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COL4A3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol COL4A3
Entrez Gene ID 1285
Full Name collagen, type IV, alpha 3 (Goodpasture antigen)
General protein information
Preferred Names
collagen alpha-3(IV) chain
Names
collagen alpha-3(IV) chain
tumstatin
collagen IV, alpha-3 polypeptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q36-q37

Summary Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]. lac of sum
Disorder MIM:

120070

Disorder Html: Alport syndrome, autosomal recessive, 203780 (3); Hematuria,

mRNA and Protein(s)

mRNA Protein Name
XM_005246276 XP_005246333 collagen alpha-3(IV) chain isoform X1
XM_005246277 XP_005246334 collagen alpha-3(IV) chain isoform X2
XM_011510555 XP_011508857 collagen alpha-3(IV) chain isoform X3
XM_011510556 XP_011508858 collagen alpha-3(IV) chain isoform X4
XM_006712245 XP_006712308 collagen alpha-3(IV) chain isoform X5
XM_005246280 XP_005246337 collagen alpha-3(IV) chain isoform X6
NM_000091 NP_000082 collagen alpha-3(IV) chain precursor



Homo sapiens (human) COL4A3 NP_000082.2
Pan troglodytes (chimpanzee) COL4A3 XP_516130.2
Macaca mulatta (Rhesus monkey) COL4A3 XP_001101448.2
Canis lupus familiaris (dog) COL4A3 XP_005635825.1
Bos taurus (cattle) COL4A3 NP_001160001.1
Mus musculus (house mouse) Col4a3 NP_031760.2
Rattus norvegicus (Norway rat) Col4a3 NP_001129231.1
Gallus gallus (chicken) COL4A3 XP_001234730.3


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following COL4A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu35827 XM_005246276 PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $811.30
$1159.00
OHu35828 XM_005246277 PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $811.30
$1159.00
OHu56969 XM_011510555 PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $811.30
$1159.00
OHu56970 XM_011510556 PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu35830 XM_006712245 PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu35832 XM_005246280 PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu23179 NM_000091 Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu35827
Clone ID Related Accession (Same CDS sequence) XM_005246276
Accession Version XM_005246276.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4914bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-3(IV) chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530369762. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGTATTCA TGGTCTCCAG GGAGATAAGG GAGAGCCAGG TTATTCAGAA
GGTACAAGGC CAGGACCACC GGGACCAACG GGGGATCCAG GACTGCCGGG TGATATGGGA
AAGAAAGGAG AAATGGGGCA ACCTGGCCCA CCTGGACATT TGGGGCCTGC TGGACCTGAG
GGAGCCCCTG GAAGTCCTGG AAGTCCTGGC CTCCCAGGAA AGCCAGGTCC TCATGGTGAT
TTGGGTTTTA AAGGAATCAA AGGCCTCCTG GGCCCTCCAG GAATCAGAGG CCCTCCAGGT
CTTCCAGGAT TTCCAGGATC TCCTGGACCA ATGGGTATAA GAGGTGACCA AGGACGTGAT
GGAATTCCTG GTCCAGCCGG AGAAAAGGGA GAAACGGGTT TATTGAGGGC CCCTCCAGGC
CCAAGAGGGA ACCCTGGTGC TCAAGGAGCC AAAGGAGACA GGGGAGCCCC AGGTTTTCCT
GGCCTCCCGG GCAGAAAAGG GGCCATGGGA GATGCTGGAC CTCGAGGACC CACAGGCATA
GAAGGATTCC CAGGGCCACC AGGTCTGCCC GGTGCAATTA TCCCTGGCCA GACAGGAAAT
CGTGGTCCAC CAGGCTCAAG AGGAAGCCCA GGTGCGCCTG GTCCCCCTGG ACCTCCAGGG
AGTCATGTAA TAGGCATAAA AGGAGACAAA GGGTCTATGG GCCACCCTGG CCCAAAAGGT
CCACCTGGAA CTGCAGGAGA CATGGGACCA CCAGGTCGTC TGGGAGCACC AGGTACTCCA
GGTCTTCCAG GACCCAGAGG TGATCCTGGA TTCCAGGGGT TTCCAGGCGT GAAAGGAGAA
AAGGGTAATC CTGGATTTCT AGGATCCATT GGACCTCCAG GACCAATTGG GCCAAAAGGA
CCACCTGGTG TACGTGGAGA CCCTGGCACA CTTAAGATTA TCTCCCTTCC AGGAAGCCCA
GGGCCACCTG GCACACCTGG AGAACCAGGG ATGCAGGGAG AACCTGGGCC ACCAGGGCCA
CCTGGAAACC TAGGACCCTG TGGGCCAAGA GGTAAGCCAG GCAAGGATGG AAAACCAGGA
ACTCCTGGAC CAGCTGGAGA AAAAGGCAAC AAAGGTTCTA AAGGAGAGCC AGGACCAGCT
GGATCAGATG GATTGCCAGG TTTGAAAGGA AAACGTGGAG ACAGTGGATC ACCTGCAACC
TGGACAACGA GAGGCTTTGT CTTCACCCGA CACAGTCAAA CCACAGCAAT TCCTTCATGT
CCAGAGGGGA CAGTGCCACT CTACAGTGGG TTTTCTTTTC TTTTTGTACA AGGAAATCAA
CGAGCCCACG GACAAGACCT TGGAACTCTT GGCAGCTGCC TGCAGCGATT TACCACAATG
CCATTCTTAT TCTGCAATGT CAATGATGTA TGTAATTTTG CATCTCGAAA TGATTATTCA
TACTGGCTGT CAACACCAGC TCTGATGCCA ATGAACATGG CTCCCATTAC TGGCAGAGCC
CTTGAGCCTT ATATAAGCAG ATGCACTGTT TGTGAAGGTC CTGCGATCGC CATAGCCGTT
CACAGCCAAA CCACTGACAT TCCTCCATGT CCTCACGGCT GGATTTCTCT CTGGAAAGGA
TTTTCATTCA TCATGAAAGC CTATTCCATC AACTGTGAAA GCTGGGGAAT TAGAAAAAAT
AATAAGTCGC TGTCAGGTGT GCATGAAGAA AAGACACTGA AGCTAAAAAA GACAGCAGAA
CTGCTATTTT TCATCCTAAA GAACAAAGTA ATGACAGAAC ATGCTGTTAT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005246333.1
CDS139..5052
Misc Feature(1)337..510(+)
Misc Feature(2)427..603(+)
Misc Feature(3)2626..2793(+)
Misc Feature(4)3628..3795(+)
Misc Feature(5)4306..4452(+)
Misc Feature(6)4471..4800(+)
Misc Feature(7)4801..>4893(+)
Translation

Target ORF information:

RefSeq Version XM_005246276
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005246276

