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CRYAB cDNA ORF clone, Homo sapiens (human)

Gene Symbol CRYAB
Entrez Gene ID 1410
Full Name crystallin, alpha B
Synonyms CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2
General protein information
Preferred Names
alpha-crystallin B chain
Names
alpha-crystallin B chain
heat shock protein beta-5
rosenthal fiber component
heat-shock 20 kD like-protein
renal carcinoma antigen NY-REN-27
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q23.1

Summary Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]. lac of sum
Disorder MIM:

123590

Disorder Html: Myopathy, cardioskeletal, desmin-related, with cataract, 608810 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001289807 NP_001276736 alpha-crystallin B chain
NM_001885 NP_001876 alpha-crystallin B chain
NM_001289808 NP_001276737 alpha-crystallin B chain
XM_011542608 XP_011540910 alpha-crystallin B chain isoform X1
XM_011542609 XP_011540911 alpha-crystallin B chain isoform X2



Homo sapiens (human) CRYAB NP_001876.1
Pan troglodytes (chimpanzee) CRYAB XP_508752.2
Macaca mulatta (Rhesus monkey) CRYAB XP_002799808.1
Canis lupus familiaris (dog) CRYAB XP_862258.2
Bos taurus (cattle) CRYAB NP_776715.1
Mus musculus (house mouse) Cryab NP_034094.1
Rattus norvegicus (Norway rat) Cryab NP_037067.1
Gallus gallus (chicken) CRYAB NP_990507.1
Danio rerio (zebrafish) cryabb NP_001002670.1
Drosophila melanogaster (fruit fly) l(2)efl NP_523827.1
Xenopus (Silurana) tropicalis (western clawed frog) cryab XP_002932964.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CRYAB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYAB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu16676
NM_001289807 Homo sapiens crystallin, alpha B (CRYAB), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16676
NM_001885 Homo sapiens crystallin, alpha B (CRYAB), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16676
NM_001289808 Homo sapiens crystallin, alpha B (CRYAB), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu16676
XM_011542608 PREDICTED: Homo sapiens crystallin, alpha B (CRYAB), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu57086 XM_011542609 PREDICTED: Homo sapiens crystallin, alpha B (CRYAB), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16676
Clone ID Related Accession (Same CDS sequence) NM_001289807 , NM_001289808 , NM_001885 , XM_011542608
Accession Version NM_001289807.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUL-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB506606.1, BI759672.1, BM721803.1, BC007008.1 and BF339748.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI759672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
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181
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361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276736.1
CDS274..801
Misc Feature(1)217..219(+)
Misc Feature(2)274..450(+)
Misc Feature(3)274..276(+)
Misc Feature(4)304..756(+)
Misc Feature(5)328..330(+)
Misc Feature(6)406..408(+)
Misc Feature(7)415..417(+)
Misc Feature(8)448..450(+)
Misc Feature(9)451..453(+)
Misc Feature(10)472..723(+)
Misc Feature(11)475..699(+)
Misc Feature(12)475..477(+)
Misc Feature(13)547..549(+)
Misc Feature(14)769..771(+)
Exon (1)1..75
Gene:CRYAB
Gene Synonym:
Exon (2)76..474
Gene:CRYAB
Gene Synonym:
Exon (3)475..597
Gene:CRYAB
Gene Synonym:
Exon (4)598..945
Gene:CRYAB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001289807
Organism Homo sapiens (human)
Definition Homo sapiens crystallin, alpha B (CRYAB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289807

