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SLC25A25 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 [Homo sapiens (human)]

Gene Symbol SLC25A25
Entrez Gene ID 114789
Full Name solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25
Synonyms MCSC, PCSCL, SCAMC-2
General protein information
Preferred Names
calcium-binding mitochondrial carrier protein SCaMC-2
Names
calcium-binding mitochondrial carrier protein SCaMC-2
mitochondrial ATP-Mg/Pi carrier protein 3
short calcium-binding mitochondrial carrier 2
small calcium-binding mitochondrial carrier 2
solute carrier family 25, member 25, isoform a
mitochondrial Ca(2+)-dependent solute carrier protein 3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

9

9q34.11

Summary The protein encoded by this gene belongs to the family of calcium-binding mitochondrial carriers, with a characteristic mitochondrial carrier domain at the C-terminus. These proteins are found in the inner membranes of mitochondria, and function as transport proteins. They shuttle metabolites, nucleotides and cofactors through the mitochondrial membrane and thereby connect and/or regulate cytoplasm and matrix functions. This protein may function as an ATP-Mg/Pi carrier that mediates the transport of Mg-ATP in exchange for phosphate, and likely responsible for the net uptake or efflux of adenine nucleotides into or from the mitochondria. Alternatively spliced transcript variants encoding different isoforms with a common C-terminus but variable N-termini have been described for this gene. [provided by RefSeq, Jul 2012]. lac of sum
Disorder MIM:

608745

Disorder Html:





The following SLC25A25 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC25A25 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu42963 XM_005251688 PREDICTED: Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu42964 XM_006716948 PREDICTED: Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu42965 XM_006716949 PREDICTED: Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu42966 XM_005251689 PREDICTED: Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OHu12516 NM_001006642 Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $409
OHu12847 NM_001265614 Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439
OHu10171 NM_052901 Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$223.3
$319
OHu20148 NM_001006641 Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $419

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu42963
Accession Version XM_005251688.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1548bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product calcium-binding mitochondrial carrier protein SCaMC-2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008470.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGTGAGCA GTGTGTTGTG CCGCTGTGTG GCCTCCCCGC CGCCGGACGC CGCCGCCACC 
GCCGCCTCTT CGTCTGCCTC ATCGCCGGCG TCCGTGGGGG ACCCCTGCGG CGGCGCTATC
TGCGGGGGCC CGGACCACCG GCTGCGCCTG TGGAGACTCT TTCAGACGCT CGACGTCAAC
CGGGACGGCG GCCTGTGTGT CAACGACCTG GCGGTGGGGC TGCGGCGCCT GGGACTGCAC
CGCACCGAGG GCGAGCTCCA GAAAATTGTA CAAGCTGGAG ATAAGGACCT TGATGGGCAG
CTAGACTTTG AAGAATTTGT CCATTATCTC CAAGATCATG AGAAGAAGCT GAGGCTGGTG
TTTAAGAGTT TGGACAAAAA GAATGATGGA CGCATTGACG CGCAGGAGAT CATGCAGTCC
CTGCGGGACT TGGGAGTCAA GATATCTGAA CAGCAGGCAG AAAAAATTCT CAAGAGAATA
CGAACGGGCC ATTTCTGGGG CCCTGTCACC TACATGGATA AAAACGGCAC GATGACCATT
GACTGGAACG AGTGGAGAGA CTACCACCTC CTCCACCCCG TGGAAAACAT CCCCGAGATC
ATCCTCTACT GGAAGCATTC CACGATCTTT GATGTGGGTG AGAATCTAAC GGTCCCGGAT
GAGTTCACAG TGGAGGAGAG GCAGACGGGG ATGTGGTGGA GACACCTGGT GGCAGGAGGT
GGGGCAGGGG CCGTATCCAG AACCTGCACG GCCCCCCTGG ACAGGCTCAA GGTGCTCATG
CAGGTCCATG CCTCCCGCAG CAACAACATG GGCATCGTTG GTGGCTTCAC TCAGATGATT
CGAGAAGGAG GGGCCAGGTC ACTCTGGCGG GGCAATGGCA TCAACGTCCT CAAAATTGCC
CCCGAATCAG CCATCAAATT CATGGCCTAT GAGCAGATCA AGCGCCTTGT TGGTAGTGAC
CAGGAGACTC TGAGGATTCA CGAGAGGCTT GTGGCAGGGT CCTTGGCAGG GGCCATCGCC
CAGAGCAGCA TCTACCCAAT GGAGGTCCTG AAGACCCGGA TGGCGCTGCG GAAGACAGGC
CAGTACTCAG GAATGCTGGA CTGCGCCAGG AGGATCCTGG CCAGAGAGGG GGTGGCCGCC
TTCTACAAAG GCTATGTCCC CAACATGCTG GGCATCATCC CCTATGCCGG CATCGACCTT
GCAGTCTACG AGACGCTCAA GAATGCCTGG CTGCAGCACT ATGCAGTGAA CAGCGCGGAC
CCCGGCGTGT TTGTGCTCCT GGCCTGTGGC ACCATGTCCA GTACCTGTGG CCAGCTGGCC
AGCTACCCCC TGGCCCTAGT CAGGACCCGG ATGCAGGCGC AAGCCTCTAT TGAGGGCGCT
CCGGAGGTGA CCATGAGCAG CCTCTTCAAA CATATCCTGC GGACCGAGGG GGCCTTCGGG
CTGTACAGGG GGCTGGCCCC CAACTTCATG AAGGTCATCC CAGCTGTGAG CATCAGCTAC
GTGGTCTACG AGAACCTGAA GATCACCCTG GGCGTGCAGT CGCGGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005251745.1
CDS121..1668
Misc Feature(1)280..453(+)
Misc Feature(2)283..450(+)
Misc Feature(3)292..435(+)
Misc Feature(4)466..681(+)
Misc Feature(5)484..684(+)
Misc Feature(6)493..672(+)
Misc Feature(7)814..1080(+)
Misc Feature(8)1084..1362(+)
Misc Feature(9)1378..1650(+)
Translation
Position Chain Variation Link
26 26 c, g dbSNP:558528433
41 41 c, t dbSNP:555500539
52 52 a, t dbSNP:575317254
79 79 a, c dbSNP:768535577
83 83 c, t dbSNP:774409556
88 88 c, g, t dbSNP:748019680
90 90 g, t dbSNP:565635680
93 93 c, t dbSNP:760921541
105 105 c, g, t dbSNP:111769589
106 106 c, g, t dbSNP:112610099
118 118 c, t dbSNP:759934351
119 119 c, g dbSNP:765517194
127 127 a, g dbSNP:753307423
144 144 c, t dbSNP:534756011
158 158 c, t dbSNP:767084559
165 165 a, g dbSNP:749902845
167 167 -, cgc dbSNP:746100757
168 168 -, cgccgccgc dbSNP:781505525
173 173 c, t dbSNP:755662474
180 180 c, g dbSNP:779888347
183 183 c, t dbSNP:753532882
196 196 c, g dbSNP:754874456
200 200 c, t dbSNP:778512097
201 201 a, g dbSNP:748123141
227 227 a, g dbSNP:554321111
234 234 c, t dbSNP:777934320
235 235 a, g dbSNP:747145716
240 240 c, t dbSNP:770966841
243 243 c, t dbSNP:776906520
249 249 c, t dbSNP:759742189
251 251 c, t dbSNP:770209590
252 252 a, g dbSNP:775675174
255 255 c, t dbSNP:763537278
266 266 c, g dbSNP:764557683
274 274 a, g dbSNP:138886536
288 288 c, g dbSNP:752077892
291 291 c, t dbSNP:760327745
311 311 c, g dbSNP:765974011
312 312 c, g, t dbSNP:538130770
316 316 c, t dbSNP:754518150
322 322 a, g dbSNP:778801040
324 324 c, t dbSNP:752387197
353 353 a, g dbSNP:758190735
362 362 a, g dbSNP:777732029
367 367 c, g dbSNP:746948281
368 368 a, g dbSNP:771060504
371 371 g, t dbSNP:781193021
380 380 a, g dbSNP:746118191
414 414 a, t dbSNP:771289854
416 416 a, g dbSNP:776822595
439 439 a, g dbSNP:760114660
443 443 a, g dbSNP:148566637
454 454 c, g dbSNP:138425637
466 466 a, c dbSNP:373725272
472 472 a, t dbSNP:761463295
473 473 a, g dbSNP:201454954
476 476 -, ggtgtttaa dbSNP:760359903
480 480 -, t dbSNP:34104513
503 503 a, g dbSNP:750100888
504 504 a, t dbSNP:755784724
511 511 c, t dbSNP:35473740
512 512 a, g dbSNP:758293495
514 514 a, g dbSNP:777854337
519 519 c, t dbSNP:747143850
520 520 a, g dbSNP:757331567
521 521 c, t dbSNP:370288653
522 522 a, g, t dbSNP:558972121
534 534 a, g dbSNP:770053771
543 543 a, g dbSNP:775882793
544 544 a, c, t dbSNP:771182815
545 545 a, g dbSNP:539274578
547 547 a, g dbSNP:760327859
548 548 a, t dbSNP:144383132
549 549 c, t dbSNP:776161764
566 566 a, c, t dbSNP:759162104
567 567 c, t dbSNP:752441598
585 585 a, t dbSNP:758170699
586 586 a, g dbSNP:746022262
593 593 a, g dbSNP:751498139
602 602 a, g dbSNP:776167122
605 605 c, t dbSNP:759135831
606 606 a, g dbSNP:571994044
612 612 a, t dbSNP:542169131
634 634 a, g dbSNP:755406713
635 635 c, t dbSNP:752496326
645 645 c, t dbSNP:748634777
646 646 a, g dbSNP:772583995
650 650 c, t dbSNP:748220703
651 651 a, g dbSNP:146596005
653 653 c, t dbSNP:574131158
656 656 c, t dbSNP:769292052
657 657 c, g dbSNP:142703551
660 660 c, t dbSNP:7859709
661 661 a, c, g, t dbSNP:369537472
669 669 c, t dbSNP:767528105
670 670 a, g dbSNP:750322632
699 699 c, t dbSNP:576891746
700 700 a, g dbSNP:151146132
709 709 a, g dbSNP:781364472
714 714 c, g, t dbSNP:754116133
715 715 a, g dbSNP:765719372
718 718 a, g dbSNP:753156452
720 720 c, t dbSNP:758660440
724 724 c, t dbSNP:778414541
743 743 c, t dbSNP:747459916
764 764 a, g dbSNP:752115914
768 768 a, g dbSNP:201631831
770 770 c, t dbSNP:370988341
771 771 a, g dbSNP:748832966
774 774 c, t dbSNP:754478157
777 777 a, g dbSNP:201869990
795 795 a, g dbSNP:747696280
806 806 c, t dbSNP:771904007
807 807 g, t dbSNP:772842561
825 825 c, g dbSNP:557639053
826 826 a, c dbSNP:771041847
828 828 g, t dbSNP:776680157
852 852 c, t dbSNP:199564503
853 853 a, g dbSNP:765220171
854 854 c, t dbSNP:140086444
864 864 c, t dbSNP:763022200
869 869 c, t dbSNP:372542969
870 870 a, g dbSNP:771071856
872 872 c, g dbSNP:751914863
888 888 c, t dbSNP:762130155
893 893 c, t dbSNP:768077378
894 894 a, g dbSNP:750914983
916 916 c, g, t dbSNP:150336698
917 917 a, g dbSNP:372118470
921 921 -, caa dbSNP:749342875
924 924 a, c dbSNP:781070275
928 928 a, g dbSNP:745836070
929 929 g, t dbSNP:766359255
930 930 a, g dbSNP:769573706
935 935 c, t dbSNP:753817818
936 936 c, t dbSNP:375402805
937 937 a, g dbSNP:780007071
948 948 c, t dbSNP:749392034
949 949 a, g dbSNP:755086874
950 950 c, t dbSNP:369203375
952 952 a, c dbSNP:748301336
960 960 c, t dbSNP:373056648
962 962 a, g dbSNP:144936572
965 965 a, g dbSNP:761079227
969 969 a, c dbSNP:766835860
970 970 a, g dbSNP:542635849
976 976 a, g dbSNP:762430803
984 984 c, g dbSNP:376606350
988 988 c, t dbSNP:370591194
989 989 a, g dbSNP:201242773
995 995 a, g dbSNP:767446116
1004 1004 a, g dbSNP:146433346
1006 1006 a, g dbSNP:750278943
1020 1020 a, c, t dbSNP:755929446
1024 1024 a, g dbSNP:749188523
1026 1026 a, g dbSNP:755015821
1032 1032 c, t dbSNP:778922590
1037 1037 a, g dbSNP:544724772
1041 1041 c, t dbSNP:772329874
1050 1050 c, t dbSNP:773391642
1064 1064 a, g dbSNP:770459495
1072 1072 g, t dbSNP:556095628
1084 1084 a, g dbSNP:371529907
1087 1087 a, t dbSNP:771644764
1095 1095 a, g dbSNP:772756499
1101 1101 c, t dbSNP:760327411
1102 1102 a, g dbSNP:575643847
1111 1111 a, g dbSNP:753708155
1114 1114 a, g dbSNP:759304684
1119 1119 g, t dbSNP:765217256
1120 1120 g, t dbSNP:752625486
1122 1122 c, g dbSNP:758475931
1137 1137 c, t dbSNP:777849436
1138 1138 a, g dbSNP:751749247
1146 1146 c, t dbSNP:757458201
1159 1159 a, g dbSNP:781236718
1177 1177 c, t dbSNP:142204445
1178 1178 a, g dbSNP:201422874
1180 1180 a, t dbSNP:779266773
1184 1184 c, t dbSNP:370055267
1185 1185 a, g dbSNP:372688826
1189 1189 c, t dbSNP:776350434
1190 1190 a, g dbSNP:145988246
1203 1203 c, g dbSNP:140777921
1206 1206 c, t dbSNP:775223622
1216 1216 c, t dbSNP:762703146
1222 1222 c, t dbSNP:764138101
1224 1224 c, t dbSNP:569190005
1233 1233 g, t dbSNP:761962113
1235 1235 c, t dbSNP:200130106
1242 1242 c, g dbSNP:767442589
1245 1245 a, g dbSNP:750699865
1248 1248 a, c, g dbSNP:531778526
1249 1249 c, g, t dbSNP:754304263
1252 1252 a, g dbSNP:779540699
1257 1257 c, t dbSNP:748536660
1260 1260 a, c, g dbSNP:142900894
1265 1265 a, g dbSNP:745548531
1272 1272 c, t dbSNP:769473901
1277 1277 c, t dbSNP:775097320
1278 1278 c, t dbSNP:377362242
1292 1292 c, g dbSNP:768273167
1293 1293 c, t dbSNP:774362337
1296 1296 -, c dbSNP:773599471
1296 1296 c, t dbSNP:144905207
1297 1297 a, g dbSNP:767669209
1299 1299 c, t dbSNP:773467573
1309 1309 a, g dbSNP:760816369
1310 1310 a, c, g dbSNP:367599861
1314 1314 c, t dbSNP:571794213
1335 1335 a, g, t dbSNP:527757590
1347 1347 c, t dbSNP:148311002
1353 1353 a, g dbSNP:369981036
1359 1359 c, g dbSNP:764639834
1362 1362 c, t dbSNP:752053223
1375 1375 a, g dbSNP:755638307
1377 1377 a, g dbSNP:141401867
1378 1378 a, g dbSNP:753561842
1383 1383 c, t dbSNP:754630387
1384 1384 a, g dbSNP:778606816
1387 1387 a, c, g dbSNP:747954096
1392 1392 c, t dbSNP:542867942
1395 1395 a, g dbSNP:746883992
1404 1404 c, g, t dbSNP:771156426
1407 1407 c, t dbSNP:759628591
1414 1414 a, g dbSNP:769955139
1420 1420 a, g, t dbSNP:775766266
1446 1446 -, c dbSNP:770577173
1449 1449 c, g dbSNP:764372216
1467 1467 c, t dbSNP:752085981
1469 1469 a, g dbSNP:762271253
1477 1477 a, g dbSNP:767983433
1478 1478 c, t dbSNP:753341608
1479 1479 a, g dbSNP:547576840
1491 1491 c, t dbSNP:369405997
1496 1496 a, g dbSNP:751448393
1497 1497 c, t dbSNP:150859583
1500 1500 c, t dbSNP:781056301
1502 1502 c, t dbSNP:373230180
1503 1503 a, g dbSNP:16930267
1510 1510 a, g dbSNP:780145419
1517 1517 a, g dbSNP:749338871
1518 1518 a, c dbSNP:768918046
1521 1521 c, t dbSNP:371151024
1535 1535 c, t dbSNP:201558106
1540 1540 c, t dbSNP:374458248
1541 1541 a, g dbSNP:772416779
1543 1543 a, g dbSNP:773606820
1553 1553 c, t dbSNP:142830945
1555 1555 c, t dbSNP:554406399
1557 1557 c, t dbSNP:775178382
1567 1567 a, g dbSNP:762527775
1582 1582 a, c dbSNP:763894403
1586 1586 a, t dbSNP:375698904
1588 1588 a, g dbSNP:761513312
1589 1589 c, t dbSNP:767280977
1599 1599 a, c dbSNP:750361886
1612 1612 a, g dbSNP:756121970
1619 1619 a, g dbSNP:779836096
1620 1620 c, t dbSNP:111440458
1621 1621 a, g dbSNP:755047936
1626 1626 c, t dbSNP:779114460
1629 1629 c, t dbSNP:748311616
1630 1630 a, g dbSNP:146124484
1642 1642 a, g dbSNP:772346771
1644 1644 c, t dbSNP:778158911
1647 1647 c, t dbSNP:747304394
1648 1648 c, g dbSNP:771515643
1651 1651 a, g dbSNP:777263284
1653 1653 c, t dbSNP:762615145
1654 1654 a, g dbSNP:370283153
1662 1662 a, g dbSNP:774155897
1664 1664 a, g dbSNP:199739089
1669 1669 -, g dbSNP:759027068
1669 1669 c, t dbSNP:369962505
1670 1670 a, g, t dbSNP:201844320
1670 1670 -, g dbSNP:765268245
1672 1672 a, c, g, t dbSNP:200194334
1675 1675 a, g, t dbSNP:201248373
1676 1676 a, c, g dbSNP:577175137
1681 1681 c, t dbSNP:752894376
1682 1682 a, g dbSNP:377049698
1685 1685 a, c, t dbSNP:370271137
1686 1686 a, g dbSNP:374588749
1698 1698 a, g dbSNP:781764705
1699 1699 c, g dbSNP:746317417
1711 1711 c, t dbSNP:768300868
1712 1712 a, g dbSNP:199878817
1716 1716 a, c, g, t dbSNP:775510321
1717 1717 c, t dbSNP:760523646
1718 1718 c, t dbSNP:141910331
1741 1741 g, t dbSNP:547868115
1768 1768 c, t dbSNP:561255124
1777 1777 c, t dbSNP:530300344
1795 1795 c, t dbSNP:745933780
1799 1799 c, t dbSNP:770024989
1800 1800 a, g dbSNP:75511005
1822 1822 a, g dbSNP:186440107
1824 1824 a, c dbSNP:760534129
1840 1840 a, c dbSNP:532623293
1843 1843 c, t dbSNP:13298797
1845 1845 a, g dbSNP:552462679
1864 1864 a, g dbSNP:565329443
1875 1875 c, g, t dbSNP:114790720
1922 1922 c, t dbSNP:541860686
1928 1928 c, t dbSNP:780375217
1939 1939 a, g dbSNP:372694601
1968 1968 c, t dbSNP:7872702
1969 1969 a, g dbSNP:140738643
1987 1987 c, t dbSNP:577113755
2007 2007 c, t dbSNP:374091374
2032 2032 c, t dbSNP:10987876
2033 2033 a, c, g dbSNP:188541776
2054 2054 c, t dbSNP:369881030
2055 2055 a, g dbSNP:111828101
2058 2058 c, g dbSNP:572383902
2078 2078 -, ctgc dbSNP:567835255
2085 2085 a, c dbSNP:750834588
2097 2097 a, g dbSNP:541101417
2124 2124 a, c dbSNP:376171587
2163 2163 a, c dbSNP:369199483
2181 2181 c, g dbSNP:561350806
2203 2203 c, g dbSNP:3814524
2216 2216 a, g dbSNP:760444702
2267 2267 c, t dbSNP:767020937
2280 2280 c, t dbSNP:543515646
2324 2324 c, t dbSNP:564699238
2351 2351 -, g dbSNP:34224940
2401 2401 a, g dbSNP:770879666
2419 2419 a, c dbSNP:757971886
2429 2429 c, t dbSNP:563501308
2436 2436 a, g dbSNP:532609821
2448 2448 a, g dbSNP:552446244
2474 2474 c, t dbSNP:566269410
2475 2475 a, g dbSNP:746741415
2480 2480 a, g dbSNP:756864174
2483 2483 c, g dbSNP:528071747
2486 2486 a, c dbSNP:116513398
2495 2495 a, g dbSNP:180947406
2525 2525 a, g dbSNP:537095783
2526 2526 a, g dbSNP:185116755
2529 2529 g, t dbSNP:745683615
2574 2574 c, t dbSNP:57052978
2576 2576 a, g dbSNP:769689731
2612 2612 c, t dbSNP:147539248
2613 2613 -, gag dbSNP:567976888
2615 2615 a, g dbSNP:775457386
2655 2655 a, c, t dbSNP:749315548
2683 2683 c, t dbSNP:139384794
2690 2690 a, g dbSNP:536517546
2767 2767 a, t dbSNP:774303940
2770 2770 -, ctaa dbSNP:528213954
2771 2771 -, ctaa dbSNP:777101904
2774 2774 -, ctaa dbSNP:773891780
2786 2786 c, g dbSNP:542129619
2821 2821 c, t dbSNP:191060669
2912 2912 c, t dbSNP:764745985
2936 2936 c, g dbSNP:767657703
2967 2967 c, t dbSNP:773668033
2968 2968 a, g dbSNP:574788142
2993 2993 -, t dbSNP:35604217
3046 3046 a, t dbSNP:537978043
3058 3058 c, t dbSNP:761158288
3059 3059 a, g dbSNP:766965728
3090 3090 g, t dbSNP:374692425
3116 3116 c, t dbSNP:532543390
3142 3142 c, t dbSNP:546018894
3156 3156 a, t dbSNP:559910298
3185 3185 c, t dbSNP:528751634
3196 3196 a, g dbSNP:757840839
3217 3217 c, g dbSNP:369941858
3220 3220 a, c, g dbSNP:10760538
3222 3222 -, g dbSNP:35358699
3359 3359 c, t dbSNP:561483406
3388 3388 a, g dbSNP:35037027
3397 3397 a, c dbSNP:751043182
3417 3417 c, t dbSNP:530560615
3425 3425 a, g dbSNP:184011703
3463 3463 a, g dbSNP:77244294