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGTATTCA TGGTCTCCAG GGAGATAAGG GAGAGCCAGG TTATTCAGAA
GGTACAAGGC CAGGACCACC GGGACCAACG GGGGATCCAG GACTGCCGGG TGATATGGGA
AAGAAAGGAG AAATGGGGCA ACCTGGCCCA CCTGGACATT TGGGGCCTGC TGGACCTGAG
GGAGCCCCTG GAAGTCCTGG AAGTCCTGGC CTCCCAGGAA AGCCAGGTCC TCATGGTGAT
TTGGGTTTTA AAGGAATCAA AGGCCTCCTG GGCCCTCCAG GAATCAGAGG CCCTCCAGGT
CTTCCAGGAT TTCCAGGATC TCCTGGACCA ATGGGTATAA GAGGTGACCA AGGACGTGAT
GGAATTCCTG GTCCAGCCGG AGAAAAGGGA GAAACGGGTT TATTGAGGGC CCCTCCAGGC
CCAAGAGGGA ACCCTGGTGC TCAAGGAGCC AAAGGAGACA GGGGAGCCCC AGGTTTTCCT
GGCCTCCCGG GCAGAAAAGG GGCCATGGGA GATGCTGGAC CTCGAGGACC CACAGGCATA
GAAGGATTCC CAGGGCCACC AGGTCTGCCC GGTGCAATTA TCCCTGGCCA GACAGGAAAT
CGTGGTCCAC CAGGCTCAAG AGGAAGCCCA GGTGCGCCTG GTCCCCCTGG ACCTCCAGGG
AGTCATGTAA TAGGCATAAA AGGAGACAAA GGGTCTATGG GCCACCCTGG CCCAAAAGGT
CCACCTGGAA CTGCAGGAGA CATGGGACCA CCAGGTCGTC TGGGAGCACC AGGTACTCCA
GGTCTTCCAG GACCCAGAGG TGATCCTGGA TTCCAGGGGT TTCCAGGCGT GAAAGGAGAA
AAGGGTAATC CTGGATTTCT AGGATCCATT GGACCTCCAG GACCAATTGG GCCAAAAGGA
CCACCTGGTG TACGTGGAGA CCCTGGCACA CTTAAGATTA TCTCCCTTCC AGGAAGCCCA
GGGCCACCTG GCACACCTGG AGAACCAGGG ATGCAGGGAG AACCTGGGCC ACCAGGGCCA
CCTGGAAACC TAGGACCCTG TGGGCCAAGA GGTAAGCCAG GCAAGGATGG AAAACCAGGA
ACTCCTGGAC CAGCTGGAGA AAAAGGCAAC AAAGGTTCTA AAGGAGAGCC AGGACCAGCT
GGATCAGATG GATTGCCAGG TTTGAAAGGA AAACGTGGAG ACAGTGGATC ACCTGCAACC
TGGACAACGA GAGGCTTTGT CTTCACCCGA CACAGTCAAA CCACAGCAAT TCCTTCATGT
CCAGAGGGGA CAGTGCCACT CTACAGTGGG TTTTCTTTTC TTTTTGTACA AGGAAATCAA
CGAGCCCACG GACAAGACCT TGGAACTCTT GGCAGCTGCC TGCAGCGATT TACCACAATG
CCATTCTTAT TCTGCAATGT CAATGATGTA TGTAATTTTG CATCTCGAAA TGATTATTCA
TACTGGCTGT CAACACCAGC TCTGATGCCA ATGAACATGG CTCCCATTAC TGGCAGAGCC
CTTGAGCCTT ATATAAGCAG ATGCACTGTT TGTGAAGGTC CTGCGATCGC CATAGCCGTT
CACAGCCAAA CCACTGACAT TCCTCCATGT CCTCACGGCT GGATTTCTCT CTGGAAAGGA
TTTTCATTCA TCATGAAAGC CTATTCCATC AACTGTGAAA GCTGGGGAAT TAGAAAAAAT
AATAAGTCGC TGTCAGGTGT GCATGAAGAA AAGACACTGA AGCTAAAAAA GACAGCAGAA
CTGCTATTTT TCATCCTAAA GAACAAAGTA ATGACAGAAC ATGCTGTTAT TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35828
Clone ID Related Accession (Same CDS sequence) XM_005246277
Accession Version XM_005246277.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4908bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-3(IV) chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530369764. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GAGACCAAGG TTTTCCAGGT
ACAAAAGGAT CACTGGGTTG TCCTGGAAAA ATGGGAGAGC CTGGGTTACC TGGAAAGCCA
GGCCTCCCAG GAGCCAAGGG AGAACCAGCA GTAGCCATGC CTGGAGGACC AGGAACACCA
GGTTTTCCAG GAGAAAGAGG CAATTCTGGG GAACATGGAG AAATTGGACT CCCTGGACTT
CCAGGTCTCC CTGGAACTCC AGGAAATGAA GGGCTTGATG GACCACGAGG AGATCCAGGG
CAGCCTGGAC CACCTGGAGA ACAAGGACCC CCAGGAAGGT GCATAGAGGG TCCCAGGGGA
GCCCAAGGAC TTCCAGGCTT AAATGGATTG AAAGGGCAAC AAGGCAGAAG AGGTAAAACG
GGGCCAAAGG GAGACCCAGG AATTCCAGGC TTGGATAGAT CAGGATTTCC TGGAGAAACT
GGATCACCAG GAATTCCAGG TCATCAAGGT GAAATGGGAC CACTGGGTCA AAGAGGATAT
CCAGGAAATC CGGGAATTTT AGGGCCACCA GGTGAAGATG GAGTGATTGG GATGATGGGC
TTTCCTGGAG CCATTGGCCC TCCAGGGCCC CCTGGGAACC CAGGCACACC AGGGCAGAGG
GGGAGCCCTG GAATTCCAGG AGTAAAGGGC CAGAGAGGAA CCCCAGGAGC CAAGGGGGAA
CAAGGAGATA AAGGAAATCC CGGGCCTTCA GAGATATCCC ACGTAATAGG GGACAAAGGA
GAACCAGGTC TCAAAGGATT CGCAGGAAAT CCAGGTGAGA AAGGAAACAG AGGCGTTCCA
GGGATGCCAG GTTTAAAGGG CCTCAAAGGA CTACCCGGAC CAGCAGGACC ACCAGGCCCC
AGAGGAGATT TGGGCAGCAC TGGGAATCCT GGAGAACCAG GACTGCGTGG TATACCAGGA
AGCATGGGGA ACATGGGCAT GCCAGGTTCT AAAGGAAAAA GGGGAACTTT GGGATTCCCA
GGTCGAGCAG GAAGACCAGG CCTCCCAGGT ATTCATGGTC TCCAGGGAGA TAAGGGAGAG
CCAGGTTATT CAGAAGGTAC AAGGCCAGGA CCACCGGGAC CAACGGGGGA TCCAGGACTG
CCGGGTGATA TGGGAAAGAA AGGAGAAATG GGGCAACCTG GCCCACCTGG ACATTTGGGG
CCTGCTGGAC CTGAGGGAGC CCCTGGAAGT CCTGGAAGTC CTGGCCTCCC AGGAAAGCCA
GGTCCTCATG GTGATTTGGG TTTTAAAGGA ATCAAAGGCC TCCTGGGCCC TCCAGGAATC
AGAGGCCCTC CAGGTCTTCC AGGATTTCCA GGATCTCCTG GACCAATGGG TATAAGAGGT
GACCAAGGAC GTGATGGAAT TCCTGGTCCA GCCGGAGAAA AGGGAGAAAC GGGTTTATTG
AGGGCCCCTC CAGGCCCAAG AGGGAACCCT GGTGCTCAAG GAGCCAAAGG AGACAGGGGA
GCCCCAGGTT TTCCTGGCCT CCCGGGCAGA AAAGGGGCCA TGGGAGATGC TGGACCTCGA
GGACCCACAG GCATAGAAGG ATTCCCAGGG CCACCAGGTC TGCCCGGTGC AATTATCCCT
GGCCAGACAG GAAATCGTGG TCCACCAGGC TCAAGAGGAA GCCCAGGTGC GCCTGGTCCC
CCTGGACCTC CAGGGAGTCA TGTAATAGGC ATAAAAGGAG ACAAAGGGTC TATGGGCCAC
CCTGGCCCAA AAGGTCCACC TGGAACTGCA GGAGACATGG GACCACCAGG TCGTCTGGGA
GCACCAGGTA CTCCAGGTCT TCCAGGACCC AGAGGTGATC CTGGATTCCA GGGGTTTCCA
GGCGTGAAAG GAGAAAAGGG TAATCCTGGA TTTCTAGGAT CCATTGGACC TCCAGGACCA
ATTGGGCCAA AAGGACCACC TGGTGTACGT GGAGACCCTG GCACACTTAA GATTATCTCC
CTTCCAGGAA GCCCAGGGCC ACCTGGCACA CCTGGAGAAC CAGGGATGCA GGGAGAACCT
GGGCCACCAG GGCCACCTGG AAACCTAGGA CCCTGTGGGC CAAGAGGTAA GCCAGGCAAG
GATGGAAAAC CAGGAACTCC TGGACCAGCT GGAGAAAAAG GCAACAAAGG TTCTAAAGGA
GAGCCAGGAC CAGCTGGATC AGATGGATTG CCAGGTTTGA AAGGAAAACG TGGAGACAGT
GGATCACCTG CAACCTGGAC AACGAGAGGC TTTGTCTTCA CCCGACACAG TCAAACCACA
GCAATTCCTT CATGTCCAGA GGGGACAGTG CCACTCTACA GTGGGTTTTC TTTTCTTTTT
GTACAAGGAA ATCAACGAGC CCACGGACAA GACCTTGGAA CTCTTGGCAG CTGCCTGCAG
CGATTTACCA CAATGCCATT CTTATTCTGC AATGTCAATG ATGTATGTAA TTTTGCATCT
CGAAATGATT ATTCATACTG GCTGTCAACA CCAGCTCTGA TGCCAATGAA CATGGCTCCC
ATTACTGGCA GAGCCCTTGA GCCTTATATA AGCAGATGCA CTGTTTGTGA AGGTCCTGCG
ATCGCCATAG CCGTTCACAG CCAAACCACT GACATTCCTC CATGTCCTCA CGGCTGGATT
TCTCTCTGGA AAGGATTTTC ATTCATCATG TTCACAAGTG CAGGTTCTGA GGGCACCGGG
CAAGCACTGG CCTCCCCTGG CTCCTGCCTG GAAGAATTCC GAGCCAGCCC ATTTCTAGAA
TGTCATGGAA GAGGAACGTG CAACTACTAT TCAAATTCCT ACAGTTTCTG GCTGGCTTCA
TTAAACCCAG AAAGAATGTT CAGAAAGCCT ATTCCATCAA CTGTGAAAGC TGGGGAATTA
GAAAAAATAA TAAGTCGCTG TCAGGTGTGC ATGAAGAAAA GACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005246334.1
CDS139..5046
Misc Feature(1)337..510(+)
Misc Feature(2)427..603(+)
Misc Feature(3)2521..2688(+)
Misc Feature(4)3523..3690(+)
Misc Feature(5)4201..4347(+)
Misc Feature(6)4366..4695(+)
Misc Feature(7)4696..5034(+)
Translation

Target ORF information:

RefSeq Version XM_005246277
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005246277

