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CHRNG cDNA ORF clone, Homo sapiens (human)

Gene Symbol CHRNG
Entrez Gene ID 1146
Full Name cholinergic receptor, nicotinic, gamma (muscle)
Synonyms ACHRG
General protein information
Preferred Names
acetylcholine receptor subunit gamma
Names
acetylcholine receptor subunit gamma
acetylcholine receptor, muscle, gamma subunit
acetylcholine receptor, nicotinic, gamma (muscle)
cholinergic receptor, nicotinic, gamma polypeptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q37.1

Summary The mammalian muscle-type acetylcholine receptor is a transmembrane pentameric glycoprotein with two alpha subunits, one beta, one delta, and one epsilon (in adult skeletal muscle) or gamma (in fetal and denervated muscle) subunit. This gene, which encodes the gamma subunit, is expressed prior to the thirty-third week of gestation in humans. The gamma subunit of the acetylcholine receptor plays a role in neuromuscular organogenesis and ligand binding and disruption of gamma subunit expression prevents the correct localization of the receptor in cell membranes. Mutations in this gene cause Escobar syndrome and a lethal form of multiple pterygium syndrome. Muscle-type acetylcholine receptor is the major antigen in the autoimmune disease myasthenia gravis.[provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

100730

Disorder Html: Myasthenia gravis, neonatal transient (2); Escobar syndrome, 265000

mRNA and Protein(s)

mRNA Protein Name
NM_005199 NP_005190 acetylcholine receptor subunit gamma precursor



