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TMC1 transmembrane channel-like 1 [Homo sapiens (human)]

Gene Symbol TMC1
Entrez Gene ID 117531
Full Name transmembrane channel-like 1
Synonyms DFNA36, DFNB11, DFNB7
General protein information
Preferred Names
transmembrane channel-like protein 1
Names
transmembrane channel-like protein 1
transmembrane, cochlear expressed, 1
transmembrane cochlear-expressed protein 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

9

9q21.12

Summary This gene is considered a member of a gene family predicted to encode transmembrane proteins. The specific function of this gene is unknown; however, it is known to be required for normal function of cochlear hair cells. Mutations in this gene have been associated with progressive postlingual hearing loss and profound prelingual deafness. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

606706

Disorder Html: Deafness, autosomal recessive 7, 600974 (3); Deafness, autosomal





The following TMC1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMC1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu25940 NM_138691 Homo sapiens transmembrane channel-like 1 (TMC1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$769
OHu75047 XM_011518213 PREDICTED: Homo sapiens transmembrane channel-like 1 (TMC1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu25940
Accession Version NM_138691.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear Document: OHu25940D_COA.pdf (pdf)
Update Date 24-MAY-2014
Organism Homo sapiens (human)
Product transmembrane channel-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF417578.1. This sequence is a reference standard in the RefSeqGene project. On May 22, 2002 this sequence version replaced gi:20162551. Summary: This gene is considered a member of a gene family predicted to encode transmembrane proteins. The specific function of this gene is unknown; however, it is known to be required for normal function of cochlear hair cells. Mutations in this gene have been associated with progressive postlingual hearing loss and profound prelingual deafness. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF417578.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTCACCCA AAAAAGTACA AATCAAAGTG GAGGAAAAAG AAGACGAGAC TGAGGAAAGC 
TCAAGTGAAG AGGAAGAGGA GGTGGAAGAT AAGCTACCTC GAAGAGAGAG CTTGAGACCA
AAGAGGAAAC GGACCAGAGA TGTTATCAAT GAGGATGACC CAGAACCTGA ACCAGAGGAT
GAAGAAACAA GGAAGGCAAG AGAAAAAGAG AGGAGGAGGA GGCTAAAGAG AGGAGCAGAA
GAAGAAGAAA TTGATGAAGA GGAATTGGAA AGATTGAAGG CAGAGTTAGA TGAGAAAAGA
CAAATAATTG CTACTGTCAA ATGCAAACCA TGGAAGATGG AGAAGAAAAT TGAAGTTCTC
AAGGAGGCAA AAAAATTTGT GAGTGAAAAT GAAGGGGCTC TTGGGAAAGG AAAAGGAAAA
CGGTGGTTTG CATTTAAGAT GATGATGGCC AAGAAATGGG CAAAATTCCT CCGTGATTTT
GAGAACTTCA AAGCTGCGTG TGTCCCATGG GAAAATAAAA TCAAGGCTAT TGAAAGTCAG
TTTGGCTCCT CAGTGGCCTC ATACTTCCTC TTCTTGAGAT GGATGTATGG AGTCAATATG
GTTCTCTTTA TCCTGACATT TAGCCTCATC ATGTTGCCAG AGTACCTCTG GGGTTTGCCA
TATGGCAGTT TACCTAGGAA AACCGTTCCC AGAGCCGAAG AGGCATCGGC AGCAAACTTT
GGTGTGTTGT ACGACTTCAA TGGTTTGGCA CAATATTCCG TTCTCTTTTA TGGCTATTAT
GACAATAAAC GAACAATTGG ATGGATGAAT TTCAGGTTGC CGCTCTCCTA TTTTCTAGTG
GGGATTATGT GCATTGGATA CAGCTTTCTG GTTGTCCTCA AAGCAATGAC CAAAAACATT
GGTGATGATG GAGGTGGAGA TGACAACACT TTCAATTTCA GCTGGAAGGT CTTTACCAGC
TGGGACTACC TGATCGGCAA TCCTGAAACA GCAGACAACA AATTTAATTC TATCACAATG
AACTTTAAGG AAGCTATCAC AGAAGAAAAA GCAGCCCAAG TAGAAGAAAA CGTCCACTTG
ATCAGATTCC TGAGGTTTCT GGCTAACTTC TTCGTGTTTC TAACACTTGG AGGGAGTGGA
TACCTCATCT TTTGGGCTGT GAAGCGATCC CAGGAATTTG CACAGCAAGA TCCTGACACC
CTTGGGTGGT GGGAAAAAAA TGAAATGAAC ATGGTTATGT CCCTCCTAGG GATGTTCTGT
CCAACATTGT TTGACTTATT TGCTGAATTA GAAGACTACC ATCCTCTCAT CGCTTTGAAA
TGGCTACTGG GACGCATTTT TGCTCTTCTT TTAGGCAATT TATACGTATT TATTCTTGCA
TTAATGGATG AGATTAACAA CAAGATTGAA GAGGAGAAGC TAGTAAAGGC CAATATTACC
CTTTGGGAAG CCAATATGAT CAAGGCCTAC AATGCATCAT TCTCTGAAAA TAGCACTGGA
CCACCCTTTT TTGTTCACCC TGCAGATGTA CCTCGAGGAC CTTGCTGGGA AACAATGGTG
GGACAGGAGT TTGTGAGGCT GACAGTCTCT GATGTTCTGA CCACCTACGT CACAATCCTC
ATTGGGGACT TTCTAAGGGC ATGTTTTGTG AGGTTTTGCA ATTATTGCTG GTGCTGGGAC
TTGGAGTATG GATATCCTTC ATACACCGAA TTCGACATCA GTGGCAACGT CCTCGCTCTG
ATCTTCAACC AAGGCATGAT CTGGATGGGC TCCTTCTTTG CTCCCAGCCT CCCAGGCATC
AATATCCTTC GACTCCATAC ATCCATGTAC TTCCAGTGCT GGGCCGTTAT GTGCTGCAAT
GTTCCTGAGG CCAGGGTCTT CAAAGCTTCC AGATCAAATA ACTTCTACCT GGGCATGCTA
CTGCTCATCC TCTTCCTGTC CACAATGCCT GTCTTGTACA TGATCGTGTC CCTCCCACCA
TCTTTTGATT GTGGTCCATT CAGTGGCAAA AATAGAATGT TTGAAGTCAT TGGAGAGACC
CTGGAGCACG ATTTCCCAAG CTGGATGGCG AAGATCTTGA GACAGCTTTC AAACCCTGGG
CTGGTCATTG CTGTCATTTT GGTGATGGTT TTGGCCATCT ATTATCTCAA TGCTACTGCC
AAGGGCCAGA AGGCAGCGAA TCTGGATCTC AAAAAGAAGA TGAAAATGCA AGCTTTGGAG
AACAAAATGC GAAACAAGAA AATGGCAGCT GCACGAGCAG CTGCAGCTGC TGGTCGCCAG
TAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_619636.2
CDS541..2823
Misc Feature(1)523..525(+)
Misc Feature(2)1138..1200(+)
Misc Feature(3)1357..1419(+)
Misc Feature(4)1636..1698(+)
Misc Feature(5)1861..1923(+)
Misc Feature(6)2083..2430(+)
Misc Feature(7)2443..2505(+)
Misc Feature(8)2638..2700(+)
Exon (1)1..113
Gene:TMC1
Gene Synonym:
Exon (2)114..235
Gene:TMC1
Gene Synonym:
Exon (3)236..345
Gene:TMC1
Gene Synonym:
Exon (4)346..488
Gene:TMC1
Gene Synonym:
Exon (5)489..556
Gene:TMC1
Gene Synonym:
Exon (6)557..604
Gene:TMC1
Gene Synonym:
Exon (7)605..776
Gene:TMC1
Gene Synonym:
Exon (8)777..902
Gene:TMC1
Gene Synonym:
Exon (9)903..993
Gene:TMC1
Gene Synonym:
Exon (10)994..1075
Gene:TMC1
Gene Synonym:
Exon (11)1076..1182
Gene:TMC1
Gene Synonym:
Exon (12)1183..1281
Gene:TMC1
Gene Synonym:
Exon (13)1282..1424
Gene:TMC1
Gene Synonym:
Exon (14)1425..1569
Gene:TMC1
Gene Synonym:
Exon (15)1570..1764
Gene:TMC1
Gene Synonym:
Exon (16)1765..1944
Gene:TMC1
Gene Synonym:
Exon (17)1945..2106
Gene:TMC1
Gene Synonym:
Exon (18)2107..2235
Gene:TMC1
Gene Synonym:
Exon (19)2236..2303
Gene:TMC1
Gene Synonym:
Exon (20)2304..2543
Gene:TMC1
Gene Synonym:
Exon (21)2544..2669
Gene:TMC1
Gene Synonym:
Exon (22)2670..2748
Gene:TMC1
Gene Synonym:
Exon (23)2749..2800
Gene:TMC1
Gene Synonym:
Exon (24)2801..3201
Gene:TMC1
Gene Synonym:
Translation
Position Chain Variation Link
1 1 c, t dbSNP:112220638
10 10 c, t dbSNP:548405474
27 27 a, c dbSNP:562169922
60 60 c, t dbSNP:757908231
73 73 a, g dbSNP:7022441
77 77 g, t dbSNP:550923706
90 90 a, g dbSNP:759881740
119 119 a, t dbSNP:376907111
161 161 c, t dbSNP:765514226
178 178 c, t dbSNP:746537110
188 188 g, t dbSNP:560962333
212 212 a, c dbSNP:7856724
225 225 -, g dbSNP:35604642
263 263 c, t dbSNP:551007397
264 264 a, g dbSNP:550532865
266 266 a, g dbSNP:58824091
282 282 c, t dbSNP:768096961
287 287 c, t dbSNP:536163534
289 289 c, t dbSNP:541857536
301 301 c, t dbSNP:546934959
322 322 a, g dbSNP:7026304
347 347 a, t dbSNP:762135062
361 361 a, t dbSNP:371546038
373 373 c, g dbSNP:573802434
402 402 g, t dbSNP:757162804
417 417 c, t dbSNP:533837914
425 425 g, t dbSNP:768959194
430 430 a, g dbSNP:750183854
433 433 a, g dbSNP:183039764
445 445 c, t dbSNP:776867976
446 446 a, g dbSNP:758655552
483 483 g, t dbSNP:575997087
492 492 c, t dbSNP:780086356
494 494 c, t dbSNP:747134278
496 496 c, t dbSNP:768817345
503 503 a, g dbSNP:781391084
507 507 a, g dbSNP:748715363
512 512 c, t dbSNP:770418317
515 515 a, t dbSNP:773629810
519 519 c, t dbSNP:763169284
520 520 c, t dbSNP:201641451
522 522 c, t dbSNP:771779042
525 525 -, caggacac dbSNP:573607788
526 526 a, c dbSNP:774961281
527 527 a, g dbSNP:760285926
533 533 a, c, t dbSNP:564398062
533 533 -, c dbSNP:755557705
539 539 a, g dbSNP:199639327
540 540 g, t dbSNP:765021495
547 547 a, c dbSNP:569626109
549 549 -, a dbSNP:779117143
551 551 a, g dbSNP:145050019
555 555 a, t dbSNP:766655548
556 556 a, g dbSNP:751771334
560 560 -, a dbSNP:757999109
562 562 -, a dbSNP:727504554
563 563 a, t dbSNP:372383725
571 571 c, g dbSNP:757765718
584 584 a, t dbSNP:779209726
585 585 c, t dbSNP:2589615
586 586 a, g dbSNP:140437301
592 592 a, g dbSNP:776220877
599 599 c, g dbSNP:189479995
604 604 a, g, t dbSNP:144387828
618 618 a, g dbSNP:769476838
619 619 a, g dbSNP:777217789
624 624 a, g dbSNP:748771130
627 627 a, g dbSNP:770793343
629 629 a, g dbSNP:367993839
640 640 c, t dbSNP:121908073
641 641 a, g dbSNP:753977925
667 667 -, a dbSNP:777419888
670 670 c, t dbSNP:775411731
671 671 a, g dbSNP:572854741
675 675 a, c dbSNP:111353632
677 677 g, t dbSNP:753879286
681 681 a, g, t dbSNP:140388347
682 682 a, g dbSNP:562374634
685 685 a, c dbSNP:149947445
700 700 c, t dbSNP:780458439
717 717 a, g dbSNP:751950994
721 721 a, g dbSNP:755733171
733 733 a, g dbSNP:145098032
736 736 a, g dbSNP:551184500
743 743 a, g dbSNP:564668916
751 751 a, g dbSNP:779134204
759 759 -, ggagg dbSNP:730880359
767 767 a, g dbSNP:778543840
776 776 a, c, t dbSNP:140482278
777 777 -, aga dbSNP:376040866
778 778 a, g dbSNP:145647775
781 781 a, g dbSNP:1796993
783 783 a, c dbSNP:536350054
787 787 -, gaa dbSNP:397517839
833 833 a, g dbSNP:767739170
856 856 a, g dbSNP:753572224
873 873 a, g dbSNP:756957433
878 878 c, t dbSNP:778631855
879 879 a, g dbSNP:397517840
882 882 a, g dbSNP:370554600
890 890 c, t dbSNP:758421299
902 902 a, g dbSNP:368673178
906 906 a, g dbSNP:759933822
908 908 -, a dbSNP:769806797
909 909 -, a dbSNP:745767539
913 913 a, c dbSNP:377607548
916 916 a, t dbSNP:752998493
922 922 a, g dbSNP:372983741
924 924 c, t dbSNP:764922731
926 926 a, t dbSNP:749978207
943 943 a, g dbSNP:397517841
954 954 a, g dbSNP:566767136
961 961 a, c, t dbSNP:11143384
980 980 c, t dbSNP:781112060
988 988 a, g dbSNP:534560340
1000 1000 g, t dbSNP:145102585
1001 1001 c, t dbSNP:371485372
1002 1002 a, g dbSNP:539228804
1008 1008 a, c dbSNP:761235546
1012 1012 c, t dbSNP:200841002
1013 1013 a, g dbSNP:148340276
1023 1023 a, g dbSNP:375984349
1033 1033 c, g dbSNP:762736726
1037 1037 c, t dbSNP:765885670
1038 1038 a, g dbSNP:370032879
1044 1044 c, g dbSNP:758924923
1047 1047 a, g dbSNP:767019298
1049 1049 a, g dbSNP:752678337
1069 1069 a, g dbSNP:756028904
1070 1070 c, t dbSNP:747645067
1072 1072 -, a dbSNP:762864983
1076 1076 a, g dbSNP:770640925
1084 1084 a, g dbSNP:773838098
1085 1085 a, g dbSNP:199560971
1089 1089 c, t dbSNP:375128682
1096 1096 a, g dbSNP:774972622
1097 1097 c, g, t dbSNP:727504470
1125 1125 g, t dbSNP:368246679
1129 1129 a, g dbSNP:753687760
1136 1136 a, g, t dbSNP:756960425
1139 1139 c, t dbSNP:750662616
1143 1143 g, t dbSNP:372449375
1145 1145 c, t dbSNP:780348810
1146 1146 c, g dbSNP:376768305
1158 1158 a, c dbSNP:199499836
1159 1159 a, t dbSNP:368042336
1164 1164 a, c dbSNP:781747541
1167 1167 c, t dbSNP:748580616
1168 1168 a, g dbSNP:565926145
1172 1172 g, t dbSNP:773609760
1177 1177 c, t dbSNP:745569653
1191 1191 g, t dbSNP:150896910
1196 1196 c, t dbSNP:749608265
1198 1198 a, c dbSNP:771735027
1201 1201 c, t dbSNP:779639998
1202 1202 a, g dbSNP:533159785
1214 1214 c, t dbSNP:370088722
1218 1218 c, g dbSNP:776193666
1225 1225 a, g dbSNP:201827013
1236 1236 c, t dbSNP:112843964
1237 1237 a, g dbSNP:373537563
1242 1242 a, g dbSNP:762790929
1243 1243 g, t dbSNP:200831684
1245 1245 a, g dbSNP:560655583
1247 1247 c, t dbSNP:751873680
1248 1248 a, g dbSNP:759815339
1256 1256 a, g dbSNP:767557406
1262 1262 a, g dbSNP:752751599
1275 1275 c, t dbSNP:143404572
1278 1278 c, g dbSNP:778293772
1280 1280 a, g dbSNP:549050367
1285 1285 a, t dbSNP:750724247
1299 1299 c, t dbSNP:143200611
1300 1300 a, g dbSNP:111033497
1301 1301 c, t dbSNP:375919123
1305 1305 c, t dbSNP:201230747
