Email to GenScript

CLCN5 chloride channel, voltage-sensitive 5 [Homo sapiens (human)]

Gene Symbol CLCN5
Entrez Gene ID 1184
Full Name chloride channel, voltage-sensitive 5
Synonyms CLC5, CLCK2, ClC-5, DENTS, NPHL1, NPHL2, XLRH, XRN, hCIC-K2
General protein information
Preferred Names
H(+)/Cl(-) exchange transporter 5
Names
H(+)/Cl(-) exchange transporter 5
chloride transporter ClC-5
voltage-gated chloride ion channel CLCN5
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp11.23-p11.22

Summary This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]. lac of sum
Disorder MIM:

300008

Disorder Html: Dent disease, 300009 (3); Nephrolithiasis, type I, 310468 (3);





The following CLCN5 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLCN5 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20940 NM_001282163 Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu56926 XM_011543888 PREDICTED: Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu56927 XM_011543889 PREDICTED: Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu21666 NM_001127899 Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu21666 NM_001127898 Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 Quote Price
OHu21358 NM_000084 Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $379
OHu16814 NM_001272102 Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu20940
Accession Version NM_001282163.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 05-MAY-2014
Organism Homo sapiens (human)
Product H(+)/Cl(-) exchange transporter 5 isoform d
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X91906.1, AC231645.2, BC051691.1, AK025562.1 and AA743724.1. On Aug 21, 2013 this sequence version replaced gi:442535526. Summary: This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]. Transcript Variant: This variant (4, also known as type 4) lacks several 5' exons but has an alternate 5' exon, and it thus differs in the 5' UTR and uses an alternate start codon, compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X91906.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCTAC CAGAGGACAA GTCGTACAAT GGTGGAGGAA TAGGTTCTTC AAATAGGATC 
ATGGACTTCT TGGAGGAGCC AATCCCTGGT GTAGGGACCT ATGATGATTT CAATACAATT
GATTGGGTGA GAGAGAAGTC TCGAGACCGG GATAGGCACC GAGAGATTAC CAATAAAAGC
AAAGAGTCAA CATGGGCCTT AATTCACAGT GTGAGTGATG CTTTTTCCGG CTGGTTGTTG
ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG TTAGCTGGTT TGATAGACAT CTCTGCTCAT
TGGATGACAG ACTTAAAAGA AGGTATATGC ACAGGGGGAT TCTGGTTTAA CCATGAACAT
TGTTGCTGGA ACTCTGAGCA TGTCACCTTT GAAGAGAGAG ACAAATGTCC AGAGTGGAAT
AGTTGGTCCC AGCTTATCAT CAGCACAGAT GAGGGAGCCT TTGCCTACAT AGTCAATTAT
TTCATGTACG TCCTCTGGGC TCTCCTATTT GCCTTCCTTG CCGTATCTCT TGTCAAGGTG
TTTGCGCCTT ATGCCTGTGG CTCTGGAATC CCTGAGATAA AAACTATCTT GAGTGGTTTC
ATTATTAGGG GCTATTTGGG TAAGTGGACT CTGGTTATCA AAACCATCAC CTTGGTGCTG
GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA GAGGGCCCTC TAGTGCACGT GGCTTGCTGC
TGTGGGAACA TCCTGTGCCA CTGCTTCAAC AAATACAGGA AGAATGAAGC CAAGCGCAGA
GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT GTATCTGTAG CCTTTGGAGC ACCTATAGGT
GGAGTATTAT TCAGCCTTGA AGAGGTCAGC TACTATTTTC CCCTCAAAAC ATTGTGGCGT
TCATTCTTTG CTGCCTTGGT GGCAGCATTC ACTCTACGCT CCATCAATCC ATTTGGGAAC
AGCCGCCTGG TACTATTTTA TGTGGAGTTT CACACCCCAT GGCATCTCTT TGAGCTCGTG
CCATTCATTC TGCTGGGCAT ATTTGGTGGT CTGTGGGGAG CACTGTTTAT CCGCACAAAC
ATTGCCTGGT GTCGGAAGCG AAAGACCACC CAGTTGGGCA AGTATCCTGT TATAGAGGTA
CTCGTCGTGA CAGCCATCAC TGCCATCCTG GCTTTCCCCA ATGAATACAC TCGGATGAGC
ACAAGTGAGC TCATTTCTGA GCTGTTTAAT GACTGTGGCC TTCTGGACTC CTCCAAGCTC
TGTGATTATG AGAACCGTTT CAACACAAGC AAAGGGGGTG AACTGCCTGA CAGACCGGCT
GGCGTGGGAG TCTACAGTGC AATGTGGCAG CTGGCTTTAA CACTCATACT GAAAATTGTC
ATTACTATAT TCACCTTTGG CATGAAGATC CCTTCTGGCC TCTTTATCCC TAGCATGGCT
GTTGGTGCTA TAGCAGGTCG ACTTCTAGGA GTAGGAATGG AACAGCTGGC TTATTACCAC
CAGGAATGGA CCGTCTTCAA TAGCTGGTGT AGTCAGGGAG CTGATTGCAT CACCCCCGGC
CTTTATGCAA TGGTTGGGGC TGCAGCCTGC TTAGGTGGGG TGACTCGGAT GACTGTTTCT
CTTGTTGTCA TAATGTTTGA ACTGACTGGT GGCTTAGAAT ACATCGTGCC TCTGATGGCT
GCAGCCATGA CAAGCAAGTG GGTGGCAGAT GCTCTTGGGC GGGAGGGCAT CTATGATGCC
CACATCCGTC TCAATGGATA CCCCTTTCTT GAAGCCAAAG AAGAGTTTGC TCATAAGACC
CTGGCAATGG ATGTGATGAA ACCCCGGAGA AATGATCCTT TGTTGACTGT CCTTACTCAG
GACAGTATGA CTGTGGAAGA TGTAGAGACC ATAATCAGTG AAACCACTTA CAGTGGCTTC
CCAGTGGTGG TATCCCGGGA GTCCCAAAGA CTTGTGGGCT TTGTCCTCCG AAGAGATCTC
ATTATTTCAA TTGAAAATGC TCGAAAGAAA CAGGATGGGG TTGTTAGCAC TTCCATCATT
TATTTCACGG AGCATTCTCC TCCATTGCCA CCATACACTC CACCCACTCT AAAGCTTCGG
AACATCCTCG ATCTCAGCCC CTTCACTGTG ACTGACCTTA CACCCATGGA GATCGTAGTG
GATATTTTCC GAAAGCTGGG ACTGCGGCAG TGCCTGGTTA CACACAACGG GCGATTGCTT
GGAATCATTA CCAAAAAGGA TGTGTTAAAG CATATAGCAC AGATGGCGAA CCAAGATCCT
GATTCCATTC TCTTCAACTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001269092.1
CDS232..2532
Misc Feature(1)226..228(+)
Misc Feature(2)505..2001(+)
Misc Feature(3)790..1965(+)
Misc Feature(4)922..924(+)
Misc Feature(5)1093..1095(+)
Misc Feature(6)2038..2478(+)
Misc Feature(7)2074..2484(+)
Exon (1)1..244
Gene:CLCN5
Gene Synonym:
Exon (2)245..396
Gene:CLCN5
Gene Synonym:
Exon (3)397..496
Gene:CLCN5
Gene Synonym:
Exon (4)497..684
Gene:CLCN5
Gene Synonym:
Exon (5)685..807
Gene:CLCN5
Gene Synonym:
Exon (6)808..1014
Gene:CLCN5
Gene Synonym:
Exon (7)1015..1095
Gene:CLCN5
Gene Synonym:
Exon (8)1096..1638
Gene:CLCN5
Gene Synonym:
Exon (9)1639..1825
Gene:CLCN5
Gene Synonym:
Exon (10)1826..2224
Gene:CLCN5
Gene Synonym:
Exon (11)2225..2441
Gene:CLCN5
Gene Synonym:
Exon (12)2442..9548
Gene:CLCN5
Gene Synonym:
Translation
Position Chain Variation Link
127 127 c, g dbSNP:112064121
461 461 g, t dbSNP:151340629
498 498 c, t dbSNP:113780806
635 635 a, g dbSNP:55676763
890 890 g, t dbSNP:151340622
948 948 c, t dbSNP:387907402
965 965 c, t dbSNP:273585645
1022 1022 c, t dbSNP:151340626
1070 1070 g, t dbSNP:151340630
1106 1106 a, g dbSNP:273585644
1125 1125 c, g dbSNP:273585648
1128 1128 a, g dbSNP:151340620
1130 1130 a, c, g dbSNP:151340628
1311 1311 a, c dbSNP:273585646
1319 1319 a, g dbSNP:151340627
1808 1808 a, g dbSNP:151340625
1828 1828 a, g dbSNP:273585647
1849 1849 c, t dbSNP:151340623
1909 1909 a, g dbSNP:387907403
1928 1928 a, g dbSNP:273585649
1930 1930 g, t dbSNP:273585650
2065 2065 a, g dbSNP:387907404
2233 2233 a, c, t dbSNP:151340621
2401 2401 c, t dbSNP:151340624
2881 2881 a, g dbSNP:111438914
3982 3982 c, t dbSNP:12835125
4132 4132 a, g dbSNP:12859644
4983 4983 a, c dbSNP:12687955
4992 4992 a, g dbSNP:111452206
5081 5081 c, t dbSNP:111369735
5512 5512 a, g dbSNP:56147923
5787 5787 c, t dbSNP:112868450
5941 5941 a, g dbSNP:59126550
6184 6184 c, t dbSNP:56657044
7363 7363 g, t dbSNP:12845771
7365 7365 a, g dbSNP:12837391
7676 7676 c, t dbSNP:111560086
8695 8695 c, t dbSNP:41312128

Target ORF information:

RefSeq Version NM_001282163
Organism Homo sapiens (human)
Definition Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282163

