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CHRNA2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CHRNA2
Entrez Gene ID 1135
Full Name cholinergic receptor, nicotinic, alpha 2 (neuronal)
General protein information
Preferred Names
neuronal acetylcholine receptor subunit alpha-2
Names
neuronal acetylcholine receptor subunit alpha-2
acetylcholine receptor, nicotinic, alpha 2 (neuronal)
cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

8

8p21

Summary Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]. lac of sum
Disorder MIM:

118502

Disorder Html: Epilepsy, nocturnal frontal lobe, type 4, 610353 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011544388 XP_011542690 neuronal acetylcholine receptor subunit alpha-2 isoform X1
XM_006716282 XP_006716345 neuronal acetylcholine receptor subunit alpha-2 isoform X1
XM_005273397 XP_005273454 neuronal acetylcholine receptor subunit alpha-2 isoform X2
XM_011544389 XP_011542691 neuronal acetylcholine receptor subunit alpha-2 isoform X3
NM_000742 NP_000733 neuronal acetylcholine receptor subunit alpha-2 isoform 1 precursor
NM_001282455 NP_001269384 neuronal acetylcholine receptor subunit alpha-2 isoform 2 precursor



Homo sapiens (human) CHRNA2 NP_000733.2
Pan troglodytes (chimpanzee) CHRNA2 NP_001029107.1
Macaca mulatta (Rhesus monkey) CHRNA2 XP_001109335.1
Canis lupus familiaris (dog) CHRNA2 XP_005635946.1
Bos taurus (cattle) CHRNA2 NP_001179639.1
Mus musculus (house mouse) Chrna2 NP_659052.1
Rattus norvegicus (Norway rat) Chrna2 NP_596911.1
Gallus gallus (chicken) CHRNA2 NP_990146.1
Drosophila melanogaster (fruit fly) nAcRalpha-96Ab NP_733001.1
Xenopus (Silurana) tropicalis (western clawed frog) chrna2 XP_002941292.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHRNA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu17978
XM_011544388 PREDICTED: Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu17978
XM_006716282 PREDICTED: Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu41986 XM_005273397 PREDICTED: Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu56878 XM_011544389 PREDICTED: Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OHu17978
NM_000742 Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu22359 NM_001282455 Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17978
Clone ID Related Accession (Same CDS sequence) NM_000742 , XM_006716282 , XM_011544388
Accession Version XM_011544388.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neuronal acetylcholine receptor subunit alpha-2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
CTGCCTCCGT TCCTAGCTGG AATGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542690.1
CDS612..2201
Misc Feature(1)780..2177(+)
Misc Feature(2)786..1406(+)
Misc Feature(3)1425..2171(+)
Translation

Target ORF information:

RefSeq Version XM_011544388
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544388

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
CTGCCTCCGT TCCTAGCTGG AATGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17978
Clone ID Related Accession (Same CDS sequence) NM_000742 , XM_006716282 , XM_011544388
Accession Version XM_006716282.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neuronal acetylcholine receptor subunit alpha-2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
CTGCCTCCGT TCCTAGCTGG AATGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006716345.1
CDS521..2110
Misc Feature(1)689..2086(+)
Misc Feature(2)695..1315(+)
Misc Feature(3)1334..2080(+)
Translation

Target ORF information:

RefSeq Version XM_006716282
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006716282

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
CTGCCTCCGT TCCTAGCTGG AATGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu41986
Clone ID Related Accession (Same CDS sequence) XM_005273397
Accession Version XM_005273397.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neuronal acetylcholine receptor subunit alpha-2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005273454.1
CDS179..1291
Misc Feature(1)179..496(+)
Misc Feature(2)515..1261(+)
Translation

Target ORF information:

RefSeq Version XM_005273397
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005273397