1315 1315 c, t dbSNP:764050135
1317 1317 c, t dbSNP:727503482
1320 1320 c, t dbSNP:754390601
1326 1326 c, t dbSNP:780868794
1330 1330 c, t dbSNP:765657443
1331 1331 a, c, g dbSNP:147147941
1335 1335 a, c dbSNP:140398130
1336 1336 a, g dbSNP:536753857
1337 1337 c, t dbSNP:747645756
1343 1343 g, t dbSNP:750373958
1354 1354 a, g dbSNP:111839361
1361 1361 c, t dbSNP:755694066
1362 1362 a, g dbSNP:370523728
1365 1365 c, t dbSNP:749302129
1367 1367 a, c dbSNP:770921263
1370 1370 -, t dbSNP:752999399
1370 1370 a, g dbSNP:774299359
1380 1380 -, g dbSNP:758763154
1381 1381 g, t dbSNP:397517842
1390 1390 c, t dbSNP:745607141
1419 1419 c, g, t dbSNP:772213890
1424 1424 c, g, t dbSNP:760785189
1429 1429 a, t dbSNP:776707718
1433 1433 a, g dbSNP:149912546
1441 1441 a, g dbSNP:770320624
1442 1442 c, g dbSNP:773751182
1446 1446 g, t dbSNP:763351588
1447 1447 g, t dbSNP:766828978
1463 1463 -, aca dbSNP:778174231
1464 1464 c, g, t dbSNP:113342704
1468 1468 a, g dbSNP:144501871
1475 1475 a, g dbSNP:753426697
1476 1476 g, t dbSNP:148443938
1496 1496 c, g dbSNP:142629075
1497 1497 c, g, t dbSNP:750432713
1500 1500 -, c dbSNP:751195625
1504 1504 g, t dbSNP:779905040
1508 1508 a, g dbSNP:746724027
1520 1520 a, t dbSNP:768891360
1524 1524 c, t dbSNP:781353378
1527 1527 a, c dbSNP:748342146
1535 1535 a, g dbSNP:769913872
1542 1542 a, g dbSNP:773156746
1550 1550 a, c dbSNP:376305043
1558 1558 a, g dbSNP:771281791
1559 1559 c, t dbSNP:111033464
1562 1562 a, c dbSNP:727504568
1579 1579 a, g dbSNP:749274559
1591 1591 a, g dbSNP:771497286
1610 1610 a, g dbSNP:397517834
1612 1612 a, c, g dbSNP:774756982
1618 1618 a, t dbSNP:772620520
1624 1624 a, g dbSNP:775992461
1625 1625 a, g dbSNP:761560850
1630 1630 c, t dbSNP:764926506
1640 1640 g, t dbSNP:768955462
1651 1651 c, t dbSNP:758739636
1653 1653 c, t dbSNP:762332645
1654 1654 a, g dbSNP:367924428
1662 1662 a, g dbSNP:751503196
1669 1669 a, g dbSNP:754899734
1670 1670 a, g dbSNP:767369935
1672 1672 a, g dbSNP:752427037
1679 1679 a, g dbSNP:372215112
1680 1680 a, c dbSNP:777814216
1681 1681 a, t dbSNP:749491943
1683 1683 a, c dbSNP:757327146
1686 1686 c, t dbSNP:779035669
1705 1705 c, t dbSNP:151001642
1706 1706 a, g dbSNP:772640673
1712 1712 a, c dbSNP:776010914
1720 1720 g, t dbSNP:374313036
1722 1722 a, g dbSNP:769519448
1725 1725 a, g dbSNP:371815403
1729 1729 a, g dbSNP:185702148
1739 1739 c, g dbSNP:765865619
1749 1749 c, g dbSNP:773851192
1752 1752 c, g dbSNP:759478413
1755 1755 a, c dbSNP:767390037
1761 1761 -, t dbSNP:769571760
1773 1773 a, g dbSNP:184603559
1774 1774 c, g dbSNP:568058201
1782 1782 c, t dbSNP:371854262
1791 1791 a, g dbSNP:771536758
1792 1792 a, g dbSNP:775453038
1793 1793 a, t dbSNP:786201027
1796 1796 c, t dbSNP:760645934
1803 1803 a, g dbSNP:139985214
1807 1807 c, t dbSNP:753600914
1808 1808 g, t dbSNP:761954491
1815 1815 c, t dbSNP:765507648
1819 1819 c, t dbSNP:750563026
1824 1824 c, t dbSNP:758592011
1825 1825 a, g dbSNP:780260906
1833 1833 a, c dbSNP:752172170
1841 1841 a, c dbSNP:375264737
1842 1842 a, c, t dbSNP:781418962
1849 1849 a, c dbSNP:770517350
1851 1851 c, t dbSNP:537381495
1852 1852 a, g dbSNP:745495139
1869 1869 a, g dbSNP:369197132
1871 1871 a, g dbSNP:771625117
1873 1873 c, t dbSNP:372710475
1874 1874 a, g dbSNP:760532554
1888 1888 c, t dbSNP:768579412
1891 1891 g, t dbSNP:776311501
1899 1899 c, t dbSNP:761629265
1902 1902 a, g dbSNP:765094337
1905 1905 c, t dbSNP:750724544
1906 1906 a, g dbSNP:763255951
1909 1909 c, t dbSNP:397517836
1915 1915 c, t dbSNP:751594947
1925 1925 c, t dbSNP:755457390
1927 1927 a, g dbSNP:758352261
1936 1936 -, aac dbSNP:780156287
1943 1943 a, g dbSNP:753108960
1959 1959 a, c, g dbSNP:371004715
1960 1960 c, t dbSNP:756605797
1968 1968 a, g dbSNP:373847743
1984 1984 c, t dbSNP:754142954
1994 1994 a, g dbSNP:758068479
1997 1997 c, t dbSNP:17058153
2000 2000 g, t dbSNP:746611422
2006 2006 c, t dbSNP:368087393
2015 2015 c, t dbSNP:780555614
2017 2017 a, t dbSNP:748118938
2018 2018 c, t dbSNP:769847194
2025 2025 g, t dbSNP:370302201
2038 2038 a, g dbSNP:773195404
2039 2039 a, g dbSNP:749066542
2042 2042 c, t dbSNP:771313896
2044 2044 c, t dbSNP:774660132
2072 2072 a, c dbSNP:727503483
2074 2074 c, t dbSNP:200171616
2083 2083 c, t dbSNP:121908076
2084 2084 g, t dbSNP:767642609
2102 2102 g, t dbSNP:146558576
2103 2103 a, g dbSNP:761230185
2106 2106 a, g dbSNP:372344935
2110 2110 c, t dbSNP:777145557
2124 2124 a, c dbSNP:762248733
2126 2126 a, t dbSNP:761621035
2128 2128 c, t dbSNP:750746559
2134 2134 a, g dbSNP:759289124
2142 2142 c, g dbSNP:111033462
2148 2148 c, t dbSNP:552170649
2149 2149 a, g dbSNP:150206751
2151 2151 c, t dbSNP:777364081
2160 2160 c, t dbSNP:145772877
2162 2162 c, t dbSNP:201955337
2166 2166 a, g dbSNP:778829243
2169 2169 c, t dbSNP:745630603
2172 2172 c, t dbSNP:772378812
2188 2188 g, t dbSNP:201465054
2192 2192 a, g dbSNP:747186037
2198 2198 g, t dbSNP:768736139
2200 2200 a, t dbSNP:776624164
2218 2218 a, g dbSNP:762371411
2219 2219 a, t dbSNP:770186250
2220 2220 c, t dbSNP:773672736
2232 2232 a, t dbSNP:763192082
2245 2245 a, g dbSNP:148971770
2247 2247 c, g dbSNP:760464772
2248 2248 a, g dbSNP:369890353
2253 2253 c, t dbSNP:34532421
2254 2254 a, c, g dbSNP:121908072
2268 2268 c, t dbSNP:761261855
2274 2274 c, t dbSNP:374323593
2282 2282 g, t dbSNP:764756492
2283 2283 a, c dbSNP:750341293
2290 2290 a, c dbSNP:758288878
2291 2291 a, g dbSNP:377731576
2302 2302 c, t dbSNP:766228632
2304 2304 a, g dbSNP:368084452
2308 2308 a, g dbSNP:759355645
2313 2313 c, t dbSNP:767247084
2325 2325 c, t dbSNP:752871843
2334 2334 a, g dbSNP:756359010
2336 2336 a, g dbSNP:727503486
2338 2338 a, g dbSNP:202144745
2350 2350 c, t dbSNP:777777359
2354 2354 c, t dbSNP:753934157