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGTTCCTAC CAGAGGACAA GTCGTACAAT GGTGGAGGAA TAGGTTCTTC AAATAGGATC 
ATGGACTTCT TGGAGGAGCC AATCCCTGGT GTAGGGACCT ATGATGATTT CAATACAATT
GATTGGGTGA GAGAGAAGTC TCGAGACCGG GATAGGCACC GAGAGATTAC CAATAAAAGC
AAAGAGTCAA CATGGGCCTT AATTCACAGT GTGAGTGATG CTTTTTCCGG CTGGTTGTTG
ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG TTAGCTGGTT TGATAGACAT CTCTGCTCAT
TGGATGACAG ACTTAAAAGA AGGTATATGC ACAGGGGGAT TCTGGTTTAA CCATGAACAT
TGTTGCTGGA ACTCTGAGCA TGTCACCTTT GAAGAGAGAG ACAAATGTCC AGAGTGGAAT
AGTTGGTCCC AGCTTATCAT CAGCACAGAT GAGGGAGCCT TTGCCTACAT AGTCAATTAT
TTCATGTACG TCCTCTGGGC TCTCCTATTT GCCTTCCTTG CCGTATCTCT TGTCAAGGTG
TTTGCGCCTT ATGCCTGTGG CTCTGGAATC CCTGAGATAA AAACTATCTT GAGTGGTTTC
ATTATTAGGG GCTATTTGGG TAAGTGGACT CTGGTTATCA AAACCATCAC CTTGGTGCTG
GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA GAGGGCCCTC TAGTGCACGT GGCTTGCTGC
TGTGGGAACA TCCTGTGCCA CTGCTTCAAC AAATACAGGA AGAATGAAGC CAAGCGCAGA
GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT GTATCTGTAG CCTTTGGAGC ACCTATAGGT
GGAGTATTAT TCAGCCTTGA AGAGGTCAGC TACTATTTTC CCCTCAAAAC ATTGTGGCGT
TCATTCTTTG CTGCCTTGGT GGCAGCATTC ACTCTACGCT CCATCAATCC ATTTGGGAAC
AGCCGCCTGG TACTATTTTA TGTGGAGTTT CACACCCCAT GGCATCTCTT TGAGCTCGTG
CCATTCATTC TGCTGGGCAT ATTTGGTGGT CTGTGGGGAG CACTGTTTAT CCGCACAAAC
ATTGCCTGGT GTCGGAAGCG AAAGACCACC CAGTTGGGCA AGTATCCTGT TATAGAGGTA
CTCGTCGTGA CAGCCATCAC TGCCATCCTG GCTTTCCCCA ATGAATACAC TCGGATGAGC
ACAAGTGAGC TCATTTCTGA GCTGTTTAAT GACTGTGGCC TTCTGGACTC CTCCAAGCTC
TGTGATTATG AGAACCGTTT CAACACAAGC AAAGGGGGTG AACTGCCTGA CAGACCGGCT
GGCGTGGGAG TCTACAGTGC AATGTGGCAG CTGGCTTTAA CACTCATACT GAAAATTGTC
ATTACTATAT TCACCTTTGG CATGAAGATC CCTTCTGGCC TCTTTATCCC TAGCATGGCT
GTTGGTGCTA TAGCAGGTCG ACTTCTAGGA GTAGGAATGG AACAGCTGGC TTATTACCAC
CAGGAATGGA CCGTCTTCAA TAGCTGGTGT AGTCAGGGAG CTGATTGCAT CACCCCCGGC
CTTTATGCAA TGGTTGGGGC TGCAGCCTGC TTAGGTGGGG TGACTCGGAT GACTGTTTCT
CTTGTTGTCA TAATGTTTGA ACTGACTGGT GGCTTAGAAT ACATCGTGCC TCTGATGGCT
GCAGCCATGA CAAGCAAGTG GGTGGCAGAT GCTCTTGGGC GGGAGGGCAT CTATGATGCC
CACATCCGTC TCAATGGATA CCCCTTTCTT GAAGCCAAAG AAGAGTTTGC TCATAAGACC
CTGGCAATGG ATGTGATGAA ACCCCGGAGA AATGATCCTT TGTTGACTGT CCTTACTCAG
GACAGTATGA CTGTGGAAGA TGTAGAGACC ATAATCAGTG AAACCACTTA CAGTGGCTTC
CCAGTGGTGG TATCCCGGGA GTCCCAAAGA CTTGTGGGCT TTGTCCTCCG AAGAGATCTC
ATTATTTCAA TTGAAAATGC TCGAAAGAAA CAGGATGGGG TTGTTAGCAC TTCCATCATT
TATTTCACGG AGCATTCTCC TCCATTGCCA CCATACACTC CACCCACTCT AAAGCTTCGG
AACATCCTCG ATCTCAGCCC CTTCACTGTG ACTGACCTTA CACCCATGGA GATCGTAGTG
GATATTTTCC GAAAGCTGGG ACTGCGGCAG TGCCTGGTTA CACACAACGG GCGATTGCTT
GGAATCATTA CCAAAAAGGA TGTGTTAAAG CATATAGCAC AGATGGCGAA CCAAGATCCT
GATTCCATTC TCTTCAACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56926
Accession Version XM_011543888.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product H(+)/Cl(-) exchange transporter 5 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGCCATGT GGCAGGGTGC CATGGATAAC AGAGGCTTTC AGCAGGGGAG TTTTAGTAGC 
TTCCAGAACA GCTCCAGTGA TGAAGACCTG ATGGACATTC CAGCAACCGC TATGGATTTC
TCCATGAGAG ATGATGTTCC TCCCTTAGAC CGAGAAGTAG GAGAGGACAA GTCGTACAAT
GGTGGAGGAA TAGGTTCTTC AAATAGGATC ATGGACTTCT TGGAGGAGCC AATCCCTGGT
GTAGGGACCT ATGATGATTT CAATACAATT GATTGGGTGA GAGAGAAGTC TCGAGACCGG
GATAGGCACC GAGAGATTAC CAATAAAAGC AAAGAGTCAA CATGGGCCTT AATTCACAGT
GTGAGTGATG CTTTTTCCGG CTGGTTGTTG ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG
TTAGCTGGTT TGATAGACAT CTCTGCTCAT TGGATGACAG ACTTAAAAGA AGGTATATGC
ACAGGGGGAT TCTGGTTTAA CCATGAACAT TGTTGCTGGA ACTCTGAGCA TGTCACCTTT
GAAGAGAGAG ACAAATGTCC AGAGTGGAAT AGTTGGTCCC AGCTTATCAT CAGCACAGAT
GAGGGAGCCT TTGCCTACAT AGTCAATTAT TTCATGTACG TCCTCTGGGC TCTCCTATTT
GCCTTCCTTG CCGTATCTCT TGTCAAGGTG TTTGCGCCTT ATGCCTGTGG CTCTGGAATC
CCTGAGATAA AAACTATCTT GAGTGGTTTC ATTATTAGGG GCTATTTGGG TAAGTGGACT
CTGGTTATCA AAACCATCAC CTTGGTGCTG GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA
GAGGGCCCTC TAGTGCACGT GGCTTGCTGC TGTGGGAACA TCCTGTGCCA CTGCTTCAAC
AAATACAGGA AGAATGAAGC CAAGCGCAGA GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT
GTATCTGTAG CCTTTGGAGC ACCTATAGGT GGAGTATTAT TCAGCCTTGA AGAGGTCAGC
TACTATTTTC CCCTCAAAAC ATTGTGGCGT TCATTCTTTG CTGCCTTGGT GGCAGCATTC
ACTCTACGCT CCATCAATCC ATTTGGGAAC AGCCGCCTGG TACTATTTTA TGTGGAGTTT
CACACCCCAT GGCATCTCTT TGAGCTCGTG CCATTCATTC TGCTGGGCAT ATTTGGTGGT
CTGTGGGGAG CACTGTTTAT CCGCACAAAC ATTGCCTGGT GTCGGAAGCG AAAGACCACC
CAGTTGGGCA AGTATCCTGT TATAGAGGTA CTCGTCGTGA CAGCCATCAC TGCCATCCTG
GCTTTCCCCA ATGAATACAC TCGGATGAGC ACAAGTGAGC TCATTTCTGA GCTGTTTAAT
GACTGTGGCC TTCTGGACTC CTCCAAGCTC TGTGATTATG AGAACCGTTT CAACACAAGC
AAAGGGGGTG AACTGCCTGA CAGACCGGCT GGCGTGGGAG TCTACAGTGC AATGTGGCAG
CTGGCTTTAA CACTCATACT GAAAATTGTC ATTACTATAT TCACCTTTGG CATGAAGGCC
CAATTTCTGC CTCCAGTTCT GTGTTGGTCT GGAGAGTTCA GGCTCTTGCC TGATGGACCC
CGAATCAGGA TCCCTTCTGG CCTCTTTATC CCTAGCATGG CTGTTGGTGC TATAGCAGGT
CGACTTCTAG GAGTAGGAAT GGAACAGCTG GCTTATTACC ACCAGGAATG GACCGTCTTC
AATAGCTGGT GTAGTCAGGG AGCTGATTGC ATCACCCCCG GCCTTTATGC AATGGTTGGG
GCTGCAGCCT GCTTAGGTGG GGTGACTCGG ATGACTGTTT CTCTTGTTGT CATAATGTTT
GAACTGACTG GTGGCTTAGA ATACATCGTG CCTCTGATGG CTGCAGCCAT GACAAGCAAG
TGGGTGGCAG ATGCTCTTGG GCGGGAGGGC ATCTATGATG CCCACATCCG TCTCAATGGA
TACCCCTTTC TTGAAGCCAA AGAAGAGTTT GCTCATAAGA CCCTGGCAAT GGATGTGATG
AAACCCCGGA GAAATGATCC TTTGTTGACT GTCCTTACTC AGGACAGTAT GACTGTGGAA
GATGTAGAGA CCATAATCAG TGAAACCACT TACAGTGGCT TCCCAGTGGT GGTATCCCGG
GAGTCCCAAA GACTTGTGGG CTTTGTCCTC CGAAGAGATC TCATTATTTC AATTGAAAAT
GCTCGAAAGA AACAGGATGG GGTTGTTAGC ACTTCCATCA TTTATTTCAC GGAGCATTCT
CCTCCATTGC CACCATACAC TCCACCCACT CTAAAGCTTC GGAACATCCT CGATCTCAGC
CCCTTCACTG TGACTGACCT TACACCCATG GAGATCGTAG TGGATATTTT CCGAAAGCTG
GGACTGCGGC AGTGCCTGGT TACACACAAC GGGCGATTGC TTGGAATCAT TACCAAAAAG
GATGTGTTAA AGCATATAGC ACAGATGGCG AACCAAGATC CTGATTCCAT TCTCTTCAAC
TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011542190.1
CDS333..2855
Misc Feature(1)756..2324(+)
Misc Feature(2)1041..2288(+)
Misc Feature(3)1173..1175(+)
Misc Feature(4)1344..1346(+)
Misc Feature(5)2361..2801(+)
Misc Feature(6)2397..2807(+)
Translation
Position Chain Variation Link
440 440 c, t dbSNP:111464924
712 712 g, t dbSNP:151340629
749 749 c, t dbSNP:113780806
886 886 a, g dbSNP:55676763
1141 1141 g, t dbSNP:151340622
1199 1199 c, t dbSNP:387907402
1216 1216 c, t dbSNP:273585645
1273 1273 c, t dbSNP:151340626
1321 1321 g, t dbSNP:151340630
1357 1357 a, g dbSNP:273585644
1376 1376 c, g dbSNP:273585648
1379 1379 a, g dbSNP:151340620
1381 1381 a, c, g dbSNP:151340628
1562 1562 a, c dbSNP:273585646
1570 1570 a, g dbSNP:151340627
2131 2131 a, g dbSNP:151340625
2151 2151 a, g dbSNP:273585647
2172 2172 c, t dbSNP:151340623
2232 2232 a, g dbSNP:387907403
2251 2251 a, g dbSNP:273585649
2253 2253 g, t dbSNP:273585650
2388 2388 a, g dbSNP:387907404
2556 2556 a, c, t dbSNP:151340621
2724 2724 c, t dbSNP:151340624
3204 3204 a, g dbSNP:111438914
4305 4305 c, t dbSNP:12835125
4455 4455 a, g dbSNP:12859644
5306 5306 a, c dbSNP:12687955
5315 5315 a, g dbSNP:111452206
5404 5404 c, t dbSNP:111369735
5835 5835 a, g dbSNP:56147923
6110 6110 c, t dbSNP:112868450
6264 6264 a, g dbSNP:59126550
6507 6507 c, t dbSNP:56657044
7686 7686 g, t dbSNP:12845771
7688 7688 a, g dbSNP:12837391
7999 7999 c, t dbSNP:111560086
9018 9018 c, t dbSNP:41312128