Target ORF information:

RefSeq Version XM_005251688
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005251688

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGTGAGCA GTGTGTTGTG CCGCTGTGTG GCCTCCCCGC CGCCGGACGC CGCCGCCACC 
GCCGCCTCTT CGTCTGCCTC ATCGCCGGCG TCCGTGGGGG ACCCCTGCGG CGGCGCTATC
TGCGGGGGCC CGGACCACCG GCTGCGCCTG TGGAGACTCT TTCAGACGCT CGACGTCAAC
CGGGACGGCG GCCTGTGTGT CAACGACCTG GCGGTGGGGC TGCGGCGCCT GGGACTGCAC
CGCACCGAGG GCGAGCTCCA GAAAATTGTA CAAGCTGGAG ATAAGGACCT TGATGGGCAG
CTAGACTTTG AAGAATTTGT CCATTATCTC CAAGATCATG AGAAGAAGCT GAGGCTGGTG
TTTAAGAGTT TGGACAAAAA GAATGATGGA CGCATTGACG CGCAGGAGAT CATGCAGTCC
CTGCGGGACT TGGGAGTCAA GATATCTGAA CAGCAGGCAG AAAAAATTCT CAAGAGAATA
CGAACGGGCC ATTTCTGGGG CCCTGTCACC TACATGGATA AAAACGGCAC GATGACCATT
GACTGGAACG AGTGGAGAGA CTACCACCTC CTCCACCCCG TGGAAAACAT CCCCGAGATC
ATCCTCTACT GGAAGCATTC CACGATCTTT GATGTGGGTG AGAATCTAAC GGTCCCGGAT
GAGTTCACAG TGGAGGAGAG GCAGACGGGG ATGTGGTGGA GACACCTGGT GGCAGGAGGT
GGGGCAGGGG CCGTATCCAG AACCTGCACG GCCCCCCTGG ACAGGCTCAA GGTGCTCATG
CAGGTCCATG CCTCCCGCAG CAACAACATG GGCATCGTTG GTGGCTTCAC TCAGATGATT
CGAGAAGGAG GGGCCAGGTC ACTCTGGCGG GGCAATGGCA TCAACGTCCT CAAAATTGCC
CCCGAATCAG CCATCAAATT CATGGCCTAT GAGCAGATCA AGCGCCTTGT TGGTAGTGAC
CAGGAGACTC TGAGGATTCA CGAGAGGCTT GTGGCAGGGT CCTTGGCAGG GGCCATCGCC
CAGAGCAGCA TCTACCCAAT GGAGGTCCTG AAGACCCGGA TGGCGCTGCG GAAGACAGGC
CAGTACTCAG GAATGCTGGA CTGCGCCAGG AGGATCCTGG CCAGAGAGGG GGTGGCCGCC
TTCTACAAAG GCTATGTCCC CAACATGCTG GGCATCATCC CCTATGCCGG CATCGACCTT
GCAGTCTACG AGACGCTCAA GAATGCCTGG CTGCAGCACT ATGCAGTGAA CAGCGCGGAC
CCCGGCGTGT TTGTGCTCCT GGCCTGTGGC ACCATGTCCA GTACCTGTGG CCAGCTGGCC
AGCTACCCCC TGGCCCTAGT CAGGACCCGG ATGCAGGCGC AAGCCTCTAT TGAGGGCGCT
CCGGAGGTGA CCATGAGCAG CCTCTTCAAA CATATCCTGC GGACCGAGGG GGCCTTCGGG
CTGTACAGGG GGCTGGCCCC CAACTTCATG AAGGTCATCC CAGCTGTGAG CATCAGCTAC
GTGGTCTACG AGAACCTGAA GATCACCCTG GGCGTGCAGT CGCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu42964
Accession Version XM_006716948.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1446bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product calcium-binding mitochondrial carrier protein SCaMC-2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008470.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCTCTGTC TGTGCCTGTA TGTGCCGGTC ATCGGGGAAG CCCAGACCGA GTTCCAGTAC 
TTTGAGTCGA AGGGGCTCCC TGCCGAGCTG AAGTCCATTT TCAAGCTCAG TGTCTTCATC
CCCTCCCAGG AATTCTCCAC CTACCGCCAG TGGAAGCAGA AAATTGTACA AGCTGGAGAT
AAGGACCTTG ATGGGCAGCT AGACTTTGAA GAATTTGTCC ATTATCTCCA AGATCATGAG
AAGAAGCTGA GGCTGGTGTT TAAGAGTTTG GACAAAAAGA ATGATGGACG CATTGACGCG
CAGGAGATCA TGCAGTCCCT GCGGGACTTG GGAGTCAAGA TATCTGAACA GCAGGCAGAA
AAAATTCTCA AGAGAATACG AACGGGCCAT TTCTGGGGCC CTGTCACCTA CATGGATAAA
AACGGCACGA TGACCATTGA CTGGAACGAG TGGAGAGACT ACCACCTCCT CCACCCCGTG
GAAAACATCC CCGAGATCAT CCTCTACTGG AAGCATTCCA CGATCTTTGA TGTGGGTGAG
AATCTAACGG TCCCGGATGA GTTCACAGTG GAGGAGAGGC AGACGGGGAT GTGGTGGAGA
CACCTGGTGG CAGGAGGTGG GGCAGGGGCC GTATCCAGAA CCTGCACGGC CCCCCTGGAC
AGGCTCAAGG TGCTCATGCA GGTCCATGCC TCCCGCAGCA ACAACATGGG CATCGTTGGT
GGCTTCACTC AGATGATTCG AGAAGGAGGG GCCAGGTCAC TCTGGCGGGG CAATGGCATC
AACGTCCTCA AAATTGCCCC CGAATCAGCC ATCAAATTCA TGGCCTATGA GCAGATCAAG
CGCCTTGTTG GTAGTGACCA GGAGACTCTG AGGATTCACG AGAGGCTTGT GGCAGGGTCC
TTGGCAGGGG CCATCGCCCA GAGCAGCATC TACCCAATGG AGGTCCTGAA GACCCGGATG
GCGCTGCGGA AGACAGGCCA GTACTCAGGA ATGCTGGACT GCGCCAGGAG GATCCTGGCC
AGAGAGGGGG TGGCCGCCTT CTACAAAGGC TATGTCCCCA ACATGCTGGG CATCATCCCC
TATGCCGGCA TCGACCTTGC AGTCTACGAG ACGCTCAAGA ATGCCTGGCT GCAGCACTAT
GCAGTGAACA GCGCGGACCC CGGCGTGTTT GTGCTCCTGG CCTGTGGCAC CATGTCCAGT
ACCTGTGGCC AGCTGGCCAG CTACCCCCTG GCCCTAGTCA GGACCCGGAT GCAGGCGCAA
GCCTCTATTG AGGGCGCTCC GGAGGTGACC ATGAGCAGCC TCTTCAAACA TATCCTGCGG
ACCGAGGGGG CCTTCGGGCT GTACAGGGGG CTGGCCCCCA ACTTCATGAA GGTCATCCCA
GCTGTGAGCA TCAGCTACGT GGTCTACGAG AACCTGAAGA TCACCCTGGG CGTGCAGTCG
CGGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006717011.1
CDS259..1704
Misc Feature(1)343..489(+)
Misc Feature(2)367..471(+)
Misc Feature(3)502..717(+)
Misc Feature(4)520..720(+)
Misc Feature(5)529..708(+)
Misc Feature(6)850..1116(+)
Misc Feature(7)1120..1398(+)
Misc Feature(8)1414..1686(+)
Translation
Position Chain Variation Link
3 3 a, g dbSNP:537683527
48 48 c, t dbSNP:7856792
54 54 c, t dbSNP:534863013
67 67 g, t dbSNP:546161349
89 89 c, t dbSNP:768595036
121 121 c, t dbSNP:552912525
126 126 g, t dbSNP:774366039
138 138 c, g dbSNP:75132622
140 140 g, t dbSNP:192815286
168 168 c, g dbSNP:41276222
189 189 c, t dbSNP:561889641
190 190 a, g dbSNP:750704925
195 195 c, t dbSNP:760932370
203 203 c, t dbSNP:766916465
217 217 -, c dbSNP:749801009
220 220 c, t dbSNP:367597539
227 227 c, g dbSNP:116892997
235 235 c, t dbSNP:768165226
243 243 a, c dbSNP:374851612
244 244 c, t dbSNP:747828767
250 250 g, t dbSNP:139240119
252 252 c, t dbSNP:565636563
253 253 a, g dbSNP:372587321
262 262 a, c dbSNP:746861991
263 263 c, t dbSNP:564787611
267 267 c, t dbSNP:776767949
269 269 -, tgtgcctgta dbSNP:769170122
274 274 c, t dbSNP:759489229
276 276 a, g, t dbSNP:765416604
279 279 -, tgtgc dbSNP:774725023
279 279 c, t dbSNP:947624
280 280 a, g dbSNP:764414142
284 284 c, t dbSNP:547339694
285 285 a, g dbSNP:114706110
287 287 c, t dbSNP:767712754
288 288 c, t dbSNP:528904604
290 290 c, t dbSNP:756572966
291 291 c, t dbSNP:150615663
292 292 a, g dbSNP:375511161
307 307 a, g dbSNP:757816321
309 309 c, g dbSNP:568904522
314 314 a, g dbSNP:746629576
315 315 a, g dbSNP:770773776
316 316 c, t dbSNP:776506321
319 319 c, t dbSNP:745836152
321 321 c, t dbSNP:769808180
326 326 c, t dbSNP:377289085
327 327 a, g dbSNP:139670262
333 333 a, g dbSNP:370177594
335 335 c, t dbSNP:764064683
336 336 c, t dbSNP:149387961
342 342 c, t dbSNP:779581284
354 354 c, t dbSNP:767899543
356 356 c, t dbSNP:750737186
362 362 a, g dbSNP:756446179
364 364 c, t dbSNP:750991481
377 377 -, tccc dbSNP:762111328
377 377 a, t dbSNP:754339466
380 380 a, c dbSNP:757986323
381 381 -, tgggagggg dbSNP:771720766
393 393 a, c dbSNP:374358411
394 394 c, t dbSNP:79935560
397 397 a, g dbSNP:746679750
400 400 c, t dbSNP:74398782
403 403 c, t dbSNP:144731034
404 404 a, g dbSNP:202228736
416 416 a, g dbSNP:745709560
450 450 a, t dbSNP:771289854
452 452 a, g dbSNP:776822595
475 475 a, g dbSNP:760114660
479 479 a, g dbSNP:148566637
490 490 c, g dbSNP:138425637
502 502 a, c dbSNP:373725272
508 508 a, t dbSNP:761463295
509 509 a, g dbSNP:201454954
512 512 -, ggtgtttaa dbSNP:760359903
516 516 -, t dbSNP:34104513
539 539 a, g dbSNP:750100888
540 540 a, t dbSNP:755784724
547 547 c, t dbSNP:35473740
548 548 a, g dbSNP:758293495
550 550 a, g dbSNP:777854337
555 555 c, t dbSNP:747143850
556 556 a, g dbSNP:757331567
557 557 c, t dbSNP:370288653
558 558 a, g, t dbSNP:558972121
570 570 a, g dbSNP:770053771
579 579 a, g dbSNP:775882793
580 580 a, c, t dbSNP:771182815
581 581 a, g dbSNP:539274578
583 583 a, g dbSNP:760327859
584 584 a, t dbSNP:144383132
585 585 c, t dbSNP:776161764
602 602 a, c, t dbSNP:759162104
603 603 c, t dbSNP:752441598
621 621 a, t dbSNP:758170699
622 622 a, g dbSNP:746022262
629 629 a, g dbSNP:751498139
638 638 a, g dbSNP:776167122
641 641 c, t dbSNP:759135831
642 642 a, g dbSNP:571994044
648 648 a, t dbSNP:542169131
670 670 a, g dbSNP:755406713
671 671 c, t dbSNP:752496326
681 681 c, t dbSNP:748634777
682 682 a, g dbSNP:772583995
686 686 c, t dbSNP:748220703
687 687 a, g dbSNP:146596005
689 689 c, t dbSNP:574131158
692 692 c, t dbSNP:769292052
693 693 c, g dbSNP:142703551
696 696 c, t dbSNP:7859709
697 697 a, c, g, t dbSNP:369537472
705 705 c, t dbSNP:767528105
706 706 a, g dbSNP:750322632
735 735 c, t dbSNP:576891746
736 736 a, g dbSNP:151146132
745 745 a, g dbSNP:781364472
750 750 c, g, t dbSNP:754116133
751 751 a, g dbSNP:765719372
754 754 a, g dbSNP:753156452
756 756 c, t dbSNP:758660440
760 760 c, t dbSNP:778414541
779 779 c, t dbSNP:747459916
800 800 a, g dbSNP:752115914
804 804 a, g dbSNP:201631831
806 806 c, t dbSNP:370988341
807 807 a, g dbSNP:748832966
810 810 c, t dbSNP:754478157
813 813 a, g dbSNP:201869990
831 831 a, g dbSNP:747696280
842 842 c, t dbSNP:771904007
843 843 g, t dbSNP:772842561
861 861 c, g dbSNP:557639053
862 862 a, c dbSNP:771041847
864 864 g, t dbSNP:776680157