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GAGACCAAGG TTTTCCAGGT
ACAAAAGGAT CACTGGGTTG TCCTGGAAAA ATGGGAGAGC CTGGGTTACC TGGAAAGCCA
GGCCTCCCAG GAGCCAAGGG AGAACCAGCA GTAGCCATGC CTGGAGGACC AGGAACACCA
GGTTTTCCAG GAGAAAGAGG CAATTCTGGG GAACATGGAG AAATTGGACT CCCTGGACTT
CCAGGTCTCC CTGGAACTCC AGGAAATGAA GGGCTTGATG GACCACGAGG AGATCCAGGG
CAGCCTGGAC CACCTGGAGA ACAAGGACCC CCAGGAAGGT GCATAGAGGG TCCCAGGGGA
GCCCAAGGAC TTCCAGGCTT AAATGGATTG AAAGGGCAAC AAGGCAGAAG AGGTAAAACG
GGGCCAAAGG GAGACCCAGG AATTCCAGGC TTGGATAGAT CAGGATTTCC TGGAGAAACT
GGATCACCAG GAATTCCAGG TCATCAAGGT GAAATGGGAC CACTGGGTCA AAGAGGATAT
CCAGGAAATC CGGGAATTTT AGGGCCACCA GGTGAAGATG GAGTGATTGG GATGATGGGC
TTTCCTGGAG CCATTGGCCC TCCAGGGCCC CCTGGGAACC CAGGCACACC AGGGCAGAGG
GGGAGCCCTG GAATTCCAGG AGTAAAGGGC CAGAGAGGAA CCCCAGGAGC CAAGGGGGAA
CAAGGAGATA AAGGAAATCC CGGGCCTTCA GAGATATCCC ACGTAATAGG GGACAAAGGA
GAACCAGGTC TCAAAGGATT CGCAGGAAAT CCAGGTGAGA AAGGAAACAG AGGCGTTCCA
GGGATGCCAG GTTTAAAGGG CCTCAAAGGA CTACCCGGAC CAGCAGGACC ACCAGGCCCC
AGAGGAGATT TGGGCAGCAC TGGGAATCCT GGAGAACCAG GACTGCGTGG TATACCAGGA
AGCATGGGGA ACATGGGCAT GCCAGGTTCT AAAGGAAAAA GGGGAACTTT GGGATTCCCA
GGTCGAGCAG GAAGACCAGG CCTCCCAGGT ATTCATGGTC TCCAGGGAGA TAAGGGAGAG
CCAGGTTATT CAGAAGGTAC AAGGCCAGGA CCACCGGGAC CAACGGGGGA TCCAGGACTG
CCGGGTGATA TGGGAAAGAA AGGAGAAATG GGGCAACCTG GCCCACCTGG ACATTTGGGG
CCTGCTGGAC CTGAGGGAGC CCCTGGAAGT CCTGGAAGTC CTGGCCTCCC AGGAAAGCCA
GGTCCTCATG GTGATTTGGG TTTTAAAGGA ATCAAAGGCC TCCTGGGCCC TCCAGGAATC
AGAGGCCCTC CAGGTCTTCC AGGATTTCCA GGATCTCCTG GACCAATGGG TATAAGAGGT
GACCAAGGAC GTGATGGAAT TCCTGGTCCA GCCGGAGAAA AGGGAGAAAC GGGTTTATTG
AGGGCCCCTC CAGGCCCAAG AGGGAACCCT GGTGCTCAAG GAGCCAAAGG AGACAGGGGA
GCCCCAGGTT TTCCTGGCCT CCCGGGCAGA AAAGGGGCCA TGGGAGATGC TGGACCTCGA
GGACCCACAG GCATAGAAGG ATTCCCAGGG CCACCAGGTC TGCCCGGTGC AATTATCCCT
GGCCAGACAG GAAATCGTGG TCCACCAGGC TCAAGAGGAA GCCCAGGTGC GCCTGGTCCC
CCTGGACCTC CAGGGAGTCA TGTAATAGGC ATAAAAGGAG ACAAAGGGTC TATGGGCCAC
CCTGGCCCAA AAGGTCCACC TGGAACTGCA GGAGACATGG GACCACCAGG TCGTCTGGGA
GCACCAGGTA CTCCAGGTCT TCCAGGACCC AGAGGTGATC CTGGATTCCA GGGGTTTCCA
GGCGTGAAAG GAGAAAAGGG TAATCCTGGA TTTCTAGGAT CCATTGGACC TCCAGGACCA
ATTGGGCCAA AAGGACCACC TGGTGTACGT GGAGACCCTG GCACACTTAA GATTATCTCC
CTTCCAGGAA GCCCAGGGCC ACCTGGCACA CCTGGAGAAC CAGGGATGCA GGGAGAACCT
GGGCCACCAG GGCCACCTGG AAACCTAGGA CCCTGTGGGC CAAGAGGTAA GCCAGGCAAG
GATGGAAAAC CAGGAACTCC TGGACCAGCT GGAGAAAAAG GCAACAAAGG TTCTAAAGGA
GAGCCAGGAC CAGCTGGATC AGATGGATTG CCAGGTTTGA AAGGAAAACG TGGAGACAGT
GGATCACCTG CAACCTGGAC AACGAGAGGC TTTGTCTTCA CCCGACACAG TCAAACCACA
GCAATTCCTT CATGTCCAGA GGGGACAGTG CCACTCTACA GTGGGTTTTC TTTTCTTTTT
GTACAAGGAA ATCAACGAGC CCACGGACAA GACCTTGGAA CTCTTGGCAG CTGCCTGCAG
CGATTTACCA CAATGCCATT CTTATTCTGC AATGTCAATG ATGTATGTAA TTTTGCATCT
CGAAATGATT ATTCATACTG GCTGTCAACA CCAGCTCTGA TGCCAATGAA CATGGCTCCC
ATTACTGGCA GAGCCCTTGA GCCTTATATA AGCAGATGCA CTGTTTGTGA AGGTCCTGCG
ATCGCCATAG CCGTTCACAG CCAAACCACT GACATTCCTC CATGTCCTCA CGGCTGGATT
TCTCTCTGGA AAGGATTTTC ATTCATCATG TTCACAAGTG CAGGTTCTGA GGGCACCGGG
CAAGCACTGG CCTCCCCTGG CTCCTGCCTG GAAGAATTCC GAGCCAGCCC ATTTCTAGAA
TGTCATGGAA GAGGAACGTG CAACTACTAT TCAAATTCCT ACAGTTTCTG GCTGGCTTCA
TTAAACCCAG AAAGAATGTT CAGAAAGCCT ATTCCATCAA CTGTGAAAGC TGGGGAATTA
GAAAAAATAA TAAGTCGCTG TCAGGTGTGC ATGAAGAAAA GACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56969
Clone ID Related Accession (Same CDS sequence) XM_011510555
Accession Version XM_011510555.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-3(IV) chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGTATTCA TGGTCTCCAG GGAGATAAGG GAGAGCCAGG TTATTCAGAA
GGTACAAGGC CAGGACCACC GGGACCAACG GGGGATCCAG GACTGCCGGG TGATATGGGA
AAGAAAGGAG AAATGGGGCA ACCTGGCCCA CCTGGACATT TGGGGCCTGC TGGACCTGAG
GGAGCCCCTG GAAGTCCTGG AAGTCCTGGC CTCCCAGGAA AGCCAGGTCC TCATGGTGAT
TTGGGTTTTA AAGGAATCAA AGGCCTCCTG GGCCCTCCAG GAATCAGAGG CCCTCCAGGT
CTTCCAGGAT TTCCAGGATC TCCTGGACCA ATGGGTATAA GAGGTGACCA AGGACGTGAT
GGAATTCCTG GTCCAGCCGG AGAAAAGGGA GAAACGGGTT TATTGAGGGC CCCTCCAGGC
CCAAGAGGGA ACCCTGGTGC TCAAGGAGCC AAAGGAGACA GGGGAGCCCC AGGTTTTCCT
GGCCTCCCGG GCAGAAAAGG GGCCATGGGA GATGCTGGAC CTCGAGGACC CACAGGCATA
GAAGGATTCC CAGGGCCACC AGGTCTGCCC GGTGCAATTA TCCCTGGCCA GACAGGAAAT
CGTGGTCCAC CAGGCTCAAG AGGAAGCCCA GGTGCGCCTG GTCCCCCTGG ACCTCCAGGG
AGTCATGTAA TAGGCATAAA AGGAGACAAA GGGTCTATGG GCCACCCTGG CCCAAAAGGT
CCACCTGGAA CTGCAGGAGA CATGGGACCA CCAGGTCGTC TGGGAGCACC AGGTACTCCA
GGTCTTCCAG GACCCAGAGG TGATCCTGGA TTCCAGGGGT TTCCAGGCGT GAAAGGAGAA
AAGGGTAATC CTGGATTTCT AGGATCCATT GGACCTCCAG GACCAATTGG GCCAAAAGGA
CCACCTGGTG TACGTGGAGA CCCTGGCACA CTTAAGATTA TCTCCCTTCC AGGAAGCCCA
GGGCCACCTG GCACACCTGG AGAACCAGGG ATGCAGGGAG AACCTGGGCC ACCAGGGCCA
CCTGGAAACC TAGGACCCTG TGGGCCAAGA GGTAAGCCAG GCAAGGATGG AAAACCAGGA
ACTCCTGGAC CAGCTGGAGA AAAAGGCAAC AAAGGTTCTA AAGGAGAGCC AGGACCAGCT
GGATCAGATG GATTGCCAGG TTTGAAAGGA AAACGTGGAG ACAGTGGATC ACCTGCAACC
TGGACAACGA GAGGCTTTGT CTTCACCCGA CACAGTCAAA CCACAGCAAT TCCTTCATGT
CCAGAGGGGA CAGTGCCACT CTACAGTGGG TTTTCTTTTC TTTTTGTACA AGGAAATCAA
CGAGCCCACG GACAAGACCT TGGAACTCTT GGCAGCTGCC TGCAGCGATT TACCACAATG
CCATTCTTAT TCTGCAATGT CAATGATGTA TGTAATTTTG CATCTCGAAA TGATTATTCA
TACTGGCTGT CAACACCAGC TCTGATGCCA ATGAACATGG CTCCCATTAC TGGCAGAGCC
CTTGAGCCTT ATATAAGCAG ATGCACTGTT TGTGAAGGTC CTGCGATCGC CATAGCCGTT
CACAGCCAAA CCACTGACAT TCCTCCATGT CCTCACGGCT GGATTTCTCT CTGGAAAGGA
TTTTCATTCA TCATGGTTCT GAGGGCACCG GGCAAGCACT GGCCTCCCCT GGCTCCTGCC
TGGAAGAATT CCGAGCCAGC CCATTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508857.1
CDS139..4968
Misc Feature(1)337..510(+)
Misc Feature(2)427..603(+)
Misc Feature(3)2626..2793(+)
Misc Feature(4)3628..3795(+)
Misc Feature(5)4306..4452(+)
Misc Feature(6)4471..4800(+)
Misc Feature(7)4801..>4896(+)
Translation