Target ORF information:

RefSeq Version XM_011543888
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543888

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGCCATGT GGCAGGGTGC CATGGATAAC AGAGGCTTTC AGCAGGGGAG TTTTAGTAGC 
TTCCAGAACA GCTCCAGTGA TGAAGACCTG ATGGACATTC CAGCAACCGC TATGGATTTC
TCCATGAGAG ATGATGTTCC TCCCTTAGAC CGAGAAGTAG GAGAGGACAA GTCGTACAAT
GGTGGAGGAA TAGGTTCTTC AAATAGGATC ATGGACTTCT TGGAGGAGCC AATCCCTGGT
GTAGGGACCT ATGATGATTT CAATACAATT GATTGGGTGA GAGAGAAGTC TCGAGACCGG
GATAGGCACC GAGAGATTAC CAATAAAAGC AAAGAGTCAA CATGGGCCTT AATTCACAGT
GTGAGTGATG CTTTTTCCGG CTGGTTGTTG ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG
TTAGCTGGTT TGATAGACAT CTCTGCTCAT TGGATGACAG ACTTAAAAGA AGGTATATGC
ACAGGGGGAT TCTGGTTTAA CCATGAACAT TGTTGCTGGA ACTCTGAGCA TGTCACCTTT
GAAGAGAGAG ACAAATGTCC AGAGTGGAAT AGTTGGTCCC AGCTTATCAT CAGCACAGAT
GAGGGAGCCT TTGCCTACAT AGTCAATTAT TTCATGTACG TCCTCTGGGC TCTCCTATTT
GCCTTCCTTG CCGTATCTCT TGTCAAGGTG TTTGCGCCTT ATGCCTGTGG CTCTGGAATC
CCTGAGATAA AAACTATCTT GAGTGGTTTC ATTATTAGGG GCTATTTGGG TAAGTGGACT
CTGGTTATCA AAACCATCAC CTTGGTGCTG GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA
GAGGGCCCTC TAGTGCACGT GGCTTGCTGC TGTGGGAACA TCCTGTGCCA CTGCTTCAAC
AAATACAGGA AGAATGAAGC CAAGCGCAGA GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT
GTATCTGTAG CCTTTGGAGC ACCTATAGGT GGAGTATTAT TCAGCCTTGA AGAGGTCAGC
TACTATTTTC CCCTCAAAAC ATTGTGGCGT TCATTCTTTG CTGCCTTGGT GGCAGCATTC
ACTCTACGCT CCATCAATCC ATTTGGGAAC AGCCGCCTGG TACTATTTTA TGTGGAGTTT
CACACCCCAT GGCATCTCTT TGAGCTCGTG CCATTCATTC TGCTGGGCAT ATTTGGTGGT
CTGTGGGGAG CACTGTTTAT CCGCACAAAC ATTGCCTGGT GTCGGAAGCG AAAGACCACC
CAGTTGGGCA AGTATCCTGT TATAGAGGTA CTCGTCGTGA CAGCCATCAC TGCCATCCTG
GCTTTCCCCA ATGAATACAC TCGGATGAGC ACAAGTGAGC TCATTTCTGA GCTGTTTAAT
GACTGTGGCC TTCTGGACTC CTCCAAGCTC TGTGATTATG AGAACCGTTT CAACACAAGC
AAAGGGGGTG AACTGCCTGA CAGACCGGCT GGCGTGGGAG TCTACAGTGC AATGTGGCAG
CTGGCTTTAA CACTCATACT GAAAATTGTC ATTACTATAT TCACCTTTGG CATGAAGGCC
CAATTTCTGC CTCCAGTTCT GTGTTGGTCT GGAGAGTTCA GGCTCTTGCC TGATGGACCC
CGAATCAGGA TCCCTTCTGG CCTCTTTATC CCTAGCATGG CTGTTGGTGC TATAGCAGGT
CGACTTCTAG GAGTAGGAAT GGAACAGCTG GCTTATTACC ACCAGGAATG GACCGTCTTC
AATAGCTGGT GTAGTCAGGG AGCTGATTGC ATCACCCCCG GCCTTTATGC AATGGTTGGG
GCTGCAGCCT GCTTAGGTGG GGTGACTCGG ATGACTGTTT CTCTTGTTGT CATAATGTTT
GAACTGACTG GTGGCTTAGA ATACATCGTG CCTCTGATGG CTGCAGCCAT GACAAGCAAG
TGGGTGGCAG ATGCTCTTGG GCGGGAGGGC ATCTATGATG CCCACATCCG TCTCAATGGA
TACCCCTTTC TTGAAGCCAA AGAAGAGTTT GCTCATAAGA CCCTGGCAAT GGATGTGATG
AAACCCCGGA GAAATGATCC TTTGTTGACT GTCCTTACTC AGGACAGTAT GACTGTGGAA
GATGTAGAGA CCATAATCAG TGAAACCACT TACAGTGGCT TCCCAGTGGT GGTATCCCGG
GAGTCCCAAA GACTTGTGGG CTTTGTCCTC CGAAGAGATC TCATTATTTC AATTGAAAAT
GCTCGAAAGA AACAGGATGG GGTTGTTAGC ACTTCCATCA TTTATTTCAC GGAGCATTCT
CCTCCATTGC CACCATACAC TCCACCCACT CTAAAGCTTC GGAACATCCT CGATCTCAGC
CCCTTCACTG TGACTGACCT TACACCCATG GAGATCGTAG TGGATATTTT CCGAAAGCTG
GGACTGCGGC AGTGCCTGGT TACACACAAC GGGCGATTGC TTGGAATCAT TACCAAAAAG
GATGTGTTAA AGCATATAGC ACAGATGGCG AACCAAGATC CTGATTCCAT TCTCTTCAAC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56927
Accession Version XM_011543889.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product H(+)/Cl(-) exchange transporter 5 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGACTTCT TGGAGGAGCC AATCCCTGGT GTAGGGACCT ATGATGATTT CAATACAATT 
GATTGGGTGA GAGAGAAGTC TCGAGACCGG GATAGGCACC GAGAGATTAC CAATAAAAGC
AAAGAGTCAA CATGGGCCTT AATTCACAGT GTGAGTGATG CTTTTTCCGG CTGGTTGTTG
ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG TTAGCTGGTT TGATAGACAT CTCTGCTCAT
TGGATGACAG ACTTAAAAGA AGGTATATGC ACAGGGGGAT TCTGGTTTAA CCATGAACAT
TGTTGCTGGA ACTCTGAGCA TGTCACCTTT GAAGAGAGAG ACAAATGTCC AGAGTGGAAT
AGTTGGTCCC AGCTTATCAT CAGCACAGAT GAGGGAGCCT TTGCCTACAT AGTCAATTAT
TTCATGTACG TCCTCTGGGC TCTCCTATTT GCCTTCCTTG CCGTATCTCT TGTCAAGGTG
TTTGCGCCTT ATGCCTGTGG CTCTGGAATC CCTGAGATAA AAACTATCTT GAGTGGTTTC
ATTATTAGGG GCTATTTGGG TAAGTGGACT CTGGTTATCA AAACCATCAC CTTGGTGCTG
GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA GAGGGCCCTC TAGTGCACGT GGCTTGCTGC
TGTGGGAACA TCCTGTGCCA CTGCTTCAAC AAATACAGGA AGAATGAAGC CAAGCGCAGA
GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT GTATCTGTAG CCTTTGGAGC ACCTATAGGT
GGAGTATTAT TCAGCCTTGA AGAGGTCAGC TACTATTTTC CCCTCAAAAC ATTGTGGCGT
TCATTCTTTG CTGCCTTGGT GGCAGCATTC ACTCTACGCT CCATCAATCC ATTTGGGAAC
AGCCGCCTGG TACTATTTTA TGTGGAGTTT CACACCCCAT GGCATCTCTT TGAGCTCGTG
CCATTCATTC TGCTGGGCAT ATTTGGTGGT CTGTGGGGAG CACTGTTTAT CCGCACAAAC
ATTGCCTGGT GTCGGAAGCG AAAGACCACC CAGTTGGGCA AGTATCCTGT TATAGAGGTA
CTCGTCGTGA CAGCCATCAC TGCCATCCTG GCTTTCCCCA ATGAATACAC TCGGATGAGC
ACAAGTGAGC TCATTTCTGA GCTGTTTAAT GACTGTGGCC TTCTGGACTC CTCCAAGCTC
TGTGATTATG AGAACCGTTT CAACACAAGC AAAGGGGGTG AACTGCCTGA CAGACCGGCT
GGCGTGGGAG TCTACAGTGC AATGTGGCAG CTGGCTTTAA CACTCATACT GAAAATTGTC
ATTACTATAT TCACCTTTGG CATGAAGGCC CAATTTCTGC CTCCAGTTCT GTGTTGGTCT
GGAGAGTTCA GGCTCTTGCC TGATGGACCC CGAATCAGGA TCCCTTCTGG CCTCTTTATC
CCTAGCATGG CTGTTGGTGC TATAGCAGGT CGACTTCTAG GAGTAGGAAT GGAACAGCTG
GCTTATTACC ACCAGGAATG GACCGTCTTC AATAGCTGGT GTAGTCAGGG AGCTGATTGC
ATCACCCCCG GCCTTTATGC AATGGTTGGG GCTGCAGCCT GCTTAGGTGG GGTGACTCGG
ATGACTGTTT CTCTTGTTGT CATAATGTTT GAACTGACTG GTGGCTTAGA ATACATCGTG
CCTCTGATGG CTGCAGCCAT GACAAGCAAG TGGGTGGCAG ATGCTCTTGG GCGGGAGGGC
ATCTATGATG CCCACATCCG TCTCAATGGA TACCCCTTTC TTGAAGCCAA AGAAGAGTTT
GCTCATAAGA CCCTGGCAAT GGATGTGATG AAACCCCGGA GAAATGATCC TTTGTTGACT
GTCCTTACTC AGGACAGTAT GACTGTGGAA GATGTAGAGA CCATAATCAG TGAAACCACT
TACAGTGGCT TCCCAGTGGT GGTATCCCGG GAGTCCCAAA GACTTGTGGG CTTTGTCCTC
CGAAGAGATC TCATTATTTC AATTGAAAAT GCTCGAAAGA AACAGGATGG GGTTGTTAGC
ACTTCCATCA TTTATTTCAC GGAGCATTCT CCTCCATTGC CACCATACAC TCCACCCACT
CTAAAGCTTC GGAACATCCT CGATCTCAGC CCCTTCACTG TGACTGACCT TACACCCATG
GAGATCGTAG TGGATATTTT CCGAAAGCTG GGACTGCGGC AGTGCCTGGT TACACACAAC
GGGCGATTGC TTGGAATCAT TACCAAAAAG GATGTGTTAA AGCATATAGC ACAGATGGCG
AACCAAGATC CTGATTCCAT TCTCTTCAAC TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011542191.1
CDS248..2560
Misc Feature(1)461..2029(+)
Misc Feature(2)746..1993(+)
Misc Feature(3)878..880(+)
Misc Feature(4)1049..1051(+)
Misc Feature(5)2066..2506(+)
Misc Feature(6)2102..2512(+)
Translation
Position Chain Variation Link
417 417 g, t dbSNP:151340629
454 454 c, t dbSNP:113780806
591 591 a, g dbSNP:55676763
846 846 g, t dbSNP:151340622
904 904 c, t dbSNP:387907402
921 921 c, t dbSNP:273585645
978 978 c, t dbSNP:151340626
1026 1026 g, t dbSNP:151340630
1062 1062 a, g dbSNP:273585644
1081 1081 c, g dbSNP:273585648
1084 1084 a, g dbSNP:151340620
1086 1086 a, c, g dbSNP:151340628
1267 1267 a, c dbSNP:273585646
1275 1275 a, g dbSNP:151340627
1836 1836 a, g dbSNP:151340625
1856 1856 a, g dbSNP:273585647
1877 1877 c, t dbSNP:151340623
1937 1937 a, g dbSNP:387907403
1956 1956 a, g dbSNP:273585649
1958 1958 g, t dbSNP:273585650
2093 2093 a, g dbSNP:387907404
2261 2261 a, c, t dbSNP:151340621
2429 2429 c, t dbSNP:151340624
2909 2909 a, g dbSNP:111438914
4010 4010 c, t dbSNP:12835125
4160 4160 a, g dbSNP:12859644
5011 5011 a, c dbSNP:12687955
5020 5020 a, g dbSNP:111452206
5109 5109 c, t dbSNP:111369735
5540 5540 a, g dbSNP:56147923
5815 5815 c, t dbSNP:112868450
5969 5969 a, g dbSNP:59126550
6212 6212 c, t dbSNP:56657044
7391 7391 g, t dbSNP:12845771
7393 7393 a, g dbSNP:12837391
7704 7704 c, t dbSNP:111560086
8723 8723 c, t dbSNP:41312128

