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CRYAB crystallin, alpha B [Homo sapiens (human)]

Gene Symbol CRYAB
Entrez Gene ID 1410
Full Name crystallin, alpha B
Synonyms CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2
General protein information
Preferred Names
alpha-crystallin B chain
Names
alpha-crystallin B chain
heat shock protein beta-5
rosenthal fiber component
heat-shock 20 kD like-protein
renal carcinoma antigen NY-REN-27
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q23.1

Summary Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]. lac of sum
Disorder MIM:

123590

Disorder Html: Myopathy, cardioskeletal, desmin-related, with cataract, 608810 (3);
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu16676 NM_001289807 Homo sapiens crystallin, alpha B (CRYAB), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu16676 NM_001885 Homo sapiens crystallin, alpha B (CRYAB), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu16676 NM_001289808 Homo sapiens crystallin, alpha B (CRYAB), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu16676 XM_011542608 PREDICTED: Homo sapiens crystallin, alpha B (CRYAB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OHu57086 XM_011542609 PREDICTED: Homo sapiens crystallin, alpha B (CRYAB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu16676D
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUL-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB506606.1, BI759672.1, BM721803.1, BC007008.1 and BF339748.1. Summary: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI759672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001276736.1
CDS274..801
Misc Feature(1)217..219(+)
Misc Feature(2)274..450(+)
Misc Feature(3)274..276(+)
Misc Feature(4)304..756(+)
Misc Feature(5)328..330(+)
Misc Feature(6)406..408(+)
Misc Feature(7)415..417(+)
Misc Feature(8)448..450(+)
Misc Feature(9)451..453(+)
Misc Feature(10)472..723(+)
Misc Feature(11)475..699(+)
Misc Feature(12)475..477(+)
Misc Feature(13)547..549(+)
Misc Feature(14)769..771(+)
Exon (1)1..75
Gene:CRYAB
Gene Synonym:
Exon (2)76..474
Gene:CRYAB
Gene Synonym:
Exon (3)475..597
Gene:CRYAB
Gene Synonym:
Exon (4)598..945
Gene:CRYAB
Gene Synonym:
Translation
Position Chain Variation Link
17 17 a, g dbSNP:760785788
27 27 g, t dbSNP:57653928
276 276 a, g dbSNP:397516686
291 291 c, t dbSNP:529878363
331 331 c, t dbSNP:387907337
333 333 -, c dbSNP:281865141
438 438 a, c, g dbSNP:2228387
439 439 c, t dbSNP:387907338
598 598 c, g dbSNP:387907339
616 616 -, t dbSNP:281865142
631 631 a, g dbSNP:104894201
691 691 a, g dbSNP:387907336
724 724 c, t dbSNP:104894202
733 733 a, g dbSNP:150516929
743 743 a, g dbSNP:141638421
758 758 c, t dbSNP:199861008
937 937 a, g, t dbSNP:112204677

Target ORF information:

RefSeq Version NM_001289807
Organism Homo sapiens (human)
Definition Homo sapiens crystallin, alpha B (CRYAB), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001289807

