Email to GenScript

CHRNA1 cholinergic receptor, nicotinic, alpha 1 (muscle) [Homo sapiens (human)]

Gene Symbol CHRNA1
Entrez Gene ID 1134
Full Name cholinergic receptor, nicotinic, alpha 1 (muscle)
Synonyms ACHRA, ACHRD, CHRNA, CMS1A, CMS1B, CMS2A, FCCMS, SCCMS
General protein information
Preferred Names
acetylcholine receptor subunit alpha
Names
acetylcholine receptor subunit alpha
nicotinic cholinergic receptor alpha 1
muscle nicotinic acetylcholine receptor
nicotinic acetylcholine receptor alpha subunit
acetylcholine receptor, nicotinic, alpha 1 (muscle)
cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q31.1

Summary The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha subunits and 1 each of the beta, gamma, and delta subunits. This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2012]. lac of sum
Disorder MIM:

100690

Disorder Html: Myasthenic syndrome, slow-channel congenital, 601462 (3);





The following CHRNA1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19640 NM_001039523 Homo sapiens cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $409
OHu16431 NM_000079 Homo sapiens cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $389

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu19640
Accession Version NM_001039523.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1449bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product acetylcholine receptor subunit alpha isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, CD013889.1 and BC043196.2. This sequence is a reference standard in the RefSeqGene project. On Jun 10, 2008 this sequence version replaced gi:87567782. Summary: The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha subunits and 1 each of the beta, gamma, and delta subunits. This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2012]. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299445.1, CD013889.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC
ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG
CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG
AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT
GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC
ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT
CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC
GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG
TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC
ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC
GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG
CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG
TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA
AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC
ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT
TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA
AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA
GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC
ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG
GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG
CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG
CAAGGATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001034612.1
CDS79..1527
Misc Feature(1)73..75(+)
Misc Feature(2)88..1494(+)
Misc Feature(3)148..846(+)
Misc Feature(4)844..918(+)
Misc Feature(5)865..1491(+)
Misc Feature(6)940..996(+)
Misc Feature(7)1042..1101(+)
Misc Feature(8)1438..1494(+)
Exon (1)1..121
Gene:CHRNA1
Gene Synonym:
Exon (2)122..267
Gene:CHRNA1
Gene Synonym:
Exon (3)268..312
Gene:CHRNA1
Gene Synonym:
Exon (4)313..387
Gene:CHRNA1
Gene Synonym:
Exon (5)388..497
Gene:CHRNA1
Gene Synonym:
Exon (6)498..693
Gene:CHRNA1
Gene Synonym:
Exon (7)694..931
Gene:CHRNA1
Gene Synonym:
Exon (8)932..1155
Gene:CHRNA1
Gene Synonym:
Exon (9)1156..1395
Gene:CHRNA1
Gene Synonym:
Exon (10)1396..2059
Gene:CHRNA1
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, c dbSNP:557281141
33 33 a, g dbSNP:773794266
40 40 c, t dbSNP:765880527
42 42 a, g dbSNP:182459320
55 55 c, g dbSNP:772870911
61 61 c, t dbSNP:375512537
62 62 a, c dbSNP:190007991
70 70 c, t dbSNP:569599752
71 71 a, g dbSNP:138563328
73 73 c, t dbSNP:747529575
74 74 a, t dbSNP:377629045
78 78 a, c dbSNP:747801974
79 79 a, g dbSNP:146464862
80 80 c, t dbSNP:779169597
91 91 c, t dbSNP:567683036
92 92 c, g dbSNP:185544602
94 94 c, g dbSNP:757488589
106 106 g, t dbSNP:752524549
109 109 a, t dbSNP:767232137
110 110 a, g dbSNP:144897150
111 111 a, c dbSNP:569075903
113 113 c, t dbSNP:765824911
117 117 a, c dbSNP:762428267
129 129 c, t dbSNP:751402231
130 130 a, c, g dbSNP:202221890
132 132 c, t dbSNP:756540506
141 141 c, t dbSNP:751313425
142 142 a, g dbSNP:368979094
147 147 a, t dbSNP:548319211
152 152 c, t dbSNP:753460555
154 154 c, t dbSNP:536229089
155 155 a, g dbSNP:777813669
180 180 a, c dbSNP:377254749
183 183 c, t dbSNP:772697720
190 190 a, g dbSNP:569049580
191 191 -, t dbSNP:775368207
196 196 c, t dbSNP:774423842
203 203 g, t dbSNP:770948739
207 207 a, c dbSNP:140247604
214 214 c, t dbSNP:550657234
215 215 a, g dbSNP:149851833
222 222 c, t dbSNP:766106058
235 235 a, g dbSNP:779877755
243 243 a, g dbSNP:758269081
253 253 c, t dbSNP:750329103
255 255 c, g dbSNP:199899006
274 274 a, g dbSNP:775195278
283 283 a, t dbSNP:771882592
285 285 c, t dbSNP:745766303
286 286 a, g dbSNP:778687954
296 296 a, g dbSNP:141121555
298 298 a, g dbSNP:140059448
300 300 -, g dbSNP:199470442
301 301 c, t dbSNP:777443003
302 302 a, g, t dbSNP:147488907
313 313 c, g dbSNP:754725514
317 317 a, g dbSNP:751855931
321 321 g, t dbSNP:766779978
334 334 c, t dbSNP:75536736
335 335 a, g dbSNP:750955953
337 337 a, c dbSNP:750158465
338 338 c, t dbSNP:749874154
344 344 c, g dbSNP:778408913
345 345 c, t dbSNP:776760955
346 346 a, g dbSNP:756593848
355 355 a, g dbSNP:146743352
359 359 c, t dbSNP:760968177
360 360 c, t dbSNP:773954599
361 361 c, t dbSNP:770791722
370 370 c, g, t dbSNP:773262937
371 371 c, t dbSNP:769890214
373 373 a, c dbSNP:373012030
374 374 a, t dbSNP:780811681
384 384 a, t dbSNP:150585253
401 401 a, g dbSNP:769840773
406 406 c, t dbSNP:377205515
408 408 a, c dbSNP:368009217
409 409 a, c dbSNP:775936777
414 414 c, g dbSNP:768240064
423 423 a, t dbSNP:746590795
432 432 a, c, t dbSNP:771749641
433 433 a, g, t dbSNP:199470444
434 434 -, gg dbSNP:144488904
436 436 a, g dbSNP:757698567
438 438 c, g dbSNP:754404989
441 441 a, g dbSNP:777666730
445 445 -, a dbSNP:753250273
445 445 -, a dbSNP:756516966
447 447 c, t dbSNP:575560034
451 451 a, t dbSNP:752761320
454 454 c, t dbSNP:767773452
465 465 g, t dbSNP:759894314
467 467 c, t dbSNP:764809207
472 472 c, t dbSNP:140268343
473 473 a, g dbSNP:376844505
477 477 a, c dbSNP:199555685
483 483 c, t dbSNP:763984762
491 491 a, c dbSNP:760198514
499 499 a, g dbSNP:773971699
500 500 c, t dbSNP:770611155
504 504 c, t dbSNP:749660752
510 510 c, g dbSNP:773509048
517 517 a, g dbSNP:770274911
525 525 a, g dbSNP:143047599
529 529 a, g dbSNP:781376926
537 537 c, g dbSNP:755130911
544 544 c, t dbSNP:747133355
563 563 c, t dbSNP:780391418
564 564 c, g dbSNP:758613275
590 590 a, g dbSNP:753613192
602 602 -, t dbSNP:766019642
603 603 c, g dbSNP:200163630
604 604 a, t dbSNP:560121316
