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SLC46A1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SLC46A1
Entrez Gene ID 113235
Full Name solute carrier family 46 (folate transporter), member 1
Synonyms G21, HCP1, PCFT
General protein information
Preferred Names
proton-coupled folate transporter
Names
proton-coupled folate transporter
G21
PCFT/HCP1
heme carrier protein 1
solute carrier family 46 member 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q11.2

Summary This gene encodes a transmembrane proton-coupled folate transporter protein that facilitates the movement of folate and antifolate substrates across cell membranes, optimally in acidic pH environments. This protein is also expressed in the brain and choroid plexus where it transports folates into the central nervous system. This protein further functions as a heme transporter in duodenal enterocytes, and potentially in other tissues like liver and kidney. Its localization to the apical membrane or cytoplasm of intestinal cells is modulated by dietary iron levels. Mutations in this gene are associated with autosomal recessive hereditary folate malabsorption disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]. lac of sum
Disorder MIM:

611672

Disorder Html: Folate malabsorption, hereditary, 229050 (3)





The following SLC46A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC46A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu50063 XM_005277786 PREDICTED: Homo sapiens solute carrier family 46 (folate transporter), member 1 (SLC46A1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OHu16708 NM_080669 Homo sapiens solute carrier family 46 (folate transporter), member 1 (SLC46A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00
OHu16737 NM_001242366 Homo sapiens solute carrier family 46 (folate transporter), member 1 (SLC46A1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.30
$319.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu50063
Accession Version XM_005277786.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1248bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product proton-coupled folate transporter isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530432866. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA
GGATGCTGGA AAAGGCTGAT CCTCACCTCG AGTTCCAGCA GTTTCCCCAG AGCCCCTGAT
CTGCCTGGAC CAGAAGACAG AGGGCAAGAG GAGCAAAGTG AACACCAAGC AACTGGAGGT
CTGCAGCTGG AAGCCCAGCC CACAGCAGGA CAAGCAACTC TTGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_005277843.1
CDS99..1346
Misc Feature(1)315..>788(+)
Misc Feature(2)378..>971(+)
Translation
Position Chain Variation Link
121 121 -, c dbSNP:397515574
292 292 -, g dbSNP:397515573
292 292 -, g dbSNP:80338769
295 295 aa, gc dbSNP:154623632
302 302 -, cc dbSNP:397515391
435 435 a, c, t dbSNP:80338770
537 537 c, g dbSNP:80338771
564 564 g, t dbSNP:281875210
996 996 a, g dbSNP:201540617
1052 1052 c, g dbSNP:80338772
1102 1102 a, c dbSNP:281875208
1110 1110 a, c, g dbSNP:281875209
1471 1471 c, g dbSNP:4990808
1647 1647 c, t dbSNP:773355650
1755 1755 c, g dbSNP:770166353
1908 1908 c, t dbSNP:41297123
1929 1929 a, t dbSNP:112801276
2165 2165 a, g dbSNP:2239907
2388 2388 a, t dbSNP:62065282
2480 2480 a, g dbSNP:41297909
2644 2644 c, t dbSNP:2239908

Target ORF information:

RefSeq Version XM_005277786
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 46 (folate transporter), member 1 (SLC46A1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005277786