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu16676D
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUL-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB506606.1, BM721803.1, BC007008.1 and BF339748.1. This sequence is a reference standard in the RefSeqGene project. On Jan 31, 2014 this sequence version replaced gi:4503056. Summary: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]. Transcript Variant: This variant (1) represents the longest transcript. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG750124.1, CF127116.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962343 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001876.1
CDS308..835
Misc Feature(1)251..253(+)
Misc Feature(2)308..484(+)
Misc Feature(3)308..310(+)
Misc Feature(4)338..790(+)
Misc Feature(5)362..364(+)
Misc Feature(6)362..364(+)
Misc Feature(7)368..370(+)
Misc Feature(8)371..373(+)
Misc Feature(9)434..436(+)
Misc Feature(10)440..442(+)
Misc Feature(11)440..442(+)
Misc Feature(12)449..451(+)
Misc Feature(13)455..457(+)
Misc Feature(14)464..466(+)
Misc Feature(15)482..484(+)
Misc Feature(16)482..484(+)
Misc Feature(17)485..487(+)
Misc Feature(18)506..757(+)
Misc Feature(19)509..733(+)
Misc Feature(20)509..511(+)
Misc Feature(21)533..535(+)
Misc Feature(22)551..553(+)
Misc Feature(23)581..583(+)
Misc Feature(24)581..583(+)
Misc Feature(25)803..805(+)
Misc Feature(26)803..805(+)
Misc Feature(27)815..817(+)
Misc Feature(28)827..829(+)
Misc Feature(29)830..832(+)
Exon (1)1..109
Gene:CRYAB
Gene Synonym:
Exon (2)110..508
Gene:CRYAB
Gene Synonym:
Exon (3)509..631
Gene:CRYAB
Gene Synonym:
Exon (4)632..979
Gene:CRYAB
Gene Synonym:
Translation
Position Chain Variation Link
17 17 a, g dbSNP:760785788
27 27 g, t dbSNP:57653928
102 102 a, g dbSNP:773886667
310 310 a, g dbSNP:397516686
325 325 c, t dbSNP:529878363
365 365 c, t dbSNP:387907337
367 367 -, c dbSNP:281865141
472 472 a, c, g dbSNP:2228387
473 473 c, t dbSNP:387907338
632 632 c, g dbSNP:387907339
650 650 -, t dbSNP:281865142
665 665 a, g dbSNP:104894201
725 725 a, g dbSNP:387907336
758 758 c, t dbSNP:104894202
767 767 a, g dbSNP:150516929
777 777 a, g dbSNP:141638421
792 792 c, t dbSNP:199861008
971 971 a, g, t dbSNP:112204677

Target ORF information:

RefSeq Version NM_001885
Organism Homo sapiens (human)
Definition Homo sapiens crystallin, alpha B (CRYAB), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001885

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu16676D
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUL-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI548180.1, BE076509.1, BM721803.1, BC007008.1 and BF339748.1. Summary: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DT217536.1, BM926107.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 10217480 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001276737.1
CDS303..830
Misc Feature(1)246..248(+)
Misc Feature(2)303..479(+)
Misc Feature(3)303..305(+)
Misc Feature(4)333..785(+)
Misc Feature(5)357..359(+)
Misc Feature(6)435..437(+)
Misc Feature(7)444..446(+)
Misc Feature(8)477..479(+)
Misc Feature(9)480..482(+)
Misc Feature(10)501..752(+)
Misc Feature(11)504..728(+)
Misc Feature(12)504..506(+)
Misc Feature(13)576..578(+)
Misc Feature(14)798..800(+)
Exon (1)1..503
Gene:CRYAB
Gene Synonym:
Exon (2)504..626
Gene:CRYAB
Gene Synonym:
Exon (3)627..974
Gene:CRYAB
Gene Synonym:
Translation
Position Chain Variation Link
54 54 c, g dbSNP:14133
305 305 a, g dbSNP:397516686
320 320 c, t dbSNP:529878363
360 360 c, t dbSNP:387907337
362 362 -, c dbSNP:281865141
467 467 a, c, g dbSNP:2228387
468 468 c, t dbSNP:387907338
627 627 c, g dbSNP:387907339
645 645 -, t dbSNP:281865142
660 660 a, g dbSNP:104894201
720 720 a, g dbSNP:387907336
753 753 c, t dbSNP:104894202
762 762 a, g dbSNP:150516929
772 772 a, g dbSNP:141638421
787 787 c, t dbSNP:199861008
966 966 a, g, t dbSNP:112204677

Target ORF information:

RefSeq Version NM_001289808
Organism Homo sapiens (human)
Definition Homo sapiens crystallin, alpha B (CRYAB), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001289808

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu16676D
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011540910.1
CDS289..816
Misc Feature(1)289..465(+)
Misc Feature(2)319..771(+)
Misc Feature(3)487..738(+)
Misc Feature(4)490..714(+)
Translation
Position Chain Variation Link
74 74 c, t dbSNP:111577414
291 291 a, g dbSNP:397516686
306 306 c, t dbSNP:529878363
346 346 c, t dbSNP:387907337
348 348 -, c dbSNP:281865141
453 453 a, c, g dbSNP:2228387
454 454 c, t dbSNP:387907338
613 613 c, g dbSNP:387907339
631 631 -, t dbSNP:281865142
646 646 a, g dbSNP:104894201
706 706 a, g dbSNP:387907336
739 739 c, t dbSNP:104894202
748 748 a, g dbSNP:150516929
758 758 a, g dbSNP:141638421
773 773 c, t dbSNP:199861008
952 952 a, g, t dbSNP:112204677

