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HIST2H3C cDNA ORF clone, Homo sapiens (human)

Gene Symbol HIST2H3C
Entrez Gene ID 126961
Full Name histone cluster 2, H3c
Synonyms H3, H3.2, H3/M, H3F2, H3FM, H3FN
General protein information
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q21.2

Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

142780

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_021059 NP_066403 histone H3.2

KEGG
hsa05322 Systemic lupus erythematosus
hsa05202 Transcriptional misregulation in cancer
hsa05034 Alcoholism
Reactome
R-HSA-1643685 Disease
R-HSA-977225 Amyloid formation
R-HSA-109582 Hemostasis
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
R-HSA-162582 Signal Transduction
R-HSA-195258 RHO GTPase Effectors
R-HSA-5625740 RHO GTPases activate PKNs
R-HSA-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-HSA-194315 Signaling by Rho GTPases
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-74160 Gene Expression
R-HSA-195721 Signaling by Wnt
R-HSA-73728 RNA Polymerase I Promoter Opening
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-504046 RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
R-HSA-73854 RNA Polymerase I Promoter Clearance
R-HSA-73864 RNA Polymerase I Transcription
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-211000 Gene Silencing by RNA
R-HSA-212165 Epigenetic regulation of gene expression
R-HSA-5250941 Negative epigenetic regulation of rRNA expression
R-HSA-427359 SIRT1 negatively regulates rRNA Expression
R-HSA-5334118 DNA methylation
R-HSA-69278 Cell Cycle, Mitotic
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-1640170 Cell Cycle
R-HSA-68875 Mitotic Prophase
R-HSA-2299718 Condensation of Prophase Chromosomes
R-HSA-68886 M Phase
R-HSA-212300 PRC2 methylates histones and DNA
R-HSA-1500620 Meiosis
R-HSA-912446 Meiotic recombination
R-HSA-4839726 Chromatin organization
R-HSA-3214847 HATs acetylate histones
R-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-2262752 Cellular responses to stress
R-HSA-3247509 Chromatin modifying enzymes
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-2559583 Cellular Senescence
R-HSA-3214842 HDMs demethylate histones
R-HSA-3214841 PKMTs methylate histone lysines
R-HSA-3214815 HDACs deacetylate histones
R-HSA-3214858 RMTs methylate histone arginines
WikiPathways
WP395 IL-4 signaling Pathway


Homo sapiens (human) HIST2H3C NP_066403.2
Pan troglodytes (chimpanzee) LOC469476 XP_524859.2
Macaca mulatta (Rhesus monkey) LOC710748 XP_001109042.1
Canis lupus familiaris (dog) LOC100856221 XP_003638893.1
Bos taurus (cattle) LOC781224 XP_005208273.1
Mus musculus (house mouse) H3f3c XP_894986.1
Rattus norvegicus (Norway rat) H3f3c XP_003750396.1
Drosophila melanogaster (fruit fly) His3:CG33824 NP_001027318.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100486720 XP_002944964.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HIST2H3C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST2H3C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu13909
NM_021059 Homo sapiens histone cluster 2, H3c (HIST2H3C), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OHu13909
Accession Version NM_021059.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 411bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 18-AUG-2015
Organism Homo sapiens (human)
Product histone H3.2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF531307.1. On or before Sep 20, 2006 this sequence version replaced gi:113431186, gi:22748792, gi:19743832. Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Aug 2015]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGGCCCGTA CTAAGCAGAC TGCTCGCAAG TCGACCGGCG GCAAGGCCCC GAGGAAGCAG 
CTGGCCACCA AGGCGGCCCG CAAGAGCGCG CCGGCCACGG GCGGGGTGAA GAAGCCGCAC
CGCTACCGGC CCGGCACCGT AGCCCTGCGG GAGATCCGGC GCTACCAGAA GTCCACGGAG
CTGCTGATCC GCAAGCTGCC CTTCCAGCGG CTGGTACGCG AGATCGCGCA GGACTTTAAG
ACGGACCTGC GCTTCCAGAG CTCGGCCGTG ATGGCGCTGC AGGAGGCCAG CGAGGCCTAC
CTGGTGGGGC TGTTCGAAGA CACGAACCTG TGCGCCATCC ACGCCAAGCG CGTGACCATT
ATGCCCAAGG ACATCCAGCT GGCCCGCCGC ATCCGTGGAG AGCGGGCTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_066403.2
CDS37..447
Misc Feature(1)37..444(+)
Misc Feature(2)37..435(+)
Misc Feature(3)43..45(+)
Misc Feature(4)43..45(+)
Misc Feature(5)46..48(+)
Misc Feature(6)49..51(+)
Misc Feature(7)49..51(+)
Misc Feature(8)49..51(+)
Misc Feature(9)49..51(+)
Misc Feature(10)55..57(+)
Misc Feature(11)61..63(+)
Misc Feature(12)61..63(+)
Misc Feature(13)64..66(+)
Misc Feature(14)64..66(+)
Misc Feature(15)64..66(+)
Misc Feature(16)67..69(+)
Misc Feature(17)70..72(+)
Misc Feature(18)79..81(+)
Misc Feature(19)88..90(+)
Misc Feature(20)88..90(+)
Misc Feature(21)91..93(+)
Misc Feature(22)91..93(+)
Misc Feature(23)91..93(+)
Misc Feature(24)106..108(+)
Misc Feature(25)106..108(+)
Misc Feature(26)106..108(+)
Misc Feature(27)115..117(+)
Misc Feature(28)118..120(+)
Misc Feature(29)118..120(+)
Misc Feature(30)118..120(+)
Misc Feature(31)121..123(+)
Misc Feature(32)142..144(+)
Misc Feature(33)145..147(+)
Misc Feature(34)145..147(+)
Misc Feature(35)148..150(+)
Misc Feature(36)160..162(+)
Misc Feature(37)205..207(+)
Misc Feature(38)205..207(+)
Misc Feature(39)205..207(+)
Misc Feature(40)208..210(+)
Misc Feature(41)229..231(+)
Misc Feature(42)274..276(+)
Misc Feature(43)274..276(+)
Misc Feature(44)277..279(+)
Misc Feature(45)295..297(+)
Misc Feature(46)358..360(+)
Misc Feature(47)382..384(+)
Misc Feature(48)403..405(+)
Misc Feature(49)403..405(+)
Exon (1)1..507
Gene:HIST2H3C
Gene Synonym:
Translation
Position Chain Variation Link
201 201 c, t dbSNP:584378
234 234 a, g dbSNP:7524039
308 308 c, t dbSNP:2664732
327 327 a, c dbSNP:584902
351 351 c, t dbSNP:58712756

