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PRICKLE1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol PRICKLE1
Entrez Gene ID 144165
Full Name prickle homolog 1
Synonyms EPM1B, RILP
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q12

Summary This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009]. lac of sum
Disorder MIM:

608500

Disorder Html: Epilepsy, progressive myoclonic 1B, 612437 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001144881 NP_001138353 prickle-like protein 1
NM_153026 NP_694571 prickle-like protein 1
NM_001144883 NP_001138355 prickle-like protein 1
NM_001144882 NP_001138354 prickle-like protein 1
XM_011537946 XP_011536248 prickle-like protein 1 isoform X1
XM_011537947 XP_011536249 prickle-like protein 1 isoform X1



Homo sapiens (human) PRICKLE1 NP_001138355.1
Pan troglodytes (chimpanzee) PRICKLE1 XP_001166831.1
Macaca mulatta (Rhesus monkey) PRICKLE1 XP_002798581.1
Canis lupus familiaris (dog) PRICKLE1 XP_005637023.1
Bos taurus (cattle) PRICKLE1 NP_001096004.1
Mus musculus (house mouse) Prickle1 NP_001028389.1
Rattus norvegicus (Norway rat) Prickle1 NP_955428.1
Gallus gallus (chicken) PRICKLE1 XP_416036.2
Danio rerio (zebrafish) prickle1a NP_899185.2
Xenopus (Silurana) tropicalis (western clawed frog) prickle1 NP_001016939.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRICKLE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRICKLE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu22315 NM_001144881 Homo sapiens prickle homolog 1 (PRICKLE1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 NM_153026 Homo sapiens prickle homolog 1 (PRICKLE1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 NM_001144883 Homo sapiens prickle homolog 1 (PRICKLE1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 NM_001144882 Homo sapiens prickle homolog 1 (PRICKLE1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu22315 XM_011537946 PREDICTED: Homo sapiens prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
OHu22315 XM_011537947 PREDICTED: Homo sapiens prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144881 , NM_001144882 , NM_001144883 , NM_153026 , XM_011537946 , XM_011537947
Accession Version NM_001144881.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 24-JUL-2015
Organism Homo sapiens (human)
Product prickle-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK122877.1, AC079601.25, BC114939.1 and BE858932.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138353.1
CDS288..2783
Misc Feature(1)261..263(+)
Misc Feature(2)351..641(+)
Misc Feature(3)663..839(+)
Misc Feature(4)663..833(+)
Misc Feature(5)852..1019(+)
Misc Feature(6)858..1013(+)
Misc Feature(7)1032..1208(+)
Misc Feature(8)1038..1205(+)
Misc Feature(9)2769..2771(+)
Exon (1)1..239
Gene:PRICKLE1
Gene Synonym:
Exon (2)240..419
Gene:PRICKLE1
Gene Synonym:
Exon (3)420..533
Gene:PRICKLE1
Gene Synonym:
Exon (4)534..671
Gene:PRICKLE1
Gene Synonym:
Exon (5)672..875
Gene:PRICKLE1
Gene Synonym:
Exon (6)876..1062
Gene:PRICKLE1
Gene Synonym:
Exon (7)1063..1926
Gene:PRICKLE1
Gene Synonym:
Exon (8)1927..4254
Gene:PRICKLE1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001144881
Organism Homo sapiens (human)
Definition Homo sapiens prickle homolog 1 (PRICKLE1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001144881

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144881 , NM_001144882 , NM_001144883 , NM_153026 , XM_011537946 , XM_011537947
Accession Version NM_153026.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 24-JUL-2015
Organism Homo sapiens (human)
Product prickle-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA413584.1, BC114939.1, AC079601.25 and BE858932.1. This sequence is a reference standard in the RefSeqGene project. On or before Jan 30, 2009 this sequence version replaced gi:169204134, gi:169204694, gi:23308518. Transcript Variant: This variant (1) represents the longest transcript. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF399844.1, AK056499.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_694571.2
CDS355..2850
Misc Feature(1)328..330(+)
Misc Feature(2)418..708(+)
Misc Feature(3)730..906(+)
Misc Feature(4)730..900(+)
Misc Feature(5)919..1086(+)
Misc Feature(6)925..1080(+)
Misc Feature(7)1099..1275(+)
Misc Feature(8)1105..1272(+)
Misc Feature(9)2836..2838(+)
Exon (1)1..306
Gene:PRICKLE1
Gene Synonym:
Exon (2)307..486
Gene:PRICKLE1
Gene Synonym:
Exon (3)487..600
Gene:PRICKLE1
Gene Synonym:
Exon (4)601..738
Gene:PRICKLE1
Gene Synonym:
Exon (5)739..942
Gene:PRICKLE1
Gene Synonym:
Exon (6)943..1129
Gene:PRICKLE1
Gene Synonym:
Exon (7)1130..1993
Gene:PRICKLE1
Gene Synonym:
Exon (8)1994..4321
Gene:PRICKLE1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_153026
Organism Homo sapiens (human)
Definition Homo sapiens prickle homolog 1 (PRICKLE1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153026

