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CHRNG cDNA ORF clone, Homo sapiens (human)

Gene Symbol CHRNG
Entrez Gene ID 1146
Full Name cholinergic receptor, nicotinic, gamma (muscle)
Synonyms ACHRG
General protein information
Preferred Names
acetylcholine receptor subunit gamma
Names
acetylcholine receptor subunit gamma
acetylcholine receptor, muscle, gamma subunit
acetylcholine receptor, nicotinic, gamma (muscle)
cholinergic receptor, nicotinic, gamma polypeptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q37.1

Summary The mammalian muscle-type acetylcholine receptor is a transmembrane pentameric glycoprotein with two alpha subunits, one beta, one delta, and one epsilon (in adult skeletal muscle) or gamma (in fetal and denervated muscle) subunit. This gene, which encodes the gamma subunit, is expressed prior to the thirty-third week of gestation in humans. The gamma subunit of the acetylcholine receptor plays a role in neuromuscular organogenesis and ligand binding and disruption of gamma subunit expression prevents the correct localization of the receptor in cell membranes. Mutations in this gene cause Escobar syndrome and a lethal form of multiple pterygium syndrome. Muscle-type acetylcholine receptor is the major antigen in the autoimmune disease myasthenia gravis.[provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

100730

Disorder Html: Myasthenia gravis, neonatal transient (2); Escobar syndrome, 265000





The following CHRNG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19621 NM_005199 Homo sapiens cholinergic receptor, nicotinic, gamma (muscle) (CHRNG), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$300.30
$429.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu19621
Accession Version NM_005199.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product acetylcholine receptor subunit gamma precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK125362.1. This sequence is a reference standard in the RefSeqGene project. On Mar 24, 2005 this sequence version replaced gi:7382453. Summary: The mammalian muscle-type acetylcholine receptor is a transmembrane pentameric glycoprotein with two alpha subunits, one beta, one delta, and one epsilon (in adult skeletal muscle) or gamma (in fetal and denervated muscle) subunit. This gene, which encodes the gamma subunit, is expressed prior to the thirty-third week of gestation in humans. The gamma subunit of the acetylcholine receptor plays a role in neuromuscular organogenesis and ligand binding and disruption of gamma subunit expression prevents the correct localization of the receptor in cell membranes. Mutations in this gene cause Escobar syndrome and a lethal form of multiple pterygium syndrome. Muscle-type acetylcholine receptor is the major antigen in the autoimmune disease myasthenia gravis.[provided by RefSeq, Sep 2009]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK125362.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2162946, SAMEA962347 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCATGGGG GCCAGGGGCC GCTGCTCCTC CTGCTGCTGC TGGCTGTCTG CCTGGGGGCC 