Target ORF information:

RefSeq Version XM_011510555
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011510555

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGTATTCA TGGTCTCCAG GGAGATAAGG GAGAGCCAGG TTATTCAGAA
GGTACAAGGC CAGGACCACC GGGACCAACG GGGGATCCAG GACTGCCGGG TGATATGGGA
AAGAAAGGAG AAATGGGGCA ACCTGGCCCA CCTGGACATT TGGGGCCTGC TGGACCTGAG
GGAGCCCCTG GAAGTCCTGG AAGTCCTGGC CTCCCAGGAA AGCCAGGTCC TCATGGTGAT
TTGGGTTTTA AAGGAATCAA AGGCCTCCTG GGCCCTCCAG GAATCAGAGG CCCTCCAGGT
CTTCCAGGAT TTCCAGGATC TCCTGGACCA ATGGGTATAA GAGGTGACCA AGGACGTGAT
GGAATTCCTG GTCCAGCCGG AGAAAAGGGA GAAACGGGTT TATTGAGGGC CCCTCCAGGC
CCAAGAGGGA ACCCTGGTGC TCAAGGAGCC AAAGGAGACA GGGGAGCCCC AGGTTTTCCT
GGCCTCCCGG GCAGAAAAGG GGCCATGGGA GATGCTGGAC CTCGAGGACC CACAGGCATA
GAAGGATTCC CAGGGCCACC AGGTCTGCCC GGTGCAATTA TCCCTGGCCA GACAGGAAAT
CGTGGTCCAC CAGGCTCAAG AGGAAGCCCA GGTGCGCCTG GTCCCCCTGG ACCTCCAGGG
AGTCATGTAA TAGGCATAAA AGGAGACAAA GGGTCTATGG GCCACCCTGG CCCAAAAGGT
CCACCTGGAA CTGCAGGAGA CATGGGACCA CCAGGTCGTC TGGGAGCACC AGGTACTCCA
GGTCTTCCAG GACCCAGAGG TGATCCTGGA TTCCAGGGGT TTCCAGGCGT GAAAGGAGAA
AAGGGTAATC CTGGATTTCT AGGATCCATT GGACCTCCAG GACCAATTGG GCCAAAAGGA
CCACCTGGTG TACGTGGAGA CCCTGGCACA CTTAAGATTA TCTCCCTTCC AGGAAGCCCA
GGGCCACCTG GCACACCTGG AGAACCAGGG ATGCAGGGAG AACCTGGGCC ACCAGGGCCA
CCTGGAAACC TAGGACCCTG TGGGCCAAGA GGTAAGCCAG GCAAGGATGG AAAACCAGGA
ACTCCTGGAC CAGCTGGAGA AAAAGGCAAC AAAGGTTCTA AAGGAGAGCC AGGACCAGCT
GGATCAGATG GATTGCCAGG TTTGAAAGGA AAACGTGGAG ACAGTGGATC ACCTGCAACC
TGGACAACGA GAGGCTTTGT CTTCACCCGA CACAGTCAAA CCACAGCAAT TCCTTCATGT
CCAGAGGGGA CAGTGCCACT CTACAGTGGG TTTTCTTTTC TTTTTGTACA AGGAAATCAA
CGAGCCCACG GACAAGACCT TGGAACTCTT GGCAGCTGCC TGCAGCGATT TACCACAATG
CCATTCTTAT TCTGCAATGT CAATGATGTA TGTAATTTTG CATCTCGAAA TGATTATTCA
TACTGGCTGT CAACACCAGC TCTGATGCCA ATGAACATGG CTCCCATTAC TGGCAGAGCC
CTTGAGCCTT ATATAAGCAG ATGCACTGTT TGTGAAGGTC CTGCGATCGC CATAGCCGTT
CACAGCCAAA CCACTGACAT TCCTCCATGT CCTCACGGCT GGATTTCTCT CTGGAAAGGA
TTTTCATTCA TCATGGTTCT GAGGGCACCG GGCAAGCACT GGCCTCCCCT GGCTCCTGCC
TGGAAGAATT CCGAGCCAGC CCATTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56970
Clone ID Related Accession (Same CDS sequence) XM_011510556
Accession Version XM_011510556.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-3(IV) chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGGGACTC CTGGGTCCCC AGGTTGTGCT GGTTCACCAG GTCTTCCAGG ATCACCGGGA 
CCTCCAGGAC CGCCAGGTGA CATCGTTTTT CGCAAGGGTC CACCTGGAGA TCACGGACTG
CCAGGCTATC TAGGGTCTCC AGGAATCCCA GGAGTTGATG GGCCCAAAGG AGAACCAGGC
CTCCTGTGTA CACAGTGCCC TTATATCCCA GGGCCTCCCG GTCTCCCAGG ATTGCCAGGG
TTACATGGTG TAAAAGGAAT CCCAGGAAGA CAAGGCGCAG CTGGCTTGAA AGGAAGCCCA
GGGTCCCCAG GAAATACAGG TCTTCCAGGA TTTCCAGGTT TCCCAGGTGC CCAGGGTGAC
CCAGGACTTA AAGGAGAAAA AGGTGAAACA CTTCAGCCTG AGGGGCAAGT GGGTGTCCCA
GGTGACCCGG GGCTCAGAGG CCAACCTGGG AGAAAGGGCT TGGATGGAAT TCCTGGAACT
CCGGGAGTGA AAGGATTACC AGGACCTAAA GGCGAACTGG CTCTGAGTGG TGAGAAAGGG
GACCAAGGTC CTCCAGGGGA TCCTGGCTCC CCTGGGTCCC CAGGACCTGC AGGACCAGCT
GGACCACCTG GCTACGGACC CCAAGGAGAA CCTGGTCTCC AGGGCACGCA AGGAGTTCCT
GGAGCCCCCG GACCACCCGG AGAAGCCGGC CCTAGGGGAG AGCTCAGTGT TTCAACACCA
GTTCCAGGCC CACCAGGACC TCCAGGGCCC CCTGGCCATC CTGGCCCCCA AGGTCCACCT
GGTATCCCTG GATCCCTGGG GAAATGTGGA GATCCTGGTC TTCCAGGGCC TGATGGTGAA
CCAGGAATTC CAGGAATTGG ATTTCCTGGG CCTCCTGGAC CTAAGGGAGA CCAAGGTTTT
CCAGGTACAA AAGGATCACT GGGTTGTCCT GGAAAAATGG GAGAGCCTGG GTTACCTGGA