Homo sapiens (human) CHRNG NP_005190.4
Pan troglodytes (chimpanzee) CHRNG XP_526063.1
Macaca mulatta (Rhesus monkey) CHRNG XP_001114133.2
Canis lupus familiaris (dog) CHRNG XP_543289.2
Bos taurus (cattle) CHRNG NP_776698.1
Mus musculus (house mouse) Chrng NP_033734.3
Rattus norvegicus (Norway rat) Chrng NP_062018.1
Gallus gallus (chicken) CHRNG NP_001026739.1
Danio rerio (zebrafish) LOC100536659 NP_001241879.1
Xenopus (Silurana) tropicalis (western clawed frog) chrng NP_001096503.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHRNG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19621
NM_005199 Homo sapiens cholinergic receptor, nicotinic, gamma (muscle) (CHRNG), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$214.50-$300.30
$429.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19621
Clone ID Related Accession (Same CDS sequence) NM_005199
Accession Version NM_005199.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1554bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product acetylcholine receptor subunit gamma precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK125362.1. This sequence is a reference standard in the RefSeqGene project. On Mar 24, 2005 this sequence version replaced gi:7382453. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK125362.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2162946, SAMEA962347 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCATGGGG GCCAGGGGCC GCTGCTCCTC CTGCTGCTGC TGGCTGTCTG CCTGGGGGCC 
CAGGGCCGGA ACCAGGAGGA GCGCCTGCTC GCAGACCTGA TGCAAAACTA CGACCCCAAC
CTGCGGCCCG CGGAACGAGA CTCGGATGTG GTCAATGTCA GCCTGAAGCT AACCCTCACC
AACCTCATCT CCCTGAACGA GCGAGAGGAA GCCCTCACCA CCAATGTCTG GATAGAGATG
CAGTGGTGCG ACTATCGCCT GCGCTGGGAT CCGCGAGACT ACGAAGGCCT GTGGGTGCTG
AGGGTGCCGT CCACCATGGT GTGGCGGCCG GATATCGTGC TGGAGAACAA CGTGGACGGT
GTCTTCGAGG TGGCCCTCTA CTGCAATGTG CTCGTGTCCC CTGACGGCTG TATCTACTGG
CTGCCGCCTG CCATCTTCCG TTCCGCCTGC TCTATCTCAG TCACCTACTT CCCCTTCGAC
TGGCAGAACT GCTCCCTTAT CTTCCAGTCC CAGACTTACA GCACCAATGA GATTGATCTG
CAGCTGAGTC AGGAAGATGG CCAGACCATC GAGTGGATTT TCATTGACCC TGAGGCCTTC
ACAGAGAATG GGGAGTGGGC CATCCAGCAC CGACCAGCCA AGATGCTCCT GGACCCAGCG
GCGCCAGCCC AGGAAGCAGG CCACCAGAAG GTGGTGTTCT ACCTGCTCAT CCAGCGCAAG
CCCCTCTTCT ACGTCATCAA CATCATCGCC CCCTGTGTGC TCATCTCCTC TGTCGCCATC
CTCATCCACT TCCTTCCTGC CAAGGCTGGG GGCCAGAAGT GTACCGTCGC CATCAACGTG
CTCCTGGCCC AGACTGTCTT CCTCTTCCTT GTGGCCAAGA AGGTGCCTGA AACCTCCCAG
GCGGTGCCAC TCATCAGCAA GTACCTGACC TTCCTCCTGG TGGTGACCAT CCTCATTGTC
GTGAATGCTG TGGTTGTGCT CAATGTCTCC TTGCGGTCTC CACACACACA CTCCATGGCC
CGAGGGGTCC GCAAGGTGTT CCTGAGGCTC TTGCCCCAGC TGCTGAGGAT GCACGTTCGC
CCGCTGGCCC CGGCAGCTGT GCAGGACACC CAGTCCCGGC TACAGAATGG CTCCTCGGGA
TGGTCGATCA CAACTGGGGA GGAGGTGGCC CTCTGCCTGC CTCGCAGTGA ACTCCTCTTC
CAGCAGTGGC AGCGGCAAGG GCTGGTGGCG GCAGCGCTGG AGAAGCTAGA GAAAGGCCCG
GAGTTAGGGC TGAGCCAGTT CTGTGGCAGC CTGAAGCAGG CTGCCCCAGC CATCCAGGCC
TGTGTGGAAG CCTGCAACCT CATTGCCTGT GCCCGGCACC AGCAGAGTCA CTTTGACAAT
GGGAATGAGG AGTGGTTCCT GGTGGGCCGA GTGCTGGACC GCGTCTGCTT CCTGGCCATG
CTCTCGCTCT TCATCTGTGG CACAGCTGGC ATCTTCCTCA TGGCCCACTA CAACCGGGTG
CCGGCCCTGC CATTCCCTGG AGATCCACGC CCCTACCTGC CCTCACCAGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005190.4
CDS22..1575
Misc Feature(1)13..15(+)
Misc Feature(2)88..1506(+)
Misc Feature(3)97..744(+)
Misc Feature(4)742..816(+)
Misc Feature(5)763..1497(+)
Misc Feature(6)844..900(+)
Misc Feature(7)946..1011(+)
Misc Feature(8)1444..1506(+)
Exon (1)1..76
Gene:CHRNG
Gene Synonym:
Exon (2)77..216
Gene:CHRNG
Gene Synonym:
Exon (3)217..261
Gene:CHRNG
Gene Synonym:
Exon (4)262..371
Gene:CHRNG
Gene Synonym:
Exon (5)372..527
Gene:CHRNG
Gene Synonym:
Exon (6)528..625
Gene:CHRNG
Gene Synonym:
Exon (7)626..826
Gene:CHRNG
Gene Synonym:
Exon (8)827..941
Gene:CHRNG
Gene Synonym:
Exon (9)942..1056
Gene:CHRNG
Gene Synonym:
Exon (10)1057..1270
Gene:CHRNG
Gene Synonym:
Exon (11)1271..1401
Gene:CHRNG
Gene Synonym:
Exon (12)1402..2187
Gene:CHRNG
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005199
Organism Homo sapiens (human)
Definition Homo sapiens cholinergic receptor, nicotinic, gamma (muscle) (CHRNG), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005199