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA
GGATGCTGGA AAAGGCTGAT CCTCACCTCG AGTTCCAGCA GTTTCCCCAG AGCCCCTGAT
CTGCCTGGAC CAGAAGACAG AGGGCAAGAG GAGCAAAGTG AACACCAAGC AACTGGAGGT
CTGCAGCTGG AAGCCCAGCC CACAGCAGGA CAAGCAACTC TTGTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16708
Accession Version NM_080669.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1380bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product proton-coupled folate transporter isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA188147.1, DB121588.1, AK054669.1, AK074161.1, DA436636.1, DA527125.1, DA013269.1, BC022100.1 and BC065365.1. On Aug 18, 2013 this sequence version replaced gi:334688814. Summary: This gene encodes a transmembrane proton-coupled folate transporter protein that facilitates the movement of folate and antifolate substrates across cell membranes, optimally in acidic pH environments. This protein is also expressed in the brain and choroid plexus where it transports folates into the central nervous system. This protein further functions as a heme transporter in duodenal enterocytes, and potentially in other tissues like liver and kidney. Its localization to the apical membrane or cytoplasm of intestinal cells is modulated by dietary iron levels. Mutations in this gene are associated with autosomal recessive hereditary folate malabsorption disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]. Transcript Variant: This variant (1, also known as HCP-1A) encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA
GGATATGGGT TGCTTTTCCT GTCATTAGTC ATCACACCTG TCATCCGGGC TAAACTCTCC
AAGCTGGTGA GAGAGACAGA GCAGGGTGCT CTCTTTTCTG CTGTGGCCTG TGTGAATAGC
CTGGCCATGC TGACGGCCTC CGGCATCTTC AACTCACTCT ACCCAGCCAC TCTGAACTTT
ATGAAGGGGT TCCCCTTCCT CCTGGGAGCT GGCCTCCTGC TCATCCCGGC TGTTCTGATT
GGGATGCTGG AAAAGGCTGA TCCTCACCTC GAGTTCCAGC AGTTTCCCCA GAGCCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_542400.2
CDS99..1478
Misc Feature(1)99..101(+)
Misc Feature(2)315..1427(+)
Misc Feature(3)315..1307(+)
Misc Feature(4)357..419(+)
Misc Feature(5)378..1319(+)
Misc Feature(6)441..503(+)
Misc Feature(7)543..605(+)
Misc Feature(8)645..707(+)
Misc Feature(9)729..791(+)
Misc Feature(10)897..959(+)
Misc Feature(11)1005..1073(+)
Misc Feature(12)1107..1169(+)
Misc Feature(13)1173..1235(+)
Misc Feature(14)1266..1328(+)
Misc Feature(15)1365..1427(+)
Misc Feature(16)1470..1472(+)
Exon (1)1..326
Gene:SLC46A1
Gene Synonym:
Exon (2)327..1179
Gene:SLC46A1
Gene Synonym:
Exon (3)1180..1263
Gene:SLC46A1
Gene Synonym:
Exon (4)1264..1420
Gene:SLC46A1
Gene Synonym:
Exon (5)1421..6492
Gene:SLC46A1
Gene Synonym:
Translation
Position Chain Variation Link
121 121 -, c dbSNP:397515574
292 292 -, g dbSNP:397515573
292 292 -, g dbSNP:80338769
295 295 aa, gc dbSNP:154623632
302 302 -, cc dbSNP:397515391
435 435 a, c, t dbSNP:80338770
537 537 c, g dbSNP:80338771
564 564 g, t dbSNP:281875210
996 996 a, g dbSNP:201540617
1052 1052 c, g dbSNP:80338772
1102 1102 a, c dbSNP:281875208
1110 1110 a, c, g dbSNP:281875209
1215 1215 c, g dbSNP:75413886
1224 1224 c, t dbSNP:80338773
1225 1225 a, g dbSNP:281875211
1313 1313 a, g dbSNP:761549800
1324 1324 -, t dbSNP:561780114
1325 1325 c, t dbSNP:199895119
1327 1327 c, t dbSNP:201932339
1372 1372 c, g dbSNP:80338774
1712 1712 c, g dbSNP:4990808
1888 1888 c, t dbSNP:773355650
1996 1996 c, g dbSNP:770166353
2149 2149 c, t dbSNP:41297123
2170 2170 a, t dbSNP:112801276
2406 2406 a, g dbSNP:2239907
2629 2629 a, t dbSNP:62065282
2721 2721 a, g dbSNP:41297909
2885 2885 c, t dbSNP:2239908
3127 3127 a, g dbSNP:779490495
3270 3270 c, g dbSNP:370430108
3272 3272 c, g dbSNP:377025828
3273 3273 a, c dbSNP:200529579
3274 3274 -, agc, gca dbSNP:34137807
3601 3601 c, g dbSNP:7225850
3726 3726 c, t dbSNP:8082600
4036 4036 c, t dbSNP:750056802
4115 4115 c, t dbSNP:764863596
4331 4331 a, g dbSNP:739439
4420 4420 c, t dbSNP:756767
4487 4487 g, t dbSNP:2239910
4540 4540 a, c dbSNP:2239911
4613 4613 a, g dbSNP:762983414
4705 4705 a, g dbSNP:56736971
4726 4726 a, c dbSNP:773480947
4744 4744 a, g dbSNP:73273104
4765 4765 a, g dbSNP:762025374
4856 4856 a, g dbSNP:61456782
4857 4857 a, c dbSNP:61629513
5190 5190 a, g dbSNP:776939252
5425 5425 a, g dbSNP:8081240
5508 5508 c, t dbSNP:8079943
5541 5541 c, t dbSNP:192565566
5794 5794 c, g dbSNP:117397871
6062 6062 c, t dbSNP:116278566
6134 6134 a, c dbSNP:745424962
6258 6258 c, t dbSNP:1128161
6316 6316 a, g dbSNP:777666113
6350 6350 a, t dbSNP:756022651
6359 6359 a, c dbSNP:752650508
6362 6362 g, t dbSNP:1128162
6423 6423 c, t dbSNP:763076349