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144881 , NM_001144882 , NM_001144883 , NM_153026 , XM_011537946 , XM_011537947
Accession Version NM_001144883.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 24-JUL-2015
Organism Homo sapiens (human)
Product prickle-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA321918.1, AK122881.1, CN390789.1, AK056189.1, AC079601.25 and BE858932.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122881.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138355.1
CDS284..2779
Misc Feature(1)257..259(+)
Misc Feature(2)347..637(+)
Misc Feature(3)659..835(+)
Misc Feature(4)659..829(+)
Misc Feature(5)848..1015(+)
Misc Feature(6)854..1009(+)
Misc Feature(7)1028..1204(+)
Misc Feature(8)1034..1201(+)
Misc Feature(9)2765..2767(+)
Exon (1)1..238
Gene:PRICKLE1
Gene Synonym:
Exon (2)239..415
Gene:PRICKLE1
Gene Synonym:
Exon (3)416..529
Gene:PRICKLE1
Gene Synonym:
Exon (4)530..667
Gene:PRICKLE1
Gene Synonym:
Exon (5)668..871
Gene:PRICKLE1
Gene Synonym:
Exon (6)872..1058
Gene:PRICKLE1
Gene Synonym:
Exon (7)1059..1922
Gene:PRICKLE1
Gene Synonym:
Exon (8)1923..4250
Gene:PRICKLE1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001144883
Organism Homo sapiens (human)
Definition Homo sapiens prickle homolog 1 (PRICKLE1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001144883

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144881 , NM_001144882 , NM_001144883 , NM_153026 , XM_011537946 , XM_011537947
Accession Version NM_001144882.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 24-JUL-2015
Organism Homo sapiens (human)
Product prickle-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA321918.1, BC114940.1, AC079601.25 and BE858932.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF399844.1, AK122881.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138354.1
CDS287..2782
Misc Feature(1)260..262(+)
Misc Feature(2)350..640(+)
Misc Feature(3)662..838(+)
Misc Feature(4)662..832(+)
Misc Feature(5)851..1018(+)
Misc Feature(6)857..1012(+)
Misc Feature(7)1031..1207(+)
Misc Feature(8)1037..1204(+)
Misc Feature(9)2768..2770(+)
Exon (1)1..238
Gene:PRICKLE1
Gene Synonym:
Exon (2)239..418
Gene:PRICKLE1
Gene Synonym:
Exon (3)419..532
Gene:PRICKLE1
Gene Synonym:
Exon (4)533..670
Gene:PRICKLE1
Gene Synonym:
Exon (5)671..874
Gene:PRICKLE1
Gene Synonym:
Exon (6)875..1061
Gene:PRICKLE1
Gene Synonym:
Exon (7)1062..1925
Gene:PRICKLE1
Gene Synonym:
Exon (8)1926..4253
Gene:PRICKLE1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001144882
Organism Homo sapiens (human)
Definition Homo sapiens prickle homolog 1 (PRICKLE1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001144882

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144881 , NM_001144882 , NM_001144883 , NM_153026 , XM_011537946 , XM_011537947
Accession Version XM_011537946.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product prickle-like protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536248.1
CDS106..2601
Misc Feature(1)169..459(+)
Misc Feature(2)481..657(+)
Misc Feature(3)481..651(+)
Misc Feature(4)670..837(+)
Misc Feature(5)676..831(+)
Misc Feature(6)850..1026(+)
Misc Feature(7)856..1023(+)
Translation

Target ORF information:

RefSeq Version XM_011537946
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011537946