888 888 c, t dbSNP:199564503
889 889 a, g dbSNP:765220171
890 890 c, t dbSNP:140086444
900 900 c, t dbSNP:763022200
905 905 c, t dbSNP:372542969
906 906 a, g dbSNP:771071856
908 908 c, g dbSNP:751914863
924 924 c, t dbSNP:762130155
929 929 c, t dbSNP:768077378
930 930 a, g dbSNP:750914983
952 952 c, g, t dbSNP:150336698
953 953 a, g dbSNP:372118470
957 957 -, caa dbSNP:749342875
960 960 a, c dbSNP:781070275
964 964 a, g dbSNP:745836070
965 965 g, t dbSNP:766359255
966 966 a, g dbSNP:769573706
971 971 c, t dbSNP:753817818
972 972 c, t dbSNP:375402805
973 973 a, g dbSNP:780007071
984 984 c, t dbSNP:749392034
985 985 a, g dbSNP:755086874
986 986 c, t dbSNP:369203375
988 988 a, c dbSNP:748301336
996 996 c, t dbSNP:373056648
998 998 a, g dbSNP:144936572
1001 1001 a, g dbSNP:761079227
1005 1005 a, c dbSNP:766835860
1006 1006 a, g dbSNP:542635849
1012 1012 a, g dbSNP:762430803
1020 1020 c, g dbSNP:376606350
1024 1024 c, t dbSNP:370591194
1025 1025 a, g dbSNP:201242773
1031 1031 a, g dbSNP:767446116
1040 1040 a, g dbSNP:146433346
1042 1042 a, g dbSNP:750278943
1056 1056 a, c, t dbSNP:755929446
1060 1060 a, g dbSNP:749188523
1062 1062 a, g dbSNP:755015821
1068 1068 c, t dbSNP:778922590
1073 1073 a, g dbSNP:544724772
1077 1077 c, t dbSNP:772329874
1086 1086 c, t dbSNP:773391642
1100 1100 a, g dbSNP:770459495
1108 1108 g, t dbSNP:556095628
1120 1120 a, g dbSNP:371529907
1123 1123 a, t dbSNP:771644764
1131 1131 a, g dbSNP:772756499
1137 1137 c, t dbSNP:760327411
1138 1138 a, g dbSNP:575643847
1147 1147 a, g dbSNP:753708155
1150 1150 a, g dbSNP:759304684
1155 1155 g, t dbSNP:765217256
1156 1156 g, t dbSNP:752625486
1158 1158 c, g dbSNP:758475931
1173 1173 c, t dbSNP:777849436
1174 1174 a, g dbSNP:751749247
1182 1182 c, t dbSNP:757458201
1195 1195 a, g dbSNP:781236718
1213 1213 c, t dbSNP:142204445
1214 1214 a, g dbSNP:201422874
1216 1216 a, t dbSNP:779266773
1220 1220 c, t dbSNP:370055267
1221 1221 a, g dbSNP:372688826
1225 1225 c, t dbSNP:776350434
1226 1226 a, g dbSNP:145988246
1239 1239 c, g dbSNP:140777921
1242 1242 c, t dbSNP:775223622
1252 1252 c, t dbSNP:762703146
1258 1258 c, t dbSNP:764138101
1260 1260 c, t dbSNP:569190005
1269 1269 g, t dbSNP:761962113
1271 1271 c, t dbSNP:200130106
1278 1278 c, g dbSNP:767442589
1281 1281 a, g dbSNP:750699865
1284 1284 a, c, g dbSNP:531778526
1285 1285 c, g, t dbSNP:754304263
1288 1288 a, g dbSNP:779540699
1293 1293 c, t dbSNP:748536660
1296 1296 a, c, g dbSNP:142900894
1301 1301 a, g dbSNP:745548531
1308 1308 c, t dbSNP:769473901
1313 1313 c, t dbSNP:775097320
1314 1314 c, t dbSNP:377362242
1328 1328 c, g dbSNP:768273167
1329 1329 c, t dbSNP:774362337
1332 1332 -, c dbSNP:773599471
1332 1332 c, t dbSNP:144905207
1333 1333 a, g dbSNP:767669209
1335 1335 c, t dbSNP:773467573
1345 1345 a, g dbSNP:760816369
1346 1346 a, c, g dbSNP:367599861
1350 1350 c, t dbSNP:571794213
1371 1371 a, g, t dbSNP:527757590
1383 1383 c, t dbSNP:148311002
1389 1389 a, g dbSNP:369981036
1395 1395 c, g dbSNP:764639834
1398 1398 c, t dbSNP:752053223
1411 1411 a, g dbSNP:755638307
1413 1413 a, g dbSNP:141401867
1414 1414 a, g dbSNP:753561842
1419 1419 c, t dbSNP:754630387
1420 1420 a, g dbSNP:778606816
1423 1423 a, c, g dbSNP:747954096
1428 1428 c, t dbSNP:542867942
1431 1431 a, g dbSNP:746883992
1440 1440 c, g, t dbSNP:771156426
1443 1443 c, t dbSNP:759628591
1450 1450 a, g dbSNP:769955139
1456 1456 a, g, t dbSNP:775766266
1482 1482 -, c dbSNP:770577173
1485 1485 c, g dbSNP:764372216
1503 1503 c, t dbSNP:752085981
1505 1505 a, g dbSNP:762271253
1513 1513 a, g dbSNP:767983433
1514 1514 c, t dbSNP:753341608
1515 1515 a, g dbSNP:547576840
1527 1527 c, t dbSNP:369405997
1532 1532 a, g dbSNP:751448393
1533 1533 c, t dbSNP:150859583
1536 1536 c, t dbSNP:781056301
1538 1538 c, t dbSNP:373230180
1539 1539 a, g dbSNP:16930267
1546 1546 a, g dbSNP:780145419
1553 1553 a, g dbSNP:749338871
1554 1554 a, c dbSNP:768918046
1557 1557 c, t dbSNP:371151024
1571 1571 c, t dbSNP:201558106
1576 1576 c, t dbSNP:374458248
1577 1577 a, g dbSNP:772416779
1579 1579 a, g dbSNP:773606820
1589 1589 c, t dbSNP:142830945
1591 1591 c, t dbSNP:554406399
1593 1593 c, t dbSNP:775178382
1603 1603 a, g dbSNP:762527775
1618 1618 a, c dbSNP:763894403
1622 1622 a, t dbSNP:375698904
1624 1624 a, g dbSNP:761513312
1625 1625 c, t dbSNP:767280977
1635 1635 a, c dbSNP:750361886
1648 1648 a, g dbSNP:756121970
1655 1655 a, g dbSNP:779836096
1656 1656 c, t dbSNP:111440458
1657 1657 a, g dbSNP:755047936
1662 1662 c, t dbSNP:779114460
1665 1665 c, t dbSNP:748311616
1666 1666 a, g dbSNP:146124484
1678 1678 a, g dbSNP:772346771
1680 1680 c, t dbSNP:778158911
1683 1683 c, t dbSNP:747304394
1684 1684 c, g dbSNP:771515643
1687 1687 a, g dbSNP:777263284
1689 1689 c, t dbSNP:762615145
1690 1690 a, g dbSNP:370283153
1698 1698 a, g dbSNP:774155897
1700 1700 a, g dbSNP:199739089
1705 1705 -, g dbSNP:759027068
1705 1705 c, t dbSNP:369962505
1706 1706 a, g, t dbSNP:201844320
1706 1706 -, g dbSNP:765268245
1708 1708 a, c, g, t dbSNP:200194334
1711 1711 a, g, t dbSNP:201248373
1712 1712 a, c, g dbSNP:577175137
1717 1717 c, t dbSNP:752894376
1718 1718 a, g dbSNP:377049698
1721 1721 a, c, t dbSNP:370271137
1722 1722 a, g dbSNP:374588749
1734 1734 a, g dbSNP:781764705
1735 1735 c, g dbSNP:746317417
1747 1747 c, t dbSNP:768300868
1748 1748 a, g dbSNP:199878817
1752 1752 a, c, g, t dbSNP:775510321
1753 1753 c, t dbSNP:760523646
1754 1754 c, t dbSNP:141910331
1777 1777 g, t dbSNP:547868115
1804 1804 c, t dbSNP:561255124
1813 1813 c, t dbSNP:530300344
1831 1831 c, t dbSNP:745933780
1835 1835 c, t dbSNP:770024989
1836 1836 a, g dbSNP:75511005
1858 1858 a, g dbSNP:186440107
1860 1860 a, c dbSNP:760534129
1876 1876 a, c dbSNP:532623293
1879 1879 c, t dbSNP:13298797
1881 1881 a, g dbSNP:552462679
1900 1900 a, g dbSNP:565329443
1911 1911 c, g, t dbSNP:114790720
1958 1958 c, t dbSNP:541860686
1964 1964 c, t dbSNP:780375217
1975 1975 a, g dbSNP:372694601
2004 2004 c, t dbSNP:7872702
2005 2005 a, g dbSNP:140738643
2023 2023 c, t dbSNP:577113755
2043 2043 c, t dbSNP:374091374
2068 2068 c, t dbSNP:10987876
2069 2069 a, c, g dbSNP:188541776
2090 2090 c, t dbSNP:369881030
2091 2091 a, g dbSNP:111828101
2094 2094 c, g dbSNP:572383902
2114 2114 -, ctgc dbSNP:567835255
2121 2121 a, c dbSNP:750834588
2133 2133 a, g dbSNP:541101417
2160 2160 a, c dbSNP:376171587
2199 2199 a, c dbSNP:369199483
2217 2217 c, g dbSNP:561350806
2239 2239 c, g dbSNP:3814524
2252 2252 a, g dbSNP:760444702
2303 2303 c, t dbSNP:767020937
2316 2316 c, t dbSNP:543515646
2360 2360 c, t dbSNP:564699238
2387 2387 -, g dbSNP:34224940
2437 2437 a, g dbSNP:770879666
2455 2455 a, c dbSNP:757971886
2465 2465 c, t dbSNP:563501308
2472 2472 a, g dbSNP:532609821
2484 2484 a, g dbSNP:552446244
2510 2510 c, t dbSNP:566269410
2511 2511 a, g dbSNP:746741415
2516 2516 a, g dbSNP:756864174
2519 2519 c, g dbSNP:528071747
2522 2522 a, c dbSNP:116513398
2531 2531 a, g dbSNP:180947406
2561 2561 a, g dbSNP:537095783
2562 2562 a, g dbSNP:185116755
2565 2565 g, t dbSNP:745683615
2610 2610 c, t dbSNP:57052978
2612 2612 a, g dbSNP:769689731
2648 2648 c, t dbSNP:147539248
2649 2649 -, gag dbSNP:567976888
2651 2651 a, g dbSNP:775457386
2691 2691 a, c, t dbSNP:749315548
2719 2719 c, t dbSNP:139384794
2726 2726 a, g dbSNP:536517546
2803 2803 a, t dbSNP:774303940
2806 2806 -, ctaa dbSNP:528213954
2807 2807 -, ctaa dbSNP:777101904
2810 2810 -, ctaa dbSNP:773891780
2822 2822 c, g dbSNP:542129619
2857 2857 c, t dbSNP:191060669
2948 2948 c, t dbSNP:764745985
2972 2972 c, g dbSNP:767657703
3003 3003 c, t dbSNP:773668033
3004 3004 a, g dbSNP:574788142
3029 3029 -, t dbSNP:35604217
3082 3082 a, t dbSNP:537978043
3094 3094 c, t dbSNP:761158288
3095 3095 a, g dbSNP:766965728
3126 3126 g, t dbSNP:374692425
3152 3152 c, t dbSNP:532543390
3178 3178 c, t dbSNP:546018894
3192 3192 a, t dbSNP:559910298
3221 3221 c, t dbSNP:528751634
3232 3232 a, g dbSNP:757840839
3253 3253 c, g dbSNP:369941858
3256 3256 a, c, g dbSNP:10760538
3258 3258 -, g dbSNP:35358699
3395 3395 c, t dbSNP:561483406
3424 3424 a, g dbSNP:35037027
3433 3433 a, c dbSNP:751043182
3453 3453 c, t dbSNP:530560615
3461 3461 a, g dbSNP:184011703
3499 3499 a, g dbSNP:77244294

