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CHRNE cholinergic receptor, nicotinic, epsilon (muscle) [Homo sapiens (human)]

Gene Symbol CHRNE
Entrez Gene ID 1145
Full Name cholinergic receptor, nicotinic, epsilon (muscle)
Synonyms ACHRE, CMS1D, CMS1E, CMS2A, CMS4A, CMS4B, CMS4C, FCCMS, SCCMS
General protein information
Preferred Names
acetylcholine receptor subunit epsilon
Names
acetylcholine receptor subunit epsilon
AchR epsilon subunit
acetylcholine receptor, nicotinic, epsilon (muscle)
cholinergic receptor, nicotinic, epsilon polypeptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17p13.2

Summary Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

100725

Disorder Html: Myasthenic syndrome, slow-channel congenital, 601462 (3);





The following CHRNE gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNE gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu56906 XM_011523631 PREDICTED: Homo sapiens cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu20760 NM_000080 Homo sapiens cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu56906
Accession Version XM_011523631.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product acetylcholine receptor subunit epsilon isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010718.17) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGGTTCCTTA TTTTCGTCAT GGTGGTCGCC ACGCTCATTG
TCATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011521933.1
CDS66..911
Misc Feature(1)135..785(+)
Translation
Position Chain Variation Link
19 19 c, t dbSNP:751857141
22 22 a, c dbSNP:766690656
25 25 c, t dbSNP:763267064
32 32 c, g dbSNP:773622057
39 39 a, g dbSNP:765722526
57 57 c, g, t dbSNP:373612116
58 58 a, g dbSNP:77481135
65 65 a, g dbSNP:746498663
67 67 c, t dbSNP:775162673
69 69 a, g dbSNP:563301083
71 71 a, g dbSNP:202198207
72 72 a, g dbSNP:201118080
79 79 c, t dbSNP:778780202
80 80 a, g dbSNP:756933520
81 81 c, g, t dbSNP:746521766
87 87 a, g dbSNP:755161498
88 88 c, t dbSNP:199807050
89 89 c, g dbSNP:147811172
90 90 -, c dbSNP:766471461
100 100 g, t dbSNP:758635056
102 102 a, g dbSNP:372635387
106 106 a, t dbSNP:765641852
110 110 c, t dbSNP:34563587
111 111 a, c, g dbSNP:764552360
116 116 c, g dbSNP:758923058
118 118 c, g, t dbSNP:4790235
122 122 a, g dbSNP:750099799
131 131 c, t dbSNP:55770091
141 141 c, t dbSNP:773131873
142 142 a, g dbSNP:188661284
149 149 g, t dbSNP:748103983
155 155 c, t dbSNP:528833386
157 157 c, t dbSNP:772240882
168 168 c, t dbSNP:144169073
171 171 a, g dbSNP:779257808
176 176 a, g dbSNP:757452910
183 183 c, t dbSNP:754001856
187 187 c, t dbSNP:778114846
192 192 c, g dbSNP:756429518
195 195 -, g dbSNP:762368691
205 205 a, t dbSNP:753272609
216 216 a, g dbSNP:759708816
219 219 a, c dbSNP:767890748
220 220 a, g dbSNP:759127314
222 222 c, t dbSNP:751027608
239 239 a, g dbSNP:373275223
240 240 a, g dbSNP:762711228
243 243 c, t dbSNP:370840663
246 246 a, t dbSNP:765180335
248 248 c, t dbSNP:761677000
252 252 -, ctcac dbSNP:776927709
252 252 c, g dbSNP:776667913
254 254 a, g dbSNP:768469610
266 266 g, t dbSNP:752050652
267 267 -, gag dbSNP:769000275