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16676
Clone ID Related Accession (Same CDS sequence) NM_001289807 , NM_001289808 , NM_001885 , XM_011542608
Accession Version NM_001885.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUL-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB506606.1, BM721803.1, BC007008.1 and BF339748.1. This sequence is a reference standard in the RefSeqGene project. On Jan 31, 2014 this sequence version replaced gi:4503056. Transcript Variant: This variant (1) represents the longest transcript. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG750124.1, CF127116.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962343 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001876.1
CDS308..835
Misc Feature(1)251..253(+)
Misc Feature(2)308..484(+)
Misc Feature(3)308..310(+)
Misc Feature(4)338..790(+)
Misc Feature(5)362..364(+)
Misc Feature(6)362..364(+)
Misc Feature(7)368..370(+)
Misc Feature(8)371..373(+)
Misc Feature(9)434..436(+)
Misc Feature(10)440..442(+)
Misc Feature(11)440..442(+)
Misc Feature(12)449..451(+)
Misc Feature(13)455..457(+)
Misc Feature(14)464..466(+)
Misc Feature(15)482..484(+)
Misc Feature(16)482..484(+)
Misc Feature(17)485..487(+)
Misc Feature(18)506..757(+)
Misc Feature(19)509..733(+)
Misc Feature(20)509..511(+)
Misc Feature(21)533..535(+)
Misc Feature(22)551..553(+)
Misc Feature(23)581..583(+)
Misc Feature(24)581..583(+)
Misc Feature(25)803..805(+)
Misc Feature(26)803..805(+)
Misc Feature(27)815..817(+)
Misc Feature(28)827..829(+)
Misc Feature(29)830..832(+)
Exon (1)1..109
Gene:CRYAB
Gene Synonym:
Exon (2)110..508
Gene:CRYAB
Gene Synonym:
Exon (3)509..631
Gene:CRYAB
Gene Synonym:
Exon (4)632..979
Gene:CRYAB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001885
Organism Homo sapiens (human)
Definition Homo sapiens crystallin, alpha B (CRYAB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001885

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16676
Clone ID Related Accession (Same CDS sequence) NM_001289807 , NM_001289808 , NM_001885 , XM_011542608
Accession Version NM_001289808.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUL-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI548180.1, BE076509.1, BM721803.1, BC007008.1 and BF339748.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DT217536.1, BM926107.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
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361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276737.1
CDS303..830
Misc Feature(1)246..248(+)
Misc Feature(2)303..479(+)
Misc Feature(3)303..305(+)
Misc Feature(4)333..785(+)
Misc Feature(5)357..359(+)
Misc Feature(6)435..437(+)
Misc Feature(7)444..446(+)
Misc Feature(8)477..479(+)
Misc Feature(9)480..482(+)
Misc Feature(10)501..752(+)
Misc Feature(11)504..728(+)
Misc Feature(12)504..506(+)
Misc Feature(13)576..578(+)
Misc Feature(14)798..800(+)
Exon (1)1..503
Gene:CRYAB
Gene Synonym:
Exon (2)504..626
Gene:CRYAB
Gene Synonym:
Exon (3)627..974
Gene:CRYAB
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001289808
Organism Homo sapiens (human)
Definition Homo sapiens crystallin, alpha B (CRYAB), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289808

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16676
Clone ID Related Accession (Same CDS sequence) NM_001289807 , NM_001289808 , NM_001885 , XM_011542608
Accession Version XM_011542608.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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361
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481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011540910.1
CDS289..816
Misc Feature(1)289..465(+)
Misc Feature(2)319..771(+)
Misc Feature(3)487..738(+)
Misc Feature(4)490..714(+)
Translation

Target ORF information:

RefSeq Version XM_011542608
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens crystallin, alpha B (CRYAB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011542608

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57086
Clone ID Related Accession (Same CDS sequence) XM_011542609
Accession Version XM_011542609.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 327bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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121
181
241
301
ATGCGCCTGG AGAAGGACAG GTTCTCTGTC AACCTGGATG TGAAGCACTT CTCCCCAGAG 
GAACTCAAAG TTAAGGTGTT GGGAGATGTG ATTGAGGTGC ATGGAAAACA TGAAGAGCGC
CAGGATGAAC ATGGTTTCAT CTCCAGGGAG TTCCACAGGA AATACCGGAT CCCAGCTGAT
GTAGACCCTC TCACCATTAC TTCATCCCTG TCATCTGATG GGGTCCTCAC TGTGAATGGA
CCAAGGAAAC AGGTCTCTGG CCCTGAGCGC ACCATTCCCA TCACCCGTGA AGAGAAGCCT
GCTGTCACCG CAGCCCCCAA GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011540911.1
CDS665..991
Misc Feature(1)665..913(+)
Misc Feature(2)680..946(+)
Translation