CAGGGCCGGA ACCAGGAGGA GCGCCTGCTC GCAGACCTGA TGCAAAACTA CGACCCCAAC
CTGCGGCCCG CGGAACGAGA CTCGGATGTG GTCAATGTCA GCCTGAAGCT AACCCTCACC
AACCTCATCT CCCTGAACGA GCGAGAGGAA GCCCTCACCA CCAATGTCTG GATAGAGATG
CAGTGGTGCG ACTATCGCCT GCGCTGGGAT CCGCGAGACT ACGAAGGCCT GTGGGTGCTG
AGGGTGCCGT CCACCATGGT GTGGCGGCCG GATATCGTGC TGGAGAACAA CGTGGACGGT
GTCTTCGAGG TGGCCCTCTA CTGCAATGTG CTCGTGTCCC CTGACGGCTG TATCTACTGG
CTGCCGCCTG CCATCTTCCG TTCCGCCTGC TCTATCTCAG TCACCTACTT CCCCTTCGAC
TGGCAGAACT GCTCCCTTAT CTTCCAGTCC CAGACTTACA GCACCAATGA GATTGATCTG
CAGCTGAGTC AGGAAGATGG CCAGACCATC GAGTGGATTT TCATTGACCC TGAGGCCTTC
ACAGAGAATG GGGAGTGGGC CATCCAGCAC CGACCAGCCA AGATGCTCCT GGACCCAGCG
GCGCCAGCCC AGGAAGCAGG CCACCAGAAG GTGGTGTTCT ACCTGCTCAT CCAGCGCAAG
CCCCTCTTCT ACGTCATCAA CATCATCGCC CCCTGTGTGC TCATCTCCTC TGTCGCCATC
CTCATCCACT TCCTTCCTGC CAAGGCTGGG GGCCAGAAGT GTACCGTCGC CATCAACGTG
CTCCTGGCCC AGACTGTCTT CCTCTTCCTT GTGGCCAAGA AGGTGCCTGA AACCTCCCAG
GCGGTGCCAC TCATCAGCAA GTACCTGACC TTCCTCCTGG TGGTGACCAT CCTCATTGTC
GTGAATGCTG TGGTTGTGCT CAATGTCTCC TTGCGGTCTC CACACACACA CTCCATGGCC
CGAGGGGTCC GCAAGGTGTT CCTGAGGCTC TTGCCCCAGC TGCTGAGGAT GCACGTTCGC
CCGCTGGCCC CGGCAGCTGT GCAGGACACC CAGTCCCGGC TACAGAATGG CTCCTCGGGA
TGGTCGATCA CAACTGGGGA GGAGGTGGCC CTCTGCCTGC CTCGCAGTGA ACTCCTCTTC
CAGCAGTGGC AGCGGCAAGG GCTGGTGGCG GCAGCGCTGG AGAAGCTAGA GAAAGGCCCG
GAGTTAGGGC TGAGCCAGTT CTGTGGCAGC CTGAAGCAGG CTGCCCCAGC CATCCAGGCC
TGTGTGGAAG CCTGCAACCT CATTGCCTGT GCCCGGCACC AGCAGAGTCA CTTTGACAAT
GGGAATGAGG AGTGGTTCCT GGTGGGCCGA GTGCTGGACC GCGTCTGCTT CCTGGCCATG
CTCTCGCTCT TCATCTGTGG CACAGCTGGC ATCTTCCTCA TGGCCCACTA CAACCGGGTG
CCGGCCCTGC CATTCCCTGG AGATCCACGC CCCTACCTGC CCTCACCAGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_005190.4
CDS22..1575
Misc Feature(1)13..15(+)
Misc Feature(2)88..1506(+)
Misc Feature(3)97..744(+)
Misc Feature(4)742..816(+)
Misc Feature(5)763..1497(+)
Misc Feature(6)844..900(+)
Misc Feature(7)946..1011(+)
Misc Feature(8)1444..1506(+)
Exon (1)1..76
Gene:CHRNG
Gene Synonym:
Exon (2)77..216
Gene:CHRNG
Gene Synonym:
Exon (3)217..261
Gene:CHRNG
Gene Synonym:
Exon (4)262..371
Gene:CHRNG
Gene Synonym:
Exon (5)372..527
Gene:CHRNG
Gene Synonym:
Exon (6)528..625
Gene:CHRNG
Gene Synonym:
Exon (7)626..826
Gene:CHRNG
Gene Synonym:
Exon (8)827..941
Gene:CHRNG
Gene Synonym:
Exon (9)942..1056
Gene:CHRNG
Gene Synonym:
Exon (10)1057..1270
Gene:CHRNG
Gene Synonym:
Exon (11)1271..1401
Gene:CHRNG
Gene Synonym:
Exon (12)1402..2187
Gene:CHRNG
Gene Synonym:
Translation
Position Chain Variation Link
9 9 c, g dbSNP:369418719
13 13 a, t dbSNP:186758675
26 26 a, t dbSNP:775592546
29 29 a, g dbSNP:761470012
34 34 c, t dbSNP:267606725
