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CHRNE cDNA ORF clone, Homo sapiens (human)

Gene Symbol CHRNE
Entrez Gene ID 1145
Full Name cholinergic receptor, nicotinic, epsilon (muscle)
Synonyms ACHRE, CMS1D, CMS1E, CMS2A, CMS4A, CMS4B, CMS4C, FCCMS, SCCMS
General protein information
Preferred Names
acetylcholine receptor subunit epsilon
Names
acetylcholine receptor subunit epsilon
AchR epsilon subunit
acetylcholine receptor, nicotinic, epsilon (muscle)
cholinergic receptor, nicotinic, epsilon polypeptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17p13.2

Summary Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

100725

Disorder Html: Myasthenic syndrome, slow-channel congenital, 601462 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_011523631 XP_011521933 acetylcholine receptor subunit epsilon isoform X1
NM_000080 NP_000071 acetylcholine receptor subunit epsilon precursor



Homo sapiens (human) CHRNE NP_000071.1
Pan troglodytes (chimpanzee) CHRNE XP_523556.4
Macaca mulatta (Rhesus monkey) CHRNE XP_001097094.1
Canis lupus familiaris (dog) CHRNE XP_536608.3
Bos taurus (cattle) CHRNE NP_776697.1
Mus musculus (house mouse) Chrne NP_033733.1
Rattus norvegicus (Norway rat) Chrne NP_058890.1
Xenopus (Silurana) tropicalis (western clawed frog) chrne XP_002941867.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHRNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu56906 XM_011523631 PREDICTED: Homo sapiens cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu20760
NM_000080 Homo sapiens cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu56906
Clone ID Related Accession (Same CDS sequence) XM_011523631
Accession Version XM_011523631.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product acetylcholine receptor subunit epsilon isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010718.17) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGGTTCCTTA TTTTCGTCAT GGTGGTCGCC ACGCTCATTG
TCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011521933.1
CDS66..911
Misc Feature(1)135..785(+)
Translation

Target ORF information:

RefSeq Version XM_011523631
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011523631

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGGTTCCTTA TTTTCGTCAT GGTGGTCGCC ACGCTCATTG
TCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20760
Clone ID Related Accession (Same CDS sequence) NM_000080
Accession Version NM_000080.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-MAY-2015
Organism Homo sapiens (human)
Product acetylcholine receptor subunit epsilon precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X66403.1 and AC233723.2. This sequence is a reference standard in the RefSeqGene project. On Apr 20, 2010 this sequence version replaced gi:38327653. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X66403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159607 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000071.1
CDS12..1493
Misc Feature(1)72..1436(+)
Misc Feature(2)81..731(+)
Misc Feature(3)729..803(+)
Misc Feature(4)750..1433(+)
Misc Feature(5)828..884(+)
Misc Feature(6)930..995(+)
Misc Feature(7)1380..1451(+)
Exon (1)1..57
Gene:CHRNE
Gene Synonym:
Exon (2)58..200
Gene:CHRNE
Gene Synonym:
Exon (3)201..245
Gene:CHRNE
Gene Synonym:
Exon (4)246..355
Gene:CHRNE
Gene Synonym:
Exon (5)356..511
Gene:CHRNE
Gene Synonym:
Exon (6)512..612
Gene:CHRNE
Gene Synonym:
Exon (7)613..813
Gene:CHRNE
Gene Synonym:
Exon (8)814..928
Gene:CHRNE
Gene Synonym:
Exon (9)929..1043
Gene:CHRNE
Gene Synonym:
Exon (10)1044..1230
Gene:CHRNE
Gene Synonym:
Exon (11)1231..1337
Gene:CHRNE
Gene Synonym:
Exon (12)1338..2460
Gene:CHRNE
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000080
Organism Homo sapiens (human)
Definition Homo sapiens cholinergic receptor, nicotinic, epsilon (muscle) (CHRNE), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000080

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCAAGGG CTCCGCTTGG GGTCCTGCTC CTCTTGGGGC TTCTCGGCAG GGGTGTGGGG 
AAGAACGAGG AACTGCGTCT TTATCACCAT CTCTTCAACA ACTATGACCC AGGAAGCCGG
CCAGTGCGGG AGCCTGAGGA TACTGTCACC ATCAGCCTCA AGGTCACCCT GACGAATCTC
ATCTCACTGA ATGAAAAAGA GGAGACTCTC ACCACTAGCG TCTGGATTGG AATCGATTGG
CAGGATTACC GACTCAACTA CAGCAAGGAC GACTTTGGGG GTATAGAAAC CCTGCGAGTC
CCTTCAGAAC TCGTGTGGCT GCCAGAGATT GTGCTGGAAA ACAATATTGA TGGCCAGTTC
GGAGTGGCCT ACGACGCCAA CGTGCTCGTC TACGAGGGCG GCTCCGTGAC GTGGCTGCCT
CCGGCCATCT ACCGCAGCGT CTGCGCAGTG GAGGTCACCT ACTTCCCCTT CGATTGGCAG
AACTGTTCGC TTATTTTCCG CTCTCAGACG TACAATGCCG AAGAGGTGGA GTTCACTTTT
GCCGTAGACA ACGACGGCAA GACCATCAAC AAGATCGACA TCGACACAGA GGCCTATACT
GAGAACGGCG AGTGGGCCAT CGACTTCTGC CCGGGGGTGA TCCGCCGCCA CCACGGTGGC
GCCACCGACG GCCCAGGGGA GACTGACGTC ATCTACTCGC TCATCATCCG CCGGAAGCCG
CTCTTCTACG TCATTAACAT CATCGTGCCC TGTGTGCTCA TCTCGGGCCT GGTGCTGCTC
GCCTACTTCC TGCCGGCGCA GGCCGGCGGC CAGAAATGCA CGGTCTCCAT CAACGTCCTG
CTCGCCCAGA CCGTCTTCTT GTTCCTCATT GCCCAGAAAA TCCCAGAGAC TTCTCTGAGC
GTGCCGCTCC TGGGCAGGTT CCTTATTTTC GTCATGGTGG TCGCCACGCT CATTGTCATG
AATTGCGTCA TCGTGCTCAA CGTGTCCCAG CGGACGCCCA CCACCCACGC CATGTCCCCG
CGGCTGCGCC ACGTTCTCCT GGAGCTGCTG CCGCGCCTCC TGGGCTCCCC GCCGCCGCCC
GAGGCCCCCC GGGCCGCCTC GCCCCCAAGG CGGGCGTCGT CGGTGGGCTT ATTGCTCCGC
GCGGAGGAGC TGATACTGAA AAAGCCACGG AGCGAGCTCG TGTTTGAGGG GCAGAGGCAC
CGGCAGGGGA CCTGGACGGC TGCCTTCTGC CAGAGCCTGG GCGCCGCCGC CCCCGAGGTC
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCCGAGAGCA CGAGAGATCA GGAGGCCACC
GGCGAGGAAG TGTCCGACTG GGTGCGCATG GGGAATGCCC TTGACAACAT CTGCTTCTGG
GCCGCTCTGG TGCTCTTCAG CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC
CGAGTGCCTG ATCTCCCCTA CGCGCCGTGT ATCCAGCCTT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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