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGACCAAGG CCCACCTCTT CTCCACGGGC ACTGTGCACT GGGTGCCCCC GGCCATCTAC 
AAGAGCTCCT GCAGCATCGA CGTCACCTTC TTCCCCTTCG ACCAGCAGAA CTGCAAGATG
AAGTTTGGCT CCTGGACTTA TGACAAGGCC AAGATCGACC TGGAGCAGAT GGAGCAGACT
GTGGACCTGA AGGACTACTG GGAGAGCGGC GAGTGGGCCA TCGTCAATGC CACGGGCACC
TACAACAGCA AGAAGTACGA CTGCTGCGCC GAGATCTACC CCGACGTCAC CTACGCCTTC
GTCATCCGGC GGCTGCCGCT CTTCTACACC ATCAACCTCA TCATCCCCTG CCTGCTCATC
TCCTGCCTCA CTGTGCTGGT CTTCTACCTG CCCTCCGACT GCGGCGAGAA GATCACGCTG
TGCATTTCGG TGCTGCTGTC ACTCACCGTC TTCCTGCTGC TCATCACTGA GATCATCCCG
TCCACCTCGC TGGTCATCCC GCTCATCGGC GAGTACCTGC TGTTCACCAT GATCTTCGTC
ACCCTGTCCA TCGTCATCAC CGTCTTCGTG CTCAATGTGC ACCACCGCTC CCCCAGCACC
CACACCATGC CCCACTGGGT GCGGGGGGCC CTTCTGGGCT GTGTGCCCCG GTGGCTTCTG
ATGAACCGGC CCCCACCACC CGTGGAGCTC TGCCACCCCC TACGCCTGAA GCTCAGCCCC
TCTTATCACT GGCTGGAGAG CAACGTGGAT GCCGAGGAGA GGGAGGTGGT GGTGGAGGAG
GAGGACAGAT GGGCATGTGC AGGTCATGTG GCCCCCTCTG TGGGCACCCT CTGCAGCCAC
GGCCACCTGC ACTCTGGGGC CTCAGGTCCC AAGGCTGAGG CTCTGCTGCA GGAGGGTGAG
CTGCTGCTAT CACCCCACAT GCAGAAGGCA CTGGAAGGTG TGCACTACAT TGCCGACCAC
CTGCGGTCTG AGGATGCTGA CTCTTCGGTG AAGGAGGACT GGAAGTATGT TGCCATGGTC
ATCGACAGGA TCTTCCTCTG GCTGTTTATC ATCGTCTGCT TCCTGGGGAC CATCGGCCTC
TTTCTGCCTC CGTTCCTAGC TGGAATGATC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56878
Clone ID Related Accession (Same CDS sequence) XM_011544389
Accession Version XM_011544389.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 996bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product neuronal acetylcholine receptor subunit alpha-2 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542691.1
CDS1068..2063
Misc Feature(1)1068..1268(+)
Misc Feature(2)1287..2033(+)
Translation

Target ORF information:

RefSeq Version XM_011544389
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544389