2360 2360 c, t dbSNP:552859227
2369 2369 a, g dbSNP:757882877
2379 2379 c, g dbSNP:779595582
2384 2384 -, cgtt dbSNP:747996231
2385 2385 c, t dbSNP:746329878
2386 2386 a, g dbSNP:376422017
2389 2389 a, g dbSNP:780400544
2397 2397 c, t dbSNP:747820998
2398 2398 a, g dbSNP:766917705
2399 2399 a, g dbSNP:769474449
2404 2404 c, g dbSNP:772644384
2414 2414 c, g dbSNP:762417969
2430 2430 c, g dbSNP:770386103
2431 2431 a, g dbSNP:774290221
2440 2440 a, g dbSNP:759407054
2441 2441 a, c dbSNP:541669728
2442 2442 c, t dbSNP:767285617
2448 2448 c, g, t dbSNP:752528578
2451 2451 g, t dbSNP:764348919
2454 2454 a, c dbSNP:370910801
2479 2479 c, t dbSNP:138527651
2482 2482 a, t dbSNP:779079165
2487 2487 a, g dbSNP:750971301
2496 2496 a, g dbSNP:758968889
2500 2500 a, g dbSNP:121908074
2505 2505 c, t dbSNP:373662034
2506 2506 a, g dbSNP:144147585
2513 2513 a, t dbSNP:769370851
2528 2528 a, g dbSNP:397517837
2529 2529 c, t dbSNP:777510027
2532 2532 g, t dbSNP:727503487
2556 2556 a, g dbSNP:745353027
2563 2563 c, g dbSNP:540988111
2564 2564 a, g dbSNP:561207896
2567 2567 a, t dbSNP:764992976
2578 2578 a, g dbSNP:768503451
2584 2584 a, c, g dbSNP:199819595
2589 2589 c, t dbSNP:184318138
2590 2590 a, c, g dbSNP:563322370
2598 2598 a, c dbSNP:766317205
2602 2602 c, t dbSNP:752117955
2606 2606 c, t dbSNP:755369550
2608 2608 a, g dbSNP:397517838
2609 2609 c, t dbSNP:371947894
2610 2610 a, g dbSNP:145757452
2625 2625 g, t dbSNP:756948278
2634 2634 c, t dbSNP:778570792
2635 2635 a, c dbSNP:745477868
2642 2642 a, t dbSNP:757941226
2643 2643 a, g dbSNP:779366921
2644 2644 a, g dbSNP:747035869
2651 2651 c, g dbSNP:376843201
2660 2660 c, t dbSNP:768465472
2667 2667 a, g dbSNP:776617166
2673 2673 a, g dbSNP:535337741
2674 2674 g, t dbSNP:781309408
2679 2679 c, t dbSNP:748048935
2682 2682 g, t dbSNP:769722158
2684 2684 a, t dbSNP:41310067
2690 2690 a, g dbSNP:749588216
2707 2707 c, t dbSNP:771042716
2714 2714 -, cagcga dbSNP:776914236
2717 2717 c, t dbSNP:573874378
2718 2718 a, g dbSNP:555517698
2721 2721 a, t dbSNP:532762720
2732 2732 a, g dbSNP:775993082
2733 2733 a, g dbSNP:761105181
2736 2736 c, g dbSNP:142701058
2741 2741 a, c, g, t dbSNP:201945537
2754 2754 c, t dbSNP:111387206
2770 2770 a, c, t dbSNP:150738413
2771 2771 a, g dbSNP:756166626
2772 2772 a, c dbSNP:763972600
2777 2777 a, g dbSNP:753763142
2785 2785 g, t dbSNP:139102393
2786 2786 c, t dbSNP:778706687
2794 2794 c, t dbSNP:149506642
2795 2795 a, g dbSNP:191912512
2807 2807 c, t dbSNP:768121893
2813 2813 a, g dbSNP:776193466
2815 2815 c, t dbSNP:142073640
2816 2816 a, g, t dbSNP:765191961
2818 2818 a, c dbSNP:570411548
2823 2823 a, g dbSNP:766103436
2824 2824 a, t dbSNP:727503488
2832 2832 a, c dbSNP:751784785
2838 2838 a, g dbSNP:727503489
2849 2849 c, g dbSNP:191962062
2851 2851 a, c dbSNP:151157872
2854 2854 c, g dbSNP:752951470
2857 2857 c, t dbSNP:752796394
2861 2861 g, t dbSNP:200052749
2866 2866 a, g dbSNP:756617297
2867 2867 c, t dbSNP:778452071
2871 2871 a, g dbSNP:749757787
2874 2874 a, g dbSNP:771430357
2878 2878 a, c, t dbSNP:779419424
2879 2879 g, t dbSNP:768332156
2891 2891 c, t dbSNP:776047035
2892 2892 a, g dbSNP:761420487
2895 2895 c, t dbSNP:769243105
2899 2899 c, g dbSNP:773145642
2902 2902 c, g dbSNP:542333156
2905 2905 a, g dbSNP:766228750
2906 2906 c, t dbSNP:751338614
2907 2907 a, g dbSNP:759722630
2916 2916 -, ctg dbSNP:778918978
2916 2916 c, t dbSNP:184207177
2919 2919 c, t dbSNP:752820446
2926 2926 c, t dbSNP:756234468
2929 2929 c, g dbSNP:79830675
2933 2933 c, t dbSNP:754465672
2936 2936 c, g dbSNP:757829981
2938 2938 c, t dbSNP:779468901
2944 2944 a, g dbSNP:532663229
2950 2950 c, t dbSNP:746213740
2956 2956 c, t dbSNP:758652022
2957 2957 a, c, g dbSNP:780922035
2967 2967 a, g dbSNP:769296389
2979 2979 c, t dbSNP:545955592
2984 2984 -, ccta dbSNP:748666879
3006 3006 a, g dbSNP:71507808
3045 3045 c, t dbSNP:555045583
3048 3048 c, t dbSNP:111371259
3066 3066 a, g dbSNP:747341513
3070 3070 -, t dbSNP:71495343
3084 3084 -, tt dbSNP:78220845
3101 3101 a, g dbSNP:145061856
3144 3144 g, t dbSNP:4745199
3147 3147 a, g dbSNP:578244121
3154 3154 a, t dbSNP:189532970

Target ORF information:

RefSeq Version NM_138691
Organism Homo sapiens (human)
Definition Homo sapiens transmembrane channel-like 1 (TMC1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_138691

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTCACCCA AAAAAGTACA AATCAAAGTG GAGGAAAAAG AAGACGAGAC TGAGGAAAGC 
TCAAGTGAAG AGGAAGAGGA GGTGGAAGAT AAGCTACCTC GAAGAGAGAG CTTGAGACCA
AAGAGGAAAC GGACCAGAGA TGTTATCAAT GAGGATGACC CAGAACCTGA ACCAGAGGAT
GAAGAAACAA GGAAGGCAAG AGAAAAAGAG AGGAGGAGGA GGCTAAAGAG AGGAGCAGAA
GAAGAAGAAA TTGATGAAGA GGAATTGGAA AGATTGAAGG CAGAGTTAGA TGAGAAAAGA
CAAATAATTG CTACTGTCAA ATGCAAACCA TGGAAGATGG AGAAGAAAAT TGAAGTTCTC
AAGGAGGCAA AAAAATTTGT GAGTGAAAAT GAAGGGGCTC TTGGGAAAGG AAAAGGAAAA
CGGTGGTTTG CATTTAAGAT GATGATGGCC AAGAAATGGG CAAAATTCCT CCGTGATTTT
GAGAACTTCA AAGCTGCGTG TGTCCCATGG GAAAATAAAA TCAAGGCTAT TGAAAGTCAG
TTTGGCTCCT CAGTGGCCTC ATACTTCCTC TTCTTGAGAT GGATGTATGG AGTCAATATG
GTTCTCTTTA TCCTGACATT TAGCCTCATC ATGTTGCCAG AGTACCTCTG GGGTTTGCCA
TATGGCAGTT TACCTAGGAA AACCGTTCCC AGAGCCGAAG AGGCATCGGC AGCAAACTTT
GGTGTGTTGT ACGACTTCAA TGGTTTGGCA CAATATTCCG TTCTCTTTTA TGGCTATTAT
GACAATAAAC GAACAATTGG ATGGATGAAT TTCAGGTTGC CGCTCTCCTA TTTTCTAGTG
GGGATTATGT GCATTGGATA CAGCTTTCTG GTTGTCCTCA AAGCAATGAC CAAAAACATT
GGTGATGATG GAGGTGGAGA TGACAACACT TTCAATTTCA GCTGGAAGGT CTTTACCAGC
TGGGACTACC TGATCGGCAA TCCTGAAACA GCAGACAACA AATTTAATTC TATCACAATG
AACTTTAAGG AAGCTATCAC AGAAGAAAAA GCAGCCCAAG TAGAAGAAAA CGTCCACTTG
ATCAGATTCC TGAGGTTTCT GGCTAACTTC TTCGTGTTTC TAACACTTGG AGGGAGTGGA
TACCTCATCT TTTGGGCTGT GAAGCGATCC CAGGAATTTG CACAGCAAGA TCCTGACACC