267 267 a, c, g dbSNP:149766604
275 275 c, t dbSNP:750125430
278 278 c, t dbSNP:764949297
280 280 c, g, t dbSNP:776272606
281 281 c, t dbSNP:763887744
285 285 a, g dbSNP:760534437
288 288 c, t dbSNP:193919341
290 290 a, g dbSNP:757046501
293 293 a, g, t dbSNP:771019646
299 299 c, t dbSNP:749350232
302 302 c, t dbSNP:777458923
315 315 c, g, t dbSNP:121909513
320 320 c, g dbSNP:752486312
323 323 c, t dbSNP:368022587
335 335 c, g dbSNP:759482700
343 343 c, g dbSNP:751436557
346 346 a, g dbSNP:765267109
349 349 c, t dbSNP:761907094
355 355 c, t dbSNP:776769810
356 356 c, t dbSNP:768916373
358 358 c, t dbSNP:28929768
359 359 c, g dbSNP:776006666
376 376 c, t dbSNP:772495418
378 378 a, g dbSNP:746407563
390 390 a, g dbSNP:779426416
396 396 c, g dbSNP:770394898
402 402 a, g dbSNP:748799485
407 407 -, c dbSNP:770675734
412 412 c, t dbSNP:760043533
414 414 g, t dbSNP:199708479
415 415 a, g dbSNP:766595838
419 419 c, t dbSNP:763520880
420 420 c, g dbSNP:773553639
421 421 a, g dbSNP:769318953
422 422 a, g dbSNP:747638974
424 424 c, t dbSNP:771356927
425 425 c, t dbSNP:140042356
427 427 g, t dbSNP:746582621
428 428 a, t dbSNP:779901038
440 440 c, t dbSNP:758084108
441 441 g, t dbSNP:200515887
442 442 c, t dbSNP:571215814
446 446 c, t dbSNP:756301699
451 451 c, t dbSNP:752818051
452 452 c, t dbSNP:767908282
455 455 c, t dbSNP:749659243
464 464 a, c, t dbSNP:751665448
465 465 a, g dbSNP:183983159
467 467 c, t dbSNP:766798452
469 469 c, t dbSNP:763181728
471 471 a, g dbSNP:773822199
475 475 a, c, t dbSNP:146089157
480 480 c, g dbSNP:776129600
486 486 a, c dbSNP:483353045
487 487 c, t dbSNP:121909512
490 490 c, t dbSNP:760402668
503 503 c, t dbSNP:776429372
504 504 a, g, t dbSNP:771950733
506 506 a, c, t dbSNP:779058823
513 513 c, g dbSNP:770978243
515 515 a, g dbSNP:778196743
520 520 a, t dbSNP:142901746
522 522 a, t dbSNP:535287601
527 527 c, t dbSNP:781427923
530 530 c, t dbSNP:139625105
533 533 c, t dbSNP:751830305
534 534 c, t dbSNP:368846437
539 539 c, t dbSNP:150868720
543 543 a, c dbSNP:750784391
544 544 a, c dbSNP:765794362
545 545 a, c, g dbSNP:754300536
553 553 c, g, t dbSNP:121909516
564 564 c, t dbSNP:775247070
565 565 g, t dbSNP:121909514
571 571 a, g, t dbSNP:148370803
574 574 c, g dbSNP:375652301
575 575 a, g dbSNP:749454549
581 581 c, t dbSNP:143410438
584 584 c, t dbSNP:33970119
594 594 -, gag dbSNP:779816027
600 600 a, c, g dbSNP:748624980
601 601 c, t dbSNP:781710440
605 605 c, t dbSNP:754577734
606 606 a, g dbSNP:751113232
608 608 c, g dbSNP:766195083
616 616 a, g dbSNP:758142501
620 620 c, t dbSNP:137952354
621 621 a, g dbSNP:764960076
623 623 c, t dbSNP:761643342
634 634 a, g dbSNP:753756406
646 646 c, t dbSNP:146931108
648 648 a, c, g dbSNP:774425374
649 649 a, c dbSNP:376496751
653 653 a, g dbSNP:763114306
658 658 c, t dbSNP:773435892
669 669 a, t dbSNP:760565441
679 679 -, gggccat dbSNP:753828284
687 687 a, g dbSNP:772616743
694 694 g, t dbSNP:746300839
697 697 a, c dbSNP:773916039
698 698 a, g dbSNP:770306808
708 708 c, t dbSNP:748961544
711 711 c, t dbSNP:577641512
714 714 -, gcc dbSNP:764709459
715 715 a, g dbSNP:755943507
716 716 c, t dbSNP:564044217