Target ORF information:

RefSeq Version XM_006716948
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006716948

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCTCTGTC TGTGCCTGTA TGTGCCGGTC ATCGGGGAAG CCCAGACCGA GTTCCAGTAC 
TTTGAGTCGA AGGGGCTCCC TGCCGAGCTG AAGTCCATTT TCAAGCTCAG TGTCTTCATC
CCCTCCCAGG AATTCTCCAC CTACCGCCAG TGGAAGCAGA AAATTGTACA AGCTGGAGAT
AAGGACCTTG ATGGGCAGCT AGACTTTGAA GAATTTGTCC ATTATCTCCA AGATCATGAG
AAGAAGCTGA GGCTGGTGTT TAAGAGTTTG GACAAAAAGA ATGATGGACG CATTGACGCG
CAGGAGATCA TGCAGTCCCT GCGGGACTTG GGAGTCAAGA TATCTGAACA GCAGGCAGAA
AAAATTCTCA AGAGAATACG AACGGGCCAT TTCTGGGGCC CTGTCACCTA CATGGATAAA
AACGGCACGA TGACCATTGA CTGGAACGAG TGGAGAGACT ACCACCTCCT CCACCCCGTG
GAAAACATCC CCGAGATCAT CCTCTACTGG AAGCATTCCA CGATCTTTGA TGTGGGTGAG
AATCTAACGG TCCCGGATGA GTTCACAGTG GAGGAGAGGC AGACGGGGAT GTGGTGGAGA
CACCTGGTGG CAGGAGGTGG GGCAGGGGCC GTATCCAGAA CCTGCACGGC CCCCCTGGAC
AGGCTCAAGG TGCTCATGCA GGTCCATGCC TCCCGCAGCA ACAACATGGG CATCGTTGGT
GGCTTCACTC AGATGATTCG AGAAGGAGGG GCCAGGTCAC TCTGGCGGGG CAATGGCATC
AACGTCCTCA AAATTGCCCC CGAATCAGCC ATCAAATTCA TGGCCTATGA GCAGATCAAG
CGCCTTGTTG GTAGTGACCA GGAGACTCTG AGGATTCACG AGAGGCTTGT GGCAGGGTCC
TTGGCAGGGG CCATCGCCCA GAGCAGCATC TACCCAATGG AGGTCCTGAA GACCCGGATG
GCGCTGCGGA AGACAGGCCA GTACTCAGGA ATGCTGGACT GCGCCAGGAG GATCCTGGCC
AGAGAGGGGG TGGCCGCCTT CTACAAAGGC TATGTCCCCA ACATGCTGGG CATCATCCCC
TATGCCGGCA TCGACCTTGC AGTCTACGAG ACGCTCAAGA ATGCCTGGCT GCAGCACTAT
GCAGTGAACA GCGCGGACCC CGGCGTGTTT GTGCTCCTGG CCTGTGGCAC CATGTCCAGT
ACCTGTGGCC AGCTGGCCAG CTACCCCCTG GCCCTAGTCA GGACCCGGAT GCAGGCGCAA
GCCTCTATTG AGGGCGCTCC GGAGGTGACC ATGAGCAGCC TCTTCAAACA TATCCTGCGG
ACCGAGGGGG CCTTCGGGCT GTACAGGGGG CTGGCCCCCA ACTTCATGAA GGTCATCCCA
GCTGTGAGCA TCAGCTACGT GGTCTACGAG AACCTGAAGA TCACCCTGGG CGTGCAGTCG
CGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu42965
Accession Version XM_006716949.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1137bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product calcium-binding mitochondrial carrier protein SCaMC-2 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008470.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578817036. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGTCCC TGCGGGACTT GGGAGTCAAG ATATCTGAAC AGCAGGCAGA AAAAATTCTC 
AAGAGAATAC GAACGGGCCA TTTCTGGGGC CCTGTCACCT ACATGGATAA AAACGGCACG
ATGACCATTG ACTGGAACGA GTGGAGAGAC TACCACCTCC TCCACCCCGT GGAAAACATC
CCCGAGATCA TCCTCTACTG GAAGCATTCC ACGATCTTTG ATGTGGGTGA GAATCTAACG
GTCCCGGATG AGTTCACAGT GGAGGAGAGG CAGACGGGGA TGTGGTGGAG ACACCTGGTG
GCAGGAGGTG GGGCAGGGGC CGTATCCAGA ACCTGCACGG CCCCCCTGGA CAGGCTCAAG
GTGCTCATGC AGGTCCATGC CTCCCGCAGC AACAACATGG GCATCGTTGG TGGCTTCACT
CAGATGATTC GAGAAGGAGG GGCCAGGTCA CTCTGGCGGG GCAATGGCAT CAACGTCCTC
AAAATTGCCC CCGAATCAGC CATCAAATTC ATGGCCTATG AGCAGATCAA GCGCCTTGTT
GGTAGTGACC AGGAGACTCT GAGGATTCAC GAGAGGCTTG TGGCAGGGTC CTTGGCAGGG
GCCATCGCCC AGAGCAGCAT CTACCCAATG GAGGTCCTGA AGACCCGGAT GGCGCTGCGG
AAGACAGGCC AGTACTCAGG AATGCTGGAC TGCGCCAGGA GGATCCTGGC CAGAGAGGGG
GTGGCCGCCT TCTACAAAGG CTATGTCCCC AACATGCTGG GCATCATCCC CTATGCCGGC
ATCGACCTTG CAGTCTACGA GACGCTCAAG AATGCCTGGC TGCAGCACTA TGCAGTGAAC
AGCGCGGACC CCGGCGTGTT TGTGCTCCTG GCCTGTGGCA CCATGTCCAG TACCTGTGGC
CAGCTGGCCA GCTACCCCCT GGCCCTAGTC AGGACCCGGA TGCAGGCGCA AGCCTCTATT
GAGGGCGCTC CGGAGGTGAC CATGAGCAGC CTCTTCAAAC ATATCCTGCG GACCGAGGGG
GCCTTCGGGC TGTACAGGGG GCTGGCCCCC AACTTCATGA AGGTCATCCC AGCTGTGAGC
ATCAGCTACG TGGTCTACGA GAACCTGAAG ATCACCCTGG GCGTGCAGTC GCGGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006717012.1
CDS787..1923
Misc Feature(1)1069..1335(+)
Misc Feature(2)1339..1617(+)
Misc Feature(3)1633..1905(+)
Translation
Position Chain Variation Link
8 8 c, t dbSNP:772327879
9 9 a, g dbSNP:773391183
10 10 -, ga dbSNP:199536205
10 10 c, t dbSNP:761095361
11 11 a, g dbSNP:947623
18 18 c, t dbSNP:374996908
19 19 a, g dbSNP:759894728
20 20 g, t dbSNP:756771871
21 21 c, t dbSNP:763694616
26 26 a, g dbSNP:750989462
45 45 a, g dbSNP:541869510
98 98 c, t dbSNP:555810731
105 105 c, t dbSNP:575684321
114 114 a, g dbSNP:745604634
117 117 c, t dbSNP:561870813
123 123 c, t dbSNP:544709597
124 124 a, g dbSNP:564494501
134 134 c, t dbSNP:533576305
135 135 a, g dbSNP:769445260
149 149 c, g dbSNP:13298380
150 150 a, g dbSNP:560806745
177 177 a, t dbSNP:374379807
201 201 a, g dbSNP:528891555
244 244 c, g dbSNP:549025742
245 245 a, g dbSNP:749103673
255 255 c, t dbSNP:74627240
271 271 a, c dbSNP:368317938
340 340 g, t dbSNP:181978816
347 347 a, g dbSNP:531273159
361 361 c, t dbSNP:745442263
380 380 a, g dbSNP:551472775
392 392 a, g dbSNP:571305893
399 399 c, t dbSNP:774388751
420 420 a, g dbSNP:748156705
498 498 a, g dbSNP:116220772
507 507 a, g dbSNP:781035363
519 519 a, c dbSNP:547289264
521 521 c, t dbSNP:773300559
559 559 c, g dbSNP:73669633
571 571 c, t dbSNP:148201710
588 588 a, c dbSNP:555441827
620 620 c, t dbSNP:73612022
669 669 a, t dbSNP:771289854
671 671 a, g dbSNP:776822595
694 694 a, g dbSNP:760114660
698 698 a, g dbSNP:148566637
709 709 c, g dbSNP:138425637
721 721 a, c dbSNP:373725272
727 727 a, t dbSNP:761463295
728 728 a, g dbSNP:201454954
731 731 -, ggtgtttaa dbSNP:760359903
735 735 -, t dbSNP:34104513
758 758 a, g dbSNP:750100888
759 759 a, t dbSNP:755784724
766 766 c, t dbSNP:35473740
767 767 a, g dbSNP:758293495
769 769 a, g dbSNP:777854337
774 774 c, t dbSNP:747143850
775 775 a, g dbSNP:757331567
776 776 c, t dbSNP:370288653
777 777 a, g, t dbSNP:558972121
789 789 a, g dbSNP:770053771
798 798 a, g dbSNP:775882793
799 799 a, c, t dbSNP:771182815
800 800 a, g dbSNP:539274578
802 802 a, g dbSNP:760327859
803 803 a, t dbSNP:144383132
804 804 c, t dbSNP:776161764
821 821 a, c, t dbSNP:759162104
822 822 c, t dbSNP:752441598
840 840 a, t dbSNP:758170699
841 841 a, g dbSNP:746022262
848 848 a, g dbSNP:751498139
857 857 a, g dbSNP:776167122
860 860 c, t dbSNP:759135831
861 861 a, g dbSNP:571994044
867 867 a, t dbSNP:542169131
889 889 a, g dbSNP:755406713
890 890 c, t dbSNP:752496326
900 900 c, t dbSNP:748634777
901 901 a, g dbSNP:772583995
905 905 c, t dbSNP:748220703
906 906 a, g dbSNP:146596005
908 908 c, t dbSNP:574131158
911 911 c, t dbSNP:769292052
912 912 c, g dbSNP:142703551
915 915 c, t dbSNP:7859709
916 916 a, c, g, t dbSNP:369537472
924 924 c, t dbSNP:767528105
925 925 a, g dbSNP:750322632
954 954 c, t dbSNP:576891746
955 955 a, g dbSNP:151146132
964 964 a, g dbSNP:781364472
969 969 c, g, t dbSNP:754116133
970 970 a, g dbSNP:765719372
973 973 a, g dbSNP:753156452
975 975 c, t dbSNP:758660440
979 979 c, t dbSNP:778414541
998 998 c, t dbSNP:747459916
1019 1019 a, g dbSNP:752115914
1023 1023 a, g dbSNP:201631831
1025 1025 c, t dbSNP:370988341
1026 1026 a, g dbSNP:748832966
1029 1029 c, t dbSNP:754478157
1032 1032 a, g dbSNP:201869990
1050 1050 a, g dbSNP:747696280
1061 1061 c, t dbSNP:771904007
1062 1062 g, t dbSNP:772842561
1080 1080 c, g dbSNP:557639053
1081 1081 a, c dbSNP:771041847
1083 1083 g, t dbSNP:776680157
1107 1107 c, t dbSNP:199564503
1108 1108 a, g dbSNP:765220171
1109 1109 c, t dbSNP:140086444
1119 1119 c, t dbSNP:763022200
1124 1124 c, t dbSNP:372542969
1125 1125 a, g dbSNP:771071856
1127 1127 c, g dbSNP:751914863
1143 1143 c, t dbSNP:762130155
1148 1148 c, t dbSNP:768077378
1149 1149 a, g dbSNP:750914983
1171 1171 c, g, t dbSNP:150336698
1172 1172 a, g dbSNP:372118470
1176 1176 -, caa dbSNP:749342875
1179 1179 a, c dbSNP:781070275
1183 1183 a, g dbSNP:745836070
1184 1184 g, t dbSNP:766359255
1185 1185 a, g dbSNP:769573706
1190 1190 c, t dbSNP:753817818
1191 1191 c, t dbSNP:375402805
1192 1192 a, g dbSNP:780007071
1203 1203 c, t dbSNP:749392034
1204 1204 a, g dbSNP:755086874
1205 1205 c, t dbSNP:369203375
1207 1207 a, c dbSNP:748301336
1215 1215 c, t dbSNP:373056648
1217 1217 a, g dbSNP:144936572
1220 1220 a, g dbSNP:761079227
1224 1224 a, c dbSNP:766835860
1225 1225 a, g dbSNP:542635849
1231 1231 a, g dbSNP:762430803
1239 1239 c, g dbSNP:376606350
1243 1243 c, t dbSNP:370591194
1244 1244 a, g dbSNP:201242773
1250 1250 a, g dbSNP:767446116
1259 1259 a, g dbSNP:146433346
1261 1261 a, g dbSNP:750278943
1275 1275 a, c, t dbSNP:755929446
1279 1279 a, g dbSNP:749188523
1281 1281 a, g dbSNP:755015821
1287 1287 c, t dbSNP:778922590
1292 1292 a, g dbSNP:544724772
1296 1296 c, t dbSNP:772329874
1305 1305 c, t dbSNP:773391642
1319 1319 a, g dbSNP:770459495
1327 1327 g, t dbSNP:556095628
1339 1339 a, g dbSNP:371529907
1342 1342 a, t dbSNP:771644764
1350 1350 a, g dbSNP:772756499
1356 1356 c, t dbSNP:760327411
1357 1357 a, g dbSNP:575643847
1366 1366 a, g dbSNP:753708155
1369 1369 a, g dbSNP:759304684
1374 1374 g, t dbSNP:765217256
1375 1375 g, t dbSNP:752625486
1377 1377 c, g dbSNP:758475931
1392 1392 c, t dbSNP:777849436
1393 1393 a, g dbSNP:751749247
1401 1401 c, t dbSNP:757458201
1414 1414 a, g dbSNP:781236718
1432 1432 c, t dbSNP:142204445
1433 1433 a, g dbSNP:201422874
1435 1435 a, t dbSNP:779266773
1439 1439 c, t dbSNP:370055267
1440 1440 a, g dbSNP:372688826
1444 1444 c, t dbSNP:776350434
1445 1445 a, g dbSNP:145988246
1458 1458 c, g dbSNP:140777921
1461 1461 c, t dbSNP:775223622
1471 1471 c, t dbSNP:762703146
1477 1477 c, t dbSNP:764138101
1479 1479 c, t dbSNP:569190005
1488 1488 g, t dbSNP:761962113
1490 1490 c, t dbSNP:200130106
1497 1497 c, g dbSNP:767442589
1500 1500 a, g dbSNP:750699865
1503 1503 a, c, g dbSNP:531778526
1504 1504 c, g, t dbSNP:754304263
1507 1507 a, g dbSNP:779540699
1512 1512 c, t dbSNP:748536660
1515 1515 a, c, g dbSNP:142900894
1520 1520 a, g dbSNP:745548531
1527 1527 c, t dbSNP:769473901
1532 1532 c, t dbSNP:775097320
1533 1533 c, t dbSNP:377362242
1547 1547 c, g dbSNP:768273167
1548 1548 c, t dbSNP:774362337
1551 1551 -, c dbSNP:773599471
1551 1551 c, t dbSNP:144905207
1552 1552 a, g dbSNP:767669209
1554 1554 c, t dbSNP:773467573
1564 1564 a, g dbSNP:760816369
1565 1565 a, c, g dbSNP:367599861
1569 1569 c, t dbSNP:571794213
1590 1590 a, g, t dbSNP:527757590
1602 1602 c, t dbSNP:148311002
1608 1608 a, g dbSNP:369981036
1614 1614 c, g dbSNP:764639834
1617 1617 c, t dbSNP:752053223
1630 1630 a, g dbSNP:755638307
1632 1632 a, g dbSNP:141401867
1633 1633 a, g dbSNP:753561842
1638 1638 c, t dbSNP:754630387
1639 1639 a, g dbSNP:778606816
1642 1642 a, c, g dbSNP:747954096
1647 1647 c, t dbSNP:542867942
1650 1650 a, g dbSNP:746883992
1659 1659 c, g, t dbSNP:771156426
1662 1662 c, t dbSNP:759628591
1669 1669 a, g dbSNP:769955139
1675 1675 a, g, t dbSNP:775766266
1701 1701 -, c dbSNP:770577173
1704 1704 c, g dbSNP:764372216
1722 1722 c, t dbSNP:752085981
1724 1724 a, g dbSNP:762271253
1732 1732 a, g dbSNP:767983433
1733 1733 c, t dbSNP:753341608
1734 1734 a, g dbSNP:547576840
1746 1746 c, t dbSNP:369405997
1751 1751 a, g dbSNP:751448393
1752 1752 c, t dbSNP:150859583
1755 1755 c, t dbSNP:781056301
1757 1757 c, t dbSNP:373230180
1758 1758 a, g dbSNP:16930267
1765 1765 a, g dbSNP:780145419
1772 1772 a, g dbSNP:749338871
1773 1773 a, c dbSNP:768918046
1776 1776 c, t dbSNP:371151024
1790 1790 c, t dbSNP:201558106
1795 1795 c, t dbSNP:374458248
1796 1796 a, g dbSNP:772416779
1798 1798 a, g dbSNP:773606820
1808 1808 c, t dbSNP:142830945
1810 1810 c, t dbSNP:554406399
1812 1812 c, t dbSNP:775178382
1822 1822 a, g dbSNP:762527775
1837 1837 a, c dbSNP:763894403
1841 1841 a, t dbSNP:375698904
1843 1843 a, g dbSNP:761513312
1844 1844 c, t dbSNP:767280977
1854 1854 a, c dbSNP:750361886
1867 1867 a, g dbSNP:756121970
1874 1874 a, g dbSNP:779836096
1875 1875 c, t dbSNP:111440458
1876 1876 a, g dbSNP:755047936
1881 1881 c, t dbSNP:779114460
1884 1884 c, t dbSNP:748311616
1885 1885 a, g dbSNP:146124484
1897 1897 a, g dbSNP:772346771
1899 1899 c, t dbSNP:778158911
1902 1902 c, t dbSNP:747304394
1903 1903 c, g dbSNP:771515643
1906 1906 a, g dbSNP:777263284
1908 1908 c, t dbSNP:762615145
1909 1909 a, g dbSNP:370283153
1917 1917 a, g dbSNP:774155897
1919 1919 a, g dbSNP:199739089
1924 1924 -, g dbSNP:759027068
1924 1924 c, t dbSNP:369962505
1925 1925 a, g, t dbSNP:201844320
1925 1925 -, g dbSNP:765268245
1927 1927 a, c, g, t dbSNP:200194334
1930 1930 a, g, t dbSNP:201248373
1931 1931 a, c, g dbSNP:577175137
1936 1936 c, t dbSNP:752894376
1937 1937 a, g dbSNP:377049698
1940 1940 a, c, t dbSNP:370271137
1941 1941 a, g dbSNP:374588749
1953 1953 a, g dbSNP:781764705
1954 1954 c, g dbSNP:746317417
1966 1966 c, t dbSNP:768300868
1967 1967 a, g dbSNP:199878817
1971 1971 a, c, g, t dbSNP:775510321
1972 1972 c, t dbSNP:760523646
1973 1973 c, t dbSNP:141910331
1996 1996 g, t dbSNP:547868115
2023 2023 c, t dbSNP:561255124
2032 2032 c, t dbSNP:530300344
2050 2050 c, t dbSNP:745933780
2054 2054 c, t dbSNP:770024989
2055 2055 a, g dbSNP:75511005
2077 2077 a, g dbSNP:186440107
2079 2079 a, c dbSNP:760534129
2095 2095 a, c dbSNP:532623293
2098 2098 c, t dbSNP:13298797
2100 2100 a, g dbSNP:552462679
2119 2119 a, g dbSNP:565329443
2130 2130 c, g, t dbSNP:114790720
2177 2177 c, t dbSNP:541860686
2183 2183 c, t dbSNP:780375217
2194 2194 a, g dbSNP:372694601
2223 2223 c, t dbSNP:7872702
2224 2224 a, g dbSNP:140738643
2242 2242 c, t dbSNP:577113755
2262 2262 c, t dbSNP:374091374
2287 2287 c, t dbSNP:10987876
2288 2288 a, c, g dbSNP:188541776
2309 2309 c, t dbSNP:369881030
2310 2310 a, g dbSNP:111828101
2313 2313 c, g dbSNP:572383902
2333 2333 -, ctgc dbSNP:567835255
2340 2340 a, c dbSNP:750834588
2352 2352 a, g dbSNP:541101417
2379 2379 a, c dbSNP:376171587
2418 2418 a, c dbSNP:369199483
2436 2436 c, g dbSNP:561350806
2458 2458 c, g dbSNP:3814524
2471 2471 a, g dbSNP:760444702
2522 2522 c, t dbSNP:767020937
2535 2535 c, t dbSNP:543515646
2579 2579 c, t dbSNP:564699238
2606 2606 -, g dbSNP:34224940
2656 2656 a, g dbSNP:770879666
2674 2674 a, c dbSNP:757971886
2684 2684 c, t dbSNP:563501308
2691 2691 a, g dbSNP:532609821
2703 2703 a, g dbSNP:552446244
2729 2729 c, t dbSNP:566269410
2730 2730 a, g dbSNP:746741415
2735 2735 a, g dbSNP:756864174
2738 2738 c, g dbSNP:528071747
2741 2741 a, c dbSNP:116513398
2750 2750 a, g dbSNP:180947406
2780 2780 a, g dbSNP:537095783
2781 2781 a, g dbSNP:185116755
2784 2784 g, t dbSNP:745683615
2829 2829 c, t dbSNP:57052978
2831 2831 a, g dbSNP:769689731
2867 2867 c, t dbSNP:147539248
2868 2868 -, gag dbSNP:567976888
2870 2870 a, g dbSNP:775457386
2910 2910 a, c, t dbSNP:749315548
2938 2938 c, t dbSNP:139384794
2945 2945 a, g dbSNP:536517546
3022 3022 a, t dbSNP:774303940
3025 3025 -, ctaa dbSNP:528213954
3026 3026 -, ctaa dbSNP:777101904
3029 3029 -, ctaa dbSNP:773891780
3041 3041 c, g dbSNP:542129619
3076 3076 c, t dbSNP:191060669
3167 3167 c, t dbSNP:764745985
3191 3191 c, g dbSNP:767657703
3222 3222 c, t dbSNP:773668033
3223 3223 a, g dbSNP:574788142
3248 3248 -, t dbSNP:35604217
3301 3301 a, t dbSNP:537978043
3313 3313 c, t dbSNP:761158288
3314 3314 a, g dbSNP:766965728
3345 3345 g, t dbSNP:374692425
3371 3371 c, t dbSNP:532543390
3397 3397 c, t dbSNP:546018894
3411 3411 a, t dbSNP:559910298
3440 3440 c, t dbSNP:528751634
3451 3451 a, g dbSNP:757840839
3472 3472 c, g dbSNP:369941858
3475 3475 a, c, g dbSNP:10760538
3477 3477 -, g dbSNP:35358699
3614 3614 c, t dbSNP:561483406
3643 3643 a, g dbSNP:35037027
3652 3652 a, c dbSNP:751043182
3672 3672 c, t dbSNP:530560615
3680 3680 a, g dbSNP:184011703
3718 3718 a, g dbSNP:77244294