605 605 c, t dbSNP:554434466
606 606 c, t dbSNP:372122272
607 607 a, c, g dbSNP:137852807
609 609 c, g dbSNP:759020321
611 611 a, c, t dbSNP:374915730
612 612 c, t dbSNP:766005384
633 633 a, g dbSNP:762523171
637 637 a, t dbSNP:773556274
641 641 c, g dbSNP:375645058
666 666 c, t dbSNP:762327875
669 669 c, t dbSNP:777255132
670 670 a, g dbSNP:137852801
673 673 g, t dbSNP:766763104
678 678 c, t dbSNP:186421442
679 679 a, c, g dbSNP:137852799
689 689 a, g dbSNP:772271812
692 692 c, t dbSNP:373561258
693 693 a, g dbSNP:370479041
694 694 -, g dbSNP:141198900
699 699 c, t dbSNP:749002411
700 700 a, g dbSNP:145421881
714 714 a, g dbSNP:775673443
719 719 a, g dbSNP:267599011
725 725 c, t dbSNP:772068737
728 728 a, g dbSNP:746009147
730 730 a, g dbSNP:777401754
732 732 c, t dbSNP:534185815
733 733 a, g dbSNP:201147926
739 739 g, t dbSNP:747928217
749 749 a, g dbSNP:76911424
755 755 c, t dbSNP:780981650
757 757 c, t dbSNP:528619587
758 758 a, g, t dbSNP:769337073
766 766 a, t dbSNP:373958739
771 771 c, t dbSNP:757820359
774 774 c, t dbSNP:74533173
775 775 a, g dbSNP:199470445
789 789 a, c dbSNP:764792314
795 795 c, t dbSNP:757027133
796 796 a, c, g dbSNP:148304857
798 798 a, c dbSNP:761217101
800 800 c, t dbSNP:775838764
801 801 c, g, t dbSNP:759534966
802 802 c, t dbSNP:774551857
807 807 c, t dbSNP:771004002
808 808 c, t dbSNP:150638770
810 810 c, g dbSNP:375856493
823 823 a, c, g dbSNP:746851892
828 828 c, t dbSNP:531534298
829 829 a, g dbSNP:200102479
832 832 a, g dbSNP:111856577
838 838 c, t dbSNP:745355427
839 839 a, g, t dbSNP:137852809
840 840 c, t dbSNP:545520806
858 858 a, c, t dbSNP:143252724
861 861 c, t dbSNP:780707128
864 864 c, g, t dbSNP:137852798
867 867 c, t dbSNP:767901131
870 870 c, t dbSNP:760104583
876 876 c, t dbSNP:189442556
882 882 a, g dbSNP:766474459
888 888 a, c, t dbSNP:773397465
890 890 a, c, t dbSNP:483353046
899 899 c, g dbSNP:770040016
903 903 c, t dbSNP:186607397
910 910 g, t dbSNP:137852805
918 918 a, g dbSNP:372014043
929 929 c, g dbSNP:771845966
933 933 a, g dbSNP:148502695
940 940 a, g, t dbSNP:781359883
941 941 c, t dbSNP:755345794
942 942 g, t dbSNP:751905048
946 946 c, g dbSNP:780531882
948 948 a, g dbSNP:758961178
956 956 -, ct dbSNP:749529868
958 958 a, g, t dbSNP:137852803
966 966 a, c, g dbSNP:374093236
972 972 a, g dbSNP:780973898
974 974 c, t dbSNP:137852800
979 979 c, t dbSNP:137852806
993 993 c, t dbSNP:754070026
994 994 a, g dbSNP:764265345
1008 1008 c, t dbSNP:759371067
1013 1013 c, g, t dbSNP:370345788
1014 1014 a, g dbSNP:144020157
1017 1017 c, t dbSNP:780578568
1019 1019 g, t dbSNP:137852802
1023 1023 c, t dbSNP:142633480
1025 1025 c, t dbSNP:762993009
1029 1029 a, c dbSNP:772810584
1034 1034 c, t dbSNP:769372229
1041 1041 a, t dbSNP:747657828
1045 1045 a, g dbSNP:201185358
1048 1048 c, t dbSNP:768256648
1065 1065 c, t dbSNP:373005571
1066 1066 a, g dbSNP:137852804
1072 1072 a, g dbSNP:550013897
1078 1078 a, c dbSNP:199470446
1080 1080 a, c dbSNP:758834482
1088 1088 a, c dbSNP:746404398
1089 1089 g, t dbSNP:779447535
1095 1095 c, t dbSNP:200535960
1096 1096 a, g dbSNP:537285154
1098 1098 c, t dbSNP:371524795
1101 1101 a, c dbSNP:141733086
1108 1108 c, t dbSNP:150110639
1113 1113 a, c, g, t dbSNP:2229957
1115 1115 a, g dbSNP:762938254
1130 1130 a, g dbSNP:773258577
1142 1142 a, g dbSNP:765124080
1145 1145 a, g dbSNP:761377757
1146 1146 g, t dbSNP:776059239
1150 1150 c, t dbSNP:374391312
1156 1156 a, g, t dbSNP:781558501
1159 1159 g, t dbSNP:755304714
1164 1164 c, g, t dbSNP:778575658
1165 1165 c, g dbSNP:757178381
1166 1166 a, g dbSNP:753739833