Target ORF information:

RefSeq Version XM_011543889
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543889

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGACTTCT TGGAGGAGCC AATCCCTGGT GTAGGGACCT ATGATGATTT CAATACAATT 
GATTGGGTGA GAGAGAAGTC TCGAGACCGG GATAGGCACC GAGAGATTAC CAATAAAAGC
AAAGAGTCAA CATGGGCCTT AATTCACAGT GTGAGTGATG CTTTTTCCGG CTGGTTGTTG
ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG TTAGCTGGTT TGATAGACAT CTCTGCTCAT
TGGATGACAG ACTTAAAAGA AGGTATATGC ACAGGGGGAT TCTGGTTTAA CCATGAACAT
TGTTGCTGGA ACTCTGAGCA TGTCACCTTT GAAGAGAGAG ACAAATGTCC AGAGTGGAAT
AGTTGGTCCC AGCTTATCAT CAGCACAGAT GAGGGAGCCT TTGCCTACAT AGTCAATTAT
TTCATGTACG TCCTCTGGGC TCTCCTATTT GCCTTCCTTG CCGTATCTCT TGTCAAGGTG
TTTGCGCCTT ATGCCTGTGG CTCTGGAATC CCTGAGATAA AAACTATCTT GAGTGGTTTC
ATTATTAGGG GCTATTTGGG TAAGTGGACT CTGGTTATCA AAACCATCAC CTTGGTGCTG
GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA GAGGGCCCTC TAGTGCACGT GGCTTGCTGC
TGTGGGAACA TCCTGTGCCA CTGCTTCAAC AAATACAGGA AGAATGAAGC CAAGCGCAGA
GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT GTATCTGTAG CCTTTGGAGC ACCTATAGGT
GGAGTATTAT TCAGCCTTGA AGAGGTCAGC TACTATTTTC CCCTCAAAAC ATTGTGGCGT
TCATTCTTTG CTGCCTTGGT GGCAGCATTC ACTCTACGCT CCATCAATCC ATTTGGGAAC
AGCCGCCTGG TACTATTTTA TGTGGAGTTT CACACCCCAT GGCATCTCTT TGAGCTCGTG
CCATTCATTC TGCTGGGCAT ATTTGGTGGT CTGTGGGGAG CACTGTTTAT CCGCACAAAC
ATTGCCTGGT GTCGGAAGCG AAAGACCACC CAGTTGGGCA AGTATCCTGT TATAGAGGTA
CTCGTCGTGA CAGCCATCAC TGCCATCCTG GCTTTCCCCA ATGAATACAC TCGGATGAGC
ACAAGTGAGC TCATTTCTGA GCTGTTTAAT GACTGTGGCC TTCTGGACTC CTCCAAGCTC
TGTGATTATG AGAACCGTTT CAACACAAGC AAAGGGGGTG AACTGCCTGA CAGACCGGCT
GGCGTGGGAG TCTACAGTGC AATGTGGCAG CTGGCTTTAA CACTCATACT GAAAATTGTC
ATTACTATAT TCACCTTTGG CATGAAGGCC CAATTTCTGC CTCCAGTTCT GTGTTGGTCT
GGAGAGTTCA GGCTCTTGCC TGATGGACCC CGAATCAGGA TCCCTTCTGG CCTCTTTATC
CCTAGCATGG CTGTTGGTGC TATAGCAGGT CGACTTCTAG GAGTAGGAAT GGAACAGCTG
GCTTATTACC ACCAGGAATG GACCGTCTTC AATAGCTGGT GTAGTCAGGG AGCTGATTGC
ATCACCCCCG GCCTTTATGC AATGGTTGGG GCTGCAGCCT GCTTAGGTGG GGTGACTCGG
ATGACTGTTT CTCTTGTTGT CATAATGTTT GAACTGACTG GTGGCTTAGA ATACATCGTG
CCTCTGATGG CTGCAGCCAT GACAAGCAAG TGGGTGGCAG ATGCTCTTGG GCGGGAGGGC
ATCTATGATG CCCACATCCG TCTCAATGGA TACCCCTTTC TTGAAGCCAA AGAAGAGTTT
GCTCATAAGA CCCTGGCAAT GGATGTGATG AAACCCCGGA GAAATGATCC TTTGTTGACT
GTCCTTACTC AGGACAGTAT GACTGTGGAA GATGTAGAGA CCATAATCAG TGAAACCACT
TACAGTGGCT TCCCAGTGGT GGTATCCCGG GAGTCCCAAA GACTTGTGGG CTTTGTCCTC
CGAAGAGATC TCATTATTTC AATTGAAAAT GCTCGAAAGA AACAGGATGG GGTTGTTAGC
ACTTCCATCA TTTATTTCAC GGAGCATTCT CCTCCATTGC CACCATACAC TCCACCCACT
CTAAAGCTTC GGAACATCCT CGATCTCAGC CCCTTCACTG TGACTGACCT TACACCCATG
GAGATCGTAG TGGATATTTT CCGAAAGCTG GGACTGCGGC AGTGCCTGGT TACACACAAC
GGGCGATTGC TTGGAATCAT TACCAAAAAG GATGTGTTAA AGCATATAGC ACAGATGGCG
AACCAAGATC CTGATTCCAT TCTCTTCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21666
Accession Version NM_001127899.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product H(+)/Cl(-) exchange transporter 5 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BK000969.1, DA737534.1, AK092049.1, AC231645.2, BC051691.1, AK025562.1 and AA743724.1. This sequence is a reference standard in the RefSeqGene project. On Aug 21, 2013 this sequence version replaced gi:442535511. Summary: This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]. Transcript Variant: This variant (1, also known as type 2) represents the longest transcript and encodes the longest isoform (a). Both variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCATGT GGCAGGGTGC CATGGATAAC AGAGGCTTTC AGCAGGGGAG TTTTAGTAGC 
TTCCAGAACA GCTCCAGTGA TGAAGACCTG ATGGACATTC CAGCAACCGC TATGGATTTC
TCCATGAGAG ATGATGTTCC TCCCTTAGAC CGAGAAGTAG GAGAGGACAA GTCGTACAAT
GGTGGAGGAA TAGGTTCTTC AAATAGGATC ATGGACTTCT TGGAGGAGCC AATCCCTGGT
GTAGGGACCT ATGATGATTT CAATACAATT GATTGGGTGA GAGAGAAGTC TCGAGACCGG
GATAGGCACC GAGAGATTAC CAATAAAAGC AAAGAGTCAA CATGGGCCTT AATTCACAGT
GTGAGTGATG CTTTTTCCGG CTGGTTGTTG ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG
TTAGCTGGTT TGATAGACAT CTCTGCTCAT TGGATGACAG ACTTAAAAGA AGGTATATGC
ACAGGGGGAT TCTGGTTTAA CCATGAACAT TGTTGCTGGA ACTCTGAGCA TGTCACCTTT
GAAGAGAGAG ACAAATGTCC AGAGTGGAAT AGTTGGTCCC AGCTTATCAT CAGCACAGAT
GAGGGAGCCT TTGCCTACAT AGTCAATTAT TTCATGTACG TCCTCTGGGC TCTCCTATTT
GCCTTCCTTG CCGTATCTCT TGTCAAGGTG TTTGCGCCTT ATGCCTGTGG CTCTGGAATC
CCTGAGATAA AAACTATCTT GAGTGGTTTC ATTATTAGGG GCTATTTGGG TAAGTGGACT
CTGGTTATCA AAACCATCAC CTTGGTGCTG GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA
GAGGGCCCTC TAGTGCACGT GGCTTGCTGC TGTGGGAACA TCCTGTGCCA CTGCTTCAAC
AAATACAGGA AGAATGAAGC CAAGCGCAGA GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT
GTATCTGTAG CCTTTGGAGC ACCTATAGGT GGAGTATTAT TCAGCCTTGA AGAGGTCAGC
TACTATTTTC CCCTCAAAAC ATTGTGGCGT TCATTCTTTG CTGCCTTGGT GGCAGCATTC
ACTCTACGCT CCATCAATCC ATTTGGGAAC AGCCGCCTGG TACTATTTTA TGTGGAGTTT
CACACCCCAT GGCATCTCTT TGAGCTCGTG CCATTCATTC TGCTGGGCAT ATTTGGTGGT
CTGTGGGGAG CACTGTTTAT CCGCACAAAC ATTGCCTGGT GTCGGAAGCG AAAGACCACC
CAGTTGGGCA AGTATCCTGT TATAGAGGTA CTCGTCGTGA CAGCCATCAC TGCCATCCTG
GCTTTCCCCA ATGAATACAC TCGGATGAGC ACAAGTGAGC TCATTTCTGA GCTGTTTAAT
GACTGTGGCC TTCTGGACTC CTCCAAGCTC TGTGATTATG AGAACCGTTT CAACACAAGC
AAAGGGGGTG AACTGCCTGA CAGACCGGCT GGCGTGGGAG TCTACAGTGC AATGTGGCAG
CTGGCTTTAA CACTCATACT GAAAATTGTC ATTACTATAT TCACCTTTGG CATGAAGATC
CCTTCTGGCC TCTTTATCCC TAGCATGGCT GTTGGTGCTA TAGCAGGTCG ACTTCTAGGA
GTAGGAATGG AACAGCTGGC TTATTACCAC CAGGAATGGA CCGTCTTCAA TAGCTGGTGT
AGTCAGGGAG CTGATTGCAT CACCCCCGGC CTTTATGCAA TGGTTGGGGC TGCAGCCTGC
TTAGGTGGGG TGACTCGGAT GACTGTTTCT CTTGTTGTCA TAATGTTTGA ACTGACTGGT
GGCTTAGAAT ACATCGTGCC TCTGATGGCT GCAGCCATGA CAAGCAAGTG GGTGGCAGAT
GCTCTTGGGC GGGAGGGCAT CTATGATGCC CACATCCGTC TCAATGGATA CCCCTTTCTT
GAAGCCAAAG AAGAGTTTGC TCATAAGACC CTGGCAATGG ATGTGATGAA ACCCCGGAGA
AATGATCCTT TGTTGACTGT CCTTACTCAG GACAGTATGA CTGTGGAAGA TGTAGAGACC
ATAATCAGTG AAACCACTTA CAGTGGCTTC CCAGTGGTGG TATCCCGGGA GTCCCAAAGA
CTTGTGGGCT TTGTCCTCCG AAGAGATCTC ATTATTTCAA TTGAAAATGC TCGAAAGAAA
CAGGATGGGG TTGTTAGCAC TTCCATCATT TATTTCACGG AGCATTCTCC TCCATTGCCA
CCATACACTC CACCCACTCT AAAGCTTCGG AACATCCTCG ATCTCAGCCC CTTCACTGTG
ACTGACCTTA CACCCATGGA GATCGTAGTG GATATTTTCC GAAAGCTGGG ACTGCGGCAG
TGCCTGGTTA CACACAACGG GCGATTGCTT GGAATCATTA CCAAAAAGGA TGTGTTAAAG
CATATAGCAC AGATGGCGAA CCAAGATCCT GATTCCATTC TCTTCAACTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001121371.1
CDS642..3092
Misc Feature(1)585..587(+)
Misc Feature(2)1065..2561(+)
Misc Feature(3)1350..2525(+)
Misc Feature(4)1482..1484(+)
Misc Feature(5)1653..1655(+)
Misc Feature(6)2598..3038(+)
Misc Feature(7)2634..3044(+)
Exon (1)1..178
Gene:CLCN5
Gene Synonym:
Exon (2)179..513
Gene:CLCN5
Gene Synonym:
Exon (3)514..657
Gene:CLCN5
Gene Synonym:
Exon (4)658..804
Gene:CLCN5
Gene Synonym:
Exon (5)805..956
Gene:CLCN5
Gene Synonym:
Exon (6)957..1056
Gene:CLCN5
Gene Synonym:
Exon (7)1057..1244
Gene:CLCN5
Gene Synonym:
Exon (8)1245..1367
Gene:CLCN5
Gene Synonym:
Exon (9)1368..1574
Gene:CLCN5
Gene Synonym:
Exon (10)1575..1655
Gene:CLCN5
Gene Synonym:
Exon (11)1656..2198
Gene:CLCN5
Gene Synonym:
Exon (12)2199..2385
Gene:CLCN5
Gene Synonym:
Exon (13)2386..2784
Gene:CLCN5
Gene Synonym:
Exon (14)2785..3001
Gene:CLCN5
Gene Synonym:
Exon (15)3002..10108
Gene:CLCN5
Gene Synonym:
Translation
Position Chain Variation Link
41 41 -, c dbSNP:142374236
476 476 c, t dbSNP:111747255
749 749 c, t dbSNP:111464924
1021 1021 g, t dbSNP:151340629
1058 1058 c, t dbSNP:113780806
1195 1195 a, g dbSNP:55676763
1450 1450 g, t dbSNP:151340622
1508 1508 c, t dbSNP:387907402
1525 1525 c, t dbSNP:273585645
1582 1582 c, t dbSNP:151340626
1630 1630 g, t dbSNP:151340630
1666 1666 a, g dbSNP:273585644
1685 1685 c, g dbSNP:273585648
1688 1688 a, g dbSNP:151340620
1690 1690 a, c, g dbSNP:151340628
1871 1871 a, c dbSNP:273585646
1879 1879 a, g dbSNP:151340627
2368 2368 a, g dbSNP:151340625
2388 2388 a, g dbSNP:273585647
2409 2409 c, t dbSNP:151340623
2469 2469 a, g dbSNP:387907403
2488 2488 a, g dbSNP:273585649
2490 2490 g, t dbSNP:273585650
2625 2625 a, g dbSNP:387907404
2793 2793 a, c, t dbSNP:151340621
2961 2961 c, t dbSNP:151340624
3441 3441 a, g dbSNP:111438914
4542 4542 c, t dbSNP:12835125
4692 4692 a, g dbSNP:12859644
5543 5543 a, c dbSNP:12687955
5552 5552 a, g dbSNP:111452206
5641 5641 c, t dbSNP:111369735
6072 6072 a, g dbSNP:56147923
6347 6347 c, t dbSNP:112868450
6501 6501 a, g dbSNP:59126550
6744 6744 c, t dbSNP:56657044
7923 7923 g, t dbSNP:12845771
7925 7925 a, g dbSNP:12837391
8236 8236 c, t dbSNP:111560086
9255 9255 c, t dbSNP:41312128