Target ORF information:

RefSeq Version NM_080669
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 46 (folate transporter), member 1 (SLC46A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_080669

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA
GGATATGGGT TGCTTTTCCT GTCATTAGTC ATCACACCTG TCATCCGGGC TAAACTCTCC
AAGCTGGTGA GAGAGACAGA GCAGGGTGCT CTCTTTTCTG CTGTGGCCTG TGTGAATAGC
CTGGCCATGC TGACGGCCTC CGGCATCTTC AACTCACTCT ACCCAGCCAC TCTGAACTTT
ATGAAGGGGT TCCCCTTCCT CCTGGGAGCT GGCCTCCTGC TCATCCCGGC TGTTCTGATT
GGGATGCTGG AAAAGGCTGA TCCTCACCTC GAGTTCCAGC AGTTTCCCCA GAGCCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16737
Accession Version NM_001242366.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1296bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product proton-coupled folate transporter isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA188147.1, BC010691.1, AK074161.1, DA436636.1, DA527125.1, DA013269.1, BC022100.1 and BC065365.1. On Aug 18, 2013 this sequence version replaced gi:334688815. Summary: This gene encodes a transmembrane proton-coupled folate transporter protein that facilitates the movement of folate and antifolate substrates across cell membranes, optimally in acidic pH environments. This protein is also expressed in the brain and choroid plexus where it transports folates into the central nervous system. This protein further functions as a heme transporter in duodenal enterocytes, and potentially in other tissues like liver and kidney. Its localization to the apical membrane or cytoplasm of intestinal cells is modulated by dietary iron levels. Mutations in this gene are associated with autosomal recessive hereditary folate malabsorption disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]. Transcript Variant: This variant (2, also known as HCP-1B) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment (containing one of the transmembrane domains) compared to isoform 1. This isoform was shown to have a different subcellular localization compared to the longer isoform 1 (PMID:17947394). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA
GGTGCTCTCT TTTCTGCTGT GGCCTGTGTG AATAGCCTGG CCATGCTGAC GGCCTCCGGC
ATCTTCAACT CACTCTACCC AGCCACTCTG AACTTTATGA AGGGGTTCCC CTTCCTCCTG
GGAGCTGGCC TCCTGCTCAT CCCGGCTGTT CTGATTGGGA TGCTGGAAAA GGCTGATCCT
CACCTCGAGT TCCAGCAGTT TCCCCAGAGC CCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001229295.1
CDS99..1394
Misc Feature(1)315..>788(+)
Misc Feature(2)378..>971(+)
Exon (1)1..326
Gene:SLC46A1
Gene Synonym:
Exon (2)327..1179
Gene:SLC46A1
Gene Synonym:
Exon (3)1180..1336
Gene:SLC46A1
Gene Synonym:
Exon (4)1337..6408
Gene:SLC46A1
Gene Synonym:
Translation
Position Chain Variation Link
121 121 -, c dbSNP:397515574
292 292 -, g dbSNP:397515573
292 292 -, g dbSNP:80338769
295 295 aa, gc dbSNP:154623632
302 302 -, cc dbSNP:397515391
435 435 a, c, t dbSNP:80338770
537 537 c, g dbSNP:80338771
564 564 g, t dbSNP:281875210
996 996 a, g dbSNP:201540617
1052 1052 c, g dbSNP:80338772
1102 1102 a, c dbSNP:281875208
1110 1110 a, c, g dbSNP:281875209
1229 1229 a, g dbSNP:761549800
1240 1240 -, t dbSNP:561780114
1241 1241 c, t dbSNP:199895119
1243 1243 c, t dbSNP:201932339
1288 1288 c, g dbSNP:80338774
1628 1628 c, g dbSNP:4990808
1804 1804 c, t dbSNP:773355650
1912 1912 c, g dbSNP:770166353
2065 2065 c, t dbSNP:41297123
2086 2086 a, t dbSNP:112801276
2322 2322 a, g dbSNP:2239907
2545 2545 a, t dbSNP:62065282
2637 2637 a, g dbSNP:41297909
2801 2801 c, t dbSNP:2239908
3043 3043 a, g dbSNP:779490495
3186 3186 c, g dbSNP:370430108
3188 3188 c, g dbSNP:377025828
3189 3189 a, c dbSNP:200529579
3190 3190 -, agc, gca dbSNP:34137807
3517 3517 c, g dbSNP:7225850
3642 3642 c, t dbSNP:8082600
3952 3952 c, t dbSNP:750056802
4031 4031 c, t dbSNP:764863596
4247 4247 a, g dbSNP:739439
4336 4336 c, t dbSNP:756767
4403 4403 g, t dbSNP:2239910
4456 4456 a, c dbSNP:2239911
4529 4529 a, g dbSNP:762983414
4621 4621 a, g dbSNP:56736971
4642 4642 a, c dbSNP:773480947
4660 4660 a, g dbSNP:73273104
4681 4681 a, g dbSNP:762025374
4772 4772 a, g dbSNP:61456782
4773 4773 a, c dbSNP:61629513
5106 5106 a, g dbSNP:776939252
5341 5341 a, g dbSNP:8081240
5424 5424 c, t dbSNP:8079943
5457 5457 c, t dbSNP:192565566
5710 5710 c, g dbSNP:117397871
5978 5978 c, t dbSNP:116278566
6050 6050 a, c dbSNP:745424962
6174 6174 c, t dbSNP:1128161
6232 6232 a, g dbSNP:777666113
6266 6266 a, t dbSNP:756022651
6275 6275 a, c dbSNP:752650508
6278 6278 g, t dbSNP:1128162
6339 6339 c, t dbSNP:763076349