1168 1168 a, g dbSNP:778616300
1184 1184 c, t dbSNP:764140181
1198 1198 a, g dbSNP:760175390
1199 1199 a, t dbSNP:752263750
1208 1208 a, c dbSNP:767099816
1217 1217 a, g dbSNP:144533405
1220 1220 a, c dbSNP:146863119
1222 1222 a, c dbSNP:536176385
1226 1226 a, t dbSNP:6739001
1227 1227 a, c dbSNP:773564310
1229 1229 a, g dbSNP:770485959
1232 1232 -, a dbSNP:768863116
1236 1236 a, t dbSNP:556317469
1241 1241 c, t dbSNP:146771663
1261 1261 c, g dbSNP:768929776
1283 1283 c, g dbSNP:747224747
1285 1285 c, t dbSNP:778718984
1290 1290 a, c dbSNP:756980389
1291 1291 a, g dbSNP:753734730
1292 1292 a, c, t dbSNP:149292107
1294 1294 a, g dbSNP:199594911
1296 1296 c, t dbSNP:767048858
1304 1304 c, g dbSNP:759182381
1306 1306 c, t dbSNP:751153789
1314 1314 a, c dbSNP:200969433
1315 1315 a, c dbSNP:202090282
1323 1323 c, g, t dbSNP:141280544
1324 1324 a, c, g dbSNP:762411124
1335 1335 a, t dbSNP:776811077
1337 1337 c, t dbSNP:768878508
1342 1342 a, g dbSNP:747162643
1350 1350 c, t dbSNP:775542425
1352 1352 a, g dbSNP:137916282
1353 1353 a, g dbSNP:748978365
1359 1359 a, c, t dbSNP:756006671
1369 1369 a, g dbSNP:376515416
1384 1384 a, g dbSNP:781158981
1386 1386 g, t dbSNP:61737716
1395 1395 c, g, t dbSNP:199545638
1397 1397 c, t dbSNP:200394375
1398 1398 a, g dbSNP:372181669
1400 1400 c, t dbSNP:367751078
1401 1401 -, ggc dbSNP:781704170
1401 1401 a, g, t dbSNP:17838545
1406 1406 a, g dbSNP:765028923
1408 1408 a, t dbSNP:61731797
1410 1410 a, g dbSNP:538109175
1413 1413 a, g dbSNP:757664426
1417 1417 a, g dbSNP:754320308
1419 1419 c, t dbSNP:764451540
1422 1422 a, g dbSNP:761249168
1424 1424 g, t dbSNP:752681554
1431 1431 a, g dbSNP:767659115
1438 1438 a, g dbSNP:754632896
1441 1441 c, t dbSNP:759664482
1446 1446 c, t dbSNP:146899588
1447 1447 a, g dbSNP:151081792
1467 1467 c, g dbSNP:137852808
1470 1470 c, t dbSNP:761586014
1473 1473 c, t dbSNP:776420240
1474 1474 a, g dbSNP:768407867
1476 1476 a, c dbSNP:746995334
1482 1482 a, t dbSNP:779379425
1485 1485 c, t dbSNP:771587252
1486 1486 a, g dbSNP:372104868
1499 1499 a, g, t dbSNP:757027280
1502 1502 c, t dbSNP:111396728
1505 1505 c, t dbSNP:754189390
1512 1512 a, g dbSNP:773619900
1523 1523 a, g dbSNP:778232690
1529 1529 a, c dbSNP:371042588
1539 1539 c, g dbSNP:753228033
1545 1545 c, t dbSNP:767952169
1563 1563 a, c dbSNP:759611404
1564 1564 c, t dbSNP:371795389
1566 1566 a, g dbSNP:766527459
1567 1567 c, g dbSNP:367925483
1573 1573 -, c dbSNP:755507116
1610 1610 c, g dbSNP:762962058
1617 1617 c, t dbSNP:566606143
1627 1627 a, t dbSNP:558788679
1629 1629 a, g dbSNP:376123739
1651 1651 c, t dbSNP:773011739
1694 1694 c, t dbSNP:529078773
1741 1741 g, t dbSNP:190389610
1745 1745 c, t dbSNP:79539026
1792 1792 a, c dbSNP:531470403
1815 1815 a, g dbSNP:564090863
1823 1823 a, g dbSNP:765084448
1843 1843 c, t dbSNP:552179007
1847 1847 a, g dbSNP:527415466
1853 1853 a, g dbSNP:761565416
1862 1862 c, t dbSNP:576344249
1910 1910 c, t dbSNP:768887652
1938 1938 a, g dbSNP:560280191
1944 1944 c, t dbSNP:543652599
1949 1949 g, t dbSNP:184095877
1953 1953 a, c dbSNP:115375214
1956 1956 -, c dbSNP:376801487
1988 1988 a, t dbSNP:542750994
1992 1992 a, g dbSNP:761396980
1993 1993 c, g dbSNP:544906046
2026 2026 a, t dbSNP:574135903

Target ORF information:

RefSeq Version NM_001039523
Organism Homo sapiens (human)
Definition Homo sapiens cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001039523

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC
ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG
CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG
AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT
GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC
ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT
CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC
GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG
TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC
ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC
GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG
CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG
TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA
AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC
ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT
TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA
AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA
GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC
ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG
GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG
CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG
CAAGGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16431
Accession Version NM_000079.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1374bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product acetylcholine receptor subunit alpha isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, Y00762.1 and BC043196.2. On Jun 10, 2008 this sequence version replaced gi:87567780. Summary: The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha subunits and 1 each of the beta, gamma, and delta subunits. This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2012]. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (b), that is missing an internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315312.1, S77094.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG
GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT
CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT
GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT
CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG
AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG
GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG
TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC
ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC
CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG
GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC
GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC
ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC
CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC
CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG
ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG
GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG
TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG
TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC
GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000070.1
CDS79..1452
Misc Feature(1)73..75(+)
Misc Feature(2)88..1419(+)
Misc Feature(3)148..771(+)
Misc Feature(4)790..1416(+)
Exon (1)1..121
Gene:CHRNA1
Gene Synonym:
Exon (2)122..267
Gene:CHRNA1
Gene Synonym:
Exon (3)268..312
Gene:CHRNA1
Gene Synonym:
Exon (4)313..422
Gene:CHRNA1
Gene Synonym:
Exon (5)423..618
Gene:CHRNA1
Gene Synonym:
Exon (6)619..856
Gene:CHRNA1
Gene Synonym:
Exon (7)857..1080
Gene:CHRNA1
Gene Synonym:
Exon (8)1081..1320
Gene:CHRNA1
Gene Synonym:
Exon (9)1321..1984
Gene:CHRNA1
Gene Synonym:
Translation
Position Chain Variation Link
4 4 a, c dbSNP:557281141
33 33 a, g dbSNP:773794266
40 40 c, t dbSNP:765880527
42 42 a, g dbSNP:182459320
55 55 c, g dbSNP:772870911