Target ORF information:

RefSeq Version NM_001127899
Organism Homo sapiens (human)
Definition Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127899

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCATGT GGCAGGGTGC CATGGATAAC AGAGGCTTTC AGCAGGGGAG TTTTAGTAGC 
TTCCAGAACA GCTCCAGTGA TGAAGACCTG ATGGACATTC CAGCAACCGC TATGGATTTC
TCCATGAGAG ATGATGTTCC TCCCTTAGAC CGAGAAGTAG GAGAGGACAA GTCGTACAAT
GGTGGAGGAA TAGGTTCTTC AAATAGGATC ATGGACTTCT TGGAGGAGCC AATCCCTGGT
GTAGGGACCT ATGATGATTT CAATACAATT GATTGGGTGA GAGAGAAGTC TCGAGACCGG
GATAGGCACC GAGAGATTAC CAATAAAAGC AAAGAGTCAA CATGGGCCTT AATTCACAGT
GTGAGTGATG CTTTTTCCGG CTGGTTGTTG ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG
TTAGCTGGTT TGATAGACAT CTCTGCTCAT TGGATGACAG ACTTAAAAGA AGGTATATGC
ACAGGGGGAT TCTGGTTTAA CCATGAACAT TGTTGCTGGA ACTCTGAGCA TGTCACCTTT
GAAGAGAGAG ACAAATGTCC AGAGTGGAAT AGTTGGTCCC AGCTTATCAT CAGCACAGAT
GAGGGAGCCT TTGCCTACAT AGTCAATTAT TTCATGTACG TCCTCTGGGC TCTCCTATTT
GCCTTCCTTG CCGTATCTCT TGTCAAGGTG TTTGCGCCTT ATGCCTGTGG CTCTGGAATC
CCTGAGATAA AAACTATCTT GAGTGGTTTC ATTATTAGGG GCTATTTGGG TAAGTGGACT
CTGGTTATCA AAACCATCAC CTTGGTGCTG GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA
GAGGGCCCTC TAGTGCACGT GGCTTGCTGC TGTGGGAACA TCCTGTGCCA CTGCTTCAAC
AAATACAGGA AGAATGAAGC CAAGCGCAGA GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT
GTATCTGTAG CCTTTGGAGC ACCTATAGGT GGAGTATTAT TCAGCCTTGA AGAGGTCAGC
TACTATTTTC CCCTCAAAAC ATTGTGGCGT TCATTCTTTG CTGCCTTGGT GGCAGCATTC
ACTCTACGCT CCATCAATCC ATTTGGGAAC AGCCGCCTGG TACTATTTTA TGTGGAGTTT
CACACCCCAT GGCATCTCTT TGAGCTCGTG CCATTCATTC TGCTGGGCAT ATTTGGTGGT
CTGTGGGGAG CACTGTTTAT CCGCACAAAC ATTGCCTGGT GTCGGAAGCG AAAGACCACC
CAGTTGGGCA AGTATCCTGT TATAGAGGTA CTCGTCGTGA CAGCCATCAC TGCCATCCTG
GCTTTCCCCA ATGAATACAC TCGGATGAGC ACAAGTGAGC TCATTTCTGA GCTGTTTAAT
GACTGTGGCC TTCTGGACTC CTCCAAGCTC TGTGATTATG AGAACCGTTT CAACACAAGC
AAAGGGGGTG AACTGCCTGA CAGACCGGCT GGCGTGGGAG TCTACAGTGC AATGTGGCAG
CTGGCTTTAA CACTCATACT GAAAATTGTC ATTACTATAT TCACCTTTGG CATGAAGATC
CCTTCTGGCC TCTTTATCCC TAGCATGGCT GTTGGTGCTA TAGCAGGTCG ACTTCTAGGA
GTAGGAATGG AACAGCTGGC TTATTACCAC CAGGAATGGA CCGTCTTCAA TAGCTGGTGT
AGTCAGGGAG CTGATTGCAT CACCCCCGGC CTTTATGCAA TGGTTGGGGC TGCAGCCTGC
TTAGGTGGGG TGACTCGGAT GACTGTTTCT CTTGTTGTCA TAATGTTTGA ACTGACTGGT
GGCTTAGAAT ACATCGTGCC TCTGATGGCT GCAGCCATGA CAAGCAAGTG GGTGGCAGAT
GCTCTTGGGC GGGAGGGCAT CTATGATGCC CACATCCGTC TCAATGGATA CCCCTTTCTT
GAAGCCAAAG AAGAGTTTGC TCATAAGACC CTGGCAATGG ATGTGATGAA ACCCCGGAGA
AATGATCCTT TGTTGACTGT CCTTACTCAG GACAGTATGA CTGTGGAAGA TGTAGAGACC
ATAATCAGTG AAACCACTTA CAGTGGCTTC CCAGTGGTGG TATCCCGGGA GTCCCAAAGA
CTTGTGGGCT TTGTCCTCCG AAGAGATCTC ATTATTTCAA TTGAAAATGC TCGAAAGAAA
CAGGATGGGG TTGTTAGCAC TTCCATCATT TATTTCACGG AGCATTCTCC TCCATTGCCA
CCATACACTC CACCCACTCT AAAGCTTCGG AACATCCTCG ATCTCAGCCC CTTCACTGTG
ACTGACCTTA CACCCATGGA GATCGTAGTG GATATTTTCC GAAAGCTGGG ACTGCGGCAG
TGCCTGGTTA CACACAACGG GCGATTGCTT GGAATCATTA CCAAAAAGGA TGTGTTAAAG
CATATAGCAC AGATGGCGAA CCAAGATCCT GATTCCATTC TCTTCAACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21666
Accession Version NM_001127898.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product H(+)/Cl(-) exchange transporter 5 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA652242.1, AK056560.1, AC231645.2, BC051691.1, AK025562.1 and AA743724.1. On Aug 21, 2013 this sequence version replaced gi:442535512. Summary: This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]. Transcript Variant: This variant (2, also known as type 1) uses an alternate splice site in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056560.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCATGT GGCAGGGTGC CATGGATAAC AGAGGCTTTC AGCAGGGGAG TTTTAGTAGC 
TTCCAGAACA GCTCCAGTGA TGAAGACCTG ATGGACATTC CAGCAACCGC TATGGATTTC
TCCATGAGAG ATGATGTTCC TCCCTTAGAC CGAGAAGTAG GAGAGGACAA GTCGTACAAT
GGTGGAGGAA TAGGTTCTTC AAATAGGATC ATGGACTTCT TGGAGGAGCC AATCCCTGGT
GTAGGGACCT ATGATGATTT CAATACAATT GATTGGGTGA GAGAGAAGTC TCGAGACCGG
GATAGGCACC GAGAGATTAC CAATAAAAGC AAAGAGTCAA CATGGGCCTT AATTCACAGT
GTGAGTGATG CTTTTTCCGG CTGGTTGTTG ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG
TTAGCTGGTT TGATAGACAT CTCTGCTCAT TGGATGACAG ACTTAAAAGA AGGTATATGC
ACAGGGGGAT TCTGGTTTAA CCATGAACAT TGTTGCTGGA ACTCTGAGCA TGTCACCTTT
GAAGAGAGAG ACAAATGTCC AGAGTGGAAT AGTTGGTCCC AGCTTATCAT CAGCACAGAT
GAGGGAGCCT TTGCCTACAT AGTCAATTAT TTCATGTACG TCCTCTGGGC TCTCCTATTT