717 717 c, t dbSNP:781166271
718 718 a, c dbSNP:376001778
719 719 c, g dbSNP:751388919
720 720 a, g dbSNP:765394012
726 726 a, g dbSNP:757240025
730 730 c, t dbSNP:754148818
735 735 a, g dbSNP:531123327
736 736 a, g dbSNP:141183965
739 739 c, t dbSNP:776008550
745 745 a, c dbSNP:767704727
760 760 a, t dbSNP:760118625
764 764 a, g dbSNP:375146171
769 769 c, t dbSNP:770523947
770 770 c, g dbSNP:748765369
771 771 a, c dbSNP:145522662
774 774 c, t dbSNP:769424853
775 775 a, g, t dbSNP:201434993
779 779 a, g dbSNP:768299579
780 780 a, c dbSNP:746993242
786 786 c, t dbSNP:28999110
791 791 c, t dbSNP:150133823
810 810 a, g dbSNP:757421529
814 814 a, c dbSNP:753951775
818 818 c, t dbSNP:777939796
821 821 a, g dbSNP:756407780
827 827 c, t dbSNP:752926340
830 830 a, g dbSNP:767945817
833 833 c, t dbSNP:372509633
834 834 c, t dbSNP:140846401
842 842 g, t dbSNP:752148595
845 845 c, t dbSNP:766792066
846 846 g, t dbSNP:762509943
848 848 c, g dbSNP:772723622
849 849 c, t dbSNP:769228953
854 854 c, g dbSNP:761487611
859 859 -, c dbSNP:756675414
859 859 c, t dbSNP:759226183
862 862 c, t dbSNP:201463598
863 863 c, g dbSNP:746772722
865 865 a, g dbSNP:780094155
867 867 a, g dbSNP:772145808
872 872 c, t dbSNP:777566303
880 880 c, t dbSNP:769436188
892 892 c, t dbSNP:748155593
893 893 a, g dbSNP:780968512
895 895 c, t dbSNP:758560981
897 897 c, t dbSNP:146533655
898 898 a, g dbSNP:144207552
901 901 c, t dbSNP:757563966
916 916 c, t dbSNP:56377005
919 919 a, c dbSNP:532669011
937 937 a, c dbSNP:760928143
939 939 a, g dbSNP:747017964
941 941 a, c, t dbSNP:121909515
942 942 a, g dbSNP:760022829
944 944 a, t dbSNP:773865411
945 945 c, t dbSNP:770380033
946 946 c, g, t dbSNP:772819662
949 949 c, t dbSNP:769677038
952 952 c, t dbSNP:747880376
954 954 c, t dbSNP:781253835
956 956 a, c dbSNP:144747573

Target ORF information:

RefSeq Version XM_011523631
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523631

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGGTTCCTTA TTTTCGTCAT GGTGGTCGCC ACGCTCATTG
TCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20760
Accession Version NM_000080.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product acetylcholine receptor subunit epsilon precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X66403.1 and AC233723.2. This sequence is a reference standard in the RefSeqGene project. On Apr 20, 2010 this sequence version replaced gi:38327653. Summary: Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X66403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000071.1
CDS12..1493
Misc Feature(1)72..1436(+)
Misc Feature(2)81..731(+)
Misc Feature(3)729..803(+)
Misc Feature(4)750..1433(+)
Misc Feature(5)828..884(+)
Misc Feature(6)930..995(+)
Misc Feature(7)1380..1451(+)
Exon (1)1..57
Gene:CHRNE
Gene Synonym:
Exon (2)58..200
Gene:CHRNE
Gene Synonym:
Exon (3)201..245
Gene:CHRNE
Gene Synonym:
Exon (4)246..355
Gene:CHRNE
Gene Synonym:
Exon (5)356..511
Gene:CHRNE
Gene Synonym:
Exon (6)512..612
Gene:CHRNE
Gene Synonym:
Exon (7)613..813
Gene:CHRNE
Gene Synonym:
Exon (8)814..928
Gene:CHRNE
Gene Synonym:
Exon (9)929..1043
Gene:CHRNE