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22315
Clone ID Related Accession (Same CDS sequence) NM_001144881 , NM_001144882 , NM_001144883 , NM_153026 , XM_011537946 , XM_011537947
Accession Version XM_011537947.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product prickle-like protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536249.1
CDS326..2821
Misc Feature(1)389..679(+)
Misc Feature(2)701..877(+)
Misc Feature(3)701..871(+)
Misc Feature(4)890..1057(+)
Misc Feature(5)896..1051(+)
Misc Feature(6)1070..1246(+)
Misc Feature(7)1076..1243(+)
Translation

Target ORF information:

RefSeq Version XM_011537947
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011537947

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGCCTTTGG AGATGGAGCC CAAGATGAGC AAACTGGCCT TTGGCTGTCA GAGAAGTTCC 
ACATCAGATG ATGACTCTGG CTGTGCATTG GAGGAGTACG CCTGGGTCCC CCCGGGCCTG
AGACCAGAGC AGATCCAGCT CTATTTTGCT TGCTTACCAG AGGAAAAAGT TCCTTACGTT
AACAGCCCCG GAGAGAAGCA TCGGATTAAA CAGCTTTTGT ACCAGTTACC ACCACATGAT
AATGAGGTAC GGTATTGCCA GTCTTTGAGT GAAGAGGAGA AAAAAGAGTT GCAGGTGTTC
AGTGCTCAGC GGAAGAAAGA AGCACTGGGA AGAGGAACAA TTAAGCTTCT GTCCAGAGCA
GTCATGCATG CTGTGTGTGA GCAGTGTGGT TTGAAGATAA ATGGAGGTGA AGTTGCAGTG
TTCGCCTCCC GTGCGGGCCC TGGTGTGTGC TGGCACCCAT CCTGTTTTGT CTGTTTCACG
TGTAATGAGC TGCTGGTCGA CCTCATCTAT TTTTATCAGG ATGGAAAAAT TCACTGTGGC
AGGCACCATG CAGAACTGCT CAAACCACGG TGCTCAGCAT GTGACGAGAT AATTTTTGCT
GATGAGTGCA CAGAAGCTGA GGGTCGCCAT TGGCACATGA AACACTTCTG CTGCCTTGAG
TGTGAAACGG TCCTGGGAGG ACAGAGGTAT ATCATGAAGG ACGGCCGCCC CTTCTGCTGT
GGCTGTTTTG AGTCTCTCTA TGCGGAGTAC TGTGAAACCT GTGGGGAACA TATTGGTGTG
GACCATGCAC AGATGACCTA TGACGGGCAG CACTGGCACG CCACGGAAGC CTGCTTTTCT
TGTGCCCAGT GTAAAGCCTC TTTGTTGGGA TGTCCCTTCC TTCCCAAACA GGGTCAGATT
TACTGCTCAA AAACGTGCAG TCTTGGTGAA GACGTCCATG CCTCTGATTC TTCCGACTCT
GCATTTCAGT CAGCTCGATC AAGAGACTCC CGAAGAAGTG TCCGAATGGG CAAGAGCAGC
CGGTCAGCAG ATCAGTGTAG ACAGTCTCTC CTCTTATCGC CTGCTCTGAA CTACAAGTTT
CCTGGCCTCT CAGGCAATGC TGATGACACC CTTTCTCGAA AATTGGATGA TCTGAGTCTC
TCCAGACAAG GAACAAGTTT TGCCAGTGAA GAATTTTGGA AAGGCAGAGT AGAGCAGGAA
ACTCCAGAAG ACCCTGAAGA ATGGGCTGAT CATGAAGATT ATATGACGCA GCTCCTCCTC
AAGTTTGGTG ATAAAAGCCT CTTTCAGCCA CAGCCCAATG AGATGGATAT TCGAGCCAGT
GAGCACTGGA TATCTGATAA CATGGTTAAA AGTAAGACCG AGTTAAAGCA AAATAACCAG
AGCCTTGCAA GTAAAAAATA CCAGTCTGAT ATGTACTGGG CACAGTCACA AGATGGACTG
GGCGATTCTG CTTATGGCAG CCACCCAGGC CCTGCAAGCA GTAGAAGGCT TCAGGAATTG
GAACTGGACC ATGGGGCTTC AGGGTATAAT CATGATGAAA CACAGTGGTA TGAAGATTCC
CTGGAGTGTC TGTCAGACCT GAAACCAGAG CAAAGTGTTC GGGATTCGAT GGATTCTTTG
GCATTGTCCA ATATCACAGG GGCTTCGGTG GATGGAGAAA ACAAGCCAAG GCCATCATTG
TATTCTCTGC AAAATTTTGA GGAGATGGAA ACAGAAGATT GTGAGAAGAT GAGCAATATG
GGAACTTTGA ACTCTTCCAT GCTGCACAGG AGTGCAGAGT CCTTAAAGAG TCTAAGTTCA
GAGTTGTGTC CAGAGAAAAT CCTGCCTGAA GAGAAGCCAG TACATCTGCC AGTGCTCAGA
AGGTCCAAGT CTCAATCCAG ACCCCAGCAG GTCAAGTTTT CTGATGATGT CATTGACAAT
GGGAACTATG ACATTGAAAT CCGGCAGCCT CCGATGAGTG AAAGGACTCG GAGACGCGTC
TACAATTTTG AAGAGAGGGG ATCCAGGTCT CATCACCACC GCCGCCGGAG AAGTAGAAAG
TCCCGCTCCG ACAATGCCCT GAATCTTGTT ACAGAAAGAA AATACTCTCC CAAGGACAGA
CTGCGGCTGT ACACCCCCGA TAACTATGAG AAATTTATAC AGAATAAAAG TGCCCGGGAG
ATCCAAGCAT ACATCCAGAA TGCTGATCTC TACGGACAGT ACGCCCATGC CACTTCCGAT
TATGGCCTGC AGAACCCAGG AATGAATCGG TTTCTGGGAC TCTACGGCGA GGATGATGAT
TCCTGGTGTT CTTCCTCCTC CTCCTCTTCC GACTCGGAAG AAGAAGGATA TTTTCTTGGA
CAACCAATCC CTCAACCCCG GCCACAGAGA TTTGCCTACT ATACAGATGA CCTTTCTAGT
CCACCATCTG CACTTCCCAC CCCTCAGTTT GGTCAGAGGA CAACAAAATC CAAGAAGAAA
AAGGGACACA AGGGCAAAAA TTGTATTATT TCTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