GCCTTCCTTG CCGTATCTCT TGTCAAGGTG TTTGCGCCTT ATGCCTGTGG CTCTGGAATC
CCTGAGATAA AAACTATCTT GAGTGGTTTC ATTATTAGGG GCTATTTGGG TAAGTGGACT
CTGGTTATCA AAACCATCAC CTTGGTGCTG GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA
GAGGGCCCTC TAGTGCACGT GGCTTGCTGC TGTGGGAACA TCCTGTGCCA CTGCTTCAAC
AAATACAGGA AGAATGAAGC CAAGCGCAGA GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT
GTATCTGTAG CCTTTGGAGC ACCTATAGGT GGAGTATTAT TCAGCCTTGA AGAGGTCAGC
TACTATTTTC CCCTCAAAAC ATTGTGGCGT TCATTCTTTG CTGCCTTGGT GGCAGCATTC
ACTCTACGCT CCATCAATCC ATTTGGGAAC AGCCGCCTGG TACTATTTTA TGTGGAGTTT
CACACCCCAT GGCATCTCTT TGAGCTCGTG CCATTCATTC TGCTGGGCAT ATTTGGTGGT
CTGTGGGGAG CACTGTTTAT CCGCACAAAC ATTGCCTGGT GTCGGAAGCG AAAGACCACC
CAGTTGGGCA AGTATCCTGT TATAGAGGTA CTCGTCGTGA CAGCCATCAC TGCCATCCTG
GCTTTCCCCA ATGAATACAC TCGGATGAGC ACAAGTGAGC TCATTTCTGA GCTGTTTAAT
GACTGTGGCC TTCTGGACTC CTCCAAGCTC TGTGATTATG AGAACCGTTT CAACACAAGC
AAAGGGGGTG AACTGCCTGA CAGACCGGCT GGCGTGGGAG TCTACAGTGC AATGTGGCAG
CTGGCTTTAA CACTCATACT GAAAATTGTC ATTACTATAT TCACCTTTGG CATGAAGATC
CCTTCTGGCC TCTTTATCCC TAGCATGGCT GTTGGTGCTA TAGCAGGTCG ACTTCTAGGA
GTAGGAATGG AACAGCTGGC TTATTACCAC CAGGAATGGA CCGTCTTCAA TAGCTGGTGT
AGTCAGGGAG CTGATTGCAT CACCCCCGGC CTTTATGCAA TGGTTGGGGC TGCAGCCTGC
TTAGGTGGGG TGACTCGGAT GACTGTTTCT CTTGTTGTCA TAATGTTTGA ACTGACTGGT
GGCTTAGAAT ACATCGTGCC TCTGATGGCT GCAGCCATGA CAAGCAAGTG GGTGGCAGAT
GCTCTTGGGC GGGAGGGCAT CTATGATGCC CACATCCGTC TCAATGGATA CCCCTTTCTT
GAAGCCAAAG AAGAGTTTGC TCATAAGACC CTGGCAATGG ATGTGATGAA ACCCCGGAGA
AATGATCCTT TGTTGACTGT CCTTACTCAG GACAGTATGA CTGTGGAAGA TGTAGAGACC
ATAATCAGTG AAACCACTTA CAGTGGCTTC CCAGTGGTGG TATCCCGGGA GTCCCAAAGA
CTTGTGGGCT TTGTCCTCCG AAGAGATCTC ATTATTTCAA TTGAAAATGC TCGAAAGAAA
CAGGATGGGG TTGTTAGCAC TTCCATCATT TATTTCACGG AGCATTCTCC TCCATTGCCA
CCATACACTC CACCCACTCT AAAGCTTCGG AACATCCTCG ATCTCAGCCC CTTCACTGTG
ACTGACCTTA CACCCATGGA GATCGTAGTG GATATTTTCC GAAAGCTGGG ACTGCGGCAG
TGCCTGGTTA CACACAACGG GCGATTGCTT GGAATCATTA CCAAAAAGGA TGTGTTAAAG
CATATAGCAC AGATGGCGAA CCAAGATCCT GATTCCATTC TCTTCAACTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001121370.1
CDS383..2833
Misc Feature(1)326..328(+)
Misc Feature(2)806..2302(+)
Misc Feature(3)1091..2266(+)
Misc Feature(4)1223..1225(+)
Misc Feature(5)1394..1396(+)
Misc Feature(6)2339..2779(+)
Misc Feature(7)2375..2785(+)
Exon (1)1..178
Gene:CLCN5
Gene Synonym:
Exon (2)179..254
Gene:CLCN5
Gene Synonym:
Exon (3)255..398
Gene:CLCN5
Gene Synonym:
Exon (4)399..545
Gene:CLCN5
Gene Synonym:
Exon (5)546..697
Gene:CLCN5
Gene Synonym:
Exon (6)698..797
Gene:CLCN5
Gene Synonym:
Exon (7)798..985
Gene:CLCN5
Gene Synonym:
Exon (8)986..1108
Gene:CLCN5
Gene Synonym:
Exon (9)1109..1315
Gene:CLCN5
Gene Synonym:
Exon (10)1316..1396
Gene:CLCN5
Gene Synonym:
Exon (11)1397..1939
Gene:CLCN5
Gene Synonym:
Exon (12)1940..2126
Gene:CLCN5
Gene Synonym:
Exon (13)2127..2525
Gene:CLCN5
Gene Synonym:
Exon (14)2526..2742
Gene:CLCN5
Gene Synonym:
Exon (15)2743..9849
Gene:CLCN5
Gene Synonym:
Translation
Position Chain Variation Link
41 41 -, c dbSNP:142374236
490 490 c, t dbSNP:111464924
762 762 g, t dbSNP:151340629
799 799 c, t dbSNP:113780806
936 936 a, g dbSNP:55676763
1191 1191 g, t dbSNP:151340622
1249 1249 c, t dbSNP:387907402
1266 1266 c, t dbSNP:273585645
1323 1323 c, t dbSNP:151340626
1371 1371 g, t dbSNP:151340630
1407 1407 a, g dbSNP:273585644
1426 1426 c, g dbSNP:273585648
1429 1429 a, g dbSNP:151340620
1431 1431 a, c, g dbSNP:151340628
1612 1612 a, c dbSNP:273585646
1620 1620 a, g dbSNP:151340627
2109 2109 a, g dbSNP:151340625
2129 2129 a, g dbSNP:273585647
2150 2150 c, t dbSNP:151340623
2210 2210 a, g dbSNP:387907403
2229 2229 a, g dbSNP:273585649
2231 2231 g, t dbSNP:273585650
2366 2366 a, g dbSNP:387907404
2534 2534 a, c, t dbSNP:151340621
2702 2702 c, t dbSNP:151340624
3182 3182 a, g dbSNP:111438914
4283 4283 c, t dbSNP:12835125
4433 4433 a, g dbSNP:12859644
5284 5284 a, c dbSNP:12687955
5293 5293 a, g dbSNP:111452206
5382 5382 c, t dbSNP:111369735
5813 5813 a, g dbSNP:56147923
6088 6088 c, t dbSNP:112868450
6242 6242 a, g dbSNP:59126550
6485 6485 c, t dbSNP:56657044
7664 7664 g, t dbSNP:12845771
7666 7666 a, g dbSNP:12837391
7977 7977 c, t dbSNP:111560086
8996 8996 c, t dbSNP:41312128

Target ORF information:

RefSeq Version NM_001127898
Organism Homo sapiens (human)
Definition Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001127898