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCATGGGG GCCAGGGGCC GCTGCTCCTC CTGCTGCTGC TGGCTGTCTG CCTGGGGGCC 
CAGGGCCGGA ACCAGGAGGA GCGCCTGCTC GCAGACCTGA TGCAAAACTA CGACCCCAAC
CTGCGGCCCG CGGAACGAGA CTCGGATGTG GTCAATGTCA GCCTGAAGCT AACCCTCACC
AACCTCATCT CCCTGAACGA GCGAGAGGAA GCCCTCACCA CCAATGTCTG GATAGAGATG
CAGTGGTGCG ACTATCGCCT GCGCTGGGAT CCGCGAGACT ACGAAGGCCT GTGGGTGCTG
AGGGTGCCGT CCACCATGGT GTGGCGGCCG GATATCGTGC TGGAGAACAA CGTGGACGGT
GTCTTCGAGG TGGCCCTCTA CTGCAATGTG CTCGTGTCCC CTGACGGCTG TATCTACTGG
CTGCCGCCTG CCATCTTCCG TTCCGCCTGC TCTATCTCAG TCACCTACTT CCCCTTCGAC
TGGCAGAACT GCTCCCTTAT CTTCCAGTCC CAGACTTACA GCACCAATGA GATTGATCTG
CAGCTGAGTC AGGAAGATGG CCAGACCATC GAGTGGATTT TCATTGACCC TGAGGCCTTC
ACAGAGAATG GGGAGTGGGC CATCCAGCAC CGACCAGCCA AGATGCTCCT GGACCCAGCG
GCGCCAGCCC AGGAAGCAGG CCACCAGAAG GTGGTGTTCT ACCTGCTCAT CCAGCGCAAG
CCCCTCTTCT ACGTCATCAA CATCATCGCC CCCTGTGTGC TCATCTCCTC TGTCGCCATC
CTCATCCACT TCCTTCCTGC CAAGGCTGGG GGCCAGAAGT GTACCGTCGC CATCAACGTG
CTCCTGGCCC AGACTGTCTT CCTCTTCCTT GTGGCCAAGA AGGTGCCTGA AACCTCCCAG
GCGGTGCCAC TCATCAGCAA GTACCTGACC TTCCTCCTGG TGGTGACCAT CCTCATTGTC
GTGAATGCTG TGGTTGTGCT CAATGTCTCC TTGCGGTCTC CACACACACA CTCCATGGCC
CGAGGGGTCC GCAAGGTGTT CCTGAGGCTC TTGCCCCAGC TGCTGAGGAT GCACGTTCGC
CCGCTGGCCC CGGCAGCTGT GCAGGACACC CAGTCCCGGC TACAGAATGG CTCCTCGGGA
TGGTCGATCA CAACTGGGGA GGAGGTGGCC CTCTGCCTGC CTCGCAGTGA ACTCCTCTTC
CAGCAGTGGC AGCGGCAAGG GCTGGTGGCG GCAGCGCTGG AGAAGCTAGA GAAAGGCCCG
GAGTTAGGGC TGAGCCAGTT CTGTGGCAGC CTGAAGCAGG CTGCCCCAGC CATCCAGGCC
TGTGTGGAAG CCTGCAACCT CATTGCCTGT GCCCGGCACC AGCAGAGTCA CTTTGACAAT
GGGAATGAGG AGTGGTTCCT GGTGGGCCGA GTGCTGGACC GCGTCTGCTT CCTGGCCATG
CTCTCGCTCT TCATCTGTGG CACAGCTGGC ATCTTCCTCA TGGCCCACTA CAACCGGGTG
CCGGCCCTGC CATTCCCTGG AGATCCACGC CCCTACCTGC CCTCACCAGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Clinical phenotype and the lack of mutations in the CHRNG, CHRND, and CHRNA1 genes in two Indian families with Escobar syndrome
Clin. Dysmorphol. 22 (2), 54-58 (2013)
Kodaganur SG, Tontanahal SJ, Sarda A, Shah MH, Bhat V and Kumar A.


