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EDARADD cDNA ORF clone, Homo sapiens (human)

Gene Symbol EDARADD
Entrez Gene ID 128178
Full Name EDAR-associated death domain
Synonyms ECTD11A, ECTD11B, ED3, EDA3
General protein information
Preferred Names
ectodysplasin-A receptor-associated adapter protein
Names
ectodysplasin-A receptor-associated adapter protein
crinkled homolog
EDAR-associated death domain protein
ectodysplasia A receptor associated death domain
ectodysplasin A receptor associated adapter protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q42.3

Summary This gene was identified by its association with ectodermal dysplasia, a genetic disorder characterized by defective development of hair, teeth, and eccrine sweat glands. The protein encoded by this gene is a death domain-containing protein, and is found to interact with EDAR, a death domain receptor known to be required for the development of hair, teeth and other ectodermal derivatives. This protein and EDAR are coexpressed in epithelial cells during the formation of hair follicles and teeth. Through its interaction with EDAR, this protein acts as an adaptor, and links the receptor to downstream signaling pathways. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

606603

Disorder Html: Ectodermal dysplasia, anhidrotic, autosomal recessive, 224900 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_145861 NP_665860 ectodysplasin-A receptor-associated adapter protein isoform A
NM_080738 NP_542776 ectodysplasin-A receptor-associated adapter protein isoform B



Homo sapiens (human) EDARADD NP_665860.2
Macaca mulatta (Rhesus monkey) EDARADD XP_001099801.1
Canis lupus familiaris (dog) EDARADD XP_005618853.1
Bos taurus (cattle) EDARADD XP_002698858.2
Mus musculus (house mouse) Edaradd NP_598398.3
Rattus norvegicus (Norway rat) Edaradd XP_574055.4
Gallus gallus (chicken) EDARADD NP_001012405.2
Danio rerio (zebrafish) edaradd XP_695138.4
Xenopus (Silurana) tropicalis (western clawed frog) LOC100490133 XP_002933922.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EDARADD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDARADD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu19463 NM_145861 Homo sapiens EDAR-associated death domain (EDARADD), transcript variant A, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $188.30
$269.00
OHu16488 NM_080738 Homo sapiens EDAR-associated death domain (EDARADD), transcript variant B, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $188.30
$269.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19463
Clone ID Related Accession (Same CDS sequence) NM_145861
Accession Version NM_145861.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 648bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ectodysplasin-A receptor-associated adapter protein isoform A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354693.6, AY028913.1 and AL136105.9. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2007 this sequence version replaced gi:22325374. Transcript Variant: This variant (A) encodes a longer isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY028913.1, BC128082.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

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121
181
241
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361
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481
541
601
ATGGGCCTCA GGACGACTAA ACAGATGGGG AGAGGCACTA AAGCTCCTGG TCACCAAGAG 
GATCATATGG TAAAGGAACC AGTGGAAGAC ACAGACCCTA GCACTTTATC CTTTAATATG
TCAGACAAAT ATCCCATTCA AGATACGGAA CTCCCTAAAG CTGAAGAATG TGATACAATT
ACTTTGAACT GCCCACGAAA TTCAGATATG AAAAATCAGG GAGAAGAAAA TGGCTTTCCA
GATAGCACTG GAGATCCTCT TCCAGAGATC AGCAAGGACA ACTCCTGCAA AGAAAACTGT
ACTTGTTCCT CCTGCTTGCT CCGGGCCCCC ACCATAAGTG ACTTGCTCAA TGATCAGGAC
TTACTAGACG TGATCAGGAT AAAGCTGGAT CCGTGTCACC CAACGGTGAA AAACTGGAGG
AATTTTGCAA GCAAATGGGG GATGTCCTAT GACGAATTGT GCTTCCTGGA GCAGAGGCCA
CAGAGCCCCA CCTTGGAGTT CTTGCTCCGG AACAGTCAGA GGACGGTGGG CCAGCTGATG
GAGCTCTGCA GGCTCTACCA CAGGGCCGAC GTGGAGAAGG TTCTGCGCAG GTGGGTGGAC
GAGGAGTGGC CCAAGCGGGA GCGTGGAGAC CCCTCCAGGC ACTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_665860.2
CDS66..713
Misc Feature(1)42..44(+)
Misc Feature(2)459..662(+)
Exon (1)1..126
Gene:EDARADD
Gene Synonym:
Exon (2)127..185
Gene:EDARADD
Gene Synonym:
Exon (3)186..225
Gene:EDARADD
Gene Synonym:
Exon (4)226..284
Gene:EDARADD
Gene Synonym:
Exon (5)285..330
Gene:EDARADD
Gene Synonym:
Exon (6)331..2772
Gene:EDARADD
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_145861
Organism Homo sapiens (human)
Definition Homo sapiens EDAR-associated death domain (EDARADD), transcript variant A, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_145861