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAAGTTTG GCTCCTGGAC TTATGACAAG GCCAAGATCG ACCTGGAGCA GATGGAGCAG 
ACTGTGGACC TGAAGGACTA CTGGGAGAGC GGCGAGTGGG CCATCGTCAA TGCCACGGGC
ACCTACAACA GCAAGAAGTA CGACTGCTGC GCCGAGATCT ACCCCGACGT CACCTACGCC
TTCGTCATCC GGCGGCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
ATCTCCTGCC TCACTGTGCT GGTCTTCTAC CTGCCCTCCG ACTGCGGCGA GAAGATCACG
CTGTGCATTT CGGTGCTGCT GTCACTCACC GTCTTCCTGC TGCTCATCAC TGAGATCATC
CCGTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTGTTCAC CATGATCTTC
GTCACCCTGT CCATCGTCAT CACCGTCTTC GTGCTCAATG TGCACCACCG CTCCCCCAGC
ACCCACACCA TGCCCCACTG GGTGCGGGGG GCCCTTCTGG GCTGTGTGCC CCGGTGGCTT
CTGATGAACC GGCCCCCACC ACCCGTGGAG CTCTGCCACC CCCTACGCCT GAAGCTCAGC
CCCTCTTATC ACTGGCTGGA GAGCAACGTG GATGCCGAGG AGAGGGAGGT GGTGGTGGAG
GAGGAGGACA GATGGGCATG TGCAGGTCAT GTGGCCCCCT CTGTGGGCAC CCTCTGCAGC
CACGGCCACC TGCACTCTGG GGCCTCAGGT CCCAAGGCTG AGGCTCTGCT GCAGGAGGGT
GAGCTGCTGC TATCACCCCA CATGCAGAAG GCACTGGAAG GTGTGCACTA CATTGCCGAC
CACCTGCGGT CTGAGGATGC TGACTCTTCG GTGAAGGAGG ACTGGAAGTA TGTTGCCATG
GTCATCGACA GGATCTTCCT CTGGCTGTTT ATCATCGTCT GCTTCCTGGG GACCATCGGC
CTCTTTCTGC CTCCGTTCCT AGCTGGAATG ATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17978
Clone ID Related Accession (Same CDS sequence) NM_000742 , XM_006716282 , XM_011544388
Accession Version NM_000742.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1590bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neuronal acetylcholine receptor subunit alpha-2 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA407151.1, U62431.1, DA096824.1, CD013901.1, AF311103.5 and AW292180.1. This sequence is a reference standard in the RefSeqGene project. On Sep 11, 2007 this sequence version replaced gi:153792668. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U62431.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
CTGCCTCCGT TCCTAGCTGG AATGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000733.2
CDS610..2199
Misc Feature(1)592..594(+)
Misc Feature(2)778..2175(+)
Misc Feature(3)784..1404(+)
Misc Feature(4)1402..1476(+)
Misc Feature(5)1423..2169(+)
Misc Feature(6)1498..1554(+)
Misc Feature(7)1600..1665(+)
Misc Feature(8)2116..2172(+)
Exon (1)1..473
Gene:CHRNA2
Gene Synonym:
Exon (2)474..682
Gene:CHRNA2
Gene Synonym:
Exon (3)683..903
Gene:CHRNA2
Gene Synonym:
Exon (4)904..948
Gene:CHRNA2
Gene Synonym:
Exon (5)949..1058
Gene:CHRNA2
Gene Synonym:
Exon (6)1059..2073
Gene:CHRNA2
Gene Synonym:
Exon (7)2074..4067
Gene:CHRNA2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000742
Organism Homo sapiens (human)
Definition Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000742