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCCATGT GGCAGGGTGC CATGGATAAC AGAGGCTTTC AGCAGGGGAG TTTTAGTAGC 
TTCCAGAACA GCTCCAGTGA TGAAGACCTG ATGGACATTC CAGCAACCGC TATGGATTTC
TCCATGAGAG ATGATGTTCC TCCCTTAGAC CGAGAAGTAG GAGAGGACAA GTCGTACAAT
GGTGGAGGAA TAGGTTCTTC AAATAGGATC ATGGACTTCT TGGAGGAGCC AATCCCTGGT
GTAGGGACCT ATGATGATTT CAATACAATT GATTGGGTGA GAGAGAAGTC TCGAGACCGG
GATAGGCACC GAGAGATTAC CAATAAAAGC AAAGAGTCAA CATGGGCCTT AATTCACAGT
GTGAGTGATG CTTTTTCCGG CTGGTTGTTG ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG
TTAGCTGGTT TGATAGACAT CTCTGCTCAT TGGATGACAG ACTTAAAAGA AGGTATATGC
ACAGGGGGAT TCTGGTTTAA CCATGAACAT TGTTGCTGGA ACTCTGAGCA TGTCACCTTT
GAAGAGAGAG ACAAATGTCC AGAGTGGAAT AGTTGGTCCC AGCTTATCAT CAGCACAGAT
GAGGGAGCCT TTGCCTACAT AGTCAATTAT TTCATGTACG TCCTCTGGGC TCTCCTATTT
GCCTTCCTTG CCGTATCTCT TGTCAAGGTG TTTGCGCCTT ATGCCTGTGG CTCTGGAATC
CCTGAGATAA AAACTATCTT GAGTGGTTTC ATTATTAGGG GCTATTTGGG TAAGTGGACT
CTGGTTATCA AAACCATCAC CTTGGTGCTG GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA
GAGGGCCCTC TAGTGCACGT GGCTTGCTGC TGTGGGAACA TCCTGTGCCA CTGCTTCAAC
AAATACAGGA AGAATGAAGC CAAGCGCAGA GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT
GTATCTGTAG CCTTTGGAGC ACCTATAGGT GGAGTATTAT TCAGCCTTGA AGAGGTCAGC
TACTATTTTC CCCTCAAAAC ATTGTGGCGT TCATTCTTTG CTGCCTTGGT GGCAGCATTC
ACTCTACGCT CCATCAATCC ATTTGGGAAC AGCCGCCTGG TACTATTTTA TGTGGAGTTT
CACACCCCAT GGCATCTCTT TGAGCTCGTG CCATTCATTC TGCTGGGCAT ATTTGGTGGT
CTGTGGGGAG CACTGTTTAT CCGCACAAAC ATTGCCTGGT GTCGGAAGCG AAAGACCACC
CAGTTGGGCA AGTATCCTGT TATAGAGGTA CTCGTCGTGA CAGCCATCAC TGCCATCCTG
GCTTTCCCCA ATGAATACAC TCGGATGAGC ACAAGTGAGC TCATTTCTGA GCTGTTTAAT
GACTGTGGCC TTCTGGACTC CTCCAAGCTC TGTGATTATG AGAACCGTTT CAACACAAGC
AAAGGGGGTG AACTGCCTGA CAGACCGGCT GGCGTGGGAG TCTACAGTGC AATGTGGCAG
CTGGCTTTAA CACTCATACT GAAAATTGTC ATTACTATAT TCACCTTTGG CATGAAGATC
CCTTCTGGCC TCTTTATCCC TAGCATGGCT GTTGGTGCTA TAGCAGGTCG ACTTCTAGGA
GTAGGAATGG AACAGCTGGC TTATTACCAC CAGGAATGGA CCGTCTTCAA TAGCTGGTGT
AGTCAGGGAG CTGATTGCAT CACCCCCGGC CTTTATGCAA TGGTTGGGGC TGCAGCCTGC
TTAGGTGGGG TGACTCGGAT GACTGTTTCT CTTGTTGTCA TAATGTTTGA ACTGACTGGT
GGCTTAGAAT ACATCGTGCC TCTGATGGCT GCAGCCATGA CAAGCAAGTG GGTGGCAGAT
GCTCTTGGGC GGGAGGGCAT CTATGATGCC CACATCCGTC TCAATGGATA CCCCTTTCTT
GAAGCCAAAG AAGAGTTTGC TCATAAGACC CTGGCAATGG ATGTGATGAA ACCCCGGAGA
AATGATCCTT TGTTGACTGT CCTTACTCAG GACAGTATGA CTGTGGAAGA TGTAGAGACC
ATAATCAGTG AAACCACTTA CAGTGGCTTC CCAGTGGTGG TATCCCGGGA GTCCCAAAGA
CTTGTGGGCT TTGTCCTCCG AAGAGATCTC ATTATTTCAA TTGAAAATGC TCGAAAGAAA
CAGGATGGGG TTGTTAGCAC TTCCATCATT TATTTCACGG AGCATTCTCC TCCATTGCCA
CCATACACTC CACCCACTCT AAAGCTTCGG AACATCCTCG ATCTCAGCCC CTTCACTGTG
ACTGACCTTA CACCCATGGA GATCGTAGTG GATATTTTCC GAAAGCTGGG ACTGCGGCAG
TGCCTGGTTA CACACAACGG GCGATTGCTT GGAATCATTA CCAAAAAGGA TGTGTTAAAG
CATATAGCAC AGATGGCGAA CCAAGATCCT GATTCCATTC TCTTCAACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21358
Accession Version NM_000084.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product H(+)/Cl(-) exchange transporter 5 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX950885.1, AK290940.1, AC231645.2, BC051691.1, AK025562.1 and AA743724.1. On Aug 21, 2013 this sequence version replaced gi:441703659. Summary: This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]. Transcript Variant: This variant (3, also known as type 3) lacks several 5' exons but has an alternate 5' exon, and it thus differs in the 5' UTR and uses a downstream start codon, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK308089.1, AK290940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGACTTCT TGGAGGAGCC AATCCCTGGT GTAGGGACCT ATGATGATTT CAATACAATT 
GATTGGGTGA GAGAGAAGTC TCGAGACCGG GATAGGCACC GAGAGATTAC CAATAAAAGC
AAAGAGTCAA CATGGGCCTT AATTCACAGT GTGAGTGATG CTTTTTCCGG CTGGTTGTTG
ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG TTAGCTGGTT TGATAGACAT CTCTGCTCAT
TGGATGACAG ACTTAAAAGA AGGTATATGC ACAGGGGGAT TCTGGTTTAA CCATGAACAT
TGTTGCTGGA ACTCTGAGCA TGTCACCTTT GAAGAGAGAG ACAAATGTCC AGAGTGGAAT
AGTTGGTCCC AGCTTATCAT CAGCACAGAT GAGGGAGCCT TTGCCTACAT AGTCAATTAT
TTCATGTACG TCCTCTGGGC TCTCCTATTT GCCTTCCTTG CCGTATCTCT TGTCAAGGTG
TTTGCGCCTT ATGCCTGTGG CTCTGGAATC CCTGAGATAA AAACTATCTT GAGTGGTTTC
ATTATTAGGG GCTATTTGGG TAAGTGGACT CTGGTTATCA AAACCATCAC CTTGGTGCTG
GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA GAGGGCCCTC TAGTGCACGT GGCTTGCTGC
TGTGGGAACA TCCTGTGCCA CTGCTTCAAC AAATACAGGA AGAATGAAGC CAAGCGCAGA
GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT GTATCTGTAG CCTTTGGAGC ACCTATAGGT
GGAGTATTAT TCAGCCTTGA AGAGGTCAGC TACTATTTTC CCCTCAAAAC ATTGTGGCGT
TCATTCTTTG CTGCCTTGGT GGCAGCATTC ACTCTACGCT CCATCAATCC ATTTGGGAAC
AGCCGCCTGG TACTATTTTA TGTGGAGTTT CACACCCCAT GGCATCTCTT TGAGCTCGTG
CCATTCATTC TGCTGGGCAT ATTTGGTGGT CTGTGGGGAG CACTGTTTAT CCGCACAAAC
ATTGCCTGGT GTCGGAAGCG AAAGACCACC CAGTTGGGCA AGTATCCTGT TATAGAGGTA
CTCGTCGTGA CAGCCATCAC TGCCATCCTG GCTTTCCCCA ATGAATACAC TCGGATGAGC
ACAAGTGAGC TCATTTCTGA GCTGTTTAAT GACTGTGGCC TTCTGGACTC CTCCAAGCTC
TGTGATTATG AGAACCGTTT CAACACAAGC AAAGGGGGTG AACTGCCTGA CAGACCGGCT
GGCGTGGGAG TCTACAGTGC AATGTGGCAG CTGGCTTTAA CACTCATACT GAAAATTGTC
ATTACTATAT TCACCTTTGG CATGAAGATC CCTTCTGGCC TCTTTATCCC TAGCATGGCT
GTTGGTGCTA TAGCAGGTCG ACTTCTAGGA GTAGGAATGG AACAGCTGGC TTATTACCAC
CAGGAATGGA CCGTCTTCAA TAGCTGGTGT AGTCAGGGAG CTGATTGCAT CACCCCCGGC
CTTTATGCAA TGGTTGGGGC TGCAGCCTGC TTAGGTGGGG TGACTCGGAT GACTGTTTCT
CTTGTTGTCA TAATGTTTGA ACTGACTGGT GGCTTAGAAT ACATCGTGCC TCTGATGGCT
GCAGCCATGA CAAGCAAGTG GGTGGCAGAT GCTCTTGGGC GGGAGGGCAT CTATGATGCC
CACATCCGTC TCAATGGATA CCCCTTTCTT GAAGCCAAAG AAGAGTTTGC TCATAAGACC
CTGGCAATGG ATGTGATGAA ACCCCGGAGA AATGATCCTT TGTTGACTGT CCTTACTCAG
GACAGTATGA CTGTGGAAGA TGTAGAGACC ATAATCAGTG AAACCACTTA CAGTGGCTTC
CCAGTGGTGG TATCCCGGGA GTCCCAAAGA CTTGTGGGCT TTGTCCTCCG AAGAGATCTC
ATTATTTCAA TTGAAAATGC TCGAAAGAAA CAGGATGGGG TTGTTAGCAC TTCCATCATT
TATTTCACGG AGCATTCTCC TCCATTGCCA CCATACACTC CACCCACTCT AAAGCTTCGG
AACATCCTCG ATCTCAGCCC CTTCACTGTG ACTGACCTTA CACCCATGGA GATCGTAGTG
GATATTTTCC GAAAGCTGGG ACTGCGGCAG TGCCTGGTTA CACACAACGG GCGATTGCTT
GGAATCATTA CCAAAAAGGA TGTGTTAAAG CATATAGCAC AGATGGCGAA CCAAGATCCT
GATTCCATTC TCTTCAACTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_000075.1
CDS249..2489
Misc Feature(1)135..137(+)
Misc Feature(2)411..524(+)
Misc Feature(3)462..1958(+)
Misc Feature(4)660..731(+)
Misc Feature(5)747..1922(+)
Misc Feature(6)747..761(+)
Misc Feature(7)804..863(+)
Misc Feature(8)873..887(+)
Misc Feature(9)879..938(+)
Misc Feature(10)879..881(+)
Misc Feature(11)879..881(+)
Misc Feature(12)1050..1052(+)
Misc Feature(13)1050..1052(+)
Misc Feature(14)1080..1136(+)
Misc Feature(15)1203..1283(+)
Misc Feature(16)1302..1364(+)
Misc Feature(17)1530..1592(+)
Misc Feature(18)1605..1664(+)
Misc Feature(19)1605..1619(+)
Misc Feature(20)1851..1904(+)
Misc Feature(21)1995..2435(+)
Misc Feature(22)2031..2441(+)
Exon (1)1..201
Gene:CLCN5
Gene Synonym:
Exon (2)202..353
Gene:CLCN5
Gene Synonym:
Exon (3)354..453
Gene:CLCN5
Gene Synonym:
Exon (4)454..641
Gene:CLCN5
Gene Synonym:
Exon (5)642..764
Gene:CLCN5
Gene Synonym:
Exon (6)765..971
Gene:CLCN5
Gene Synonym:
Exon (7)972..1052
Gene:CLCN5
Gene Synonym:
Exon (8)1053..1595
Gene:CLCN5
Gene Synonym:
Exon (9)1596..1782
Gene:CLCN5
Gene Synonym:
Exon (10)1783..2181
Gene:CLCN5
Gene Synonym:
Exon (11)2182..2398
Gene:CLCN5
Gene Synonym:
Exon (12)2399..9505
Gene:CLCN5
Gene Synonym:
Translation
Position Chain Variation Link
418 418 g, t dbSNP:151340629
455 455 c, t dbSNP:113780806
592 592 a, g dbSNP:55676763
847 847 g, t dbSNP:151340622
905 905 c, t dbSNP:387907402
922 922 c, t dbSNP:273585645
979 979 c, t dbSNP:151340626
1027 1027 g, t dbSNP:151340630
1063 1063 a, g dbSNP:273585644
1082 1082 c, g dbSNP:273585648
1085 1085 a, g dbSNP:151340620
1087 1087 a, c, g dbSNP:151340628
1268 1268 a, c dbSNP:273585646
1276 1276 a, g dbSNP:151340627
1765 1765 a, g dbSNP:151340625
1785 1785 a, g dbSNP:273585647
1806 1806 c, t dbSNP:151340623
1866 1866 a, g dbSNP:387907403
1885 1885 a, g dbSNP:273585649
1887 1887 g, t dbSNP:273585650
2022 2022 a, g dbSNP:387907404
2190 2190 a, c, t dbSNP:151340621
2358 2358 c, t dbSNP:151340624
2838 2838 a, g dbSNP:111438914
3939 3939 c, t dbSNP:12835125
4089 4089 a, g dbSNP:12859644
4940 4940 a, c dbSNP:12687955
4949 4949 a, g dbSNP:111452206
5038 5038 c, t dbSNP:111369735
5469 5469 a, g dbSNP:56147923
5744 5744 c, t dbSNP:112868450
5898 5898 a, g dbSNP:59126550
6141 6141 c, t dbSNP:56657044
7320 7320 g, t dbSNP:12845771
7322 7322 a, g dbSNP:12837391
7633 7633 c, t dbSNP:111560086
8652 8652 c, t dbSNP:41312128