Target ORF information:

RefSeq Version XM_006716949
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006716949

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGTCCC TGCGGGACTT GGGAGTCAAG ATATCTGAAC AGCAGGCAGA AAAAATTCTC 
AAGAGAATAC GAACGGGCCA TTTCTGGGGC CCTGTCACCT ACATGGATAA AAACGGCACG
ATGACCATTG ACTGGAACGA GTGGAGAGAC TACCACCTCC TCCACCCCGT GGAAAACATC
CCCGAGATCA TCCTCTACTG GAAGCATTCC ACGATCTTTG ATGTGGGTGA GAATCTAACG
GTCCCGGATG AGTTCACAGT GGAGGAGAGG CAGACGGGGA TGTGGTGGAG ACACCTGGTG
GCAGGAGGTG GGGCAGGGGC CGTATCCAGA ACCTGCACGG CCCCCCTGGA CAGGCTCAAG
GTGCTCATGC AGGTCCATGC CTCCCGCAGC AACAACATGG GCATCGTTGG TGGCTTCACT
CAGATGATTC GAGAAGGAGG GGCCAGGTCA CTCTGGCGGG GCAATGGCAT CAACGTCCTC
AAAATTGCCC CCGAATCAGC CATCAAATTC ATGGCCTATG AGCAGATCAA GCGCCTTGTT
GGTAGTGACC AGGAGACTCT GAGGATTCAC GAGAGGCTTG TGGCAGGGTC CTTGGCAGGG
GCCATCGCCC AGAGCAGCAT CTACCCAATG GAGGTCCTGA AGACCCGGAT GGCGCTGCGG
AAGACAGGCC AGTACTCAGG AATGCTGGAC TGCGCCAGGA GGATCCTGGC CAGAGAGGGG
GTGGCCGCCT TCTACAAAGG CTATGTCCCC AACATGCTGG GCATCATCCC CTATGCCGGC
ATCGACCTTG CAGTCTACGA GACGCTCAAG AATGCCTGGC TGCAGCACTA TGCAGTGAAC
AGCGCGGACC CCGGCGTGTT TGTGCTCCTG GCCTGTGGCA CCATGTCCAG TACCTGTGGC
CAGCTGGCCA GCTACCCCCT GGCCCTAGTC AGGACCCGGA TGCAGGCGCA AGCCTCTATT
GAGGGCGCTC CGGAGGTGAC CATGAGCAGC CTCTTCAAAC ATATCCTGCG GACCGAGGGG
GCCTTCGGGC TGTACAGGGG GCTGGCCCCC AACTTCATGA AGGTCATCCC AGCTGTGAGC
ATCAGCTACG TGGTCTACGA GAACCTGAAG ATCACCCTGG GCGTGCAGTC GCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu42966
Accession Version XM_005251689.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1101bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product calcium-binding mitochondrial carrier protein SCaMC-2 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008470.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578817038. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGTCCC TGCGGGACTT GGGAGTCAAG ATATCTGAAC AGCAGGCAGA AAAAATTCTC 
AAGAGCATGG ATAAAAACGG CACGATGACC ATTGACTGGA ACGAGTGGAG AGACTACCAC
CTCCTCCACC CCGTGGAAAA CATCCCCGAG ATCATCCTCT ACTGGAAGCA TTCCACGATC
TTTGATGTGG GTGAGAATCT AACGGTCCCG GATGAGTTCA CAGTGGAGGA GAGGCAGACG
GGGATGTGGT GGAGACACCT GGTGGCAGGA GGTGGGGCAG GGGCCGTATC CAGAACCTGC
ACGGCCCCCC TGGACAGGCT CAAGGTGCTC ATGCAGGTCC ATGCCTCCCG CAGCAACAAC
ATGGGCATCG TTGGTGGCTT CACTCAGATG ATTCGAGAAG GAGGGGCCAG GTCACTCTGG
CGGGGCAATG GCATCAACGT CCTCAAAATT GCCCCCGAAT CAGCCATCAA ATTCATGGCC
TATGAGCAGA TCAAGCGCCT TGTTGGTAGT GACCAGGAGA CTCTGAGGAT TCACGAGAGG
CTTGTGGCAG GGTCCTTGGC AGGGGCCATC GCCCAGAGCA GCATCTACCC AATGGAGGTC
CTGAAGACCC GGATGGCGCT GCGGAAGACA GGCCAGTACT CAGGAATGCT GGACTGCGCC
AGGAGGATCC TGGCCAGAGA GGGGGTGGCC GCCTTCTACA AAGGCTATGT CCCCAACATG
CTGGGCATCA TCCCCTATGC CGGCATCGAC CTTGCAGTCT ACGAGACGCT CAAGAATGCC
TGGCTGCAGC ACTATGCAGT GAACAGCGCG GACCCCGGCG TGTTTGTGCT CCTGGCCTGT
GGCACCATGT CCAGTACCTG TGGCCAGCTG GCCAGCTACC CCCTGGCCCT AGTCAGGACC
CGGATGCAGG CGCAAGCCTC TATTGAGGGC GCTCCGGAGG TGACCATGAG CAGCCTCTTC
AAACATATCC TGCGGACCGA GGGGGCCTTC GGGCTGTACA GGGGGCTGGC CCCCAACTTC
ATGAAGGTCA TCCCAGCTGT GAGCATCAGC TACGTGGTCT ACGAGAACCT GAAGATCACC
CTGGGCGTGC AGTCGCGGTG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005251746.1
CDS787..1887
Misc Feature(1)796..900(+)
Misc Feature(2)856..891(+)
Misc Feature(3)1033..1299(+)
Misc Feature(4)1303..1581(+)
Misc Feature(5)1597..1869(+)
Translation
Position Chain Variation Link
8 8 c, t dbSNP:772327879
9 9 a, g dbSNP:773391183
10 10 -, ga dbSNP:199536205
10 10 c, t dbSNP:761095361
11 11 a, g dbSNP:947623
18 18 c, t dbSNP:374996908
19 19 a, g dbSNP:759894728
20 20 g, t dbSNP:756771871
21 21 c, t dbSNP:763694616
26 26 a, g dbSNP:750989462
45 45 a, g dbSNP:541869510
98 98 c, t dbSNP:555810731
105 105 c, t dbSNP:575684321
114 114 a, g dbSNP:745604634
117 117 c, t dbSNP:561870813
123 123 c, t dbSNP:544709597
124 124 a, g dbSNP:564494501
134 134 c, t dbSNP:533576305
135 135 a, g dbSNP:769445260
149 149 c, g dbSNP:13298380
150 150 a, g dbSNP:560806745
177 177 a, t dbSNP:374379807
201 201 a, g dbSNP:528891555
244 244 c, g dbSNP:549025742
245 245 a, g dbSNP:749103673
255 255 c, t dbSNP:74627240
271 271 a, c dbSNP:368317938
340 340 g, t dbSNP:181978816
347 347 a, g dbSNP:531273159
361 361 c, t dbSNP:745442263
380 380 a, g dbSNP:551472775
392 392 a, g dbSNP:571305893
399 399 c, t dbSNP:774388751
420 420 a, g dbSNP:748156705
498 498 a, g dbSNP:116220772
507 507 a, g dbSNP:781035363
519 519 a, c dbSNP:547289264
521 521 c, t dbSNP:773300559
559 559 c, g dbSNP:73669633
571 571 c, t dbSNP:148201710
588 588 a, c dbSNP:555441827
620 620 c, t dbSNP:73612022
669 669 a, t dbSNP:771289854
671 671 a, g dbSNP:776822595
694 694 a, g dbSNP:760114660
698 698 a, g dbSNP:148566637
709 709 c, g dbSNP:138425637
721 721 a, c dbSNP:373725272
727 727 a, t dbSNP:761463295
728 728 a, g dbSNP:201454954
731 731 -, ggtgtttaa dbSNP:760359903
735 735 -, t dbSNP:34104513
758 758 a, g dbSNP:750100888
759 759 a, t dbSNP:755784724
766 766 c, t dbSNP:35473740
767 767 a, g dbSNP:758293495
769 769 a, g dbSNP:777854337
774 774 c, t dbSNP:747143850
775 775 a, g dbSNP:757331567
776 776 c, t dbSNP:370288653
777 777 a, g, t dbSNP:558972121
789 789 a, g dbSNP:770053771
798 798 a, g dbSNP:775882793
799 799 a, c, t dbSNP:771182815
800 800 a, g dbSNP:539274578
802 802 a, g dbSNP:760327859
803 803 a, t dbSNP:144383132
804 804 c, t dbSNP:776161764
821 821 a, c, t dbSNP:759162104
822 822 c, t dbSNP:752441598
840 840 a, t dbSNP:758170699
841 841 a, g dbSNP:746022262
848 848 a, g dbSNP:751498139
853 853 a, g dbSNP:755406713
854 854 c, t dbSNP:752496326
864 864 c, t dbSNP:748634777
865 865 a, g dbSNP:772583995
869 869 c, t dbSNP:748220703
870 870 a, g dbSNP:146596005
872 872 c, t dbSNP:574131158
875 875 c, t dbSNP:769292052
876 876 c, g dbSNP:142703551
879 879 c, t dbSNP:7859709
880 880 a, c, g, t dbSNP:369537472
888 888 c, t dbSNP:767528105
889 889 a, g dbSNP:750322632
918 918 c, t dbSNP:576891746
919 919 a, g dbSNP:151146132
928 928 a, g dbSNP:781364472
933 933 c, g, t dbSNP:754116133
934 934 a, g dbSNP:765719372
937 937 a, g dbSNP:753156452
939 939 c, t dbSNP:758660440
943 943 c, t dbSNP:778414541
962 962 c, t dbSNP:747459916
983 983 a, g dbSNP:752115914
987 987 a, g dbSNP:201631831
989 989 c, t dbSNP:370988341
990 990 a, g dbSNP:748832966
993 993 c, t dbSNP:754478157
996 996 a, g dbSNP:201869990
1014 1014 a, g dbSNP:747696280
1025 1025 c, t dbSNP:771904007
1026 1026 g, t dbSNP:772842561
1044 1044 c, g dbSNP:557639053
1045 1045 a, c dbSNP:771041847
1047 1047 g, t dbSNP:776680157
1071 1071 c, t dbSNP:199564503
1072 1072 a, g dbSNP:765220171
1073 1073 c, t dbSNP:140086444
1083 1083 c, t dbSNP:763022200
1088 1088 c, t dbSNP:372542969
1089 1089 a, g dbSNP:771071856
1091 1091 c, g dbSNP:751914863
1107 1107 c, t dbSNP:762130155
1112 1112 c, t dbSNP:768077378
1113 1113 a, g dbSNP:750914983
1135 1135 c, g, t dbSNP:150336698
1136 1136 a, g dbSNP:372118470
1140 1140 -, caa dbSNP:749342875
1143 1143 a, c dbSNP:781070275
1147 1147 a, g dbSNP:745836070
1148 1148 g, t dbSNP:766359255
1149 1149 a, g dbSNP:769573706
1154 1154 c, t dbSNP:753817818
1155 1155 c, t dbSNP:375402805
1156 1156 a, g dbSNP:780007071
1167 1167 c, t dbSNP:749392034
1168 1168 a, g dbSNP:755086874
1169 1169 c, t dbSNP:369203375
1171 1171 a, c dbSNP:748301336
1179 1179 c, t dbSNP:373056648
1181 1181 a, g dbSNP:144936572
1184 1184 a, g dbSNP:761079227
1188 1188 a, c dbSNP:766835860
1189 1189 a, g dbSNP:542635849
1195 1195 a, g dbSNP:762430803
1203 1203 c, g dbSNP:376606350
1207 1207 c, t dbSNP:370591194
1208 1208 a, g dbSNP:201242773
1214 1214 a, g dbSNP:767446116
1223 1223 a, g dbSNP:146433346
1225 1225 a, g dbSNP:750278943
1239 1239 a, c, t dbSNP:755929446
1243 1243 a, g dbSNP:749188523
1245 1245 a, g dbSNP:755015821
1251 1251 c, t dbSNP:778922590
1256 1256 a, g dbSNP:544724772
1260 1260 c, t dbSNP:772329874
1269 1269 c, t dbSNP:773391642
1283 1283 a, g dbSNP:770459495
1291 1291 g, t dbSNP:556095628
1303 1303 a, g dbSNP:371529907
1306 1306 a, t dbSNP:771644764
1314 1314 a, g dbSNP:772756499
1320 1320 c, t dbSNP:760327411
1321 1321 a, g dbSNP:575643847
1330 1330 a, g dbSNP:753708155
1333 1333 a, g dbSNP:759304684
1338 1338 g, t dbSNP:765217256
1339 1339 g, t dbSNP:752625486
1341 1341 c, g dbSNP:758475931
1356 1356 c, t dbSNP:777849436
1357 1357 a, g dbSNP:751749247
1365 1365 c, t dbSNP:757458201
1378 1378 a, g dbSNP:781236718
1396 1396 c, t dbSNP:142204445
1397 1397 a, g dbSNP:201422874
1399 1399 a, t dbSNP:779266773
1403 1403 c, t dbSNP:370055267
1404 1404 a, g dbSNP:372688826
1408 1408 c, t dbSNP:776350434
1409 1409 a, g dbSNP:145988246
1422 1422 c, g dbSNP:140777921
1425 1425 c, t dbSNP:775223622
1435 1435 c, t dbSNP:762703146
1441 1441 c, t dbSNP:764138101
1443 1443 c, t dbSNP:569190005
1452 1452 g, t dbSNP:761962113
1454 1454 c, t dbSNP:200130106
1461 1461 c, g dbSNP:767442589
1464 1464 a, g dbSNP:750699865
1467 1467 a, c, g dbSNP:531778526
1468 1468 c, g, t dbSNP:754304263
1471 1471 a, g dbSNP:779540699
1476 1476 c, t dbSNP:748536660
1479 1479 a, c, g dbSNP:142900894
1484 1484 a, g dbSNP:745548531
1491 1491 c, t dbSNP:769473901
1496 1496 c, t dbSNP:775097320
1497 1497 c, t dbSNP:377362242
1511 1511 c, g dbSNP:768273167
1512 1512 c, t dbSNP:774362337
1515 1515 -, c dbSNP:773599471
1515 1515 c, t dbSNP:144905207
1516 1516 a, g dbSNP:767669209
1518 1518 c, t dbSNP:773467573
1528 1528 a, g dbSNP:760816369
1529 1529 a, c, g dbSNP:367599861
1533 1533 c, t dbSNP:571794213
1554 1554 a, g, t dbSNP:527757590
1566 1566 c, t dbSNP:148311002
1572 1572 a, g dbSNP:369981036
1578 1578 c, g dbSNP:764639834
1581 1581 c, t dbSNP:752053223
1594 1594 a, g dbSNP:755638307
1596 1596 a, g dbSNP:141401867
1597 1597 a, g dbSNP:753561842
1602 1602 c, t dbSNP:754630387
1603 1603 a, g dbSNP:778606816
1606 1606 a, c, g dbSNP:747954096
1611 1611 c, t dbSNP:542867942
1614 1614 a, g dbSNP:746883992
1623 1623 c, g, t dbSNP:771156426
1626 1626 c, t dbSNP:759628591
1633 1633 a, g dbSNP:769955139
1639 1639 a, g, t dbSNP:775766266
1665 1665 -, c dbSNP:770577173
1668 1668 c, g dbSNP:764372216
1686 1686 c, t dbSNP:752085981
1688 1688 a, g dbSNP:762271253
1696 1696 a, g dbSNP:767983433
1697 1697 c, t dbSNP:753341608
1698 1698 a, g dbSNP:547576840
1710 1710 c, t dbSNP:369405997
1715 1715 a, g dbSNP:751448393
1716 1716 c, t dbSNP:150859583
1719 1719 c, t dbSNP:781056301
1721 1721 c, t dbSNP:373230180
1722 1722 a, g dbSNP:16930267
1729 1729 a, g dbSNP:780145419
1736 1736 a, g dbSNP:749338871
1737 1737 a, c dbSNP:768918046
1740 1740 c, t dbSNP:371151024
1754 1754 c, t dbSNP:201558106
1759 1759 c, t dbSNP:374458248
1760 1760 a, g dbSNP:772416779
1762 1762 a, g dbSNP:773606820
1772 1772 c, t dbSNP:142830945
1774 1774 c, t dbSNP:554406399
1776 1776 c, t dbSNP:775178382
1786 1786 a, g dbSNP:762527775
1801 1801 a, c dbSNP:763894403
1805 1805 a, t dbSNP:375698904
1807 1807 a, g dbSNP:761513312
1808 1808 c, t dbSNP:767280977
1818 1818 a, c dbSNP:750361886
1831 1831 a, g dbSNP:756121970
1838 1838 a, g dbSNP:779836096
1839 1839 c, t dbSNP:111440458
1840 1840 a, g dbSNP:755047936
1845 1845 c, t dbSNP:779114460
1848 1848 c, t dbSNP:748311616
1849 1849 a, g dbSNP:146124484
1861 1861 a, g dbSNP:772346771
1863 1863 c, t dbSNP:778158911
1866 1866 c, t dbSNP:747304394
1867 1867 c, g dbSNP:771515643
1870 1870 a, g dbSNP:777263284
1872 1872 c, t dbSNP:762615145
1873 1873 a, g dbSNP:370283153
1881 1881 a, g dbSNP:774155897
1883 1883 a, g dbSNP:199739089
1888 1888 -, g dbSNP:759027068
1888 1888 c, t dbSNP:369962505
1889 1889 a, g, t dbSNP:201844320
1889 1889 -, g dbSNP:765268245
1891 1891 a, c, g, t dbSNP:200194334
1894 1894 a, g, t dbSNP:201248373
1895 1895 a, c, g dbSNP:577175137
1900 1900 c, t dbSNP:752894376
1901 1901 a, g dbSNP:377049698
1904 1904 a, c, t dbSNP:370271137
1905 1905 a, g dbSNP:374588749
1917 1917 a, g dbSNP:781764705
1918 1918 c, g dbSNP:746317417
1930 1930 c, t dbSNP:768300868
1931 1931 a, g dbSNP:199878817
1935 1935 a, c, g, t dbSNP:775510321
1936 1936 c, t dbSNP:760523646
1937 1937 c, t dbSNP:141910331
1960 1960 g, t dbSNP:547868115
1987 1987 c, t dbSNP:561255124
1996 1996 c, t dbSNP:530300344
2014 2014 c, t dbSNP:745933780
2018 2018 c, t dbSNP:770024989
2019 2019 a, g dbSNP:75511005
2041 2041 a, g dbSNP:186440107
2043 2043 a, c dbSNP:760534129
2059 2059 a, c dbSNP:532623293
2062 2062 c, t dbSNP:13298797
2064 2064 a, g dbSNP:552462679
2083 2083 a, g dbSNP:565329443
2094 2094 c, g, t dbSNP:114790720
2141 2141 c, t dbSNP:541860686
2147 2147 c, t dbSNP:780375217
2158 2158 a, g dbSNP:372694601
2187 2187 c, t dbSNP:7872702
2188 2188 a, g dbSNP:140738643
2206 2206 c, t dbSNP:577113755
2226 2226 c, t dbSNP:374091374
2251 2251 c, t dbSNP:10987876
2252 2252 a, c, g dbSNP:188541776
2273 2273 c, t dbSNP:369881030
2274 2274 a, g dbSNP:111828101
2277 2277 c, g dbSNP:572383902
2297 2297 -, ctgc dbSNP:567835255
2304 2304 a, c dbSNP:750834588
2316 2316 a, g dbSNP:541101417
2343 2343 a, c dbSNP:376171587
2382 2382 a, c dbSNP:369199483
2400 2400 c, g dbSNP:561350806
2422 2422 c, g dbSNP:3814524
2435 2435 a, g dbSNP:760444702
2486 2486 c, t dbSNP:767020937
2499 2499 c, t dbSNP:543515646
2543 2543 c, t dbSNP:564699238
2570 2570 -, g dbSNP:34224940
2620 2620 a, g dbSNP:770879666
2638 2638 a, c dbSNP:757971886
2648 2648 c, t dbSNP:563501308
2655 2655 a, g dbSNP:532609821
2667 2667 a, g dbSNP:552446244
2693 2693 c, t dbSNP:566269410
2694 2694 a, g dbSNP:746741415
2699 2699 a, g dbSNP:756864174
2702 2702 c, g dbSNP:528071747
2705 2705 a, c dbSNP:116513398
2714 2714 a, g dbSNP:180947406
2744 2744 a, g dbSNP:537095783
2745 2745 a, g dbSNP:185116755
2748 2748 g, t dbSNP:745683615
2793 2793 c, t dbSNP:57052978
2795 2795 a, g dbSNP:769689731
2831 2831 c, t dbSNP:147539248
2832 2832 -, gag dbSNP:567976888
2834 2834 a, g dbSNP:775457386
2874 2874 a, c, t dbSNP:749315548
2902 2902 c, t dbSNP:139384794
2909 2909 a, g dbSNP:536517546
2986 2986 a, t dbSNP:774303940
2989 2989 -, ctaa dbSNP:528213954
2990 2990 -, ctaa dbSNP:777101904
2993 2993 -, ctaa dbSNP:773891780
3005 3005 c, g dbSNP:542129619
3040 3040 c, t dbSNP:191060669
3131 3131 c, t dbSNP:764745985
3155 3155 c, g dbSNP:767657703
3186 3186 c, t dbSNP:773668033
3187 3187 a, g dbSNP:574788142
3212 3212 -, t dbSNP:35604217
3265 3265 a, t dbSNP:537978043
3277 3277 c, t dbSNP:761158288
3278 3278 a, g dbSNP:766965728
3309 3309 g, t dbSNP:374692425
3335 3335 c, t dbSNP:532543390
3361 3361 c, t dbSNP:546018894
3375 3375 a, t dbSNP:559910298
3404 3404 c, t dbSNP:528751634
3415 3415 a, g dbSNP:757840839
3436 3436 c, g dbSNP:369941858
3439 3439 a, c, g dbSNP:10760538
3441 3441 -, g dbSNP:35358699
3578 3578 c, t dbSNP:561483406
3607 3607 a, g dbSNP:35037027
3616 3616 a, c dbSNP:751043182
3636 3636 c, t dbSNP:530560615
3644 3644 a, g dbSNP:184011703
3682 3682 a, g dbSNP:77244294

Target ORF information:

RefSeq Version XM_005251689
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005251689

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCAGTCCC TGCGGGACTT GGGAGTCAAG ATATCTGAAC AGCAGGCAGA AAAAATTCTC 
AAGAGCATGG ATAAAAACGG CACGATGACC ATTGACTGGA ACGAGTGGAG AGACTACCAC
CTCCTCCACC CCGTGGAAAA CATCCCCGAG ATCATCCTCT ACTGGAAGCA TTCCACGATC
TTTGATGTGG GTGAGAATCT AACGGTCCCG GATGAGTTCA CAGTGGAGGA GAGGCAGACG
GGGATGTGGT GGAGACACCT GGTGGCAGGA GGTGGGGCAG GGGCCGTATC CAGAACCTGC
ACGGCCCCCC TGGACAGGCT CAAGGTGCTC ATGCAGGTCC ATGCCTCCCG CAGCAACAAC
ATGGGCATCG TTGGTGGCTT CACTCAGATG ATTCGAGAAG GAGGGGCCAG GTCACTCTGG
CGGGGCAATG GCATCAACGT CCTCAAAATT GCCCCCGAAT CAGCCATCAA ATTCATGGCC
TATGAGCAGA TCAAGCGCCT TGTTGGTAGT GACCAGGAGA CTCTGAGGAT TCACGAGAGG
CTTGTGGCAG GGTCCTTGGC AGGGGCCATC GCCCAGAGCA GCATCTACCC AATGGAGGTC
CTGAAGACCC GGATGGCGCT GCGGAAGACA GGCCAGTACT CAGGAATGCT GGACTGCGCC
AGGAGGATCC TGGCCAGAGA GGGGGTGGCC GCCTTCTACA AAGGCTATGT CCCCAACATG
CTGGGCATCA TCCCCTATGC CGGCATCGAC CTTGCAGTCT ACGAGACGCT CAAGAATGCC
TGGCTGCAGC ACTATGCAGT GAACAGCGCG GACCCCGGCG TGTTTGTGCT CCTGGCCTGT
GGCACCATGT CCAGTACCTG TGGCCAGCTG GCCAGCTACC CCCTGGCCCT AGTCAGGACC
CGGATGCAGG CGCAAGCCTC TATTGAGGGC GCTCCGGAGG TGACCATGAG CAGCCTCTTC
AAACATATCC TGCGGACCGA GGGGGCCTTC GGGCTGTACA GGGGGCTGGC CCCCAACTTC
ATGAAGGTCA TCCCAGCTGT GAGCATCAGC TACGTGGTCT ACGAGAACCT GAAGATCACC
CTGGGCGTGC AGTCGCGGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu12516
Accession Version NM_001006642.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product calcium-binding mitochondrial carrier protein SCaMC-2 isoform c precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB067483.1, DA219774.1, AL360268.14, AJ619991.1 and BM689843.1. On Jul 11, 2012 this sequence version replaced gi:388453817. Summary: The protein encoded by this gene belongs to the family of calcium-binding mitochondrial carriers, with a characteristic mitochondrial carrier domain at the C-terminus. These proteins are found in the inner membranes of mitochondria, and function as transport proteins. They shuttle metabolites, nucleotides and cofactors through the mitochondrial membrane and thereby connect and/or regulate cytoplasm and matrix functions. This protein may function as an ATP-Mg/Pi carrier that mediates the transport of Mg-ATP in exchange for phosphate, and likely responsible for the net uptake or efflux of adenine nucleotides into or from the mitochondria. Alternatively spliced transcript variants encoding different isoforms with a common C-terminus but variable N-termini have been described for this gene. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (3) contains an alternate 5' terminal exon compared to variant 1. It initiates translation from an upstream AUG and encodes a longer isoform (c) with a distinct N-terminus compared to isoform a. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AJ619963.1, AJ619991.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGCAGA TGCTGTGGCA TTTCCTAGCT AGCTTTTTCC CCAGGGCTGG GTGCCACGGC 
TCCAGAGAGG GGGACGATCG TGAAGTCAGA GGCACCCCAG CCCCTGCCTG GAGAGACCAG
ATGGCAAGCT TTTTGGGGAA ACAGGACGGA AGGGCTGAGG CCACGGAAAA AAGACCCACC
ATTTTGCTGG TGGTTGGACC TGCAGAGCAG TTTCCTAAGA AAATTGTACA AGCTGGAGAT
AAGGACCTTG ATGGGCAGCT AGACTTTGAA GAATTTGTCC ATTATCTCCA AGATCATGAG
AAGAAGCTGA GGCTGGTGTT TAAGAGTTTG GACAAAAAGA ATGATGGACG CATTGACGCG
CAGGAGATCA TGCAGTCCCT GCGGGACTTG GGAGTCAAGA TATCTGAACA GCAGGCAGAA
AAAATTCTCA AGAGCATGGA TAAAAACGGC ACGATGACCA TCGACTGGAA CGAGTGGAGA
GACTACCACC TCCTCCACCC CGTGGAAAAC ATCCCCGAGA TCATCCTCTA CTGGAAGCAT
TCCACGATCT TTGATGTGGG TGAGAATCTA ACGGTCCCGG ATGAGTTCAC AGTGGAGGAG
AGGCAGACGG GGATGTGGTG GAGACACCTG GTGGCAGGAG GTGGGGCAGG GGCCGTATCC
AGAACCTGCA CGGCCCCCCT GGACAGGCTC AAGGTGCTCA TGCAGGTCCA TGCCTCCCGC
AGCAACAACA TGGGCATCGT TGGTGGCTTC ACTCAGATGA TTCGAGAAGG AGGGGCCAGG
TCACTCTGGC GGGGCAATGG CATCAACGTC CTCAAAATTG CCCCCGAATC AGCCATCAAA
TTCATGGCCT ATGAGCAGAT CAAGCGCCTT GTTGGTAGTG ACCAGGAGAC TCTGAGGATT
CACGAGAGGC TTGTGGCAGG GTCCTTGGCA GGGGCCATCG CCCAGAGCAG CATCTACCCA
ATGGAGGTCC TGAAGACCCG GATGGCGCTG CGGAAGACAG GCCAGTACTC AGGAATGCTG
GACTGCGCCA GGAGGATCCT GGCCAGAGAG GGGGTGGCCG CCTTCTACAA AGGCTATGTC
CCCAACATGC TGGGCATCAT CCCCTATGCC GGCATCGACC TTGCAGTCTA CGAGACGCTC
AAGAATGCCT GGCTGCAGCA CTATGCAGTG AACAGCGCGG ACCCCGGCGT GTTTGTGCTC
CTGGCCTGTG GCACCATGTC CAGTACCTGT GGCCAGCTGG CCAGCTACCC CCTGGCCCTA
GTCAGGACCC GGATGCAGGC GCAAGCCTCT ATTGAGGGCG CTCCGGAGGT GACCATGAGC
AGCCTCTTCA AACATATCCT GCGGACCGAG GGGGCCTTCG GGCTGTACAG GGGGCTGGCC
CCCAACTTCA TGAAGGTCAT CCCAGCTGTG AGCATCAGCT ACGTGGTCTA CGAGAACCTG
AAGATCACCC TGGGCGTGCA GTCGCGGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001006643.1
CDS408..1877
Misc Feature(1)204..206(+)
Misc Feature(2)594..698(+)
Misc Feature(3)645..782(+)
Misc Feature(4)645..680(+)
Misc Feature(5)711..890(+)
Misc Feature(6)729..893(+)
Misc Feature(7)738..881(+)
Misc Feature(8)1023..1289(+)
Misc Feature(9)1293..1571(+)
Misc Feature(10)1587..1859(+)
Exon (1)1..626
Gene:SLC25A25
Gene Synonym:
Exon (2)627..753
Gene:SLC25A25
Gene Synonym:
Exon (3)754..841
Gene:SLC25A25
Gene Synonym:
Exon (4)842..953
Gene:SLC25A25
Gene Synonym:
Exon (5)954..1112
Gene:SLC25A25
Gene Synonym:
Exon (6)1113..1265
Gene:SLC25A25
Gene Synonym:
Exon (7)1266..1373
Gene:SLC25A25
Gene Synonym:
Exon (8)1374..1541
Gene:SLC25A25
Gene Synonym:
Exon (9)1542..1692
Gene:SLC25A25
Gene Synonym:
Exon (10)1693..3691
Gene:SLC25A25
Gene Synonym:
Translation
Position Chain Variation Link
3 3 a, g dbSNP:763206211
5 5 a, g dbSNP:372408879
84 84 -, tttt dbSNP:757342831
95 95 aaaa, tttt dbSNP:71495680
95 95 a, t dbSNP:36184462
96 96 -, a dbSNP:773958605
96 96 a, t dbSNP:118130288
97 97 a, t dbSNP:116192359
98 98 -, aaa dbSNP:367933740
98 98 a, t dbSNP:115127124
106 106 -, aa dbSNP:59794334
107 107 a, g dbSNP:552582228
114 114 a, g dbSNP:562172327
125 125 a, g dbSNP:566026559
167 167 a, c dbSNP:528616553
172 172 c, t dbSNP:148678324
208 208 a, g dbSNP:568282645
226 226 c, t dbSNP:190132281
272 272 c, t dbSNP:557225981
308 308 c, t dbSNP:374334710
310 310 a, c dbSNP:10733689
340 340 c, t dbSNP:142220464
341 341 c, g dbSNP:10739711
360 360 c, g dbSNP:776244074
370 370 c, t dbSNP:759256668
371 371 a, g dbSNP:764847501
384 384 c, t dbSNP:116557267
385 385 a, g dbSNP:10739712
390 390 c, t dbSNP:763866831
391 391 a, g, t dbSNP:151207021
396 396 c, g dbSNP:781268651
404 404 c, t dbSNP:745980061
405 405 a, g dbSNP:575463588
426 426 c, t dbSNP:780203863
439 439 a, g dbSNP:375244764
445 445 a, c, t dbSNP:544466699
448 448 c, t dbSNP:748694702
454 454 c, t dbSNP:772659361
457 457 a, g dbSNP:773835246
459 459 a, t dbSNP:759009151
461 461 c, t dbSNP:564364290
462 462 c, g dbSNP:775067276
464 464 c, t dbSNP:762611855
467 467 a, c, t dbSNP:763883340
469 469 c, t dbSNP:757020221
471 471 -, ag dbSNP:780159749
473 473 a, g dbSNP:767479933
476 476 a, g dbSNP:200867620
478 478 a, g dbSNP:756206289
479 479 a, g dbSNP:780122824
482 482 a, c dbSNP:749474220
483 483 a, g, t dbSNP:755208661
484 484 a, g dbSNP:369778912
486 486 c, t dbSNP:748573997
487 487 a, g dbSNP:772534505
489 489 a, g dbSNP:115942384
499 499 a, g dbSNP:747542380
501 501 a, c dbSNP:769205196
510 510 c, t dbSNP:775001565
513 513 a, g dbSNP:762547418
524 524 c, t dbSNP:768345049
532 532 c, g dbSNP:773959492
536 536 c, t dbSNP:761659500
542 542 a, g dbSNP:202071258
552 552 g, t dbSNP:750341113
554 554 c, t dbSNP:377523275
555 555 a, g dbSNP:559673656
559 559 a, g dbSNP:144326872
561 561 a, g, t dbSNP:182524252
563 563 c, t dbSNP:752964984
571 571 c, t dbSNP:758894846
572 572 a, g dbSNP:568258259
582 582 c, g, t dbSNP:368885889
588 588 a, g dbSNP:781525661
602 602 g, t dbSNP:748832899
606 606 c, g dbSNP:768146873
608 608 g, t dbSNP:774044717
614 614 a, g dbSNP:373078692
615 615 c, g, t dbSNP:771768229
616 616 a, c dbSNP:202020978
659 659 a, t dbSNP:771289854
661 661 a, g dbSNP:776822595
684 684 a, g dbSNP:760114660
688 688 a, g dbSNP:148566637
699 699 c, g dbSNP:138425637
711 711 a, c dbSNP:373725272
717 717 a, t dbSNP:761463295
718 718 a, g dbSNP:201454954
721 721 -, ggtgtttaa dbSNP:760359903
725 725 -, t dbSNP:34104513
748 748 a, g dbSNP:750100888
749 749 a, t dbSNP:755784724
756 756 c, t dbSNP:35473740
757 757 a, g dbSNP:758293495
759 759 a, g dbSNP:777854337
764 764 c, t dbSNP:747143850
765 765 a, g dbSNP:757331567
766 766 c, t dbSNP:370288653
767 767 a, g, t dbSNP:558972121
779 779 a, g dbSNP:770053771
788 788 a, g dbSNP:775882793
789 789 a, c, t dbSNP:771182815
790 790 a, g dbSNP:539274578
792 792 a, g dbSNP:760327859
793 793 a, t dbSNP:144383132
794 794 c, t dbSNP:776161764
811 811 a, c, t dbSNP:759162104
812 812 c, t dbSNP:752441598
830 830 a, t dbSNP:758170699
831 831 a, g dbSNP:746022262
838 838 a, g dbSNP:751498139
843 843 a, g dbSNP:755406713
844 844 c, t dbSNP:752496326
854 854 c, t dbSNP:748634777
855 855 a, g dbSNP:772583995
859 859 c, t dbSNP:748220703
860 860 a, g dbSNP:146596005
862 862 c, t dbSNP:574131158
865 865 c, t dbSNP:769292052
866 866 c, g dbSNP:142703551
869 869 c, t dbSNP:7859709