Gene Synonym:
Exon (10)1044..1230
Gene:CHRNE
Gene Synonym:
Exon (11)1231..1337
Gene:CHRNE
Gene Synonym:
Exon (12)1338..2460
Gene:CHRNE
Gene Synonym:
Translation
Position Chain Variation Link
3 3 c, g, t dbSNP:373612116
4 4 a, g dbSNP:77481135
11 11 a, g dbSNP:746498663
13 13 c, t dbSNP:775162673
15 15 a, g dbSNP:563301083
17 17 a, g dbSNP:202198207
18 18 a, g dbSNP:201118080
25 25 c, t dbSNP:778780202
26 26 a, g dbSNP:756933520
27 27 c, g, t dbSNP:746521766
33 33 a, g dbSNP:755161498
34 34 c, t dbSNP:199807050
35 35 c, g dbSNP:147811172
36 36 -, c dbSNP:766471461
46 46 g, t dbSNP:758635056
48 48 a, g dbSNP:372635387
52 52 a, t dbSNP:765641852
56 56 c, t dbSNP:34563587
57 57 a, c, g dbSNP:764552360
62 62 c, g dbSNP:758923058
64 64 c, g, t dbSNP:4790235
68 68 a, g dbSNP:750099799
77 77 c, t dbSNP:55770091
87 87 c, t dbSNP:773131873
88 88 a, g dbSNP:188661284
95 95 g, t dbSNP:748103983
101 101 c, t dbSNP:528833386
103 103 c, t dbSNP:772240882
114 114 c, t dbSNP:144169073
117 117 a, g dbSNP:779257808
122 122 a, g dbSNP:757452910
129 129 c, t dbSNP:754001856
133 133 c, t dbSNP:778114846
138 138 c, g dbSNP:756429518
141 141 -, g dbSNP:762368691
151 151 a, t dbSNP:753272609
162 162 a, g dbSNP:759708816
165 165 a, c dbSNP:767890748
166 166 a, g dbSNP:759127314
168 168 c, t dbSNP:751027608
185 185 a, g dbSNP:373275223
186 186 a, g dbSNP:762711228
189 189 c, t dbSNP:370840663
192 192 a, t dbSNP:765180335
194 194 c, t dbSNP:761677000
198 198 -, ctcac dbSNP:776927709
198 198 c, g dbSNP:776667913
200 200 a, g dbSNP:768469610
212 212 g, t dbSNP:752050652
213 213 -, gag dbSNP:769000275
213 213 a, c, g dbSNP:149766604
221 221 c, t dbSNP:750125430
224 224 c, t dbSNP:764949297
226 226 c, g, t dbSNP:776272606
227 227 c, t dbSNP:763887744
231 231 a, g dbSNP:760534437
234 234 c, t dbSNP:193919341
236 236 a, g dbSNP:757046501
239 239 a, g, t dbSNP:771019646
245 245 c, t dbSNP:749350232
248 248 c, t dbSNP:777458923
261 261 c, g, t dbSNP:121909513
266 266 c, g dbSNP:752486312
269 269 c, t dbSNP:368022587
281 281 c, g dbSNP:759482700
289 289 c, g dbSNP:751436557
292 292 a, g dbSNP:765267109
295 295 c, t dbSNP:761907094
301 301 c, t dbSNP:776769810
302 302 c, t dbSNP:768916373
304 304 c, t dbSNP:28929768
305 305 c, g dbSNP:776006666
322 322 c, t dbSNP:772495418
324 324 a, g dbSNP:746407563
336 336 a, g dbSNP:779426416
342 342 c, g dbSNP:770394898
348 348 a, g dbSNP:748799485
353 353 -, c dbSNP:770675734
358 358 c, t dbSNP:760043533
360 360 g, t dbSNP:199708479
361 361 a, g dbSNP:766595838
365 365 c, t dbSNP:763520880
366 366 c, g dbSNP:773553639
367 367 a, g dbSNP:769318953
368 368 a, g dbSNP:747638974
370 370 c, t dbSNP:771356927
371 371 c, t dbSNP:140042356
373 373 g, t dbSNP:746582621
374 374 a, t dbSNP:779901038
386 386 c, t dbSNP:758084108
387 387 g, t dbSNP:200515887
388 388 c, t dbSNP:571215814
392 392 c, t dbSNP:756301699
397 397 c, t dbSNP:752818051
398 398 c, t dbSNP:767908282
401 401 c, t dbSNP:749659243
410 410 a, c, t dbSNP:751665448
411 411 a, g dbSNP:183983159
413 413 c, t dbSNP:766798452
415 415 c, t dbSNP:763181728
417 417 a, g dbSNP:773822199