61 61 c, t dbSNP:375512537
62 62 a, c dbSNP:190007991
70 70 c, t dbSNP:569599752
71 71 a, g dbSNP:138563328
73 73 c, t dbSNP:747529575
74 74 a, t dbSNP:377629045
78 78 a, c dbSNP:747801974
79 79 a, g dbSNP:146464862
80 80 c, t dbSNP:779169597
91 91 c, t dbSNP:567683036
92 92 c, g dbSNP:185544602
94 94 c, g dbSNP:757488589
106 106 g, t dbSNP:752524549
109 109 a, t dbSNP:767232137
110 110 a, g dbSNP:144897150
111 111 a, c dbSNP:569075903
113 113 c, t dbSNP:765824911
117 117 a, c dbSNP:762428267
129 129 c, t dbSNP:751402231
130 130 a, c, g dbSNP:202221890
132 132 c, t dbSNP:756540506
141 141 c, t dbSNP:751313425
142 142 a, g dbSNP:368979094
147 147 a, t dbSNP:548319211
152 152 c, t dbSNP:753460555
154 154 c, t dbSNP:536229089
155 155 a, g dbSNP:777813669
180 180 a, c dbSNP:377254749
183 183 c, t dbSNP:772697720
190 190 a, g dbSNP:569049580
191 191 -, t dbSNP:775368207
196 196 c, t dbSNP:774423842
203 203 g, t dbSNP:770948739
207 207 a, c dbSNP:140247604
214 214 c, t dbSNP:550657234
215 215 a, g dbSNP:149851833
222 222 c, t dbSNP:766106058
235 235 a, g dbSNP:779877755
243 243 a, g dbSNP:758269081
253 253 c, t dbSNP:750329103
255 255 c, g dbSNP:199899006
274 274 a, g dbSNP:775195278
283 283 a, t dbSNP:771882592
285 285 c, t dbSNP:745766303
286 286 a, g dbSNP:778687954
296 296 a, g dbSNP:141121555
298 298 a, g dbSNP:140059448
300 300 -, g dbSNP:199470442
301 301 c, t dbSNP:777443003
302 302 a, g, t dbSNP:147488907
326 326 a, g dbSNP:769840773
331 331 c, t dbSNP:377205515
333 333 a, c dbSNP:368009217
334 334 a, c dbSNP:775936777
339 339 c, g dbSNP:768240064
348 348 a, t dbSNP:746590795
357 357 a, c, t dbSNP:771749641
358 358 a, g, t dbSNP:199470444
359 359 -, gg dbSNP:144488904
361 361 a, g dbSNP:757698567
363 363 c, g dbSNP:754404989
366 366 a, g dbSNP:777666730
370 370 -, a dbSNP:753250273
370 370 -, a dbSNP:756516966
372 372 c, t dbSNP:575560034
376 376 a, t dbSNP:752761320
379 379 c, t dbSNP:767773452
390 390 g, t dbSNP:759894314
392 392 c, t dbSNP:764809207
397 397 c, t dbSNP:140268343
398 398 a, g dbSNP:376844505
402 402 a, c dbSNP:199555685
408 408 c, t dbSNP:763984762
416 416 a, c dbSNP:760198514
424 424 a, g dbSNP:773971699
425 425 c, t dbSNP:770611155
429 429 c, t dbSNP:749660752
435 435 c, g dbSNP:773509048
442 442 a, g dbSNP:770274911
450 450 a, g dbSNP:143047599
454 454 a, g dbSNP:781376926
462 462 c, g dbSNP:755130911
469 469 c, t dbSNP:747133355
488 488 c, t dbSNP:780391418
489 489 c, g dbSNP:758613275
515 515 a, g dbSNP:753613192
527 527 -, t dbSNP:766019642
528 528 c, g dbSNP:200163630
529 529 a, t dbSNP:560121316
530 530 c, t dbSNP:554434466
531 531 c, t dbSNP:372122272
532 532 a, c, g dbSNP:137852807
534 534 c, g dbSNP:759020321
536 536 a, c, t dbSNP:374915730
537 537 c, t dbSNP:766005384
558 558 a, g dbSNP:762523171
562 562 a, t dbSNP:773556274
566 566 c, g dbSNP:375645058
591 591 c, t dbSNP:762327875
594 594 c, t dbSNP:777255132
595 595 a, g dbSNP:137852801
598 598 g, t dbSNP:766763104
603 603 c, t dbSNP:186421442
604 604 a, c, g dbSNP:137852799
614 614 a, g dbSNP:772271812
617 617 c, t dbSNP:373561258
618 618 a, g dbSNP:370479041
619 619 -, g dbSNP:141198900
624 624 c, t dbSNP:749002411
625 625 a, g dbSNP:145421881
639 639 a, g dbSNP:775673443
644 644 a, g dbSNP:267599011
650 650 c, t dbSNP:772068737
653 653 a, g dbSNP:746009147
655 655 a, g dbSNP:777401754
657 657 c, t dbSNP:534185815
658 658 a, g dbSNP:201147926
664 664 g, t dbSNP:747928217
674 674 a, g dbSNP:76911424
680 680 c, t dbSNP:780981650
682 682 c, t dbSNP:528619587
683 683 a, g, t dbSNP:769337073