CTTGGGTGGT GGGAAAAAAA TGAAATGAAC ATGGTTATGT CCCTCCTAGG GATGTTCTGT
CCAACATTGT TTGACTTATT TGCTGAATTA GAAGACTACC ATCCTCTCAT CGCTTTGAAA
TGGCTACTGG GACGCATTTT TGCTCTTCTT TTAGGCAATT TATACGTATT TATTCTTGCA
TTAATGGATG AGATTAACAA CAAGATTGAA GAGGAGAAGC TAGTAAAGGC CAATATTACC
CTTTGGGAAG CCAATATGAT CAAGGCCTAC AATGCATCAT TCTCTGAAAA TAGCACTGGA
CCACCCTTTT TTGTTCACCC TGCAGATGTA CCTCGAGGAC CTTGCTGGGA AACAATGGTG
GGACAGGAGT TTGTGAGGCT GACAGTCTCT GATGTTCTGA CCACCTACGT CACAATCCTC
ATTGGGGACT TTCTAAGGGC ATGTTTTGTG AGGTTTTGCA ATTATTGCTG GTGCTGGGAC
TTGGAGTATG GATATCCTTC ATACACCGAA TTCGACATCA GTGGCAACGT CCTCGCTCTG
ATCTTCAACC AAGGCATGAT CTGGATGGGC TCCTTCTTTG CTCCCAGCCT CCCAGGCATC
AATATCCTTC GACTCCATAC ATCCATGTAC TTCCAGTGCT GGGCCGTTAT GTGCTGCAAT
GTTCCTGAGG CCAGGGTCTT CAAAGCTTCC AGATCAAATA ACTTCTACCT GGGCATGCTA
CTGCTCATCC TCTTCCTGTC CACAATGCCT GTCTTGTACA TGATCGTGTC CCTCCCACCA
TCTTTTGATT GTGGTCCATT CAGTGGCAAA AATAGAATGT TTGAAGTCAT TGGAGAGACC
CTGGAGCACG ATTTCCCAAG CTGGATGGCG AAGATCTTGA GACAGCTTTC AAACCCTGGG
CTGGTCATTG CTGTCATTTT GGTGATGGTT TTGGCCATCT ATTATCTCAA TGCTACTGCC
AAGGGCCAGA AGGCAGCGAA TCTGGATCTC AAAAAGAAGA TGAAAATGCA AGCTTTGGAG
AACAAAATGC GAAACAAGAA AATGGCAGCT GCACGAGCAG CTGCAGCTGC TGGTCGCCAG
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu75047
Accession Version XM_011518213.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product transmembrane channel-like protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_008470.20) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##RefSeq-Attributes-START## ab initio :: 19% of CDS bases ##RefSeq-Attributes-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGCCCTTGC CAAGCAGGTC AGCGGGGCAC AGGGACTGCG CTCCTCATGA TAGTGCAGAT 
TGGCTCGGAA GTACTCAATT CTGTGGAGTC ACAGCGCCCT CTGACAGATT TCAGTTTCTG
GAAAAGGAAG GGCCCCCTTC AGGGCAGATG TGGAAGAAGA TGGAAGCGAT GAAAACCTTC
TTGCCAAAAT CTCCCAAAAG ACCTGGCCAG TGGAGCTTAC TGACTATACT CAAGCAACAA
AATGCACACT TGGAAATCCT GTGTCACGTG CCTATACTAG CTTTGTCGCT GATTCAGCAT
ATACACATCC TCCAGTGTGA CAGGATTGTG TATGGAAACA GTTTCATAAT AGATCTCTTG
GAATATGGTC GTTTGGTGTT AAGCATTCTA TCTGGCAAAA TGAAAAATAA GATGGTAGCA
GTTGTGGGAG CTTCTGTGGA ACAGATTCAG GTGGTACAGC TGCTCAAACT CATAACTCAC
CTGCAATGTG TTCTGAAAAT GTGTGGCTTA ACTGCATCTA CCCTAAAAGA ACAAGTTGCC
TGTGGGGAAG GAACTGTCCA CGTGGAGTGG TCTGGTGAAT GCTTAAGGAG CTGCAGAAGG
GAAGTACAAA TCAAAGTGGA GGAAAAAGAA GACGAGACTG AGGAAAGCTC AAGTGAAGAG
GAAGAGGAGG TGGAAGATAA GCTACCTCGA AGAGAGAGCT TGAGACCAAA GAGGAAACGG
ACCAGAGATG TTATCAATGA GGATGACCCA GAACCTGAAC CAGAGGATGA AGAAACAAGG
AAGGCAAGAG AAAAAGAGAG GAGGAGGAGG CTAAAGAGAG GAGCAGAAGA AGAAGAAATT
GATGAAGAGG AATTGGAAAG ATTGAAGGCA GAGTTAGATG AGAAAAGACA AATAATTGCT
ACTGTCAAAT GCAAACCATG GAAGATGGAG AAGAAAATTG AAGTTCTCAA GGAGGCAAAA
AAATTTGTGA GTGAAAATGA AGGGGCTCTT GGGAAAGGAA AAGGAAAACG GTGGTTTGCA
TTTAAGATGA TGATGGCCAA GAAATGGGCA AAATTCCTCC GTGATTTTGA GAACTTCAAA
GCTGCGTGTG TCCCATGGGA AAATAAAATC AAGGCTATTG AAAGTCAGTT TGGCTCCTCA
GTGGCCTCAT ACTTCCTCTT CTTGAGATGG ATGTATGGAG TCAATATGGT TCTCTTTATC
CTGACATTTA GCCTCATCAT GTTGCCAGAG TACCTCTGGG GTTTGCCATA TGGCAGTTTA
CCTAGGAAAA CCGTTCCCAG AGCCGAAGAG GCATCGGCAG CAAACTTTGG TGTGTTGTAC
GACTTCAATG GTTTGGCACA ATATTCCGTT CTCTTTTATG GCTATTATGA CAATAAACGA
ACAATTGGAT GGATGAATTT CAGGTTGCCG CTCTCCTATT TTCTAGTGGG GATTATGTGC
ATTGGATACA GCTTTCTGGT TGTCCTCAAA GCAATGACCA AAAACATTGG TGATGATGGA
GGTGGAGATG ACAACACTTT CAATTTCAGC TGGAAGGTCT TTACCAGCTG GGACTACCTG
ATCGGCAATC CTGAAACAGC AGACAACAAA TTTAATTCTA TCACAATGAA CTTTAAGGAA
GCTATCACAG AAGAAAAAGC AGCCCAAGTA GAAGAAAACG TCCACTTGAT CAGATTCCTG
AGGTTTCTGG CTAACTTCTT CGTGTTTCTA ACACTTGGAG GGAGTGGATA CCTCATCTTT
TGGGCTGTGA AGCGATCCCA GGAATTTGCA CAGCAAGATC CTGACACCCT TGGGTGGTGG
GAAAAAAATG AAATGAACAT GGTTATGTCC CTCCTAGGGA TGTTCTGTCC AACATTGTTT
GACTTATTTG CTGAATTAGA AGACTACCAT CCTCTCATCG CTTTGAAATG GCTACTGGGA
CGCATTTTTG CTCTTCTTTT AGGCAATTTA TACGTATTTA TTCTTGCATT AATGGATGAG
ATTAACAACA AGATTGAAGA GGAGAAGCTA GTAAAGGCCA ATATTACCCT TTGGGAAGCC
AATATGATCA AGGCCTACAA TGCATCATTC TCTGAAAATA GCACTGGACC ACCCTTTTTT
GTTCACCCTG CAGATGTACC TCGAGGACCT TGCTGGGAAA CAATGGTGGG ACAGGAGTTT
GTGAGGCTGA CAGTCTCTGA TGTTCTGACC ACCTACGTCA CAATCCTCAT TGGGGACTTT
CTAAGGGCAT GTTTTGTGAG GTTTTGCAAT TATTGCTGGT GCTGGGACTT GGAGTATGGA
TATCCTTCAT ACACCGAATT CGACATCAGT GGCAACGTCC TCGCTCTGAT CTTCAACCAA
GGCATGATCT GGATGGGCTC CTTCTTTGCT CCCAGCCTCC CAGGCATCAA TATCCTTCGA
CTCCATACAT CCATGTACTT CCAGTGCTGG GCCGTTATGT GCTGCAATGT TCCTGAGGCC
AGGGTCTTCA AAGCTTCCAG ATCAAATAAC TTCTACCTGG GCATGCTACT GCTCATCCTC
TTCCTGTCCA CAATGCCTGT CTTGTACATG ATCGTGTCCC TCCCACCATC TTTTGATTGT
GGTCCATTCA GTGGCAAAAA TAGAATGTTT GAAGTCATTG GAGAGACCCT GGAGCACGAT
TTCCCAAGCT GGATGGCGAA GATCTTGAGA CAGCTTTCAA ACCCTGGGCT GGTCATTGCT
GTCATTTTGG TGATGGTTTT GGCCATCTAT TATCTCAATG CTACTGCCAA GGGCCAGAAG
GCAGCGAATC TGGATCTCAA AAAGAAGATG AAAATGCAAG CTTTGGAGAA CAAAATGCGA
AACAAGAAAA TGGCAGCTGC ACGAGCAGCT GCAGCTGCTG GTCGCCAGTA A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011516515.1
CDS1..2871
Misc Feature(1)2131..2478(+)
Translation
Position Chain Variation Link
16 16 a, g dbSNP:2278669
37 37 c, t dbSNP:560402772
38 38 a, g dbSNP:118139255
40 40 c, g dbSNP:377366528
121 121 g, t dbSNP:746684049
149 149 c, t dbSNP:572232827
176 176 c, g dbSNP:541108280
199 199 a, g dbSNP:192185935
216 216 c, t dbSNP:547526351
263 263 a, g dbSNP:539268944
276 276 a, g dbSNP:769461506
287 287 c, t dbSNP:73651607
323 323 a, g dbSNP:529794682
333 333 c, t dbSNP:370176380
361 361 a, g dbSNP:558785930
370 370 c, t dbSNP:377423815
371 371 a, g dbSNP:534470454
378 378 a, g dbSNP:554333067
416 416 c, t dbSNP:776426312
446 446 g, t dbSNP:761687817
486 486 a, g dbSNP:115271477
488 488 c, g dbSNP:774744327
505 505 a, g dbSNP:560443545
541 541 g, t dbSNP:768959194
546 546 a, g dbSNP:750183854
549 549 a, g dbSNP:183039764