870 870 a, c, g, t dbSNP:369537472
878 878 c, t dbSNP:767528105
879 879 a, g dbSNP:750322632
908 908 c, t dbSNP:576891746
909 909 a, g dbSNP:151146132
918 918 a, g dbSNP:781364472
923 923 c, g, t dbSNP:754116133
924 924 a, g dbSNP:765719372
927 927 a, g dbSNP:753156452
929 929 c, t dbSNP:758660440
933 933 c, t dbSNP:778414541
952 952 c, t dbSNP:747459916
973 973 a, g dbSNP:752115914
977 977 a, g dbSNP:201631831
979 979 c, t dbSNP:370988341
980 980 a, g dbSNP:748832966
983 983 c, t dbSNP:754478157
986 986 a, g dbSNP:201869990
1004 1004 a, g dbSNP:747696280
1015 1015 c, t dbSNP:771904007
1016 1016 g, t dbSNP:772842561
1034 1034 c, g dbSNP:557639053
1035 1035 a, c dbSNP:771041847
1037 1037 g, t dbSNP:776680157
1061 1061 c, t dbSNP:199564503
1062 1062 a, g dbSNP:765220171
1063 1063 c, t dbSNP:140086444
1073 1073 c, t dbSNP:763022200
1078 1078 c, t dbSNP:372542969
1079 1079 a, g dbSNP:771071856
1081 1081 c, g dbSNP:751914863
1097 1097 c, t dbSNP:762130155
1102 1102 c, t dbSNP:768077378
1103 1103 a, g dbSNP:750914983
1125 1125 c, g, t dbSNP:150336698
1126 1126 a, g dbSNP:372118470
1130 1130 -, caa dbSNP:749342875
1133 1133 a, c dbSNP:781070275
1137 1137 a, g dbSNP:745836070
1138 1138 g, t dbSNP:766359255
1139 1139 a, g dbSNP:769573706
1144 1144 c, t dbSNP:753817818
1145 1145 c, t dbSNP:375402805
1146 1146 a, g dbSNP:780007071
1157 1157 c, t dbSNP:749392034
1158 1158 a, g dbSNP:755086874
1159 1159 c, t dbSNP:369203375
1161 1161 a, c dbSNP:748301336
1169 1169 c, t dbSNP:373056648
1171 1171 a, g dbSNP:144936572
1174 1174 a, g dbSNP:761079227
1178 1178 a, c dbSNP:766835860
1179 1179 a, g dbSNP:542635849
1185 1185 a, g dbSNP:762430803
1193 1193 c, g dbSNP:376606350
1197 1197 c, t dbSNP:370591194
1198 1198 a, g dbSNP:201242773
1204 1204 a, g dbSNP:767446116
1213 1213 a, g dbSNP:146433346
1215 1215 a, g dbSNP:750278943
1229 1229 a, c, t dbSNP:755929446
1233 1233 a, g dbSNP:749188523
1235 1235 a, g dbSNP:755015821
1241 1241 c, t dbSNP:778922590
1246 1246 a, g dbSNP:544724772
1250 1250 c, t dbSNP:772329874
1259 1259 c, t dbSNP:773391642
1273 1273 a, g dbSNP:770459495
1281 1281 g, t dbSNP:556095628
1293 1293 a, g dbSNP:371529907
1296 1296 a, t dbSNP:771644764
1304 1304 a, g dbSNP:772756499
1310 1310 c, t dbSNP:760327411
1311 1311 a, g dbSNP:575643847
1320 1320 a, g dbSNP:753708155
1323 1323 a, g dbSNP:759304684
1328 1328 g, t dbSNP:765217256
1329 1329 g, t dbSNP:752625486
1331 1331 c, g dbSNP:758475931
1346 1346 c, t dbSNP:777849436
1347 1347 a, g dbSNP:751749247
1355 1355 c, t dbSNP:757458201
1368 1368 a, g dbSNP:781236718
1386 1386 c, t dbSNP:142204445
1387 1387 a, g dbSNP:201422874
1389 1389 a, t dbSNP:779266773
1393 1393 c, t dbSNP:370055267
1394 1394 a, g dbSNP:372688826
1398 1398 c, t dbSNP:776350434
1399 1399 a, g dbSNP:145988246
1412 1412 c, g dbSNP:140777921
1415 1415 c, t dbSNP:775223622
1425 1425 c, t dbSNP:762703146
1431 1431 c, t dbSNP:764138101
1433 1433 c, t dbSNP:569190005
1442 1442 g, t dbSNP:761962113
1444 1444 c, t dbSNP:200130106
1451 1451 c, g dbSNP:767442589
1454 1454 a, g dbSNP:750699865
1457 1457 a, c, g dbSNP:531778526
1458 1458 c, g, t dbSNP:754304263
1461 1461 a, g dbSNP:779540699
1466 1466 c, t dbSNP:748536660
1469 1469 a, c, g dbSNP:142900894
1474 1474 a, g dbSNP:745548531
1481 1481 c, t dbSNP:769473901
1486 1486 c, t dbSNP:775097320
1487 1487 c, t dbSNP:377362242
1501 1501 c, g dbSNP:768273167
1502 1502 c, t dbSNP:774362337
1505 1505 -, c dbSNP:773599471
1505 1505 c, t dbSNP:144905207
1506 1506 a, g dbSNP:767669209
1508 1508 c, t dbSNP:773467573
1518 1518 a, g dbSNP:760816369
1519 1519 a, c, g dbSNP:367599861
1523 1523 c, t dbSNP:571794213
1544 1544 a, g, t dbSNP:527757590
1556 1556 c, t dbSNP:148311002
1562 1562 a, g dbSNP:369981036
1568 1568 c, g dbSNP:764639834
1571 1571 c, t dbSNP:752053223
1584 1584 a, g dbSNP:755638307
1586 1586 a, g dbSNP:141401867
1587 1587 a, g dbSNP:753561842
1592 1592 c, t dbSNP:754630387
1593 1593 a, g dbSNP:778606816
1596 1596 a, c, g dbSNP:747954096
1601 1601 c, t dbSNP:542867942
1604 1604 a, g dbSNP:746883992
1613 1613 c, g, t dbSNP:771156426
1616 1616 c, t dbSNP:759628591
1623 1623 a, g dbSNP:769955139
1629 1629 a, g, t dbSNP:775766266
1655 1655 -, c dbSNP:770577173
1658 1658 c, g dbSNP:764372216
1676 1676 c, t dbSNP:752085981
1678 1678 a, g dbSNP:762271253
1686 1686 a, g dbSNP:767983433
1687 1687 c, t dbSNP:753341608
1688 1688 a, g dbSNP:547576840
1700 1700 c, t dbSNP:369405997
1705 1705 a, g dbSNP:751448393
1706 1706 c, t dbSNP:150859583
1709 1709 c, t dbSNP:781056301
1711 1711 c, t dbSNP:373230180
1712 1712 a, g dbSNP:16930267
1719 1719 a, g dbSNP:780145419
1726 1726 a, g dbSNP:749338871
1727 1727 a, c dbSNP:768918046
1730 1730 c, t dbSNP:371151024
1744 1744 c, t dbSNP:201558106
1749 1749 c, t dbSNP:374458248
1750 1750 a, g dbSNP:772416779
1752 1752 a, g dbSNP:773606820
1762 1762 c, t dbSNP:142830945
1764 1764 c, t dbSNP:554406399
1766 1766 c, t dbSNP:775178382
1776 1776 a, g dbSNP:762527775
1791 1791 a, c dbSNP:763894403
1795 1795 a, t dbSNP:375698904
1797 1797 a, g dbSNP:761513312
1798 1798 c, t dbSNP:767280977
1808 1808 a, c dbSNP:750361886
1821 1821 a, g dbSNP:756121970
1828 1828 a, g dbSNP:779836096
1829 1829 c, t dbSNP:111440458
1830 1830 a, g dbSNP:755047936
1835 1835 c, t dbSNP:779114460
1838 1838 c, t dbSNP:748311616
1839 1839 a, g dbSNP:146124484
1851 1851 a, g dbSNP:772346771
1853 1853 c, t dbSNP:778158911
1856 1856 c, t dbSNP:747304394
1857 1857 c, g dbSNP:771515643
1860 1860 a, g dbSNP:777263284
1862 1862 c, t dbSNP:762615145
1863 1863 a, g dbSNP:370283153
1871 1871 a, g dbSNP:774155897
1873 1873 a, g dbSNP:199739089
1878 1878 -, g dbSNP:759027068
1878 1878 c, t dbSNP:369962505
1879 1879 a, g, t dbSNP:201844320
1879 1879 -, g dbSNP:765268245
1881 1881 a, c, g, t dbSNP:200194334
1884 1884 a, g, t dbSNP:201248373
1885 1885 a, c, g dbSNP:577175137
1890 1890 c, t dbSNP:752894376
1891 1891 a, g dbSNP:377049698
1894 1894 a, c, t dbSNP:370271137
1895 1895 a, g dbSNP:374588749
1907 1907 a, g dbSNP:781764705
1908 1908 c, g dbSNP:746317417
1920 1920 c, t dbSNP:768300868
1921 1921 a, g dbSNP:199878817
1925 1925 a, c, g, t dbSNP:775510321
1926 1926 c, t dbSNP:760523646
1927 1927 c, t dbSNP:141910331
1950 1950 g, t dbSNP:547868115
1977 1977 c, t dbSNP:561255124
1986 1986 c, t dbSNP:530300344
2004 2004 c, t dbSNP:745933780
2008 2008 c, t dbSNP:770024989
2009 2009 a, g dbSNP:75511005
2031 2031 a, g dbSNP:186440107
2033 2033 a, c dbSNP:760534129
2049 2049 a, c dbSNP:532623293
2052 2052 c, t dbSNP:13298797
2054 2054 a, g dbSNP:552462679
2073 2073 a, g dbSNP:565329443
2084 2084 c, g, t dbSNP:114790720
2131 2131 c, t dbSNP:541860686
2137 2137 c, t dbSNP:780375217
2148 2148 a, g dbSNP:372694601
2177 2177 c, t dbSNP:7872702
2178 2178 a, g dbSNP:140738643
2196 2196 c, t dbSNP:577113755
2216 2216 c, t dbSNP:374091374
2241 2241 c, t dbSNP:10987876
2242 2242 a, c, g dbSNP:188541776
2263 2263 c, t dbSNP:369881030
2264 2264 a, g dbSNP:111828101
2267 2267 c, g dbSNP:572383902
2287 2287 -, ctgc dbSNP:567835255
2294 2294 a, c dbSNP:750834588
2306 2306 a, g dbSNP:541101417
2333 2333 a, c dbSNP:376171587
2372 2372 a, c dbSNP:369199483
2390 2390 c, g dbSNP:561350806
2412 2412 c, g dbSNP:3814524
2425 2425 a, g dbSNP:760444702
2476 2476 c, t dbSNP:767020937
2489 2489 c, t dbSNP:543515646
2533 2533 c, t dbSNP:564699238
2560 2560 -, g dbSNP:34224940
2610 2610 a, g dbSNP:770879666
2628 2628 a, c dbSNP:757971886
2638 2638 c, t dbSNP:563501308
2645 2645 a, g dbSNP:532609821
2657 2657 a, g dbSNP:552446244
2683 2683 c, t dbSNP:566269410
2684 2684 a, g dbSNP:746741415
2689 2689 a, g dbSNP:756864174
2692 2692 c, g dbSNP:528071747
2695 2695 a, c dbSNP:116513398
2704 2704 a, g dbSNP:180947406
2734 2734 a, g dbSNP:537095783
2735 2735 a, g dbSNP:185116755
2738 2738 g, t dbSNP:745683615
2783 2783 c, t dbSNP:57052978
2785 2785 a, g dbSNP:769689731
2821 2821 c, t dbSNP:147539248
2822 2822 -, gag dbSNP:567976888
2824 2824 a, g dbSNP:775457386
2864 2864 a, c, t dbSNP:749315548
2892 2892 c, t dbSNP:139384794
2899 2899 a, g dbSNP:536517546
2976 2976 a, t dbSNP:774303940
2979 2979 -, ctaa dbSNP:528213954
2980 2980 -, ctaa dbSNP:777101904
2983 2983 -, ctaa dbSNP:773891780
2995 2995 c, g dbSNP:542129619
3030 3030 c, t dbSNP:191060669
3121 3121 c, t dbSNP:764745985
3145 3145 c, g dbSNP:767657703
3176 3176 c, t dbSNP:773668033
3177 3177 a, g dbSNP:574788142
3202 3202 -, t dbSNP:35604217
3255 3255 a, t dbSNP:537978043
3267 3267 c, t dbSNP:761158288
3268 3268 a, g dbSNP:766965728
3299 3299 g, t dbSNP:374692425
3325 3325 c, t dbSNP:532543390
3351 3351 c, t dbSNP:546018894
3365 3365 a, t dbSNP:559910298
3394 3394 c, t dbSNP:528751634
3405 3405 a, g dbSNP:757840839
3426 3426 c, g dbSNP:369941858
3429 3429 a, c, g dbSNP:10760538
3431 3431 -, g dbSNP:35358699
3568 3568 c, t dbSNP:561483406
3597 3597 a, g dbSNP:35037027
3606 3606 a, c dbSNP:751043182
3626 3626 c, t dbSNP:530560615
3634 3634 a, g dbSNP:184011703
3672 3672 a, g dbSNP:77244294