41 41 c, t dbSNP:200661257
42 42 a, g dbSNP:138991640
49 49 c, t dbSNP:774823451
51 51 c, t dbSNP:371835418
58 58 c, t dbSNP:762230262
59 59 c, t dbSNP:767799187
67 67 a, g dbSNP:750086403
78 78 c, g dbSNP:753421728
81 81 c, t dbSNP:759155853
86 86 a, g dbSNP:764775222
88 88 a, c, t dbSNP:374188367
89 89 a, g dbSNP:528366118
90 90 a, g dbSNP:777779730
93 93 c, t dbSNP:143418126
95 95 a, t dbSNP:201453316
103 103 c, t dbSNP:140623763
104 104 a, g dbSNP:377704298
105 105 c, t dbSNP:2573202
106 106 a, c dbSNP:768733912
111 111 c, t dbSNP:778912401
112 112 g, t dbSNP:748681430
119 119 c, t dbSNP:772604725
124 124 a, c dbSNP:773574226
133 133 a, g dbSNP:761163308
134 134 -, c dbSNP:752475887
140 140 a, c, g dbSNP:771198156
142 142 a, c dbSNP:759209024
145 145 c, t dbSNP:770466863
146 146 a, c, g dbSNP:148468628
150 150 c, g, t dbSNP:151276788
151 151 a, g dbSNP:186589083
152 152 c, t dbSNP:140462342
153 153 a, g dbSNP:141402683
157 157 c, t dbSNP:121912672
158 158 a, g dbSNP:755148348
164 164 c, t dbSNP:370022034
165 165 a, g dbSNP:143153750
176 176 a, g dbSNP:373024081
188 188 a, c dbSNP:376314818
190 190 c, g dbSNP:759163681
192 192 a, g dbSNP:778414287
195 195 c, t dbSNP:747410722
200 200 c, t dbSNP:771341746
204 204 a, c dbSNP:776814462
215 215 c, t dbSNP:745547832
219 219 c, t dbSNP:148982752
220 220 a, g dbSNP:544552194
223 223 a, c, t dbSNP:764266722
224 224 g, t dbSNP:757339715
233 233 a, c dbSNP:781781015
239 239 c, t dbSNP:374320160
242 242 a, c dbSNP:756560983
244 244 a, g dbSNP:780516350
245 245 a, g dbSNP:202052789
261 261 a, g dbSNP:768228497
261 261 -, g dbSNP:777809198
270 270 c, t dbSNP:376744239
271 271 a, g dbSNP:771588131
277 277 c, t dbSNP:777219451
278 278 a, g, t dbSNP:550521607
284 284 a, g dbSNP:567164720
285 285 c, t dbSNP:536328888
289 289 a, g dbSNP:201502519
291 291 c, t dbSNP:774448910
293 293 c, g, t dbSNP:762046159
294 294 a, g, t dbSNP:773463196
296 296 a, g dbSNP:767049166
300 300 c, g dbSNP:754362293
303 303 c, t dbSNP:183500569
304 304 a, g dbSNP:369269183
309 309 -, c dbSNP:758543707
323 323 a, g dbSNP:752319674
330 330 a, g dbSNP:148012978
338 338 c, t dbSNP:777374587
341 341 g, t dbSNP:267606726
346 346 c, t dbSNP:150697820
347 347 a, g dbSNP:147136675
348 348 a, g dbSNP:150809496
350 350 c, t dbSNP:745758214
351 351 a, g dbSNP:149931943
358 358 a, g dbSNP:139633799
369 369 c, t dbSNP:749001081
372 372 c, t dbSNP:767046286
378 378 c, t dbSNP:749995118
379 379 a, c, g dbSNP:755562229
387 387 c, t dbSNP:753759008
388 388 a, g dbSNP:199937736
397 397 c, t dbSNP:778805366
403 403 c, t dbSNP:747269970
414 414 c, t dbSNP:771229265
415 415 a, g dbSNP:781249093
418 418 -, t dbSNP:780249576
422 422 -, ct dbSNP:747067203
423 423 -, t dbSNP:768867302
426 426 c, t dbSNP:746019142
427 427 a, g dbSNP:769760285
433 433 a, t dbSNP:775982805
439 439 c, t dbSNP:763399820
441 441 a, c, g dbSNP:201762781