421 421 a, c, t dbSNP:146089157
426 426 c, g dbSNP:776129600
432 432 a, c dbSNP:483353045
433 433 c, t dbSNP:121909512
436 436 c, t dbSNP:760402668
449 449 c, t dbSNP:776429372
450 450 a, g, t dbSNP:771950733
452 452 a, c, t dbSNP:779058823
459 459 c, g dbSNP:770978243
461 461 a, g dbSNP:778196743
466 466 a, t dbSNP:142901746
468 468 a, t dbSNP:535287601
473 473 c, t dbSNP:781427923
476 476 c, t dbSNP:139625105
479 479 c, t dbSNP:751830305
480 480 c, t dbSNP:368846437
485 485 c, t dbSNP:150868720
489 489 a, c dbSNP:750784391
490 490 a, c dbSNP:765794362
491 491 a, c, g dbSNP:754300536
499 499 c, g, t dbSNP:121909516
510 510 c, t dbSNP:775247070
511 511 g, t dbSNP:121909514
517 517 a, g, t dbSNP:148370803
520 520 c, g dbSNP:375652301
521 521 a, g dbSNP:749454549
527 527 c, t dbSNP:143410438
530 530 c, t dbSNP:33970119
540 540 -, gag dbSNP:779816027
546 546 a, c, g dbSNP:748624980
547 547 c, t dbSNP:781710440
551 551 c, t dbSNP:754577734
552 552 a, g dbSNP:751113232
554 554 c, g dbSNP:766195083
562 562 a, g dbSNP:758142501
566 566 c, t dbSNP:137952354
567 567 a, g dbSNP:764960076
569 569 c, t dbSNP:761643342
580 580 a, g dbSNP:753756406
592 592 c, t dbSNP:146931108
594 594 a, c, g dbSNP:774425374
595 595 a, c dbSNP:376496751
599 599 a, g dbSNP:763114306
604 604 c, t dbSNP:773435892
615 615 a, t dbSNP:760565441
625 625 -, gggccat dbSNP:753828284
633 633 a, g dbSNP:772616743
640 640 g, t dbSNP:746300839
643 643 a, c dbSNP:773916039
644 644 a, g dbSNP:770306808
654 654 c, t dbSNP:748961544
657 657 c, t dbSNP:577641512
660 660 -, gcc dbSNP:764709459
661 661 a, g dbSNP:755943507
662 662 c, t dbSNP:564044217
663 663 c, t dbSNP:781166271
664 664 a, c dbSNP:376001778
665 665 c, g dbSNP:751388919
666 666 a, g dbSNP:765394012
672 672 a, g dbSNP:757240025
676 676 c, t dbSNP:754148818
681 681 a, g dbSNP:531123327
682 682 a, g dbSNP:141183965
685 685 c, t dbSNP:776008550
691 691 a, c dbSNP:767704727
706 706 a, t dbSNP:760118625
710 710 a, g dbSNP:375146171
715 715 c, t dbSNP:770523947
716 716 c, g dbSNP:748765369
717 717 a, c dbSNP:145522662
720 720 c, t dbSNP:769424853
721 721 a, g, t dbSNP:201434993
725 725 a, g dbSNP:768299579
726 726 a, c dbSNP:746993242
732 732 c, t dbSNP:28999110
737 737 c, t dbSNP:150133823
756 756 a, g dbSNP:757421529
760 760 a, c dbSNP:753951775
764 764 c, t dbSNP:777939796
767 767 a, g dbSNP:756407780
773 773 c, t dbSNP:752926340
776 776 a, g dbSNP:767945817
779 779 c, t dbSNP:372509633
780 780 c, t dbSNP:140846401
788 788 g, t dbSNP:752148595
791 791 c, t dbSNP:766792066
792 792 g, t dbSNP:762509943
794 794 c, g dbSNP:772723622
795 795 c, t dbSNP:769228953
800 800 c, g dbSNP:761487611
805 805 -, c dbSNP:756675414
805 805 c, t dbSNP:759226183
808 808 c, t dbSNP:201463598
809 809 c, g dbSNP:746772722
811 811 a, g dbSNP:780094155
813 813 a, g dbSNP:772145808
815 815 c, t dbSNP:775149431
829 829 c, g, t dbSNP:745821689
831 831 a, g dbSNP:774407731
832 832 c, t dbSNP:201841042
833 833 c, g, t dbSNP:781536510
838 838 c, g dbSNP:570266272
842 842 c, t dbSNP:755170993
852 852 c, t dbSNP:747189862
857 857 c, g dbSNP:780429979
858 858 a, c, t dbSNP:773929089