Target ORF information:

RefSeq Version XM_011542608
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens crystallin, alpha B (CRYAB), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011542608

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGACATCG CCATCCACCA CCCCTGGATC CGCCGCCCCT TCTTTCCTTT CCACTCCCCC 
AGCCGCCTCT TTGACCAGTT CTTCGGAGAG CACCTGTTGG AGTCTGATCT TTTCCCGACG
TCTACTTCCC TGAGTCCCTT CTACCTTCGG CCACCCTCCT TCCTGCGGGC ACCCAGCTGG
TTTGACACTG GACTCTCAGA GATGCGCCTG GAGAAGGACA GGTTCTCTGT CAACCTGGAT
GTGAAGCACT TCTCCCCAGA GGAACTCAAA GTTAAGGTGT TGGGAGATGT GATTGAGGTG
CATGGAAAAC ATGAAGAGCG CCAGGATGAA CATGGTTTCA TCTCCAGGGA GTTCCACAGG
AAATACCGGA TCCCAGCTGA TGTAGACCCT CTCACCATTA CTTCATCCCT GTCATCTGAT
GGGGTCCTCA CTGTGAATGG ACCAAGGAAA CAGGTCTCTG GCCCTGAGCG CACCATTCCC
ATCACCCGTG AAGAGAAGCC TGCTGTCACC GCAGCCCCCA AGAAATAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu57086D
Sequence Information ORF Nucleotide Sequence (Length: 327bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product alpha-crystallin B chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_033899.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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ATGCGCCTGG AGAAGGACAG GTTCTCTGTC AACCTGGATG TGAAGCACTT CTCCCCAGAG 
GAACTCAAAG TTAAGGTGTT GGGAGATGTG ATTGAGGTGC ATGGAAAACA TGAAGAGCGC
CAGGATGAAC ATGGTTTCAT CTCCAGGGAG TTCCACAGGA AATACCGGAT CCCAGCTGAT
GTAGACCCTC TCACCATTAC TTCATCCCTG TCATCTGATG GGGTCCTCAC TGTGAATGGA
CCAAGGAAAC AGGTCTCTGG CCCTGAGCGC ACCATTCCCA TCACCCGTGA AGAGAAGCCT
GCTGTCACCG CAGCCCCCAA GAAATAG
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_011540911.1
CDS665..991
Misc Feature(1)665..913(+)
Misc Feature(2)680..946(+)
Translation
Position Chain Variation Link
460 460 a, g dbSNP:188986241
788 788 c, g dbSNP:387907339
806 806 -, t dbSNP:281865142
821 821 a, g dbSNP:104894201
881 881 a, g dbSNP:387907336
914 914 c, t dbSNP:104894202
923 923 a, g dbSNP:150516929
933 933 a, g dbSNP:141638421
948 948 c, t dbSNP:199861008
1127 1127 a, g, t dbSNP:112204677

Target ORF information:

RefSeq Version XM_011542609
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens crystallin, alpha B (CRYAB), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_011542609

ORF Insert Sequence:

1
61
121
181
241
301
ATGCGCCTGG AGAAGGACAG GTTCTCTGTC AACCTGGATG TGAAGCACTT CTCCCCAGAG 
GAACTCAAAG TTAAGGTGTT GGGAGATGTG ATTGAGGTGC ATGGAAAACA TGAAGAGCGC
CAGGATGAAC ATGGTTTCAT CTCCAGGGAG TTCCACAGGA AATACCGGAT CCCAGCTGAT
GTAGACCCTC TCACCATTAC TTCATCCCTG TCATCTGATG GGGTCCTCAC TGTGAATGGA
CCAAGGAAAC AGGTCTCTGG CCCTGAGCGC ACCATTCCCA TCACCCGTGA AGAGAAGCCT
GCTGTCACCG CAGCCCCCAA GAAATAG

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.