Target ORF information:

RefSeq Version XM_011542609
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens crystallin, alpha B (CRYAB), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011542609

ORF Insert Sequence:

1
61
121
181
241
301
ATGCGCCTGG AGAAGGACAG GTTCTCTGTC AACCTGGATG TGAAGCACTT CTCCCCAGAG 
GAACTCAAAG TTAAGGTGTT GGGAGATGTG ATTGAGGTGC ATGGAAAACA TGAAGAGCGC
CAGGATGAAC ATGGTTTCAT CTCCAGGGAG TTCCACAGGA AATACCGGAT CCCAGCTGAT
GTAGACCCTC TCACCATTAC TTCATCCCTG TCATCTGATG GGGTCCTCAC TGTGAATGGA
CCAAGGAAAC AGGTCTCTGG CCCTGAGCGC ACCATTCCCA TCACCCGTGA AGAGAAGCCT
GCTGTCACCG CAGCCCCCAA GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Substantially elevating the levels of alphaB-crystallin in spinal motor neurons of mutant SOD1 mice does not significantly delay paralysis or attenuate mutant protein aggregation
J. Neurochem. 133 (3), 452-464 (2015)
Xu G, Fromholt S, Ayers JI, Brown H, Siemienski Z, Crosby KW, Mayer CA, Janus C and Borchelt DR.


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Alpha B- and betaA3-crystallins containing d-aspartic acids exist in a monomeric state
Biochim. Biophys. Acta 1854 (1), 1-9 (2015)
Sakaue H, Takata T, Fujii N, Sasaki H and Fujii N.


book

Prevention of aberrant protein aggregation by anchoring the molecular chaperone alphaB-crystallin to the endoplasmic reticulum
Biochem. Biophys. Res. Commun. 455 (3-4), 241-245 (2014)
Yamamoto S, Yamashita A, Arakaki N, Nemoto H and Yamazaki T.


book

Alpha B-crystallin correlates with poor survival in colorectal cancer
Int J Clin Exp Pathol 7 (9), 6056-6063 (2014)
Shi C, He Z, Hou N, Ni Y, Xiong L and Chen P.


book

Alpha-crystallin as a molecular chaperone
Prog Retin Eye Res 18 (4), 463-509 (1999)
Derham BK and Harding JJ.


book

Myofibrillar Myopathy
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Selcen,D. and Engel,A.G.


book

Site-specific glycation of lens crystallins by ascorbic acid
Biochim. Biophys. Acta 1117 (2), 207-215 (1992)
Ortwerth BJ, Slight SH, Prabhakaram M, Sun Y and Smith JB.


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Accumulation of alpha B-crystallin in brains of patients with Alexander's disease is not due to an abnormality of the 5'-flanking and coding sequence of the genomic DNA
Neurosci. Lett. 140 (1), 89-92 (1992)
Iwaki A, Iwaki T, Goldman JE, Ogomori K, Tateishi J and Sakaki Y.


book

Copurification of small heat shock protein with alpha B crystallin from human skeletal muscle
J. Biol. Chem. 267 (11), 7718-7725 (1992)
Kato K, Shinohara H, Goto S, Inaguma Y, Morishita R and Asano T.


book

The primary structure of the B2 chain of human alpha-crystallin
FEBS Lett. 74 (1), 82-84 (1977)
Kramps,J.A., de Man,B.M. and de Jong,W.W.


 
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