Target ORF information:

RefSeq Version NM_000084
Organism Homo sapiens (human)
Definition Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000084

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGACTTCT TGGAGGAGCC AATCCCTGGT GTAGGGACCT ATGATGATTT CAATACAATT 
GATTGGGTGA GAGAGAAGTC TCGAGACCGG GATAGGCACC GAGAGATTAC CAATAAAAGC
AAAGAGTCAA CATGGGCCTT AATTCACAGT GTGAGTGATG CTTTTTCCGG CTGGTTGTTG
ATGCTCCTTA TTGGGCTTTT ATCAGGTTCG TTAGCTGGTT TGATAGACAT CTCTGCTCAT
TGGATGACAG ACTTAAAAGA AGGTATATGC ACAGGGGGAT TCTGGTTTAA CCATGAACAT
TGTTGCTGGA ACTCTGAGCA TGTCACCTTT GAAGAGAGAG ACAAATGTCC AGAGTGGAAT
AGTTGGTCCC AGCTTATCAT CAGCACAGAT GAGGGAGCCT TTGCCTACAT AGTCAATTAT
TTCATGTACG TCCTCTGGGC TCTCCTATTT GCCTTCCTTG CCGTATCTCT TGTCAAGGTG
TTTGCGCCTT ATGCCTGTGG CTCTGGAATC CCTGAGATAA AAACTATCTT GAGTGGTTTC
ATTATTAGGG GCTATTTGGG TAAGTGGACT CTGGTTATCA AAACCATCAC CTTGGTGCTG
GCAGTGTCAT CTGGCTTGAG CCTGGGCAAA GAGGGCCCTC TAGTGCACGT GGCTTGCTGC
TGTGGGAACA TCCTGTGCCA CTGCTTCAAC AAATACAGGA AGAATGAAGC CAAGCGCAGA
GAGGTCTTGT CGGCTGCAGC AGCAGCTGGT GTATCTGTAG CCTTTGGAGC ACCTATAGGT
GGAGTATTAT TCAGCCTTGA AGAGGTCAGC TACTATTTTC CCCTCAAAAC ATTGTGGCGT
TCATTCTTTG CTGCCTTGGT GGCAGCATTC ACTCTACGCT CCATCAATCC ATTTGGGAAC
AGCCGCCTGG TACTATTTTA TGTGGAGTTT CACACCCCAT GGCATCTCTT TGAGCTCGTG
CCATTCATTC TGCTGGGCAT ATTTGGTGGT CTGTGGGGAG CACTGTTTAT CCGCACAAAC
ATTGCCTGGT GTCGGAAGCG AAAGACCACC CAGTTGGGCA AGTATCCTGT TATAGAGGTA
CTCGTCGTGA CAGCCATCAC TGCCATCCTG GCTTTCCCCA ATGAATACAC TCGGATGAGC
ACAAGTGAGC TCATTTCTGA GCTGTTTAAT GACTGTGGCC TTCTGGACTC CTCCAAGCTC
TGTGATTATG AGAACCGTTT CAACACAAGC AAAGGGGGTG AACTGCCTGA CAGACCGGCT
GGCGTGGGAG TCTACAGTGC AATGTGGCAG CTGGCTTTAA CACTCATACT GAAAATTGTC
ATTACTATAT TCACCTTTGG CATGAAGATC CCTTCTGGCC TCTTTATCCC TAGCATGGCT
GTTGGTGCTA TAGCAGGTCG ACTTCTAGGA GTAGGAATGG AACAGCTGGC TTATTACCAC
CAGGAATGGA CCGTCTTCAA TAGCTGGTGT AGTCAGGGAG CTGATTGCAT CACCCCCGGC
CTTTATGCAA TGGTTGGGGC TGCAGCCTGC TTAGGTGGGG TGACTCGGAT GACTGTTTCT
CTTGTTGTCA TAATGTTTGA ACTGACTGGT GGCTTAGAAT ACATCGTGCC TCTGATGGCT
GCAGCCATGA CAAGCAAGTG GGTGGCAGAT GCTCTTGGGC GGGAGGGCAT CTATGATGCC
CACATCCGTC TCAATGGATA CCCCTTTCTT GAAGCCAAAG AAGAGTTTGC TCATAAGACC
CTGGCAATGG ATGTGATGAA ACCCCGGAGA AATGATCCTT TGTTGACTGT CCTTACTCAG
GACAGTATGA CTGTGGAAGA TGTAGAGACC ATAATCAGTG AAACCACTTA CAGTGGCTTC
CCAGTGGTGG TATCCCGGGA GTCCCAAAGA CTTGTGGGCT TTGTCCTCCG AAGAGATCTC
ATTATTTCAA TTGAAAATGC TCGAAAGAAA CAGGATGGGG TTGTTAGCAC TTCCATCATT
TATTTCACGG AGCATTCTCC TCCATTGCCA CCATACACTC CACCCACTCT AAAGCTTCGG
AACATCCTCG ATCTCAGCCC CTTCACTGTG ACTGACCTTA CACCCATGGA GATCGTAGTG
GATATTTTCC GAAAGCTGGG ACTGCGGCAG TGCCTGGTTA CACACAACGG GCGATTGCTT
GGAATCATTA CCAAAAAGGA TGTGTTAAAG CATATAGCAC AGATGGCGAA CCAAGATCCT
GATTCCATTC TCTTCAACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16814
Accession Version NM_001272102.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 195bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product H(+)/Cl(-) exchange transporter 5 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092049.1 and BF513571.1. Summary: This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]. Transcript Variant: This variant (5) uses an alternate splice site in the 5' UTR, it lacks several 3' exons but has an alternate 3' terminal exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) has a distinct and shorter C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB014315.1, DA879207.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGGCCATGT GGCAGGGTGC CATGGATAAC AGAGGCTTTC AGCAGGGGAG TTTTAGTAGC 
TTCCAGAACA GCTCCAGTGA TGAAGACCTG ATGGACATTC CAGCAACCGC TATGGATTTC
TCCATGAGAG ATGATGTTCC TCCCTTAGAC CGAGAAGTAG GAGGTATCAT TATTGGTGAT
GATAATTTAT CTTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001259031.1
CDS383..577
Misc Feature(1)326..328(+)
Exon (1)1..178
Gene:CLCN5
Gene Synonym:
Exon (2)179..254
Gene:CLCN5
Gene Synonym:
Exon (3)255..398
Gene:CLCN5
Gene Synonym:
Exon (4)399..624
Gene:CLCN5
Gene Synonym:
Translation
Position Chain Variation Link
41 41 -, c dbSNP:142374236
490 490 c, t dbSNP:111464924

Target ORF information:

RefSeq Version NM_001272102
Organism Homo sapiens (human)
Definition Homo sapiens chloride channel, voltage-sensitive 5 (CLCN5), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001272102

ORF Insert Sequence:

1
61
121
181
ATGGCCATGT GGCAGGGTGC CATGGATAAC AGAGGCTTTC AGCAGGGGAG TTTTAGTAGC 
TTCCAGAACA GCTCCAGTGA TGAAGACCTG ATGGACATTC CAGCAACCGC TATGGATTTC
TCCATGAGAG ATGATGTTCC TCCCTTAGAC CGAGAAGTAG GAGGTATCAT TATTGGTGAT
GATAATTTAT CTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.