445 445 c, g dbSNP:762142413
446 446 c, t dbSNP:767254124
447 447 a, g, t dbSNP:375894671
449 449 c, g dbSNP:765746795
458 458 c, t dbSNP:753851324
460 460 c, t dbSNP:754928609
461 461 a, g dbSNP:144323121
463 463 a, t dbSNP:752593077
464 464 c, g dbSNP:758114725
465 465 c, t dbSNP:142435378
466 466 a, g dbSNP:2289080
479 479 -, a dbSNP:774279192
481 481 a, g dbSNP:756232748
488 488 a, g dbSNP:201970994
490 490 c, t dbSNP:780067480
492 492 c, t dbSNP:143272752
495 495 c, t dbSNP:145907618
498 498 c, g, t dbSNP:13391285
499 499 a, c, g dbSNP:748609408
514 514 -, t dbSNP:748505348
515 515 c, t dbSNP:766623295
516 516 c, g dbSNP:760199351
521 521 c, t dbSNP:766022014
529 529 c, t dbSNP:201865286
531 531 c, t dbSNP:766650311
538 538 -, taca dbSNP:765868304
554 554 c, t dbSNP:766193925
564 564 a, g dbSNP:753990044
570 570 a, t dbSNP:755060349
571 571 c, t dbSNP:778912321
573 573 -, g dbSNP:773842433
578 578 a, t dbSNP:753280990
591 591 c, t dbSNP:758872639
592 592 a, c, g dbSNP:189660100
594 594 a, g dbSNP:771417982
605 605 c, t dbSNP:780996760
614 614 a, c dbSNP:138653046
617 617 c, t dbSNP:769453177
629 629 a, g dbSNP:747094975
636 636 a, g dbSNP:748812705
641 641 c, t dbSNP:768145897
643 643 a, g dbSNP:200473548
645 645 c, t dbSNP:369008934
652 652 c, t dbSNP:761688091
653 653 a, c, g dbSNP:370116501
658 658 a, g dbSNP:760442965
659 659 a, c dbSNP:765437470
665 665 c, t dbSNP:775776788
671 671 c, t dbSNP:762950375
676 676 c, g dbSNP:764180899
680 680 c, t dbSNP:752137444
681 681 a, g dbSNP:200224934
682 682 g, t dbSNP:148066463
683 683 c, t dbSNP:750873458
684 684 a, g dbSNP:150556220
686 686 c, t dbSNP:779573860
688 688 a, g dbSNP:748912147
692 692 a, g dbSNP:754467232
693 693 a, g dbSNP:544444996
717 717 a, g dbSNP:778445156
724 724 c, g dbSNP:747624528
731 731 a, t dbSNP:772048048
732 732 a, c, t dbSNP:773121173
735 735 g, t dbSNP:17838626
736 736 c, t dbSNP:121912670
737 737 a, g dbSNP:765407839
738 738 c, g dbSNP:764114084
743 743 a, c dbSNP:774616155
751 751 -, a dbSNP:778730695
753 753 c, t dbSNP:761969308
754 754 a, g dbSNP:557970419
755 755 -, tca dbSNP:747062636
756 756 -, catcaa dbSNP:755046404
756 756 c, t dbSNP:750890018
758 758 a, t dbSNP:139998228
768 768 c, t dbSNP:766716676
773 773 c, t dbSNP:374951822
774 774 -, ct dbSNP:767503038
775 775 c, t dbSNP:145847096
785 785 a, t dbSNP:778517594
788 788 c, t dbSNP:747716114
795 795 c, t dbSNP:777027483
796 796 a, g dbSNP:138125827
810 810 c, t dbSNP:777692827
819 819 g, t dbSNP:746910885
827 827 -, t dbSNP:778176530
829 829 c, g dbSNP:771527913
830 830 a, g dbSNP:777001051
831 831 c, g dbSNP:759950871
833 833 a, g dbSNP:765528492
834 834 a, c, t dbSNP:200595788
840 840 a, g dbSNP:763707620
842 842 a, g dbSNP:751132652
846 846 c, t dbSNP:199593123
847 847 a, g dbSNP:554942571
850 850 a, g dbSNP:750265817
858 858 c, t dbSNP:200660641
859 859 a, g dbSNP:779955475
862 862 c, t dbSNP:749060431