859 859 a, g dbSNP:750981766
861 861 a, c dbSNP:121909510
863 863 c, t dbSNP:770586668
867 867 g, t dbSNP:756844933
872 872 g, t dbSNP:151006465
876 876 c, t dbSNP:121909511
878 878 c, t dbSNP:763590279
880 880 c, t dbSNP:760274806
881 881 c, t dbSNP:775157123
894 894 c, t dbSNP:61735425
899 899 c, g dbSNP:759372801
900 900 a, t dbSNP:774497563
901 901 c, t dbSNP:770980262
903 903 -, tct dbSNP:398122830
907 907 c, t dbSNP:142735836
910 910 a, g dbSNP:776585678
911 911 a, c, t dbSNP:747224472
912 912 a, g dbSNP:140023380
916 916 c, g, t dbSNP:370019023
917 917 a, g dbSNP:374156332
923 923 c, g dbSNP:779186524
933 933 c, t dbSNP:777566303
941 941 c, t dbSNP:769436188
953 953 c, t dbSNP:748155593
954 954 a, g dbSNP:780968512
956 956 c, t dbSNP:758560981
958 958 c, t dbSNP:146533655
959 959 a, g dbSNP:144207552
962 962 c, t dbSNP:757563966
977 977 c, t dbSNP:56377005
980 980 a, c dbSNP:532669011
998 998 a, c dbSNP:760928143
1000 1000 a, g dbSNP:747017964
1002 1002 a, c, t dbSNP:121909515
1003 1003 a, g dbSNP:760022829
1005 1005 a, t dbSNP:773865411
1006 1006 c, t dbSNP:770380033
1007 1007 c, g, t dbSNP:772819662
1010 1010 c, t dbSNP:769677038
1013 1013 c, t dbSNP:747880376
1015 1015 c, t dbSNP:781253835
1017 1017 a, c dbSNP:144747573
1019 1019 -, cacccac dbSNP:757396750
1019 1019 c, g dbSNP:768479748
1023 1023 a, c dbSNP:200035803
1028 1028 c, g dbSNP:114454383
1030 1030 a, c, g, t dbSNP:139922367
1033 1033 a, g dbSNP:369641727
1035 1035 c, t dbSNP:778130828
1042 1042 a, g dbSNP:756571784
1043 1043 c, t dbSNP:144047383
1046 1046 c, t dbSNP:752110136
1051 1051 a, t dbSNP:766856649
1053 1053 a, g dbSNP:757968612
1059 1059 c, g dbSNP:749936691
1066 1066 g, t dbSNP:764744220
1067 1067 a, c, t dbSNP:558633663
1071 1071 c, t dbSNP:763847983
1072 1072 c, t dbSNP:760538111
1076 1076 c, t dbSNP:775638720
1082 1082 a, g dbSNP:772143528
1085 1085 a, g dbSNP:745744138
1086 1086 c, t dbSNP:773187552
1087 1087 a, c, t dbSNP:192195094
1090 1090 -, gcc dbSNP:752226476
1092 1092 a, g dbSNP:781453095
1094 1094 g, t dbSNP:755391448
1101 1101 c, t dbSNP:747368522
1104 1104 a, g dbSNP:780327519
1106 1106 a, c, t dbSNP:750889051
1109 1109 c, t dbSNP:778313885
1111 1111 c, g, t dbSNP:753495337
1113 1113 c, g dbSNP:763837110
1114 1114 c, t dbSNP:371919434
1115 1115 a, c, t dbSNP:767374241
1116 1116 c, t dbSNP:759577243
1119 1119 a, g dbSNP:774315307
1122 1122 c, t dbSNP:769811534
1130 1130 c, g, t dbSNP:372862723
1132 1132 a, c dbSNP:768869197
1137 1137 c, g dbSNP:553626728
1148 1148 c, g, t dbSNP:368940455
1149 1149 c, t dbSNP:376093621
1152 1152 a, g dbSNP:547989166
1153 1153 a, c, t dbSNP:756786529
1162 1162 g, t dbSNP:753397420
1163 1163 g, t dbSNP:777363943
1166 1166 a, g dbSNP:755854654
1167 1167 c, t dbSNP:752358041
1173 1173 -, aag dbSNP:763532183
1175 1175 a, g dbSNP:767462219
1179 1179 c, t dbSNP:140763858
1180 1180 c, g dbSNP:568952207
1183 1183 g, t dbSNP:138546068
1184 1184 a, c, t dbSNP:146921234
1187 1187 a, g dbSNP:768670572
1188 1188 c, g dbSNP:760915482
1191 1191 a, g, t dbSNP:772486503
1194 1194 g, t dbSNP:755802710
1202 1202 g, t dbSNP:779084533