561 561 c, t dbSNP:776867976
562 562 a, g dbSNP:758655552
599 599 g, t dbSNP:575997087
608 608 -, a dbSNP:757999109
610 610 -, a dbSNP:727504554
611 611 a, t dbSNP:372383725
619 619 c, g dbSNP:757765718
632 632 a, t dbSNP:779209726
633 633 c, t dbSNP:2589615
634 634 a, g dbSNP:140437301
640 640 a, g dbSNP:776220877
647 647 c, g dbSNP:189479995
652 652 a, g, t dbSNP:144387828
666 666 a, g dbSNP:769476838
667 667 a, g dbSNP:777217789
672 672 a, g dbSNP:748771130
675 675 a, g dbSNP:770793343
677 677 a, g dbSNP:367993839
688 688 c, t dbSNP:121908073
689 689 a, g dbSNP:753977925
715 715 -, a dbSNP:777419888
718 718 c, t dbSNP:775411731
719 719 a, g dbSNP:572854741
723 723 a, c dbSNP:111353632
725 725 g, t dbSNP:753879286
729 729 a, g, t dbSNP:140388347
730 730 a, g dbSNP:562374634
733 733 a, c dbSNP:149947445
748 748 c, t dbSNP:780458439
765 765 a, g dbSNP:751950994
769 769 a, g dbSNP:755733171
781 781 a, g dbSNP:145098032
784 784 a, g dbSNP:551184500
791 791 a, g dbSNP:564668916
799 799 a, g dbSNP:779134204
807 807 -, ggagg dbSNP:730880359
815 815 a, g dbSNP:778543840
824 824 a, c, t dbSNP:140482278
825 825 -, aga dbSNP:376040866
826 826 a, g dbSNP:145647775
829 829 a, g dbSNP:1796993
831 831 a, c dbSNP:536350054
835 835 -, gaa dbSNP:397517839
881 881 a, g dbSNP:767739170
904 904 a, g dbSNP:753572224
921 921 a, g dbSNP:756957433
926 926 c, t dbSNP:778631855
927 927 a, g dbSNP:397517840
930 930 a, g dbSNP:370554600
938 938 c, t dbSNP:758421299
950 950 a, g dbSNP:368673178
954 954 a, g dbSNP:759933822
956 956 -, a dbSNP:769806797
957 957 -, a dbSNP:745767539
961 961 a, c dbSNP:377607548
964 964 a, t dbSNP:752998493
970 970 a, g dbSNP:372983741
972 972 c, t dbSNP:764922731
974 974 a, t dbSNP:749978207
991 991 a, g dbSNP:397517841
1002 1002 a, g dbSNP:566767136
1009 1009 a, c, t dbSNP:11143384
1028 1028 c, t dbSNP:781112060
1036 1036 a, g dbSNP:534560340
1048 1048 g, t dbSNP:145102585
1049 1049 c, t dbSNP:371485372
1050 1050 a, g dbSNP:539228804
1056 1056 a, c dbSNP:761235546
1060 1060 c, t dbSNP:200841002
1061 1061 a, g dbSNP:148340276
1071 1071 a, g dbSNP:375984349
1081 1081 c, g dbSNP:762736726
1085 1085 c, t dbSNP:765885670
1086 1086 a, g dbSNP:370032879
1092 1092 c, g dbSNP:758924923
1095 1095 a, g dbSNP:767019298
1097 1097 a, g dbSNP:752678337
1117 1117 a, g dbSNP:756028904
1118 1118 c, t dbSNP:747645067
1120 1120 -, a dbSNP:762864983
1124 1124 a, g dbSNP:770640925
1132 1132 a, g dbSNP:773838098
1133 1133 a, g dbSNP:199560971
1137 1137 c, t dbSNP:375128682
1144 1144 a, g dbSNP:774972622
1145 1145 c, g, t dbSNP:727504470
1173 1173 g, t dbSNP:368246679
1177 1177 a, g dbSNP:753687760
1184 1184 a, g, t dbSNP:756960425
1187 1187 c, t dbSNP:750662616
1191 1191 g, t dbSNP:372449375
1193 1193 c, t dbSNP:780348810
1194 1194 c, g dbSNP:376768305
1206 1206 a, c dbSNP:199499836
1207 1207 a, t dbSNP:368042336
1212 1212 a, c dbSNP:781747541
1215 1215 c, t dbSNP:748580616
1216 1216 a, g dbSNP:565926145
1220 1220 g, t dbSNP:773609760
1225 1225 c, t dbSNP:745569653
1239 1239 g, t dbSNP:150896910
1244 1244 c, t dbSNP:749608265
1246 1246 a, c dbSNP:771735027
1249 1249 c, t dbSNP:779639998
1250 1250 a, g dbSNP:533159785
1262 1262 c, t dbSNP:370088722
1266 1266 c, g dbSNP:776193666
1273 1273 a, g dbSNP:201827013
1284 1284 c, t dbSNP:112843964
1285 1285 a, g dbSNP:373537563
1290 1290 a, g dbSNP:762790929
1291 1291 g, t dbSNP:200831684
1293 1293 a, g dbSNP:560655583
1295 1295 c, t dbSNP:751873680
1296 1296 a, g dbSNP:759815339
1304 1304 a, g dbSNP:767557406
1310 1310 a, g dbSNP:752751599
1323 1323 c, t dbSNP:143404572
1326 1326 c, g dbSNP:778293772
1328 1328 a, g dbSNP:549050367
1333 1333 a, t dbSNP:750724247
1347 1347 c, t dbSNP:143200611
1348 1348 a, g dbSNP:111033497
1349 1349 c, t dbSNP:375919123
1353 1353 c, t dbSNP:201230747
1363 1363 c, t dbSNP:764050135
1365 1365 c, t dbSNP:727503482
1368 1368 c, t dbSNP:754390601
1374 1374 c, t dbSNP:780868794
1378 1378 c, t dbSNP:765657443
1379 1379 a, c, g dbSNP:147147941
1383 1383 a, c dbSNP:140398130
1384 1384 a, g dbSNP:536753857
1385 1385 c, t dbSNP:747645756
1391 1391 g, t dbSNP:750373958
1402 1402 a, g dbSNP:111839361
1409 1409 c, t dbSNP:755694066
1410 1410 a, g dbSNP:370523728
1413 1413 c, t dbSNP:749302129
1415 1415 a, c dbSNP:770921263
1418 1418 -, t dbSNP:752999399
1418 1418 a, g dbSNP:774299359
1428 1428 -, g dbSNP:758763154
1429 1429 g, t dbSNP:397517842
1438 1438 c, t dbSNP:745607141
1467 1467 c, g, t dbSNP:772213890
1472 1472 c, g, t dbSNP:760785189
1477 1477 a, t dbSNP:776707718
1481 1481 a, g dbSNP:149912546
1489 1489 a, g dbSNP:770320624
1490 1490 c, g dbSNP:773751182
1494 1494 g, t dbSNP:763351588
1495 1495 g, t dbSNP:766828978
1511 1511 -, aca dbSNP:778174231
1512 1512 c, g, t dbSNP:113342704
1516 1516 a, g dbSNP:144501871
1523 1523 a, g dbSNP:753426697
1524 1524 g, t dbSNP:148443938
1544 1544 c, g dbSNP:142629075
1545 1545 c, g, t dbSNP:750432713
1548 1548 -, c dbSNP:751195625
1552 1552 g, t dbSNP:779905040
1556 1556 a, g dbSNP:746724027
1568 1568 a, t dbSNP:768891360
1572 1572 c, t dbSNP:781353378
1575 1575 a, c dbSNP:748342146
1583 1583 a, g dbSNP:769913872
1590 1590 a, g dbSNP:773156746
1598 1598 a, c dbSNP:376305043
1606 1606 a, g dbSNP:771281791
1607 1607 c, t dbSNP:111033464
1610 1610 a, c dbSNP:727504568
1627 1627 a, g dbSNP:749274559
1639 1639 a, g dbSNP:771497286
1658 1658 a, g dbSNP:397517834
1660 1660 a, c, g dbSNP:774756982
1666 1666 a, t dbSNP:772620520
1672 1672 a, g dbSNP:775992461
1673 1673 a, g dbSNP:761560850
1678 1678 c, t dbSNP:764926506
1688 1688 g, t dbSNP:768955462
1699 1699 c, t dbSNP:758739636
1701 1701 c, t dbSNP:762332645
1702 1702 a, g dbSNP:367924428
1710 1710 a, g dbSNP:751503196
