGCA GCTGACCAGT GTCCACTCCT CATCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005189.2
CDS219..1406
Misc Feature(1)105..107(+)
Misc Feature(2)222..224(+)
Misc Feature(3)387..1382(+)
Misc Feature(4)423..1373(+)
Misc Feature(5)441..1286(+)
Misc Feature(6)441..1010(+)
Misc Feature(7)447..1286(+)
Misc Feature(8)447..455(+)
Misc Feature(9)567..770(+)
Exon (1)1..442
Gene:CHKB
Gene Synonym:
Exon (2)443..551
Gene:CHKB
Gene Synonym:
Exon (3)552..665
Gene:CHKB
Gene Synonym:
Exon (4)666..799
Gene:CHKB
Gene Synonym:
Exon (5)800..895
Gene:CHKB
Gene Synonym:
Exon (6)896..954
Gene:CHKB
Gene Synonym:
Exon (7)955..1036
Gene:CHKB
Gene Synonym:
Exon (8)1037..1145
Gene:CHKB
Gene Synonym:
Exon (9)1146..1249
Gene:CHKB
Gene Synonym:
Exon (10)1250..1331
Gene:CHKB
Gene Synonym:
Exon (11)1332..1629
Gene:CHKB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005198
Organism Homo sapiens (human)
Definition Homo sapiens choline kinase beta (CHKB), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005198

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGCGGCCG AGGCGACAGC TGTGGCCGGA AGCGGGGCTG TTGGCGGCTG CCTGGCCAAA 
GACGGCTTGC AGCAGTCTAA GTGCCCGGAC ACTACCCCAA AACGGCGGCG CGCCTCGTCG
CTGTCGCGTG ACGCCGAGCG CCGAGCCTAC CAATGGTGCC GGGAGTACTT GGGCGGGGCC
TGGCGCCGAG TGCAGCCCGA GGAGCTGAGG GTTTACCCCG TGAGCGGAGG CCTCAGCAAC
CTGCTCTTCC GCTGCTCGCT CCCGGACCAC CTGCCCAGCG TTGGCGAGGA GCCCCGGGAG
GTGCTTCTGC GGCTGTACGG AGCCATCTTG CAGGGCGTGG ACTCCCTGGT GCTAGAAAGC
GTGATGTTCG CCATACTTGC GGAGCGGTCG CTGGGGCCCC AGCTGTACGG AGTCTTCCCA
GAGGGCCGGC TGGAACAGTA CATCCCAAGT CGGCCATTGA AAACTCAAGA GCTTCGAGAG
CCAGTGTTGT CAGCAGCCAT TGCCACGAAG ATGGCGCAAT TTCATGGCAT GGAGATGCCT
TTCACCAAGG AGCCCCACTG GCTGTTTGGG ACCATGGAGC GGTACCTAAA ACAGATCCAG
GACCTGCCCC CAACTGGCCT CCCTGAGATG AACCTGCTGG AGATGTACAG CCTGAAGGAT
GAGATGGGCA ACCTCAGGAA GTTACTAGAG TCTACCCCAT CGCCAGTCGT CTTCTGCCAC
AATGACATCC AGGAAGGGAA CATCTTGCTG CTCTCAGAGC CAGAAAATGC TGACAGCCTC
ATGCTGGTGG ACTTCGAGTA CAGCAGTTAT AACTATAGGG GCTTTGACAT TGGGAACCAT
TTTTGTGAGT GGGTTTATGA TTATACTCAC GAGGAATGGC CTTTCTACAA AGCAAGGCCC
ACAGACTACC CCACTCAAGA ACAGCAGTTG CATTTTATTC GTCATTACCT GGCAGAGGCA
AAGAAAGGTG AGACCCTCTC CCAAGAGGAG CAGAGAAAAC TGGAAGAAGA TTTGCTGGTA
GAAGTCAGTC GGTATGCTCT GGCATCCCAT TTCTTCTGGG GTCTGTGGTC CATCCTCCAG
GCATCCATGT CCACCATAGA ATTTGGTTAC TTGGACTATG CCCAGTCTCG GTTCCAGTTC
TACTTCCAGC AGAAGGGGCA GCTGACCAGT GTCCACTCCT CATCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Megaconial congenital muscular dystrophy due to loss-of-function mutations in choline kinase beta
Curr. Opin. Neurol. 26 (5), 536-543 (2013)
Mitsuhashi S and Nishino I.


book

Muscular dystrophy with large mitochondria associated with mutations in the CHKB gene in three British patients: extending the clinical and pathological phenotype
Neuromuscul. Disord. 23 (7), 549-556 (2013)
Quinlivan R, Mitsuahashi S, Sewry C, Cirak S, Aoyama C, Mooore D, Abbs S, Robb S, Newton T, Moss C, Birchall D, Sugimoto H, Bushby K, Guglieri M, Muntoni F, Nishino I and Straub V.


book

TCRA, P2RY11, and CPT1B/CHKB associations in Chinese narcolepsy
Sleep Med. 13 (3), 269-272 (2012)
Han F, Lin L, Li J, Aran A, Dong SX, An P, Zhao L, Li QY, Yan H, Wang JS, Gao HY, Li M, Gao ZC, Strohl KP and Mignot E.


book

A congenital muscular dystrophy with mitochondrial structural abnormalities caused by defective de novo phosphatidylcholine biosynthesis
Am. J. Hum. Genet. 88 (6), 845-851 (2011)
Mitsuhashi S, Ohkuma A, Talim B, Karahashi M, Koumura T, Aoyama C, Kurihara M, Quinlivan R, Sewry C, Mitsuhashi H, Goto K, Koksal B, Kale G, Ikeda K, Taguchi R, Noguchi S, Hayashi YK, Nonaka I, Sher RB, Sugimoto H, Nakagawa Y, Cox GA, Topaloglu H and Nishino I.


book

Distinct choline metabolic profiles are associated with differences in gene expression for basal-like and luminal-like breast cancer xenograft models
BMC Cancer 10, 433 (2010)
Moestue SA, Borgan E, Huuse EM, Lindholm EM, Sitter B, Borresen-Dale AL, Engebraaten O, Maelandsmo GM and Gribbestad IS.


book

Structure and function of choline kinase isoforms in mammalian cells
Prog. Lipid Res. 43 (3), 266-281 (2004)
Aoyama C, Liao H and Ishidate K.


book

Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping
DNA Res. 8 (1), 1-9 (2001)
Hirosawa M, Nagase T, Murahashi Y, Kikuno R and Ohara O.


book

Novel expression of equivocal messages containing both regions of choline/ethanolamine kinase and muscle type carnitine palmitoyltransferase I
J. Biol. Chem. 275 (41), 31739-31746 (2000)
Yamazaki N, Shinohara Y, Kajimoto K, Shindo M and Terada H.


book

Choline/ethanolamine kinase from mammalian tissues
Biochim. Biophys. Acta 1348 (1-2), 70-78 (1997)
Ishidate K.


book

Congenital Muscular Dystrophy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Sparks,S., Quijano-Roy,S., Harper,A., Rutkowski,A., Gordon,E., Hoffman,E.P. and Pegoraro,E.


 
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