AAGCCAGGCC TCCCAGGAGC CAAGGGAGAA CCAGCAGTAG CCATGCCTGG AGGACCAGGA
ACACCAGGTT TTCCAGGAGA AAGAGGCAAT TCTGGGGAAC ATGGAGAAAT TGGACTCCCT
GGACTTCCAG GTCTCCCTGG AACTCCAGGA AATGAAGGGC TTGATGGACC ACGAGGAGAT
CCAGGGCAGC CTGGACCACC TGGAGAACAA GGACCCCCAG GAAGGTGCAT AGAGGGTCCC
AGGGGAGCCC AAGGACTTCC AGGCTTAAAT GGATTGAAAG GGCAACAAGG CAGAAGAGGT
AAAACGGGGC CAAAGGGAGA CCCAGGAATT CCAGGCTTGG ATAGATCAGG ATTTCCTGGA
GAAACTGGAT CACCAGGAAT TCCAGGTCAT CAAGGTGAAA TGGGACCACT GGGTCAAAGA
GGATATCCAG GAAATCCGGG AATTTTAGGG CCACCAGGTG AAGATGGAGT GATTGGGATG
ATGGGCTTTC CTGGAGCCAT TGGCCCTCCA GGGCCCCCTG GGAACCCAGG CACACCAGGG
CAGAGGGGGA GCCCTGGAAT TCCAGGAGTA AAGGGCCAGA GAGGAACCCC AGGAGCCAAG
GGGGAACAAG GAGATAAAGG AAATCCCGGG CCTTCAGAGA TATCCCACGT AATAGGGGAC
AAAGGAGAAC CAGGTCTCAA AGGATTCGCA GGAAATCCAG GTGAGAAAGG AAACAGAGGC
GTTCCAGGGA TGCCAGGTTT AAAGGGCCTC AAAGGACTAC CCGGACCAGC AGGACCACCA
GGCCCCAGAG GAGATTTGGG CAGCACTGGG AATCCTGGAG AACCAGGACT GCGTGGTATA
CCAGGAAGCA TGGGGAACAT GGGCATGCCA GGTTCTAAAG GAAAAAGGGG AACTTTGGGA
TTCCCAGGTC GAGCAGGAAG ACCAGGCCTC CCAGGTATTC ATGGTCTCCA GGGAGATAAG
GGAGAGCCAG GTTATTCAGA AGGTACAAGG CCAGGACCAC CGGGACCAAC GGGGGATCCA
GGACTGCCGG GTGATATGGG AAAGAAAGGA GAAATGGGGC AACCTGGCCC ACCTGGACAT
TTGGGGCCTG CTGGACCTGA GGGAGCCCCT GGAAGTCCTG GAAGTCCTGG CCTCCCAGGA
AAGCCAGGTC CTCATGGTGA TTTGGGTTTT AAAGGAATCA AAGGCCTCCT GGGCCCTCCA
GGAATCAGAG GCCCTCCAGG TCTTCCAGGA TTTCCAGGAT CTCCTGGACC AATGGGTATA
AGAGGTGACC AAGGACGTGA TGGAATTCCT GGTCCAGCCG GAGAAAAGGG AGAAACGGGT
TTATTGAGGG CCCCTCCAGG CCCAAGAGGG AACCCTGGTG CTCAAGGAGC CAAAGGAGAC
AGGGGAGCCC CAGGTTTTCC TGGCCTCCCG GGCAGAAAAG GGGCCATGGG AGATGCTGGA
CCTCGAGGAC CCACAGGCAT AGAAGGATTC CCAGGGCCAC CAGGTCTGCC CGGTGCAATT
ATCCCTGGCC AGACAGGAAA TCGTGGTCCA CCAGGCTCAA GAGGAAGCCC AGGTGCGCCT
GGTCCCCCTG GACCTCCAGG GAGTCATGTA ATAGGCATAA AAGGAGACAA AGGGTCTATG
GGCCACCCTG GCCCAAAAGG TCCACCTGGA ACTGCAGGAG ACATGGGACC ACCAGGTCGT
CTGGGAGCAC CAGGTACTCC AGGTCTTCCA GGACCCAGAG GTGATCCTGG ATTCCAGGGG
TTTCCAGGCG TGAAAGGAGA AAAGGGTAAT CCTGGATTTC TAGGATCCAT TGGACCTCCA
GGACCAATTG GGCCAAAAGG ACCACCTGGT GTACGTGGAG ACCCTGGCAC ACTTAAGATT
ATCTCCCTTC CAGGAAGCCC AGGGCCACCT GGCACACCTG GAGAACCAGG GATGCAGGGA
GAACCTGGGC CACCAGGGCC ACCTGGAAAC CTAGGACCCT GTGGGCCAAG AGGTAAGCCA
GGCAAGGATG GAAAACCAGG AACTCCTGGA CCAGCTGGAG AAAAAGGCAA CAAAGGTTCT
AAAGGAGAGC CAGGACCAGC TGGATCAGAT GGATTGCCAG GTTTGAAAGG AAAACGTGGA
GACAGTGGAT CACCTGCAAC CTGGACAACG AGAGGCTTTG TCTTCACCCG ACACAGTCAA
ACCACAGCAA TTCCTTCATG TCCAGAGGGG ACAGTGCCAC TCTACAGTGG GTTTTCTTTT
CTTTTTGTAC AAGGAAATCA ACGAGCCCAC GGACAAGACC TTGGAACTCT TGGCAGCTGC
CTGCAGCGAT TTACCACAAT GCCATTCTTA TTCTGCAATG TCAATGATGT ATGTAATTTT
GCATCTCGAA ATGATTATTC ATACTGGCTG TCAACACCAG CTCTGATGCC AATGAACATG
GCTCCCATTA CTGGCAGAGC CCTTGAGCCT TATATAAGCA GATGCACTGT TTGTGAAGGT
CCTGCGATCG CCATAGCCGT TCACAGCCAA ACCACTGACA TTCCTCCATG TCCTCACGGC
TGGATTTCTC TCTGGAAAGG ATTTTCATTC ATCATGTTCA CAAGTGCAGG TTCTGAGGGC
ACCGGGCAAG CACTGGCCTC CCCTGGCTCC TGCCTGGAAG AATTCCGAGC CAGCCCATTT
CTAGAATGTC ATGGAAGAGG AACGTGCAAC TACTATTCAA ATTCCTACAG TTTCTGGCTG
GCTTCATTAA ACCCAGAAAG AATGTTCAGA AAGCCTATTC CATCAACTGT GAAAGCTGGG
GAATTAGAAA AAATAATAAG TCGCTGTCAG GTGTGCATGA AGAAAAGACA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508858.1
CDS434..4207
Misc Feature(1)1682..1849(+)
Misc Feature(2)2684..2851(+)
Misc Feature(3)3362..3508(+)
Misc Feature(4)3527..3856(+)
Misc Feature(5)3857..4195(+)
Translation