Target ORF information:

RefSeq Version NM_001006642
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 (SLC25A25), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001006642

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTTGCAGA TGCTGTGGCA TTTCCTAGCT AGCTTTTTCC CCAGGGCTGG GTGCCACGGC 
TCCAGAGAGG GGGACGATCG TGAAGTCAGA GGCACCCCAG CCCCTGCCTG GAGAGACCAG
ATGGCAAGCT TTTTGGGGAA ACAGGACGGA AGGGCTGAGG CCACGGAAAA AAGACCCACC
ATTTTGCTGG TGGTTGGACC TGCAGAGCAG TTTCCTAAGA AAATTGTACA AGCTGGAGAT
AAGGACCTTG ATGGGCAGCT AGACTTTGAA GAATTTGTCC ATTATCTCCA AGATCATGAG
AAGAAGCTGA GGCTGGTGTT TAAGAGTTTG GACAAAAAGA ATGATGGACG CATTGACGCG
CAGGAGATCA TGCAGTCCCT GCGGGACTTG GGAGTCAAGA TATCTGAACA GCAGGCAGAA
AAAATTCTCA AGAGCATGGA TAAAAACGGC ACGATGACCA TCGACTGGAA CGAGTGGAGA
GACTACCACC TCCTCCACCC CGTGGAAAAC ATCCCCGAGA TCATCCTCTA CTGGAAGCAT
TCCACGATCT TTGATGTGGG TGAGAATCTA ACGGTCCCGG ATGAGTTCAC AGTGGAGGAG
AGGCAGACGG GGATGTGGTG GAGACACCTG GTGGCAGGAG GTGGGGCAGG GGCCGTATCC
AGAACCTGCA CGGCCCCCCT GGACAGGCTC AAGGTGCTCA TGCAGGTCCA TGCCTCCCGC
AGCAACAACA TGGGCATCGT TGGTGGCTTC ACTCAGATGA TTCGAGAAGG AGGGGCCAGG
TCACTCTGGC GGGGCAATGG CATCAACGTC CTCAAAATTG CCCCCGAATC AGCCATCAAA
TTCATGGCCT ATGAGCAGAT CAAGCGCCTT GTTGGTAGTG ACCAGGAGAC TCTGAGGATT
CACGAGAGGC TTGTGGCAGG GTCCTTGGCA GGGGCCATCG CCCAGAGCAG CATCTACCCA
ATGGAGGTCC TGAAGACCCG GATGGCGCTG CGGAAGACAG GCCAGTACTC AGGAATGCTG
GACTGCGCCA GGAGGATCCT GGCCAGAGAG GGGGTGGCCG CCTTCTACAA AGGCTATGTC
CCCAACATGC TGGGCATCAT CCCCTATGCC GGCATCGACC TTGCAGTCTA CGAGACGCTC
AAGAATGCCT GGCTGCAGCA CTATGCAGTG AACAGCGCGG ACCCCGGCGT GTTTGTGCTC
CTGGCCTGTG GCACCATGTC CAGTACCTGT GGCCAGCTGG CCAGCTACCC CCTGGCCCTA
GTCAGGACCC GGATGCAGGC GCAAGCCTCT ATTGAGGGCG CTCCGGAGGT GACCATGAGC
AGCCTCTTCA AACATATCCT GCGGACCGAG GGGGCCTTCG GGCTGTACAG GGGGCTGGCC
CCCAACTTCA TGAAGGTCAT CCCAGCTGTG AGCATCAGCT ACGTGGTCTA CGAGAACCTG
AAGATCACCC TGGGCGTGCA GTCGCGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu12847
Accession Version NM_001265614.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product calcium-binding mitochondrial carrier protein SCaMC-2 isoform 5 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB067483.1, DA219774.1, AL360268.14, AJ619990.1 and BM689843.1. On Jul 11, 2012 this sequence version replaced gi:388453840. Summary: The protein encoded by this gene belongs to the family of calcium-binding mitochondrial carriers, with a characteristic mitochondrial carrier domain at the C-terminus. These proteins are found in the inner membranes of mitochondria, and function as transport proteins. They shuttle metabolites, nucleotides and cofactors through the mitochondrial membrane and thereby connect and/or regulate cytoplasm and matrix functions. This protein may function as an ATP-Mg/Pi carrier that mediates the transport of Mg-ATP in exchange for phosphate, and likely responsible for the net uptake or efflux of adenine nucleotides into or from the mitochondria. Alternatively spliced transcript variants encoding different isoforms with a common C-terminus but variable N-termini have been described for this gene. [provided by RefSeq, Jul 2012]. Transcript Variant: This variant (5) contains an alternate 5' terminal exon, and an additional in-frame coding exon compared to variant 1. It initiates tran