691 691 a, t dbSNP:373958739
696 696 c, t dbSNP:757820359
699 699 c, t dbSNP:74533173
700 700 a, g dbSNP:199470445
714 714 a, c dbSNP:764792314
720 720 c, t dbSNP:757027133
721 721 a, c, g dbSNP:148304857
723 723 a, c dbSNP:761217101
725 725 c, t dbSNP:775838764
726 726 c, g, t dbSNP:759534966
727 727 c, t dbSNP:774551857
732 732 c, t dbSNP:771004002
733 733 c, t dbSNP:150638770
735 735 c, g dbSNP:375856493
748 748 a, c, g dbSNP:746851892
753 753 c, t dbSNP:531534298
754 754 a, g dbSNP:200102479
757 757 a, g dbSNP:111856577
763 763 c, t dbSNP:745355427
764 764 a, g, t dbSNP:137852809
765 765 c, t dbSNP:545520806
783 783 a, c, t dbSNP:143252724
786 786 c, t dbSNP:780707128
789 789 c, g, t dbSNP:137852798
792 792 c, t dbSNP:767901131
795 795 c, t dbSNP:760104583
801 801 c, t dbSNP:189442556
807 807 a, g dbSNP:766474459
813 813 a, c, t dbSNP:773397465
815 815 a, c, t dbSNP:483353046
824 824 c, g dbSNP:770040016
828 828 c, t dbSNP:186607397
835 835 g, t dbSNP:137852805
843 843 a, g dbSNP:372014043
854 854 c, g dbSNP:771845966
858 858 a, g dbSNP:148502695
865 865 a, g, t dbSNP:781359883
866 866 c, t dbSNP:755345794
867 867 g, t dbSNP:751905048
871 871 c, g dbSNP:780531882
873 873 a, g dbSNP:758961178
881 881 -, ct dbSNP:749529868
883 883 a, g, t dbSNP:137852803
891 891 a, c, g dbSNP:374093236
897 897 a, g dbSNP:780973898
899 899 c, t dbSNP:137852800
904 904 c, t dbSNP:137852806
918 918 c, t dbSNP:754070026
919 919 a, g dbSNP:764265345
933 933 c, t dbSNP:759371067
938 938 c, g, t dbSNP:370345788
939 939 a, g dbSNP:144020157
942 942 c, t dbSNP:780578568
944 944 g, t dbSNP:137852802
948 948 c, t dbSNP:142633480
950 950 c, t dbSNP:762993009
954 954 a, c dbSNP:772810584
959 959 c, t dbSNP:769372229
966 966 a, t dbSNP:747657828
970 970 a, g dbSNP:201185358
973 973 c, t dbSNP:768256648
990 990 c, t dbSNP:373005571
991 991 a, g dbSNP:137852804
997 997 a, g dbSNP:550013897
1003 1003 a, c dbSNP:199470446
1005 1005 a, c dbSNP:758834482
1013 1013 a, c dbSNP:746404398
1014 1014 g, t dbSNP:779447535
1020 1020 c, t dbSNP:200535960
1021 1021 a, g dbSNP:537285154
1023 1023 c, t dbSNP:371524795
1026 1026 a, c dbSNP:141733086
1033 1033 c, t dbSNP:150110639
1038 1038 a, c, g, t dbSNP:2229957
1040 1040 a, g dbSNP:762938254
1055 1055 a, g dbSNP:773258577
1067 1067 a, g dbSNP:765124080
1070 1070 a, g dbSNP:761377757
1071 1071 g, t dbSNP:776059239
1075 1075 c, t dbSNP:374391312
1081 1081 a, g, t dbSNP:781558501
1084 1084 g, t dbSNP:755304714
1089 1089 c, g, t dbSNP:778575658
1090 1090 c, g dbSNP:757178381
1091 1091 a, g dbSNP:753739833
1093 1093 a, g dbSNP:778616300
1109 1109 c, t dbSNP:764140181
1123 1123 a, g dbSNP:760175390
1124 1124 a, t dbSNP:752263750
1133 1133 a, c dbSNP:767099816
1142 1142 a, g dbSNP:144533405
1145 1145 a, c dbSNP:146863119
1147 1147 a, c dbSNP:536176385
1151 1151 a, t dbSNP:6739001
1152 1152 a, c dbSNP:773564310
1154 1154 a, g dbSNP:770485959
1157 1157 -, a dbSNP:768863116
1161 1161 a, t dbSNP:556317469
1166 1166 c, t dbSNP:146771663
1186 1186 c, g dbSNP:768929776
1208 1208 c, g dbSNP:747224747
1210 1210 c, t dbSNP:778718984
1215 1215 a, c dbSNP:756980389
1216 1216 a, g dbSNP:753734730
1217 1217 a, c, t dbSNP:149292107
1219 1219 a, g dbSNP:199594911
1221 1221 c, t dbSNP:767048858
1229 1229 c, g dbSNP:759182381
1231 1231 c, t dbSNP:751153789
1239 1239 a, c dbSNP:200969433
1240 1240 a, c dbSNP:202090282
1248 1248 c, g, t dbSNP:141280544
1249 1249 a, c, g dbSNP:762411124
1260 1260 a, t dbSNP:776811077
1262 1262 c, t dbSNP:768878508
1267 1267 a, g dbSNP:747162643
1275 1275 c, t dbSNP:775542425