Target ORF information:

RefSeq Version NM_001242366
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 46 (folate transporter), member 1 (SLC46A1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001242366

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGGGGA GCGCGAGCCC CCCGGAAAAG CCCCGCGCCC GCCCTGCGGC TGCCGTGCTG 
TGCCGGGGCC CGGTAGAGCC GCTGGTCTTC CTGGCCAACT TTGCCTTGGT CCTGCAGGGC
CCGCTCACCA CGCAGTATCT GTGGCACCGC TTCAGCGCCG ACCTCGGCTA CAATGGCACC
CGCCAAAGGG GGGGCTGCAG CAACCGCAGC GCGGACCCCA CCATGCAGGA AGTGGAGACC
CTTACCTCCC ACTGGACCCT CTACATGAAC GTGGGCGGCT TCCTGGTGGG GCTCTTCTCG
TCCACCCTGC TGGGAGCTTG GAGCGACAGT GTGGGCCGCC GCCCGCTGCT AGTGCTGGCC
TCGCTGGGCC TGCTGCTCCA GGCCCTAGTG TCCGTTTTTG TGGTGCAGCT GCAGCTCCAC
GTCGGCTACT TCGTGCTGGG TCGCATCCTT TGTGCCCTCC TCGGCGACTT CGGTGGCCTT
CTGGCTGCTA GCTTTGCGTC CGTGGCAGAT GTCAGCTCCA GTCGCAGCCG CACCTTCCGG
ATGGCCCTGC TGGAAGCCAG CATCGGGGTG GCTGGGATGC TGGCAAGCCT CCTCGGGGGC
CACTGGCTCC GGGCCCAGGG TTATGCCAAC CCCTTCTGGC TGGCCTTGGC CTTGCTGATA
GCCATGACTC TCTATGCAGC TTTCTGCTTT GGTGAGACCT TAAAGGAGCC AAAGTCCACC
CGGCTCTTCA CGTTCCGTCA CCACCGATCC ATTGTCCAGC TCTATGTGGC TCCCGCCCCA
GAGAAGTCCA GGAAACATTT AGCCCTCTAC TCACTGGCCA TCTTCGTGGT GATCACTGTG
CACTTTGGGG CCCAGGACAT CTTAACCCTT TATGAACTAA GCACACCCCT CTGCTGGGAC
TCCAAACTAA TCGGCTATGG TTCTGCAGCT CAGCATCTCC CCTACCTCAC CAGCCTGCTG
GCCCTGAAGC TCCTGCAGTA CTGCCTGGCC GATGCCTGGG TAGCTGAGAT CGGCCTGGCC
TTCAACATCC TGGGGATGGT GGTCTTTGCC TTTGCCACTA TCACGCCTCT CATGTTCACA
GGTGCTCTCT TTTCTGCTGT GGCCTGTGTG AATAGCCTGG CCATGCTGAC GGCCTCCGGC
ATCTTCAACT CACTCTACCC AGCCACTCTG AACTTTATGA AGGGGTTCCC CTTCCTCCTG
GGAGCTGGCC TCCTGCTCAT CCCGGCTGTT CTGATTGGGA TGCTGGAAAA GGCTGATCCT
CACCTCGAGT TCCAGCAGTT TCCCCAGAGC CCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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