Target ORF information:

RefSeq Version XM_011510556
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011510556

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGGGACTC CTGGGTCCCC AGGTTGTGCT GGTTCACCAG GTCTTCCAGG ATCACCGGGA 
CCTCCAGGAC CGCCAGGTGA CATCGTTTTT CGCAAGGGTC CACCTGGAGA TCACGGACTG
CCAGGCTATC TAGGGTCTCC AGGAATCCCA GGAGTTGATG GGCCCAAAGG AGAACCAGGC
CTCCTGTGTA CACAGTGCCC TTATATCCCA GGGCCTCCCG GTCTCCCAGG ATTGCCAGGG
TTACATGGTG TAAAAGGAAT CCCAGGAAGA CAAGGCGCAG CTGGCTTGAA AGGAAGCCCA
GGGTCCCCAG GAAATACAGG TCTTCCAGGA TTTCCAGGTT TCCCAGGTGC CCAGGGTGAC
CCAGGACTTA AAGGAGAAAA AGGTGAAACA CTTCAGCCTG AGGGGCAAGT GGGTGTCCCA
GGTGACCCGG GGCTCAGAGG CCAACCTGGG AGAAAGGGCT TGGATGGAAT TCCTGGAACT
CCGGGAGTGA AAGGATTACC AGGACCTAAA GGCGAACTGG CTCTGAGTGG TGAGAAAGGG
GACCAAGGTC CTCCAGGGGA TCCTGGCTCC CCTGGGTCCC CAGGACCTGC AGGACCAGCT
GGACCACCTG GCTACGGACC CCAAGGAGAA CCTGGTCTCC AGGGCACGCA AGGAGTTCCT
GGAGCCCCCG GACCACCCGG AGAAGCCGGC CCTAGGGGAG AGCTCAGTGT TTCAACACCA
GTTCCAGGCC CACCAGGACC TCCAGGGCCC CCTGGCCATC CTGGCCCCCA AGGTCCACCT
GGTATCCCTG GATCCCTGGG GAAATGTGGA GATCCTGGTC TTCCAGGGCC TGATGGTGAA
CCAGGAATTC CAGGAATTGG ATTTCCTGGG CCTCCTGGAC CTAAGGGAGA CCAAGGTTTT
CCAGGTACAA AAGGATCACT GGGTTGTCCT GGAAAAATGG GAGAGCCTGG GTTACCTGGA
AAGCCAGGCC TCCCAGGAGC CAAGGGAGAA CCAGCAGTAG CCATGCCTGG AGGACCAGGA
ACACCAGGTT TTCCAGGAGA AAGAGGCAAT TCTGGGGAAC ATGGAGAAAT TGGACTCCCT
GGACTTCCAG GTCTCCCTGG AACTCCAGGA AATGAAGGGC TTGATGGACC ACGAGGAGAT
CCAGGGCAGC CTGGACCACC TGGAGAACAA GGACCCCCAG GAAGGTGCAT AGAGGGTCCC
AGGGGAGCCC AAGGACTTCC AGGCTTAAAT GGATTGAAAG GGCAACAAGG CAGAAGAGGT
AAAACGGGGC CAAAGGGAGA CCCAGGAATT CCAGGCTTGG ATAGATCAGG ATTTCCTGGA
GAAACTGGAT CACCAGGAAT TCCAGGTCAT CAAGGTGAAA TGGGACCACT GGGTCAAAGA
GGATATCCAG GAAATCCGGG AATTTTAGGG CCACCAGGTG AAGATGGAGT GATTGGGATG
ATGGGCTTTC CTGGAGCCAT TGGCCCTCCA GGGCCCCCTG GGAACCCAGG CACACCAGGG
CAGAGGGGGA GCCCTGGAAT TCCAGGAGTA AAGGGCCAGA GAGGAACCCC AGGAGCCAAG
GGGGAACAAG GAGATAAAGG AAATCCCGGG CCTTCAGAGA TATCCCACGT AATAGGGGAC
AAAGGAGAAC CAGGTCTCAA AGGATTCGCA GGAAATCCAG GTGAGAAAGG AAACAGAGGC
GTTCCAGGGA TGCCAGGTTT AAAGGGCCTC AAAGGACTAC CCGGACCAGC AGGACCACCA
GGCCCCAGAG GAGATTTGGG CAGCACTGGG AATCCTGGAG AACCAGGACT GCGTGGTATA
CCAGGAAGCA TGGGGAACAT GGGCATGCCA GGTTCTAAAG GAAAAAGGGG AACTTTGGGA
TTCCCAGGTC GAGCAGGAAG ACCAGGCCTC CCAGGTATTC ATGGTCTCCA GGGAGATAAG
GGAGAGCCAG GTTATTCAGA AGGTACAAGG CCAGGACCAC CGGGACCAAC GGGGGATCCA
GGACTGCCGG GTGATATGGG AAAGAAAGGA GAAATGGGGC AACCTGGCCC ACCTGGACAT
TTGGGGCCTG CTGGACCTGA GGGAGCCCCT GGAAGTCCTG GAAGTCCTGG CCTCCCAGGA
AAGCCAGGTC CTCATGGTGA TTTGGGTTTT AAAGGAATCA AAGGCCTCCT GGGCCCTCCA
GGAATCAGAG GCCCTCCAGG TCTTCCAGGA TTTCCAGGAT CTCCTGGACC AATGGGTATA
AGAGGTGACC AAGGACGTGA TGGAATTCCT GGTCCAGCCG GAGAAAAGGG AGAAACGGGT
TTATTGAGGG CCCCTCCAGG CCCAAGAGGG AACCCTGGTG CTCAAGGAGC CAAAGGAGAC
AGGGGAGCCC CAGGTTTTCC TGGCCTCCCG GGCAGAAAAG GGGCCATGGG AGATGCTGGA
CCTCGAGGAC CCACAGGCAT AGAAGGATTC CCAGGGCCAC CAGGTCTGCC CGGTGCAATT
ATCCCTGGCC AGACAGGAAA TCGTGGTCCA CCAGGCTCAA GAGGAAGCCC AGGTGCGCCT
GGTCCCCCTG GACCTCCAGG GAGTCATGTA ATAGGCATAA AAGGAGACAA AGGGTCTATG
GGCCACCCTG GCCCAAAAGG TCCACCTGGA ACTGCAGGAG ACATGGGACC ACCAGGTCGT
CTGGGAGCAC CAGGTACTCC AGGTCTTCCA GGACCCAGAG GTGATCCTGG ATTCCAGGGG
TTTCCAGGCG TGAAAGGAGA AAAGGGTAAT CCTGGATTTC TAGGATCCAT TGGACCTCCA
GGACCAATTG GGCCAAAAGG ACCACCTGGT GTACGTGGAG ACCCTGGCAC ACTTAAGATT
ATCTCCCTTC CAGGAAGCCC AGGGCCACCT GGCACACCTG GAGAACCAGG GATGCAGGGA
GAACCTGGGC CACCAGGGCC ACCTGGAAAC CTAGGACCCT GTGGGCCAAG AGGTAAGCCA
GGCAAGGATG GAAAACCAGG AACTCCTGGA CCAGCTGGAG AAAAAGGCAA CAAAGGTTCT
AAAGGAGAGC CAGGACCAGC TGGATCAGAT GGATTGCCAG GTTTGAAAGG AAAACGTGGA
GACAGTGGAT CACCTGCAAC CTGGACAACG AGAGGCTTTG TCTTCACCCG ACACAGTCAA
ACCACAGCAA TTCCTTCATG TCCAGAGGGG ACAGTGCCAC TCTACAGTGG GTTTTCTTTT
CTTTTTGTAC AAGGAAATCA ACGAGCCCAC GGACAAGACC TTGGAACTCT TGGCAGCTGC
CTGCAGCGAT TTACCACAAT GCCATTCTTA TTCTGCAATG TCAATGATGT ATGTAATTTT
GCATCTCGAA ATGATTATTC ATACTGGCTG TCAACACCAG CTCTGATGCC AATGAACATG
GCTCCCATTA CTGGCAGAGC CCTTGAGCCT TATATAAGCA GATGCACTGT TTGTGAAGGT
CCTGCGATCG CCATAGCCGT TCACAGCCAA ACCACTGACA TTCCTCCATG TCCTCACGGC
TGGATTTCTC TCTGGAAAGG ATTTTCATTC ATCATGTTCA CAAGTGCAGG TTCTGAGGGC
ACCGGGCAAG CACTGGCCTC CCCTGGCTCC TGCCTGGAAG AATTCCGAGC CAGCCCATTT
CTAGAATGTC ATGGAAGAGG AACGTGCAAC TACTATTCAA ATTCCTACAG TTTCTGGCTG
GCTTCATTAA ACCCAGAAAG AATGTTCAGA AAGCCTATTC CATCAACTGT GAAAGCTGGG
GAATTAGAAA AAATAATAAG TCGCTGTCAG GTGTGCATGA AGAAAAGACA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35830
Clone ID Related Accession (Same CDS sequence) XM_006712245
Accession Version XM_006712245.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-3(IV) chain isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578803610. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGTATTCA TGGTCTCCAG GGAGATAAGG GAGAGCCAGG TTATTCAGAA
GGTACAAGGC CAGGACCACC GGGACCAACG GGGGATCCAG GACTGCCGGG TGATATGGGA
AAGAAAGGAG AAATGGGGCA ACCTGGCCCA CCTGGACATT TGGGGCCTGC TGGACCTGAG
GGAGCCCCTG GAAGTCCTGG AAGTCCTGGC CTCCCAGGAA AGCCAGGTCC TCATGGTGAT
TTGGGTTTTA AAGGAATCAA AGGCCTCCTG GGCCCTCCAG GAATCAGAGG CCCTCCAGGT
CTTCCAGGAT TTCCAGGATC TCCTGGACCA ATGGGTATAA GAGGTGACCA AGGACGTGAT
GGAATTCCTG GTCCAGCCGG AGAAAAGGGA GAAACGGGTT TATTGAGGGC CCCTCCAGGC
CCAAGAGGGA ACCCTGGTGC TCAAGGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006712308.1
CDS139..3708
Misc Feature(1)337..510(+)
Misc Feature(2)427..603(+)
Misc Feature(3)2626..2793(+)
Translation

Target ORF information:

RefSeq Version XM_006712245
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006712245

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGTATTCA TGGTCTCCAG GGAGATAAGG GAGAGCCAGG TTATTCAGAA
GGTACAAGGC CAGGACCACC GGGACCAACG GGGGATCCAG GACTGCCGGG TGATATGGGA
AAGAAAGGAG AAATGGGGCA ACCTGGCCCA CCTGGACATT TGGGGCCTGC TGGACCTGAG
GGAGCCCCTG GAAGTCCTGG AAGTCCTGGC CTCCCAGGAA AGCCAGGTCC TCATGGTGAT
TTGGGTTTTA AAGGAATCAA AGGCCTCCTG GGCCCTCCAG GAATCAGAGG CCCTCCAGGT
CTTCCAGGAT TTCCAGGATC TCCTGGACCA ATGGGTATAA GAGGTGACCA AGGACGTGAT
GGAATTCCTG GTCCAGCCGG AGAAAAGGGA GAAACGGGTT TATTGAGGGC CCCTCCAGGC
CCAAGAGGGA ACCCTGGTGC TCAAGGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35832
Clone ID Related Accession (Same CDS sequence) XM_005246280
Accession Version XM_005246280.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3156bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-3(IV) chain isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005403.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530369771. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGGGGATC CAGGACTGCC GGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005246337.1
CDS139..3294
Misc Feature(1)337..510(+)
Misc Feature(2)427..603(+)
Misc Feature(3)2626..2793(+)
Translation

Target ORF information:

RefSeq Version XM_005246280
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005246280