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGGCCTCA GGACGACTAA ACAGATGGGG AGAGGCACTA AAGCTCCTGG TCACCAAGAG 
GATCATATGG TAAAGGAACC AGTGGAAGAC ACAGACCCTA GCACTTTATC CTTTAATATG
TCAGACAAAT ATCCCATTCA AGATACGGAA CTCCCTAAAG CTGAAGAATG TGATACAATT
ACTTTGAACT GCCCACGAAA TTCAGATATG AAAAATCAGG GAGAAGAAAA TGGCTTTCCA
GATAGCACTG GAGATCCTCT TCCAGAGATC AGCAAGGACA ACTCCTGCAA AGAAAACTGT
ACTTGTTCCT CCTGCTTGCT CCGGGCCCCC ACCATAAGTG ACTTGCTCAA TGATCAGGAC
TTACTAGACG TGATCAGGAT AAAGCTGGAT CCGTGTCACC CAACGGTGAA AAACTGGAGG
AATTTTGCAA GCAAATGGGG GATGTCCTAT GACGAATTGT GCTTCCTGGA GCAGAGGCCA
CAGAGCCCCA CCTTGGAGTT CTTGCTCCGG AACAGTCAGA GGACGGTGGG CCAGCTGATG
GAGCTCTGCA GGCTCTACCA CAGGGCCGAC GTGGAGAAGG TTCTGCGCAG GTGGGTGGAC
GAGGAGTGGC CCAAGCGGGA GCGTGGAGAC CCCTCCAGGC ACTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16488
Clone ID Related Accession (Same CDS sequence) NM_080738
Accession Version NM_080738.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product ectodysplasin-A receptor-associated adapter protein isoform B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096339.1 and BC006533.2. On Jul 24, 2007 this sequence version replaced gi:22325373. Transcript Variant: This variant (B) differs in the 5' region compared to variant A. It encodes an isoform (B) that contains a distinct and shorter N-terminus, as compared to isoform (A). ##Evidence-Data-START## Transcript exon combination :: AK096339.1, BC006533.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

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241
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361
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481
541
601
ATGGCTTCAC CGGACGACCC TCTGCGCGCA GATCATATGG TAAAGGAACC AGTGGAAGAC 
ACAGACCCTA GCACTTTATC CTTTAATATG TCAGACAAAT ATCCCATTCA AGATACGGAA
CTCCCTAAAG CTGAAGAATG TGATACAATT ACTTTGAACT GCCCACGAAA TTCAGATATG
AAAAATCAGG GAGAAGAAAA TGGCTTTCCA GATAGCACTG GAGATCCTCT TCCAGAGATC
AGCAAGGACA ACTCCTGCAA AGAAAACTGT ACTTGTTCCT CCTGCTTGCT CCGGGCCCCC
ACCATAAGTG ACTTGCTCAA TGATCAGGAC TTACTAGACG TGATCAGGAT AAAGCTGGAT
CCGTGTCACC CAACGGTGAA AAACTGGAGG AATTTTGCAA GCAAATGGGG GATGTCCTAT
GACGAATTGT GCTTCCTGGA GCAGAGGCCA CAGAGCCCCA CCTTGGAGTT CTTGCTCCGG
AACAGTCAGA GGACGGTGGG CCAGCTGATG GAGCTCTGCA GGCTCTACCA CAGGGCCGAC
GTGGAGAAGG TTCTGCGCAG GTGGGTGGAC GAGGAGTGGC CCAAGCGGGA GCGTGGAGAC
CCCTCCAGGC ACTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_542776.1
CDS215..832
Misc Feature(1)203..205(+)
Misc Feature(2)578..781(+)
Exon (1)1..245
Gene:EDARADD
Gene Synonym:
Exon (2)246..304
Gene:EDARADD
Gene Synonym:
Exon (3)305..344
Gene:EDARADD
Gene Synonym:
Exon (4)345..403
Gene:EDARADD
Gene Synonym:
Exon (5)404..449
Gene:EDARADD
Gene Synonym:
Exon (6)450..2891
Gene:EDARADD
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_080738
Organism Homo sapiens (human)
Definition Homo sapiens EDAR-associated death domain (EDARADD), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_080738

