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PRAF2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PRAF2
Entrez Gene ID 11230
Full Name PRA1 domain family, member 2
Synonyms JM4
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp11.23

Summary lac of sum
Disorder MIM:

300840

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_007213 NP_009144 PRA1 family protein 2



Homo sapiens (human) PRAF2 NP_009144.1
Pan troglodytes (chimpanzee) PRAF2 XP_528974.2
Canis lupus familiaris (dog) PRAF2 XP_538032.1
Bos taurus (cattle) PRAF2 NP_001039474.1
Mus musculus (house mouse) Praf2 NP_613068.1
Rattus norvegicus (Norway rat) Praf2 NP_001102483.1
Danio rerio (zebrafish) praf2 NP_001017698.1

Component

ID Name Evidence
GO:0005768 endosome IEA
GO:0010008 endosome membrane IEA
GO:0016020 membrane IEA
GO:0016021 integral to membrane IEA

Process

ID Name Evidence
GO:0015031 protein transport IEA
GO:0015813 L-glutamate transport ISS

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRAF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRAF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu12218
NM_007213 Homo sapiens PRA1 domain family, member 2 (PRAF2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu12218
Accession Version NM_007213.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product PRA1 family protein 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB155577.1, AJ005896.1, AC231657.2 and AA720948.1. On Aug 21, 2013 this sequence version replaced gi:6005793. ##Evidence-Data-START## Transcript exon combination :: AJ005896.1, BC021213.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962341 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGTCGGAGG TGCGGCTGCC ACCGCTACGC GCCCTGGACG ACTTTGTTCT GGGGTCGGCG 
CGTCTGGCGG CTCCGGATCC ATGCGACCCG CAGCGATGGT GCCACCGCGT CATCAACAAC
CTCCTCTACT ACCAAACCAA CTACCTTCTC TGCTTCGGCA TCGGCCTCGC TCTCGCCGGG
TACGTGCGGC CACTTCATAC GCTCCTGAGC GCGCTGGTAG TGGCGGTGGC CCTCGGCGTG
CTGGTGTGGG CAGCTGAGAC CCGCGCAGCT GTGCGCCGCT GCCGCCGCAG CCACCCTGCA
GCCTGCCTGG CCGCAGTGCT TGCCGTCGGC CTCCTGGTGC TCTGGGTCGC GGGCGGCGCT
TGCACCTTCC TGTTCAGCAT CGCCGGGCCG GTGCTTCTGA TCCTGGTGCA CGCCTCGTTG
CGCCTGCGCA ACCTTAAGAA CAAGATTGAG AACAAGATCG AGAGCATTGG TCTCAAGCGG
ACGCCAATGG GCCTGCTACT AGAGGCACTG GGACAAGAGC AGGAGGCTGG ATCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_009144.1
CDS59..595
Misc Feature(1)14..16(+)
Misc Feature(2)59..529(+)
Misc Feature(3)182..244(+)
Misc Feature(4)251..313(+)
Misc Feature(5)347..415(+)
Misc Feature(6)425..478(+)
Exon (1)1..237
Gene:PRAF2
Gene Synonym:
Exon (2)238..455
Gene:PRAF2
Gene Synonym:
Exon (3)456..1308
Gene:PRAF2
Gene Synonym:
Translation
Position Chain Variation Link
329 329 g, t dbSNP:111976501
731 731 c, t dbSNP:41309731
954 954 a, g dbSNP:1802957

Target ORF information:

RefSeq Version NM_007213
Organism Homo sapiens (human)
Definition Homo sapiens PRA1 domain family, member 2 (PRAF2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007213

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGGAGG TGCGGCTGCC ACCGCTACGC GCCCTGGACG ACTTTGTTCT GGGGTCGGCG 
CGTCTGGCGG CTCCGGATCC ATGCGACCCG CAGCGATGGT GCCACCGCGT CATCAACAAC
CTCCTCTACT ACCAAACCAA CTACCTTCTC TGCTTCGGCA TCGGCCTCGC TCTCGCCGGG
TACGTGCGGC CACTTCATAC GCTCCTGAGC GCGCTGGTAG TGGCGGTGGC CCTCGGCGTG
CTGGTGTGGG CAGCTGAGAC CCGCGCAGCT GTGCGCCGCT GCCGCCGCAG CCACCCTGCA
GCCTGCCTGG CCGCAGTGCT TGCCGTCGGC CTCCTGGTGC TCTGGGTCGC GGGCGGCGCT
TGCACCTTCC TGTTCAGCAT CGCCGGGCCG GTGCTTCTGA TCCTGGTGCA CGCCTCGTTG
CGCCTGCGCA ACCTTAAGAA CAAGATTGAG AACAAGATCG AGAGCATTGG TCTCAAGCGG
ACGCCAATGG GCCTGCTACT AGAGGCACTG GGACAAGAGC AGGAGGCTGG ATCCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

PRAF2 stimulates cell proliferation and migration and predicts poor prognosis in neuroblastoma
Int. J. Oncol. 42 (4), 1408-1416 (2013)
Yco LP, Geerts D, Koster J and Bachmann AS.


book

Subcellular distribution and expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in malignant glioma: Influence on cell survival and migration
Cancer Sci. 101 (7), 1624-1631 (2010)
Borsics T, Lundberg E, Geerts D, Koomoa DL, Koster J, Wester K and Bachmann AS.


book

Praf2 is a novel Bcl-xL/Bcl-2 interacting protein with the ability to modulate survival of cancer cells
PLoS ONE 5 (12), E15636 (2010)
Vento MT, Zazzu V, Loffreda A, Cross JR, Downward J, Stoppelli MP and Iaccarino I.


book

Expression profile of PRAF2 in the human brain and enrichment in synaptic vesicles
Neurosci. Lett. 436 (2), 171-176 (2008)
Koomoa DL, Go RC, Wester K and Bachmann AS.


book

Expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in neuroblastoma: correlation with clinical features, cellular localization, and cerulenin-mediated apoptosis regulation
Clin. Cancer Res. 13 (21), 6312-6319 (2007)
Geerts D, Wallick CJ, Koomoa DL, Koster J, Versteeg R, Go RC and Bachmann AS.


book

Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags
Biochem. Biophys. Res. Commun. 345 (3), 1022-1032 (2006)
Wang AG, Yoon SY, Oh JH, Jeon YJ, Kim M, Kim JM, Byun SS, Yang JO, Kim JH, Kim DG, Yeom YI, Yoo HS, Kim YS and Kim NS.


book

Genomic organization, expression profile, and characterization of the new protein PRA1 domain family, member 2 (PRAF2)
Gene 371 (1), 154-165 (2006)
Fo CS, Coleman CS, Wallick CJ, Vine AL and Bachmann AS.


book

JM4 is a four-transmembrane protein binding to the CCR5 receptor
FEBS Lett. 579 (7), 1751-1758 (2005)
Schweneker M, Bachmann AS and Moelling K.


 
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