Target ORF information:

RefSeq Version NM_021059
Organism Homo sapiens (human)
Definition Homo sapiens histone cluster 2, H3c (HIST2H3C), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021059

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGCCCGTA CTAAGCAGAC TGCTCGCAAG TCGACCGGCG GCAAGGCCCC GAGGAAGCAG 
CTGGCCACCA AGGCGGCCCG CAAGAGCGCG CCGGCCACGG GCGGGGTGAA GAAGCCGCAC
CGCTACCGGC CCGGCACCGT AGCCCTGCGG GAGATCCGGC GCTACCAGAA GTCCACGGAG
CTGCTGATCC GCAAGCTGCC CTTCCAGCGG CTGGTACGCG AGATCGCGCA GGACTTTAAG
ACGGACCTGC GCTTCCAGAG CTCGGCCGTG ATGGCGCTGC AGGAGGCCAG CGAGGCCTAC
CTGGTGGGGC TGTTCGAAGA CACGAACCTG TGCGCCATCC ACGCCAAGCG CGTGACCATT
ATGCCCAAGG ACATCCAGCT GGCCCGCCGC ATCCGTGGAG AGCGGGCTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Human tNASP promotes in vitro nucleosome assembly with histone H3.3
Biochemistry 54 (5), 1171-1179 (2015)
Kato D, Osakabe A, Tachiwana H, Tanaka H and Kurumizaka H.


book

Mitotic phosphorylation of histone H3 threonine 80
Cell Cycle 13 (3), 440-452 (2014)
Hammond SL, Byrum SD, Namjoshi S, Graves HK, Dennehey BK, Tackett AJ and Tyler JK.


book

Symmetric dimethylation of H3R2 is a newly identified histone mark that supports euchromatin maintenance
Nat. Struct. Mol. Biol. 19 (2), 136-144 (2012)
Migliori V, Muller J, Phalke S, Low D, Bezzi M, Mok WC, Sahu SK, Gunaratne J, Capasso P, Bassi C, Cecatiello V, De Marco A, Blackstock W, Kuznetsov V, Amati B, Mapelli M and Guccione E.


book

UHRF1 double tudor domain and the adjacent PHD finger act together to recognize K9me3-containing histone H3 tail
J. Mol. Biol. 415 (2), 318-328 (2012)
Xie S, Jakoncic J and Qian C.


book

A specific function for the histone chaperone NASP to fine-tune a reservoir of soluble H3-H4 in the histone supply chain
Mol. Cell 44 (6), 918-927 (2011)
Cook AJ, Gurard-Levin ZA, Vassias I and Almouzni G.


book

The human and mouse replication-dependent histone genes
Genomics 80 (5), 487-498 (2002)
Marzluff WF, Gongidi P, Woods KR, Jin J and Maltais LJ.


book

Prothymosin alpha binds histones in vitro and shows activity in nucleosome assembly assay
Biochim. Biophys. Acta 1296 (2), 219-227 (1996)
Diaz-Jullien C, Perez-Estevez A, Covelo G and Freire M.


book

Enhancer-facilitated expression of prokaryotic and eukaryotic genes using human histone gene 5' regulatory sequences
Biochem. Cell Biol. 64 (4), 277-289 (1986)
Marashi,F., Helms,S., Shiels,A., Silverstein,S., Greenspan,D.S., Stein,G. and Stein,J.


book

A major human histone gene cluster on the long arm of chromosome 1
Science 226 (4676), 838-840 (1984)
Green,L., Van Antwerpen,R., Stein,J., Stein,G., Tripputi,P., Emanuel,B., Selden,J. and Croce,C.


book

Human spleen histone H3. Isolation and amino acid sequence
J. Biochem. 90 (4), 1205-1211 (1981)
Ohe,Y. and Iwai,K.


 
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