book

PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders
PLoS ONE 8 (12), E80737 (2013)
Paemka L, Mahajan VB, Skeie JM, Sowers LP, Ehaideb SN, Gonzalez-Alegre P, Sasaoka T, Tao H, Miyagi A, Ueno N, Takao K, Miyakawa T, Wu S, Darbro BW, Ferguson PJ, Pieper AA, Britt JK, Wemmie JA, Rudd DS, Wassink T, El-Shanti H, Mefford HC, Carvill GL, Manak JR and Bassuk AG.


book

Mink1 regulates beta-catenin-independent Wnt signaling via Prickle phosphorylation
Mol. Cell. Biol. 32 (1), 173-185 (2012)
Daulat AM, Luu O, Sing A, Zhang L, Wrana JL, McNeill H, Winklbauer R and Angers S.


book

Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women
PLoS Genet. 8 (5), E1002695 (2012)
Fox CS, Liu Y, White CC, Feitosa M, Smith AV, Heard-Costa N, Lohman K, Johnson AD, Foster MC, Greenawalt DM, Griffin P, Ding J, Newman AB, Tylavsky F, Miljkovic I, Kritchevsky SB, Launer L, Garcia M, Eiriksdottir G, Carr JJ, Gudnason V, Harris TB, Cupples LA and Borecki IB.


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Prickle-1 negatively regulates Wnt/beta-catenin pathway by promoting Dishevelled ubiquitination/degradation in liver cancer
Gastroenterology 131 (4), 1218-1227 (2006)
Chan DW, Chan CY, Yam JW, Ching YP and Ng IO.


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Regulation of human tyrosine hydroxylase gene by neuron-restrictive silencer factor
Biochem. Biophys. Res. Commun. 346 (2), 426-435 (2006)
Kim SM, Yang JW, Park MJ, Lee JK, Kim SU, Lee YS and Lee MA.


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Characterization of the REST/NRSF-interacting LIM domain protein (RILP): localization and interaction with REST/NRSF
J. Neurochem. 96 (4), 1130-1138 (2006)
Shimojo M and Hersh LB.


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REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor
Mol. Cell. Biol. 23 (24), 9025-9031 (2003)
Shimojo M and Hersh LB.


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Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle
Int. J. Mol. Med. 11 (2), 249-256 (2003)
Katoh M and Katoh M.


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PRICKLE1-Related Progressive Myoclonus Epilepsy with Ataxia
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Fox,M.H. and Bassuk,A.G.


 
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