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGGGGATC CAGGACTGCC GGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23179
Clone ID Related Accession (Same CDS sequence) NM_000091
Accession Version NM_000091.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5013bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-3(IV) chain precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X80031.1, AU099354.1, AC107069.5, CD675393.1, AC097662.5, BM712932.1 and BM670570.1. This sequence is a reference standard in the RefSeqGene project. On Jun 8, 2010 this sequence version replaced gi:89142729. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X80031.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGTATTCA TGGTCTCCAG GGAGATAAGG GAGAGCCAGG TTATTCAGAA
GGTACAAGGC CAGGACCACC GGGACCAACG GGGGATCCAG GACTGCCGGG TGATATGGGA
AAGAAAGGAG AAATGGGGCA ACCTGGCCCA CCTGGACATT TGGGGCCTGC TGGACCTGAG
GGAGCCCCTG GAAGTCCTGG AAGTCCTGGC CTCCCAGGAA AGCCAGGTCC TCATGGTGAT
TTGGGTTTTA AAGGAATCAA AGGCCTCCTG GGCCCTCCAG GAATCAGAGG CCCTCCAGGT
CTTCCAGGAT TTCCAGGATC TCCTGGACCA ATGGGTATAA GAGGTGACCA AGGACGTGAT
GGAATTCCTG GTCCAGCCGG AGAAAAGGGA GAAACGGGTT TATTGAGGGC CCCTCCAGGC
CCAAGAGGGA ACCCTGGTGC TCAAGGAGCC AAAGGAGACA GGGGAGCCCC AGGTTTTCCT
GGCCTCCCGG GCAGAAAAGG GGCCATGGGA GATGCTGGAC CTCGAGGACC CACAGGCATA
GAAGGATTCC CAGGGCCACC AGGTCTGCCC GGTGCAATTA TCCCTGGCCA GACAGGAAAT
CGTGGTCCAC CAGGCTCAAG AGGAAGCCCA GGTGCGCCTG GTCCCCCTGG ACCTCCAGGG
AGTCATGTAA TAGGCATAAA AGGAGACAAA GGGTCTATGG GCCACCCTGG CCCAAAAGGT
CCACCTGGAA CTGCAGGAGA CATGGGACCA CCAGGTCGTC TGGGAGCACC AGGTACTCCA
GGTCTTCCAG GACCCAGAGG TGATCCTGGA TTCCAGGGGT TTCCAGGCGT GAAAGGAGAA
AAGGGTAATC CTGGATTTCT AGGATCCATT GGACCTCCAG GACCAATTGG GCCAAAAGGA
CCACCTGGTG TACGTGGAGA CCCTGGCACA CTTAAGATTA TCTCCCTTCC AGGAAGCCCA
GGGCCACCTG GCACACCTGG AGAACCAGGG ATGCAGGGAG AACCTGGGCC ACCAGGGCCA
CCTGGAAACC TAGGACCCTG TGGGCCAAGA GGTAAGCCAG GCAAGGATGG AAAACCAGGA
ACTCCTGGAC CAGCTGGAGA AAAAGGCAAC AAAGGTTCTA AAGGAGAGCC AGGACCAGCT
GGATCAGATG GATTGCCAGG TTTGAAAGGA AAACGTGGAG ACAGTGGATC ACCTGCAACC
TGGACAACGA GAGGCTTTGT CTTCACCCGA CACAGTCAAA CCACAGCAAT TCCTTCATGT
CCAGAGGGGA CAGTGCCACT CTACAGTGGG TTTTCTTTTC TTTTTGTACA AGGAAATCAA
CGAGCCCACG GACAAGACCT TGGAACTCTT GGCAGCTGCC TGCAGCGATT TACCACAATG
CCATTCTTAT TCTGCAATGT CAATGATGTA TGTAATTTTG CATCTCGAAA TGATTATTCA
TACTGGCTGT CAACACCAGC TCTGATGCCA ATGAACATGG CTCCCATTAC TGGCAGAGCC
CTTGAGCCTT ATATAAGCAG ATGCACTGTT TGTGAAGGTC CTGCGATCGC CATAGCCGTT
CACAGCCAAA CCACTGACAT TCCTCCATGT CCTCACGGCT GGATTTCTCT CTGGAAAGGA
TTTTCATTCA TCATGTTCAC AAGTGCAGGT TCTGAGGGCA CCGGGCAAGC ACTGGCCTCC
CCTGGCTCCT GCCTGGAAGA ATTCCGAGCC AGCCCATTTC TAGAATGTCA TGGAAGAGGA
ACGTGCAACT ACTATTCAAA TTCCTACAGT TTCTGGCTGG CTTCATTAAA CCCAGAAAGA
ATGTTCAGAA AGCCTATTCC ATCAACTGTG AAAGCTGGGG AATTAGAAAA AATAATAAGT
CGCTGTCAGG TGTGCATGAA GAAAAGACAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000082.2
CDS163..5175
Misc Feature(1)112..114(+)
Misc Feature(2)247..288(+)
Misc Feature(3)289..4476(+)
Misc Feature(4)361..534(+)
Misc Feature(5)451..627(+)
Misc Feature(6)2533..2541(+)
Misc Feature(7)2650..2817(+)
Misc Feature(8)3148..3156(+)
Misc Feature(9)3622..3630(+)
Misc Feature(10)3652..3819(+)
Misc Feature(11)4078..4086(+)
Misc Feature(12)4195..4203(+)
Misc Feature(13)4330..4476(+)
Misc Feature(14)4438..4443(+)
Misc Feature(15)4441..4494(+)
Misc Feature(16)4456..4464(+)
Misc Feature(17)4495..4824(+)
Misc Feature(18)4597..4833(+)
Misc Feature(19)4825..5163(+)
Misc Feature(20)4990..5046(+)
Exon (1)1..249
Gene:COL4A3
Gene Synonym:
Exon (2)250..306
Gene:COL4A3
Gene Synonym:
Exon (3)307..396
Gene:COL4A3
Gene Synonym:
Exon (4)397..441
Gene:COL4A3
Gene Synonym:
Exon (5)442..486
Gene:COL4A3
Gene Synonym:
Exon (6)487..549
Gene:COL4A3
Gene Synonym:
Exon (7)550..603
Gene:COL4A3
Gene Synonym:
Exon (8)604..630
Gene:COL4A3
Gene Synonym:
Exon (9)631..708
Gene:COL4A3
Gene Synonym:
Exon (10)709..771
Gene:COL4A3
Gene Synonym:
Exon (11)772..807
Gene:COL4A3
Gene Synonym:
Exon (12)808..849
Gene:COL4A3
Gene Synonym:
Exon (13)850..927
Gene:COL4A3
Gene Synonym:
Exon (14)928..990
Gene:COL4A3
Gene Synonym:
Exon (15)991..1050
Gene:COL4A3
Gene Synonym:
Exon (16)1051..1095
Gene:COL4A3
Gene Synonym:
Exon (17)1096..1149
Gene:COL4A3
Gene Synonym:
Exon (18)1150..1191
Gene:COL4A3
Gene Synonym:
Exon (19)1192..1276
Gene:COL4A3
Gene Synonym:
Exon (20)1277..1312
Gene:COL4A3
Gene Synonym:
Exon (21)1313..1477
Gene:COL4A3
Gene Synonym:
Exon (22)1478..1570
Gene:COL4A3
Gene Synonym:
Exon (23)1571..1666
Gene:COL4A3
Gene Synonym:
Exon (24)1667..1737
Gene:COL4A3
Gene Synonym:
Exon (25)1738..1920
Gene:COL4A3
Gene Synonym:
Exon (26)1921..2089
Gene:COL4A3
Gene Synonym:
Exon (27)2090..2182
Gene:COL4A3
Gene Synonym:
Exon (28)2183..2287
Gene:COL4A3
Gene Synonym:
Exon (29)2288..2385
Gene:COL4A3
Gene Synonym:
Exon (30)2386..2536
Gene:COL4A3
Gene Synonym:
Exon (31)2537..2650
Gene:COL4A3
Gene Synonym:
Exon (32)2651..2818
Gene:COL4A3
Gene Synonym:
Exon (33)2819..2908
Gene:COL4A3
Gene Synonym:
Exon (34)2909..3043
Gene:COL4A3
Gene Synonym:
Exon (35)3044..3142
Gene:COL4A3
Gene Synonym:
Exon (36)3143..3232
Gene:COL4A3
Gene Synonym:
Exon (37)3233..3372
Gene:COL4A3
Gene Synonym:
Exon (38)3373..3499
Gene:COL4A3
Gene Synonym:
Exon (39)3500..3580
Gene:COL4A3
Gene Synonym:
Exon (40)3581..3679
Gene:COL4A3
Gene Synonym:
Exon (41)3680..3727
Gene:COL4A3
Gene Synonym:
Exon (42)3728..3913
Gene:COL4A3
Gene Synonym:
Exon (43)3914..4044
Gene:COL4A3
Gene Synonym:
Exon (44)4045..4117
Gene:COL4A3
Gene Synonym:
Exon (45)4118..4189
Gene:COL4A3
Gene Synonym:
Exon (46)4190..4315
Gene:COL4A3
Gene Synonym:
Exon (47)4316..4414
Gene:COL4A3
Gene Synonym:
Exon (48)4415..4624
Gene:COL4A3
Gene Synonym:
Exon (49)4625..4802
Gene:COL4A3
Gene Synonym:
Exon (50)4803..4917
Gene:COL4A3
Gene Synonym:
Exon (51)4918..5090
Gene:COL4A3
Gene Synonym:
Exon (52)5091..8097
Gene:COL4A3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000091
Organism Homo sapiens (human)
Definition Homo sapiens collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000091