899 899 a, g dbSNP:772470930
903 903 g, t dbSNP:777842737
906 906 a, g dbSNP:747181326
910 910 c, g dbSNP:201857172
923 923 c, t dbSNP:777208622
924 924 a, g dbSNP:200738933
928 928 c, t dbSNP:770179580
934 934 a, g dbSNP:142582718
936 936 c, t dbSNP:763283891
937 937 a, t dbSNP:763797300
949 949 a, g, t dbSNP:145830034
958 958 c, t dbSNP:753762944
962 962 a, t dbSNP:778440648
972 972 a, c dbSNP:75369104
977 977 c, t dbSNP:764892395
981 981 c, g, t dbSNP:149535359
982 982 a, g dbSNP:138637669
983 983 c, t dbSNP:746007534
988 988 a, g dbSNP:756184613
990 990 c, t dbSNP:767099064
999 999 c, g dbSNP:749744672
1000 1000 c, g dbSNP:769092268
1002 1002 c, g dbSNP:779181232
1004 1004 -, tgc dbSNP:771407292
1004 1004 a, g dbSNP:372687247
1011 1011 c, t dbSNP:771680359
1012 1012 c, t dbSNP:772992979
1015 1015 c, t dbSNP:567899708
1016 1016 a, g dbSNP:375987895
1017 1017 a, g dbSNP:113978875
1033 1033 c, t dbSNP:776733115
1036 1036 a, g dbSNP:759353019
1040 1040 c, g dbSNP:772316123
1042 1042 c, t dbSNP:752487562
1043 1043 a, c, g dbSNP:141485470
1045 1045 c, g dbSNP:750582540
1050 1050 c, g dbSNP:756205168
1051 1051 c, t dbSNP:554907332
1052 1052 a, g dbSNP:753880381
1057 1057 g, t dbSNP:775309134
1067 1067 -, g dbSNP:34630130
1074 1074 a, g dbSNP:538950277
1077 1077 c, t dbSNP:768545950
1080 1080 a, g dbSNP:200220463
1089 1089 a, g dbSNP:774177641
1095 1095 c, g, t dbSNP:761581720
1096 1096 a, g dbSNP:16829208
1097 1097 c, t dbSNP:759547367
1099 1099 a, c dbSNP:200263643
1100 1100 a, g dbSNP:140875617
1103 1103 c, t dbSNP:759015914
1112 1112 c, t dbSNP:200208278
1113 1113 a, g dbSNP:144846001
1115 1115 c, g dbSNP:757694823
1116 1116 a, g dbSNP:781000671
1117 1117 a, g, t dbSNP:143522081
1120 1120 c, g dbSNP:150928642
1121 1121 c, t dbSNP:74691009
1136 1136 c, t dbSNP:145433186
1138 1138 c, t dbSNP:774265808
1139 1139 a, g dbSNP:144755776
1144 1144 c, t dbSNP:771796518
1148 1148 a, g dbSNP:776739154
1149 1149 c, t dbSNP:143840391
1152 1152 c, t dbSNP:765348696
1157 1157 c, t dbSNP:775689070
1158 1158 a, g dbSNP:147318482
1166 1166 c, t dbSNP:774121021
1167 1167 a, g dbSNP:200065628
1168 1168 a, g dbSNP:757713399
1169 1169 c, t dbSNP:144961313
1174 1174 a, g dbSNP:750718050
1175 1175 c, t dbSNP:755837139
1176 1176 c, t dbSNP:370313191
1180 1180 c, g dbSNP:753412578
1187 1187 c, t dbSNP:754416840
1195 1195 c, t dbSNP:778834766
1196 1196 a, g dbSNP:748029278
1201 1201 c, g dbSNP:771886490
1204 1204 c, t dbSNP:777663481
1205 1205 a, g dbSNP:375087506
1214 1214 a, t dbSNP:770113355
1218 1218 c, g dbSNP:149893884
1223 1223 a, g dbSNP:763132076
1228 1228 c, t dbSNP:768802998
1234 1234 c, t dbSNP:760473901
1237 1237 c, t dbSNP:762445911
1241 1241 a, g dbSNP:767884780
1242 1242 c, g dbSNP:750854483
1250 1250 c, g, t dbSNP:760940301
1251 1251 a, c, g dbSNP:144948513
1254 1254 a, t dbSNP:368057603
1256 1256 c, t dbSNP:778354120