1203 1203 c, t dbSNP:771486772
1207 1207 a, g dbSNP:749710960
1212 1212 c, t dbSNP:551856227
1213 1213 a, g dbSNP:367705187
1217 1217 a, g dbSNP:768053895
1222 1222 c, t dbSNP:747853975
1224 1224 c, t dbSNP:780976389
1227 1227 a, g dbSNP:762165400
1228 1228 c, t dbSNP:532120320
1229 1229 c, g dbSNP:751373916
1234 1234 c, g dbSNP:778624148
1235 1235 -, c dbSNP:778012442
1237 1237 g, t dbSNP:757212314
1239 1239 c, t dbSNP:753739289
1252 1252 c, g dbSNP:781373404
1253 1253 a, c, t dbSNP:370770111
1254 1254 a, g dbSNP:766507655
1255 1255 c, t dbSNP:763199413
1256 1256 c, t dbSNP:202051373
1260 1260 a, g dbSNP:765647575
1261 1261 a, c, g, t dbSNP:768258598
1265 1265 -, ccc dbSNP:769945146
1266 1266 a, g dbSNP:754975887
1268 1268 g, t dbSNP:775001449
1272 1272 c, t dbSNP:369709668
1273 1273 a, g dbSNP:144952259
1276 1276 a, g dbSNP:778714163
1278 1278 a, t dbSNP:372515997
1280 1280 g, t dbSNP:749273134
1286 1286 g, t dbSNP:777560468
1287 1287 a, g dbSNP:755154052
1289 1289 c, t dbSNP:751593666
1290 1290 a, g dbSNP:780122731
1293 1293 a, t dbSNP:758716291
1295 1295 c, t dbSNP:750634265
1300 1300 c, g, t dbSNP:200343520
1302 1302 c, g, t dbSNP:121909517
1303 1303 c, t dbSNP:761189555
1304 1304 c, t dbSNP:33978919
1307 1307 a, c, g dbSNP:759230979
1310 1310 c, t dbSNP:773970037
1313 1313 g, t dbSNP:770772272
1316 1316 a, g dbSNP:55681486
1325 1325 c, g dbSNP:772995680
1328 1328 c, t dbSNP:769630764
1329 1329 a, g dbSNP:748069648
1332 1332 a, g dbSNP:375221757
1334 1334 c, t dbSNP:758517310
1336 1336 -, a dbSNP:755420360
1338 1338 -, g dbSNP:763258280
1343 1343 c, g dbSNP:376975376
1345 1345 c, t dbSNP:756483707
1346 1346 c, t dbSNP:753202734
1347 1347 g, t dbSNP:768008177
1349 1349 c, g dbSNP:755412049
1353 1353 c, g dbSNP:762582525
1355 1355 a, c, g dbSNP:143098859
1356 1356 a, c, t dbSNP:149083639
1361 1361 a, g dbSNP:761651772
1364 1364 -, g dbSNP:773526895
1366 1366 -, a dbSNP:770107793
1378 1378 -, aca dbSNP:748289906
1385 1385 c, t dbSNP:201030784
1387 1387 g, t dbSNP:768717142
1394 1394 c, g, t dbSNP:570378547
1399 1399 g, t dbSNP:561591789
1401 1401 a, g dbSNP:771016534
1404 1404 c, t dbSNP:749525283
1410 1410 a, g dbSNP:778042648
1412 1412 c, t dbSNP:770123245
1413 1413 a, c, g dbSNP:139171143
1418 1418 c, t dbSNP:377178938
1424 1424 c, t dbSNP:372792059
1427 1427 c, t dbSNP:145456588
1428 1428 a, c dbSNP:757902679
1430 1430 a, c dbSNP:750097789
1435 1435 a, t dbSNP:764935677
1436 1436 c, t dbSNP:151193377
1440 1440 a, g dbSNP:753697359
1442 1442 c, t dbSNP:545473686
1445 1445 a, c dbSNP:763943642
1450 1450 a, c dbSNP:760738362
1452 1452 c, t dbSNP:775550642
1453 1453 a, g, t dbSNP:528584911
1457 1457 a, g dbSNP:376713095
1461 1461 c, g dbSNP:374577354
1466 1466 c, t dbSNP:369916656
1467 1467 c, t dbSNP:781565995
1469 1469 c, t dbSNP:768977197
1472 1472 c, t dbSNP:373293781
1473 1473 a, g dbSNP:780592951
1475 1475 a, g dbSNP:757908339
1476 1476 a, c dbSNP:749950366
1477 1477 c, t dbSNP:575920043
1479 1479 c, t dbSNP:757004425
1482 1482 a, g dbSNP:753512613
1484 1484 c, t dbSNP:764033389
1485 1485 c, t dbSNP:755963482
1489 1489 c, t dbSNP:368910775