1717 1717 a, g dbSNP:754899734
1718 1718 a, g dbSNP:767369935
1720 1720 a, g dbSNP:752427037
1727 1727 a, g dbSNP:372215112
1728 1728 a, c dbSNP:777814216
1729 1729 a, t dbSNP:749491943
1731 1731 a, c dbSNP:757327146
1734 1734 c, t dbSNP:779035669
1753 1753 c, t dbSNP:151001642
1754 1754 a, g dbSNP:772640673
1760 1760 a, c dbSNP:776010914
1768 1768 g, t dbSNP:374313036
1770 1770 a, g dbSNP:769519448
1773 1773 a, g dbSNP:371815403
1777 1777 a, g dbSNP:185702148
1787 1787 c, g dbSNP:765865619
1797 1797 c, g dbSNP:773851192
1800 1800 c, g dbSNP:759478413
1803 1803 a, c dbSNP:767390037
1809 1809 -, t dbSNP:769571760
1821 1821 a, g dbSNP:184603559
1822 1822 c, g dbSNP:568058201
1830 1830 c, t dbSNP:371854262
1839 1839 a, g dbSNP:771536758
1840 1840 a, g dbSNP:775453038
1841 1841 a, t dbSNP:786201027
1844 1844 c, t dbSNP:760645934
1851 1851 a, g dbSNP:139985214
1855 1855 c, t dbSNP:753600914
1856 1856 g, t dbSNP:761954491
1863 1863 c, t dbSNP:765507648
1867 1867 c, t dbSNP:750563026
1872 1872 c, t dbSNP:758592011
1873 1873 a, g dbSNP:780260906
1881 1881 a, c dbSNP:752172170
1889 1889 a, c dbSNP:375264737
1890 1890 a, c, t dbSNP:781418962
1897 1897 a, c dbSNP:770517350
1899 1899 c, t dbSNP:537381495
1900 1900 a, g dbSNP:745495139
1917 1917 a, g dbSNP:369197132
1919 1919 a, g dbSNP:771625117
1921 1921 c, t dbSNP:372710475
1922 1922 a, g dbSNP:760532554
1936 1936 c, t dbSNP:768579412
1939 1939 g, t dbSNP:776311501
1947 1947 c, t dbSNP:761629265
1950 1950 a, g dbSNP:765094337
1953 1953 c, t dbSNP:750724544
1954 1954 a, g dbSNP:763255951
1957 1957 c, t dbSNP:397517836
1963 1963 c, t dbSNP:751594947
1973 1973 c, t dbSNP:755457390
1975 1975 a, g dbSNP:758352261
1984 1984 -, aac dbSNP:780156287
1991 1991 a, g dbSNP:753108960
2007 2007 a, c, g dbSNP:371004715
2008 2008 c, t dbSNP:756605797
2016 2016 a, g dbSNP:373847743
2032 2032 c, t dbSNP:754142954
2042 2042 a, g dbSNP:758068479
2045 2045 c, t dbSNP:17058153
2048 2048 g, t dbSNP:746611422
2054 2054 c, t dbSNP:368087393
2063 2063 c, t dbSNP:780555614
2065 2065 a, t dbSNP:748118938
2066 2066 c, t dbSNP:769847194
2073 2073 g, t dbSNP:370302201
2086 2086 a, g dbSNP:773195404
2087 2087 a, g dbSNP:749066542
2090 2090 c, t dbSNP:771313896
2092 2092 c, t dbSNP:774660132
2120 2120 a, c dbSNP:727503483
2122 2122 c, t dbSNP:200171616
2131 2131 c, t dbSNP:121908076
2132 2132 g, t dbSNP:767642609
2150 2150 g, t dbSNP:146558576
2151 2151 a, g dbSNP:761230185
2154 2154 a, g dbSNP:372344935
2158 2158 c, t dbSNP:777145557
2172 2172 a, c dbSNP:762248733
2174 2174 a, t dbSNP:761621035
2176 2176 c, t dbSNP:750746559
2182 2182 a, g dbSNP:759289124
2190 2190 c, g dbSNP:111033462
2196 2196 c, t dbSNP:552170649
2197 2197 a, g dbSNP:150206751
2199 2199 c, t dbSNP:777364081
2208 2208 c, t dbSNP:145772877
2210 2210 c, t dbSNP:201955337
2214 2214 a, g dbSNP:778829243
2217 2217 c, t dbSNP:745630603
2220 2220 c, t dbSNP:772378812
2236 2236 g, t dbSNP:201465054
2240 2240 a, g dbSNP:747186037
2246 2246 g, t dbSNP:768736139
2248 2248 a, t dbSNP:776624164
2266 2266 a, g dbSNP:762371411
2267 2267 a, t dbSNP:770186250
2268 2268 c, t dbSNP:773672736
2280 2280 a, t dbSNP:763192082
2293 2293 a, g dbSNP:148971770
2295 2295 c, g dbSNP:760464772
2296 2296 a, g dbSNP:369890353
2301 2301 c, t dbSNP:34532421
2302 2302 a, c, g dbSNP:121908072
2316 2316 c, t dbSNP:761261855
2322 2322 c, t dbSNP:374323593
2330 2330 g, t dbSNP:764756492
2331 2331 a, c dbSNP:750341293
2338 2338 a, c dbSNP:758288878
2339 2339 a, g dbSNP:377731576
2350 2350 c, t dbSNP:766228632
2352 2352 a, g dbSNP:368084452
2356 2356 a, g dbSNP:759355645
2361 2361 c, t dbSNP:767247084
2373 2373 c, t dbSNP:752871843
2382 2382 a, g dbSNP:756359010
2384 2384 a, g dbSNP:727503486
2386 2386 a, g dbSNP:202144745
2398 2398 c, t dbSNP:777777359
2402 2402 c, t dbSNP:753934157
2408 2408 c, t dbSNP:552859227
2417 2417 a, g dbSNP:757882877
2427 2427 c, g dbSNP:779595582
2432 2432 -, cgtt dbSNP:747996231
2433 2433 c, t dbSNP:746329878
2434 2434 a, g dbSNP:376422017
2437 2437 a, g dbSNP:780400544
2445 2445 c, t dbSNP:747820998
2446 2446 a, g dbSNP:766917705
2447 2447 a, g dbSNP:769474449
2452 2452 c, g dbSNP:772644384
2462 2462 c, g dbSNP:762417969
2478 2478 c, g dbSNP:770386103
2479 2479 a, g dbSNP:774290221
2488 2488 a, g dbSNP:759407054
2489 2489 a, c dbSNP:541669728
2490 2490 c, t dbSNP:767285617
2496 2496 c, g, t dbSNP:752528578
2499 2499 g, t dbSNP:764348919
2502 2502 a, c dbSNP:370910801
2527 2527 c, t dbSNP:138527651
2530 2530 a, t dbSNP:779079165
2535 2535 a, g dbSNP:750971301
2544 2544 a, g dbSNP:758968889
2548 2548 a, g dbSNP:121908074
2553 2553 c, t dbSNP:373662034
2554 2554 a, g dbSNP:144147585
2561 2561 a, t dbSNP:769370851
2576 2576 a, g dbSNP:397517837
2577 2577 c, t dbSNP:777510027
2580 2580 g, t dbSNP:727503487
2604 2604 a, g dbSNP:745353027
2611 2611 c, g dbSNP:540988111
2612 2612 a, g dbSNP:561207896
2615 2615 a, t dbSNP:764992976
2626 2626 a, g dbSNP:768503451
2632 2632 a, c, g dbSNP:199819595
2637 2637 c, t dbSNP:184318138
2638 2638 a, c, g dbSNP:563322370
2646 2646 a, c dbSNP:766317205
2650 2650 c, t dbSNP:752117955
2654 2654 c, t dbSNP:755369550
2656 2656 a, g dbSNP:397517838
2657 2657 c, t dbSNP:371947894
2658 2658 a, g dbSNP:145757452
2673 2673 g, t dbSNP:756948278
2682 2682 c, t dbSNP:778570792
2683 2683 a, c dbSNP:745477868
2690 2690 a, t dbSNP:757941226
2691 2691 a, g dbSNP:779366921
2692 2692 a, g dbSNP:747035869
2699 2699 c, g dbSNP:376843201
2708 2708 c, t dbSNP:768465472
2715 2715 a, g dbSNP:776617166
2721 2721 a, g dbSNP:535337741
2722 2722 g, t dbSNP:781309408
2727 2727 c, t dbSNP:748048935
2730 2730 g, t dbSNP:769722158
2732 2732 a, t dbSNP:41310067
2738 2738 a, g dbSNP:749588216
2755 2755 c, t dbSNP:771042716