1262 1262 a, g dbSNP:752173448
1263 1263 a, g dbSNP:758411973
1264 1264 a, t dbSNP:777560454
1280 1280 c, t dbSNP:146674765
1281 1281 a, g dbSNP:376569365
1287 1287 a, g dbSNP:773789491
1288 1288 c, g dbSNP:747433309
1294 1294 a, g dbSNP:771437689
1307 1307 a, g dbSNP:145607890
1314 1314 c, g dbSNP:759881185
1322 1322 c, t dbSNP:764938199
1326 1326 c, t dbSNP:368612307
1330 1330 a, g dbSNP:762540344
1333 1333 a, g dbSNP:372355341
1340 1340 c, t dbSNP:763468045
1354 1354 c, t dbSNP:751549903
1357 1357 -, a dbSNP:753632988
1358 1358 a, g dbSNP:560337012
1361 1361 c, t dbSNP:372237914
1363 1363 a, c dbSNP:767544170
1364 1364 a, c, t dbSNP:139108448
1367 1367 c, t dbSNP:779289856
1375 1375 c, t dbSNP:748268862
1376 1376 a, g dbSNP:758083179
1377 1377 a, g dbSNP:758536494
1394 1394 g, t dbSNP:140836265
1396 1396 a, g dbSNP:747089392
1398 1398 a, c dbSNP:771486058
1399 1399 a, g dbSNP:138232636
1401 1401 c, t dbSNP:746237092
1402 1402 a, g dbSNP:143800157
1404 1404 g, t dbSNP:773983788
1408 1408 -, gag dbSNP:761724047
1413 1413 a, g dbSNP:761419988
1420 1420 a, c dbSNP:771657153
1429 1429 c, t dbSNP:121912671
1430 1430 a, g dbSNP:143306273
1438 1438 g, t dbSNP:760734396
1441 1441 c, t dbSNP:199767964
1442 1442 a, g dbSNP:16829216
1443 1443 c, t dbSNP:2099489
1444 1444 a, g dbSNP:764228635
1449 1449 c, t dbSNP:751748690
1458 1458 c, t dbSNP:757352527
1459 1459 a, g dbSNP:781211383
1466 1466 c, t dbSNP:750474176
1467 1467 a, g dbSNP:375174291
1478 1478 a, g dbSNP:751750221
1486 1486 a, g dbSNP:531106516
1487 1487 c, g dbSNP:749615540
1498 1498 c, t dbSNP:769004359
1504 1504 a, g dbSNP:778450593
1505 1505 c, g dbSNP:747761957
1513 1513 a, t dbSNP:771818429
1516 1516 c, t dbSNP:369539666
1517 1517 a, g dbSNP:373096766
1523 1523 c, t dbSNP:185590413
1524 1524 a, g dbSNP:776565802
1527 1527 c, t dbSNP:759265096
1537 1537 c, t dbSNP:71421651
1540 1540 a, g dbSNP:574529560
1549 1549 c, t dbSNP:762066089
1550 1550 a, g dbSNP:767708955
1551 1551 c, t dbSNP:750564080
1562 1562 c, g dbSNP:373560478
1567 1567 a, c, g dbSNP:756116890
1572 1572 c, t dbSNP:754319350
1577 1577 a, c dbSNP:755328330
1582 1582 -, caa dbSNP:765181661
1598 1598 -, t dbSNP:755064084
1601 1601 a, g dbSNP:540310491
1605 1605 -, g dbSNP:781185631
1606 1606 -, ggaaa dbSNP:752911626
1607 1607 a, c dbSNP:748500540
1612 1612 a, c dbSNP:758181611
1613 1613 -, c dbSNP:777981583
1614 1614 a, g, t dbSNP:777563686
1616 1616 -, g dbSNP:749601749
1621 1621 a, c dbSNP:770417849
1625 1625 a, t dbSNP:377400953
1652 1652 c, t dbSNP:11690038
1701 1701 c, t dbSNP:369614677
1706 1706 c, t dbSNP:566877095
1748 1748 c, g dbSNP:577360709
1750 1750 a, g dbSNP:190449191
1775 1775 a, t dbSNP:373201998
1776 1776 c, g dbSNP:144523413
1782 1782 c, t dbSNP:147814126
1801 1801 a, g dbSNP:141353873
1816 1816 c, t dbSNP:111733445
1856 1856 c, t dbSNP:560846230
1916 1916 c, t dbSNP:529762226