book

Genome-wide meta-analyses of multiancestry cohorts identify multiple new susceptibility loci for refractive error and myopia
Nat. Genet. 45 (3), 314-318 (2013)
Verhoeven VJ, Hysi PG, Wojciechowski R, Fan Q, Guggenheim JA, Hohn R, MacGregor S, Hewitt AW, Nag A, Cheng CY, Yonova-Doing E, Zhou X, Ikram MK, Buitendijk GH, McMahon G, Kemp JP, Pourcain BS, Simpson CL, Makela KM, Lehtimaki T, Kahonen M, Paterson AD, Hosseini SM, Wong HS, Xu L, Jonas JB, Parssinen O, Wedenoja J, Yip SP, Ho DW, Pang CP, Chen LJ, Burdon KP, Craig JE, Klein BE, Klein R, Haller T, Metspalu A, Khor CC, Tai ES, Aung T, Vithana E, Tay WT, Barathi VA, Chen P, Li R, Liao J, Zheng Y, Ong RT, Doring A, Evans DM, Timpson NJ, Verkerk AJ, Meitinger T, Raitakari O, Hawthorne F, Spector TD, Karssen LC, Pirastu M, Murgia F, Ang W, Mishra A, Montgomery GW, Pennell CE, Cumberland PM, Cotlarciuc I, Mitchell P, Wang JJ, Schache M, Janmahasatian S, Igo RP Jr, Lass JH, Chew E, Iyengar SK, Gorgels TG, Rudan I, Hayward C, Wright AF, Polasek O, Vatavuk Z, Wilson JF, Fleck B, Zeller T, Mirshahi A, Muller C, Uitterlinden AG, Rivadeneira F, Vingerling JR, Hofman A, Oostra BA, Amin N, Bergen AA, Teo YY, Rahi JS, Vitart V, Williams C, Baird PN, Wong TY, Oexle K, Pfeiffer N, Mackey DA, Young TL, van Duijn CM, Saw SM, Bailey-Wilson JE, Stambolian D, Klaver CC and Hammond CJ.


book

CHRNG genotype-phenotype correlations in the multiple pterygium syndromes
J. Med. Genet. 49 (1), 21-26 (2012)
Vogt J, Morgan NV, Rehal P, Faivre L, Brueton LA, Becker K, Fryns JP, Holder S, Islam L, Kivuva E, Lynch SA, Touraine R, Wilson LC, MacDonald F and Maher ER.


book

Multiple cholinergic nicotinic receptor genes affect nicotine dependence risk in African and European Americans
Genes Brain Behav. 9 (7), 741-750 (2010)
Saccone NL, Schwantes-An TH, Wang JC, Grucza RA, Breslau N, Hatsukami D, Johnson EO, Rice JP, Goate AM and Bierut LJ.


book

Multiple distinct risk loci for nicotine dependence identified by dense coverage of the complete family of nicotinic receptor subunit (CHRN) genes
Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (4), 453-466 (2009)
Saccone NL, Saccone SF, Hinrichs AL, Stitzel JA, Duan W, Pergadia ML, Agrawal A, Breslau N, Grucza RA, Hatsukami D, Johnson EO, Madden PA, Swan GE, Wang JC, Goate AM, Rice JP and Bierut LJ.


book

Intersubunit contacts governing assembly of the mammalian nicotinic acetylcholine receptor
Neuron 14 (3), 635-644 (1995)
Kreienkamp HJ, Maeda RK, Sine SM and Taylor P.


book

Primary structure of the human muscle acetylcholine receptor. cDNA cloning of the gamma and epsilon subunits
Eur. J. Biochem. 215 (2), 229-238 (1993)
Beeson D, Brydson M, Betty M, Jeremiah S, Povey S, Vincent A and Newsom-Davis J.


book

Mapping of Col3a1 and Col6a3 to proximal murine chromosome 1 identifies conserved linkage of structural protein genes between murine chromosome 1 and human chromosome 2q
Genomics 8 (3), 477-486 (1990)
Schurr E, Skamene E, Morgan K, Chu ML and Gros P.


book

Localization of the acetylcholine receptor gamma subunit gene to human chromosome 2q32----qter
Cytogenet. Cell Genet. 52 (3-4), 124-127 (1989)
Cohen-Haguenauer O, Barton PJ, Buonanno A, Cong NV, Masset M, de Tand MF, Merlie J and Frezal J.


book

Cloning and sequence analysis of human genomic DNA encoding gamma subunit precursor of muscle acetylcholine receptor
Eur. J. Biochem. 146 (1), 15-22 (1985)
Shibahara,S., Kubo,T., Perski,H.J., Takahashi,H., Noda,M. and Numa,S.


 
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