2762 2762 -, cagcga dbSNP:776914236
2765 2765 c, t dbSNP:573874378
2766 2766 a, g dbSNP:555517698
2769 2769 a, t dbSNP:532762720
2780 2780 a, g dbSNP:775993082
2781 2781 a, g dbSNP:761105181
2784 2784 c, g dbSNP:142701058
2789 2789 a, c, g, t dbSNP:201945537
2802 2802 c, t dbSNP:111387206
2818 2818 a, c, t dbSNP:150738413
2819 2819 a, g dbSNP:756166626
2820 2820 a, c dbSNP:763972600
2825 2825 a, g dbSNP:753763142
2833 2833 g, t dbSNP:139102393
2834 2834 c, t dbSNP:778706687
2842 2842 c, t dbSNP:149506642
2843 2843 a, g dbSNP:191912512
2855 2855 c, t dbSNP:768121893
2861 2861 a, g dbSNP:776193466
2863 2863 c, t dbSNP:142073640
2864 2864 a, g, t dbSNP:765191961
2866 2866 a, c dbSNP:570411548
2871 2871 a, g dbSNP:766103436
2872 2872 a, t dbSNP:727503488
2880 2880 a, c dbSNP:751784785
2886 2886 a, g dbSNP:727503489
2897 2897 c, g dbSNP:191962062
2899 2899 a, c dbSNP:151157872
2902 2902 c, g dbSNP:752951470
2905 2905 c, t dbSNP:752796394
2909 2909 g, t dbSNP:200052749
2914 2914 a, g dbSNP:756617297
2915 2915 c, t dbSNP:778452071
2919 2919 a, g dbSNP:749757787
2922 2922 a, g dbSNP:771430357
2926 2926 a, c, t dbSNP:779419424
2927 2927 g, t dbSNP:768332156
2939 2939 c, t dbSNP:776047035
2940 2940 a, g dbSNP:761420487
2943 2943 c, t dbSNP:769243105
2947 2947 c, g dbSNP:773145642
2950 2950 c, g dbSNP:542333156
2953 2953 a, g dbSNP:766228750
2954 2954 c, t dbSNP:751338614
2955 2955 a, g dbSNP:759722630
2964 2964 -, ctg dbSNP:778918978
2964 2964 c, t dbSNP:184207177
2967 2967 c, t dbSNP:752820446
2974 2974 c, t dbSNP:756234468
2977 2977 c, g dbSNP:79830675
2981 2981 c, t dbSNP:754465672
2984 2984 c, g dbSNP:757829981
2986 2986 c, t dbSNP:779468901
2992 2992 a, g dbSNP:532663229
2998 2998 c, t dbSNP:746213740
3004 3004 c, t dbSNP:758652022
3005 3005 a, c, g dbSNP:780922035
3015 3015 a, g dbSNP:769296389
3027 3027 c, t dbSNP:545955592
3032 3032 -, ccta dbSNP:748666879
3054 3054 a, g dbSNP:71507808
3093 3093 c, t dbSNP:555045583
3096 3096 c, t dbSNP:111371259
3114 3114 a, g dbSNP:747341513
3118 3118 -, t dbSNP:71495343
3132 3132 -, tt dbSNP:78220845
3149 3149 a, g dbSNP:145061856
3192 3192 g, t dbSNP:4745199
3195 3195 a, g dbSNP:578244121
3202 3202 a, t dbSNP:189532970

Target ORF information:

RefSeq Version XM_011518213
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens transmembrane channel-like 1 (TMC1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011518213

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGCCCTTGC CAAGCAGGTC AGCGGGGCAC AGGGACTGCG CTCCTCATGA TAGTGCAGAT 
TGGCTCGGAA GTACTCAATT CTGTGGAGTC ACAGCGCCCT CTGACAGATT TCAGTTTCTG
GAAAAGGAAG GGCCCCCTTC AGGGCAGATG TGGAAGAAGA TGGAAGCGAT GAAAACCTTC
TTGCCAAAAT CTCCCAAAAG ACCTGGCCAG TGGAGCTTAC TGACTATACT CAAGCAACAA
AATGCACACT TGGAAATCCT GTGTCACGTG CCTATACTAG CTTTGTCGCT GATTCAGCAT
ATACACATCC TCCAGTGTGA CAGGATTGTG TATGGAAACA GTTTCATAAT AGATCTCTTG
GAATATGGTC GTTTGGTGTT AAGCATTCTA TCTGGCAAAA TGAAAAATAA GATGGTAGCA
GTTGTGGGAG CTTCTGTGGA ACAGATTCAG GTGGTACAGC TGCTCAAACT CATAACTCAC
CTGCAATGTG TTCTGAAAAT GTGTGGCTTA ACTGCATCTA CCCTAAAAGA ACAAGTTGCC
TGTGGGGAAG GAACTGTCCA CGTGGAGTGG TCTGGTGAAT GCTTAAGGAG CTGCAGAAGG
GAAGTACAAA TCAAAGTGGA GGAAAAAGAA GACGAGACTG AGGAAAGCTC AAGTGAAGAG
GAAGAGGAGG TGGAAGATAA GCTACCTCGA AGAGAGAGCT TGAGACCAAA GAGGAAACGG
ACCAGAGATG TTATCAATGA GGATGACCCA GAACCTGAAC CAGAGGATGA AGAAACAAGG
AAGGCAAGAG AAAAAGAGAG GAGGAGGAGG CTAAAGAGAG GAGCAGAAGA AGAAGAAATT
GATGAAGAGG AATTGGAAAG ATTGAAGGCA GAGTTAGATG AGAAAAGACA AATAATTGCT
ACTGTCAAAT GCAAACCATG GAAGATGGAG AAGAAAATTG AAGTTCTCAA GGAGGCAAAA
AAATTTGTGA GTGAAAATGA AGGGGCTCTT GGGAAAGGAA AAGGAAAACG GTGGTTTGCA
TTTAAGATGA TGATGGCCAA GAAATGGGCA AAATTCCTCC GTGATTTTGA GAACTTCAAA
GCTGCGTGTG TCCCATGGGA AAATAAAATC AAGGCTATTG AAAGTCAGTT TGGCTCCTCA
GTGGCCTCAT ACTTCCTCTT CTTGAGATGG ATGTATGGAG TCAATATGGT TCTCTTTATC
CTGACATTTA GCCTCATCAT GTTGCCAGAG TACCTCTGGG GTTTGCCATA TGGCAGTTTA
CCTAGGAAAA CCGTTCCCAG AGCCGAAGAG GCATCGGCAG CAAACTTTGG TGTGTTGTAC
GACTTCAATG GTTTGGCACA ATATTCCGTT CTCTTTTATG GCTATTATGA CAATAAACGA
ACAATTGGAT GGATGAATTT CAGGTTGCCG CTCTCCTATT TTCTAGTGGG GATTATGTGC
ATTGGATACA GCTTTCTGGT TGTCCTCAAA GCAATGACCA AAAACATTGG TGATGATGGA
GGTGGAGATG ACAACACTTT CAATTTCAGC TGGAAGGTCT TTACCAGCTG GGACTACCTG
ATCGGCAATC CTGAAACAGC AGACAACAAA TTTAATTCTA TCACAATGAA CTTTAAGGAA
GCTATCACAG AAGAAAAAGC AGCCCAAGTA GAAGAAAACG TCCACTTGAT CAGATTCCTG
AGGTTTCTGG CTAACTTCTT CGTGTTTCTA ACACTTGGAG GGAGTGGATA CCTCATCTTT
TGGGCTGTGA AGCGATCCCA GGAATTTGCA CAGCAAGATC CTGACACCCT TGGGTGGTGG
GAAAAAAATG AAATGAACAT GGTTATGTCC CTCCTAGGGA TGTTCTGTCC AACATTGTTT
GACTTATTTG CTGAATTAGA AGACTACCAT CCTCTCATCG CTTTGAAATG GCTACTGGGA
CGCATTTTTG CTCTTCTTTT AGGCAATTTA TACGTATTTA TTCTTGCATT AATGGATGAG
ATTAACAACA AGATTGAAGA GGAGAAGCTA GTAAAGGCCA ATATTACCCT TTGGGAAGCC
AATATGATCA AGGCCTACAA TGCATCATTC TCTGAAAATA GCACTGGACC ACCCTTTTTT
GTTCACCCTG CAGATGTACC TCGAGGACCT TGCTGGGAAA CAATGGTGGG ACAGGAGTTT
GTGAGGCTGA CAGTCTCTGA TGTTCTGACC ACCTACGTCA CAATCCTCAT TGGGGACTTT
CTAAGGGCAT GTTTTGTGAG GTTTTGCAAT TATTGCTGGT GCTGGGACTT GGAGTATGGA
TATCCTTCAT ACACCGAATT CGACATCAGT GGCAACGTCC TCGCTCTGAT CTTCAACCAA
GGCATGATCT GGATGGGCTC CTTCTTTGCT CCCAGCCTCC CAGGCATCAA TATCCTTCGA
CTCCATACAT CCATGTACTT CCAGTGCTGG GCCGTTATGT GCTGCAATGT TCCTGAGGCC
AGGGTCTTCA AAGCTTCCAG ATCAAATAAC TTCTACCTGG GCATGCTACT GCTCATCCTC
TTCCTGTCCA CAATGCCTGT CTTGTACATG ATCGTGTCCC TCCCACCATC TTTTGATTGT
GGTCCATTCA GTGGCAAAAA TAGAATGTTT GAAGTCATTG GAGAGACCCT GGAGCACGAT
TTCCCAAGCT GGATGGCGAA GATCTTGAGA CAGCTTTCAA ACCCTGGGCT GGTCATTGCT
GTCATTTTGG TGATGGTTTT GGCCATCTAT TATCTCAATG CTACTGCCAA GGGCCAGAAG
GCAGCGAATC TGGATCTCAA AAAGAAGATG AAAATGCAAG CTTTGGAGAA CAAAATGCGA
AACAAGAAAA TGGCAGCTGC ACGAGCAGCT GCAGCTGCTG GTCGCCAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.