1926 1926 a, g dbSNP:111802317
1957 1957 c, g dbSNP:182946513
1999 1999 g, t dbSNP:756514947
2001 2001 c, t dbSNP:532237748
2014 2014 a, g dbSNP:552059021
2019 2019 a, t dbSNP:558982566
2052 2052 a, c, t dbSNP:59295139
2054 2054 c, t dbSNP:746596667
2055 2055 a, g dbSNP:537733112
2066 2066 c, g dbSNP:548109767
2071 2071 a, g dbSNP:72991937
2085 2085 c, t dbSNP:2853459
2089 2089 a, g dbSNP:759342913
2092 2092 a, g dbSNP:554763080
2094 2094 c, t dbSNP:533973618
2100 2100 a, g dbSNP:376260548
2101 2101 g, t dbSNP:187625908
2143 2143 c, t dbSNP:72991939
2145 2145 -, t dbSNP:34664424
2150 2150 c, g dbSNP:12613412
2166 2166 -, tcttttttttt dbSNP:763100229
2166 2166 -, tcttttt dbSNP:144816964
2167 2167 -, ctttttt dbSNP:370424607
2167 2167 -, t, tt dbSNP:71398725
2167 2167 c, t dbSNP:72991940

Target ORF information:

RefSeq Version NM_005199
Organism Homo sapiens (human)
Definition Homo sapiens cholinergic receptor, nicotinic, gamma (muscle) (CHRNG), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005199

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCATGGGG GCCAGGGGCC GCTGCTCCTC CTGCTGCTGC TGGCTGTCTG CCTGGGGGCC 
CAGGGCCGGA ACCAGGAGGA GCGCCTGCTC GCAGACCTGA TGCAAAACTA CGACCCCAAC
CTGCGGCCCG CGGAACGAGA CTCGGATGTG GTCAATGTCA GCCTGAAGCT AACCCTCACC
AACCTCATCT CCCTGAACGA GCGAGAGGAA GCCCTCACCA CCAATGTCTG GATAGAGATG
CAGTGGTGCG ACTATCGCCT GCGCTGGGAT CCGCGAGACT ACGAAGGCCT GTGGGTGCTG
AGGGTGCCGT CCACCATGGT GTGGCGGCCG GATATCGTGC TGGAGAACAA CGTGGACGGT
GTCTTCGAGG TGGCCCTCTA CTGCAATGTG CTCGTGTCCC CTGACGGCTG TATCTACTGG
CTGCCGCCTG CCATCTTCCG TTCCGCCTGC TCTATCTCAG TCACCTACTT CCCCTTCGAC
TGGCAGAACT GCTCCCTTAT CTTCCAGTCC CAGACTTACA GCACCAATGA GATTGATCTG
CAGCTGAGTC AGGAAGATGG CCAGACCATC GAGTGGATTT TCATTGACCC TGAGGCCTTC
ACAGAGAATG GGGAGTGGGC CATCCAGCAC CGACCAGCCA AGATGCTCCT GGACCCAGCG
GCGCCAGCCC AGGAAGCAGG CCACCAGAAG GTGGTGTTCT ACCTGCTCAT CCAGCGCAAG
CCCCTCTTCT ACGTCATCAA CATCATCGCC CCCTGTGTGC TCATCTCCTC TGTCGCCATC
CTCATCCACT TCCTTCCTGC CAAGGCTGGG GGCCAGAAGT GTACCGTCGC CATCAACGTG
CTCCTGGCCC AGACTGTCTT CCTCTTCCTT GTGGCCAAGA AGGTGCCTGA AACCTCCCAG
GCGGTGCCAC TCATCAGCAA GTACCTGACC TTCCTCCTGG TGGTGACCAT CCTCATTGTC
GTGAATGCTG TGGTTGTGCT CAATGTCTCC TTGCGGTCTC CACACACACA CTCCATGGCC
CGAGGGGTCC GCAAGGTGTT CCTGAGGCTC TTGCCCCAGC TGCTGAGGAT GCACGTTCGC
CCGCTGGCCC CGGCAGCTGT GCAGGACACC CAGTCCCGGC TACAGAATGG CTCCTCGGGA
TGGTCGATCA CAACTGGGGA GGAGGTGGCC CTCTGCCTGC CTCGCAGTGA ACTCCTCTTC
CAGCAGTGGC AGCGGCAAGG GCTGGTGGCG GCAGCGCTGG AGAAGCTAGA GAAAGGCCCG
GAGTTAGGGC TGAGCCAGTT CTGTGGCAGC CTGAAGCAGG CTGCCCCAGC CATCCAGGCC
TGTGTGGAAG CCTGCAACCT CATTGCCTGT GCCCGGCACC AGCAGAGTCA CTTTGACAAT
GGGAATGAGG AGTGGTTCCT GGTGGGCCGA GTGCTGGACC GCGTCTGCTT CCTGGCCATG
CTCTCGCTCT TCATCTGTGG CACAGCTGGC ATCTTCCTCA TGGCCCACTA CAACCGGGTG
CCGGCCCTGC CATTCCCTGG AGATCCACGC CCCTACCTGC CCTCACCAGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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