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
ATGAGCGCCC GGACCGCCCC CAGGCCGCAG GTGCTCCTGC TGCCGCTCCT GCTGGTGCTC 
CTGGCGGCGG CGCCCGCAGC CAGCAAGGGT TGTGTCTGTA AAGACAAAGG CCAGTGCTTC
TGTGACGGGG CCAAAGGGGA GAAGGGGGAG AAGGGCTTTC CTGGACCCCC CGGTTCTCCT
GGCCAGAAAG GATTCACAGG TCCTGAAGGC TTGCCTGGAC CGCAGGGACC CAAGGGCTTT
CCAGGACTTC CAGGACTCAC GGGTTCCAAA GGTGTAAGGG GAATAAGTGG ATTGCCAGGA
TTTTCTGGTT CTCCTGGACT TCCAGGCACC CCAGGCAATA CCGGGCCTTA CGGACTTGTC
GGTGTACCAG GATGCAGTGG TTCTAAGGGT GAGCAGGGGT TTCCAGGACT CCCAGGGACA
CTGGGCTACC CAGGGATCCC GGGTGCTGCT GGTTTGAAAG GACAAAAGGG TGCTCCTGCT
AAAGAAGAAG ATATAGAACT TGATGCAAAA GGCGACCCCG GGTTGCCAGG GGCTCCAGGA
CCCCAGGGTT TGCCAGGCCC TCCAGGTTTT CCTGGGCCTG TTGGCCCACC TGGTCCTCCG
GGATTCTTTG GCTTTCCAGG AGCCATGGGA CCTAGAGGAC CTAAGGGTCA CATGGGTGAA
AGAGTGATAG GACATAAAGG AGAGCGGGGT GTGAAAGGGT TAACAGGACC CCCGGGACCA
CCAGGAACAG TTATTGTGAC CCTAACTGGC CCAGATAACA GAACGGACCT CAAGGGGGAA
AAGGGAGACA AGGGAGCAAT GGGCGAGCCT GGACCTCCTG GACCCTCAGG ACTGCCTGGA
GAATCATATG GATCTGAAAA GGGTGCTCCT GGAGACCCTG GCCTGCAGGG AAAACCCGGA
AAAGATGGTG TTCCTGGCTT CCCTGGAAGT GAGGGAGTCA AGGGCAACAG GGGTTTCCCT
GGGTTAATGG GTGAAGATGG CATTAAGGGA CAGAAAGGGG ACATTGGCCC TCCAGGATTT
CGTGGTCCAA CAGAATATTA TGACACATAC CAGGAAAAGG GAGATGAAGG CACTCCAGGC
CCACCAGGGC CCAGAGGAGC TCGTGGCCCA CAAGGTCCCA GTGGTCCCCC CGGAGTTCCT
GGAAGTCCTG GATCATCAAG GCCTGGCCTC AGAGGAGCCC CTGGATGGCC AGGCCTGAAA
GGAAGTAAAG GGGAACGAGG CCGCCCAGGA AAGGATGCCA TGGGGACTCC TGGGTCCCCA
GGTTGTGCTG GTTCACCAGG TCTTCCAGGA TCACCGGGAC CTCCAGGACC GCCAGGTGAC
ATCGTTTTTC GCAAGGGTCC ACCTGGAGAT CACGGACTGC CAGGCTATCT AGGGTCTCCA
GGAATCCCAG GAGTTGATGG GCCCAAAGGA GAACCAGGCC TCCTGTGTAC ACAGTGCCCT
TATATCCCAG GGCCTCCCGG TCTCCCAGGA TTGCCAGGGT TACATGGTGT AAAAGGAATC
CCAGGAAGAC AAGGCGCAGC TGGCTTGAAA GGAAGCCCAG GGTCCCCAGG AAATACAGGT
CTTCCAGGAT TTCCAGGTTT CCCAGGTGCC CAGGGTGACC CAGGACTTAA AGGAGAAAAA
GGTGAAACAC TTCAGCCTGA GGGGCAAGTG GGTGTCCCAG GTGACCCGGG GCTCAGAGGC
CAACCTGGGA GAAAGGGCTT GGATGGAATT CCTGGAACTC CGGGAGTGAA AGGATTACCA
GGACCTAAAG GCGAACTGGC TCTGAGTGGT GAGAAAGGGG ACCAAGGTCC TCCAGGGGAT
CCTGGCTCCC CTGGGTCCCC AGGACCTGCA GGACCAGCTG GACCACCTGG CTACGGACCC
CAAGGAGAAC CTGGTCTCCA GGGCACGCAA GGAGTTCCTG GAGCCCCCGG ACCACCCGGA
GAAGCCGGCC CTAGGGGAGA GCTCAGTGTT TCAACACCAG TTCCAGGCCC ACCAGGACCT
CCAGGGCCCC CTGGCCATCC TGGCCCCCAA GGTCCACCTG GTATCCCTGG ATCCCTGGGG
AAATGTGGAG ATCCTGGTCT TCCAGGGCCT GATGGTGAAC CAGGAATTCC AGGAATTGGA
TTTCCTGGGC CTCCTGGACC TAAGGGAGAC CAAGGTTTTC CAGGTACAAA AGGATCACTG
GGTTGTCCTG GAAAAATGGG AGAGCCTGGG TTACCTGGAA AGCCAGGCCT CCCAGGAGCC
AAGGGAGAAC CAGCAGTAGC CATGCCTGGA GGACCAGGAA CACCAGGTTT TCCAGGAGAA
AGAGGCAATT CTGGGGAACA TGGAGAAATT GGACTCCCTG GACTTCCAGG TCTCCCTGGA
ACTCCAGGAA ATGAAGGGCT TGATGGACCA CGAGGAGATC CAGGGCAGCC TGGACCACCT
GGAGAACAAG GACCCCCAGG AAGGTGCATA GAGGGTCCCA GGGGAGCCCA AGGACTTCCA
GGCTTAAATG GATTGAAAGG GCAACAAGGC AGAAGAGGTA AAACGGGGCC AAAGGGAGAC
CCAGGAATTC CAGGCTTGGA TAGATCAGGA TTTCCTGGAG AAACTGGATC ACCAGGAATT
CCAGGTCATC AAGGTGAAAT GGGACCACTG GGTCAAAGAG GATATCCAGG AAATCCGGGA
ATTTTAGGGC CACCAGGTGA AGATGGAGTG ATTGGGATGA TGGGCTTTCC TGGAGCCATT
GGCCCTCCAG GGCCCCCTGG GAACCCAGGC ACACCAGGGC AGAGGGGGAG CCCTGGAATT
CCAGGAGTAA AGGGCCAGAG AGGAACCCCA GGAGCCAAGG GGGAACAAGG AGATAAAGGA
AATCCCGGGC CTTCAGAGAT ATCCCACGTA ATAGGGGACA AAGGAGAACC AGGTCTCAAA
GGATTCGCAG GAAATCCAGG TGAGAAAGGA AACAGAGGCG TTCCAGGGAT GCCAGGTTTA
AAGGGCCTCA AAGGACTACC CGGACCAGCA GGACCACCAG GCCCCAGAGG AGATTTGGGC
AGCACTGGGA ATCCTGGAGA ACCAGGACTG CGTGGTATAC CAGGAAGCAT GGGGAACATG
GGCATGCCAG GTTCTAAAGG AAAAAGGGGA ACTTTGGGAT TCCCAGGTCG AGCAGGAAGA
CCAGGCCTCC CAGGTATTCA TGGTCTCCAG GGAGATAAGG GAGAGCCAGG TTATTCAGAA
GGTACAAGGC CAGGACCACC GGGACCAACG GGGGATCCAG GACTGCCGGG TGATATGGGA
AAGAAAGGAG AAATGGGGCA ACCTGGCCCA CCTGGACATT TGGGGCCTGC TGGACCTGAG
GGAGCCCCTG GAAGTCCTGG AAGTCCTGGC CTCCCAGGAA AGCCAGGTCC TCATGGTGAT
TTGGGTTTTA AAGGAATCAA AGGCCTCCTG GGCCCTCCAG GAATCAGAGG CCCTCCAGGT
CTTCCAGGAT TTCCAGGATC TCCTGGACCA ATGGGTATAA GAGGTGACCA AGGACGTGAT
GGAATTCCTG GTCCAGCCGG AGAAAAGGGA GAAACGGGTT TATTGAGGGC CCCTCCAGGC
CCAAGAGGGA ACCCTGGTGC TCAAGGAGCC AAAGGAGACA GGGGAGCCCC AGGTTTTCCT
GGCCTCCCGG GCAGAAAAGG GGCCATGGGA GATGCTGGAC CTCGAGGACC CACAGGCATA
GAAGGATTCC CAGGGCCACC AGGTCTGCCC GGTGCAATTA TCCCTGGCCA GACAGGAAAT
CGTGGTCCAC CAGGCTCAAG AGGAAGCCCA GGTGCGCCTG GTCCCCCTGG ACCTCCAGGG
AGTCATGTAA TAGGCATAAA AGGAGACAAA GGGTCTATGG GCCACCCTGG CCCAAAAGGT
CCACCTGGAA CTGCAGGAGA CATGGGACCA CCAGGTCGTC TGGGAGCACC AGGTACTCCA
GGTCTTCCAG GACCCAGAGG TGATCCTGGA TTCCAGGGGT TTCCAGGCGT GAAAGGAGAA
AAGGGTAATC CTGGATTTCT AGGATCCATT GGACCTCCAG GACCAATTGG GCCAAAAGGA
CCACCTGGTG TACGTGGAGA CCCTGGCACA CTTAAGATTA TCTCCCTTCC AGGAAGCCCA
GGGCCACCTG GCACACCTGG AGAACCAGGG ATGCAGGGAG AACCTGGGCC ACCAGGGCCA
CCTGGAAACC TAGGACCCTG TGGGCCAAGA GGTAAGCCAG GCAAGGATGG AAAACCAGGA
ACTCCTGGAC CAGCTGGAGA AAAAGGCAAC AAAGGTTCTA AAGGAGAGCC AGGACCAGCT
GGATCAGATG GATTGCCAGG TTTGAAAGGA AAACGTGGAG ACAGTGGATC ACCTGCAACC
TGGACAACGA GAGGCTTTGT CTTCACCCGA CACAGTCAAA CCACAGCAAT TCCTTCATGT
CCAGAGGGGA CAGTGCCACT CTACAGTGGG TTTTCTTTTC TTTTTGTACA AGGAAATCAA
CGAGCCCACG GACAAGACCT TGGAACTCTT GGCAGCTGCC TGCAGCGATT TACCACAATG
CCATTCTTAT TCTGCAATGT CAATGATGTA TGTAATTTTG CATCTCGAAA TGATTATTCA
TACTGGCTGT CAACACCAGC TCTGATGCCA ATGAACATGG CTCCCATTAC TGGCAGAGCC
CTTGAGCCTT ATATAAGCAG ATGCACTGTT TGTGAAGGTC CTGCGATCGC CATAGCCGTT
CACAGCCAAA CCACTGACAT TCCTCCATGT CCTCACGGCT GGATTTCTCT CTGGAAAGGA
TTTTCATTCA TCATGTTCAC AAGTGCAGGT TCTGAGGGCA CCGGGCAAGC ACTGGCCTCC
CCTGGCTCCT GCCTGGAAGA ATTCCGAGCC AGCCCATTTC TAGAATGTCA TGGAAGAGGA
ACGTGCAACT ACTATTCAAA TTCCTACAGT TTCTGGCTGG CTTCATTAAA CCCAGAAAGA
ATGTTCAGAA AGCCTATTCC ATCAACTGTG AAAGCTGGGG AATTAGAAAA AATAATAAGT
CGCTGTCAGG TGTGCATGAA GAAAAGACAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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