1277 1277 a, g dbSNP:137916282
1278 1278 a, g dbSNP:748978365
1284 1284 a, c, t dbSNP:756006671
1294 1294 a, g dbSNP:376515416
1309 1309 a, g dbSNP:781158981
1311 1311 g, t dbSNP:61737716
1320 1320 c, g, t dbSNP:199545638
1322 1322 c, t dbSNP:200394375
1323 1323 a, g dbSNP:372181669
1325 1325 c, t dbSNP:367751078
1326 1326 -, ggc dbSNP:781704170
1326 1326 a, g, t dbSNP:17838545
1331 1331 a, g dbSNP:765028923
1333 1333 a, t dbSNP:61731797
1335 1335 a, g dbSNP:538109175
1338 1338 a, g dbSNP:757664426
1342 1342 a, g dbSNP:754320308
1344 1344 c, t dbSNP:764451540
1347 1347 a, g dbSNP:761249168
1349 1349 g, t dbSNP:752681554
1356 1356 a, g dbSNP:767659115
1363 1363 a, g dbSNP:754632896
1366 1366 c, t dbSNP:759664482
1371 1371 c, t dbSNP:146899588
1372 1372 a, g dbSNP:151081792
1392 1392 c, g dbSNP:137852808
1395 1395 c, t dbSNP:761586014
1398 1398 c, t dbSNP:776420240
1399 1399 a, g dbSNP:768407867
1401 1401 a, c dbSNP:746995334
1407 1407 a, t dbSNP:779379425
1410 1410 c, t dbSNP:771587252
1411 1411 a, g dbSNP:372104868
1424 1424 a, g, t dbSNP:757027280
1427 1427 c, t dbSNP:111396728
1430 1430 c, t dbSNP:754189390
1437 1437 a, g dbSNP:773619900
1448 1448 a, g dbSNP:778232690
1454 1454 a, c dbSNP:371042588
1464 1464 c, g dbSNP:753228033
1470 1470 c, t dbSNP:767952169
1488 1488 a, c dbSNP:759611404
1489 1489 c, t dbSNP:371795389
1491 1491 a, g dbSNP:766527459
1492 1492 c, g dbSNP:367925483
1498 1498 -, c dbSNP:755507116
1535 1535 c, g dbSNP:762962058
1542 1542 c, t dbSNP:566606143
1552 1552 a, t dbSNP:558788679
1554 1554 a, g dbSNP:376123739
1576 1576 c, t dbSNP:773011739
1619 1619 c, t dbSNP:529078773
1666 1666 g, t dbSNP:190389610
1670 1670 c, t dbSNP:79539026
1717 1717 a, c dbSNP:531470403
1740 1740 a, g dbSNP:564090863
1748 1748 a, g dbSNP:765084448
1768 1768 c, t dbSNP:552179007
1772 1772 a, g dbSNP:527415466
1778 1778 a, g dbSNP:761565416
1787 1787 c, t dbSNP:576344249
1835 1835 c, t dbSNP:768887652
1863 1863 a, g dbSNP:560280191
1869 1869 c, t dbSNP:543652599
1874 1874 g, t dbSNP:184095877
1878 1878 a, c dbSNP:115375214
1881 1881 -, c dbSNP:376801487
1913 1913 a, t dbSNP:542750994
1917 1917 a, g dbSNP:761396980
1918 1918 c, g dbSNP:544906046
1951 1951 a, t dbSNP:574135903

Target ORF information:

RefSeq Version NM_000079
Organism Homo sapiens (human)
Definition Homo sapiens cholinergic receptor, nicotinic, alpha 1 (muscle) (CHRNA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000079

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG
GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT
CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT
GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT
CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG
AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG
GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG
TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC
ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC
CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG
GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC
GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC
ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC
CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC
CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG
ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG
GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG
TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG
TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC
GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.