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
TCAGACGTGG TGATTGTGCG CTTTGGACTG TCCATCGCTC AGCTCATCGA TGTGGATGAG
AAGAACCAAA TGATGACCAC CAACGTCTGG CTAAAACAGG AGTGGAGCGA CTACAAACTG
CGCTGGAACC CCACTGATTT TGGCAACATC ACATCTCTCA GGGTCCCTTC TGAGATGATC
TGGATCCCCG ACATTGTTCT CTACAACAAT GCAGATGGGG AGTTTGCAGT GACCCACATG
ACCAAGGCCC ACCTCTTCTC CACGGGCACT GTGCACTGGG TGCCCCCGGC CATCTACAAG
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTCGACC AGCAGAACTG CAAGATGAAG
TTTGGCTCCT GGACTTATGA CAAGGCCAAG ATCGACCTGG AGCAGATGGA GCAGACTGTG
GACCTGAAGG ACTACTGGGA GAGCGGCGAG TGGGCCATCG TCAATGCCAC GGGCACCTAC
AACAGCAAGA AGTACGACTG CTGCGCCGAG ATCTACCCCG ACGTCACCTA CGCCTTCGTC
ATCCGGCGGC TGCCGCTCTT CTACACCATC AACCTCATCA TCCCCTGCCT GCTCATCTCC
TGCCTCACTG TGCTGGTCTT CTACCTGCCC TCCGACTGCG GCGAGAAGAT CACGCTGTGC
ATTTCGGTGC TGCTGTCACT CACCGTCTTC CTGCTGCTCA TCACTGAGAT CATCCCGTCC
ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTGCTGT TCACCATGAT CTTCGTCACC
CTGTCCATCG TCATCACCGT CTTCGTGCTC AATGTGCACC ACCGCTCCCC CAGCACCCAC
ACCATGCCCC ACTGGGTGCG GGGGGCCCTT CTGGGCTGTG TGCCCCGGTG GCTTCTGATG
AACCGGCCCC CACCACCCGT GGAGCTCTGC CACCCCCTAC GCCTGAAGCT CAGCCCCTCT
TATCACTGGC TGGAGAGCAA CGTGGATGCC GAGGAGAGGG AGGTGGTGGT GGAGGAGGAG
GACAGATGGG CATGTGCAGG TCATGTGGCC CCCTCTGTGG GCACCCTCTG CAGCCACGGC
CACCTGCACT CTGGGGCCTC AGGTCCCAAG GCTGAGGCTC TGCTGCAGGA GGGTGAGCTG
CTGCTATCAC CCCACATGCA GAAGGCACTG GAAGGTGTGC ACTACATTGC CGACCACCTG
CGGTCTGAGG ATGCTGACTC TTCGGTGAAG GAGGACTGGA AGTATGTTGC CATGGTCATC
GACAGGATCT TCCTCTGGCT GTTTATCATC GTCTGCTTCC TGGGGACCAT CGGCCTCTTT
CTGCCTCCGT TCCTAGCTGG AATGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22359
Clone ID Related Accession (Same CDS sequence) NM_001282455
Accession Version NM_001282455.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neuronal acetylcholine receptor subunit alpha-2 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311103.5, AK296348.1 and AW292180.1. On Sep 7, 2013 this sequence version replaced gi:530387556. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296348.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153427, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
TCAGACGTGG ATGAGAAGAA CCAAATGATG ACCACCAACG TCTGGCTAAA ACAGGAGTGG
AGCGACTACA AACTGCGCTG GAACCCCACT GATTTTGGCA ACATCACATC TCTCAGGGTC
CCTTCTGAGA TGATCTGGAT CCCCGACATT GTTCTCTACA ACAATGCAGA TGGGGAGTTT
GCAGTGACCC ACATGACCAA GGCCCACCTC TTCTCCACGG GCACTGTGCA CTGGGTGCCC
CCGGCCATCT ACAAGAGCTC CTGCAGCATC GACGTCACCT TCTTCCCCTT CGACCAGCAG
AACTGCAAGA TGAAGTTTGG CTCCTGGACT TATGACAAGG CCAAGATCGA CCTGGAGCAG
ATGGAGCAGA CTGTGGACCT GAAGGACTAC TGGGAGAGCG GCGAGTGGGC CATCGTCAAT
GCCACGGGCA CCTACAACAG CAAGAAGTAC GACTGCTGCG CCGAGATCTA CCCCGACGTC
ACCTACGCCT TCGTCATCCG GCGGCTGCCG CTCTTCTACA CCATCAACCT CATCATCCCC
TGCCTGCTCA TCTCCTGCCT CACTGTGCTG GTCTTCTACC TGCCCTCCGA CTGCGGCGAG
AAGATCACGC TGTGCATTTC GGTGCTGCTG TCACTCACCG TCTTCCTGCT GCTCATCACT
GAGATCATCC CGTCCACCTC GCTGGTCATC CCGCTCATCG GCGAGTACCT GCTGTTCACC
ATGATCTTCG TCACCCTGTC CATCGTCATC ACCGTCTTCG TGCTCAATGT GCACCACCGC
TCCCCCAGCA CCCACACCAT GCCCCACTGG GTGCGGGGGG CCCTTCTGGG CTGTGTGCCC
CGGTGGCTTC TGATGAACCG GCCCCCACCA CCCGTGGAGC TCTGCCACCC CCTACGCCTG
AAGCTCAGCC CCTCTTATCA CTGGCTGGAG AGCAACGTGG ATGCCGAGGA GAGGGAGGTG
GTGGTGGAGG AGGAGGACAG ATGGGCATGT GCAGGTCATG TGGCCCCCTC TGTGGGCACC
CTCTGCAGCC ACGGCCACCT GCACTCTGGG GCCTCAGGTC CCAAGGCTGA GGCTCTGCTG
CAGGAGGGTG AGCTGCTGCT ATCACCCCAC ATGCAGAAGG CACTGGAAGG TGTGCACTAC
ATTGCCGACC ACCTGCGGTC TGAGGATGCT GACTCTTCGG TGAAGGAGGA CTGGAAGTAT
GTTGCCATGG TCATCGACAG GATCTTCCTC TGGCTGTTTA TCATCGTCTG CTTCCTGGGG
ACCATCGGCC TCTTTCTGCC TCCGTTCCTA GCTGGAATGA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269384.1
CDS610..2154
Misc Feature(1)592..594(+)
Misc Feature(2)778..2130(+)
Misc Feature(3)784..1359(+)
Misc Feature(4)1378..2124(+)
Exon (1)1..473
Gene:CHRNA2
Gene Synonym:
Exon (2)474..682
Gene:CHRNA2
Gene Synonym:
Exon (3)683..858
Gene:CHRNA2
Gene Synonym:
Exon (4)859..903
Gene:CHRNA2
Gene Synonym:
Exon (5)904..1013
Gene:CHRNA2
Gene Synonym:
Exon (6)1014..2028
Gene:CHRNA2
Gene Synonym:
Exon (7)2029..4022
Gene:CHRNA2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282455
Organism Homo sapiens (human)
Definition Homo sapiens cholinergic receptor, nicotinic, alpha 2 (neuronal) (CHRNA2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282455