1495 1495 g, t dbSNP:767497740
1496 1496 c, g dbSNP:559273758
1497 1497 a, g, t dbSNP:765189193
1498 1498 c, t dbSNP:747566295
1499 1499 a, g dbSNP:55806270
1500 1500 a, c dbSNP:769063090
1512 1512 a, g, t dbSNP:775650317
1515 1515 c, t dbSNP:772645368
1517 1517 c, t dbSNP:371649855
1518 1518 c, g, t dbSNP:557192611
1525 1525 a, c dbSNP:756806532
1526 1526 a, c dbSNP:749007499
1530 1530 a, g dbSNP:2229199
1531 1531 g, t dbSNP:755764652
1532 1532 a, g dbSNP:752553401
1533 1533 a, g dbSNP:767170942
1536 1536 c, t dbSNP:2229200
1540 1540 g, t dbSNP:751419232
1544 1544 a, g dbSNP:765346320
1554 1554 c, t dbSNP:12940036
1558 1558 c, t dbSNP:56067981
1559 1559 a, g dbSNP:558514175
1596 1596 -, aatc dbSNP:768893993
1610 1610 a, g dbSNP:369991502
1618 1618 c, t dbSNP:538687901
1626 1626 a, g dbSNP:566574941
1635 1635 c, t dbSNP:552977083
1637 1637 c, t dbSNP:12936083
1647 1647 g, t dbSNP:781081435
1673 1673 -, g dbSNP:541685358
1699 1699 a, g dbSNP:548395785
1706 1706 a, g dbSNP:567298349
1732 1732 a, g dbSNP:529933298
1760 1760 c, t dbSNP:551251860
1762 1762 c, t dbSNP:12950092
1780 1780 a, g dbSNP:754851323
1790 1790 a, c dbSNP:531149063
1794 1794 g, t dbSNP:371539398
1805 1805 a, c dbSNP:751557446
1808 1808 g, t dbSNP:779969688
1826 1826 -, t dbSNP:773327003
1826 1826 -, t dbSNP:202016238
1827 1827 a, g dbSNP:571770417
1852 1852 c, t dbSNP:779847585
1859 1859 a, g dbSNP:551684568
1927 1927 c, t dbSNP:77966333
1930 1930 c, g dbSNP:3514
1938 1938 c, t dbSNP:750101785
1944 1944 c, t dbSNP:559433226
1949 1949 a, c dbSNP:767704520
1994 1994 c, g dbSNP:542749907
2011 2011 -, gtca dbSNP:531934737
2034 2034 c, t dbSNP:529231762
2054 2054 c, t dbSNP:751958039
2055 2055 a, g dbSNP:749958750
2065 2065 c, g dbSNP:564549054
2103 2103 c, t dbSNP:144554248
2108 2108 c, t dbSNP:761563733
2136 2136 c, t dbSNP:754125849
2139 2139 c, t dbSNP:374298019
2160 2160 a, g dbSNP:575460337
2169 2169 c, g dbSNP:558674000
2236 2236 -, tgg dbSNP:746315302
2236 2236 g, t dbSNP:1053754
2238 2238 a, g dbSNP:1053751
2239 2239 a, g dbSNP:553081443
2241 2241 a, g dbSNP:536398300
2244 2244 -, g dbSNP:536198075
2247 2247 -, ggg dbSNP:767446594
2248 2248 -, g dbSNP:201114697
2249 2249 -, gga dbSNP:368356907
2250 2250 a, g dbSNP:3087773
2250 2250 -, g, gag dbSNP:538982347
2251 2251 a, g dbSNP:368116445
2253 2253 -, aac dbSNP:367938507
2253 2253 c, g dbSNP:112548629
2272 2272 -, ac dbSNP:570601986
2281 2281 a, g dbSNP:777911516
2296 2296 c, t dbSNP:374217615
2298 2298 g, t dbSNP:113366718
2305 2305 -, tgag dbSNP:529707167
2330 2330 a, g, t dbSNP:8834
2345 2345 a, g dbSNP:761256289
2348 2348 c, t dbSNP:575836707
2351 2351 c, g dbSNP:375660349
2361 2361 a, c, g, t dbSNP:7774
2369 2369 a, g dbSNP:192238331
2395 2395 c, t dbSNP:369145199
2396 2396 a, g dbSNP:549064723
2399 2399 c, g dbSNP:9914357
2415 2415 c, g dbSNP:759610943
2423 2423 g, t dbSNP:116802199
2424 2424 g, t dbSNP:186131152

Target ORF information:

RefSeq Version NM_000080
Organism Homo sapiens (human)
Definition Homo sapiens cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000080

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.