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCTTCAC CGGACGACCC TCTGCGCGCA GATCATATGG TAAAGGAACC AGTGGAAGAC 
ACAGACCCTA GCACTTTATC CTTTAATATG TCAGACAAAT ATCCCATTCA AGATACGGAA
CTCCCTAAAG CTGAAGAATG TGATACAATT ACTTTGAACT GCCCACGAAA TTCAGATATG
AAAAATCAGG GAGAAGAAAA TGGCTTTCCA GATAGCACTG GAGATCCTCT TCCAGAGATC
AGCAAGGACA ACTCCTGCAA AGAAAACTGT ACTTGTTCCT CCTGCTTGCT CCGGGCCCCC
ACCATAAGTG ACTTGCTCAA TGATCAGGAC TTACTAGACG TGATCAGGAT AAAGCTGGAT
CCGTGTCACC CAACGGTGAA AAACTGGAGG AATTTTGCAA GCAAATGGGG GATGTCCTAT
GACGAATTGT GCTTCCTGGA GCAGAGGCCA CAGAGCCCCA CCTTGGAGTT CTTGCTCCGG
AACAGTCAGA GGACGGTGGG CCAGCTGATG GAGCTCTGCA GGCTCTACCA CAGGGCCGAC
GTGGAGAAGG TTCTGCGCAG GTGGGTGGAC GAGGAGTGGC CCAAGCGGGA GCGTGGAGAC
CCCTCCAGGC ACTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A case-control study of the association between tooth-development gene polymorphisms and non-syndromic hypodontia in the Chinese Han population
Eur. J. Oral Sci. 120 (5), 378-385 (2012)
Liu H, Zhang J, Song S, Zhao H, Han D and Feng H.


book

Isolated oligodontia associated with mutations in EDARADD, AXIN2, MSX1, and PAX9 genes
Am. J. Med. Genet. A 155A (7), 1616-1622 (2011)
Bergendal B, Klar J, Stecksen-Blicks C, Norderyd J and Dahl N.


book

Only four genes (EDA1, EDAR, EDARADD, and WNT10A) account for 90% of hypohidrotic/anhidrotic ectodermal dysplasia cases
Hum. Mutat. 32 (1), 70-72 (2011)
Cluzeau C, Hadj-Rabia S, Jambou M, Mansour S, Guigue P, Masmoudi S, Bal E, Chassaing N, Vincent MC, Viot G, Clauss F, Maniere MC, Toupenay S, Le Merrer M, Lyonnet S, Cormier-Daire V, Amiel J, Faivre L, de Prost Y, Munnich A, Bonnefont JP, Bodemer C and Smahi A.


book

Mutations in EDARADD account for a small proportion of hypohidrotic ectodermal dysplasia cases
Br. J. Dermatol. 162 (5), 1044-1048 (2010)
Chassaing N, Cluzeau C, Bal E, Guigue P, Vincent MC, Viot G, Ginisty D, Munnich A, Smahi A and Calvas P.


book

Death receptor signaling giving life to ectodermal organs
Sci. STKE 2002 (131), PE22 (2002)
Thesleff I and Mikkola ML.


book

Identification of a novel death domain-containing adaptor molecule for ectodysplasin-A receptor that is mutated in crinkled mice
Curr. Biol. 12 (5), 409-413 (2002)
Yan M, Zhang Z, Brady JR, Schilbach S, Fairbrother WJ and Dixit VM.


book

Gene defect in ectodermal dysplasia implicates a death domain adapter in development
Nature 414 (6866), 913-916 (2001)
Headon DJ, Emmal SA, Ferguson BM, Tucker AS, Justice MJ, Sharpe PT, Zonana J and Overbeek PA.


book

The ectodermal dysplasia receptor activates the nuclear factor-kappaB, JNK, and cell death pathways and binds to ectodysplasin A
J. Biol. Chem. 276 (4), 2668-2677 (2001)
Kumar A, Eby MT, Sinha S, Jasmin A and Chaudhary PM.


book

Definitive evidence for an autosomal recessive form of hypohidrotic ectodermal dysplasia clinically indistinguishable from the more common X-linked disorder
Am. J. Hum. Genet. 61 (1), 94-100 (1997)
Munoz F, Lestringant G, Sybert V, Frydman M, Alswaini A, Frossard PM, Jorgenson R and Zonana J.


book

Hypohidrotic Ectodermal Dysplasia
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Wright,J.T., Grange,D.K. and Richter,M.K.


 
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