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGCCCCT CCTGTCCTGT GTTCCTGTCC TTCACAAAGC TCAGCCTGTG GTGGCTCCTT 
CTGACCCCAG CAGGTGGAGA GGAAGCTAAG CGCCCACCTC CCAGGGCTCC TGGAGACCCA
CTCTCCTCTC CCAGTCCCAC GGCATTGCCG CAGGGAGGCT CGCATACCGA GACTGAGGAC
CGGCTCTTCA AACACCTCTT CCGGGGCTAC AACCGCTGGG CGCGCCCGGT GCCCAACACT
TCAGACGTGG ATGAGAAGAA CCAAATGATG ACCACCAACG TCTGGCTAAA ACAGGAGTGG
AGCGACTACA AACTGCGCTG GAACCCCACT GATTTTGGCA ACATCACATC TCTCAGGGTC
CCTTCTGAGA TGATCTGGAT CCCCGACATT GTTCTCTACA ACAATGCAGA TGGGGAGTTT
GCAGTGACCC ACATGACCAA GGCCCACCTC TTCTCCACGG GCACTGTGCA CTGGGTGCCC
CCGGCCATCT ACAAGAGCTC CTGCAGCATC GACGTCACCT TCTTCCCCTT CGACCAGCAG
AACTGCAAGA TGAAGTTTGG CTCCTGGACT TATGACAAGG CCAAGATCGA CCTGGAGCAG
ATGGAGCAGA CTGTGGACCT GAAGGACTAC TGGGAGAGCG GCGAGTGGGC CATCGTCAAT
GCCACGGGCA CCTACAACAG CAAGAAGTAC GACTGCTGCG CCGAGATCTA CCCCGACGTC
ACCTACGCCT TCGTCATCCG GCGGCTGCCG CTCTTCTACA CCATCAACCT CATCATCCCC
TGCCTGCTCA TCTCCTGCCT CACTGTGCTG GTCTTCTACC TGCCCTCCGA CTGCGGCGAG
AAGATCACGC TGTGCATTTC GGTGCTGCTG TCACTCACCG TCTTCCTGCT GCTCATCACT
GAGATCATCC CGTCCACCTC GCTGGTCATC CCGCTCATCG GCGAGTACCT GCTGTTCACC
ATGATCTTCG TCACCCTGTC CATCGTCATC ACCGTCTTCG TGCTCAATGT GCACCACCGC
TCCCCCAGCA CCCACACCAT GCCCCACTGG GTGCGGGGGG CCCTTCTGGG CTGTGTGCCC
CGGTGGCTTC TGATGAACCG GCCCCCACCA CCCGTGGAGC TCTGCCACCC CCTACGCCTG
AAGCTCAGCC CCTCTTATCA CTGGCTGGAG AGCAACGTGG ATGCCGAGGA GAGGGAGGTG
GTGGTGGAGG AGGAGGACAG ATGGGCATGT GCAGGTCATG TGGCCCCCTC TGTGGGCACC
CTCTGCAGCC ACGGCCACCT GCACTCTGGG GCCTCAGGTC CCAAGGCTGA GGCTCTGCTG
CAGGAGGGTG AGCTGCTGCT ATCACCCCAC ATGCAGAAGG CACTGGAAGG TGTGCACTAC
ATTGCCGACC ACCTGCGGTC TGAGGATGCT GACTCTTCGG TGAAGGAGGA CTGGAAGTAT
GTTGCCATGG TCATCGACAG GATCTTCCTC TGGCTGTTTA TCATCGTCTG CTTCCTGGGG
ACCATCGGCC TCTTTCTGCC TCCGTTCCTA GCTGGAATGA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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